CN109757413A - A kind of field acquisition floatability fish-egg biopsy sample ease ovum suit separator and separation method - Google Patents
A kind of field acquisition floatability fish-egg biopsy sample ease ovum suit separator and separation method Download PDFInfo
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- CN109757413A CN109757413A CN201910232790.9A CN201910232790A CN109757413A CN 109757413 A CN109757413 A CN 109757413A CN 201910232790 A CN201910232790 A CN 201910232790A CN 109757413 A CN109757413 A CN 109757413A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
A kind of field acquisition floatability fish-egg biopsy sample ease ovum suit separator and separation method, it is related to a kind of fish-egg sample living body separator and separation method, the problem of the purpose of the present invention is to solve field acquisition fish-egg living body is lossless or the separation of micro- damage, the device of the invention can will collect the living body fish-egg for producing floatability ovum fish (containing prelarva in field, similarly hereinafter) sample, according to the buoyancy and grain size specification of fish-egg, it takes and passes through water body flow or rotation in sample container (separator) or the method separating fish-egg sample for removing fish-egg freely in floating and rotation from escape hole that floats at investigation scene, fish-egg sample cluster is set to isolate the sample process unit of different-grain diameter, the original mixed sample of acquisition is carried out to a kind of screening plant of separation classification, the present invention is applied to fishery field.
Description
Technical field
The present invention relates to a kind of fish-egg sample living body separator and separation methods.
Background technique
In fishery resources and the investigation of fish spawning ground, in addition to the fish sample of acquisition different size and age, also
Need to acquire fish-egg and prelarva sample, to speculate that position, early stage resource occurrence quantity, stock enhancement and the various fish in spawning ground produce
Ovum scale etc..Fish are mostly in vitro fertilization, i.e., when Spawning Ground Ecologic condition meets and breeds, male and female adult fish is being bred
It is mutually chased under physiological hormone effect while mature egg and sperm is excluded into external completion fertilization, fertilized eggs are hatched and opened in water
Beginning ontogenetic process.The morphosis property of different fish mature eggs and fertilized eggs is different.It is divided into drift according to buoyancy
Property ovum, buoyant egg and demersal egg and adhesive egg, while being distinguished there are also size specification, mature egg is in spherical, and diameter is generally in 0.5-
6.0mm etc..For Drifting egg and buoyant egg (referred to as floating ovum), since fish-egg and prelarva do not have Burden-Swimming Ability of KM still, i.e.,
It can only flow downstream and float and lower or diffusion from spawning ground breeding area in river or lake and reservoir, ascending current is floating fish-egg
The continuous water swelling of egg membrane in the process, ovum diameter increase, and embryo gradually hatches development, final rupture of membranes and out to newly hatched larvae after supervention
It educates, until having fin and fish glue, it is movable with forage etc. just to carry out actively swimming.Condition of living organism is spherical in shape in water for fish-egg, ovum diameter
Size is the important indicator of fish-egg sample collection and taxonomic identification.It is flowed against the current with fish-egg and prelarva investigation application specific standard acquisition netting gear
Fish-egg and newly hatched larvae are collected in direction, can acquire ovum diameter in water body and be greater than the various fish-eggs of 0.5mm or more and its prelarva.It is used when investigation
Acquire netting gear timing positioning acquisition sample.It focuses mostly in rivers and lakes Mesichthyes breeding period in spring and summer, the fish-egg of various fish
Often occur simultaneously with prelarva, the sample of acquisition is also all the mixing sample of various fish, while being also mingled in water body
Suspended matter or floating body.Sample and the sundries of entrainment are pulled out from acquisition net in investigation scene and are put into sample container, and
The visible sundries of naked eyes is chosen and is discarded, the preliminary treatments such as fish-egg and the morphologic observation of prelarva sample and sampling counting are then carried out, but
It is to be difficult to carry out the work such as living body separation, measurement, classification, counting and statistical disposition to various fish fish-eggs at the scene.While by
It is very thin after the egg membrane expansion of fish-egg, it is easily broken, copying fishing by hand, classification processing is easy to damage fish-egg in batches, and time-consuming takes
Power, it is easily dead from fish-egg after water.Since field investigation field condition is simple and crude, sample cannot first be carried out to living body separation fine processing
It is saved again afterwards, fishing extraction section mixing sample can only be copied by hand at present and fix (formalin or alcohol fixation liquid), taken back
Further taxonomic identification and statistics are done in laboratory, and fish-egg sample is due to dead, fish-egg color fade, fish-egg shape after fixing
Shape changes, and ovum diameter has changed, and loses the information data of vivo observation taxonomic identification, due to the classification difficulty of fish-egg and prelarva
Greatly, cause survey data data not enough science complete, the functional characteristic of biopsy sample is difficult to discover and use, in some instances it may even be possible to lose
It was found that the opportunity of rare preciousness species.
Summary of the invention
The problem of the purpose of the present invention is to solve field acquisition fish-egg living body is lossless or the separation of micro- damage, and one kind is provided
Field acquisition floatability fish-egg biopsy sample ease ovum suit separator and separation method.
A kind of field acquisition floatability fish-egg biopsy sample ease ovum of the invention is set with separator, it includes holding barrel, ease
Ovum bucket, water-supply-pipe, lid, water butt, appendix and air pump；The ease ovum bucket is made of the different ease ovum bucket of multiple internal diameters,
The ease ovum bucket of relatively small internal diameter is placed in relatively large ease ovum bucket；It ease ovum bucket bottom and covers and offers water delivery pipe through-hole respectively
One and water delivery pipe through-hole two, water-supply-pipe one end sequentially passes through the water delivery pipe through-hole on the water delivery pipe through-hole two covered and ease ovum bucket
One, it is placed in holding barrel bottom；Be provided with multiple water outlets on water-supply-pipe, the water inlet of water outlet by water-supply-pipe respectively with storage
The water outlet of bucket is connected to, and water outlet is located in ease ovum bucket；Water-supply-pipe in holding barrel is more piece tower structure, lower end
It is placed in holding barrel bottom；Internal diameter increases the water-supply-pipe of tower structure section by section from top to bottom；Every section water-supply-pipe and an adjacent upper section
Water-supply-pipe forms tower shoulder；Tower shoulder is provided with ease ovum bucket deck, and ease ovum bucket is placed on ease ovum bucket deck；
The tower shoulder of different inner diameters is equipped with diversion trench, and ease micropyle, and the opposite big ease ovum of internal diameter are provided in diversion trench
The aperture for the ease micropyle being arranged on bucket is greater than the aperture for the ease micropyle being arranged on the opposite small ease ovum bucket of internal diameter；Holding barrel and lid
Between be provided with spillway hole；It is placed on holding barrel, and by being connected on holding barrel；Lid is provided with multiple slots, and escape ovum bucket
Upper end is placed in slot；
Wherein, the aperture of spillway hole and relief hole is respectively less than rear fish-egg Sample diameter to be processed；
Exhaust outlet is set in ease ovum bucket, is covered and is offered gas transmission pipe through-hole, the air inlet of exhaust outlet passes through
It is connected to across the appendix of gas transmission pipe through-hole with the gas outlet of air pump.
A kind of application method of field acquisition floatability fish-egg biopsy sample ease ovum suit separator of the invention, it includes
Following steps:
Firstly, the ease ovum bucket of different inner diameters is sequentially placed according to the sequence of internal diameter from large to small；Then, by water-supply-pipe one
End sequentially passes through the water delivery pipe through-hole one on the water delivery pipe through-hole two covered and ease ovum bucket, is placed in holding barrel bottom, the other end
It is connected to the water outlet of water butt；The ease ovum bucket of different inner diameters is placed on ease ovum bucket deck again, and guarantees different inner diameters
Water outlet is distributed in ease ovum bucket；Ease ovum bucket is placed in holding barrel；Secondly, exhaust outlet is placed in the smallest ease of internal diameter
In ovum bucket, the air inlet of exhaust outlet is connected to by passing through the appendix of gas transmission pipe through-hole with the gas outlet of air pump；Again, will
Fish-egg sample is put into the smallest ease ovum bucket of internal diameter after to be processed；Finally, lid is placed on holding barrel, and by buckle and contain
The connection of sample bucket；The effluent switch for first opening water butt, by water outlet water flowing into ease ovum bucket, again by air pump and electric power storage when needing
Pond is connected, and is opened air pump to ease ovum bucket gas transmission, that is, is completed the field acquisition floatability fish-egg biopsy sample suit ease ovum group
The use of box-like separator.
The device of the invention can will collect the living body fish-egg (containing prelarva, similarly hereinafter) for producing floatability fish-egg fish in field
Sample, according to the buoyancy and grain size specification of fish-egg, take at investigation scene through water body flow in sample container (separator) or
Rotation or the method separating fish-egg sample for removing fish-egg freely in floating and rotation from escape hole that floats, make fish-egg sample cluster
The original mixed sample of acquisition is carried out a kind of screening plant of separation classification by the sample process unit for isolating different-grain diameter.
Apparatus of the present invention are suit ease ovum bucket Combined separator, and holding barrel and ease ovum bucket are not lure steel or plastic material,
Separator appearance is integrally in cylindrical body, and by being placed on holding barrel, lid is further solid by being connected in holding barrel outer wall
It is fixed.It is formed and is had the gap between holding barrel and lid, as spillway hole, after releasing in holding barrel due to full water, the pressure of formation,
Also for leaked water after holding barrel full water, is made, circulating water is formed, spillway hole can play the role of dispersing water flow direction,
Fish-egg can be driven to be uniformly distributed in ease ovum bucket bottom, separated convenient for fish-egg.The ovum bucket that escapes is U-shaped staving, and side wall is streamlined
Conducive to water body flow；It is made of the ease ovum bucket of multiple and different internal diameters, by the sequence of internal diameter from small to large, by the ease of small internal diameter
Ovum bucket is placed in the ease ovum bucket of large diameter；Each ease ovum bucket bottom offers water delivery pipe through-hole one, and water-supply-pipe passes through water-supply-pipe
Through-hole one is placed in the ease ovum bucket of different inner diameters, and ease ovum bucket deck is mounted on water-supply-pipe, is used to support different inner diameters
Escape ovum bucket, makes to form certain space between the ease ovum bucket of different inner diameters, can store different size of fish-egg.Tower shoulder is equipped with
Diversion trench, escape hole are laid in diversion trench.The identical escape hole in multiple apertures is distributed with (according to more in each ease ovum bucket bottom
Number fish ovum diameter Distribution Data, ease ovum bucket escape hole are followed successively by 4.5,3.5,2.5,1.5,0.5mm from large to small), for sieving
Same particle size fish-egg.It in order to further prevent fish-egg to collect around in escape hole, results in blockage, it may be necessary to use the present invention
Air pump is connected to by the air-blowing device of design by gas-guide tube with exhaust outlet, and exhaust outlet, which is placed in, prevents fish-egg sample
Ease ovum bucket in, after air pump is connect with battery, to ease ovum bucket discharge gas, generate bubble, formation stirred similar to field water
Dynamic, the effect of rolling can be such that fish-egg is separated.Wherein, water-supply-pipe of the invention and water delivery pipe through-hole two and water-supply-pipe are logical
The gap in hole one is less than the minimum diameter of fish-egg to be separated, preferably in 0.1mm or so.The aperture of spillway hole and relief hole is small
In the fish-egg sample minimum diameter after to be processed.The height of water butt of the invention is higher than the height of ease ovum bucket, to have certain
Difference in height, just can guarantee in this way, the water energy in water butt be enough flowed into ease ovum bucket in.Used water energy is enough from fish to be separated
The waters of ovum sample acquires.
The present invention include it is following the utility model has the advantages that
Separator and method for separating and processing of the present invention solve field acquisition fish-egg living body it is lossless or it is micro- damage separation
Problem carries out vivo observation research for investigation scene and provides sample, and combined type is set with separate design, time saving and energy saving, can seperated group
Dress, it is easy to carry, it is suitble to the operation of the banks unregulated power such as field riverbank or loke shore.Essence can be carried out to sample after the separation of fish-egg living body
The correlative studys such as quasi- classification and subsequent biological and stock enhancement protection.Vivo observation research is carried out for investigation scene, and sample is provided
This, combined type be set with separate design, it is time saving and energy saving, can separable assembled, it is easy to carry, be suitble to the banks such as field riverbank or loke shore without
Electric power operation.
Detailed description of the invention
Fig. 1 is cyclone separator arrangement schematic diagram of the present invention；
Fig. 2 is separator middle cover structure sectional view of the present invention.
Specific embodiment
Specific embodiment 1: illustrating present embodiment, a kind of field acquisition floatability fish-egg living body sample in conjunction with Fig. 1 to 2
This ease ovum is set with separator, holding barrel 1, ease ovum bucket 2, water-supply-pipe 3, lid 4, water butt 5, appendix 14 and air pump 15；
The ease ovum bucket 2 is made of the different ease ovum bucket of multiple internal diameters, and the ease ovum bucket of relatively small internal diameter is placed in relatively
In big ease ovum bucket；Two 6-2 of one 6-1 of water delivery pipe through-hole and water delivery pipe through-hole is offered respectively on ease 2 bottom of ovum bucket and lid 4,
3 one end of water-supply-pipe sequentially passes through one 6-1 of water delivery pipe through-hole on two 6-2 of water delivery pipe through-hole and ease ovum bucket 2 on lid 4, is placed in
1 bottom of holding barrel；Be provided with multiple water outlets 7 on water-supply-pipe 3, the water inlet of water outlet 7 by water-supply-pipe respectively with water butt 5
Water outlet connection, and water outlet 7 be located at ease ovum bucket 2 in；Water-supply-pipe 3 in holding barrel 1 is more piece tower structure, lower end
It is placed in 1 bottom of holding barrel；Internal diameter increases the water-supply-pipe 3 of tower structure section by section from top to bottom；Every section water-supply-pipe 3 and adjacent upper one
It saves water-supply-pipe 3 and forms tower shoulder；Tower shoulder is provided with ease ovum bucket deck 10, and ease ovum bucket 2 is placed on ease ovum bucket deck 10；
2 side wall of ease ovum bucket of different inner diameters is equipped with diversion trench 8, ease micropyle 9 is provided in diversion trench 8, and relatively interior
The aperture for the ease micropyle 9 being arranged on the big ease ovum bucket 2 of diameter is greater than the hole for the ease micropyle 9 being arranged on the opposite small ease ovum bucket 2 of internal diameter
Diameter；Spillway hole 16 is provided between holding barrel 1 and lid 4；Lid 4 is placed on holding barrel 1, and is connected in holding barrel 1 by buckle 12
On；Lid 4 is provided with multiple slots 18, and ease 2 upper end of ovum bucket is placed in slot 18；
Wherein, the aperture of spillway hole 11 and relief hole 16 is respectively less than rear fish-egg Sample diameter to be processed；
Exhaust outlet 17 is set in ease ovum bucket 2, offers gas transmission pipe through-hole 13 on lid 4, exhaust outlet 17 into
Port is connected to by passing through the appendix 14 of gas transmission pipe through-hole 13 with the gas outlet of air pump 15.
One 6-1 of water delivery pipe through-hole, two 6-2 of water delivery pipe through-hole and through-hole 13 of the invention and water-supply-pipe 3, appendix 14 it
Between be provided with adhesive tape, play the role of sealing.
Ease ovum bucket water outlet 7 of the invention is to be threadedly coupled with water-supply-pipe 3, easy to disassemble, after removing water outlet 7, just
It is separated from water-supply-pipe 3 in by the ease ovum bucket of different inner diameters, exhaust outlet 17 is also to be threadedly coupled with appendix 14, convenient for tearing open
It unloads, after exhaust outlet 17, convenient for separating the ease ovum bucket of different inner diameters from appendix 14.
The water-supply-pipe 3 of present embodiment is in 1 center of holding barrel, and the water-supply-pipe 3 in holding barrel 1 is more piece tower tube
Shape body, every section is equipped with several water outlets, and is equipped with tower shoulder, and tower shoulder increases step by step from top to bottom, and there is water distribution and series connection to fix
The ovum bucket that escapes acts on.Yi Luantong bottom centre (center of circle) is equipped with into water pipe passage port, and pore size is according to depending on tower shoulder column diameter.
Each ease ovum bucket port passes through water inlet pipe, and successively block set is fixed on corresponding tower shoulder, and upper section ease ovum bucket is sequentially embedded lower section ease ovum
In bucket, most upper section ease ovum bucket (setting-out bucket) staving is minimum.Water outlet is arranged at (nearly bucket bottom) above water inlet clamp seat in each bucket
Mouthful.On tower water inlet pipe different size deck be conducive to escape ovum bucket successively be set with or dismounts, and can reach different size escape ovum bucket
Load-bearing effect is consolidated after series connection.
Specific embodiment 2: illustrating present embodiment in conjunction with Fig. 1 to 2, present embodiment and specific embodiment one are not
With point be: the ease ovum bucket 2 be by one 2-1 of ease ovum bucket, ease two 2-2 of ovum bucket, ease three 2-3 of ovum bucket, ease four 2-4 of ovum bucket and
Escape five 2-5 of ovum bucket composition；One 2-1 of ease ovum bucket is placed in ease two 2-2 of ovum bucket, and ease two 2-2 of ovum bucket is placed in ease three 2-3 of ovum bucket
Interior, ease three 2-3 of ovum bucket is placed in ease four 2-4 of ovum bucket, and ease four 2-4 of ovum bucket is placed in ease five 2-5 of ovum bucket.
Other compositions and connection type are same as the specific embodiment one.
Specific embodiment 3: illustrating present embodiment in conjunction with Fig. 1 to 2, present embodiment and specific embodiment one are not
It is with putting；It is set respectively on ease one 2-1 of ovum bucket, ease two 2-2 of ovum bucket, ease three 2-3 of ovum bucket, ease four 2-4 of ovum bucket and ease five 2-5 of ovum bucket
Being equipped with aperture is 4.5mm, 3.5mm, 2.5mm, 1.5mm and 0.5mm ease micropyle 9.
Other compositions and connection type are same as the specific embodiment one.
Specific embodiment 4: illustrating present embodiment in conjunction with Fig. 1 to 2, present embodiment and specific embodiment one are not
Be with point: ease ovum bucket 2 is made of the different ease ovum bucket of multiple internal diameters, and it is different that exhaust outlet 17 is set to multiple internal diameters
It escapes in ovum bucket.
Other compositions and connection type are same as the specific embodiment one.
Specific embodiment 5: illustrating present embodiment in conjunction with Fig. 1 to 2, present embodiment and specific embodiment one are not
Be with point: the aperture of spillway hole 11 is less than the aperture of the ease micropyle 9 of minimum-value aperture.
Other compositions and connection type are same as the specific embodiment one.
Specific embodiment 6: illustrating present embodiment in conjunction with Fig. 1 to 2, present embodiment and specific embodiment one are not
It is with point: the disc structure being open centered on ease ovum bucket deck 10.
Other compositions and connection type are same as the specific embodiment one.
Specific embodiment 7: illustrating present embodiment in conjunction with Fig. 1 to 2, present embodiment and specific embodiment one are not
Be with point: ease 2 upper end of ovum bucket is placed in slot 18, and ease 2 upper end of ovum bucket and the gap of slot 18 are less than 0.2mm.
Other compositions and connection type are same as the specific embodiment one.
Specific embodiment 8: illustrating present embodiment, a kind of field acquisition floatability of present embodiment in conjunction with Fig. 1 to 2
Fish-egg biopsy sample escape ovum suit separator application method, it the following steps are included:
Firstly, the ease ovum bucket 2 of different inner diameters is sequentially placed according to the sequence of internal diameter from large to small；Then, by water-supply-pipe 3
One end sequentially passes through one 6-1 of water delivery pipe through-hole on two 6-2 of water delivery pipe through-hole and ease ovum bucket 2 on lid 4, is placed in holding barrel 1
Bottom, the other end are connected to the water outlet of water butt 5；The ease ovum bucket 2 of different inner diameters is placed on ease ovum bucket deck 10 again, and
And water outlet 7 is distributed in the ease ovum bucket 2 of guarantee different inner diameters；Ease ovum bucket 2 is placed in holding barrel 1；Secondly, by exhaust pipe
In the ease ovum bucket 2 of outlet 17 respectively, the air inlet of exhaust outlet 17 passes through the appendix 14 across gas transmission pipe through-hole 13 respectively
It is connected to the gas outlet of air pump 15；Again, fish-egg sample is put into the smallest ease ovum bucket 2 of internal diameter after will be to be processed；Finally, will
Lid 4 is placed on holding barrel 1, and is connect by buckle 12 with holding barrel 1；The effluent switch for first opening water butt 5, passes through water outlet
7 water flowings into ease ovum bucket 2, then air pump 15 and battery are connected, open air pump 15 to ease 2 gas transmission of ovum bucket, that is, complete described
The use of field acquisition floatability fish-egg biopsy sample suit ease ovum Combined separator.
Specific embodiment 9: illustrating present embodiment in conjunction with Fig. 1 to 2, present embodiment and specific embodiment eight are not
Be with point: the water-supply-pipe 3 and ease 10 material of ovum bucket deck is PVC；The two is linked together by bonding way
's.
Other compositions and connection type are identical as specific embodiment eight.
Specific embodiment 10: illustrating present embodiment in conjunction with Fig. 1 to 2, present embodiment and specific embodiment eight are not
Be with point: the gap of the water-supply-pipe 3 and one 6-1 of water delivery pipe through-hole two and water delivery pipe through-hole is less than 0.2mm, and appendix is logical
Hole 13 and 14 gap of appendix are less than 0.2mm.
Other compositions and connection type are identical as specific embodiment eight.
The content of present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several specific embodiments
The purpose of invention also may be implemented in contract sample.
Beneficial effects of the present invention are verified by following embodiment:
A kind of application method of field acquisition floatability fish-egg biopsy sample ease ovum suit separator of the present embodiment, uses
The separator of specific embodiment one carries out, it the following steps are included:
Firstly, the ease ovum bucket 2 of different inner diameters is sequentially placed according to the sequence of internal diameter from large to small；Then, by water-supply-pipe 3
One end sequentially passes through one 6-1 of water delivery pipe through-hole on two 6-2 of water delivery pipe through-hole and ease ovum bucket 2 on lid 4, is placed in holding barrel 1
Bottom, the other end are connected to the water outlet of water butt 5；Ease ovum bucket deck 10 water-supply-pipe 3 is mounted respectively to again to support in difference
The ease ovum bucket 2 of diameter, and guarantee to be distributed with water outlet 7 in the ease ovum buckets 2 of different inner diameters；Ease ovum bucket 2 is placed in holding barrel 1
It is interior；Secondly, exhaust outlet 12 is placed in the smallest ease ovum bucket 2 of internal diameter, the air inlet of exhaust outlet 12 is defeated by passing through
The appendix 14 of tracheae through-hole 13 is connected to the gas outlet of air pump 15；Again, fish-egg sample is put into internal diameter most after will be to be processed
In small ease ovum bucket 2；Finally, lid 4 is placed on holding barrel 1, and it is connect by buckle 12 with holding barrel 1；And by holding barrel 1
It is inverted, first opens the effluent switch of water butt 5, connect by the water flowing into ease ovum bucket 2 of water outlet 7, then by air pump 15 with battery
It is logical, air pump 15 is opened to ease 2 gas transmission of ovum bucket, that is, completes the field acquisition floatability fish-egg biopsy sample suit ease ovum combination
The use of formula separator.
It checks that every batch of sample screening effect sets the separating treatment time, each bucket fish-egg is collected respectively after separation and is packed into sample
Living body preservation is carried out in this preservation bottle or Portable fish tank, is immediately available for sampling observation and is taken pictures, measures, classifying, counting, comparing
Processing and data preliminary analysis, and can have according to analysis result to the programmes of work such as field samples acquisition and resource investigation monitoring
Effect property makes corresponding judgement.If can fish-egg or prelarva living body be saved or temporarily be supported and continue development growth, real-time tracking vivo observation
And analysis, and the preservation of transfer strange land or fixed sample in time, increase collecting sample scientific research purposes.
Claims (10)
1. a kind of field acquisition floatability fish-egg biopsy sample ease ovum is set with separator, it is characterised in that it include holding barrel (1),
It escapes ovum bucket (2), water-supply-pipe (3), lid (4), water butt (5), appendix (14) and air pump (15)；
The ease ovum bucket (2) is made of the different ease ovum bucket of multiple internal diameters, and the ease ovum bucket of relatively small internal diameter is placed in relatively large
Ease ovum bucket in；Water delivery pipe through-hole one (6-1) and water delivery pipe through-hole two are offered respectively on ease ovum bucket (2) bottom and lid (4)
(6-2), the water-supply-pipe that water-supply-pipe (3) one end sequentially passes through on water delivery pipe through-hole two (6-2) and ease ovum bucket (2) on lid (4) are logical
Hole one (6-1) is placed in holding barrel (1) bottom；It is provided on water-supply-pipe (3) multiple water outlets (7), the water inlet of water outlet (7)
Mouth is connected to the water outlet of water butt (5) respectively by water-supply-pipe (3), and water outlet (7) is located in ease ovum bucket (2)；Positioned at Sheng
Water-supply-pipe (3) in sample bucket (1) is more piece tower structure, and lower end is placed in holding barrel (1) bottom；The water-supply-pipe (3) of tower structure
Internal diameter increases section by section from top to bottom；Every section water-supply-pipe (3) and adjacent upper section water-supply-pipe (3) form tower shoulder；Tower shoulder is provided with
It escapes ovum bucket deck (10), ease ovum bucket (2) is placed in ease ovum bucket deck (10)；
Ease ovum bucket (2) side wall of different inner diameters is equipped with diversion trench (8), and ease micropyle (9), and phase are provided in diversion trench (8)
The aperture for the ease micropyle (9) being arranged on the ease ovum bucket (2) big to internal diameter is greater than the ease being arranged on the opposite small ease ovum bucket (2) of internal diameter
The aperture of micropyle (9)；Lid (4) is placed on holding barrel (1), is provided with spillway hole (16) between holding barrel (1) and lid (4), is covered (4)
It is connected on holding barrel (1) by buckle (12)；Lid (4) is provided with slot (18), and ease ovum bucket (2) upper end is placed in slot (18)；
Wherein, the aperture of spillway hole (11) and relief hole (16) is respectively less than rear fish-egg Sample diameter to be processed；
Exhaust outlet (17) is set in ease ovum bucket (2), offers gas transmission pipe through-hole (13), exhaust outlet on lid (4)
(17) air inlet is connected to by passing through the appendix (14) of gas transmission pipe through-hole (13) with the gas outlet of air pump (15).
2. a kind of field acquisition floatability fish-egg biopsy sample ease ovum according to claim 1 is set with separator, feature
It is that the ease ovum bucket (2) is by ease ovum bucket one (2-1), ease ovum bucket two (2-2), ease ovum bucket three (2-3), ease four (2- of ovum bucket
4) it is formed with ease ovum bucket five (2-5)；The ease ovum bucket one (2-1) is placed in ease ovum bucket two (2-2), and ease ovum bucket two (2-2) is set
In in ease ovum bucket three (2-3), ease ovum bucket three (2-3) is placed in ease ovum bucket four (2-4), and ease ovum bucket four (2-4) is placed in ease ovum bucket five
In (2-5).
3. a kind of field acquisition floatability fish-egg biopsy sample ease ovum according to claim 2 is set with separator, feature
It is to escape ovum bucket one (2-1), in ease ovum bucket two (2-2), ease ovum bucket three (2-3), ease ovum bucket four (2-4) and ease ovum bucket five (2-5)
Being respectively arranged with aperture is that 4.5mm, 3.5mm, 2.5mm, 1.5mm and 0.5mm escape micropyle (9).
4. a kind of field acquisition floatability fish-egg biopsy sample ease ovum according to claim 1 is set with separator, feature
It is that ease ovum bucket (2) is made of the different ease ovum bucket of multiple internal diameters, it is different that exhaust outlet (17) is set to multiple internal diameters
It escapes in ovum bucket.
5. a kind of field acquisition floatability fish-egg biopsy sample ease ovum according to claim 1 is set with separator, feature
It is that the aperture of spillway hole (11) is less than the aperture of the ease micropyle (9) of minimum-value aperture.
6. a kind of field acquisition floatability fish-egg biopsy sample ease ovum according to claim 1 is set with separator, feature
It is the disc structure being open centered on ease ovum bucket deck (10).
7. a kind of field acquisition floatability fish-egg biopsy sample ease ovum according to claim 1 is set with separator, feature
It is that ease ovum bucket (2) upper end is placed in slot (18), and the gap of ease ovum bucket (2) upper end and slot (18) is less than 0.2mm.
8. a kind of user of field acquisition floatability fish-egg biopsy sample ease ovum suit separator as described in claim 1
Method, it is characterised in that it the following steps are included:
Firstly, the ease ovum bucket (2) of different inner diameters is sequentially placed according to the sequence of internal diameter from large to small；Then, by water-supply-pipe (3)
One end sequentially passes through the water delivery pipe through-hole one (6-1) on water delivery pipe through-hole two (6-2) and ease ovum bucket (2) on lid (4), is placed in
Holding barrel (1) bottom, the other end are connected to the water outlet of water butt (5)；The ease ovum bucket (2) of different inner diameters is placed in ease ovum bucket again
On deck (10), and guarantees that the ease ovum bucket (2) of different inner diameters is interior and be distributed with water outlet (7)；Ease ovum bucket (2) is placed in Sheng sample
In bucket (1)；Secondly, the air inlet of exhaust outlet (17) passes through respectively by the ease ovum bucket (2) of exhaust outlet (17) respectively
Appendix (14) across gas transmission pipe through-hole (13) is connected to the gas outlet of air pump (15)；Again, fish-egg sample after will be to be processed
It is put into the smallest ease ovum bucket (2) of internal diameter；Finally, lid (4) is placed on holding barrel (1), and by buckle (12) and contain sample
Bucket (1) connection；The effluent switch for first opening water butt (5), by water outlet (7) to ease ovum bucket (2) interior water flowing, then by air pump
(15) it is connected with battery, opens air pump (15) to ease ovum bucket (2) gas transmission, that is, it is living to complete the field acquisition floatability fish-egg
The use of body sample suit ease ovum Combined separator.
9. a kind of user of field acquisition floatability fish-egg biopsy sample ease ovum suit separator according to claim 8
Method, it is characterised in that the water-supply-pipe (3) and ease ovum bucket deck (10) material is PVC；The two is connected by bonding way
It is connected together.
10. a kind of use of field acquisition floatability fish-egg biopsy sample ease ovum suit separator according to claim 9
Method, it is characterised in that the water-supply-pipe (3) and the gap of water delivery pipe through-hole two and water delivery pipe through-hole one (6-1) is less than
0.2mm, gas transmission pipe through-hole (13) and appendix (14) gap are less than 0.2mm.
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| CN201910232790.9A CN109757413B (en) | 2019-03-26 | 2019-03-26 | Sleeved separator for field collection of floating roe living body sample egg escape and separation method |
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| CN201910232790.9A CN109757413B (en) | 2019-03-26 | 2019-03-26 | Sleeved separator for field collection of floating roe living body sample egg escape and separation method |
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| CN113179979A (en) * | 2021-04-01 | 2021-07-30 | 广西壮族自治区水产技术推广站 | Method for collecting fertilized eggs of finless eel |
| CN114128652A (en) * | 2021-12-02 | 2022-03-04 | 大连海洋大学 | Black spot medaka embryo hatching device and method |
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| CN114128652A (en) * | 2021-12-02 | 2022-03-04 | 大连海洋大学 | Black spot medaka embryo hatching device and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109757413B (en) | 2021-07-27 |
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