CN107570316A - Seawater buoyancy fish-egg sieves excellent separation method and the separating barrel and the application method of separating barrel used - Google Patents

Seawater buoyancy fish-egg sieves excellent separation method and the separating barrel and the application method of separating barrel used Download PDF

Info

Publication number
CN107570316A
CN107570316A CN201710655869.3A CN201710655869A CN107570316A CN 107570316 A CN107570316 A CN 107570316A CN 201710655869 A CN201710655869 A CN 201710655869A CN 107570316 A CN107570316 A CN 107570316A
Authority
CN
China
Prior art keywords
egg
fish
separating
separating barrel
barrel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710655869.3A
Other languages
Chinese (zh)
Inventor
郭华阳
张殿昌
江世贵
张楠
朱克诚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
Original Assignee
South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences filed Critical South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
Priority to CN201710655869.3A priority Critical patent/CN107570316A/en
Publication of CN107570316A publication Critical patent/CN107570316A/en
Pending legal-status Critical Current

Links

Abstract

Seawater buoyancy fish-egg sieves excellent separation method and the separating barrel and the application method of separating barrel used, methods described comprise the following steps:Step 1:Inflation separation:Seawater is injected into separating barrel and is put into fish-egg to be separated, then inflates, makes fish-egg with pneumatic floating in water;Step 2:High salinity seawater is allocated using sea salt to be added in separating barrel, heightens seawater salinity;Step 3:Standing separation:Cover bung, the delivery port at the separating barrel bucket bottom is formed about unique transparent area, after standing, the impurity for being gathered in barrel bottom, dead ovum and zooplankter is isolated by the delivery port of the separating barrel, reclaims the fish-egg of floating, complete the sieve optimal sorting of fish-egg from.The inventive method is after initial gross separation is inflated, separation is inflated again in high salinity seawater by improving seawater salinity, can be promoted further separating for high-quality fish-egg and impurity, zooplankter and dead ovum, be accelerated high-quality fish-egg ascent rate, separating effect is lifted, accelerates separating rate.

Description

Seawater buoyancy fish-egg sieves the use of excellent separation method and the separating barrel and separating barrel used Method
Technical field
The present invention relates to fish-egg to sieve excellent separation field, and more particularly to a kind of seawater buoyancy fish-egg sieves excellent separation method and used Separating barrel and separating barrel application method.
Background technology
Fish are also different because of the characteristic of its different fish-egg of its species.Heavy property can be divided into according to its ovum ecological characteristic and proportion The types such as ovum, buoyant egg and drifting egg.Wherein the ovum of fish belongs to buoyant egg more.
At present, during induction in cage parent population, 80 mesh or the screen cloth of other size meshes are hung in more uses in net cage to be prevented Fish-egg is lost in.Buoyancy fish-egg is to float in the seawater under normal circumstances, fish-egg can be collected using dredger method.Collect Although fish-egg is largely the live fish ovum to float, be also mingled with dead ovum, at the same also suspended particulate, Copepods etc. swim it is dynamic Thing.Therefore, the sieve of fish-egg is excellent very necessary.
Traditional excellent processing of fish-egg sieve is mainly separated using fairy shrimp hatching barrel.Found through experiment, fairy shrimp hatching Bucket has an impact to the incubation rate of fish fish-egg.Because fish fish-egg egg membrane is relatively thin, and traditional fairy shrimp hatching barrel is with glass Steel fibre is material, because manufacture craft limitation causes a barrel inner wall coarse injustice, because air-flow drives in fish-egg separation, hatching process Easily cause fish-egg to scratch, influence hatching and the seed rearing of fish-egg.
In terms of excellent separation method is sieved, conventional method is roughly divided into two steps:1) inflation separation:Seawater is injected in hatching barrel And fish-egg to be separated is put into, and after inflation is stood, make to bubble through the water column on fish-egg, impurity and zooplankter (utilizing its phototaxis) aggregation At bucket bottom;2) standing separation:After standing, impurity (including dead ovum) and zooplankter are isolated, reclaims the fish-egg of floating, completes fish The sieve optimal sorting of ovum from.
The content of the invention
It is an object of the invention to provide a kind of seawater buoyancy fish-egg of good separating effect to sieve excellent separation method, and this method can root The impurity adulterated in fish-egg and zooplankter are more effectively isolated according to the characteristic of buoyancy fish-egg.
The present invention also provides separating barrel used in a kind of above method.
In addition, the present invention also provides a kind of specifically used method of above-mentioned separating barrel.
The first goal of the invention of the present invention is achieved through the following technical solutions:A kind of sieve excellent separation method of buoyancy fish-egg, Comprise the following steps:
Step 1:Inflation separation:Seawater is injected into separating barrel and is put into fish-egg to be separated, then inflates, makes fish-egg with gas Stream suspends in water;
Step 2:High salinity seawater is allocated using sea salt to be added in separating barrel, heightens seawater salinity, and ensure separating barrel In seawater salinity in the tolerance range of fish-egg development hatching, heighten seawater salinity, to increase buoyant of sea water, accelerate unblemished fish Ovum separating rate;
Step 3:Standing separation:Bung is covered, the delivery port at the separating barrel bucket bottom is formed about unique transparent area, quiet Postpone, the impurity for being gathered in barrel bottom, dead ovum and zooplankter (driving photosensitiveness using it) isolated by the delivery port of the separating barrel, Reclaim the fish-egg to float, complete the sieve optimal sorting of fish-egg from.
Step 2 is carried out in the deflated condition.
In step 1, husky filter processing need to be passed through by adding the seawater of separating barrel, and is ensured that the seawater of addition meets and developed by fish-egg Water quality requirement needed for hatching.
In step 2, high salinity seawater is allocated using sea salt, is then added to after the filtering of the 300 mesh string bags in separating barrel.
Step 1 inflationtime is 10min-20min.
In step 2, make seawater salinity rise 3 ‰ -4 ‰ in separating barrel.
Whole sieve optimal sorting is controlled within 1h from process time, in order to which later stage fish-egg transports.
Second goal of the invention of the present invention is achieved through the following technical solutions:Used separation in a kind of above method Bucket, including the separating barrel main body equipped with bung, the separating barrel main body is made up of acrylic material forms smooth inwall, described Separating barrel main body is supported and liftoff setting by bucket pin, and the separating barrel bottom part body is in funnel-form, and the delivery port of bottom nearby uses Transparent material, after bung is covered, transparent area only is formed about in the delivery port, the separating barrel also includes outlet pipe, filled Tracheae, nanometer tracheae and for being inserted in the delivery port so as to block the separating pipe of the delivery port, the nanometer tracheae installation In the lower end of the separating pipe, the gas tube is connected with the nanometer tracheae, and the outlet pipe is arranged on the separating barrel master Below body, its upper end tube nozzle is communicated with the delivery port, and the outlet pipe is made of clear material, and equipped with switch.
The separating barrel main body is formed by acrylic mold, can form smooth inwall, is reduced in fish-egg separation process Damage.
Other parts and the bung of the ladle body of the separating barrel main body in addition to the transparent area are that black is light tight.
The bucket bottom of the separating barrel main body and vertical direction angular range be 50-70 degree, and angle is smaller, zooplankter, greatly Granule foreign and dead ovum congregational rate are better.
The 3rd goal of the invention of the present invention is achieved through the following technical solutions:A kind of application method of the separating barrel, Comprise the following steps that:
1) switch of bottom of the barrel outlet pipe is closed, inserts separating pipe, seawater is added, fish-egg to be separated is put in seawater, And inflated by the nanometer tracheae of separating pipe lower end into water, make embryonated egg with pneumatic floating in water;
2) allocate high salinity seawater using sea salt to add in separating barrel, improve seawater salinity in separating barrel;
3) stop inflation, extract separating pipe, cover bung, stand a period of time, settleability impurity and dead fish ovum are because of gravity Effect is deposited in a barrel bottom, and zooplankter observes separation because phototaxis is also gathered in a barrel bottom, according to the transparent region of separation bottom of the barrel Situation, the switch of the outlet pipe of separation bottom of the barrel is opened, discharge is gathered in zooplankter, impurity and the dead ovum at barrel bottom, through more After secondary standing separation, fish-egg is collected using the bolting silk string bag.
Compared with prior art, the present invention has following significant effect:
(1) the inventive method is carried out again after initial gross separation is inflated by improving seawater salinity in high salinity seawater Inflation separation, can promote further separating for high-quality fish-egg and impurity, zooplankter and dead ovum, accelerate high-quality fish-egg float it is fast Degree, separating effect is lifted, accelerate separating rate;
(2) separating barrel of the present invention uses acrylic material, and the separating barrel main body inner wall of formation is smooth, can reduce sieve optimal sorting from During damage to fish-egg, be advantageous to improve incubation rate;Separating barrel main body of the present invention is made up of acrylic material, transparent area Transparency is preferable, is more beneficial for the aggregation situation that thing is mixed in observation.
Brief description of the drawings
Fig. 1 is the structural representation of the separating barrel of the specific embodiment of the invention;
Fig. 2 is separating barrel polycrystalline substance schematic diagram;
In figure:1- handles;2- bungs;3- separating barrel main bodys;5- separating pipes;6- circular nano tracheaes;7- delivery ports;8- points From bucket bottom;9- outlet pipes;10- is switched;11- gas tubes;12- bucket pin;13- transparent circles;14- black circles.
Embodiment:
For ease of it will be appreciated by those skilled in the art that below in conjunction with the accompanying drawings and specific embodiment to the present invention make further it is detailed Describe in detail bright.
As shown in Fig. 1~2, the progress seawater buoyancy fish-egg of the present embodiment sieve optimal sorting from separating barrel include being furnished with bung 2 Separating barrel main body 3, bung 2 is provided with handle 1.
Bung 2 is black, light tight, and medial surface is provided with panel in the annular suitable with the internal diameter of separating barrel main body 3, to stop Light enters.
Separating barrel main body 3 is made up of acrylic, and in cylinder, outer wall is in black, such as coats shading coating, bucket bottom 8 is in leakage Bucket-shaped, the periphery of delivery port 7 sets the circular non-opaque circle 13 of certain diameter, forms transparent area, and other regions of bottom surface are also black, shape Into black circle 14.With separating barrel main body 3 with high separating pipe 5, separating pipe 5 is PVC for the inserting of delivery port 7 in separating barrel main body 3 Pipe, its lower end suit ring-type nanometer tracheae 6.Circular nano pipe 6 is connected with gas tube 11.One section of the lower section of separating barrel 8 setting is transparent Acrylic outlet pipe 9, the upper end tube nozzle of outlet pipe 9 connect with delivery port 7, and outlet pipe 9 sets switch 10.Bucket pin 12 supports separating barrel 3 liftoff setting of main body.
In the present embodiment, the diameter of separating barrel main body 3 is 80cm, and bucket edge to bottom of the barrel water outlet height is 80cm, maximum Volume is 400L, and bucket bottom and bucket pin are in 70 ° of angles, and 7 a diameter of 20mm of delivery port, the length of outlet pipe 9 is 15cm, and bucket pin is highly 50cm.The height of separating pipe 5 is 80cm.
The course of work of the present invention is as follows:After fish-egg is collected from sea area, fish-egg process chamber is delivered in time.In fish-egg processing Room, after separating barrel sterilization is cleaned, the switch of separation bottom of the barrel outlet pipe is closed, inserts separating pipe, and the ring that bottom of the tube will be separated The connection inflation of shape nanometer tracheae.Water inlet switch is opened, adds the nature seawater by husky filter.By the optimal sorting to be sieved of collection from fish Ovum is put into separating barrel, opens charging valve, makes fish-egg with pneumatic floating in bucket, and high-quality fish-egg is by normal development.Inflation After 10min-20min, high salinity seawater is allocated using sea salt, is added after the filtering of the 300 mesh string bags in separating barrel, rise 3 ‰- 4 ‰ seawater salinities are so as to increasing the buoyancy of high-quality fish-egg.Charging valve is closed after inflation is well mixed, after standing some minutes, Separating pipe is gently extracted, covers bung.After standing a period of time, separation situation is observed by separating barrel bottom transparent region, waits to swim After animal, large granular impurity and dead ovum are gathered in separating barrel bottom, the switch of separation bottom of the barrel outlet pipe is opened, discharge is swum dynamic Thing, large granular impurity and dead ovum.By multiple standing separation, after dead ovum is discharged through bottom, high-quality fish-egg can utilize 100 mesh sieves Tulle pocket is collected, whole to optimize disengaging time control within 1h, in order to which later stage fish-egg transports.
The present invention operation principle be:Fish-egg is placed in separating barrel after collecting, and is inflated using circular nano tracheae and is driven fish Ovum, after inflating certain time, high-quality fish-egg normal development.Add certain salinity and heighten seawater salinity, the rise of seawater salinity High-quality fish-egg buoyancy will be increased, accelerate its ascent rate.After stopping the supple of gas or steam, separating pipe is extracted, separation bung is covered, with reference to zooplankter Phototaxis, the settleability of large granular impurity and dead ovum, promote zooplankter to be gathered in separating barrel bottom transparent circle position, while greatly Granule foreign, dead ovum are gathered in delivery port because of Action of Gravity Field.By opening the switch of separation bottom of the barrel by zooplankter, bulky grain Outside impurity and dead ovum discharge bucket.Operated by separating for several times, separate and obtain high-quality fish-egg.
The implementation of the present invention is not limited to this, according to the above of the present invention, according to ordinary skill knowledge And customary means, the big I of separating barrel of the invention are adjusted as needed;Angle between separating barrel bottom and bucket pin is 50 ° -70 °, angle angle is smaller, and dead ovum, zooplankter and other impurities congregational rate are better, are more beneficial for separating;Separation The big I of bucket bottom delivery port, separating pipe and outlet pipe adjusts as needed, and delivery port is smaller, and separating effect is better.Separation Stave wood matter and shape also have other embodiments;Therefore, under the premise of the above-mentioned basic fundamental thought of the present invention is not departed from, this Invention can make the modification, replacement or change of other diversified forms, all should fall within the scope of this invention.

Claims (9)

1. the excellent separation method of sieve of a kind of buoyancy fish-egg, it is characterised in that comprise the following steps:
Step 1:Inflation separation:Seawater is injected into separating barrel and is put into fish-egg to be separated, then inflates, fish-egg is hanged with air-flow Float in water;
Step 2:High salinity seawater is allocated using sea salt to be added in separating barrel, heightens seawater salinity;
Step 3:Standing separation:Bung is covered, the delivery port at the separating barrel bucket bottom is formed about unique transparent area, stands Afterwards, the impurity for being gathered in barrel bottom, dead ovum and zooplankter are isolated by the delivery port of the separating barrel, reclaims the fish-egg of floating, Complete fish-egg sieve optimal sorting from.
2. the excellent separation method of sieve of buoyancy fish-egg according to claim 1, it is characterised in that step 2 is in the deflated condition Carry out;
In step 1, husky filter processing need to be passed through by adding the seawater of separating barrel;
In step 2, high salinity seawater is allocated using sea salt, is then added to after the filtering of the 300 mesh string bags in separating barrel.
3. the excellent separation method of sieve of buoyancy fish-egg according to claim 1, it is characterised in that step 1 inflationtime is 10min-20min。
4. the excellent separation method of sieve of buoyancy fish-egg according to claim 1, it is characterised in that in step 2, make in separating barrel Seawater salinity rise 3 ‰ -4 ‰.
5. the excellent separation method of sieve of buoyancy fish-egg according to claim 1, it is characterised in that when entirely sieving excellent separation process Between control within 1h.
6. the used separating barrel in a kind of any one of Claims 1 to 5 claim methods described, it is characterised in that Including the separating barrel main body equipped with bung, the separating barrel main body is made up of acrylic material forms smooth inwall, described point Supported and liftoff setting by bucket pin from bucket main body, the separating barrel bottom part body is in funnel-form, and the delivery port of bottom is nearby using saturating Bright material, after bung is covered, transparent area only is formed about in the delivery port, the separating barrel also includes outlet pipe, inflation Pipe, nanometer tracheae and for being inserted in the delivery port so as to block the separating pipe of the delivery port, the nanometer tracheae is arranged on The lower end of the separating pipe, the gas tube are connected with the nanometer tracheae, and the outlet pipe is arranged on the separating barrel main body Lower section, its upper end tube nozzle are communicated with the delivery port, and the outlet pipe is made of clear material, and equipped with switch.
7. separating barrel according to claim 6, it is characterised in that the ladle body of the separating barrel main body is in addition to the transparent area Other parts and the bung be that black is light tight.
8. separating barrel according to claim 6, it is characterised in that the bucket bottom of the separating barrel main body and vertical direction angle Scope is 50-70 degree.
9. the application method of the separating barrel in a kind of any one of claim 6~8 claim, it is characterised in that specific Step is as follows:
1) switch of bottom of the barrel outlet pipe is closed, inserts separating pipe, seawater is added, fish-egg to be separated is put in seawater, and is led to The nanometer tracheae for crossing separating pipe lower end is inflated into water, makes embryonated egg with pneumatic floating in water;
2) allocate high salinity seawater using sea salt to add in separating barrel, improve seawater salinity in separating barrel;
3) stop inflation, extract separating pipe, cover bung, stand a period of time, settleability impurity and dead fish ovum are because of Action of Gravity Field A barrel bottom is deposited in, zooplankter observes separation situation because phototaxis is also gathered in a barrel bottom, according to the transparent region of separation bottom of the barrel, The switch of the outlet pipe of separation bottom of the barrel is opened, discharge is gathered in zooplankter, impurity and the dead ovum at barrel bottom, through repeatedly standing After separation, fish-egg is collected using the bolting silk string bag.
CN201710655869.3A 2017-08-03 2017-08-03 Seawater buoyancy fish-egg sieves excellent separation method and the separating barrel and the application method of separating barrel used Pending CN107570316A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710655869.3A CN107570316A (en) 2017-08-03 2017-08-03 Seawater buoyancy fish-egg sieves excellent separation method and the separating barrel and the application method of separating barrel used

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710655869.3A CN107570316A (en) 2017-08-03 2017-08-03 Seawater buoyancy fish-egg sieves excellent separation method and the separating barrel and the application method of separating barrel used

Publications (1)

Publication Number Publication Date
CN107570316A true CN107570316A (en) 2018-01-12

Family

ID=61034499

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710655869.3A Pending CN107570316A (en) 2017-08-03 2017-08-03 Seawater buoyancy fish-egg sieves excellent separation method and the separating barrel and the application method of separating barrel used

Country Status (1)

Country Link
CN (1) CN107570316A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108896352A (en) * 2018-06-13 2018-11-27 浙江省海洋水产研究所 A kind of fixed point monitoring method of ocean fish-egg
CN109757413A (en) * 2019-03-26 2019-05-17 中国水产科学研究院黑龙江水产研究所 A kind of field acquisition floatability fish-egg biopsy sample ease ovum suit separator and separation method
CN109892260A (en) * 2019-04-19 2019-06-18 威海泰丰海水育苗有限公司 A kind of separation method of round spot halibut superiority and inferiority matter fertilized eggs

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201541590U (en) * 2009-10-28 2010-08-11 广东海洋大学 Novel fish hatcher
CN202979902U (en) * 2013-01-17 2013-06-12 中国水产科学研究院黑龙江水产研究所 Burbot fertilized egg incubator
DE202013102728U1 (en) * 2013-06-24 2013-07-04 Ruhrverband Anfütterungsbecken
CN205161579U (en) * 2015-12-03 2016-04-20 厦门金益海生物科技有限公司 Fairy shrimp hatching apparatus convenient to worm's ovum separation
CN205547035U (en) * 2016-04-14 2016-09-07 饶平县施蓝水处理科技有限公司 Fairy shrimp separator
CN206005570U (en) * 2016-08-31 2017-03-15 苏州市申航生态科技发展股份有限公司 A kind of de- glutinous and hatching apparatus of stickiness fish-egg

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201541590U (en) * 2009-10-28 2010-08-11 广东海洋大学 Novel fish hatcher
CN202979902U (en) * 2013-01-17 2013-06-12 中国水产科学研究院黑龙江水产研究所 Burbot fertilized egg incubator
DE202013102728U1 (en) * 2013-06-24 2013-07-04 Ruhrverband Anfütterungsbecken
CN205161579U (en) * 2015-12-03 2016-04-20 厦门金益海生物科技有限公司 Fairy shrimp hatching apparatus convenient to worm's ovum separation
CN205547035U (en) * 2016-04-14 2016-09-07 饶平县施蓝水处理科技有限公司 Fairy shrimp separator
CN206005570U (en) * 2016-08-31 2017-03-15 苏州市申航生态科技发展股份有限公司 A kind of de- glutinous and hatching apparatus of stickiness fish-egg

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108896352A (en) * 2018-06-13 2018-11-27 浙江省海洋水产研究所 A kind of fixed point monitoring method of ocean fish-egg
CN108896352B (en) * 2018-06-13 2020-08-14 浙江省海洋水产研究所 Fixed-point monitoring method for marine fish eggs
CN109757413A (en) * 2019-03-26 2019-05-17 中国水产科学研究院黑龙江水产研究所 A kind of field acquisition floatability fish-egg biopsy sample ease ovum suit separator and separation method
CN109757413B (en) * 2019-03-26 2021-07-27 中国水产科学研究院黑龙江水产研究所 Sleeved separator for field collection of floating roe living body sample egg escape and separation method
CN109892260A (en) * 2019-04-19 2019-06-18 威海泰丰海水育苗有限公司 A kind of separation method of round spot halibut superiority and inferiority matter fertilized eggs

Similar Documents

Publication Publication Date Title
CN105393975B (en) A kind of simple recirculated water shrimp culture pond water treatment system
CN205518799U (en) Red ball algae belt cleaning device is given birth to to rain
CN107570316A (en) Seawater buoyancy fish-egg sieves excellent separation method and the separating barrel and the application method of separating barrel used
CN210617030U (en) Micro-plastic flotation device
CN206734570U (en) A kind of pick-up boat that cleaning water body recovery technology is aspirated by pressure difference
CN110226558A (en) Dirt and dead shrimp shrimp shell separates and collects device in industrial circulating water prawn culturing
CN109855930A (en) Micro- plastic selector and separation method in a kind of soil
CN206095699U (en) Fishing rod formula sampling device
CN208902511U (en) Phytoplankton sample concentration device
CN102257976A (en) Clam seedling catching device
CN211020558U (en) Inflatable shellfish larva water changing net cage device
CN204191380U (en) A kind of shrimps circulation staged cultivator
CN206686954U (en) The portable separator of indoor artemia hatching
CN212476343U (en) Aquaculture biological wadding screening device
CN105084568B (en) Oil-contained waste water treatment device and handling process
CN108112550A (en) The suction loach pumping unit of adjustable water absorption
CN105819587B (en) A kind of floated equipment that can separate soluble debirs in sea-farming
CN208001873U (en) A kind of device of quick separating silvery pomfret quality fertilized eggs
CN206295638U (en) A kind of inclined-plate clarifying basin and its mud discharging mechanism
CN207476916U (en) A kind of sesame cleaning filter
CN205662435U (en) Sewage treatment system is bred to shrimp
CN108064812A (en) Anticlogging jet stream clingfish pumping unit
CN205786018U (en) A kind of nematicide segregation apparatus
CN205547035U (en) Fairy shrimp separator
CN202983301U (en) Automatic micro-powder sorting equipment

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20180112