CN209342476U - A kind of device for the transfer of neural tissue slice multisample - Google Patents
A kind of device for the transfer of neural tissue slice multisample Download PDFInfo
- Publication number
- CN209342476U CN209342476U CN201822150106.6U CN201822150106U CN209342476U CN 209342476 U CN209342476 U CN 209342476U CN 201822150106 U CN201822150106 U CN 201822150106U CN 209342476 U CN209342476 U CN 209342476U
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- China
- Prior art keywords
- embolism
- funnel
- neural tissue
- multisample
- tissue slice
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Abstract
The utility model belongs to Neurobiology field, and in particular to a kind of device for the transfer of neural tissue slice multisample, including negative pressure device, filter device and suction means；Negative pressure device includes body and embolism, and body is the hollow tubular of both ends open；Embolism is set in body, seals between embolism and body and embolism can be slided along body；Filter device includes funnel and strainer, and the large port of funnel is removably mounted to one end of body and seals between body；Strainer is covered in the large port of funnel；Suction means includes suction pipe, and suction pipe is connect with funnel portlet.The utility model for the transfer of neural tissue slice multisample apparatus structure is compact, stabilization easy to operate, can disposably shift multisample and without waste liquid, greatly improve experimental study personnel and carry out the efficiency that neural tissue slice changes liquid, is incubated for the operations such as antibody.
Description
Technical field
The utility model belongs to Neurobiology field, and in particular to it is a kind of for neural tissue slice multisample transfer
Device.
Background technique
Neural tissue slice is typically derived from the brain and spinal cord of rodent, is answered extensively in Neuroscience Research field
A variety of slice types such as a kind of specimen types, including frozen section, paraffin section, Gorky's slice, ultra-thin section.With
Brain or myeloid tissue of the PBS lavation after perhaps paraformaldehyde is fixed completely stripped out out of animal body steep into Gorky's liquid or
After sucrose dehydration, need to obtain the tissue specimen that 20 μm of -200 μ m thick does not wait in slicer according to experiment, from
And carry out corresponding experiment process.
Different neural tissue slice quality are different, carry out generally requiring to carry out for its histotomy property when experiment process
Select the transfer tool to avoid unnecessary tissue damage, while we are also required to carry out the transfer of multisample to improve experiment
Efficiency.Laboratory often takes following methods to carry out slice transfer at present: if shifting histotomy using metal crowfoot, first is that
The limited sample size that the time is longer and can shift is expended, second is that shifting the gravity that the more crisp Gorky of quality is sliced Shi Yinshui
It usually will cause tissue fracture；Though also bringing along corresponding amount simultaneously if can disposably shift great amount of samples using thick head straw
Waste liquid influence subsequent experimental processing；It is sliced if being shifted using filter plate, only can be carried out simple storage and changes liquid processing, it cannot
Carry out the operation such as transfer processing between various sizes of Experimental Establishment, such as antibody incubation.The above method causes entirely to shift
Process efficiency is low, time and effort consuming, cannot achieve the multisample transfer operation of the neural tissue slice of polymorphic type.Therefore, it is necessary to
Design it is a kind of can once shift a large amount of neural tissue slices, and can guarantee that transfer process does not have the device of too many waste liquid.
Utility model content
In view of the deficienciess of the prior art, the purpose of this utility model is to provide one kind for neural tissue slice it is more
The device of sample transfer can disposably shift a large amount of nerve slices by the device of the utility model, and guarantee to shift
Waste liquid is not contained in journey.
In order to solve the above-mentioned technical problem, the utility model adopts the following technical solution is achieved:
A kind of device for the transfer of neural tissue slice multisample, including negative pressure device, filter device and suction means；
Negative pressure device includes body and embolism, and body is the hollow tubular of both ends open；Embolism is set in body, embolism
It is sealed between body and embolism can be slided along body；
Filter device includes funnel and strainer, and the large port of funnel is removably mounted to one end of body and between body
Sealing；Strainer is covered in the large port of funnel；
Suction means includes suction pipe, and suction pipe is connect with funnel portlet.
The large port of funnel is connected with external screw thread ring, and external screw thread ring is that outer surface has externally threaded circular ring structure；Body
The end of upper one end being connected with funnel is provided with the internal screw thread with the cooperation of external screw thread ring in inner surface；
The large port of funnel protrudes into body, and the outer of funnel large port is embedded with rubber seal ring, and funnel is close by rubber
Seal ring and body seal.
The port of body one end is equipped with the limit card slot for limiting to embolism.
Embolism is rubber embolism, or is hard embolism；When embolism is hard embolism, in the contact with body on embolism
The sealing ring for forming sealing between body is arranged on face.
The end of suction pipe is connected with spade suction nozzle.
Strainer is flexible filter screen.
The aperture of strainer is 1~1.5mm.
Suction pipe is flexible pipe, and inner surface is coated with adhesive.
The utility model has the following beneficial effects:
Sealing and embolism between the device embolism and body for the transfer of neural tissue slice multisample of the utility model
It can be slided along body, the large port of funnel is removably mounted to one end of body and seals between body；Strainer is covered in
The large port of funnel；Suction pipe is connect with funnel portlet；When shifting neural tissue slice, lifting embolism can make inside body
Negative pressure is generated, the negative pressure of generation is by the neural tissue slice of required transfer from suction pipe suction funnel；It sucks and then by body
It flips upside down, makes the big end mouth down of funnel, the waste liquid being mixed in neural tissue slice at this time is passed through strainer by gravity
It filters out, neural tissue slice is trapped in funnel；Then: after the waste liquid wait be mixed in neural tissue slice filters out, by funnel
It is separated with body, then changes to clean new liquid into body, be then installed together funnel and body；Alternatively, first by funnel
It is separated with body, then changes to clean new liquid into body, after the waste liquid wait be mixed in neural tissue slice filters out, then will leakage
Bucket is installed together with body；Embolism is finally pushed, the clean new liquid being packed into body enters funnel from strainer, washes away in funnel
Neural tissue slice and make neural tissue slice along suction pipe be discharged.The utility model is shifted for neural tissue slice multisample
Apparatus structure is compact, stabilization easy to operate, can disposably shift multisample and without waste liquid, greatly improve experimental study
Personnel carry out the efficiency that neural tissue slice changes liquid, is incubated for the operations such as antibody.
Detailed description of the invention
Fig. 1 is the overall structure diagram that the utility model is used for neural tissue slice multisample transfer device；
Fig. 2 is the structural schematic diagram of the utility model negative pressure assembling device；
Fig. 3 is the structural schematic diagram of the utility model filter device and external screw thread ring；
Fig. 4 is the structural schematic diagram of the utility model suction tube device.
In figure, 1- limit card slot, 2- embolism, 3- body, 4- internal screw thread, 6- strainer, 7- rubber seal ring, 8- funnel, 9-
External screw thread ring, 10- suction pipe, 11- spade suction nozzle.
Specific embodiment
The utility model is further described with reference to the accompanying drawings and examples.
Referring to Fig.1, the device for the transfer of neural tissue slice multisample of the utility model, including negative pressure device, mistake
Filter device and suction means；Wherein, negative pressure device includes body 3 and embolism 2, and body 3 is the hollow tubular of both ends open；Embolism
2 are set in body 3, seal between embolism 2 and body 3 and embolism 2 can be slided along body 3；Filter device includes 8 He of funnel
Strainer 6, the large port of funnel 8 are removably mounted to one end of body 3 and seal between body 3；Strainer 6 is covered in funnel 8
Large port；Suction means includes suction pipe 10, and suction pipe 10 is connect with 8 portlet of funnel.
As shown in figure 3, referring to Figures 1 and 2, as the utility model preferred embodiment, the large port of funnel 8 is connected
There is external screw thread ring 9, external screw thread ring 9 is that outer surface has externally threaded circular ring structure；The one end being connected on body 3 with funnel 8
End is provided with the internal screw thread 4 cooperated with external screw thread ring 9 in inner surface；The large port of funnel 8 protrudes into body 3,8 big end of funnel
The outer of mouth is embedded with rubber seal ring 7, and funnel 8 is sealed by rubber seal ring 7 and body 3.When in use, by by external screw thread
Mating connection between the internal screw thread of 3 lower end of external screw thread on ring 9 and body, so that removable between the realization of funnel 8 and body 3
Connection is unloaded, while can be fitted closely between rubber seal ring 7 and the inner wall of body 3, is realized between funnel 8 and body 3
Sealing, therefore negative pressure can be generated in the inner cavity of body 3 when lifting embolism 2 upwards, the negative pressure of generation can be by required transfer
Neural tissue slice from 10 suction funnel 8 of suction pipe.
As depicted in figs. 1 and 2, as the utility model preferred embodiment, the port of 3 upper end of body be equipped with for pair
The limit card slot 1 that embolism 2 limits, limit card slot 1 can prevent embolism 2 from deviating from during lifting from 3 upper port of body.
As the utility model preferred embodiment, embolism 2 is rubber embolism, or is hard embolism；When embolism 2 is
The sealing ring for forming sealing between body 3 is arranged with when hard embolism, on embolism 2 on the contact surface of body 3, is led to
The sealed connection of both forms is crossed, the sealing and embolism 2 that can be realized between embolism 2 and body 3 can be slided along body 3
It is dynamic.
As the utility model preferred embodiment, the end of suction pipe 10 is connected with spade suction nozzle 11, convenient for drawing angle
Sample in falling.
As the utility model preferred embodiment, strainer 6 is flexible filter screen, since strainer is flexible, energy
It enough deforms, during pushing embolism 2 that neural tissue slice is discharged along suction pipe 10, the deformation of flexible filter screen can be easy
In making to separate between neural tissue slice and strainer 6.
As the utility model preferred embodiment, the aperture of strainer 6 is 1~1.5mm.
As the utility model preferred embodiment, suction pipe 10 is flexible pipe, and inner surface is coated with adhesive, anti-sticking material
Material can prevent neural tissue slice Nian Jie with suction pipe 10 inner wall generation and cause to damage.
Neural tissue slice multisample is shifted using the device that the utility model neural tissue slice multisample shifts
Process is as follows:
S1: lifting embolism 2, by the neural tissue slice of required transfer from 10 suction funnel 8 of suction pipe；
S2: body 3 is flipped upside down, and makes the big end mouth down of funnel 8, is mixed in the waste liquid in neural tissue slice by weight
Power acts through strainer 6 and filters out, and neural tissue slice is trapped in funnel 8；
S3: after the waste liquid wait be mixed in neural tissue slice filters out, funnel 8 is separated with body 3, then into body 3
Funnel 8 and body 3, are then installed together by the clean new liquid of change；Alternatively, first funnel 8 is separated with body 3, then to bottle
Clean new liquid is changed in body 3, after the waste liquid wait be mixed in neural tissue slice filters out, then funnel 8 and body 3 is mounted on
Together；
Embolism 2 is pushed, the clean new liquid being packed into body 3 enters funnel 8 from strainer 6, washes away the nerve fiber in funnel 8
It is sliced and neural tissue slice is discharged along suction pipe 10.
Claims (8)
1. it is a kind of for neural tissue slice multisample transfer device, which is characterized in that including negative pressure device, filter device and
Suction means；
Negative pressure device includes body (3) and embolism (2), and body (3) is the hollow tubular of both ends open；Embolism (2) is set to bottle
In body (3), is sealed between embolism (2) and body (3) and embolism (2) can be slided along body (3)；
Filter device includes funnel (8) and strainer (6), the large port of funnel (8) be removably mounted to one end of body (3) and with
Body seals between (3)；Strainer (6) is covered in the large port of funnel (8)；
Suction means includes suction pipe (10), and suction pipe (10) is connect with funnel (8) portlet.
2. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that funnel
(8) large port is connected with external screw thread ring (9), and external screw thread ring (9) is that outer surface has externally threaded circular ring structure；
The end of the one end being connected on body (3) with funnel (8) is provided with the internal screw thread with external screw thread ring (9) cooperation in inner surface
(4)；
The large port of funnel (8) protrudes into body (3), and the outer of funnel (8) large port is embedded with rubber seal ring (7), funnel (8)
It is sealed by rubber seal ring (7) and body (3).
3. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that body
(3) port of one end is equipped with the limit card slot (1) for limiting to embolism (2).
4. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that embolism
(2) it is rubber embolism, or is hard embolism；When embolism (2) are hard embolism, on embolism (2) with the contact of body (3)
The sealing ring for forming sealing between body (3) is arranged on face.
5. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that suction pipe
(10) end is connected with spade suction nozzle (11).
6. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that strainer
It (6) is flexible filter screen.
7. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that strainer
(6) aperture is 1~1.5mm.
8. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that suction pipe
It (10) is flexible pipe, inner surface is coated with adhesive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822150106.6U CN209342476U (en) | 2018-12-19 | 2018-12-19 | A kind of device for the transfer of neural tissue slice multisample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822150106.6U CN209342476U (en) | 2018-12-19 | 2018-12-19 | A kind of device for the transfer of neural tissue slice multisample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209342476U true CN209342476U (en) | 2019-09-03 |
Family
ID=67758100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822150106.6U Expired - Fee Related CN209342476U (en) | 2018-12-19 | 2018-12-19 | A kind of device for the transfer of neural tissue slice multisample |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109443878A (en) * | 2018-12-19 | 2019-03-08 | 中国人民解放军第四军医大学 | A kind of device and method for the transfer of neural tissue slice multisample |
-
2018
- 2018-12-19 CN CN201822150106.6U patent/CN209342476U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109443878A (en) * | 2018-12-19 | 2019-03-08 | 中国人民解放军第四军医大学 | A kind of device and method for the transfer of neural tissue slice multisample |
| CN109443878B (en) * | 2018-12-19 | 2023-11-10 | 中国人民解放军第四军医大学 | Device and method for transferring multiple samples of nerve tissue slice |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190903 Termination date: 20201219 |