CN111053057A - Zooplankton separation device and separation method - Google Patents
Zooplankton separation device and separation method Download PDFInfo
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- CN111053057A CN111053057A CN201911374848.XA CN201911374848A CN111053057A CN 111053057 A CN111053057 A CN 111053057A CN 201911374848 A CN201911374848 A CN 201911374848A CN 111053057 A CN111053057 A CN 111053057A
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- zooplankton
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- 238000000926 separation method Methods 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 32
- 239000000463 material Substances 0.000 claims description 10
- 239000012535 impurity Substances 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 12
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 239000004698 Polyethylene Substances 0.000 description 3
- -1 polyethylene Polymers 0.000 description 3
- 229920000573 polyethylene Polymers 0.000 description 3
- 229910000746 Structural steel Inorganic materials 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 230000029264 phototaxis Effects 0.000 description 2
- 229910052573 porcelain Inorganic materials 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 244000038293 primary consumers Species 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a zooplankton separation device and a separation method, wherein the device comprises a sample separation unit, a light source unit and a fixing frame, the sample separation unit comprises a sample separation cup, a zooplankton concentration cup and a hose which are arranged from top to bottom, the light source unit comprises a power supply box and a light source, the light source and the zooplankton concentration cup are arranged in the power supply box, the sample separation cup is detachably connected with the power supply box, the hose penetrates through the power supply box, and the power supply box is fixed on the fixing frame. Compared with the prior art, the invention has the following advantages: (1) the zooplankton separation device is simple in structure, is unitized or modularized, is simple and convenient to operate, is convenient to carry, is easy to replace and repair, is low in manufacturing cost, and can be made in an emergency and temporary manner on an acquisition site; (2) the device and the method can separate purer zooplankton, and have higher efficiency, thereby saving a large amount of time and manpower.
Description
Technical Field
The invention belongs to the technical field of ecological environment, and relates to a zooplankton separating and collecting device, in particular to a zooplankton separating device and a separating method.
Background
Zooplankton is a group of primitive organisms, is a primary consumer in the ecosystem of oceans, fresh water rivers and lakes, and is also an important aquatic organism resource. The micro zooplankton is distributed in each part of the global water system, has multiple types, large quantity, fast breeding and high economic value, and plays an extremely important role in the circulation of materials and energy of an ecological system.
Zooplankton is used more often, for example: the water quality control agent is used as bait food or used for detecting and counting in a laboratory so as to reflect the current situation of the local water environment; wherein, toxicity test, isotope detection and the like in a laboratory all need to use high-concentration pure zooplankton. How to simply and effectively separate and concentrate zooplankton becomes one of the bases for carrying out experiments by taking zooplankton as research material. The collection of zooplankton in the field water body has the following difficulties: 1. zooplankton is difficult to gather and collect; 2. the separation of zooplankton from phytoplankton and impurities in water is difficult; 3. the separation of zooplankton takes time and labor.
The existing common zooplankton separation method is that a white porcelain plate or a transparent plastic bottle is used for irradiating one foot of the porcelain plate by a flashlight under the dark condition, the phototaxis of the zooplankton is utilized, and the zooplankton is collected from the bottom by a rubber head dropper, so that the method is more complicated, the consumed time is long, the zooplankton is greatly influenced by the environment (under the dark condition), and the collected zooplankton is more in impurity.
Disclosure of Invention
The technical problem to be solved is as follows: in order to overcome the defects of the prior art and solve the problems of difficulty in collecting and separating zooplankton in a field water body, labor consumption and time consumption, the invention designs a simple and easy-to-operate purification and collection scheme which is suitable for the zooplankton in a field concentrated water body, and particularly relates to a zooplankton separation device and a separation method.
The technical scheme is as follows: zooplankton separator, the device includes sample separation unit, light source unit and mount, and sample separation unit includes sample separation cup, the concentrated cup of zooplankton and the hose that top-down set up, and light source unit includes power pack and light source, and in light source and the concentrated cup of zooplankton located the power pack, the connection can be dismantled to sample separation cup and power pack, and the hose passes the power pack, and the power pack is fixed in on the mount.
Preferably, the cup body of the sample separation cup is made of opaque light-proof materials.
Preferably, the power supply box and the sample separating cup are made of the same material.
Preferably, the light source is an LED light source, and the wattage and the number of the light source are adjusted according to the requirement of sample water separation.
Preferably, the zooplankton concentration cup is funnel-shaped, and the open face is fixedly connected with the sample separation cup.
Preferably, the power box comprises a power box shell, and the power box shell is divided into a left part and a right part which are connected by a buckle; the upper part of the power box is provided with an upper opening which is flush with the open face of the zooplankton concentration cup, and the lower part of the power box is provided with a lower opening for the hose to pass through. The hose is preferably a rubber tube with a diameter of 5mm, and the length of the hose can be adjusted.
Preferably, the bottom surfaces of the sample separating cup and the power supply box are the same. Furthermore, the sample separating cup and the power supply box are both cylinders and are made of opaque polyethylene.
Preferably, the power supply box is detachably connected with the fixing frame, and the fixing frame and the power supply box form an angle of 120 degrees. The mount preferably adopts three angle iron stands, and the stabilizer blade of three angle iron stands welds into circularly, increases the stability of device and plays the supporting role.
Preferably, a water stop clip is arranged below the hose. The water stop clamp preferably uses a spring water stop clamp commonly used in laboratories.
The separation method of the zooplankton separation device, which comprises the following steps:
s1, collecting sample water containing zooplankton, and removing impurities;
s2, matching the specification of the sample separating cup according to the total amount of the sample water in the S1 and the processing requirement, then selecting a power supply box with the same bottom specification as the sample separating cup, combining the power supply box and the sample separating cup, and fixing the power supply box and the sample separating cup on a fixing frame;
s3, pouring the sample water in the S1 into a sample separating cup, turning on a light source in a power box, turning on a water stop clip every 2-3min, controlling the flow at 5-10mL/min, and controlling the total separation time to be less than 15 min.
Has the advantages that: (1) the zooplankton separation device is simple in structure, is unitized or modularized, is simple and convenient to operate, is convenient to carry, is easy to replace and repair, is low in manufacturing cost, and can be made in an emergency and temporary manner on an acquisition site; (2) the device and the method can separate purer zooplankton, and have higher efficiency, thereby saving a large amount of time and manpower.
Drawings
Fig. 1 is a schematic structural view of the zooplankton separation device according to the present invention;
FIG. 2 is a top view of the battery case;
wherein, 1 is a sample separation cup, 2 is a battery box, 3 is a zooplankton concentration cup, 4 is a light source, 5 is a hose, 6 is a water stop clamp, 7 is a fixing frame, 8 is a power box shell, 9 is a buckle, 10 is an upper hole of the power box, and 11 is a lower hole of the power box.
Detailed Description
The following examples further illustrate the present invention but are not to be construed as limiting the invention. Modifications and substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit and substance of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
Example 1
Zooplankton separator, the device includes sample separation unit, light source unit and mount 7, and sample separation unit includes sample separation cup 1, zooplankton concentration cup 3 and the hose 5 that top-down set up, and the light source unit includes power pack 2 and light source 4, and in power pack 2 was located to light source 4 and zooplankton concentration cup 3, sample separation cup 1 and power pack 2 can be dismantled and connect, and hose 5 passed power pack 2, and power pack 2 is fixed in on the mount 7.
The cup body of the sample separation cup 1 is made of opaque light-proof materials.
The power box 2 and the sample separating cup 1 are made of the same material.
The zooplankton concentration cup 3 is funnel-shaped, and the open surface is fixedly connected with the sample separation cup 1.
The power box 2 comprises a power box shell 8, and the power box shell 8 is divided into a left part and a right part which are connected by a buckle 9; the upper part of the power box 2 is provided with a power box upper opening 10 which is flush with the open surface of the zooplankton concentration cup 3, and the lower part of the power box 2 is provided with a power box lower opening 11 for the hose 5 to pass through.
The bottom surfaces of the sample separating cup 1 and the power supply box 2 are the same.
The power supply box 2 is detachably connected with the fixed frame 7.
A water stop clamp 6 is arranged below the hose 5.
Example 2
The zooplankton separation device of embodiment 1 is adopted for separation, and comprises the following steps:
s1, collecting sample water containing zooplankton, and removing impurities;
s2, matching the specification of the sample separating cup 1 according to the total amount of the sample water in the S1 and the processing requirement, then selecting the power supply box 2 with the specification of the same bottom surface as that of the sample separating cup 1, combining the power supply box and the power supply box, and fixing the power supply box and the power supply box on a fixing frame 7;
s3, pouring the sample water in the S1 into the sample separation cup 1, turning on the light source 4 in the power box 2, turning on the water stop clamp 6 every 2-3min, controlling the flow at 5-10mL/min, and controlling the total separation time to be less than 15 min.
Example 3
The device described in example 1 was made portable, as follows:
the sample separating cup 1 is an opaque black cylinder polyethylene bottle with the diameter of 80mm and the height of 90mm, and the power supply box 2 is a cylinder with the diameter of 80mm and the height of 30mm and is made of the same material as the sample separating cup 1; the zooplankton concentrating cup is funnel-shaped, each side is 30mm, the diameter of a rubber tube connected with the lower end of the zooplankton concentrating cup is 5mm, and a transparent polyethylene material with good light transmittance is adopted; the light source 4 adopts two 0.05W LED lamps; the fixing frame 7 adopts a triangular iron frame, the length of the fixing frame is 60mm, an angle of 120 degrees is formed between the fixing frame and the power supply box, and three support legs of the triangular iron frame are welded into a circle. The power box 2 is divided into a left part and a right part on average and connected through a buckle 9, the diameter of an upper opening 10 of the power box is 30mm, and the diameter of a lower opening 11 of the power box is 5 mm.
Adopt zooplankton in this portable's zooplankton separator separation water, concrete step is as follows:
zooplankton in water is concentrated and separated by a certain lake area of the white lake in Hebei province and is used for isotope research. A No. 25 plankton net (200 meshes) is used for pulling for 0.5 to 1 hour at the sampling point in a ring shape at the speed of 1 m/s. During the period, the collected samples are stored in sample bottles (sample bottle openings) every 1-3 min. Pouring the collected sample containing zooplankton into a beaker, adding water of a sampling point filtered by a 0.45-micrometer filter membrane for dilution, and picking out larger impurities.
Pouring 500-800mL of diluted water sample into the sample separation cup 1, turning on the light source 4, moving the zooplankton to the zooplankton concentration cup 3 by utilizing the phototaxis of the zooplankton, and opening the water stop clamp 6 every 2-3min through visual observation to separate the zooplankton, wherein the flow is controlled to be 5-10mL each time. The whole separation operation is controlled within 15min, and phytoplankton and impurities in water are prevented from precipitating in a concentration cup. And repeating the steps to separate out pure zooplankton.
Claims (9)
1. Zooplankton separator, a serial communication port, the device includes sample separating element, light source unit and mount (7), sample separating element includes sample separating cup (1) that top-down set up, zooplankton concentration cup (3) and hose (5), the light source unit includes power pack (2) and light source (4), power pack (2) are located in light source (4) and zooplankton concentration cup (3), sample separating cup (1) and power pack (2) can be dismantled and be connected, hose (5) pass power pack (2), power pack (2) are fixed in on mount (7).
2. The zooplankton separation device of claim 1, characterized in that the cup body of the sample separation cup (1) is made of opaque and light-proof material.
3. The zooplankton separation device of claim 2, characterized in that the power box (2) and the sample separation cup (1) are made of the same material.
4. Zooplankton separation device according to claim 1, characterized in that the zooplankton concentrating cup (3) is funnel-shaped and the open face is fixedly connected with the sample separation cup (1).
5. The zooplankton separation device of claim 1, characterized in that the power box (2) comprises a power box casing (8), the power box casing (8) is divided into a left part and a right part and is connected by a buckle (9); an upper opening (10) of the power supply box is arranged above the power supply box (2) and is flush with the open surface of the zooplankton concentration cup (3), and a lower opening (11) of the power supply box is arranged below the power supply box (2) and is used for the hose (5) to pass through.
6. Zooplankton separation apparatus according to claim 1, characterized in that the bottom surfaces of the sample separation cup (1) and the power supply box (2) are identical.
7. Zooplankton separation device of claim 1, characterized in that the power pack (2) is detachably connected to the holder (7).
8. Zooplankton separation device according to claim 1, characterized in that a water stop clip (6) is provided below the hose (5).
9. A separation method for zooplankton separation apparatus according to any one of claims 1 to 8, characterized in that the method comprises the steps of:
s1, collecting sample water containing zooplankton, and removing impurities;
s2, matching the specification of the sample separating cup (1) according to the total amount of the sample water in the S1 and the processing requirement, then selecting a power supply box (2) with the specification of the same bottom surface as that of the sample separating cup (1), combining the power supply box and the power supply box, and fixing the power supply box and the power supply box on a fixing frame (7);
s3, pouring the sample water in the S1 into the sample separation cup (1), turning on a light source (4) in a power box (2), turning on a water stop clamp (6) every 2-3min, controlling the flow at 5-10mL/min, and controlling the total separation time to be less than 15 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911374848.XA CN111053057A (en) | 2019-12-27 | 2019-12-27 | Zooplankton separation device and separation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911374848.XA CN111053057A (en) | 2019-12-27 | 2019-12-27 | Zooplankton separation device and separation method |
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| CN111053057A true CN111053057A (en) | 2020-04-24 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112945691A (en) * | 2021-02-04 | 2021-06-11 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Zooplankton sample washing unit |
| CN117652487A (en) * | 2023-12-05 | 2024-03-08 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Device suitable for zooplankton keeps, washs and retrieves |
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| CN107570316A (en) * | 2017-08-03 | 2018-01-12 | 中国水产科学研究院南海水产研究所 | Seawater buoyancy fish-egg sieves excellent separation method and the separating barrel and the application method of separating barrel used |
| CN211581219U (en) * | 2019-12-27 | 2020-09-29 | 中国水产科学研究院淡水渔业研究中心 | Zooplankton separator |
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2019
- 2019-12-27 CN CN201911374848.XA patent/CN111053057A/en active Pending
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| CN102422828A (en) * | 2011-08-22 | 2012-04-25 | 中国科学院南海海洋研究所 | Sorting device for zooplankton living body samples |
| US20160312173A1 (en) * | 2014-02-06 | 2016-10-27 | Nancy Leland | Method and device for plankton separation |
| CN205161579U (en) * | 2015-12-03 | 2016-04-20 | 厦门金益海生物科技有限公司 | Fairy shrimp hatching apparatus convenient to worm's ovum separation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112945691A (en) * | 2021-02-04 | 2021-06-11 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Zooplankton sample washing unit |
| CN112945691B (en) * | 2021-02-04 | 2022-07-08 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Zooplankton sample washing unit |
| CN117652487A (en) * | 2023-12-05 | 2024-03-08 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Device suitable for zooplankton keeps, washs and retrieves |
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