CN206311612U - A kind of test device of Daphnia magna acute toxicity - Google Patents
A kind of test device of Daphnia magna acute toxicity Download PDFInfo
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- CN206311612U CN206311612U CN201621384437.0U CN201621384437U CN206311612U CN 206311612 U CN206311612 U CN 206311612U CN 201621384437 U CN201621384437 U CN 201621384437U CN 206311612 U CN206311612 U CN 206311612U
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- China
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- test device
- daphnia magna
- magna
- acute toxicity
- test
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- Farming Of Fish And Shellfish (AREA)
Abstract
The utility model discloses a kind of test device of Daphnia magna acute toxicity, described device includes that Daphnia magna cultivates separator, storage Magna devices, test device, shell, wireless launcher, the Portable toxicity test device carries out toxic reaction with the prepare liquid that Daphnia magna and various concentrations are matched by cultivating and separating test in test device, the wireless launcher collection image, temperature, pH relevant informations are transferred to computer end carries out operational analysis, can also realize remotely supervising function.
Description
Technical field
The utility model belongs to field of environment protection, and in particular to a kind of test device of Daphnia magna acute toxicity.
Background technology
In the serious today's society of water pollution, either lake river or city domestic water all suffer from the shape of sternness
Gesture, so the method that not only seek purified water, will also carry out the monitoring and detection of water resource, realizes that preventing and treating is combined, and toxicity is surveyed
Examination is detected as common with chemical reagent, but chemical reagent influenceed by some water quality and toxicity range unavoidably, and there is secondary dirt
Dye problem, is especially unsuitable for the detection of city domestic sewage, and in recent years, animal toxicity detection has very well in water pollution context of detection
Development, toxicity test be to experimental animal carry out different approaches, different maturity periods contamination, detect the reality of various toxic endpoints
Test, be safety evaluatio or danger the purpose is to determine harmless exposure level, pathotype, target organ, dose-response relationship
Property evaluation important data is provided.Acute toxicity test refers to repeatedly to animal contaminated once or in 24 hours, for detecting
The lethal dose (concentration) of chemicals and cause the minimum dose (concentration) of detrimental effect.Daphnia magna lives in natural water area, category
Flotation crustaceans animal, short with growth cycle, breeding is fast, economic easily culture, is international public the features such as high to poisonous substance sensitiveness
The standard toxicity test recognized is biological.The biological test technology of Daphnia magna has had wide research abroad, but China exists
The research of this respect also has a certain distance therewith, how to realize that Daphnia magna is quick in China's water resource detection, easy, fits
The research purpose extensively waited with scope, is exactly the problem to be studied of the utility model.
Utility model content
The purpose of this utility model is to provide a kind of test device of Daphnia magna acute toxicity.
To achieve the above object, a kind of test device of Daphnia magna acute toxicity, described device includes that Daphnia magna is cultivated and divides
From device, storage Magna devices, test device, shell, wireless launcher, the Daphnia magna cultivates separator includes temperature adjustment control
Device processed one, seperation film, chlorination equipment, bait thrower, culturing room, pH-meter, temperature control layer, separation chamber, the storage Magna devices lead to
Piping is cultivated separator and is connected with Daphnia magna, and its built-in sample storage room, camera one, the test device include camera
2nd, charger, flow control valve, fluorescent lamp, air-breather, camera three, temperature adjustment control device two, Water Tank with Temp.-controlled, survey
Trial jar；The camera is high-definition camera；The Portable toxicity test device is by culture and separates test
The prepare liquid matched with Daphnia magna and various concentrations carries out toxic reaction in test device, and the wireless launcher gathers shadow
Picture, temperature, pH relevant informations are transferred to computer end carries out operational analysis.
Further, the chlorination equipment of stating uses disinfection by ultraviolet light, and simple and convenient, Disinfection Effect is good, and not having
Material residual is learned so as to influence the cultivation of Daphnia magna.
Further, the separation membrane aperture is 2.0mm.
Further, bait includes algae, organic fertilizer, yeast in the bait thrower, and bait throwing in is convenient, and daily ration, feeding quantity is easy to control
System.
Further, what the air-breather was passed through is filtrated air, it is to avoid extraneous miscellaneous bacteria is to Daphnia magna toxic reaction
Interference.
Further, corresponding label is respectively marked with the charger and test bottle, can as needed from many during test
Individual not isoconcentration poisonous substance gradient.
Further, the Daphnia magna for being unsuitable for toxicity test that the seperation film is eliminated out can centralized recovery as fish raise
Material.
Further, the Daphnia magna can be used in Daphnia magna cultivating process in cultivating separator according to reality
Fluorescent lamp is installed in sunlight additional, and irradiation time is 8-10h.
The utility model has the advantage of：A kind of test device realization culture of Daphnia magna acute toxicity of the utility model,
Store, test in one, it is easy to operate, monitor in real time is carried out by three cameras, and combining wireless emitter is realized far
Journey is manipulated, and the scope of application is wider, the design of multigroup test bottle, and the toxicity prepare liquid for being capable of achieving the various concentration gradients of proportioning is seen
Examine, improve the degree of accuracy of test.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the test device of Daphnia magna acute toxicity；
Fig. 2 is a kind of test flow chart of the test device of Daphnia magna acute toxicity；
Wherein, 1- Daphnia magnas cultivate separator；2- stores up Magna devices；3- test devices；4- shells；5- temperature adjustments
Control device one；6- seperation films；7- chlorination equipments；8- bait throwers；9- culturing room；10-pH adjusters；11- temperature controls layer；12- points
From room；13- pipelines；14- sample storages room；15- cameras one；16- cameras two；17- chargers；18- wireless launchers；19- flows
Adjustable valve；20- fluorescent lamps；21- air-breathers；22- cameras three；23- temperature adjustments control device two；24- Water Tanks with Temp.-controlled；
25- test bottles.
Specific embodiment
Hereinafter, with reference to accompanying drawing 1-2 and embodiment, specific embodiment of the present utility model is described in detail：
A kind of test device of Daphnia magna acute toxicity of the present utility model is shown such as Fig. 1, described device includes Daphnia magna
Separator 1, storage Magna devices 2, test device 3, shell 4, wireless launcher 18 are cultivated, the Daphnia magna cultivates separator 1
Including temperature adjustment control device 1, seperation film 6, chlorination equipment 7, bait thrower 8, culturing room 9, pH-meter 10, temperature control layer
11st, separation chamber 12, the storage Magna devices 2 are cultivated separator 1 and are connected by pipeline 13 and Daphnia magna, its built-in sample storage room 14,
Camera 1, the test device 3 includes camera 2 16, charger 17, flow control valve 19, fluorescent lamp 20, ventilation dress
Put 21, camera 3 22, temperature adjustment control device 2 23, Water Tank with Temp.-controlled 24, test bottle 25；The Portable toxicity test dress
Put carries out toxicity with the prepare liquid that Daphnia magna and various concentrations are matched by cultivating and separating test in test device 3
Reaction, the collection of the wireless launcher 18 image, temperature, pH relevant informations are transferred to computer end carries out operational analysis；It is described to state
Chlorination equipment 7 uses disinfection by ultraviolet light, simple and convenient, and Disinfection Effect is good, and it is large-scale so as to influence not have chemical residue
The cultivation of Magna；The aperture of the seperation film 6 is 2.0mm；Bait includes algae, organic fertilizer, yeast, bait throwing in the bait thrower 8
Convenient, daily ration, feeding quantity is easy to control；What the air-breather 21 was passed through is filtrated air, it is to avoid extraneous miscellaneous bacteria is anti-to Daphnia magna toxicity
The interference answered；Corresponding label is respectively marked with the charger 17 and test bottle 25, multiple can be selected not as needed during test
Isoconcentration poisonous substance gradient；The Daphnia magna of what the seperation film 6 was eliminated out be unsuitable for toxicity test can centralized recovery raised as fish
Material；Can be according to reality is using sunlight or adds in Daphnia magna cultivating process in the Daphnia magna cultivation separator
Dress fluorescent lamp, irradiation time is 8-10h.
A kind of method of testing of the test device of Daphnia magna acute toxicity is：
A. the cultivation and separation of Daphnia magna, 20-24 DEG C of temperature, pH7.5-8.5 are carried out in Daphnia magna cultivates separator 1
Under the conditions of, breeding is turned out soon by bait throwing in, vitality is vigorous, well-developed Daphnia magna, then isolated by seperation film 6
Magna is used in experiment；
B. isolated experiment is incorporated into storage Magna devices 2 with Magna and is stored, and is easy to experiment with taking；
C. during toxotest, the toxicity prepare liquid for configuring various concentrations gradient is flowed into equipped with 3-5 examination by charger 17
Test in the test bottle 2 with Magna, wherein one group of blank control group to be measured of thing toxicity is must be provided with, it is anti-under 18-22 DEG C of water bath with thermostatic control
Should, the activity change and mortality of observation Magna, its half effect concentration EC50 is calculated by range estimation or computer system in 24h.
The utility model is not limited to above-mentioned implementation method, in the case of without departing substantially from substance of the present utility model,
Art processes personnel it is conceivable that any deformation, improvement, replacement each fall within scope of the present utility model.
Claims (6)
1. a kind of test device of Daphnia magna acute toxicity, it is characterised in that described device includes that Daphnia magna cultivates separator
(1), storage Magna devices (2), test device (3), shell (4), wireless launcher (18), the Daphnia magna cultivate separator (1)
Including temperature adjustment control device one (5), seperation film (6), chlorination equipment (7), bait thrower (8), culturing room (9), pH-meter
(10), temperature control layer (11), separation chamber (12), storage Magna devices (2) cultivate separator (1) by pipeline (13) and Daphnia magna
It is connected, its built-in sample storage room (14), camera one (15), the test device (3) include camera two (16), charger
(17), flow control valve (19), fluorescent lamp (20), air-breather (21), camera three (22), temperature adjustment control device two
(23), Water Tank with Temp.-controlled (24), test bottle (25).
2. a kind of test device of Daphnia magna acute toxicity as claimed in claim 1, it is characterised in that described chlorination equipment
(7) disinfection by ultraviolet light is used.
3. a kind of test device of Daphnia magna acute toxicity as claimed in claim 1, it is characterised in that the seperation film (6)
Aperture is 2.0mm.
4. a kind of test device of Daphnia magna acute toxicity as claimed in claim 1, it is characterised in that the bait thrower (8)
Middle bait includes algae, organic fertilizer, yeast.
5. a kind of test device of Daphnia magna acute toxicity as claimed in claim 1, it is characterised in that the air-breather
(21) what is be passed through is filtrated air.
6. a kind of test device of Daphnia magna acute toxicity as claimed in claim 1, it is characterised in that the charger (17)
Corresponding label is respectively marked with test bottle (25).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621384437.0U CN206311612U (en) | 2016-12-16 | 2016-12-16 | A kind of test device of Daphnia magna acute toxicity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621384437.0U CN206311612U (en) | 2016-12-16 | 2016-12-16 | A kind of test device of Daphnia magna acute toxicity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206311612U true CN206311612U (en) | 2017-07-07 |
Family
ID=59251782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621384437.0U Expired - Fee Related CN206311612U (en) | 2016-12-16 | 2016-12-16 | A kind of test device of Daphnia magna acute toxicity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206311612U (en) |
-
2016
- 2016-12-16 CN CN201621384437.0U patent/CN206311612U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170707 Termination date: 20171216 |