CN102845367B - Automation running-water type cultivation device of daphnia magna - Google Patents
Automation running-water type cultivation device of daphnia magna Download PDFInfo
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- CN102845367B CN102845367B CN2012103635549A CN201210363554A CN102845367B CN 102845367 B CN102845367 B CN 102845367B CN 2012103635549 A CN2012103635549 A CN 2012103635549A CN 201210363554 A CN201210363554 A CN 201210363554A CN 102845367 B CN102845367 B CN 102845367B
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Abstract
The invention relates to an automation running-water type cultivation device of daphnia magna. The device comprises six components including a feed culturing room, a cultivation ground substance storage room, a female daphnia feeding room, a young daphnia feeding room, a return room and a power system, wherein water outlet tubes at bottoms of the feed culturing room and the cultivation ground substance storage room are connected with the female daphnia feeding room through the power system; a water outlet tube at the bottom of the female daphnia feeding room is connected with the top of the young daphnia feeding room; a water outlet tube on the top of the young daphnia feeding room is connected with the top of the return room; and a water outlet tube at the bottom of the return room is connected with the cultivation ground substance storage room.
Description
Technical field
The invention belongs to plankton cultivation field, be specifically related to a kind of Daphnia magna automation water-flowing type aquaculture device.
Background technology
Daphnia magna (Daphnia magna Straus) belongs to Arthropoda, Crustachia, cladocera, is common crustacean plankton, is indispensable constituent in the fresh water food chain.Because its life cycle is short, fast, high, the easy cultivation of reproduction amount of growth, water environment coerced to the characteristics such as responsive make it become the widely used model organism in domestic and international laboratory, there is important effect aspect the test of the acute toxicity and chronic toxicity of agricultural chemicals, chemicals and environmental hormone effects.
A plurality of international organizations such as current European OECD (OECD), European Union, the U.S., Japan, Korea S and China and country have all formed systematized Daphnia magna toxicity testing standard method.The method is all made and being explicitly called for length of time of Daphnia magna, culture medium, condition of culture etc. test.Yet the Daphnia magna in the most of laboratories of China cultivation at present lacks effective equipment or device, only can rely on mechanical manual operation, and not only efficiency is low in this manual operation, workload is large, and be difficult to obtain the test young Magna in the required specific length of time.This brings certain difficulty and larger error for usining Daphnia magna as the multiple testing of biological subject.
Summary of the invention
The objective of the invention is to overcome the defect of existing artificial Daphnia magna cultural technique, a kind of Daphnia magna automation water-flowing type aquaculture device is provided.
This Daphnia magna automatic breeding device comprises feed culturing room, cultivation matrix apotheca, female Magna receptacle, young Magna receptacle, return passage and six parts of dynamical system; The bottom of described feed culturing room and cultivation matrix apotheca sets out water pipe, flowing water Valve and aeration valve, and feed culturing room one side is installed growth lamp, and feed culturing room is connected with female Magna receptacle by peristaltic pump respectively with the outlet pipe of cultivation matrix apotheca; Female Magna receptacle and young Magna receptacle are the column container of bottom taper, there are filter screen and flowing water Valve in the cylinder of female Magna receptacle and the junction of cone, flowing water Valve is positioned on filter screen, children Magna receptacle bottom sets out water pipe and flowing water Valve, the bottom outlet pipe of female Magna receptacle is connected with young Magna receptacle, and the pipe end that stretches into an end of young Magna receptacle connects a glass vertical tube, described glass vertical tube is connected with atmosphere, children Magna receptacle is connected with return passage by a water outlet straight tube that approaches top, water outlet straight tube mouth parcel filter screen; Return passage arranges a right angle overflow pipe near a mouthful place, top, and its height is a little less than the straight tube be connected with young Magna receptacle, and return passage bottom outlet pipe is connected with cultivation matrix apotheca; Described dynamical system, comprise two peristaltic pumps and siphonic system, two peristaltic pumps have respectively separately independently Timer Controlling, peristaltic pump and siphonic system are arranged between feed culturing room and female Magna receptacle, and another peristaltic pump and siphonic system are arranged between cultivation matrix apotheca and female Magna receptacle.
Described feed culturing room is for cultivating the culturing room of green alga;
Described female Magna receptacle and young Magna receptacle are column container transparent, the inertia material;
The pipe end that described female Magna receptacle links with young Magna receptacle (stretching into an end of young Magna receptacle) connects a glass vertical tube, and this vertical tube top is connected with atmosphere.
The column container of transparent, the inertia material that wherein, described return passage is the bottom taper.
Wherein, described cultivation matrix apotheca top and return passage are with high.
Operation logic of the present invention is as follows:
In the A of feed culturing room disposable inoculation green alga and keep suitable condition (22～25 ℃ of temperature, illumination 3000lux, bottom aeration) to cultivate 5～7 days after, can form the Daphnia magna algae liquid of feeding.Pour respectively appropriate medium in Daphnia magna cultivation matrix apotheca B, female Magna receptacle C, young Magna receptacle D and return passage E, until liquid level reaches alpha levels.
Adopt timer 2,4 to control respectively peristaltic pump 1,3, the peristaltic pump flow can be regulated as required voluntarily.
Access healthy, great-hearted Daphnia magna (density is 100～200 every liter) in female Magna receptacle C, start whole device.Now peristaltic pump 1 regularly enters female Magna receptacle C by a small amount of algae liquid pump, and peristaltic pump 3 regularly pumps into female Magna receptacle C by the fresh cultivation medium of part.And, according to siphon principle, the reduction of B liquid level must cause the medium back flow in return passage E, the D medium flows in E, and the C medium flows in D, so just forms the dynamic circulation system of B-C-D-E-B.In the water circulation process, the young Magna that female Magna produces just can constantly enter in young Magna receptacle D with current by the filter screen of female Magna receptacle C bottom from C, realizes that young Magna regularly collects; Algae liquid can be brought in C, D with current, realizes feeding at regular time and quantity to female Magna and young Magna; Fresh medium also can constantly enter in C, D with current, realizes dynamically updating of culture medium.In the flow process of current from D to E, the existence of delivery port filter screen prevents that young Magna from flowing out on the one hand, filters out on the other hand particle, guarantees the clean of recirculation water.According to operator's demand, select the suitable time can from D, collect the young Magna in the suitable length of time.
The present invention has following beneficial effect:
1, can realize the dynamic cultivation green alga;
2, the Daphnia magna of feeding at regular time and quantity to automation, greatly reduce manpower, and scale of feeding more precisely, feeding time is more reasonable;
3, realize the Daphnia magna cultivating system MEDIA FLOW ability of swimming circulation, upgrade, avoid the Daphnia magna anoxic and suppressed or dead, reduce numerous and diverse work of artificial changing water;
4, optionally collect the young Magna in the different length of times, for the Daphnia magna toxotest provides applicable tested Magna, save a large amount of manpowers;
5, round Realization automation of the present invention, cost is low, is easy to realize commercially producing and promoting.
The accompanying drawing explanation:
Fig. 1: the structural representation of Daphnia magna automatic breeding device of the present invention.
Embodiment:
Below in conjunction with accompanying drawing, the present invention is described in further details.
This specific embodiment is only explanation of the invention; it is not limitation of the present invention; those skilled in the art can make to the present embodiment the modification that there is no creative contribution as required after reading this specification, but as long as all are subject to the protection of Patent Law in claim scope of the present invention.
Embodiment 1: Daphnia magna automatic breeding device as shown in Figure 1, and wherein each symbol or letter representation are as follows: A-feed culturing room; B-cultivation matrix apotheca; The female Magna receptacle of C-; D-children Magna receptacle; The E-return passage; 1,3 be peristaltic pump; 2,4 be timer; The 5-valve; The 6-35 mesh filter screen; The 7-80 mesh filter screen; The 8-vertical tube; α, β-waterline.
This title and size of installing each main member is as follows:
The A of feed culturing room: diameter 20cm, height 100cm, capacity is 25L
Cultivation matrix apotheca B: diameter 20cm, height 100cm, capacity is 25L
Female Magna receptacle C: diameter 20cm, height 40cm, capacity is 10L
Children Magna receptacle D: diameter 20cm, height 40cm, capacity is 10L
Return passage E: diameter 10cm, height 40cm, capacity is 3.0L
Peristaltic pump 1 and 2:5 (ml/min)
Valve 5: copper
Filter screen 6: stainless steel, mesh 35 orders;
Filter screen 7: nylon material, mesh 80 orders;
Vertical tube 8: diameter 1cm, high 30cm;
Right angle overflow pipe 9: diameter 0.5cm;
All band growth lamp 10: power 60w.
The A of feed culturing room is connected with female Magna receptacle C by peristaltic pump respectively with cultivation matrix apotheca B; Female Magna receptacle C is connected with young Magna receptacle D by the bottom outlet pipe, and tube connector gos deep into a vertical tube 8 of the vertical connection of an end of young Magna receptacle, and the vertical tube top connects air; Children Magna receptacle is connected with return passage E by a water outlet straight tube, 3 layer of 80 purpose nylon wire of water outlet straight tube mouth place parcel; Return passage bottom outlet pipe is connected with cultivation matrix apotheca B, approaches place, return passage top, than the connected lower slightly 1cm At The Height of straight tube (being β-waterline place) of young Magna receptacle, right angle overflow pipe 9 is set.
The method of operating of Daphnia magna automatic breeding device of the present invention comprises the steps:
1) algae liquid feed is cultivated
1. the A of closure feed culturing room flowing water Valve, the medium to adding 20L in the A of feed culturing room, then add the appropriate chlorella vulgaris (or other algae) in increased logarithmic phase, makes its initial frustule concentration reach 10
4～10
5Individual/mL.
2. open the A of feed culturing room bottom aeration valve continuous micro aeration, open growth lamp.
3. after 5 days, frustule concentration rises to 10
5～10
6Individual/mL
4. add medium 2 times in every circumferential feed A of culturing room, each 3000mL.
2) female Magna inoculation and raising
1. to the medium that adds respectively aeration 24h in female Magna receptacle C, young Magna receptacle D, until liquid level all reaches alpha levels; To cultivating the medium that adds aeration 24h in matrix apotheca B and return passage E, until liquid level all reaches the β level.Because liquid level (alpha levels) in C, D exceeds B, E liquid level (β level) 1cm, during therefore under siphonage, in C, D, liquid flows into E successively, once in E, liquid level is higher than the β level, liquid overflow by right angle overflow pipe 9.
2. open cultivation matrix apotheca B bottom aeration valve and continue aeration.
3. add 200～250 health, great-hearted Daphnia magna in female Magna receptacle C.
4. timer 2 and 4 is set as respectively per hour starting 5min, opens peristaltic pump, under time set, peristaltic pump per hour moves 5 minutes, carries respectively medium and the algae liquid feed of oxygen enrichment to female Magna receptacle and young Magna receptacle.
When 3. peristaltic pump starts, in B, liquid level is lower than the β level, and under siphonage, in E, liquid backflow enters in B until B, E liquid level maintain an equal level.
4. after 8 days, Daphnia magna produces the little Magna of the first-born, and when peristaltic pump starts, under current drive, young Magna will enter young Magna receptacle by the filter screen of female Magna receptacle bottom, and algae liquid also is admitted to young Magna receptacle simultaneously.The existence of vertical tube 8 has stoped young Magna oppositely to swim in female Magna receptacle C.
3) young Magna collects
1. new born young Magna per hour collects once to young Magna receptacle, according to research, need to get the young Magna of Magna n in age hour, can be after n hour, and the valve of opening young Magna receptacle side carries out young Magna collection.
A kind of Daphnia magna automatic breeding device of the present invention is described by concrete example, those skilled in the art can use for reference content of the present invention, the links such as appropriate change raw material, process conditions realize corresponding other purpose, its relevant change does not all break away from content of the present invention, within all similar replacements and change will become apparent to those skilled in the art that and all be deemed to be included in scope of the present invention.
Claims (1)
1. a Daphnia magna automation water-flowing type aquaculture device is characterized in that: described cultivator comprises feed culturing room, cultivation matrix apotheca, female Magna receptacle, young Magna receptacle, return passage and six parts of dynamical system; The bottom of described feed culturing room and cultivation matrix apotheca sets out water pipe, flowing water Valve and aeration valve, and feed culturing room one side is installed growth lamp, and feed culturing room is connected with female Magna receptacle by peristaltic pump respectively with the outlet pipe of cultivation matrix apotheca; Female Magna receptacle and young Magna receptacle are the column container of bottom taper, there are filter screen and flowing water Valve in the cylinder of female Magna receptacle and the junction of cone, flowing water Valve is positioned on filter screen, children Magna receptacle bottom sets out water pipe and flowing water Valve, the bottom outlet pipe of female Magna receptacle is connected with young Magna receptacle, children Magna receptacle is connected with return passage by a water outlet straight tube that approaches top, water outlet straight tube mouth parcel filter screen; Return passage arranges a right angle overflow pipe near a mouthful place, top, and its height is a little less than the straight tube be connected with young Magna receptacle, and return passage bottom outlet pipe is connected with cultivation matrix apotheca; Described dynamical system, comprise two peristaltic pumps and siphonic system, two peristaltic pumps have respectively separately independently Timer Controlling, peristaltic pump and siphonic system are arranged between feed culturing room and female Magna receptacle, and another peristaltic pump and siphonic system are arranged between cultivation matrix apotheca and female Magna receptacle; Described feed culturing room is for cultivating the culturing room of green alga; Described female Magna receptacle and young Magna receptacle are column container transparent, the inertia material; That be connected an and pipe end end that stretch into young Magna receptacle of described female Magna receptacle and young Magna receptacle is connected a glass vertical tube, and described glass vertical tube is connected with atmosphere; The column container of transparent, the inertia material that described return passage is the bottom taper; Described cultivation matrix apotheca top and return passage are with high.
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| CN2012103635549A CN102845367B (en) | 2012-09-26 | 2012-09-26 | Automation running-water type cultivation device of daphnia magna |
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| CN2012103635549A CN102845367B (en) | 2012-09-26 | 2012-09-26 | Automation running-water type cultivation device of daphnia magna |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563859A (en) * | 2013-11-18 | 2014-02-12 | 华中农业大学 | Device and method for replacing nutrient solution for indoor cultivation of daphnia magna |
| CN103598150B (en) * | 2013-11-30 | 2015-12-02 | 哈尔滨工业大学 | Daphnia magna laboratory fast culture device and cultural method |
| CN104026081A (en) * | 2014-06-11 | 2014-09-10 | 温州中普知识产权有限公司 | Raising device for crustacean plankton |
| CN106442959B (en) * | 2016-10-10 | 2018-09-07 | 环境保护部南京环境科学研究所 | Daphnia magna continuous-flow type toxicity test device |
| CN107318748A (en) * | 2017-05-14 | 2017-11-07 | 上海海洋大学 | A kind of Biological control device for aquarium |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653106A (en) * | 2009-09-22 | 2010-02-24 | 中国水产科学研究院渔业机械仪器研究所 | Closed circulating rotifer continuous culturing system |
| CN202773798U (en) * | 2012-09-26 | 2013-03-13 | 环境保护部南京环境科学研究所 | Daphnia magna automation running form breeding device |
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|---|---|---|---|---|
| JP2589248B2 (en) * | 1992-05-06 | 1997-03-12 | 社団法人マリノフォーラム二十一 | Continuous zooplankton culture method and its equipment. |
| JP3786736B2 (en) * | 1995-12-27 | 2006-06-14 | 社団法人マリノフォーラム二十一 | Method for continuous culture of zooplankton |
| CN1611111A (en) * | 2000-07-06 | 2005-05-04 | 长瀬产业株式会社 | Culturing articles for non-concentrated bait and its production method |
| CN202256019U (en) * | 2011-09-15 | 2012-05-30 | 中国水产科学研究院长江水产研究所 | Fully automatic plankton sample concentration treatment device |
| CN102773798B (en) * | 2012-07-21 | 2014-09-03 | 北京工业大学 | Test piece clamping device for force feedback adaptive grinding and polishing machine |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653106A (en) * | 2009-09-22 | 2010-02-24 | 中国水产科学研究院渔业机械仪器研究所 | Closed circulating rotifer continuous culturing system |
| CN202773798U (en) * | 2012-09-26 | 2013-03-13 | 环境保护部南京环境科学研究所 | Daphnia magna automation running form breeding device |
Non-Patent Citations (2)
| Title |
|---|
| JP特开平6-165625A 1994.06.14 |
| JP特开平9-172902A 1997.07.08 |
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