CN210596065U - Noninvasive bacterium collecting device for aquatic animals - Google Patents
Noninvasive bacterium collecting device for aquatic animals Download PDFInfo
- Publication number
- CN210596065U CN210596065U CN201921394211.2U CN201921394211U CN210596065U CN 210596065 U CN210596065 U CN 210596065U CN 201921394211 U CN201921394211 U CN 201921394211U CN 210596065 U CN210596065 U CN 210596065U
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- Prior art keywords
- sterile
- water inlet
- container
- filter membrane
- water
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- 241001465754 Metazoa Species 0.000 title claims abstract description 39
- 241000894006 Bacteria Species 0.000 title claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 92
- 239000012528 membrane Substances 0.000 claims abstract description 35
- 238000005192 partition Methods 0.000 claims abstract description 18
- 239000008223 sterile water Substances 0.000 claims abstract description 15
- 230000000384 rearing effect Effects 0.000 claims abstract description 13
- 244000144972 livestock Species 0.000 claims abstract description 10
- 239000011521 glass Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 4
- 238000007599 discharging Methods 0.000 description 4
- 238000001471 micro-filtration Methods 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The utility model discloses an aquatic livestock does not have bacteria collection system that creates, including temporarily raising container and circulating pump: the temporary culture container is of a box body structure and contains sterile water; the upper bottom of the box body is provided with a placing hole which is provided with a container cover; the upper part of the side wall is provided with a circular opening, the opening is connected with a pipeline matched with the opening, a hole partition plate is arranged in the pipeline, and a sterile microporous filter membrane is placed on one side of the hole partition plate facing the opening; the outlet of the pipeline is a water outlet; the lower part of the other side wall is provided with a water inlet, and the water outlet and the water inlet are positioned at two sides of the temporary rearing container; the circulating water inlet of the circulating pump is communicated with the water outlet through a hose, and the circulating water outlet is communicated with the water inlet through a hose. The aquatic animals discharge bacteria in or on the body surface of the aquatic animals into the sterile water, the sterile water sequentially flows through the sterile microporous filter membrane, the water outlet, the circulating water inlet, the circulating water outlet and the water inlet to form water circulation, and the bacteria in or on the body surface of the aquatic animals are left on the sterile microporous filter membrane.
Description
Technical Field
The utility model relates to an aquatic livestock does not have wound bacterium collection system belongs to auxiliary instrument technical field.
Background
In the traditional aquatic animal bacteria collection, tissues are usually cut from dead or dying animals or the body surfaces of the dead or dying animals, and then subsequent work such as bacteria isolation culture is carried out. The prior operation method comprises the steps of firstly fishing the aquatic animals from a farm, sending the aquatic animals to a microorganism laboratory, cutting part of tissues in or on the body surfaces of the aquatic animals by laboratory personnel, and carrying out subsequent bacteria separation culture identification. However, in practical situations, the farm is mostly built in a suburb and is far away from a microorganism laboratory, when aquatic animals are fished from the farm and sent to the laboratory, the animals generally die or are in an endangered state, and particularly in summer, the temperature is too high, and after the aquatic animals are dragged for a long time, animal tissues can be denatured and rotted, so that the detection result is influenced. And the quantity of samples to be detected is limited, the universality is lacked, and the inaccuracy of data for analyzing the bacterial composition in or on the body surface of the aquatic animals can be caused. Moreover, some aquatic animals with higher economic value have higher inspection cost and can also cause great waste. In order to improve the accuracy or universality of detection, reduce the cost and reduce the waste, a noninvasive aquatic animal bacteria collecting device capable of being operated on the site of a farm is needed.
Disclosure of Invention
The utility model aims to solve the technical problem that to exist not enough among the prior art, and provide an aquatic livestock does not have wound bacterium collection system, when gathering the internal or body surface bacterium of aquatic livestock, the cooperation is used this sampling device can be in the scene of breeding and is carried out the collection of no wound bacterium to a large amount of aquatic livestock live bodies in real time, after gathering the cluster, put back aquaculture pond with aquatic livestock again, very big improvement the accuracy and the universality that detect, reduce cost, it is extravagant to reduce, remedy current sampling method's not enough.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides an aquatic livestock does not have bacteria collection system that creates, is including temporarily raising container and circulating pump, and the two is connected its characterized in that through the hose:
the temporary culture container is a rectangular or square box body, and sterile water is contained in the box body; a rectangular or square placing hole is formed in the upper bottom of the box body, and a container cover is arranged in the placing hole;
the temporary culture container is characterized in that a circular opening is formed in the upper part of one side wall of the box body, the opening is connected with a pipeline matched with the opening, a circular porous partition plate is arranged in the pipeline, and a circular sterile microporous filter membrane is placed on one side, facing the opening, of the porous partition plate; the outlet of the pipeline is used as a water outlet;
the temporary rearing container is characterized in that a circular opening is formed in the lower part of the other side wall of the box body and serves as a water inlet, and the water outlet and the water inlet are located on two sides of the temporary rearing container;
the circulating pump is provided with a circulating water inlet and a circulating water outlet; the circulating water inlet is communicated with the water outlet through a hose, and the circulating water outlet is communicated with the water inlet through a hose;
the aquatic animals temporarily cultured in the temporary culture container discharge bacteria in or on the body surface into the sterile water, the sterile water sequentially flows through the sterile microporous filter membrane, the water outlet, the circulating water inlet, the circulating water outlet and the water inlet to form water circulation, and the bacteria in or on the body surface of the aquatic animals are left on the sterile microporous filter membrane.
In the technical scheme, the temporary culture container, the pipeline and the porous partition plate are integrated and are all made of organic glass; the container cover is made of organic glass.
In the technical scheme, the inner diameter of the pipeline is 55 mm; the diameter of the porous partition plate is 55 mm.
In the technical scheme, the diameter of the sterile microporous filter membrane is 50mm, and the sterile microporous filter membrane can just cover the porous partition plate; the sterile microfiltration membrane is preferably a 0.22 μm sterile microfiltration membrane.
The utility model discloses the application method of device does: when in use, the container cover is opened, the sterile microporous filter membrane is placed on the porous partition plate, and then sterile water (salinity is adjusted according to the growth environment of aquatic animals) is injected into the temporary rearing container. When collecting bacterial samples of aquatic animals, putting a certain amount of aquatic animals into the temporary rearing container through the placing holes, and covering the container cover; and (3) opening the circulating pump, discharging bacteria in the body or on the body surface of the aquatic livestock temporarily cultured in the temporarily-cultured container into the water body, allowing the water body in the temporarily-cultured container to enter the circulating water inlet through the sterile microporous filter membrane, allowing the bacteria in the water body to stay on the sterile microporous filter membrane under the filtration of the sterile microporous filter membrane, and returning the filtered water body to the temporarily-cultured container through the circulating water outlet. After working for 1-2 hours, opening the container cover, fishing out the temporarily cultured aquatic animals, putting the temporarily cultured aquatic animals back to the culture pond, discharging the water in the temporarily cultured containers, and lightly taking out the sterile microporous filter membrane by using tweezers, so that the method can be used for subsequent work such as bacteria culture identification and the like.
The utility model discloses the advantage of device lies in: when the aquatic animal noninvasive bacterium collection device collects the bacteria of the aquatic animals, the on-site real-time noninvasive living body collection mode is adopted, so that the detection accuracy and universality are greatly improved, the cost is reduced, the waste is reduced, and the defects of the existing sampling method are overcome.
Drawings
FIG. 1: the overall structure of the device of the utility model is shown schematically;
FIG. 2: the structure schematic diagram of the temporary rearing container in the device of the utility model;
FIG. 3: the utility model discloses a structure schematic diagram of a perforated clapboard in the device;
FIG. 4: the structure schematic diagram of the sterile microporous filter membrane in the device of the utility model;
wherein: 1. a temporary rearing container; 2. placing holes; 3. a container cover; 4. a pipeline; 5. a perforated baffle; 6. a sterile microporous filtration membrane; 7. a water outlet; 8. a water inlet; 9. a circulation pump; 10. a circulating water inlet; 11. a circulating water outlet; 12. a hose; 13. sterile water; the arrows represent the direction of water flow.
Detailed Description
The non-invasive bacteria collecting device for aquatic animals of the present invention will be described in detail with reference to the accompanying drawings.
An apparatus for non-invasive collection of bacteria from aquatic animals comprises a temporary container 1 and a circulation pump 9 connected by a hose 12, as shown in fig. 1-4:
the temporary culture container is a rectangular or square box body, and sterile water 13 is contained in the box body; a rectangular or square placing hole 2 is formed in the upper bottom of the box body, and a container cover 3 is arranged in the placing hole;
the temporary culture container is characterized in that a circular opening is formed in the upper part of one side wall of the box body, the opening is connected with a pipeline 4 matched with the opening, a circular porous partition plate 5 is arranged in the pipeline, and a circular sterile microporous filter membrane 6 is placed on one side, facing the opening, of the porous partition plate; the outlet of the pipeline is used as a water outlet 7;
the temporary rearing container is characterized in that a circular opening is formed in the lower part of the other side wall of the box body and serves as a water inlet 8, and a water outlet and a water inlet are formed in two sides of the temporary rearing container;
the circulating pump 9 is provided with a circulating water inlet 10 and a circulating water outlet 11; the circulating water inlet is communicated with the water outlet 7 through a hose 12, and the circulating water outlet 11 is communicated with the water inlet 8 through a hose;
the aquatic animals temporarily cultured in the temporary culture container discharge bacteria in or on the body surface into the sterile water, the sterile water sequentially flows through the sterile microporous filter membrane, the water outlet, the circulating water inlet, the circulating water outlet and the water inlet to form water circulation, and the bacteria in or on the body surface of the aquatic animals are left on the sterile microporous filter membrane.
In the utility model, the temporary culture container 1, the pipeline 4 and the perforated partition plate 5 are integrated and made of organic glass materials; the container cover 3 is made of organic glass.
In the utility model, the inner diameter of the pipeline 4 is 55 mm; the diameter of the porous partition plate 5 is 55 mm.
In the utility model, the aseptic microporous filter membrane 6 with the diameter of 50mm just can cover the perforated clapboard 5; the sterile microfiltration membrane is preferably a 0.22 μm sterile microfiltration membrane.
Use the utility model discloses during the device: when in use, the container cover is opened, the sterile microporous filter membrane is placed on the porous partition plate, and then sterile water (salinity is adjusted according to the growth environment of aquatic animals) is injected into the temporary rearing container. When collecting bacterial samples of aquatic animals, putting a certain amount of aquatic animals into the temporary rearing container through the placing holes, and covering the container cover; and (3) opening the circulating pump, discharging bacteria in the body or on the body surface of the aquatic livestock temporarily cultured in the temporarily-cultured container into the water body, allowing the water body in the temporarily-cultured container to enter the circulating water inlet through the sterile microporous filter membrane, allowing the bacteria in the water body to stay on the sterile microporous filter membrane under the filtration of the sterile microporous filter membrane, and returning the filtered water body to the temporarily-cultured container through the circulating water outlet. After working for 1-2 hours, opening the container cover, fishing out the temporarily cultured aquatic animals, putting the temporarily cultured aquatic animals back to the culture pond, discharging the water in the temporarily cultured containers, and lightly taking out the sterile microporous filter membrane by using tweezers, so that the method can be used for subsequent work such as bacteria culture identification and the like. The device can be used for collecting multiple batches of bacteria of aquatic animals in a farm, and compared with the traditional sampling method, the device greatly improves the accuracy and universality of detection due to the adoption of a field real-time noninvasive living body collection mode, reduces the cost and reduces the waste.
The above examples are merely illustrative of the technical concept and technical features of the present invention, and thus the scope of the present invention is not limited thereto. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (4)
1. The utility model provides an aquatic livestock does not have bacteria collection system that creates, includes foster container (1) and circulating pump (9), and the two is connected through hose (12), its characterized in that:
the temporary culture container (1) is a rectangular or square box body, and sterile water (13) is contained in the box body; a rectangular or square placing hole (2) is formed in the upper bottom of the box body, and a container cover (3) is arranged in the placing hole;
the temporary culture container is characterized in that a circular opening is formed in the upper portion of one side wall of the box body, the opening is connected with a pipeline (4) matched with the opening, a circular porous partition plate (5) is arranged in the pipeline, and a circular sterile microporous filter membrane (6) is placed on one side, facing the opening, of the porous partition plate; the outlet of the pipeline is used as a water outlet (7);
the temporary rearing container is characterized in that a circular opening is formed in the lower part of the other side wall of the box body and serves as a water inlet (8), and the water outlet and the water inlet are positioned on two sides of the temporary rearing container;
the circulating pump (9) is provided with a circulating water inlet (10) and a circulating water outlet (11); the circulating water inlet is communicated with the water outlet (7) through a hose (12), and the circulating water outlet (11) is communicated with the water inlet (8) through a hose;
the aquatic animals temporarily cultured in the temporary culture container discharge bacteria in or on the body surface into the sterile water, the sterile water sequentially flows through the sterile microporous filter membrane, the water outlet, the circulating water inlet, the circulating water outlet and the water inlet to form water circulation, and the bacteria in or on the body surface of the aquatic animals are left on the sterile microporous filter membrane.
2. The apparatus of claim 1, wherein: the temporary culture container (1), the pipeline (4) and the perforated partition plate (5) are integrated and are all made of organic glass; the container cover (3) is made of organic glass.
3. The apparatus of claim 1, wherein: the inner diameter of the pipeline (4) is 55 mm; the diameter of the porous partition plate (5) is 55 mm.
4. The apparatus of claim 1, wherein: the diameter of the sterile microporous filter membrane (6) is 50mm, and the sterile microporous filter membrane can just cover the porous partition plate (5); the sterile microporous filter membrane is a 0.22 mu m sterile microporous filter membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921394211.2U CN210596065U (en) | 2019-08-26 | 2019-08-26 | Noninvasive bacterium collecting device for aquatic animals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921394211.2U CN210596065U (en) | 2019-08-26 | 2019-08-26 | Noninvasive bacterium collecting device for aquatic animals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210596065U true CN210596065U (en) | 2020-05-22 |
Family
ID=70716272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921394211.2U Expired - Fee Related CN210596065U (en) | 2019-08-26 | 2019-08-26 | Noninvasive bacterium collecting device for aquatic animals |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210596065U (en) |
-
2019
- 2019-08-26 CN CN201921394211.2U patent/CN210596065U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200522 |