CN103210880A - Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment - Google Patents
Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment Download PDFInfo
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- CN103210880A CN103210880A CN2012100759431A CN201210075943A CN103210880A CN 103210880 A CN103210880 A CN 103210880A CN 2012100759431 A CN2012100759431 A CN 2012100759431A CN 201210075943 A CN201210075943 A CN 201210075943A CN 103210880 A CN103210880 A CN 103210880A
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Abstract
The invention discloses a device for separating mother daphnia magna and young daphnia magna for a daphnia magna experiment and a method of separating the mother daphnia magna and the young daphnia magna for the daphnia magna experiment. The device comprises a separating tank and separating screens, wherein the separating screens comprise a first separating screen and a second screen which are respectively stuck in the inner wall of the separating tank in a sealing way from top to bottom; the first separating screen is a nylon screen with the pore diameter of 1200 microns; and the second separating screen is a nylon screen with the pore diameter of 1000 microns. According to the device for separating the mother daphnia magna and the young daphnia magna for the daphnia magna experiment, the separating screens which are stuck in the separating tank in a sealing way are adopted, and optimal fineness is selected, so that the mother daphnia magna and the young daphnia magna for the daphnia magna experiment can be separated rapidly, conveniently and cleanly, and the mother daphnia magna and the young daphnia magna can be directly used in a laboratory or on the spot.
Description
Technical field
The present invention relates to a kind of the experiment with biological separator, particularly Daphnia magna is tested separator and the separating method thereof with female Magna and young Magna.
Background technology
Daphnia magna (Daphnia magna) is a kind of biology of living in natural water area, is plankton, zooplankton, Arthropoda, crustacean guiding principle, Branchiopoda, two Leptostraca, dish intestines water Magna Superfamily, water Magna genus.Daphnia magna because of have life cycle short, breeding is fast, economy conveniently is easy to get, and be easy in advantages such as laboratory cultures, and it has consequence in aquatic ecosystem, therefore is subjected to numerous national toxicology workers' attention.Daphnia magna is that susceptibility is stronger, and very representational a kind of zooplankton has now become a kind of code test biology, is widely used in the research of aquatic toxicology.At present, the growth inhibition that relates to Daphnia magna and the standard of breeding are all arranged in iso standard, OECD guide, U.S. EPA standard and China's GB.
The water quality material to the determination of acute toxicity method GB/T 13266-1991 of Magna class (Daphnia magna) in for the following elaboration of separation of the young Magna of birth back 6～24h: before the test from the reserve tube of laboratory the female Magna of 20～30 bosoms of picking ovum, be placed in the 2000mL beaker and cultivate separately.Take female Magna behind the 18h away, young Magna still cultivates in former breeding cylinder, and during 24h, the young Magna in this breeding cylinder is the young Magna of birth 6～24h.For guaranteeing the good breeding of Daphnia magna, adopt following method to separate female Magna: to be sewn into a cup-shaped mesh bag (being slightly less than the breeding cylinder) with aperture 1000 μ m nylon wires and to be enclosed within the breeding inside wall of cylinder, female Magna is put into net, regularly propose to be equipped with the nylon wire of female Magna.Put into the aquarium that another fills the scenedesmus obliquus culture fluid.Also can adopt the liquid that makes the breeding cylinder to obtain test Magna by the upper and lower method that is linked in two screen clothes together.The aperture of upper strata screen cloth is 800～1000 μ m, and the aperture of lower screen is 450～500 μ m.Female Magna is stopped on the coarse net of upper strata, and young Magna is stopped on lower floor's fine-structure mesh.
The weak point of the separating method of above-mentioned GB is: the 1) shelling that female Magna breeding produces to Daphnia magna in the standard method and feed the algae group particle of raising and do not removed, these materials can be attached on one's body the Daphnia magna, cause the motion of Daphnia magna to be affected, thereby have influence on the normal growth and the breeding of Daphnia magna.2) to adopt the method for fit to carry out in the actual mechanical process comparatively loaded down with trivial details in standard method, all will recoil at every turn and clean the screen cloth of different size, and separating rate is slower.3) standard method automaticity is not high enough, can't satisfy the operation of extensive, big flux from now on.
Summary of the invention
First purpose of the present invention provides and a kind ofly can realize quick, convenient, the female Magna of separation of cleaning and the separator of young Magna.
For realizing first purpose of the present invention, basic technology scheme provided by the invention is: the Daphnia magna experiment comprises splitter cylinder and separating screen with the separator of female Magna and young Magna; Described separating screen comprises first separating screen and second separating screen that engages setting from top to bottom respectively with the inner wall sealing of splitter cylinder, and described first separating screen is the nylon screen of 1200 μ m, and described second separating screen is the nylon screen of 1000 μ m.
Based on aforesaid basic technology scheme, two kinds of separators that structure is different have been the present invention further provides.First kind of separator also comprises valve and pipeline on the basis of base apparatus; Described separating screen also comprises the 3rd separating screen that is arranged at second separating screen below and engages setting with the inner wall sealing of splitter cylinder, and described the 3rd separating screen is the nylon screen of 500 μ m; The sidewall that described splitter cylinder is positioned at the 3rd separating screen below is provided with delivery port, on the pipeline that described valve is arranged on delivery port is communicated with.
Second kind of separator also comprises peristaltic pump on the basis of base apparatus; Described peristaltic pump is by pipeline connection mixing Magna liquid reserve tube and splitter cylinder.
Preferred as second kind of separator, the 1L graduation mark on the described splitter cylinder is in the below, position of second separating screen.
Second purpose of the present invention provide a kind of quick, convenient, separate female Magna and young Magna cleanly, be convenient in the laboratory or the on-the-spot separating method that directly uses.
According to aforesaid two kinds of separators that structure is different, the separating method of two kinds of different correspondences is arranged.
Adopt the separating method of first kind of separator, may further comprise the steps:
1. get mixing Magna liquid to be separated in the Magna liquid reserve tube and pour splitter cylinder into from mixing, mix Magna liquid liquid level and need be higher than second separating screen;
2. open the valve draining, make liquid level be lower than second separating screen; Hold back the Magna shell of the female Magna of Daphnia magna on first separating screen and feed the grid algae bulk particle of raising, hold back the female Magna of Daphnia magna on second separating screen;
3. open the valve draining, make liquid level be lower than the 3rd separating screen; Hold back young Magna on the 3rd separating screen.
Adopt the separating method of second kind of separator, concrete steps are: use peristaltic pump according to 8.3cm
2The flow of/s mixed Magna liquid system 1L from mixing to draw in the Magna liquid reserve tube in 2 minutes, will mix Magna liquid by pipeline and be discharged into splitter cylinder equably; Hold back the Magna shell of the female Magna of Daphnia magna on first separating screen and feed the grid algae bulk particle of raising, hold back the female Magna of Daphnia magna on second separating screen, young Magna all enters the bottom of splitter cylinder 4.
After having adopted technique scheme, the present invention has following beneficial effect: (1) separator of the present invention adopts two separating screens that engage the sealing setting with splitter cylinder, and the best fineness of selection, thereby can to the female Magna of Daphnia magna and young Magna realize quick, convenient, separates cleanly, be convenient in the laboratory or on-the-spot directly use.
(2) the present invention's different according to use occasion and scale provide the separator of two kinds of different structures.First kind of separator uses three separating screens to cooperate valve simultaneously, not only realized separating of female Magna and young Magna, and young Magna can be trapped on the separating screen, simultaneously the liquid level of pouring the mixing Magna liquid in the splitter cylinder into is not required, structure is very simple, operate also easily, be fit on a small scale and experimental occasions.And the separator of second kind of structure adopts peristaltic pump to control the volume of the mixing Magna liquid of drawing, and therefore not only accuracy is higher, and separative efficiency also increases substantially.
(3) the respectively corresponding two kinds of separating methods of two kinds of separators of the present invention can be selected to use as required, design ingeniously, easy to implement, are worthy to be popularized.
Description of drawings
Content of the present invention is easier to be expressly understood in order to make, and according to specific embodiment also in conjunction with the accompanying drawings, the present invention is further detailed explanation, wherein below
Fig. 1 is the structural representation of embodiments of the invention 1.
Fig. 2 is the structural representation of embodiments of the invention 2.
Label is in the accompanying drawing:
Embodiment
(embodiment 1)
See Fig. 1, the Daphnia magna experiment of present embodiment comprises splitter cylinder 1, separating screen 2, valve 3 and pipeline 4 with the separator of female Magna and young Magna.Separating screen 2 comprises first separating screen 21, second separating screen 22 and the 3rd separating screen 23 that engages setting from top to bottom respectively with the inner wall sealing of splitter cylinder 1.First separating screen 21 is the nylon screen of 1200 μ m, second separating screen 22 is the nylon screen of 1000 μ m, the 3rd separating screen 23 is the nylon screen of 500 μ m, and these 3 fineness are by testing the optimal separation fineness that draws repeatedly, can guaranteeing the accuracy of separating.The sidewall that splitter cylinder 1 is positioned at the 3rd separating screen 23 belows is provided with delivery port, and valve 3 is arranged on the pipeline 4 that is communicated with delivery port.Various difformities such as that the separator of present embodiment can be done is circular, quadrangle, three separating screens 2 are realized airtight by the bayonet socket and the splitter cylinder 1 of agreeing with.Step by step the liquid in the splitter cylinder is bled off as required by by-pass valve control 3, impurity, female Magna and young Magna are trapped in respectively fast on the separating screen of different fineness and realize separating.
Specifically, the step of the separating method of the separator of use present embodiment:
1. get mixing Magna liquid to be separated in the Magna liquid reserve tube 6 and pour splitter cylinder 1 into from mixing, mix Magna liquid liquid level and need be higher than second separating screen 22.
2. open valve 3 drainings, make liquid level be lower than second separating screen 22; Hold back the Magna shell of the female Magna of Daphnia magna on first separating screen 21 and feed the grid algae bulk particle of raising, first separating screen 21 is taken out to clean up in order to next use.Hold back the female Magna of Daphnia magna on second separating screen 22, second separating screen 22 is taken out rapidly to be put into to mix in the Magna liquid reserve tube 7 continue to cultivate, realize the separation of female Magna.
3. open valve 3 drainings, make liquid level be lower than the 3rd separating screen 23; Hold back young Magna on the 3rd separating screen 23.The 3rd separating screen 23 taken out rapidly with culture fluid wash young Magna, realize the separation of young Magna, young Magna puts into new container then can be directly used in toxotest.
(embodiment 2)
See Fig. 2, the Daphnia magna experiment of present embodiment comprises splitter cylinder 1, separating screen 2 and peristaltic pump 5 with the separator of female Magna and young Magna.Separating screen 2 comprises that engaging first separating screen 21 and second separating screen, 22, the first separating screens 21 that are provided with from top to bottom respectively with the inner wall sealing of splitter cylinder 1 is the nylon screen of 1200 μ m, and second separating screen 22 is the nylon screen of 1000 μ m.Peristaltic pump 5 is communicated with by pipeline 4 and mixes Magna liquid reserve tube 7 and splitter cylinder 1.1L graduation mark on the splitter cylinder 1 is in the below, position of second separating screen 22.The separation principle of this separation device is identical with embodiment's 1, owing to increased peristaltic pump 5, therefore can draw according to quantity automatically and mix Magna liquid, has improved operating efficiency.
When separating, use the flow of peristaltic pump 5 according to 8.3cm2/s, in 2 minutes, mix Magna liquid system 1L from mixing to draw in the Magna liquid reserve tube 6, to mix Magna liquid by pipeline 4 and be discharged into splitter cylinder 1 equably, because the 1L graduation mark on the splitter cylinder 1 is in the below, position of second separating screen 22, therefore mixes the liquid level of Magna liquid below the position of second separating screen 22.Hold back the Magna shell of the female Magna of Daphnia magna on first separating screen 21 and feed the grid algae bulk particle of raising, hold back the female Magna of Daphnia magna on second separating screen 22, young Magna all enters the bottom of splitter cylinder 1.Separate first separating screen 21 to be taken out after finishing cleaning up, second separating screen 22 is taken out rapidly to be put into to mix in the Magna liquid reserve tube 6 continue to cultivate, be used for young Magna that toxotest uses then all in splitter cylinder 1 in order to using next time.
Above-described specific embodiment; purpose of the present invention, technical scheme and beneficial effect are further described; institute is understood that; the above only is specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any modification of being made, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. the Daphnia magna experiment is characterized in that: comprise splitter cylinder (1) and separating screen (2) with the separator of female Magna and young Magna; Described separating screen (2) comprises first separating screen (21) and second separating screen (22) that engages setting from top to bottom respectively with the inner wall sealing of splitter cylinder (1), described first separating screen (21) is the nylon screen of 1200 μ m, and described second separating screen (22) is the nylon screen of 1000 μ m.
2. Daphnia magna experiment according to claim 1 is characterized in that: also comprise valve (3) and pipeline (4) with the separator of female Magna and young Magna; Described separating screen (2) also comprises the 3rd separating screen (23) that is arranged at second separating screen (22) below and engages setting with the inner wall sealing of splitter cylinder (1), and described the 3rd separating screen (23) is the nylon screen of 500 μ m; The sidewall that described splitter cylinder (1) is positioned at the 3rd separating screen (23) below is provided with delivery port, and described valve (3) is arranged on the pipeline (4) that is communicated with delivery port.
3. Daphnia magna experiment according to claim 1 is characterized in that: also comprise peristaltic pump (5) with the separator of female Magna and young Magna; Described peristaltic pump (5) is communicated with by pipeline (4) and mixes Magna liquid reserve tube (6) and splitter cylinder (1).
4. Daphnia magna according to claim 3 is tested the separator with female Magna and young Magna, it is characterized in that: the 1L graduation mark on the described splitter cylinder (1) is in the below, position of second separating screen (22).
5. Daphnia magna according to claim 2 is tested the separating method with the separator of female Magna and young Magna, it is characterized in that may further comprise the steps:
1. get mixing Magna liquid to be separated in the Magna liquid reserve tube (6) and pour splitter cylinder (1) into from mixing, mix Magna liquid liquid level and need be higher than second separating screen (22);
2. open valve (3) draining, make liquid level be lower than second separating screen (22); Hold back the Magna shell of the female Magna of Daphnia magna on first separating screen (21) and feed the grid algae bulk particle of raising, hold back the female Magna of Daphnia magna on second separating screen (22);
3. open valve (3) draining, make liquid level be lower than the 3rd separating screen (23); The 3rd separating screen is held back young Magna on (23).
6. Daphnia magna experiment according to claim 4 is characterized in that: use peristaltic pump (5) according to 8.3cm with the separating method of the separator of female Magna and young Magna
2The flow of/s mixed Magna liquid system 1L from mixing to draw in the Magna liquid reserve tube (6) in 2 minutes, will mix Magna liquid by pipeline (4) and be discharged into splitter cylinder (1) equably; Hold back the Magna shell of the female Magna of Daphnia magna on first separating screen (21) and feed the grid algae bulk particle of raising, hold back the female Magna of Daphnia magna on second separating screen (22), young Magna all enters the bottom of splitter cylinder 4.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012100759431A CN103210880A (en) | 2012-03-21 | 2012-03-21 | Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment |
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| CN2012100759431A CN103210880A (en) | 2012-03-21 | 2012-03-21 | Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment |
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Cited By (4)
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| CN103598150A (en) * | 2013-11-30 | 2014-02-26 | 哈尔滨工业大学 | Rapid cultivation device and method of daphnia magna laboratory |
| CN106359205A (en) * | 2016-09-27 | 2017-02-01 | 大连海洋大学 | Device for living classification of daphnia with phototaxis and classification method |
| CN106771029A (en) * | 2016-12-16 | 2017-05-31 | 常州市环境监测中心 | One kind is used for Daphnia magna acute toxicity detection kit |
| CN106940362A (en) * | 2016-12-16 | 2017-07-11 | 常州市人居环境检测防治中心 | A kind of Portable toxicity test device based on Daphnia magna acute toxicity |
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| CN103598150A (en) * | 2013-11-30 | 2014-02-26 | 哈尔滨工业大学 | Rapid cultivation device and method of daphnia magna laboratory |
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| CN106359205A (en) * | 2016-09-27 | 2017-02-01 | 大连海洋大学 | Device for living classification of daphnia with phototaxis and classification method |
| CN106771029A (en) * | 2016-12-16 | 2017-05-31 | 常州市环境监测中心 | One kind is used for Daphnia magna acute toxicity detection kit |
| CN106940362A (en) * | 2016-12-16 | 2017-07-11 | 常州市人居环境检测防治中心 | A kind of Portable toxicity test device based on Daphnia magna acute toxicity |
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Application publication date: 20130724 |