CN112229972A - Device for toxicity experiment by using grouper fries - Google Patents
Device for toxicity experiment by using grouper fries Download PDFInfo
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- CN112229972A CN112229972A CN202011093275.6A CN202011093275A CN112229972A CN 112229972 A CN112229972 A CN 112229972A CN 202011093275 A CN202011093275 A CN 202011093275A CN 112229972 A CN112229972 A CN 112229972A
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- grouper
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- 241001417495 Serranidae Species 0.000 title claims abstract description 36
- 231100000820 toxicity test Toxicity 0.000 title claims abstract description 19
- 241000251468 Actinopterygii Species 0.000 claims abstract description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 238000002474 experimental method Methods 0.000 claims abstract description 30
- 238000009395 breeding Methods 0.000 claims abstract description 7
- 230000001488 breeding effect Effects 0.000 claims abstract description 7
- 238000012360 testing method Methods 0.000 claims abstract description 7
- 231100000572 poisoning Toxicity 0.000 claims abstract 4
- 230000000607 poisoning effect Effects 0.000 claims abstract 4
- 239000013535 sea water Substances 0.000 claims description 20
- 239000003440 toxic substance Substances 0.000 claims description 14
- 230000003203 everyday effect Effects 0.000 claims description 12
- 231100000403 acute toxicity Toxicity 0.000 claims description 10
- 230000007059 acute toxicity Effects 0.000 claims description 10
- 231100000167 toxic agent Toxicity 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 6
- 239000006184 cosolvent Substances 0.000 claims description 5
- 231100000636 lethal dose Toxicity 0.000 claims description 5
- 231100000614 poison Toxicity 0.000 claims description 5
- 241001529596 Pontinus kuhlii Species 0.000 claims description 3
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 claims description 3
- 238000011047 acute toxicity test Methods 0.000 claims description 3
- 238000006298 dechlorination reaction Methods 0.000 claims description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 241000357444 Epinephelus coioides Species 0.000 claims 1
- 239000003344 environmental pollutant Substances 0.000 description 5
- 231100000719 pollutant Toxicity 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 231100000111 LD50 Toxicity 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000010196 hermaphroditism Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
Abstract
The invention discloses a device for performing toxicity experiments by using grouper fries, which comprises a grouper fry breeding box, an experiment testing device, a storage box and a drainage tank, wherein the grouper fry breeding box is provided with a breeding fish tank, a first fluorescent lamp, a first temperature controller and a first ventilating device, the water in a control tank at the lower part of the breeding fish tank is discharged into the drainage tank through a first switch valve, the experiment testing device is provided with a poisoning fish tank, a second fluorescent lamp, a second temperature controller and a second ventilating device, the water in a control tank at the lower part of the poisoning fish tank is discharged into the drainage tank through a second switch valve, a box cover which can be drawn on the upper surface of the storage box is convenient for classified storage, and the drainage tank is provided with an outer discharge pipe orifice.
Description
Technical Field
The invention relates to the technical field of environmental monitoring, in particular to a device for performing toxicity experiments by using grouper fries.
Background
Offshore areas are under tremendous environmental pressure as a sink for most land-sourced marine pollutants. Because of the rapid development of economy and the large consumption of resources in China for years, and the lack of a perfect environment management system, the water environment pollution in China is increasingly serious, so that the effective monitoring of the environment is realized, the environmental problem is timely treated, and the method is a necessary measure for the sustainable development in China. Animal toxicity tests provide important evaluation indexes for environmental pollutants and pollution degree by measuring the toxic effect of the pollutants on organisms in a short term or a long term. The grouper is used as a marine dominant species, mostly hermaphrodite, has strong reproductive capacity and easy observation, and is commonly used for animal toxicity experiments. Carry out biological acute toxicity experiment to grouper seedling, be aided with the contrast experiment of cosolvent simultaneously, the physical and chemical conditions of comprehensive consideration experiment water has certain requirement to experimental apparatus, and how to set up experimental apparatus in order to realize the experiment of high-efficient system is the problem that this patent expects to solve.
The invention aims to provide a device for performing toxicity experiments by using grouper fries, which is highly targeted, accurate and efficient, and fully considers the characteristics and conditions of the marine water ecosystem in China, combines experimental requirements and material requirements, and performs specification and adjustment of differences according to different pollutants of different species, aiming at the defects of the conventional toxicity experiment device.
Disclosure of Invention
The invention aims to provide a device for performing toxicity experiments by using grouper fries, which is highly targeted, accurate and efficient, and fully considers the characteristics and conditions of the marine water ecosystem in China, combines experimental requirements and material requirements, and performs specification and adjustment of differences according to different pollutants of different species, aiming at the defects of the conventional toxicity experiment device. The device is more convenient and faster to carry out experiments, saves space, obtains more scientific and reliable toxicity experiment data, and is more suitable for deriving the water quality reference value of the aquatic organisms in the subsequent seawater.
A device for carrying out toxicity experiments by using grouper fries comprises the following parts:
the device includes that grouper fry rearing raises case, experiment testing arrangement, storage tank, water drainage tank, grouper fry rearing raises the case and is provided with raises fish bowl, fluorescent lamp one, temperature controller one, breather one, raise the fish bowl lower part and discharge into water drainage tank through a ooff valve control jar normal water, experiment testing arrangement is provided with contamination fish bowl, fluorescent lamp two, temperature controller two, breather two, contamination fish bowl lower part is gone into water drainage tank through the two control jars of ooff valve normal water, it puts the thing to equip the case classification of being convenient for, the case lid of storage tank upper surface for the pull, water drainage tank is provided with outer discharge pipe mouth.
Furthermore, the exterior of each contaminated fish tank in the device is marked with a toxic substance concentration value.
Furthermore, the illumination time of the fluorescent lamp is 12 hours, and the dark time is 12 hours.
Further, experiment temperature can be adjusted through the temperature controller, oxygen is provided for grouper fries through the ventilation device, and the fish tank is cleaned through the first switch valve and waste water is discharged.
Furthermore, all parts of the experimental device can be detached and flexibly adjusted according to experimental contents and requirements.
An experimental method of a toxicity experimental device by using grouper fries comprises the following steps:
1. rockfish domestication
Collecting tap water, aerating for three days for dechlorination, adding seawater salt to prepare artificial seawater, selecting healthy grouper fries with body length of 5 +/-1 cm, feeding with the artificial seawater for 10 days, emptying intestinal tracts of the fish for one day after domestication is finished, and performing acute toxicity experiments;
2. acute toxicity test
(1) Preparing a toxic substance solution;
(2) preparing artificial seawater, wherein the mass ratio of water to seawater salt is 1: 0.145;
(3) setting four experimental groups and a blank control group, wherein the sizes of all the fish tanks are unified to 27L, the fish tanks are all provided with 2 parallel groups, the experimental groups I, II and III are experimental groups with different concentrations of toxic substances, the experimental group IV is a cosolvent experimental group, the blank group is an artificial seawater experimental group, and the four experimental groups and the blank group have the same conditions except that the water quality is different;
(4) putting 20 grouper fries selected in the step 1 into each fish tank to perform an acute toxicity experiment, wherein during the acute toxicity experiment, half of water is changed for each fish tank with the same water quality every day, a heating rod is used for keeping the temperature of water in the fish tank at 25 +/-1 ℃, the water is irradiated for 12 hours and is dark for 12 hours every day, the death condition of the grouper fries is recorded every day, dead fishes are timely fished out, and the water quality index is recorded every day;
3. analytical calculations
Analyzing the death condition of the grouper fries, calculating the average death rate according to the death number recorded each time, and calculating the half Lethal Concentration (LC) of toxic substances according to a regression equation50) And a 95% confidence interval, and then calculating the safe concentration.
The invention has the advantages that:
the device for carrying out toxicity experiments by using the grouper fries is strong in pertinence, accurate and efficient by fully considering characteristics and conditions of a marine water ecosystem and combining experimental requirements and material requirements. The device is more convenient and faster to carry out experiments, saves space, obtains more scientific and reliable toxicity experiment data, and is more suitable for deriving the water quality reference value of the aquatic organisms in the subsequent seawater.
Drawings
FIG. 1 is a schematic structural view of the present invention;
Detailed Description
With reference to the attached drawing 1, the device comprises a grouper fry breeding box (1), an experimental testing device (2), a storage box (3), a drainage tank (4), a fish feeding tank (5), a first fluorescent lamp (6), a first temperature controller (7), a first ventilating device (8), a first switch valve (9), a toxicant exposure fish tank (10), a second fluorescent lamp (11), a second temperature controller (12), a second ventilating device (13), a second switch valve (14), a drawable tank cover (15) and an outer discharge pipe orifice (16) which are arranged on the upper surface of an equipment box, toxic substance concentration values (17) are marked outside each toxicant exposure fish tank in the device, 12h illumination and 12h darkness are realized through the first fluorescent lamp (6) and the second fluorescent lamp (11), the experimental temperature is adjusted through the first temperature controller (7) and the second temperature controller (12), and oxygen is provided for the grouper fries through the first ventilating device (8) and the second ventilating device (13), the fish tank is cleaned and the waste water is discharged through the first switch valve (9) and the second switch valve (14), all parts of the experimental device can be detached, and the experimental device can be flexibly adjusted according to experimental contents and requirements.
An experimental method of a toxicity experimental device by using grouper fries comprises the following steps:
1. rockfish domestication
Collecting tap water, aerating for three days for dechlorination, adding seawater salt to prepare artificial seawater, selecting healthy grouper fries with body length of 5 +/-1 cm, feeding with the artificial seawater for 10 days, emptying intestinal tracts of the fish for one day after domestication is finished, and performing acute toxicity experiments;
2. acute toxicity test
(1) Preparing a toxic substance solution;
(2) preparing artificial seawater, wherein the mass ratio of water to seawater salt is 1: 0.145;
(3) setting four experimental groups and a blank control group, wherein the sizes of all the fish tanks are unified to 27L, the fish tanks are all provided with 2 parallel groups, the experimental groups I, II and III are experimental groups with different concentrations of toxic substances, the experimental group IV is a cosolvent experimental group, the blank group is an artificial seawater experimental group, and the four experimental groups and the blank group have the same conditions except that the water quality is different;
(4) putting 20 grouper fries selected in the step 1 into each fish tank to perform an acute toxicity experiment, wherein during the acute toxicity experiment, half of water is changed for each fish tank with the same water quality every day, a heating rod is used for keeping the temperature of water in the fish tank at 25 +/-1 ℃, the water is irradiated for 12 hours and is dark for 12 hours every day, the death condition of the grouper fries is recorded every day, dead fishes are timely fished out, and the water quality index is recorded every day;
3. analytical calculations
Comparing the death situations of the grouper seedlings in the experiment group I, the experiment group II, the experiment group III and the cosolvent experiment group and the blank control group, calculating the average death rate according to the death number recorded each time, converting the average death rate into a probability unit, calculating the concentration logarithm of the test solution, and solving the regression equation of the probability unit and the concentration logarithm of the test solution. Determining the median Lethal Concentration (LC) of toxic substances50) And 95% confidence intervals. And then empirical formula 96hLC50Calculate the safe concentration 0.1.
It should be understood by those skilled in the art that the toxicity test apparatus in China is different, and the above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention; all equivalent variations and modifications of the present invention are covered by the scope of the present invention.
Claims (4)
1. The device for carrying out toxicity experiments by using the grouper fries is characterized by comprising a grouper fry breeding and raising box (1), an experiment testing device (2), a storage box (3) and a drainage tank (4), wherein the grouper fry breeding and raising box (1) is provided with a feeding fish tank (5), a fluorescent lamp I (6), a temperature controller I (7) and a ventilation device I (8), the lower part of the feeding fish tank (5) controls water in a tank to be drained into the drainage tank (4) through a switch valve I (9), the experiment testing device (2) is provided with a poisoning fish tank (10), a fluorescent lamp II (11), a temperature controller II (12) and a ventilation device II (13), the lower part of the poisoning fish tank (10) controls water in the tank to be drained into the drainage tank (4) through a switch valve II (14), the equipment box (3) is convenient for classified storage, and the upper surface of the storage box (3) is a box cover (15) which can be pulled, the drainage channel (4) is provided with an outer discharge pipe orifice (16).
2. The apparatus for toxicity test using young grouper as claimed in claim 1, wherein each toxicant-contaminated fish tank is externally marked with a concentration value (17) of toxic substance.
3. The apparatus for toxicity test of Epinephelus coioides seedling as claimed in claim 1, wherein said fluorescent lamp is used for 12h of light and 12h of dark.
4. An experimental method for toxicity test of grouper fry as claimed in claims 1-3, wherein said experimental method comprises:
s1: rockfish domestication
Collecting tap water, aerating for three days for dechlorination, adding seawater salt to prepare artificial seawater, selecting healthy grouper fries with body length of 5 +/-1 cm, feeding with the artificial seawater for 10 days, emptying intestinal tracts of the fish for one day after domestication is finished, and performing acute toxicity experiments;
s2: acute toxicity test
(1) Preparing a toxic substance solution;
(2) preparing artificial seawater, wherein the mass ratio of water to seawater salt is 1: 0.145;
(3) setting four experimental groups and a blank control group, wherein the sizes of all the fish tanks are unified to 27L, the fish tanks are all provided with 2 parallel groups, the experimental groups I, II and III are experimental groups with different concentrations of toxic substances, the experimental group IV is a cosolvent experimental group, the blank group is an artificial seawater experimental group, and the four experimental groups and the blank group have the same conditions except that the water quality is different;
(4) putting 20 grouper fries selected in the step 1 into each fish tank to perform an acute toxicity experiment, wherein during the acute toxicity experiment, half of water is changed for each fish tank with the same water quality every day, a heating rod is used for keeping the temperature of water in the fish tank at 25 +/-1 ℃, the water is irradiated for 12 hours and is dark for 12 hours every day, the death condition of the grouper fries is recorded every day, dead fishes are timely fished out, and the water quality index is recorded every day;
s3: analytical calculations
Analyzing the death condition of the grouper fries, calculating the average death rate according to the death number recorded each time, and calculating the half Lethal Concentration (LC) of toxic substances according to a regression equation50) And a 95% confidence interval, and then calculating the safe concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011093275.6A CN112229972A (en) | 2020-10-14 | 2020-10-14 | Device for toxicity experiment by using grouper fries |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011093275.6A CN112229972A (en) | 2020-10-14 | 2020-10-14 | Device for toxicity experiment by using grouper fries |
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| Publication Number | Publication Date |
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| CN112229972A true CN112229972A (en) | 2021-01-15 |
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| CN202011093275.6A Pending CN112229972A (en) | 2020-10-14 | 2020-10-14 | Device for toxicity experiment by using grouper fries |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113156071A (en) * | 2021-04-14 | 2021-07-23 | 东莞理工学院 | Toxicological experiment device and method for evaluating toxicity of pesticide by using same |
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2020
- 2020-10-14 CN CN202011093275.6A patent/CN112229972A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113156071A (en) * | 2021-04-14 | 2021-07-23 | 东莞理工学院 | Toxicological experiment device and method for evaluating toxicity of pesticide by using same |
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| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210115 |