CN102057888A - Artificial culturing method and device for large water flea - Google Patents
Artificial culturing method and device for large water flea Download PDFInfo
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- CN102057888A CN102057888A CN 201010545539 CN201010545539A CN102057888A CN 102057888 A CN102057888 A CN 102057888A CN 201010545539 CN201010545539 CN 201010545539 CN 201010545539 A CN201010545539 A CN 201010545539A CN 102057888 A CN102057888 A CN 102057888A
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- daphnia magna
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Abstract
The invention belongs to the field of aquaculture industry and in particular relates to an artificial culturing method and device for large water flea. In the artificial culturing method, the large water flea is cultured by utilizing the culturing waste water of aquatic economical animals. In the culturing process, the temperature is controlled below 25 DEG C and the inoculation density of the large water flea is above 100 pieces per liter. The invention relates to the artificial culturing method and device for the large water flea serving as biological bait. The technical scheme is that the large water flea is cultured by utilizing the culturing waste water of the aquatic economical animals and the bait is thrown in according to the growth condition of the large water flea in the culturing process. Compared with the prior art, the culturing for large water flea by adopting the method provided by the invention has the advantages of low culturing cost, high water use ratio and high quality of large water flea. Besides, the culturing device provided by the invention has the advantage of being simple and convenient in management and convenient for capturing when being used for culturing, and is beneficial to realize industrialized production.
Description
Technical field
The invention belongs to culture fishery, be specifically related to a kind of artificial cultivating method and cultivation equipment of Daphnia magna.
Background technology
Daphnia magna belongs to Arthropoda, Crustachia, Branchiopoda.Daphnia magna is a crustacean plankton common in the Freshwater ecosystems, is constituent important in the fresh water food chain.Because the nutritive value of Daphnia magna is higher, body contains rich in protein and required essential amino acid, the fat of other aquatic animals, also contain abundant vitamin and mineral matter, and have life cycle short, growth is fast, easily cultivate, to characteristics such as poisonous substance sensitivities, therefore some countries are used for growing seedlings of aquatic products economic animal with Daphnia magna, and therefore improved the survival rate of growing seedlings, obtained effect preferably.
Yet the source of Daphnia magna is still to fish at present, and it is less that artificial culture work is carried out; simultaneously existing artificial culture mode exists also that to take coverage of water big, and efficient is low, the cost height; a series of problems such as bait is unholiness, therefore present scale are cultivated and are had certain difficulty.
Summary of the invention
The objective of the invention is to overcome the defective of prior art, a kind of artificial cultivating method of Daphnia magna is provided.
Above-mentioned technical purpose of the present invention is achieved by the following technical programs:
A kind of artificial cultivating method of Daphnia magna utilizes aquatic products economic animal breeding wastewater to carry out the breed of Daphnia magna, and temperature is controlled at below 25 ℃ in the breeding process, more than 100/liter of the inoculum densities of Daphnia magna reach.
Described aquatic products economic animal breeding wastewater is meant that comprising a large amount of fish faeces and bed mud etc. is rich in organic water body.
Preferred as technique scheme, aquatic products economic animal breeding wastewater is through 80 purpose screen filtrations and cultivate little algae.
Described little algae is meant and lives in ocean or the freshwater that body length is no more than 1000 microns unicellular or many cells microphyte.Have chloroplast in their cells, photosynthetic efficiency is far above common plant.They comprise the algae of Bacillariophyta, Chlorophyta, Chrysophyta, Xanthophyta and Cyanophyta.
Preferred as technique scheme, the temperature of described breeding water body is 20 ℃～25 ℃.
Preferred as technique scheme is used for bait that Daphnia magna cultures and is aquatic products with one or more of yeast, rice bran extract, little algae concentrate; Bait is thrown in to once a day, each 3.7～7.5g/ ㎡.
Preferred as technique scheme, in the Daphnia magna breeding process, the beneficial bacterium of need regularly splashing, described beneficial bacterium is selected from one or more in photosynthetic bacteria, the bacillus subtilis; The cycle of splashing of described beneficial bacterium is 10 days; The beneficial bacterium weight of at every turn splashing is 10mg/L.
In the Daphnia magna breeding process, when the density of Daphnia magna is above above 2000/liter, to gather for adult Daphnia magna, concrete picking methods is to use the 35 purpose string bags to gather.
Between picking time, suitably take the sunshade measure, prevent that temperature is too high.
Preferred as technique scheme, when culturing, the depth of water 80～100cm of breeding water body in the control cultivation equipment.
Another object of the present invention provides a kind of cultivation equipment that is used for above-mentioned cultural method.
A kind of cultivation equipment of Daphnia magna comprises the pond body, and Chi Tizhong has set up oxygen-increasing device, and the floor space of described pond body is 1～10 ㎡.
The present invention has following beneficial effect:
The present invention relates to a kind of biological feed--the artificial cultivating method and the equipment of Daphnia magna.This technology mainly utilizes aquatic products economic animal breeding wastewater to raise Daphnia magna, and the growing state according to Daphnia magna during the breed carries out bait throwing in.Compared with prior art, adopt the Daphnia magna that the present invention carried out to culture, have that aquaculture cost is low, a measured advantage of Daphnia magna matter of water body availability height, output.In cultivation equipment provided by the invention, culture in addition and also have simple and convenient management and fish for advantage easily, be beneficial to and realize industrialized production.
Description of drawings
Fig. 1 is the structural representation of Daphnia magna cultivation equipment of the present invention;
Among the figure, 1-pond body, 2-oxygen-increasing device.
Embodiment
Below in conjunction with accompanying drawing the present invention is described in further detail.
This specific embodiment only is an explanation of the invention; it is not a limitation of the present invention; those skilled in the art can make the modification that does not have creative contribution to present embodiment as required after reading this specification, but as long as all are subjected to the protection of Patent Law in claim scope of the present invention.
Embodiment one
Daphnia magna cultivation equipment as shown in fig. 1, this equipment is formed by set up oxygen-increasing device 2 backs in pond body 1, and pond body 1 is a cement pit, and the floor space of cement pit is 6m
2
The cultural method of Daphnia magna specifically comprises the steps:
1) Daphnia magna screening
Screening is healthy, great-hearted Daphnia magna, and removes harmful organisms;
2) processing of aquatic products economic animal breeding wastewater
Filter through 80 eye mesh screens after will being rich in organic breeding wastewater and fully stirring, place Cultivation container to inflate, and give natural lighting, cultivate microalgae;
3) Daphnia magna inoculation
Daphnia magna after the screening is inoculated in the water body that is rich in little algae, and inoculum density is 100/liter;
4) Daphnia magna is cultured
In breeding process, the temperature of breeding water body remains on below 25 ℃, and the depth of water 80～100cm of breeding water body in the control cultivation equipment, the dissolved oxygen in the control breeding water body be at 6mg/L, and promotes water circulation simultaneously;
Growing state according to Daphnia magna carries out, and Daphnia magna is thrown something and fed with yeast with aquatic products, and bait is thrown in to once a day, each 6g/ ㎡;
In the Daphnia magna breeding process, the beneficial bacterium of need regularly splashing, described beneficial bacterium is a photosynthetic bacteria; The cycle of splashing of beneficial bacterium is 10 days; The beneficial bacterium weight of at every turn splashing is 10mg/L;
When the density of Daphnia magna is above above 2000/liter, to gather for adult Daphnia magna, concrete picking methods is to use the 35 purpose string bags to carry out part and gather.
The item that should be noted that:
In inoculation two days later, shine with flashlight at night, then the explanation inoculation is effective in groups for Daphnia magna, connects otherwise need mend; The condition of water quality that the water quality of assurance breeding water body and kind are originated during inoculation differs can not be too big.
Embodiment two
Daphnia magna cultivation equipment as shown in fig. 1, this equipment is formed by set up oxygen-increasing device 2 backs in pond body 1, and pond body 1 is a Glass fibre reinforced plastic tub, and the floor space of Glass fibre reinforced plastic tub is 2m
2
The cultural method of Daphnia magna specifically comprises the steps:
1) Daphnia magna screening
Screening is healthy, great-hearted Daphnia magna, and removes harmful organisms;
2) processing of aquatic products economic animal breeding wastewater
Filter through 80 eye mesh screens after will being rich in organic breeding wastewater and fully stirring, place Cultivation container to inflate, and give natural lighting, cultivate microalgae;
3) Daphnia magna inoculation
Daphnia magna after the screening is inoculated in the water body that is rich in little algae, and inoculum density is 120/liter;
4) Daphnia magna is cultured
In breeding process, the temperature of breeding water body remains on below 25 ℃, and the depth of water 80～100cm of breeding water body in the control cultivation equipment, the dissolved oxygen in the control breeding water body be at 6mg/L, and promotes water circulation simultaneously;
Growing state according to Daphnia magna carries out, and Daphnia magna is thrown something and fed with yeast, rice bran extract, little algae concentrate with aquatic products, and bait is thrown in to once a day, each 6g/ ㎡;
In the Daphnia magna breeding process, the beneficial bacterium of need regularly splashing, described beneficial bacterium is a photosynthetic bacteria; The cycle of splashing of beneficial bacterium is 10 days; The beneficial bacterium weight of at every turn splashing is 10mg/L;
When the density of Daphnia magna is above above 2000/liter, to gather for adult Daphnia magna, concrete picking methods is to use the 35 purpose string bags to carry out part and gather.
The item that should be noted that:
In inoculation two days later, shine with flashlight at night, then the explanation inoculation is effective in groups for Daphnia magna, connects otherwise need mend; The condition of water quality that the water quality of assurance breeding water body and kind are originated during inoculation differs can not be too big.
Claims (10)
1. the artificial cultivating method of a Daphnia magna, it is characterized in that: carry out the breed of Daphnia magna with aquatic products economic animal breeding wastewater as breeding water body, temperature is controlled at below 25 ℃ in the breeding process, 100/liter of the inoculum densities of Daphnia magna and more than.
2. according to the artificial cultivating method of the Daphnia magna described in the claim 1, it is characterized in that: aquatic products economic animal breeding wastewater is through 80 purpose screen filtrations and cultivate little algae.
3. according to the artificial cultivating method of the Daphnia magna described in the claim 1, it is characterized in that: the temperature of described breeding wastewater is 20 ℃ ~ 25 ℃.
4. according to the artificial cultivating method of the Daphnia magna described in the claim 1, it is characterized in that: be used for bait that Daphnia magna cultures and be aquatic products with one or more of yeast, rice bran extract, little algae concentrate; Bait is thrown in to once a day, each 3.7～7.5g/ ㎡.
5. according to the artificial cultivating method of the Daphnia magna described in the claim 1, it is characterized in that: in the Daphnia magna breeding process, the beneficial bacterium of need regularly splashing, described beneficial bacterium is selected from one or more in photosynthetic bacteria, the bacillus subtilis; The cycle of splashing of described beneficial bacterium is 10 days; The beneficial bacterium weight of at every turn splashing is 10mg/L.
6. according to the artificial cultivating method of the Daphnia magna described in the claim 1, it is characterized in that: in the Daphnia magna breeding process, when the density of Daphnia magna is above above 2000/liter, gather for adult Daphnia magna, concrete picking methods is to use the 35 purpose string bags to gather.
7. according to the artificial cultivating method of the Daphnia magna described in the claim 1, it is characterized in that: when culturing, the depth of water 80～100cm of breeding water body in the control cultivation equipment.
8. according to the artificial cultivating method of the Daphnia magna described in the claim 1, it is characterized in that: utilize oxygen increasing equipment when culturing, the dissolved oxygen in the control breeding water body is at 5～7mg/L.
9. the cultivation equipment of Daphnia magna comprises the pond body, and it is characterized in that: Chi Tizhong has set up oxygen-increasing device, and the floor space of described pond body is 1～10 ㎡.
10. the cultivation equipment of Daphnia magna according to claim 9, it is characterized in that: described pond body is cement pit or Glass fibre reinforced plastic tub.
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| CN 201010545539 CN102057888A (en) | 2010-11-16 | 2010-11-16 | Artificial culturing method and device for large water flea |
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| CN 201010545539 CN102057888A (en) | 2010-11-16 | 2010-11-16 | Artificial culturing method and device for large water flea |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103210880A (en) * | 2012-03-21 | 2013-07-24 | 常州市环境监测中心 | Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment |
| CN104026081A (en) * | 2014-06-11 | 2014-09-10 | 温州中普知识产权有限公司 | Raising device for crustacean plankton |
| CN104273101A (en) * | 2014-10-24 | 2015-01-14 | 南京大学 | Artificial cultivation method of Moina micrura Kurz or Ceriodaphnia cornuta Sars |
| CN104488829A (en) * | 2015-01-07 | 2015-04-08 | 董乃畅 | Culture method of moina organism food for aquaculture |
| CN105284740A (en) * | 2015-10-27 | 2016-02-03 | 长江大学 | Method for quickly identifying sex of young daphnia of daphnia magna |
| CN107318748A (en) * | 2017-05-14 | 2017-11-07 | 上海海洋大学 | A kind of Biological control device for aquarium |
| CN108606157A (en) * | 2018-03-26 | 2018-10-02 | 佛山市顺德区活宝源生物科技有限公司 | A kind of bait and preparation method thereof for cultivating zooplankter |
| CN115250977A (en) * | 2022-07-18 | 2022-11-01 | 上海水生科技股份有限公司 | Efficient cultivation and collection system for algae-filtering daphnia for water purification |
-
2010
- 2010-11-16 CN CN 201010545539 patent/CN102057888A/en active Pending
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| 《Manual on the production and use of live food for aquaculture》 19961231 Patrick Lavens, Patrick Sorgeloos 6.1.4 Mass culture of Daphnia Food and Agriculture Organization of the United Nations 285-288 1-8 , * |
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| 《湖北农业科学》 20100630 姜海明 等 芽孢杆菌与光合细菌对乌鳢养殖水的净化作用研究 1428-1430 1-4 第49卷, 第6期 * |
| 《生态学报》 20080930 邓道贵，孟琼，殷四涛，封妮莎，肖琴琴 温度和食物浓度对大型溞Daphnia magna种群动态和两性生殖的影响 4268-4276 6 第28卷, 第9期 * |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103210880A (en) * | 2012-03-21 | 2013-07-24 | 常州市环境监测中心 | Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment |
| CN104026081A (en) * | 2014-06-11 | 2014-09-10 | 温州中普知识产权有限公司 | Raising device for crustacean plankton |
| CN104273101A (en) * | 2014-10-24 | 2015-01-14 | 南京大学 | Artificial cultivation method of Moina micrura Kurz or Ceriodaphnia cornuta Sars |
| CN104488829A (en) * | 2015-01-07 | 2015-04-08 | 董乃畅 | Culture method of moina organism food for aquaculture |
| CN104488829B (en) * | 2015-01-07 | 2016-07-06 | 东营大振生物科技有限公司 | A kind of cultural method of the naked abdomen biological feed for aquaculture |
| CN105284740A (en) * | 2015-10-27 | 2016-02-03 | 长江大学 | Method for quickly identifying sex of young daphnia of daphnia magna |
| CN105284740B (en) * | 2015-10-27 | 2018-03-02 | 长江大学 | Quick discriminating Daphnia magna children's Magna method for distinguishing |
| CN107318748A (en) * | 2017-05-14 | 2017-11-07 | 上海海洋大学 | A kind of Biological control device for aquarium |
| CN108606157A (en) * | 2018-03-26 | 2018-10-02 | 佛山市顺德区活宝源生物科技有限公司 | A kind of bait and preparation method thereof for cultivating zooplankter |
| CN115250977A (en) * | 2022-07-18 | 2022-11-01 | 上海水生科技股份有限公司 | Efficient cultivation and collection system for algae-filtering daphnia for water purification |
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Application publication date: 20110518 |