CN109757413B - Sleeved separator for field collection of floating roe living body sample egg escape and separation method - Google Patents
Sleeved separator for field collection of floating roe living body sample egg escape and separation method Download PDFInfo
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- CN109757413B CN109757413B CN201910232790.9A CN201910232790A CN109757413B CN 109757413 B CN109757413 B CN 109757413B CN 201910232790 A CN201910232790 A CN 201910232790A CN 109757413 B CN109757413 B CN 109757413B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a sleeved separator and a separation method for collecting floating roe living body samples in the field, relates to a device and a method for separating the fish roe samples, and aims to solve the problem of nondestructive or micro-damage separation of the fish roe living body collected in the field.
Description
Technical Field
The invention relates to a fish egg sample living body separation device and a separation method.
Background
In research studies on fishery resources and fish spawning sites, it is necessary to collect fish eggs and fish larvae samples in addition to fish samples of different specifications and ages to estimate the spawning site location, early resource occurrence, resource proliferation, spawning scale of various fishes, and the like. Most of fishes are fertilized in vitro, that is, under the condition that the ecological environment condition of a spawning site meets the breeding condition, male and female adult fishes chase after each other under the action of the breeding physiological hormone, mature eggs and sperms are removed from the body to finish fertilization, fertilized eggs are hatched in water, and the individual development process is started. The morphological structure properties of mature eggs and fertilized eggs of different fishes are different. According to the floating property, the eggs are divided into drifting eggs, floating eggs, sinking eggs and viscid eggs, and meanwhile, the eggs are distinguished by size and specification, mature eggs are spherical, and the diameters of the mature eggs are generally different from 0.5 to 6.0 mm. For floating eggs and floating eggs (floating eggs for short), because the eggs and the fries do not have swimming capability, namely the eggs and the fries can only float and descend or diffuse along the water flow from a breeding area of a spawning ground in a river or lake reservoir, the rising water flow enables the egg membranes to continuously absorb water and expand in the floating process, the egg diameter is increased, embryos are gradually hatched and developed, finally the eggs are broken into membranes and grow to the fries which are hatched initially, and the activities of active swimming, bait-pulling and the like can not be carried out until fins and swim bladders exist. The fish egg is spherical in the living state in water, and the size of the egg diameter is an important index for collecting and classifying and identifying fish egg samples. The special standard collecting net for roe and fry survey is used for collecting roe and fry hatched fry in the direction opposite to water flow, and can collect various roe and fry with the egg diameter over 0.5mm in water. And the acquisition net is used for positioning and acquiring samples at regular time during investigation. In the breeding season of fishes in rivers and lakes, the breeding season is mostly concentrated in spring and summer, fish eggs and fish fries of various fishes often occur simultaneously, collected samples are mixed samples of various fishes, and suspended matters or floating objects in water are also included. In the investigation field, samples and sundries carried by the samples are fished out from a collecting net and placed into a sample container, the sundries visible to the naked eye are picked out and discarded, and then primary treatment such as roe and fry sample shape observation, sampling counting and the like is carried out, but the work such as living body separation, measurement, classification, counting, statistical treatment and the like on various fish roes is difficult to carry out on the field. Meanwhile, as the egg membranes of the roes are thin after swelling and easy to break, the roes are easy to damage by manual shoveling and scooping and batch classification treatment, time and labor are wasted, and the roes are easy to die after leaving water. The field investigation field conditions are simple and crude, the samples cannot be stored after living body separation and fine treatment, at present, only part of mixed samples (formalin or alcohol fixing solution) can be manually picked up and extracted for fixing, and the mixed samples are taken back to a laboratory for further classification identification and statistics, while the fixed roe samples are dead, the color of roes is faded, the shape of roes is changed, the diameter of roes is changed, and the information data of living body observation classification identification is lost.
Disclosure of Invention
The invention aims to solve the problem of nondestructive or micro-damage separation of a fish egg living body collected in the field, and provides a sleeved separator and a separation method for floating fish egg living body sample egg escaping collected in the field.
The invention relates to a suit separator for escaping eggs of a field collection floating roe living body sample, which comprises a sample containing barrel, an egg escaping barrel, a water delivery pipe, a cover, a water storage barrel, a gas delivery pipe and an air pump, wherein the sample containing barrel is connected with the water delivery pipe; the egg escaping barrel consists of a plurality of egg escaping barrels with different inner diameters, and the egg escaping barrel with a relatively small inner diameter is arranged in the egg escaping barrel with a relatively large inner diameter; the bottom of the egg escape barrel and the cover are respectively provided with a water delivery pipe through hole I and a water delivery pipe through hole II, one end of the water delivery pipe sequentially penetrates through the water delivery pipe through hole II on the cover and the water delivery pipe through hole I on the egg escape barrel and is arranged at the bottom of the sample containing barrel; a plurality of water outlets are arranged on the water conveying pipe, water inlets of the water outlets are respectively communicated with water outlets of the water storage barrel through the water conveying pipe, and the water outlets are positioned in the egg escaping barrel; the water delivery pipe positioned in the sample holding barrel is of a multi-section tower type structure, and the lower end of the water delivery pipe is arranged at the bottom of the sample holding barrel; the inner diameter of the water delivery pipe of the tower structure is gradually increased from top to bottom; each section of water conveying pipe and the adjacent upper section of water conveying pipe form a tower shoulder; the tower shoulder is provided with an egg releasing barrel clamping seat, and the egg releasing barrel is arranged on the egg releasing barrel clamping seat;
the tower shoulders with different inner diameters are provided with guide grooves, egg escape holes are formed in the guide grooves, and the aperture of the egg escape hole formed in the egg escape barrel with the larger inner diameter is larger than that of the egg escape hole formed in the egg escape barrel with the smaller inner diameter; an overflow hole is arranged between the sample containing barrel and the cover; the cover covers the sample containing barrel and is clamped on the sample containing barrel through a buckle; the cover is provided with a plurality of grooves, and the upper end of the egg escape barrel is arranged in the grooves;
the diameters of the overflow hole and the pressure relief hole are smaller than the diameter of the roe sample to be treated;
the outlet of the exhaust pipe is arranged in the egg escaping barrel, the cover is provided with a gas pipe through hole, and the gas inlet of the outlet of the exhaust pipe is communicated with the gas outlet of the gas pump through the gas pipe penetrating through the gas pipe through hole.
The invention discloses a use method of a sleeved separator for escaping eggs of a floating roe living body sample collected in the field, which comprises the following steps:
firstly, placing egg escaping barrels with different inner diameters in sequence according to the order of the inner diameters from large to small; then, one end of the water delivery pipe sequentially penetrates through a second water delivery pipe through hole on the cover and a first water delivery pipe through hole on the egg escape barrel and is arranged at the bottom of the sample containing barrel, and the other end of the water delivery pipe is communicated with a water outlet of the water storage barrel; then, placing the egg escaping barrels with different inner diameters on the egg escaping barrel clamping seats, and ensuring that water outlets are uniformly distributed in the egg escaping barrels with different inner diameters; placing the egg overflowing barrel in the sample containing barrel; secondly, an outlet of the exhaust pipe is arranged in the egg escape barrel with the smallest inner diameter, and an air inlet of the outlet of the exhaust pipe is communicated with an air outlet of the air pump through an air conveying pipe penetrating through an air conveying pipe through hole; thirdly, putting the roe sample to be treated into an egg escape barrel with the smallest inner diameter; finally, covering the sample containing barrel with a cover, and connecting the sample containing barrel with the cover through a buckle; the water outlet switch of the water storage barrel is firstly opened, water is introduced into the egg escaping barrel through the water outlet, the air pump is connected with the storage battery when needed, and the air pump is opened to deliver air to the egg escaping barrel, so that the assembly of the egg escaping combined separator for collecting the floating fish egg living body sample in the field is completed.
The device can separate fish egg samples by a method of enabling water to flow or rotate or float in a sample container (a separator) on an investigation site to enable fish eggs to be freely removed from escape holes in floating and rotating according to floatability and particle size specifications of the fish eggs, so that sample processing units with different particle sizes can be separated from fish egg sample groups, and the collected original mixed samples can be separated and classified.
The device is a combined separator of a sleeved egg-escaping barrel, the sample containing barrel and the egg-escaping barrel are made of non-inductive steel or plastic materials, the whole appearance of the separator is cylindrical, the sample containing barrel is covered by a cover, and the cover is clamped on the outer wall of the sample containing barrel by a buckle for further fixation. A gap is formed between the sample containing barrel and the cover, namely the overflow hole, and is used for discharging pressure formed after the sample containing barrel is filled with water, and also for discharging water to form flowing water after the sample containing barrel is filled with water, the overflow hole can play a role in dispersing the direction of water flow, can drive roes to be uniformly distributed at the bottom of the roe dispersing barrel, and is convenient for separating the roes. The egg escaping barrels are all U-shaped barrels, and the side walls of the egg escaping barrels are streamline to facilitate the flow of a water body; the egg releasing barrel is composed of a plurality of egg releasing barrels with different inner diameters, and the egg releasing barrels with small inner diameters are arranged in the egg releasing barrels with large inner diameters in the order of the inner diameters from small to large; the bottom of each egg overflowing barrel is provided with a water conveying pipe through hole I, the water conveying pipe penetrates through the water conveying pipe through hole I and is arranged in the egg overflowing barrels with different inner diameters, and the egg overflowing barrel clamping seat is arranged on the water conveying pipe and is used for supporting the egg overflowing barrels with different inner diameters, so that certain space is formed between the egg overflowing barrels with different inner diameters, and fish eggs with different sizes can be stored. The tower shoulder is provided with a diversion trench, and the escape holes are distributed in the diversion trench. The bottom of each egg escape barrel is distributed with a plurality of escape holes with the same aperture (according to the distribution data of the egg diameters of a plurality of fishes, the escape holes of the egg escape barrels are 4.5mm, 3.5mm, 2.5mm, 1.5mm and 0.5mm from large to small in sequence) and are used for screening the fish eggs with the same particle size. In order to further prevent the roes from gathering near the escape hole and causing blockage, the air blowing device designed by the invention can be used as necessary, the air pump is communicated with the outlet of the exhaust pipe through the air guide pipe, the outlet of the exhaust pipe is arranged in the roe escape barrel for preventing roe samples, and after the air pump is connected with the storage battery, air is discharged to the roe escape barrel to generate bubbles, so that the action similar to field water body stirring and rolling is formed, and the roes can be separated. The clearance between the water delivery pipe and the water delivery pipe through hole II and the water delivery pipe through hole I is smaller than the minimum diameter of the roe to be separated, and is preferably about 0.1 mm. The aperture of the overflow hole and the aperture of the pressure relief hole are both smaller than the minimum diameter of the roe sample to be treated. The height of the water storage barrel is higher than that of the egg escaping barrel, and a certain height difference is required, so that the water in the water storage barrel can flow into the egg escaping barrel. The water used can be collected from the water area from which the roe sample is to be isolated.
The invention has the following beneficial effects:
the separator device and the separation processing method solve the problem of nondestructive or micro-damage separation of the fish egg living body collected in the field, provide samples for the living body observation research on the investigation site, have a combined type sleeved separation design, are time-saving and labor-saving, can be assembled in a split manner, are convenient to carry, and are suitable for the shore-side powerless operation of the field river bank or lake bank and the like. After the fish egg living body is separated, the samples can be accurately classified, and the subsequent biological and resource proliferation protection and other related researches can be carried out. The combined type sleeve separation device provides a sample for in-vivo observation research on an investigation site, is time-saving and labor-saving in combined type sleeve separation design, can be assembled in a split manner, is convenient to carry, and is suitable for shore-side powerless operation such as field river banks or lake banks.
Drawings
FIG. 1 is a schematic diagram of a separator according to the present invention;
fig. 2 is a sectional view of a cover structure in the separator of the present invention.
Detailed Description
The first embodiment is as follows: the embodiment is described with reference to fig. 1 to 2, and the device for sleeving and separating eggs of a live sample of the floatage eggs collected in the field comprises a sample containing barrel 1, an egg escaping barrel 2, a water conveying pipe 3, a cover 4, a water storage barrel 5, a gas conveying pipe 14 and a gas pump 15;
the egg escaping barrel 2 consists of a plurality of egg escaping barrels with different inner diameters, and the egg escaping barrel with a relatively small inner diameter is arranged in the egg escaping barrel with a relatively large inner diameter; the bottom of the egg escaping barrel 2 and the cover 4 are respectively provided with a water delivery pipe through hole I6-1 and a water delivery pipe through hole II 6-2, one end of the water delivery pipe 3 sequentially passes through the water delivery pipe through hole II 6-2 on the cover 4 and the water delivery pipe through hole I6-1 on the egg escaping barrel 2 and is arranged at the bottom of the sample containing barrel 1; a plurality of water outlets 7 are arranged on the water conveying pipe 3, the water inlets of the water outlets 7 are respectively communicated with the water outlets of the water storage barrel 5 through the water conveying pipe, and the water outlets 7 are positioned in the ovum escaping barrel 2; the water delivery pipe 3 positioned in the sample holding barrel 1 is of a multi-section tower type structure, and the lower end of the water delivery pipe is arranged at the bottom of the sample holding barrel 1; the inner diameter of the water delivery pipe 3 of the tower structure is gradually increased from top to bottom; each section of the water conveying pipe 3 and the adjacent upper section of the water conveying pipe 3 form a tower shoulder; the tower shoulder is provided with an egg escaping barrel clamping seat 10, and the egg escaping barrel 2 is arranged on the egg escaping barrel clamping seat 10;
the side walls of the egg escaping barrels 2 with different inner diameters are provided with guide grooves 8, egg escaping holes 9 are arranged in the guide grooves 8, and the aperture of the egg escaping hole 9 arranged on the egg escaping barrel 2 with the larger inner diameter is larger than that of the egg escaping hole 9 arranged on the egg escaping barrel 2 with the smaller inner diameter; an overflow hole 16 is arranged between the sample containing barrel 1 and the cover 4; the cover 4 covers the sample containing barrel 1 and is clamped on the sample containing barrel 1 through a buckle 12; the cover 4 is provided with a plurality of grooves 18, and the upper end of the ovum escaping barrel 2 is arranged in the grooves 18;
the diameters of the overflow hole 16 and the pressure relief hole are smaller than the diameter of the roe sample to be treated;
the exhaust pipe outlet 17 is arranged in the egg escape barrel 2, the cover 4 is provided with an air pipe through hole 13, and the air inlet of the exhaust pipe outlet 17 is communicated with the air outlet of the air pump 15 through an air pipe 14 penetrating through the air pipe through hole 13.
Adhesive tapes are arranged among the first water pipe through hole 6-1, the second water pipe through hole 6-2, the through hole 13, the water pipe 3 and the air conveying pipe 14, and the sealing effect is achieved.
The water outlet 7 of the egg escape barrel is in threaded connection with the water conveying pipe 3, so that the egg escape barrel is convenient to disassemble, the egg escape barrels with different inner diameters are convenient to separate from the water conveying pipe 3 after the water outlet 7 is removed, the exhaust pipe outlet 17 is also in threaded connection with the air conveying pipe 14, so that the egg escape barrel is convenient to disassemble, and the egg escape barrels with different inner diameters are convenient to separate from the air conveying pipe 14 after the exhaust pipe outlet 17.
The water delivery pipe 3 of the embodiment is positioned in the center of the sample containing barrel 1, the water delivery pipe 3 positioned in the sample containing barrel 1 is a multi-section tower-type tubular body, each section is provided with a plurality of water outlets and a tower shoulder, and the tower shoulders are gradually enlarged from top to bottom and have the functions of water distribution and fixing egg escape barrels in series connection. The center (circle center) of the bottom of the ovum escape barrel is provided with a water inlet pipe port, and the aperture size is determined according to the diameter of the tower shoulder column. Each egg leakage barrel opening penetrates through the water inlet pipe and is sequentially fixed on the corresponding tower shoulder, the upper egg leakage barrel is sequentially embedded into the lower egg leakage barrel, and the barrel body of the uppermost egg leakage barrel (sample placement barrel) is the smallest. The upper part (close to the bottom of the barrel) of the clamping seat of the water inlet pipe in each barrel is provided with a water outlet. The clamping seats with different sizes on the tower-type water inlet pipe are beneficial to sleeving or dismounting the egg-escaping barrels in sequence, and the stable bearing effect of the egg-escaping barrels with different sizes after being connected in series can be achieved.
The second embodiment is as follows: the present embodiment will be described with reference to fig. 1 to 2, and the present embodiment is different from the specific embodiment in that: the egg overflowing barrel 2 consists of a first egg overflowing barrel 2-1, a second egg overflowing barrel 2-2, a third egg overflowing barrel 2-3, a fourth egg overflowing barrel 2-4 and a fifth egg overflowing barrel 2-5; the first egg overflowing barrel 2-1 is arranged in the second egg overflowing barrel 2-2, the second egg overflowing barrel 2-2 is arranged in the third egg overflowing barrel 2-3, the third egg overflowing barrel 2-3 is arranged in the fourth egg overflowing barrel 2-4, and the fourth egg overflowing barrel 2-4 is arranged in the fifth egg overflowing barrel 2-5.
Other components and connection modes are the same as those of the first embodiment.
The third concrete implementation mode: the present embodiment is described with reference to fig. 1 to 2, and the present embodiment is different from the present embodiment in a point of difference; the first egg escaping barrel 2-1, the second egg escaping barrel 2-2, the third egg escaping barrel 2-3, the fourth egg escaping barrel 2-4 and the fifth egg escaping barrel 2-5 are respectively provided with egg escaping holes 9 with the aperture of 4.5mm, 3.5mm, 2.5mm, 1.5mm and 0.5 mm.
Other components and connection modes are the same as those of the first embodiment.
The fourth concrete implementation mode: the present embodiment will be described with reference to fig. 1 to 2, and the present embodiment is different from the specific embodiment in that: the egg escaping barrel 2 is composed of a plurality of egg escaping barrels with different inner diameters, and the outlet 17 of the exhaust pipe is arranged in the egg escaping barrels with different inner diameters.
Other components and connection modes are the same as those of the first embodiment.
The fifth concrete implementation mode: the present embodiment will be described with reference to fig. 1 to 2, and the present embodiment is different from the specific embodiment in that: the aperture of the overflow hole 16 is smaller than that of the egg escape hole 9 with the smallest aperture.
Other components and connection modes are the same as those of the first embodiment.
The sixth specific implementation mode: the present embodiment will be described with reference to fig. 1 to 2, and the present embodiment is different from the specific embodiment in that: the egg-escaping barrel clamping seat 10 is a disc structure with an opening in the center.
Other components and connection modes are the same as those of the first embodiment.
The seventh embodiment: the present embodiment will be described with reference to fig. 1 to 2, and the present embodiment is different from the specific embodiment in that: the upper end of the egg-escaping barrel 2 is arranged in the groove 18, and the clearance between the upper end of the egg-escaping barrel 2 and the groove 18 is less than 0.2 mm.
Other components and connection modes are the same as those of the first embodiment.
The specific implementation mode is eight: the embodiment is described with reference to fig. 1 to 2, and the application method of the suit separator for collecting floating roe living body samples in field by using the roe escaping suit separator comprises the following steps:
firstly, placing egg escaping barrels 2 with different inner diameters in sequence according to the order of the inner diameters from large to small; then, one end of the water delivery pipe 3 sequentially passes through a water delivery pipe through hole II 6-2 on the cover 4 and a water delivery pipe through hole I6-1 on the egg escape barrel 2 and is arranged at the bottom of the sample containing barrel 1, and the other end of the water delivery pipe is communicated with a water outlet of the water storage barrel 5; then, the egg releasing barrels 2 with different inner diameters are arranged on the egg releasing barrel clamping seats 10, and water outlets 7 are distributed in the egg releasing barrels 2 with different inner diameters; placing the ovum overflow barrel 2 in the sample containing barrel 1; secondly, respectively enabling the air outlets 17 of the exhaust pipes to be respectively communicated with the air outlets of the air pump 15 through the air pipes 14 penetrating through the air pipe through holes 13 in the egg escape barrel 2; thirdly, putting the roe sample to be treated into the roe escaping barrel 2 with the smallest inner diameter; finally, covering the sample containing barrel 1 with a cover 4, and connecting the sample containing barrel 1 with a buckle 12; the water outlet switch of the water storage barrel 5 is firstly opened, water is introduced into the egg escaping barrel 2 through the water outlet 7, the air pump 15 is then communicated with the storage battery, the air pump 15 is opened to deliver air to the egg escaping barrel 2, and the assembly type separator for collecting the floating roe living body sample in the field is used.
The specific implementation method nine: the present embodiment is described with reference to fig. 1 to 2, and is different from the specific embodiment in eight points: the material of the water delivery pipe 3 and the material of the egg escaping barrel clamping seat 10 are both PVC; the two are connected together by means of adhesive.
The other components and connection modes are the same as those of the eighth embodiment.
The detailed implementation mode is ten: the present embodiment is described with reference to fig. 1 to 2, and is different from the specific embodiment in eight points: the clearance between the water delivery pipe 3 and the water delivery pipe through hole II and the clearance between the water delivery pipe through hole I6-1 are less than 0.2mm, and the clearance between the air delivery pipe through hole 13 and the air delivery pipe 14 is less than 0.2 mm.
The other components and connection modes are the same as those of the eighth embodiment.
The invention is not limited to the above embodiments, and one or a combination of several embodiments may also achieve the object of the invention.
The beneficial effects of the present invention are demonstrated by the following examples:
the use method of the sleeved separator for field collection of floating roe living body samples by egg escaping is carried out by adopting the separator of the first embodiment, and comprises the following steps:
firstly, placing egg escaping barrels 2 with different inner diameters in sequence according to the order of the inner diameters from large to small; then, one end of the water delivery pipe 3 sequentially passes through a water delivery pipe through hole II 6-2 on the cover 4 and a water delivery pipe through hole I6-1 on the egg escape barrel 2 and is arranged at the bottom of the sample containing barrel 1, and the other end of the water delivery pipe is communicated with a water outlet of the water storage barrel 5; then the clamping seats 10 of the egg escaping barrels are respectively arranged on the water conveying pipe 3 to support the egg escaping barrels 2 with different inner diameters, and water outlets 7 are distributed in the egg escaping barrels 2 with different inner diameters; placing the ovum overflow barrel 2 in the sample containing barrel 1; secondly, the exhaust pipe outlet 12 is arranged in the egg escape barrel 2 with the smallest inner diameter, and the air inlet of the exhaust pipe outlet 12 is communicated with the air outlet of the air pump 15 through the air pipe 14 penetrating through the air pipe through hole 13; thirdly, putting the roe sample to be treated into the roe escaping barrel 2 with the smallest inner diameter; finally, covering the sample containing barrel 1 with a cover 4, and connecting the sample containing barrel 1 with a buckle 12; and inverting the sample containing barrel 1, turning on a water outlet switch of the water storage barrel 5, introducing water into the egg escape barrel 2 through the water outlet 7, switching on the air pump 15 and the storage battery, and turning on the air pump 15 to convey air to the egg escape barrel 2, so that the use of the floating fish egg living body sample sleeve egg escape combined separator for field collection is completed.
The screening effect of each batch of samples is checked, the separation processing time is set, after separation, all barrels of fish eggs are respectively collected and put into a sample storage bottle or a portable fish tank for living body storage, the living body storage is immediately used for sampling, observing, photographing, measuring, classifying, counting, comparing and other processing and data preliminary analysis, and corresponding judgment can be made on the effectiveness of working schemes such as field sample collection, resource investigation monitoring and the like according to the analysis result. If the living fish eggs or the larval fish can be preserved or temporarily cultured to continue to grow, the living observation and analysis are tracked in real time, and the samples are timely transferred to be preserved or fixed in different places, so that the scientific research application of the collected samples is increased.
Claims (9)
1. A use method of a suit separator for escaping eggs from a floating roe living body sample collected in the field is characterized by comprising the following steps:
firstly, placing egg escaping barrels (2) with different inner diameters in sequence according to the sequence that the inner diameters are from large to small; then, one end of the water delivery pipe (3) sequentially penetrates through a water delivery pipe through hole II (6-2) on the cover (4) and a water delivery pipe through hole I (6-1) on the egg escape barrel (2) and is placed at the bottom of the sample containing barrel (1), and the other end of the water delivery pipe (3) is communicated with a water outlet of the water storage barrel (5); then, the egg releasing barrels (2) with different inner diameters are placed on the egg releasing barrel clamping seats (10), and water outlets (7) are distributed in the egg releasing barrels (2) with different inner diameters; placing the ovum overflow barrel (2) in the sample containing barrel (1); secondly, respectively communicating the air inlets of the outlets (17) of the exhaust pipes in the egg escape barrel (2) and the air outlets of the air pump (15) through the air conveying pipes (14) penetrating through the through holes (13) of the air conveying pipes; thirdly, putting the roe sample to be treated into the roe escaping barrel (2) with the smallest inner diameter; finally, covering the sample containing barrel (1) with a cover (4), and connecting the sample containing barrel (1) with the cover through a buckle (12); firstly, turning on a water outlet switch of the water storage barrel (5), introducing water into the egg escape barrel (2) through the water outlet (7), then switching on the air pump (15) and the storage battery, and turning on the air pump (15) to convey air to the egg escape barrel (2), thus completing the use of the floating fish egg living body sample sleeve egg escape combined separator for field collection;
the field floating roe living body sample egg escape sleeve separator comprises a sample containing barrel (1), an egg escape barrel (2), a water delivery pipe (3), a cover (4), a water storage barrel (5), a gas delivery pipe (14) and a gas pump (15);
the egg escaping barrel (2) consists of a plurality of egg escaping barrels with different inner diameters, and the egg escaping barrel with a relatively small inner diameter is arranged in the egg escaping barrel with a relatively large inner diameter; the bottom of the egg escaping barrel (2) and the cover (4) are respectively provided with a water delivery pipe through hole I (6-1) and a water delivery pipe through hole II (6-2), one end of the water delivery pipe (3) sequentially penetrates through the water delivery pipe through hole II (6-2) on the cover (4) and the water delivery pipe through hole I (6-1) on the egg escaping barrel (2) and is arranged at the bottom of the sample accommodating barrel (1); a plurality of water outlets (7) are arranged on the water conveying pipe (3), the water inlets of the water outlets (7) are respectively communicated with the water outlets of the water storage barrel (5) through the water conveying pipe (3), and the water outlets (7) are positioned in the egg escaping barrel (2); the water delivery pipe (3) positioned in the sample holding barrel (1) is of a multi-section tower type structure, and the lower end of the water delivery pipe is arranged at the bottom of the sample holding barrel (1); the inner diameter of the water delivery pipe (3) of the tower structure is gradually increased from top to bottom; each section of the water pipe (3) and the adjacent upper section of the water pipe (3) form a tower shoulder; the tower shoulder is provided with an ovum escaping barrel clamping seat (10), and the ovum escaping barrel (2) is arranged on the ovum escaping barrel clamping seat (10);
the side walls of the egg overflowing barrels (2) with different inner diameters are provided with guide grooves (8), egg overflowing holes (9) are formed in the guide grooves (8), and the aperture of the egg overflowing hole (9) formed in the egg overflowing barrel (2) with the larger inner diameter is larger than that of the egg overflowing hole (9) formed in the egg overflowing barrel (2) with the smaller inner diameter; the cover (4) covers the sample containing barrel (1), an overflow hole (16) is arranged between the sample containing barrel (1) and the cover (4), and the cover (4) is clamped on the sample containing barrel (1) through a buckle (12); the cover (4) is provided with a groove (18), and the upper end of the egg escaping barrel (2) is arranged in the groove (18);
the diameters of the overflow hole (16) and the pressure relief hole are smaller than the diameter of the roe sample to be treated;
an exhaust pipe outlet (17) is arranged in the egg escape barrel (2), an air conveying pipe through hole (13) is formed in the cover (4), and an air inlet of the exhaust pipe outlet (17) is communicated with an air outlet of the air pump (15) through an air conveying pipe (14) penetrating through the air conveying pipe through hole (13).
2. The use method of the egg escaping kit separator for the field collection of the floating fish egg living body sample according to claim 1, wherein the egg escaping barrel (2) consists of a first egg escaping barrel (2-1), a second egg escaping barrel (2-2), a third egg escaping barrel (2-3), a fourth egg escaping barrel (2-4) and a fifth egg escaping barrel (2-5); the first egg overflowing barrel (2-1) is arranged in the second egg overflowing barrel (2-2), the second egg overflowing barrel (2-2) is arranged in the third egg overflowing barrel (2-3), the third egg overflowing barrel (2-3) is arranged in the fourth egg overflowing barrel (2-4), and the fourth egg overflowing barrel (2-4) is arranged in the fifth egg overflowing barrel (2-5).
3. The use method of the egg escaping set separator for the field collection of the floating fish egg living body sample according to claim 2, wherein egg escaping holes (9) with the aperture of 4.5mm, 3.5mm, 2.5mm, 1.5mm and 0.5mm are respectively formed in the egg escaping barrel I (2-1), the egg escaping barrel II (2-2), the egg escaping barrel III (2-3), the egg escaping barrel IV (2-4) and the egg escaping barrel V (2-5).
4. The use method of the egg escaping kit separator for the field collection of the floating fish egg living body sample according to claim 1, wherein the egg escaping barrel (2) is composed of a plurality of egg escaping barrels with different inner diameters, and the outlet (17) of the exhaust pipe is arranged in the plurality of egg escaping barrels with different inner diameters.
5. The use method of the egg escaping kit separator for the field collection of the floating fish egg living body sample according to claim 1, wherein the aperture of the overflow hole (16) is smaller than that of the egg escaping hole (9) with the smallest aperture.
6. The use method of the egg escaping kit separator for the field collection of the floating fish egg living body sample according to claim 1, wherein the egg escaping barrel clamping seat (10) is a disk structure with an open center.
7. The use method of the egg escaping kit separator for the field collection of the floating fish egg living body sample according to claim 1, wherein the upper end of the egg escaping barrel (2) is arranged in the groove (18), and the gap between the upper end of the egg escaping barrel (2) and the groove (18) is less than 0.2 mm.
8. The use method of the egg escape kit separator for the field collection of the floating fish egg living body samples according to claim 1, wherein the water conveying pipe (3) and the egg escape barrel clamping seat (10) are made of PVC; the two are connected together by means of adhesive.
9. The use method of the sleeved separator for collecting the floating roe living body samples in the field according to claim 8, wherein the gap between the water pipe (3) and the water pipe through hole II and the water pipe through hole I (6-1) is less than 0.2mm, and the gap between the air pipe through hole (13) and the air pipe (14) is less than 0.2 mm.
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| CN109757413A (en) | 2019-05-17 |
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