CN109443878A - A kind of device and method for the transfer of neural tissue slice multisample - Google Patents
A kind of device and method for the transfer of neural tissue slice multisample Download PDFInfo
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- CN109443878A CN109443878A CN201811557824.3A CN201811557824A CN109443878A CN 109443878 A CN109443878 A CN 109443878A CN 201811557824 A CN201811557824 A CN 201811557824A CN 109443878 A CN109443878 A CN 109443878A
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- tissue slice
- embolism
- multisample
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- 230000001537 neural effect Effects 0.000 title claims abstract description 64
- 238000012546 transfer Methods 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 19
- 208000005189 Embolism Diseases 0.000 claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 239000002699 waste material Substances 0.000 claims abstract description 17
- 238000007789 sealing Methods 0.000 claims description 8
- 239000000853 adhesive Substances 0.000 claims description 4
- 230000001070 adhesive effect Effects 0.000 claims description 4
- 230000006641 stabilisation Effects 0.000 abstract description 3
- 238000011105 stabilization Methods 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000005484 gravity Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 210000004126 nerve fiber Anatomy 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 244000287353 Crassocephalum crepidioides Species 0.000 description 1
- 240000003759 Erodium cicutarium Species 0.000 description 1
- 235000009967 Erodium cicutarium Nutrition 0.000 description 1
- 206010016322 Feeling abnormal Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to Neurobiology fields, and in particular to a kind of device and method for the transfer of neural tissue slice multisample, the device for the transfer of neural tissue slice multisample includes negative pressure device, filter device and suction means；Negative pressure device includes body and embolism, and body is the hollow tubular of both ends open；Embolism is set in body, seals between embolism and body and embolism can be slided along body；Filter device includes funnel and strainer, and the large port of funnel is removably mounted to one end of body and seals between body；Strainer is covered in the large port of funnel；Suction means includes suction pipe, and suction pipe is connect with funnel portlet.The present invention for the transfer of neural tissue slice multisample apparatus structure is compact, stabilization easy to operate, can disposably shift multisample and without waste liquid, greatly improve experimental study personnel and carry out the efficiency that neural tissue slice changes liquid, is incubated for the operations such as antibody.
Description
Technical field
The invention belongs to Neurobiology fields, and in particular to a kind of device for the transfer of neural tissue slice multisample
And method.
Background technique
Neural tissue slice is typically derived from the brain and spinal cord of rodent, is answered extensively in Neuroscience Research field
A variety of slice types such as a kind of specimen types, including frozen section, paraffin section, Gorky's slice, ultra-thin section.With
Brain or myeloid tissue of the PBS lavation after perhaps paraformaldehyde is fixed completely stripped out out of animal body steep into Gorky's liquid or
After sucrose dehydration, need to obtain the tissue specimen that 20 μm of -200 μ m thick does not wait in slicer according to experiment, from
And carry out corresponding experiment process.
Different neural tissue slice quality are different, carry out generally requiring to carry out for its histotomy property when experiment process
Select the transfer tool to avoid unnecessary tissue damage, while we are also required to carry out the transfer of multisample to improve experiment
Efficiency.Laboratory often takes following methods to carry out slice transfer at present: if shifting histotomy using metal crowfoot, first is that
The limited sample size that the time is longer and can shift is expended, second is that shifting the gravity that the more crisp Gorky of quality is sliced Shi Yinshui
It usually will cause tissue fracture；Though also bringing along corresponding amount simultaneously if can disposably shift great amount of samples using thick head straw
Waste liquid influence subsequent experimental processing；It is sliced if being shifted using filter plate, only can be carried out simple storage and changes liquid processing, it cannot
Carry out the operation such as transfer processing between various sizes of Experimental Establishment, such as antibody incubation.The above method causes entirely to shift
Process efficiency is low, time and effort consuming, cannot achieve the multisample transfer operation of the neural tissue slice of polymorphic type.Therefore, it is necessary to
Design it is a kind of can once shift a large amount of neural tissue slices, and can guarantee that transfer process does not have the device of too many waste liquid.
Summary of the invention
It is a kind of for neural tissue slice multisample in view of the deficiencies of the prior art, the present invention intends to provide
The device and method of transfer, device through the invention can disposably shift a large amount of nerve slices, and guarantee transfer process
In do not contain waste liquid.
In order to solve the above-mentioned technical problem, the present invention is realised by adopting the following technical scheme:
A kind of device for the transfer of neural tissue slice multisample, including negative pressure device, filter device and suction means；
Negative pressure device includes body and embolism, and body is the hollow tubular of both ends open；Embolism is set in body, embolism
It is sealed between body and embolism can be slided along body；
Filter device includes funnel and strainer, and the large port of funnel is removably mounted to one end of body and between body
Sealing；Strainer is covered in the large port of funnel；
Suction means includes suction pipe, and suction pipe is connect with funnel portlet.
The large port of funnel is connected with external screw thread ring, and external screw thread ring is that outer surface has externally threaded circular ring structure；Body
The end of upper one end being connected with funnel is provided with the internal screw thread with the cooperation of external screw thread ring in inner surface；
The large port of funnel protrudes into body, and the outer of funnel large port is embedded with rubber seal ring, and funnel is close by rubber
Seal ring and body seal.
The port of body one end is equipped with the limit card slot for limiting to embolism.
Embolism is rubber embolism, or is hard embolism；When embolism is hard embolism, in the contact with body on embolism
The sealing ring for forming sealing between body is arranged on face.
The end of suction pipe is connected with spade suction nozzle.
Strainer is flexible filter screen.
The aperture of strainer is 1~1.5mm.
Suction pipe is flexible pipe, and inner surface is coated with adhesive.
A method of it shifts for neural tissue slice multisample, is turned using above-mentioned for neural tissue slice multisample
The device of shifting carries out, and process is as follows:
S1: lifting embolism, by the neural tissue slice of required transfer from suction pipe suction funnel；
S2: body is flipped upside down, and makes the big end mouth down of funnel, is mixed in the waste liquid in neural tissue slice by gravity
It acts through strainer to filter out, neural tissue slice is trapped in funnel；
S3: after the waste liquid wait be mixed in neural tissue slice filters out, funnel is separated with body, then is changed into body
Funnel and body, are then installed together by clean new liquid；Alternatively, first separating funnel with body, then changed into body
Clean new liquid after the waste liquid wait be mixed in neural tissue slice filters out, then funnel and body is installed together；
S4: pushing embolism, and the clean new liquid being packed into body enters funnel from strainer, and the nerve fiber washed away in funnel is cut
Neural tissue slice simultaneously is discharged along suction pipe in piece.
The invention has the following beneficial effects:
It is sealed between device embolism and body for the transfer of neural tissue slice multisample of the invention and embolism can
It is slided along body, the large port of funnel is removably mounted to one end of body and seals between body；Strainer is covered in funnel
Large port；Suction pipe is connect with funnel portlet；When shifting neural tissue slice, lifting embolism can make to generate inside body
Negative pressure, the negative pressure of generation is by the neural tissue slice of required transfer from suction pipe suction funnel；It sucks and then overturns body
It is inverted, makes the big end mouth down of funnel, the waste liquid being mixed in neural tissue slice at this time is filtered out by gravity by strainer,
Neural tissue slice is trapped in funnel；Then: after the waste liquid wait be mixed in neural tissue slice filters out, by funnel and body
Separation, then clean new liquid is changed into body, then funnel and body are installed together；Alternatively, first by funnel and body
Separation, then changes to clean new liquid into body, after the waste liquid wait be mixed in neural tissue slice filters out, then by funnel and bottle
Body is installed together；Embolism is finally pushed, the clean new liquid being packed into body enters funnel from strainer, washes away the nerve in funnel
Neural tissue slice simultaneously is discharged along suction pipe in histotomy.The present invention is used for the apparatus structure of neural tissue slice multisample transfer
Stabilization compact, easy to operate can disposably shift multisample and be free of waste liquid, greatly improve experimental study personnel and carry out mind
The efficiency changed liquid through histotomy, be incubated for the operations such as antibody.
The beneficial effect of the device of neural tissue slice multisample transfer is used for it is found that the present invention is used for by aforementioned present invention
The method of neural tissue slice multisample transfer operates stabilization easy to operate, and can disposably shift multisample and be free of useless
Liquid greatly improves experimental study personnel and carries out the efficiency that neural tissue slice changes liquid, is incubated for the operations such as antibody.
Detailed description of the invention
Fig. 1 is the overall structure diagram that the present invention is used for neural tissue slice multisample transfer device；
Fig. 2 is the structural schematic diagram of negative pressure assembling device of the present invention；
Fig. 3 is the structural schematic diagram of filter device of the present invention and external screw thread ring；
Fig. 4 is the structural schematic diagram of suction tube device of the present invention.
In figure, 1- limit card slot, 2- embolism, 3- body, 4- internal screw thread, 6- strainer, 7- rubber seal ring, 8- funnel, 9-
External screw thread ring, 10- suction pipe, 11- spade suction nozzle.
Specific embodiment
It is next with reference to the accompanying drawings and examples that the present invention is further illustrated.
Referring to Fig.1, the device for the transfer of neural tissue slice multisample of the invention, including negative pressure device, filtering dress
It sets and suction means；Wherein, negative pressure device includes body 3 and embolism 2, and body 3 is the hollow tubular of both ends open；Embolism 2 is set
It is placed in body 3, is sealed between embolism 2 and body 3 and embolism 2 can be slided along body 3；Filter device includes funnel 8 and filter
Net 6, the large port of funnel 8 are removably mounted to one end of body 3 and seal between body 3；Strainer 6 is covered in funnel 8
Large port；Suction means includes suction pipe 10, and suction pipe 10 is connect with 8 portlet of funnel.
As shown in figure 3, referring to Figures 1 and 2, as the preferred embodiment of the invention, the large port of funnel 8 is connected with outer
Screwed ring 9, external screw thread ring 9 are that outer surface has externally threaded circular ring structure；The end of the one end being connected on body 3 with funnel 8
The internal screw thread 4 cooperated with external screw thread ring 9 is provided in inner surface；The large port of funnel 8 protrudes into body 3,8 large port of funnel
Outer is embedded with rubber seal ring 7, and funnel 8 is sealed by rubber seal ring 7 and body 3.When in use, by by external screw thread ring 9
On 3 lower end of external screw thread and body internal screw thread between mating connection so that funnel 8 realize and body 3 between it is detachable
Connection, while can be fitted closely between rubber seal ring 7 and the inner wall of body 3, it realizes close between funnel 8 and body 3
Envelope, therefore negative pressure can be generated in the inner cavity of body 3 when lifting embolism 2 upwards, the negative pressure of generation can be by required transfer
Neural tissue slice is from 10 suction funnel 8 of suction pipe.
As depicted in figs. 1 and 2, as the preferred embodiment of the invention, the port of 3 upper end of body is equipped with for embolism
The limit card slot 1 of 2 limits, limit card slot 1 can prevent embolism 2 from deviating from during lifting from 3 upper port of body.
As the preferred embodiment of the invention, embolism 2 is rubber embolism, or is hard embolism；When embolism 2 is hard
The sealing ring for forming sealing between body 3 is arranged with when embolism, on embolism 2 on the contact surface of body 3, passes through this
The sealed connection of two kinds of forms, the sealing and embolism 2 that can be realized between embolism 2 and body 3 can be slided along body 3.
As the preferred embodiment of the invention, the end of suction pipe 10 is connected with spade suction nozzle 11, convenient for drawing in corner
Sample.
As the preferred embodiment of the invention, strainer 6 is that flexible filter screen can be sent out since strainer is flexible
Change shape, and during pushing embolism 2 that neural tissue slice is discharged along suction pipe 10, the deformation of flexible filter screen is readily able to make
It is separated between neural tissue slice and strainer 6.
As the preferred embodiment of the invention, the aperture of strainer 6 is 1~1.5mm.
As the preferred embodiment of the invention, suction pipe 10 is flexible pipe, and inner surface is coated with adhesive, adhesive energy
Enough prevent that neural tissue slice is Nian Jie with suction pipe 10 inner wall generation and causes to damage.
The process that neural tissue slice multisample is shifted using the device that neural tissue slice multisample of the present invention shifts
It is as follows:
S1: lifting embolism 2, by the neural tissue slice of required transfer from 10 suction funnel 8 of suction pipe；
S2: body 3 is flipped upside down, and makes the big end mouth down of funnel 8, is mixed in the waste liquid in neural tissue slice by weight
Power acts through strainer 6 and filters out, and neural tissue slice is trapped in funnel 8；
S3: after the waste liquid wait be mixed in neural tissue slice filters out, funnel 8 is separated with body 3, then into body 3
Funnel 8 and body 3, are then installed together by the clean new liquid of change；Alternatively, first funnel 8 is separated with body 3, then to bottle
Clean new liquid is changed in body 3, after the waste liquid wait be mixed in neural tissue slice filters out, then funnel 8 and body 3 is mounted on
Together；
Embolism 2 is pushed, the clean new liquid being packed into body 3 enters funnel 8 from strainer 6, washes away the nerve fiber in funnel 8
It is sliced and neural tissue slice is discharged along suction pipe 10.
Claims (9)
1. it is a kind of for neural tissue slice multisample transfer device, which is characterized in that including negative pressure device, filter device and
Suction means；
Negative pressure device includes body (3) and embolism (2), and body (3) is the hollow tubular of both ends open；Embolism (2) is set to bottle
In body (3), is sealed between embolism (2) and body (3) and embolism (2) can be slided along body (3)；
Filter device includes funnel (8) and strainer (6), the large port of funnel (8) be removably mounted to one end of body (3) and with
Body seals between (3)；Strainer (6) is covered in the large port of funnel (8)；
Suction means includes suction pipe (10), and suction pipe (10) is connect with funnel (8) portlet.
2. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that funnel
(8) large port is connected with external screw thread ring (9), and external screw thread ring (9) is that outer surface has externally threaded circular ring structure；
The end of the one end being connected on body (3) with funnel (8) is provided with the internal screw thread with external screw thread ring (9) cooperation in inner surface
(4)；
The large port of funnel (8) protrudes into body (3), and the outer of funnel (8) large port is embedded with rubber seal ring (7), funnel (8)
It is sealed by rubber seal ring (7) and body (3).
3. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that body
(3) port of one end is equipped with the limit card slot (1) for limiting to embolism (2).
4. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that embolism
(2) it is rubber embolism, or is hard embolism；When embolism (2) are hard embolism, in the contact surface with body 3 on embolism (2)
On be arranged with sealing ring for forming sealing between body (3).
5. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that suction pipe
(10) end is connected with spade suction nozzle (11).
6. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that strainer
It (6) is flexible filter screen.
7. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that strainer
(6) aperture is 1~1.5mm.
8. a kind of device for the transfer of neural tissue slice multisample according to claim 1, which is characterized in that suction pipe
It (10) is flexible pipe, inner surface is coated with adhesive.
9. a kind of method for the transfer of neural tissue slice multisample, which is characterized in that use claim 1-8 any one
The device for the transfer of neural tissue slice multisample carries out, and process is as follows:
S1: lifting embolism (2), by the neural tissue slice of required transfer from suction pipe (10) suction funnel (8)；
S2: body (3) is flipped upside down, and makes the big end mouth down of funnel (8), is mixed in the waste liquid in neural tissue slice by weight
Power acts through strainer (6) and filters out, and neural tissue slice is trapped in funnel (8)；
S3: after the waste liquid wait be mixed in neural tissue slice filters out, funnel (8) being separated with body (3), then to body (3)
Funnel (8) and body (3), are then installed together by the clean new liquid of middle change；Alternatively, first by funnel (8) and body (3) point
From, then the new liquid clean to change in body (3), after the waste liquid wait be mixed in neural tissue slice filters out, then by funnel (8)
It is installed together with body (3)；
S4: pushing embolism (2), and the clean new liquid being packed into body (3) enters funnel (8) from strainer (6), washes away in funnel (8)
Neural tissue slice and make neural tissue slice along suction pipe (10) be discharged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811557824.3A CN109443878B (en) | 2018-12-19 | 2018-12-19 | Device and method for transferring multiple samples of nerve tissue slice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811557824.3A CN109443878B (en) | 2018-12-19 | 2018-12-19 | Device and method for transferring multiple samples of nerve tissue slice |
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| Publication Number | Publication Date |
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| CN109443878A true CN109443878A (en) | 2019-03-08 |
| CN109443878B CN109443878B (en) | 2023-11-10 |
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| CN201811557824.3A Active CN109443878B (en) | 2018-12-19 | 2018-12-19 | Device and method for transferring multiple samples of nerve tissue slice |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113040840A (en) * | 2021-02-23 | 2021-06-29 | 长沙众智医疗器械有限责任公司 | Nerve guiding device |
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| CN109443878B (en) | 2023-11-10 |
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