CN109443878B - Device and method for transferring multiple samples of nerve tissue slice - Google Patents
Device and method for transferring multiple samples of nerve tissue slice Download PDFInfo
- Publication number
- CN109443878B CN109443878B CN201811557824.3A CN201811557824A CN109443878B CN 109443878 B CN109443878 B CN 109443878B CN 201811557824 A CN201811557824 A CN 201811557824A CN 109443878 B CN109443878 B CN 109443878B
- Authority
- CN
- China
- Prior art keywords
- bottle body
- funnel
- nerve tissue
- plug
- tissue slice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000000944 nerve tissue Anatomy 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 239000002699 waste material Substances 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims abstract description 17
- 238000007789 sealing Methods 0.000 claims description 15
- 239000010902 straw Substances 0.000 claims description 6
- 230000005484 gravity Effects 0.000 claims description 5
- 208000005189 Embolism Diseases 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000000717 retained effect Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 210000001519 tissue Anatomy 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 210000000278 spinal cord Anatomy 0.000 description 2
- 244000287353 Crassocephalum crepidioides Species 0.000 description 1
- 206010016322 Feeling abnormal Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the field of neurobiology, and particularly relates to a device and a method for transferring multiple samples of nerve tissue slices, wherein the device for transferring multiple samples of nerve tissue slices comprises a negative pressure device, a filtering device and a suction device; the negative pressure device comprises a bottle body and a plug, wherein the bottle body is hollow and cylindrical, and two ends of the bottle body are open; the plug is arranged in the bottle body, the plug and the bottle body are sealed, and the plug can slide along the bottle body; the filtering device comprises a funnel and a filter screen, wherein a big port of the funnel is detachably arranged at one end of the bottle body and is sealed with the bottle body; the filter screen covers the big port of the funnel; the suction device comprises a suction pipe which is connected with the small port of the funnel. The device for transferring the nerve tissue slice multiple samples has compact structure, simple and stable operation, can transfer multiple samples at one time, does not contain waste liquid, and greatly improves the efficiency of the operations of replacing the nerve tissue slice, incubating antibodies and the like of experimental researchers.
Description
Technical Field
The invention belongs to the field of neurobiology, and particularly relates to a device and a method for transferring multiple samples of a nerve tissue slice.
Background
Nerve tissue sections are typically derived from the brain and spinal cord of rodents, and are one type of specimen that is widely used in the field of neuroscience research, including various slice types such as frozen slices, paraffin sections, golgi sections, ultra-thin sections, and the like. The brain or spinal cord tissue after being lavaged by PBS or fixed by paraformaldehyde is completely peeled from an animal body, soaked in Golgi solution or dehydrated by sucrose, and then tissue specimens with different thicknesses of 20-200 μm are obtained in a microtome according to experimental requirements, so that corresponding experimental treatment is carried out.
Different nerve tissue sections have different textures, and the experimental treatment often needs to be performed by selecting a transfer tool according to the tissue section properties so as to avoid unnecessary tissue damage, and meanwhile, the experimental treatment also needs to be performed by transferring multiple samples so as to improve the experimental efficiency. At present, the laboratory often adopts the following modes to transfer the slices: if a metal fishing hook is used for transferring tissue slices, firstly, the time consumption is long and the number of samples which can be transferred is limited, and secondly, the tissue is often broken due to the gravity of water when the Golgi slices with brittle textures are transferred; if a large amount of samples can be transferred at one time by using the thick-head straw, a corresponding amount of waste liquid can be brought to influence the subsequent experimental treatment; if the filter plate transfer slice is used, only simple storage and liquid exchange treatment can be performed, and transfer treatment between experimental facilities with different sizes, such as antibody incubation and the like, cannot be performed. The method is low in efficiency, time-consuming and labor-consuming in the whole transfer process, and multiple sample transfer operation of multiple types of nerve tissue slices cannot be realized. Therefore, it is necessary to design a device that can transfer a large number of nerve tissue sections at a time and also can ensure that the transfer process does not have too much waste liquid.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a device and a method for transferring multiple samples of nerve tissue slices, which can transfer a large number of nerve slices at one time and ensure that no waste liquid is contained in the transfer process.
In order to solve the technical problems, the invention adopts the following technical scheme:
the device for transferring the multiple samples of the nerve tissue slice comprises a negative pressure device, a filtering device and a suction device;
the negative pressure device comprises a bottle body and a plug, wherein the bottle body is hollow and cylindrical, and two ends of the bottle body are open; the plug is arranged in the bottle body, the plug and the bottle body are sealed, and the plug can slide along the bottle body;
the filtering device comprises a funnel and a filter screen, wherein a big port of the funnel is detachably arranged at one end of the bottle body and is sealed with the bottle body; the filter screen covers the big port of the funnel;
the suction device comprises a suction pipe which is connected with the small port of the funnel.
The large port of the funnel is connected with an external thread ring, and the external thread ring is of a circular ring structure with external threads on the outer surface; the inner surface of the end part of one end of the bottle body connected with the funnel is provided with an internal thread matched with the external thread ring;
the big port of funnel stretches into in the body, and the outer edge of big port of funnel has inlayed rubber sealing ring, and the funnel passes through rubber sealing ring and body seal.
The port of body one end is equipped with the spacing draw-in groove that is used for spacing to the embolism.
The plug is a rubber plug or a hard plug; when the plug is a hard plug, a sealing ring used for forming sealing with the bottle body is sleeved on the contact surface of the plug and the bottle body.
The end of the suction pipe is connected with a shovel-shaped suction head.
The filter screen is a flexible filter screen.
The aperture of the filter screen is 1-1.5 mm.
The suction tube is a flexible tube, and the inner surface of the suction tube is coated with an anti-sticking material.
A method for transferring multiple samples of nerve tissue slices, which is carried out by adopting the device for transferring multiple samples of nerve tissue slices, and comprises the following steps:
s1: pulling the embolism, and sucking the nerve tissue slice to be transferred into the funnel from the suction pipe;
s2: the bottle body is turned upside down, so that the big port of the funnel faces downwards, waste liquid mixed in the nerve tissue slice is filtered by a filter screen under the action of gravity, and the nerve tissue slice is retained in the funnel;
s3: separating the funnel from the bottle body after filtering the waste liquid mixed in the nerve tissue slice, then replacing clean new liquid into the bottle body, and then installing the funnel and the bottle body together; or separating the funnel from the bottle body, then replacing clean new liquid into the bottle body, and installing the funnel and the bottle body together after filtering the waste liquid mixed in the nerve tissue slice;
s4: pushing the plug, filling clean new liquid into the bottle body from the filter screen into the funnel, washing the nerve tissue slice in the funnel and discharging the nerve tissue slice along the straw.
The invention has the following beneficial effects:
the plug of the device for transferring the nerve tissue slice multiple samples is sealed with the bottle body, the plug can slide along the bottle body, and the large port of the funnel is detachably arranged at one end of the bottle body and is sealed with the bottle body; the filter screen covers the big port of the funnel; the suction pipe is connected with the small port of the funnel; when the nerve tissue slice is transferred, the plug is lifted to enable the inside of the bottle body to generate negative pressure, and the generated negative pressure sucks the nerve tissue slice to be transferred into the funnel from the suction pipe; after the suction is finished, the bottle body is turned upside down, so that the big port of the funnel faces downwards, and the waste liquid mixed in the nerve tissue slice is filtered out by the filter screen under the action of gravity, so that the nerve tissue slice stays in the funnel; then: separating the funnel from the bottle body after filtering the waste liquid mixed in the nerve tissue slice, then replacing clean new liquid into the bottle body, and then installing the funnel and the bottle body together; or separating the funnel from the bottle body, then replacing clean new liquid into the bottle body, and installing the funnel and the bottle body together after filtering the waste liquid mixed in the nerve tissue slice; and finally pushing the plug, and enabling clean new liquid filled in the bottle body to enter the funnel from the filter screen, washing the nerve tissue slice in the funnel and discharging the nerve tissue slice along the straw. The device for transferring the nerve tissue slice multiple samples has compact structure and simple and stable operation, can transfer the multiple samples at one time, does not contain waste liquid, and greatly improves the efficiency of the operations of replacing the nerve tissue slice, incubating antibodies and the like of experimental researchers.
The device for transferring the nerve tissue slice multiple samples has the beneficial effects that the method for transferring the nerve tissue slice multiple samples is simple and stable in operation, can transfer multiple samples at one time and does not contain waste liquid, and the efficiency of operations such as liquid exchange and antibody incubation of the nerve tissue slice by experimental researchers is greatly improved.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a multiple sample transfer apparatus for nerve tissue slices according to the present invention;
FIG. 2 is a schematic structural view of the negative pressure mounting device of the present invention;
FIG. 3 is a schematic view of the structure of the filtering device and the externally threaded ring according to the present invention;
FIG. 4 is a schematic view of the structure of the suction pipe device of the present invention.
In the figure, the bottle comprises a 1-limit clamping groove, a 2-plug, a 3-bottle body, a 4-internal thread, a 6-filter screen, a 7-rubber sealing ring, an 8-funnel, a 9-external thread ring, a 10-suction pipe and an 11-shovel-shaped suction head.
Detailed Description
The invention will be further described with reference to the drawings and examples.
Referring to fig. 1, the apparatus for multi-sample transfer of a nerve tissue slice of the present invention includes a negative pressure device, a filtering device, and a suction device; the negative pressure device comprises a bottle body 3 and a plug 2, wherein the bottle body 3 is in a hollow cylinder shape with two open ends; the plug 2 is arranged in the bottle body 3, the plug 2 and the bottle body 3 are sealed, and the plug 2 can slide along the bottle body 3; the filtering device comprises a funnel 8 and a filter screen 6, wherein a large port of the funnel 8 is detachably arranged at one end of the bottle body 3 and is sealed with the bottle body 3; the filter screen 6 covers the big port of the funnel 8; the suction device comprises a suction pipe 10, and the suction pipe 10 is connected with a small port of the funnel 8.
As shown in fig. 3, referring to fig. 1 and 2, as a preferred embodiment of the present invention, the large port of the funnel 8 is connected with an externally threaded ring 9, and the externally threaded ring 9 has a circular ring structure with external threads on the outer surface; the end part of one end of the bottle body 3 connected with the funnel 8 is provided with an internal thread 4 matched with an external thread ring 9 on the inner surface; the big port of the funnel 8 stretches into the bottle body 3, the outer edge of the big port of the funnel 8 is embedded with a rubber sealing ring 7, and the funnel 8 is sealed with the bottle body 3 through the rubber sealing ring 7. During the use, through the cooperation connection between the external screw thread on the external screw thread ring 9 and the internal screw thread of body 3 lower extreme for funnel 8 realize with the body 3 between can closely laminate, realize the seal between funnel 8 and the body 3 between the internal face of rubber seal ring 7 and body 3 simultaneously, consequently can produce the negative pressure at the inner chamber of body 3 when upwards pulling up to plug 2, the negative pressure that produces can inhale the funnel 8 with the nerve tissue section that needs to shift from straw 10 in.
As shown in fig. 1 and 2, as a preferred embodiment of the present invention, a limit slot 1 for limiting the plug 2 is provided at a port at the upper end of the body 3, and the limit slot 1 can prevent the plug 2 from being pulled out from the upper port of the body 3 during the pulling process.
As a preferred embodiment of the invention, the plug 2 is a rubber plug, or a hard plug; when the plug 2 is a hard plug, a sealing ring used for forming sealing with the bottle body 3 is sleeved on the contact surface of the plug 2 with the bottle body 3, and sealing between the plug 2 and the bottle body 3 and sliding of the plug 2 along the bottle body 3 can be realized through sealing connection of the two types.
As a preferred embodiment of the present invention, a shovel tip 11 is attached to the end of the pipette 10 to facilitate the aspiration of samples in corners.
As a preferred embodiment of the present invention, the screen 6 is flexible and is capable of deforming because it is flexible, which can easily separate the nerve tissue slice from the screen 6 during pushing of the plug 2 to expel the nerve tissue slice along the straw 10.
As a preferred embodiment of the present invention, the pore size of the sieve 6 is 1 to 1.5mm.
As a preferred embodiment of the present invention, the suction tube 10 is a flexible tube with an interior surface coated with a release material that prevents damage to the nerve tissue slice by bonding to the interior wall of the suction tube 10.
The process of transferring the nerve tissue slice multiple samples by adopting the device for transferring the nerve tissue slice multiple samples comprises the following steps:
s1: pulling the plug 2, and sucking the nerve tissue slice to be transferred from the suction tube 10 into the funnel 8;
s2: the bottle body 3 is turned upside down, so that the big port of the funnel 8 faces downwards, waste liquid mixed in the nerve tissue slice is filtered out by the filter screen 6 under the action of gravity, and the nerve tissue slice is retained in the funnel 8;
s3: after filtering the waste liquid mixed in the nerve tissue slice, separating the funnel 8 from the bottle body 3, then replacing clean new liquid into the bottle body 3, and then installing the funnel 8 and the bottle body 3 together; or separating the funnel 8 from the bottle body 3, then replacing clean new liquid into the bottle body 3, and installing the funnel 8 and the bottle body 3 together after filtering the waste liquid mixed in the nerve tissue slice;
the plug 2 is pushed, clean new liquid filled in the bottle body 3 enters the funnel 8 from the filter screen 6, and nerve tissue slices in the funnel 8 are rinsed and discharged along the suction pipe 10.
Claims (8)
1. A method for multi-sample transfer of a nerve tissue slice, characterized in that the method is carried out by adopting a device for multi-sample transfer of the nerve tissue slice, wherein the device for multi-sample transfer of the nerve tissue slice comprises a negative pressure device, a filtering device and a suction device;
the negative pressure device comprises a bottle body (3) and a plug (2), wherein the bottle body (3) is in a hollow cylinder shape with two open ends; the plug (2) is arranged in the bottle body (3), the plug (2) is sealed with the bottle body (3), and the plug (2) can slide along the bottle body (3);
the filtering device comprises a funnel (8) and a filter screen (6), wherein a large port of the funnel (8) is detachably arranged at one end of the bottle body (3) and is sealed with the bottle body (3); the filter screen (6) covers the big port of the funnel (8);
the suction device comprises a suction pipe (10), and the suction pipe (10) is connected with a small port of the funnel (8);
the process of the method is as follows:
s1: pulling the plug (2), and sucking the nerve tissue slice to be transferred from the suction tube (10) into the funnel (8);
s2: the bottle body (3) is turned upside down, so that the big port of the funnel (8) faces downwards, waste liquid mixed in the nerve tissue slice is filtered out by the filter screen (6) under the action of gravity, and the nerve tissue slice is retained in the funnel (8);
s3: separating the funnel (8) from the bottle body (3) after filtering the waste liquid mixed in the nerve tissue slice, then replacing clean new liquid into the bottle body (3), and then installing the funnel (8) and the bottle body (3) together; or separating the funnel (8) from the bottle body (3), then replacing clean new liquid into the bottle body (3), and installing the funnel (8) and the bottle body (3) after filtering the waste liquid mixed in the nerve tissue slice;
s4: pushing the plug (2), and enabling clean new liquid filled in the bottle body (3) to enter the funnel (8) from the filter screen (6), washing the nerve tissue slice in the funnel (8) and discharging the nerve tissue slice along the straw (10).
2. The method for multi-sample transfer of nerve tissue sections according to claim 1, characterized in that the big port of the funnel (8) is connected with an externally threaded ring (9), and the externally threaded ring (9) is a circular ring structure with external threads on the outer surface;
an inner surface of the end part of one end of the bottle body (3) connected with the funnel (8) is provided with an inner thread (4) matched with an outer thread ring (9);
the big port of the funnel (8) stretches into the bottle body (3), the outer edge of the big port of the funnel (8) is embedded with a rubber sealing ring (7), and the funnel (8) is sealed with the bottle body (3) through the rubber sealing ring (7).
3. The method for transferring multiple samples of nerve tissue slices according to claim 1, wherein a limiting clamping groove (1) for limiting the embolism (2) is arranged at a port at one end of the bottle body (3).
4. A method for multiple sample transfer of nerve tissue sections according to claim 1, characterized in that the plug (2) is a rubber plug or a hard plug; when the plug (2) is a hard plug, a sealing ring used for forming sealing with the bottle body (3) is sleeved on the contact surface of the plug (2) with the bottle body (3).
5. A method for multiple sample transfer of nerve tissue sections according to claim 1, characterized in that the end of the suction tube (10) is connected with a spade tip (11).
6. A method for multiple sample transfer of nerve tissue sections according to claim 1, characterized in that the screen (6) is a flexible screen.
7. A method for multiple sample transfer of nerve tissue sections according to claim 1, characterized in that the mesh size of the screen (6) is 1-1.5 mm.
8. A method for multiple sample transfer of nerve tissue sections according to claim 1, characterized in that the suction tube (10) is a flexible tube, the inner surface of which is coated with a release material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811557824.3A CN109443878B (en) | 2018-12-19 | 2018-12-19 | Device and method for transferring multiple samples of nerve tissue slice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811557824.3A CN109443878B (en) | 2018-12-19 | 2018-12-19 | Device and method for transferring multiple samples of nerve tissue slice |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109443878A CN109443878A (en) | 2019-03-08 |
| CN109443878B true CN109443878B (en) | 2023-11-10 |
Family
ID=65560306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811557824.3A Active CN109443878B (en) | 2018-12-19 | 2018-12-19 | Device and method for transferring multiple samples of nerve tissue slice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109443878B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113040840B (en) * | 2021-02-23 | 2022-06-21 | 长沙众智医疗器械有限责任公司 | Nerve guiding device |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102759472A (en) * | 2011-04-20 | 2012-10-31 | 徕卡生物系统努斯洛克有限公司 | Tissue sample handling apparatus |
| US9322748B1 (en) * | 2014-09-16 | 2016-04-26 | II Melbourne Kimsey | Tissue collection and refining device and method |
| CN205286416U (en) * | 2015-12-10 | 2016-06-08 | 中国人民解放军第四军医大学 | Can separate device of retrieving brain tumor tissue |
| CN206081166U (en) * | 2016-07-26 | 2017-04-12 | 南京市胸科医院 | Negative -pressure drainage device |
| CN107308706A (en) * | 2017-07-19 | 2017-11-03 | 中国石油天然气股份有限公司 | Positive/negative-pressure laboratory water analysis sample Multistage filtering device |
| CN107812451A (en) * | 2017-11-12 | 2018-03-20 | 孟煜凯 | A kind of hand suction type medicine detection separating and filtering machine people |
| CN108348266A (en) * | 2015-11-25 | 2018-07-31 | 史密夫和内修有限公司 | Tissue grabber for cartilage autograft transfer system |
| CN208223846U (en) * | 2018-04-10 | 2018-12-11 | 徐璐 | A kind of oncology laboratory sampling device |
| CN209342476U (en) * | 2018-12-19 | 2019-09-03 | 中国人民解放军第四军医大学 | A kind of device for the transfer of neural tissue slice multisample |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112014029655A2 (en) * | 2012-05-30 | 2017-06-27 | Lifecell Corp | tissue preparation device, and method for preparing tissue |
| AU2016219956B2 (en) * | 2015-02-19 | 2020-08-13 | Lifecell Corporation | Tissue processing device and associated systems and methods |
-
2018
- 2018-12-19 CN CN201811557824.3A patent/CN109443878B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102759472A (en) * | 2011-04-20 | 2012-10-31 | 徕卡生物系统努斯洛克有限公司 | Tissue sample handling apparatus |
| US9322748B1 (en) * | 2014-09-16 | 2016-04-26 | II Melbourne Kimsey | Tissue collection and refining device and method |
| CN108348266A (en) * | 2015-11-25 | 2018-07-31 | 史密夫和内修有限公司 | Tissue grabber for cartilage autograft transfer system |
| CN205286416U (en) * | 2015-12-10 | 2016-06-08 | 中国人民解放军第四军医大学 | Can separate device of retrieving brain tumor tissue |
| CN206081166U (en) * | 2016-07-26 | 2017-04-12 | 南京市胸科医院 | Negative -pressure drainage device |
| CN107308706A (en) * | 2017-07-19 | 2017-11-03 | 中国石油天然气股份有限公司 | Positive/negative-pressure laboratory water analysis sample Multistage filtering device |
| CN107812451A (en) * | 2017-11-12 | 2018-03-20 | 孟煜凯 | A kind of hand suction type medicine detection separating and filtering machine people |
| CN208223846U (en) * | 2018-04-10 | 2018-12-11 | 徐璐 | A kind of oncology laboratory sampling device |
| CN209342476U (en) * | 2018-12-19 | 2019-09-03 | 中国人民解放军第四军医大学 | A kind of device for the transfer of neural tissue slice multisample |
Non-Patent Citations (1)
| Title |
|---|
| 抽吸负压对所抽吸脂肪颗粒活性影响的研究;雷华 等;《中国美容医学》;第14卷(第1期);第27-30页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109443878A (en) | 2019-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106644663A (en) | Filter apparatus and count method for counting porphyra spores | |
| CN108330057B (en) | Intestinal content flora extraction device and extraction method | |
| CN205079992U (en) | Liquid feeding device of centrifugal liquid base cell film -making | |
| CN109443878B (en) | Device and method for transferring multiple samples of nerve tissue slice | |
| CN202330073U (en) | Medicine dissolving-out sampler | |
| CN113533006A (en) | Full-automatic dyeing machine capable of quickly preparing slices | |
| CN209342476U (en) | A kind of device for the transfer of neural tissue slice multisample | |
| CN104931316A (en) | Cell preserving and centrifugal filtering device | |
| CN217845704U (en) | Multilayer sampling device for sludge | |
| JPH0862210A (en) | Liquid specimen cell-smearing apparatus and its using method | |
| CN210128871U (en) | Device for taking loose stool sample | |
| CN215262692U (en) | Sheet making device | |
| CN207081582U (en) | A kind of sampler for being used to test water body chlorophyll | |
| KR100406430B1 (en) | Density concentrating device for examining the aquatic microalgae | |
| CN206258298U (en) | One main laver spore count filter | |
| CN219377197U (en) | Preservation tube for filtering plug in sample | |
| CN219422857U (en) | Semen collection specimen cup | |
| CN208553291U (en) | A kind of visual box sand core Suction filtration device | |
| CN210103942U (en) | Device for conveniently collecting exfoliated cells in large amount of body fluid | |
| KR200342648Y1 (en) | Filtering apparatus of testing specimen for preparing slide | |
| CN217738796U (en) | Liquid-based thin-layer cell film-making dyeing device | |
| CN112871473B (en) | Quick film-making subassembly suitable for horizontal centrifuge | |
| CN219323092U (en) | Limnoperna lacustris larva collection device | |
| CN219897251U (en) | Human tissue filter equipment | |
| CN220340191U (en) | Portable water quality detector for fish culture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |