CN104026081A - Raising device for crustacean plankton - Google Patents
Raising device for crustacean plankton Download PDFInfo
- Publication number
- CN104026081A CN104026081A CN201410270972.2A CN201410270972A CN104026081A CN 104026081 A CN104026081 A CN 104026081A CN 201410270972 A CN201410270972 A CN 201410270972A CN 104026081 A CN104026081 A CN 104026081A
- Authority
- CN
- China
- Prior art keywords
- crustacean
- receptacle
- young
- female
- plankton
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a raising device for crustacean plankton. The raising device comprises a feed culture room, a raising substrate storage chamber, a female crustacean plankton raising chamber, a young crustacean plankton raising chamber, a return chamber and a power system. The female crustacean plankton raising chamber and the young crustacean plankton raising chamber are transparent glass cylindrical containers. An aspen resin layer is connected to the inner wall of the bottom conical part of the young crustacean plankton raising chamber. The pipeline end of one end, connected with and extending into the young crustacean plankton raising chamber, of the female crustacean plankton raising chamber is connected with a vertical glass tube connected with the atmosphere. The return chamber is a transparent glass cylindrical container with a conical bottom. The top of the raising substrate storage chamber is as high as the return chamber. The raising device has the advantages the crustacean plankton raised by the device is healthy and free of germs, mechanical automatic running water type raising is achieved, manpower is saved, and cost is lowered greatly.
Description
Technical field
The present invention relates to the raising device of crustacean plankton.
Background technology
Crustacean plankton of the prior art, because of its growth cycle short, growth is fast, high, the easy cultivation of reproduction amount, water environment is coerced to the feature such as responsive become the widely used model organism in domestic and international laboratory, aspect the acute toxicity and chronic toxicity of agricultural chemicals, chemicals and the test of environmental hormone effects, has important function.
A plurality of international organizations such as current Europe, the U.S., Japan, Korea S and China and country have all formed systematization crustacean plankton toxicity testing standard method.The method is all made and being explicitly called for the length of time of the crustacean plankton of test use, culture medium, condition of culture etc.Yet the crustacean plankton in the most of laboratories of China lacks effective equipment or device at present, only can rely on manual operation, not only efficiency is low, workload is large, and being difficult to obtain the test young crustacean plankton in the required specific length of time, this brings certain difficulty and larger error to testing.
Summary of the invention
In view of this, technical problem to be solved by this invention is: provide a kind of crustacean plankton of raising out more healthy, without germ, and the raising device of the crustacean plankton raised of mechanical automation continuous-flow type.
In order to achieve the above object, the present invention adopts following technical scheme to realize:
The invention provides the raising device of a kind of crustacean plankton, wherein, described cultivator comprises feed culturing room, cultivation matrix apotheca, female crustacean receptacle, young crustacean receptacle, return passage and six parts of dynamical system; The bottom of described feed culturing room and cultivation matrix apotheca sets out water pipe, flowing water Valve and aeration valve, feed culturing room one side is installed growth lamp, and feed culturing room is connected with female crustacean receptacle by peristaltic pump respectively with the outlet pipe of cultivation matrix apotheca; Female crustacean receptacle and young crustacean receptacle are the column container of bottom taper, there are filter screen and flowing water Valve in the cylinder of female crustacean receptacle and the junction of cone, flowing water Valve is positioned on filter screen, children crustacean receptacle bottom sets out water pipe and flowing water Valve, the bottom outlet pipe of female crustacean receptacle is connected with young crustacean receptacle, children crustacean receptacle is connected with return passage by approaching a water outlet straight tube at top, water outlet straight tube mouth parcel filter screen; Return passage arranges a right angle overflow pipe near a mouthful place, top, and its height is a little less than the straight tube being connected with young crustacean receptacle, and return passage bottom outlet pipe is connected with cultivation matrix apotheca; Described dynamical system, comprise two peristaltic pumps and siphonic system, two peristaltic pumps have respectively separately independently Timer Controlling, peristaltic pump and siphonic system are arranged between feed culturing room and female crustacean receptacle, and another peristaltic pump and siphonic system are arranged between cultivation matrix apotheca and female crustacean receptacle; Described feed culturing room is for cultivating the culturing room of green alga; Described female crustacean receptacle and young crustacean receptacle are the column container of transparent glass material; On the inwall of the bottom tapering part of described young crustacean receptacle, be connected with willow resin bed, described female crustacean receptacle is connected a glass vertical tube with young crustacean receptacle pipe end that be connected and that stretch into one end of young crustacean receptacle, and described glass vertical tube is connected with atmosphere; Described return passage is the column container of transparent, the glass material of bottom taper; Described cultivation matrix apotheca top and return passage are with high.
As preferably, on described female crustacean plankton receptacle inwall, be connected with the Moschus piece with the ventilative shell of glass, on the ventilative shell of described glass, have a plurality of small ventilating holes, the ventilative shell of described glass is pasted on described female crustacean plankton receptacle inwall by glue.
Operation logic of the present invention is as follows:
Disposable inoculation green alga keep suitable condition (temperature 22-25 ℃, illumination 3000lux, bottom aeration) to cultivate after 5-7 days in the A of feed culturing room, can form the crustacean plankton algae liquid of feeding.In crustacean plankton cultivation matrix apotheca B, female crustacean receptacle C, young crustacean receptacle D and return passage E, pour respectively appropriate medium into, until liquid level reaches alpha levels.
Adopt timer 2,4 to control respectively peristaltic pump 1,3, peristaltic pump flow can regulate as required voluntarily.
In female crustacean receptacle C, access healthy, great-hearted crustacean plankton (density is every liter of 100-200), start whole device, now peristaltic pump 1 regularly enters female crustacean receptacle C by a small amount of algae liquid pump, and peristaltic pump 3 regularly pumps into female crustacean receptacle C by the fresh cultivation medium of part.And according to siphon principle, the reduction of B liquid level must cause the medium back flow in return passage E, D medium flows in E, and C medium flows in D, so just forms the dynamic circulation system of B-C-D-E-B.In water circulation process, the young crustacean plankton that female crustacean plankton is produced just can constantly enter in young crustacean receptacle D with current by the filter screen of female crustacean receptacle C bottom from C, realizes young crustacean plankton and regularly collects; Algae liquid can be brought in C, D with current, realizes feeding at regular time and quantity to female crustacean plankton and young crustacean plankton; Fresh medium also can constantly enter in C, D with current, realizes dynamically updating of culture medium.In the flow process of current from D to E; the existence of delivery port filter screen prevents young crustacean plankton outflow on the one hand, filters out on the other hand particle, guarantees the clean of recirculation water; according to operator's demand, select the suitable time can from D, collect the young crustacean plankton in the suitable length of time.
From above scheme, beneficial effect of the present invention is:
1, can realize dynamic cultivation green alga;
2, the crustacean plankton of feeding at regular time and quantity to automation, greatly reduces manpower, and scale of feeding more accurately, feeding time is more reasonable.
3, realize crustacean plankton cultivating system MEDIA FLOW ability of swimming circulation, upgrade, avoid crustacean plankton anoxic and suppressed or dead, reduce numerous and diverse work of artificial changing water.
4, optionally collect the young crustacean plankton in the different length of times, for crustacean plankton toxotest provides applicable tested crustacean plankton, save a large amount of manpowers.
5, round Realization automation of the present invention, cost is low, is easy to realize commercially produce and promote.
6, the present invention is by having connected willow resin bed on the lower end bullet inwall at young crustacean receptacle, through experiment, record, can make young crustacean plankton more easily conform, there is sedative action, children crustacean plankton enters after this children crustacean receptacle, still can as near growth parent, loosen, easy, through many experiments, record, young crustacean plankton during this children crustacean is raised indoor culture is more healthy, not portable germ, basic in the anosis state of health, can greatly improve the authenticity of experimental data, Stability and veracity, and in the situation that not adding this willow resin bed the more portable germ of young crustacean plankton, and the young crustacean plankton of 5% left and right is in inferior health or sick state, the accuracy that is unfavorable for very much experimental data, error is very large.
Accompanying drawing explanation
Fig. 1 is the structural representation of the raising device of crustacean plankton of the present invention.
Embodiment
In order to make those skilled in the art can further understand feature of the present invention and technology contents, refer to following about detailed description of the present invention and accompanying drawing.
Embodiment 1:
Refer to shown in Fig. 1, the invention provides the raising device of a kind of crustacean plankton, wherein, each symbol and letter representation are as follows:
A-feed culturing room; B-cultivation matrix apotheca; The female crustacean receptacle of C-; D-children crustacean receptacle; E-return passage; 1,3 be peristaltic pump; 2,4 be timer; 5-valve; 6-35 mesh filter screen; 7-80 mesh filter screen; 8-vertical tube; α, β-waterline.
This title and size of installing each main member is as follows:
The A of feed culturing room: diameter 20cm, height 100cm, capacity is 25L
Cultivation matrix apotheca B: diameter 20cm, height 100cm, capacity is 25L
Female crustacean receptacle C: diameter 20cm, height 40cm, capacity is 10L
Children crustacean receptacle D: diameter 20cm, height 40cm, capacity is 10L
Return passage E: diameter 10cm, height 40cm, capacity is 3.0L
Peristaltic pump 1 and 2:5 (ml/min)
Timer 3 and 4: startup 5min per hour
Valve 5: copper
Filter screen 6: stainless steel, mesh 35 orders
Filter screen 7: nylon material, mesh 80 orders
Vertical tube 8: diameter 1cm, high 30cm
Right angle overflow pipe 9: diameter 0.5cm
All band growth lamp 10: power 60w
The A of feed culturing room is connected with female crustacean receptacle C by peristaltic pump respectively with cultivation matrix apotheca B; Female crustacean receptacle C is connected with young crustacean receptacle D by bottom outlet pipe, and tube connector gos deep into a vertical tube 8 of the vertical connection in one end of young crustacean receptacle, and vertical tube top connects air; Children crustacean receptacle is connected with return passage E by a water outlet straight tube, 3 layer of 80 object nylon wire of water outlet straight tube mouth place parcel; Return passage bottom outlet pipe is connected with cultivation matrix apotheca B, approaches place, return passage top, than the connected lower slightly 1cm At The Height of straight tube (being β-waterline place) of young crustacean receptacle, right angle overflow pipe 9 is set.
The method of operating of the raising device of crustacean plankton of the present invention comprises the steps:
1) algae liquid feed is cultivated
1. the A of closure feed culturing room flowing water Valve, the medium to adding 20L in the A of feed culturing room, then adds the appropriate chlorella vulgaris (or other algae) in increased logarithmic phase, makes its initial frustule concentration reach 10
4-10
5individual/ml.
2. open the A of feed culturing room bottom aeration valve continuous micro aeration, open growth lamp.
3. after 5 days, frustule concentration rises to 10
5-10
6individual/ml
4. in every circumferential feed A of culturing room, add medium 2 times, each 3000ml.
2) female crustacean plankton inoculation and raising
1. to the medium that adds respectively aeration 24h in female crustacean receptacle C, young crustacean receptacle D, until liquid level all reaches alpha levels; To cultivating the medium that adds aeration 24h in matrix apotheca B and return passage E, until liquid level all reaches β level.Because the liquid level (alpha levels) of C, D exceeds B, E liquid level (β level) 1cm, therefore under siphonage, in C, D, liquid flows in E successively, once liquid level is higher than β level in E, liquid overflow by right angle overflow pipe 9.
2. open cultivation matrix apotheca B bottom aeration valve and continue aeration.
3. in female crustacean receptacle C, add only healthy, great-hearted crustacean plankton of 200-250.
4. timer 2 and 4 is set as respectively to startup 5min per hour, opens peristaltic pump, under time set, peristaltic pump operation per hour 5 minutes, carries respectively medium and the algae liquid feed of oxygen enrichment to female crustacean receptacle and young crustacean receptacle.
When 5. peristaltic pump starts, in B, liquid level is lower than β level, and under siphonage, in E, liquid backflow enters in B until B, E liquid level maintain an equal level.
6. after 8 days, crustacean plankton produces the little crustacean plankton of the first-born, when peristaltic pump starts, under current drive, children crustacean plankton enters young crustacean plankton receptacle by the filter screen of female crustacean plankton receptacle bottom, and algae liquid is also admitted to young crustacean plankton receptacle simultaneously.The existence of vertical tube 8 has stoped young crustacean plankton oppositely to be swum in female crustacean plankton receptacle C.
3) young crustacean plankton is collected
1. new born young crustacean plankton is per hour collects once to young crustacean receptacle, according to research, need to get the young crustacean plankton young crustacean plankton of n in age hour, can be after n hour, the valve of opening young crustacean receptacle side carries out young crustacean plankton collection.
Female crustacean receptacle described in the present invention and young crustacean receptacle are the column container of transparent glass material, on the inwall of the bottom tapering part of described young crustacean receptacle, be connected with willow resin bed, its connected mode is very simple, can will after resin heating and melting, be coated on glass inwall, when solid-state, cement on glass inwall, or resin is made and the pyramidal structure housing of the bottom tapering part adaptation of the young crustacean receptacle (position of the valve on corresponding young crustacean receptacle or delivery port also corresponding port that is provided with on it certainly by taper die, and can not seal up), be enclosed within on the inwall of bottom tapering part that inside is connected to described young crustacean receptacle.
Meanwhile, on described female crustacean plankton receptacle C inwall, be connected with the Moschus piece with the ventilative shell of glass, on the ventilative shell of described glass, there are a plurality of small ventilating holes, and be pasted on described female crustacean plankton receptacle C inwall by glue.Through experiment, record, connected after this Moschus piece, female crustacean plankton reproduction rate in female crustacean plankton receptacle C obviously improves, female crustacean plankton receptacle C that in one month, the young crustacean plankton of breeding does not connect Moschus piece compared with another has increased by 10%, and female crustacean plankton number in age, quantity and other parameters before both are all identical.Described Moschus piece is preferably and is connected to female crustacean plankton receptacle and is positioned on the inwall of waterline α upper end.
But the foregoing is only better possible embodiments of the present invention, not in order to limit to the scope of the claims of the present invention, thus the equivalent structure that all utilizations specification of the present invention and accompanying drawing content are done variation, all in like manner within the scope of the present invention.
Claims (2)
1. a raising device for crustacean plankton, is characterized in that, described cultivator comprises feed culturing room, cultivation matrix apotheca, female crustacean receptacle, young crustacean receptacle, return passage and six parts of dynamical system; The bottom of described feed culturing room and cultivation matrix apotheca sets out water pipe, flowing water Valve and aeration valve, feed culturing room one side is installed growth lamp, and feed culturing room is connected with female crustacean receptacle by peristaltic pump respectively with the outlet pipe of cultivation matrix apotheca; Female crustacean receptacle and young crustacean receptacle are the column container of bottom taper, there are filter screen and flowing water Valve in the cylinder of female crustacean receptacle and the junction of cone, flowing water Valve is positioned on filter screen, children crustacean receptacle bottom sets out water pipe and flowing water Valve, the bottom outlet pipe of female crustacean receptacle is connected with young crustacean receptacle, children crustacean receptacle is connected with return passage by approaching a water outlet straight tube at top, water outlet straight tube mouth parcel filter screen; Return passage arranges a right angle overflow pipe near a mouthful place, top, and its height is a little less than the straight tube being connected with young crustacean receptacle, and return passage bottom outlet pipe is connected with cultivation matrix apotheca; Described dynamical system, comprise two peristaltic pumps and siphonic system, two peristaltic pumps have respectively separately independently Timer Controlling, peristaltic pump and siphonic system are arranged between feed culturing room and female crustacean receptacle, and another peristaltic pump and siphonic system are arranged between cultivation matrix apotheca and female crustacean receptacle; Described feed culturing room is for cultivating the culturing room of green alga; Described female crustacean receptacle and young crustacean receptacle are the column container of transparent glass material; On the inwall of the bottom tapering part of described young crustacean receptacle, be connected with willow resin bed, described female crustacean receptacle is connected a glass vertical tube with young crustacean receptacle pipe end that be connected and that stretch into one end of young crustacean receptacle, and described glass vertical tube is connected with atmosphere; Described return passage is the column container of transparent, the glass material of bottom taper; Described cultivation matrix apotheca top and return passage are with high.
2. the raising device of crustacean plankton as claimed in claim 1, it is characterized in that, on described female crustacean plankton receptacle C inwall, be connected with the Moschus piece with the ventilative shell of glass, on the ventilative shell of described glass, there are a plurality of small ventilating holes, and be pasted on described female crustacean plankton receptacle C inwall by glue.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410270972.2A CN104026081A (en) | 2014-06-11 | 2014-06-11 | Raising device for crustacean plankton |
| HK15102190.7A HK1201683A1 (en) | 2014-06-11 | 2015-03-04 | Planktonic crustaceans of the feeding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410270972.2A CN104026081A (en) | 2014-06-11 | 2014-06-11 | Raising device for crustacean plankton |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104026081A true CN104026081A (en) | 2014-09-10 |
Family
ID=51457250
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410270972.2A Pending CN104026081A (en) | 2014-06-11 | 2014-06-11 | Raising device for crustacean plankton |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN104026081A (en) |
| HK (1) | HK1201683A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200367476A1 (en) * | 2017-11-29 | 2020-11-26 | Instituto Espanol De Oxceanografea | Method for cultivating paralarvae of the common octopus, octopus vulgaris |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07170886A (en) * | 1993-10-13 | 1995-07-11 | Nippon Suisan Kaisha Ltd | Prawn having salty taste and improved in body color, its heat-treated material and their frozen food |
| CN1440236A (en) * | 2000-07-06 | 2003-09-03 | 长瀬产业株式会社 | Method of culturing zooplankton, apparatus for culturing by the same, and culture obtained by the same |
| RU2292712C1 (en) * | 2005-08-05 | 2007-02-10 | Государственное научное учреждение Научно-исследовательский ветеринарный институт Нечерноземной зоны РФ Российской академии сельскохозяйственных наук | Method for controlling of fasciola miracidium population |
| CN101243781A (en) * | 2008-03-11 | 2008-08-20 | 青岛理工大学 | Zooplankton culture apparatus |
| CN102057888A (en) * | 2010-11-16 | 2011-05-18 | 湖州唐氏渔业科技开发有限公司 | Artificial culturing method and device for large water flea |
| CN102845367A (en) * | 2012-09-26 | 2013-01-02 | 环境保护部南京环境科学研究所 | Automation running-water type cultivation device of daphnia magna |
-
2014
- 2014-06-11 CN CN201410270972.2A patent/CN104026081A/en active Pending
-
2015
- 2015-03-04 HK HK15102190.7A patent/HK1201683A1/en unknown
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07170886A (en) * | 1993-10-13 | 1995-07-11 | Nippon Suisan Kaisha Ltd | Prawn having salty taste and improved in body color, its heat-treated material and their frozen food |
| CN1440236A (en) * | 2000-07-06 | 2003-09-03 | 长瀬产业株式会社 | Method of culturing zooplankton, apparatus for culturing by the same, and culture obtained by the same |
| RU2292712C1 (en) * | 2005-08-05 | 2007-02-10 | Государственное научное учреждение Научно-исследовательский ветеринарный институт Нечерноземной зоны РФ Российской академии сельскохозяйственных наук | Method for controlling of fasciola miracidium population |
| CN101243781A (en) * | 2008-03-11 | 2008-08-20 | 青岛理工大学 | Zooplankton culture apparatus |
| CN102057888A (en) * | 2010-11-16 | 2011-05-18 | 湖州唐氏渔业科技开发有限公司 | Artificial culturing method and device for large water flea |
| CN102845367A (en) * | 2012-09-26 | 2013-01-02 | 环境保护部南京环境科学研究所 | Automation running-water type cultivation device of daphnia magna |
Non-Patent Citations (1)
| Title |
|---|
| 环境保护部化学品登记中心等: "《化学品测试方法 降解蓄积卷》", 30 September 2013, 中国环境出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200367476A1 (en) * | 2017-11-29 | 2020-11-26 | Instituto Espanol De Oxceanografea | Method for cultivating paralarvae of the common octopus, octopus vulgaris |
Also Published As
| Publication number | Publication date |
|---|---|
| HK1201683A1 (en) | 2015-09-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102177867A (en) | Efficient and economical indoor circulating aquaculture system | |
| CN103478055A (en) | Indoor industrial penaeus vannamei culture device | |
| CN110810308A (en) | Intelligent fish and vegetable symbiotic cultivation equipment | |
| CN204907426U (en) | Circulating seed artificial containers | |
| CN102845367B (en) | Automation running-water type cultivation device of daphnia magna | |
| CN204860632U (en) | Case is bred to earthworm | |
| CN206728930U (en) | Recirculated water stereo ecological cultivating system | |
| CN105165729A (en) | Earthworm breeding box | |
| CN211581231U (en) | Hydrologic cycle ecosystem | |
| CN104026081A (en) | Raising device for crustacean plankton | |
| CN211020566U (en) | Container formula circulation aquaculture system | |
| CN202773798U (en) | Daphnia magna automation running form breeding device | |
| CN205196729U (en) | Aquarium fish breeding device | |
| CN209073269U (en) | Aquaculture equipment in a kind of multifunctional room | |
| CN209359243U (en) | Breeding environment moistening and lowering temperature sterilizes integrated control system | |
| CN209218929U (en) | Pond outside a kind of wheel animalcule generator traction | |
| CN208317943U (en) | A kind of fish and vegetable symbiotic system | |
| CN206303011U (en) | A kind of shrimp culture pond of easy blowdown | |
| CN204509070U (en) | Natural pond liquid uniflux promotes that fermentation raw material flows into the efficient methane fermentation system of system | |
| CN204509074U (en) | U-shaped methane fermentation system | |
| CN204509071U (en) | Natural pond liquid uniflux promotes the equally distributed biogas fermentation device of bacterial classification | |
| CN204509067U (en) | Cloth bacterium device promotes bacterial classification equally distributed ultra-high efficiency biogas generation systems | |
| CN215975387U (en) | General type parent fish is cultivated and uses circulating water treatment system | |
| CN204509073U (en) | The two-tube channel-style biogas generating device of hydraulic pressure | |
| CN204509068U (en) | Air pressure promotes that natural pond liquid flowing drives the ultra-high efficiency biogas generation systems of cloth bacterium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1201683 Country of ref document: HK |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140910 |
|
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: WD Ref document number: 1201683 Country of ref document: HK |