CN210596064U

CN210596064U - Water environment bacteria sampling device breeds

Info

Publication number: CN210596064U
Application number: CN201921383885.2U
Authority: CN
Inventors: 王淑娴; 叶海斌; 刁菁; 樊英; 李乐; 许拉
Original assignee: Shandong Marine Biology Institute
Current assignee: Shandong Marine Biology Institute
Priority date: 2019-08-24
Filing date: 2019-08-24
Publication date: 2020-05-22
Anticipated expiration: 2029-08-24

Abstract

The utility model discloses a breed water environment bacterium sampling device, including rechargeable miniature immersible pump and bacterium sample thief, the support fixed connection is passed through in the outside of the two together: the whole body of the bacteria sampler is of a cylindrical structure with one open end, and the open end is provided with a screw cap; the screw cap is provided with a circulating water inlet which is communicated with the outlet of the charging type micro submersible pump through a hose; and a circulating water outlet is formed in the closed end of the bacteria sampler and communicated with the aquaculture water environment through a hose. A circular perforated clapboard is arranged inside the bacteria sampler, and the plate surface of the perforated clapboard is parallel to the screw cap; one side of the porous clapboard facing the upper screw cap is covered with a sterile microporous filter membrane. Use the utility model discloses the bacterium of device collection compares traditional sampling bottle, and very labour saving and time saving to can guarantee that the bacterium kind of gathering is more, and quantity is bigger.

Description

Water environment bacteria sampling device breeds

Technical Field

The utility model relates to a water environment bacterium sampling device breeds belongs to auxiliary instrument technical field.

Background

The collection of bacteria in traditional aquaculture environments is typically done using collection bottles. The current operation method is characterized in that a plurality of collecting bottles are used for collecting water samples from the aquaculture water body at first, then the water samples are taken back to a suction filter funnel with a microporous filter membrane placed for suction filtration, and after the water sample suction filtration is finished, the filter membrane is taken out to carry out subsequent culture identification and other work of bacteria. However, in actual operation, in order to obtain as many bacteria as possible, a large amount of water samples need to be collected, which is time-consuming and labor-consuming, and the types and the number of the obtained bacteria are relatively limited, which finally results in inaccurate data for analyzing the bacterial composition in the aquaculture water environment. Therefore, a need exists for a device for collecting bacteria in a aquaculture environment that can provide a greater variety and number of species.

Disclosure of Invention

The utility model aims to solve the technical problem that to exist among the prior art not enough, and provide a breed water environment bacterium sampling device, when gathering the bacterium in the water environment, the cooperation uses the bacterium kind that this sampling device can very labour saving and time saving's realization was gathered more, and quantity is bigger, appears the error when avoiding analyzing the bacterium composition in the breed water environment to remedy the not enough of current sampling method.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a breed water environment bacterium sampling device, is including rechargeable miniature immersible pump and bacterium sample thief, and the outside of the two is passed through support fixed connection and is in the same place, its characterized in that:

rechargeable miniature immersible pump, be equipped with pump inlet and pump export, wherein: the inlet of the pump is communicated with the aquaculture water environment through a hose; the outlet of the pump is communicated with a bacteria sampler;

the whole bacteria sampler is of a cylindrical structure with one open end, and a screw cap is arranged at one open end; the screw cap is provided with a round small hole as a circulating water inlet which is communicated with the outlet of the pump of the rechargeable micro submersible pump through a hose; a circular small hole is also formed in the closed end of the bacteria sampler and serves as a circulating water outlet, and the circulating water outlet is communicated with the aquaculture water environment through a hose;

the bacteria sampler is internally provided with a circular perforated clapboard, and the surface of the perforated clapboard is parallel to the screw cap; one side of the porous clapboard facing the upper screw cap is covered with a sterile microporous filter membrane.

In the technical scheme, the support is tied with the rope, and when the rechargeable micro submersible pump and the bacteria sampler are placed in the aquaculture water environment, the bacteria sampler is transversely positioned in the aquaculture water environment.

In the technical scheme, the bacteria sampler is made of organic glass except for the sterile microporous filter membrane, and the whole material (comprising a screw cap and a porous partition plate) is organic glass.

Among the above-mentioned technical scheme, the bacterium sample thief, open-ended one end outside is equipped with the external screw thread, the screw cap is equipped with the internal thread, the two links together through rotatory mode.

In the technical scheme, the diameter of the bacteria sampler is 55 mm; the diameter of the porous partition plate is 55 mm; the diameter of the sterile microporous filter membrane is 50 mm.

In the technical scheme, the sterile microporous filter membrane is a 0.22-micron sterile microporous filter membrane.

The utility model discloses technical scheme's advantage lies in: the utility model discloses a breed water environment bacterium sampling device is when breeding the bacterium collection in the water environment, very labour saving and time saving to the bacterium variety that can realize gathering is more, and quantity is bigger, and the error appears when avoiding the bacterium composition in the analysis aquaculture water environment, compensaties the not enough of current sampling method.

Drawings

FIG. 1 is a schematic view of the whole structure of the bacteria sampling device for aquaculture environment of the present invention;

FIG. 2 is a schematic view of a screw cap and a circulating water inlet in the device of the present invention;

FIG. 3 is a schematic view of a screw cap, a circulating water inlet and a circulating water outlet in the device of the present invention;

FIG. 4 is a schematic view of a sterile microporous filter membrane and a porous partition plate in the device of the present invention;

FIG. 5 is a schematic view of a perforated baffle in the apparatus of the present invention;

wherein: 1. a rope; 2. a support; 3. a hose; 4. aquaculture water environment; 5. a charging type micro submersible pump 6 and a circulating water inlet; 7. a screw cap; 8. a sterile microporous filtration membrane; 9. a perforated baffle; 10. a bacteria sampler; 11. a circulating water outlet; arrows represent the direction of water flow.

Detailed Description

The following detailed description of the embodiments of the present invention is provided, but the present invention is not limited to the following descriptions:

a device for sampling bacteria in a culture water environment comprises a rechargeable micro submersible pump 5 and a bacteria sampler 10, wherein the exterior of the rechargeable micro submersible pump 5 and the exterior of the bacteria sampler 10 are fixedly connected together through a bracket 2, as shown in figures 1-5:

rechargeable miniature immersible pump, be equipped with pump inlet and pump export, wherein: the inlet of the pump is communicated with a culture water environment 4 through a hose 3; the outlet of the pump is communicated with a bacteria sampler;

the whole bacteria sampler 10 is of a cylindrical structure with one open end, and the open end is provided with a screw cap 7; a circular small hole is formed in the screw cap to serve as a circulating water inlet 6, and the circulating water inlet is communicated with an outlet of the charging type micro submersible pump through a hose 3; a circular small hole is also formed in the closed end of the bacteria sampler and serves as a circulating water outlet 11, and the circulating water outlet 11 is communicated with the aquaculture water environment 4 through a hose 3;

the bacteria sampler 10 is internally provided with a circular perforated clapboard 9, and the surface of the perforated clapboard is parallel to the screw cap; one side of the porous clapboard facing the upper screw cap is covered with a sterile microporous filter membrane 8;

the support is tied with a rope 1, and when the rechargeable micro submersible pump 5 and the bacteria sampler 10 are placed in the aquaculture water environment, the bacteria sampler is transversely positioned in the aquaculture water environment.

In the utility model, the bacteria sampler 10 is made of organic glass except the sterile microporous filter membrane.

The utility model discloses in, bacterium sample thief 10, the outside external screw thread that is equipped with of open-ended one end, screw cap 7 is equipped with the internal thread, the two links together through rotatory mode.

In the utility model, the diameter of the bacteria sampler 10 is 55 mm; the diameter of the porous partition plate 9 is 55 mm; the sterile microporous filter membrane 8 has the diameter of 50mm and can just cover the porous partition plate 9.

In the utility model, the aseptic microporous filter membrane 8 is a 0.22 μm aseptic microporous filter membrane.

The utility model discloses the application method of device does: when in use, the screw cap 7 is unscrewed, the sterile microporous filter membrane 8 is placed on the clapboard 9 with holes, and then the screw cap 7 is screwed tightly. Then, a circulating water inlet 6 on the screw cap 7 is communicated with an outlet of a pump of the rechargeable micro submersible pump 5 by using a hose, and the pump inlet is connected with the hose and then placed in the aquaculture water environment 4; the circulating water outlet 11 is connected with an upper hose and then placed in a device for containing or collecting water. When gathering the bacterium sample in the aquaculture water environment 4, with rope 1 system on support 2, the device slowly puts into the aquatic, opens rechargeable miniature immersible pump 5 under water, and the water in the aquaculture water environment 4 gets into circulating water entry 6 through rechargeable miniature immersible pump 5 this moment, and the bacterium in the water is stayed on the filter membrane under aseptic millipore filter membrane 8's filtration, and the water after the filtration is discharged through circulating water export 11. After working for 10min, the device is moved to other collection points to continue collecting bacteria. So gather several backs, close rechargeable miniature immersible pump 5, unscrew screw cap 7, take out aseptic millipore filtration membrane 8 gently with tweezers, can be used to work such as subsequent bacterial culture identification. Compared with the traditional sampling bottle, the bacteria collected by the device are very time-saving and labor-saving, and more types and larger quantities of the collected bacteria can be ensured.

The above examples are merely illustrative of the technical concept and technical features of the present invention, and thus the scope of the present invention is not limited thereto. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a breed water environment bacterium sampling device, includes rechargeable miniature immersible pump (5) and bacterium sample thief (10), and support (2) fixed connection is passed through to the outside of the two together, its characterized in that:

rechargeable miniature immersible pump, be equipped with pump inlet and pump export, wherein: the inlet of the pump is communicated with a culture water environment (4) through a hose (3); the outlet of the pump is communicated with a bacteria sampler;

the bacteria sampler (10) is integrally of a cylindrical structure with one open end, and a screw cap (7) is arranged at one open end; the screw cap is provided with a round small hole as a circulating water inlet (6), and the circulating water inlet is communicated with the outlet of the pump of the rechargeable micro submersible pump through a hose (3); a circular small hole is also formed in the closed end of the bacteria sampler and serves as a circulating water outlet (11), and the circulating water outlet is communicated with the aquaculture water environment through a hose;

the bacteria sampler (10) is internally provided with a circular perforated clapboard (9), and the surface of the perforated clapboard is parallel to the screw cap; the side of the porous clapboard facing the upper screw cap is covered with a sterile microporous filter membrane (8).

2. The aquatic environment bacteria sampling device of claim 1, wherein: the bracket is tied with a rope (1), and when the rechargeable micro submersible pump (5) and the bacteria sampler (10) are placed in the aquaculture water environment, the bacteria sampler is transversely positioned in the aquaculture water environment.

3. The aquatic environment bacteria sampling device of claim 1, wherein: the bacteria sampler (10) is made of organic glass except for the sterile microporous filter membrane.

4. The aquatic environment bacteria sampling device of claim 1, wherein: the sterile microporous filter membrane (8) is a 0.22 mu m sterile microporous filter membrane.

5. The aquatic environment bacteria sampling device of claim 1, wherein: the bacteria sampler (10) is characterized in that an external thread is arranged outside one end of the opening, an internal thread is arranged on the screw cap (7), and the two are connected together in a rotating mode.

6. The aquatic environment bacteria sampling device of claim 1, wherein: the diameter of the bacteria sampler (10) is 55 mm; the diameter of the porous partition plate (9) is 55 mm; the diameter of the sterile microporous filter membrane (8) is 50 mm.