CN107102003A - A kind of bloom blue algae simulated experiment monitoring system and method - Google Patents
A kind of bloom blue algae simulated experiment monitoring system and method Download PDFInfo
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- CN107102003A CN107102003A CN201710536750.4A CN201710536750A CN107102003A CN 107102003 A CN107102003 A CN 107102003A CN 201710536750 A CN201710536750 A CN 201710536750A CN 107102003 A CN107102003 A CN 107102003A
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Classifications
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- G—PHYSICS
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G06F—ELECTRIC DIGITAL DATA PROCESSING
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
Abstract
The invention provides a kind of bloom blue algae simulated experiment monitoring system, including:Culture apparatus, for holding the bed mud containing rest period blue-green algae and sample point water body;Multiple imaging devices, are uniformly arranged in outside the side wall of culture apparatus layer by layer, are located at growth image of the blue-green algae in different water layers in different time points in culture apparatus for shooting;Remote monitoring device, is connected with imaging device, is located at characteristic parameter of the blue-green algae floccule body in different time points of different water layers during algal bloom for receiving and handling the image of imaging device transmission and obtaining.Present invention also offers a kind of bloom blue algae simulated experiment monitoring method.The bloom blue algae simulated experiment monitoring system that the present invention is provided, the outburst information of bloom blue algae can be grasped quickly, comprehensively, understood it and broken out mechanism.
Description
Technical field
The present invention relates to water plant simulation growth field, more particularly to a kind of bloom blue algae simulated experiment monitoring system and
Method.
Background technology
At present, algal bloom is one of worldwide water environmental problems the most prominent, and the development with global economy and
The expansion of the effect of human activity and be on the rise.China's bloom blue algae phenomenon is particularly acute, most of rivers, lake even reservoir
In all have the generation of wawter bloom to some extent, the lake algal bloom such as Taihu Lake, Dian Chi, Chaohu is even more to occur now and then.Bloom blue algae
Phenomenon large area, take place frequently for a long time, not only broken up function of lake and ecological environment, and threaten health and life drink
With the safety of water, oneself turns into one of main Environmental Problems of puzzlement China's economic sustainable development.But at present, people are blue to wawter bloom
The formation mechenism of algae is not completely clear and definite, also in the further investigation stage.
Why blue-green algae forms the sociales of wawter bloom, is that they all have a kind of structure-puppet for adjusting cell settlement empty
Born of the same parents, by the air bag number and volume in the pseudo- ghost of dynamic regulation, enable blue-green algae to control its buoyancy.Based on its unique physiology
(for example there is feature pseudo- vacuole to cause it has to float for the formation of cell colony, cell caused by the exocellular polysaccharide of cell secretion
Physiology to the water surface tends to), under a series of conditionings such as light intensity, temperature, wave disturbance, blue-green algae forms flocculation by colliding
Big colony, and the floating upward quickly formation visible wawter bloom in surface --- i.e. wawter bloom " outburst ".It is generally acknowledged that bloom blue algae formation includes phase
Mutually difference and continuously Four processes:Sinking and survive the winter (dormancy), --- floating shape is assembled in recovery --- biomass increase ---
Into wawter bloom, and understand blue-green algae recovery --- onset process bloom prealarming with control in it is significant.
Because the resuscitation process of bloom blue algae is considerably complicated, simultaneously as the limitation of field monitoring technology, blue for wawter bloom
Algae recovers and the understanding of process still neither one completely from bed mud into water body to outburst floating.Therefore, in a short time
In the case that bloom blue algae problem still seldom arrives basic solution, for quick, comprehensive grasp bloom blue algae outburst information, simulation is ground
Study carefully the excrescent method and apparatus of blue-green algae to be particularly important.
The content of the invention
For problem above, it can be floated it is an object of the invention to provide one kind in experiment lab simulation bloom blue algae recovery
The experimental simulation monitoring system of process, is monitored by three layers of the micro- digital imaging devices point upper, middle and lower of high-resolution and shoots wawter bloom
Blue-green algae recovers-formed the process of wawter bloom, calculates the method that algal bloom process related parameters are demonstrated with three-dimensional simulation.Pass through the palm
Hold blue-green algae to recover under water the process and feature of growth, the three-dimensional demonstration figure of simulation algal bloom is made, to further appreciate that water
Experimental basis is provided from the basic law that bed mud is migrated into water in the Chinese blue algae recovery cycle, blue-green algae in wawter bloom forming process is understood
Colony migrates and specific hierarchical and distributed mechanism in water body, is provided accurately further to formulate forecast and blue algae prevention outburst
Data and technical support.
A kind of bloom blue algae simulated experiment monitoring system, including:
Culture apparatus, for holding the bed mud containing rest period blue-green algae and sample point water body;
Multiple imaging devices, are uniformly arranged in outside the side wall of the culture apparatus layer by layer, for shooting the culture dress
It is located at growth image of the blue-green algae in different water layers in different time points in putting;
Remote monitoring device, is connected with the imaging device, the image for receiving and handling the imaging device transmission
And obtain characteristic parameter of the blue-green algae floccule body in different time points for being located at different water layers during algal bloom.
In above-mentioned bloom blue algae simulated experiment monitoring system, a kind of preferred embodiment, the blue-green algae floccule body are used as
Characteristic parameter be blue-green algae floccule body projected area A, maximum perimeter P and maximum length L.
In above-mentioned bloom blue algae simulated experiment monitoring system, as a kind of preferred embodiment, outside the culture apparatus
Portion is arranged with outer layer clamping device, space is left between the side wall of the culture apparatus and the outer layer clamping device, for water
Flow to regulate and control the water temperature in the culture apparatus;Preferably, the side of the culture apparatus and the outer layer clamping device
Distance between wall is 10~15cm (such as 10.5mm, 11mm, 12mm, 13mm, 14mm, 14.5mm).
In above-mentioned bloom blue algae simulated experiment monitoring system, as a kind of preferred embodiment, the system also includes
Water feeder is circulated, the space between the culture apparatus and the outer layer clamping device side wall connects, with to the space
Middle water of the supply with specified temp, it is highly preferred that outlet and the outer layer clamping device of the circulation water feeder
Entrance is connected, and the outlet of the outer layer clamping device is connected with the entrance of the circulation water feeder.
In above-mentioned bloom blue algae simulated experiment monitoring system, a kind of preferred embodiment, the recirculated water supply are used as
Device includes:Tank;Thermal Control Element, is arranged on the tank;Temp probe, is arranged in the tank, for detecting
State the water temperature in tank;Temperature controller, is arranged on outside the tank, connects respectively with the temp probe and the Thermal Control Element
Connect, control the Thermal Control Element to adjust the water temperature in the tank according to the temp probe feedack;Preferably, it is described
The leaving water temperature of tank is 10~40 DEG C (such as 11 DEG C, 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 39 DEG C).
In above-mentioned bloom blue algae simulated experiment monitoring system, as a kind of preferred embodiment, the culture apparatus is
The square cylinder of top surface opening, the culture apparatus is made up of transparent organic glass;Preferably, the wall thickness of the culture apparatus is
7-9mm (such as 7.5mm, 8mm, 8.5mm).
In above-mentioned bloom blue algae simulated experiment monitoring system, a kind of preferred embodiment, the outer layer chuck dress are used as
The square cylinder of top surface opening is set to, the outer layer clamping device is made up of transparent PE material or glass;Preferably, the outer layer
The wall thickness of clamping device is 7-9mm (such as 7.5mm, 8mm, 8.5mm).
It is highly preferred that black pigment is coated with four perisporiums of the outer layer clamping device and the outer surface of bottom wall, so that
Four perisporiums and bottom wall of the culture apparatus are closed light tight.
In above-mentioned bloom blue algae simulated experiment monitoring system, as a kind of preferred embodiment, in the outer layer chuck
Multiple through holes are set on four the week side of boss walls of device, and through hole penetrates outer layer clamping device and extended to along the inwall of outer layer clamping device
The culture apparatus outside wall surface;Imaging device is arranged in through hole;The size of the through hole matches with the imaging device, institute
The quantity for stating imaging device is identical with the quantity of the through hole;It is highly preferred that the imaging device be arranged on by support it is described
In through hole.
Preferably, 3 layers are set on the side wall of the outer layer clamping device mutually along the circumferential of the outer layer clamping device
Parallel through hole, every layer is uniformly arranged in 12 through holes, 3 layers of through hole, and first layer through hole is disposed in proximity to the culture dress
The bottom put, so that the imaging device shoots the image in blue-green algae recovery period;Second layer through hole is arranged at the culture apparatus
The middle part of middle water body, so that the imaging device photographs the image in blue-green algae floating period;Third layer through hole is arranged at the training
The top of water body in device is supported, so that the imaging device photographs the image that blue-green algae is gathered in water surface formation wawter bloom.
In above-mentioned bloom blue algae simulated experiment monitoring system, as a kind of preferred embodiment, the imaging device is
The micro- digital imaging devices of high-resolution;The micro- digital imaging devices of high-resolution are preferably 5,000,000 pixel color CMOS
Progressive scanning picture sensor;Preferably, the remote monitoring device is computer processor;It is provided with the computer processor
Micro- digital analytical measurement system;The micro- digital analytical measurement system is preferably bright U.S. micro- digital imaging system
V9.5.2;It is highly preferred that being additionally provided with the computer processor in image mosaic, imaging importing and three-dimensional animation making software
At least one.
In above-mentioned bloom blue algae simulated experiment monitoring system, as a kind of preferred embodiment, the system also includes:
Intensity of illumination control device, is arranged on directly over the culture apparatus, for providing illumination to the culture apparatus;Preferably,
The intensity of illumination control device is formed in parallel by multiple fluorescent lamps, and each fluorescent lamp sets switch, and one is opened
One fluorescent lamp of control is closed, by controlling the unlatching quantity of the fluorescent lamp to control intensity of illumination;
Preferably, the intensity of illumination of the intensity of illumination control device be 0~10000lux (such as 100lux, 500lux,
1000lux、2000lux、3000lux、4000lux、5000lux、6000lux、7000lux、8000lux、9000lux、
9500lux、9900lux)。
In above-mentioned bloom blue algae simulated experiment monitoring system, as a kind of preferred embodiment, the system also includes supplying
Wind devices, the ventilation apparatus include air blower and air speed measuring apparatus;The air blower is arranged on above the culture apparatus, is used
In providing certain wind to culture apparatus;Preferably, the air blower is movable equipment, freely to adjust the air blower
Highly the wind speed and direction of air blower is controlled with angle;The air speed measuring apparatus is set close to the water surface of the culture apparatus
Put, the wind speed of the water surface for measuring the culture apparatus;Preferably, the wind speed of the water surface of the culture apparatus is
0~3.5m/s (such as 0.1m/s, 0.5m/s, 1.0m/s, 1.5m/s, 2.0m/s, 2.5m/s, 3.0m/s, 3.4m/s).
In above-mentioned bloom blue algae simulated experiment monitoring system, as a kind of preferred embodiment, in the culture apparatus
The N/P ratio of water body is 1~40:1 (such as 2:1、5:1、10:1、15:1、20:1、25:1、30:1、35:1、39:1).
A kind of bloom blue algae simulated experiment monitoring method, includes successively:
Step one, life of the blue-green algae in different time points for being located at different water layers in culture apparatus is gathered using imaging device
Long image, and it is sent to remote monitoring device;
Step 2, is pre-processed using the first processing system in the remote monitoring device to the growth image,
So that the growth image is apparent;
Step 3, using the second processing system in the remote monitoring device in the pretreated growth image
Blue-green algae floccule body carry out analysis measurement, obtain the characteristic parameter of the blue-green algae floccule body in each water layer of different time points, it is described
Characteristic parameter is projected area A, maximum perimeter P and the maximum length L of blue-green algae floccule body;
Step 4, the characteristic parameter of the blue-green algae floccule body obtained according to step 3 calculates algal bloom according to budget law
During be located at same water layer same image acquisition time point blue-green algae floccule body one dimensional fractal dimension D1, two-dimensional fractal
Dimension D2 and three-dimensional fractal dimension D3;
The budget law is specific as follows:
First, positioned at same water layer same image acquisition time point the blue-green algae floccule body projected area A, most
One-dimensional minute of the blue-green algae floccule body of the image acquisition time points of the big girth P and maximum length L with being located at the water layer
There is the relation as shown in formula (1)~(3) in shape dimension D1, two-dimensional fractal dimension D2 and three-dimensional fractal dimension D3:
P∝LD1(1);
A∝LD2Or A ∝ PD2(2);
V∝LD3Or V ∝ PD3(3);
Wherein, V represent the blue-green algae floccule body into sphere volume, converted by the projected area A of the blue-green algae floccule body
Arrive, circular is as follows:Tried to achieve by the projected area A of the blue-green algae floccule body straight with the circle of projected area A homalographics
Footpath dp (i.e. equivalent diameter), then the blue-green algae floccule body is calculated into sphere volume V by diameter dp;
Then, formula (1), (2), (3) both sides are taken into natural logrithm, obtains the relational expression as described in formula (4)~(6):
Ln P=D1ln L+a (4);
Ln A=D2ln P+b=D2ln L+b (5);
Ln V=D3ln P+c=D3ln L+c (6);
Afterwards, with the corresponding ln of each described blue-green algae floccule body of the same image acquisition time point positioned at same water layer
P makees ln P-ln L linear relation figures with ln L values, wherein, the slope of straight line is the image acquisition time point of the water layer
The one dimensional fractal dimension D1 of the blue-green algae floccule body, intercept is constant a;
With the corresponding ln A of each described blue-green algae floccule body of the same image acquisition time point positioned at same water layer with
Ln P or ln A make ln A-ln P or ln A-ln L linear relation figures with ln L values, wherein, the slope of straight line is the water layer
The image acquisition time point the blue-green algae floccule body two-dimensional fractal dimension D2, intercept be constant b;
With the corresponding Ln V of each described blue-green algae floccule body of the same image acquisition time point positioned at same water layer with
Ln P or Ln V make Ln V-ln P or Ln V-ln L linear relation figures with ln L values, wherein, the slope of straight line is the water layer
The image acquisition time point the blue-green algae floccule body three-dimensional fractal dimension D3, intercept be constant c.
In above-mentioned bloom blue algae simulated experiment monitoring method, moved as a kind of preferred embodiment, in addition to three-dimensional demonstration
Making step is drawn, first, the image positioned at same water layer that same image acquisition time point is gathered is carried out at splicing superposition
Reason, so as to obtain the splicing stacking chart of each image acquisition time point of each water layer;Then, according to image acquisition time
The splicing stacking chart of each water layer is fabricated in different time dimension and simulates blue-green alga bloom onset process by sequencing
Three-dimensional demonstration animation.
In above-mentioned bloom blue algae simulated experiment monitoring method, a kind of preferred embodiment, first processing system are used as
Unite as micro- digital analytical measurement system;The micro- digital analytical measurement system is preferably bright U.S. micro- digital imaging system
V9.5.2。
In above-mentioned bloom blue algae simulated experiment monitoring method, as a kind of preferred embodiment, in the step 2,
The pretreatment is carries out except making an uproar successively, correction process.
In above-mentioned bloom blue algae simulated experiment monitoring method, a kind of preferred embodiment, the second processing system are used as
Unite as image measurement and analysis software, the characteristic parameter for measuring and analyzing blue-green algae floccule body.Preferably, the second processing
System is bright U.S. micro- digital imaging system V9.5.2.
In above-mentioned bloom blue algae simulated experiment detection method, a kind of preferred embodiment, the different time points are used as
Collection blue algae growth image refers to:Start the release aggregation from bed mud in blue-green algae and float preceding every a 1-2h blue algae growth of shooting
Image, a blue algae growth image was shot when blue-green algae starts release, aggregation floating from bed mud every 5-10 minutes.
Compared with prior art, technique effect of the invention is as follows:
1st, the bloom blue algae simulated experiment monitoring system that the present invention is provided, the quick-fried of bloom blue algae can be grasped quickly, comprehensively
Photos and sending messages, understand it and break out mechanism.
2nd, recovered under water the process and feature of growth by grasping blue-green algae, the three-dimensional animation for making simulation algal bloom is drilled
Diagram, experimental basis is provided to further appreciate that in the bloom blue algae recovery cycle from the basic law that bed mud is migrated into water,
The hierarchical and distributed mechanism that blue-green algae colony migrates and assembled in water body in solution wawter bloom forming process, is that further formulate is forecast and pre-
Anti- blue algae bloom provides accurate data and technical support.
Brief description of the drawings
Fig. 1 is the schematic diagram of the bloom blue algae simulated experiment monitoring system of the embodiment of the present invention 1;
Fig. 2 is the flow chart of bloom blue algae simulated experiment monitoring method;
Reference is as follows:1-internal layer aquarium (i.e. culture apparatus), 2-outer layer chuck cylinder (i.e. outer layer clamping device),
The entrance of 21-outer layer chuck cylinder, the outlet of 22-outer layer chuck cylinder, 23- through holes, 3-water inlet pipe, 4-outlet pipe, 5-water
Groove, the entrance of 51-tank, the outlet of 52-tank, 53-Thermal Control Element, 6-temp probe, 7-temperature controller, 8-day
Light lamp, 9-air blower, 10-imaging device, 11-remote monitoring device, 12-circulation water feeder.
Embodiment
A kind of bloom blue algae simulated experiment monitoring device to the present invention is illustrated with reference to the accompanying drawings and examples.Should
Understand, these embodiments are only used for explaining the present invention rather than limitation the scope of the present invention.Externally it should be understood that reading this
After the content of invention, those skilled in the art make various changes or modifications to the present invention, and these equivalent form of values equally fall within this
Invention appended claims limited range.
The invention provides a kind of bloom blue algae simulated experiment monitoring system, including culture apparatus 1, imaging device 10 and remote
All parts and annexation in present system are illustrated, referring to Fig. 1 by journey monitoring device 11 etc. one by one below.
A kind of bloom blue algae simulated experiment monitoring system, including:
Culture apparatus 1, for holding the bed mud containing rest period blue-green algae and sample point water body;
Multiple imaging devices 10, are uniformly arranged in outside the side wall of culture apparatus 1 layer by layer, for shooting in culture apparatus 1
The growth image of blue-green algae in different water layers in different time points;
Remote monitoring device 11, is connected with imaging device 10, for receiving and handling the image of the transmission of imaging device 10 simultaneously
It is located at characteristic parameter of the blue-green algae floccule body in different time points of different water layers during acquisition algal bloom.
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, the characteristic parameter of blue-green algae floccule body is blue-green algae
Projected area A, maximum perimeter P and the maximum length L of floccule body.
Further, the outer cover of culture apparatus 1 is provided with outer layer clamping device 2, culture apparatus 1 and outer layer clamping device 2
Space is left between the wall of side, the flowing for water is with the water temperature in regulation culture device 1;Preferably, culture apparatus 1 and outer layer
Distance between the side wall of clamping device 2 is 10~15cm (such as 10.5mm, 11mm, 12mm, 13mm, 14mm, 14.5mm).
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, system also includes circulation water feeder 12,
Space between culture apparatus 1 and the side wall of outer layer clamping device 2 is connected, to supply the water with specified temp into space, more
Preferably, circulation water feeder 12 outlet be connected with the entrance of outer layer clamping device 2, the outlet of outer layer clamping device 2 and
Circulate the entrance connection of water feeder 12.
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, circulation water feeder 12 includes:Tank 5;
Thermal Control Element 53, is arranged on tank 5;Temp probe 6, is arranged in tank 5, for detecting the water temperature in tank 5;Temperature control
Device 7 processed, is arranged on outside tank 5, is connected respectively with temp probe 6 and Thermal Control Element 53, according to the feedack control of temp probe 6
Water temperature in the regulation of Thermal Control Element 53 tank 5 processed;Preferably, the leaving water temperature of tank 5 be 10~40 DEG C (such as 11 DEG C, 15 DEG C,
20℃、25℃、30℃、35℃、39℃).Entrance of the recirculated water through water inlet pipe 3 and outer layer chuck cylinder of 52 outflows is exported from tank
21 flow to the space between the side wall of culture apparatus 1 and outer layer clamping device 2, and then the outlet 22 through outer layer chuck cylinder is flowed out, so
Tank 5 is flowed back to by outlet pipe 4 and tank entrance 51.
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, culture apparatus 1 is the square of top surface opening
Cylinder, culture apparatus 1 is made up of transparent organic glass;Preferably, the wall thickness of culture apparatus 1 be 7-9mm (such as 7.5mm, 8mm,
8.5mm)。
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, outer layer clamping device 2 be top surface opening just
Cube cylinder, outer layer clamping device 2 is made up of transparent PE material or glass;Preferably, the wall thickness of outer layer clamping device 2 is 7-9mm
(such as 7.5mm, 8mm, 8.5mm).
It is highly preferred that black pigment is coated with four perisporiums of outer layer clamping device 2 and the outer surface of bottom wall, so that culture
Four perisporiums and bottom wall of device 1 are closed light tight.By above-mentioned setting, the top of culture apparatus 1 is only set to receive illumination, equivalent to
Culture apparatus 1 is set to the light conditions of the water bodys such as similar Hu Ku.Black pigment is such as blacking.
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, set on four the week side of boss walls of outer layer clamping device 2
Multiple through holes 23 are put, through hole 23 penetrates outer layer clamping device 2 and extended to along the inwall of outer layer clamping device 2 outside culture apparatus 1
Wall;Imaging device 10 is arranged in through hole 23;The size of through hole 23 matches with imaging device 10, the quantity of imaging device 10
It is identical with the quantity of through hole 23;It is highly preferred that imaging device 10 is arranged in through hole 23 by support.
Preferably, it is parallel to each other along circumferential 3 layers of the setting on the side wall of outer layer clamping device 2 of outer layer clamping device 2
Through hole 23, every layer is uniformly arranged in 12 through holes, 23,3 layers of through hole 23, and first layer through hole 23 is disposed in proximity to the bottom of culture apparatus 1
Portion, so that imaging device 10 shoots the image in blue-green algae recovery period;Second layer through hole 23 is arranged in culture apparatus 1 in water body
Portion, so that imaging device 10 photographs the image in blue-green algae floating period;Third layer through hole 23 is arranged at water body in culture apparatus 1
Top, so that imaging device 10 photographs the image that blue-green algae is gathered in water surface formation wawter bloom.
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, imaging device 10 is the micro- number of high-resolution
Code imaging device;The micro- digital imaging devices of high-resolution are preferably 5,000,000 pixel color CMOS progressive scanning picture sensors;
Preferably, remote monitoring device 11 is computer processor;Micro- digital analytical measurement system is provided with computer processor;It is micro-
Digital analytical measurement system is preferably bright U.S. micro- digital imaging system V9.5.2;It is highly preferred that being also set up in computer processor
There are at least one of image mosaic, imaging importing and three-dimensional animation making software.
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, system also includes:Intensity of illumination control dress
Put, be arranged on directly over culture apparatus 1, for providing illumination to culture apparatus 1;Preferably, intensity of illumination control device is by more
Individual fluorescent lamp 8 is formed in parallel, and each fluorescent lamp 8 sets switch, switch one fluorescent lamp 8 of control, by controlling daylight
The unlatching quantity of lamp 8 controls intensity of illumination;
Preferably, the intensity of illumination of intensity of illumination control device be 0~10000lux (such as 100lux, 500lux,
1000lux、2000lux、3000lux、4000lux、5000lux、6000lux、7000lux、8000lux、9000lux、
9500lux、9900lux)。
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, the system also includes ventilation apparatus, and air feed is set
It is standby to include air blower 9 and air speed measuring apparatus;Air blower 9 is arranged on the top of culture apparatus 1, for being provided necessarily to culture apparatus 1
Wind;Preferably, air blower 9 is movable equipment, and air blower 9 is controlled freely to adjust the height and angle of air blower 9
Wind speed and direction;Air speed measuring apparatus is set close to the water surface of culture apparatus 1, the water surface for measuring culture apparatus 1
Wind speed;Preferably, the wind speed of the water surface of culture apparatus 1 be 0~3.5m/s (such as 0.1m/s, 0.5m/s, 1.0m/s,
1.5m/s、2.0m/s、2.5m/s、3.0m/s、3.4m/s)。
Further, in above-mentioned bloom blue algae simulated experiment monitoring system, the N/P ratio of water body is 1 in culture apparatus 1
~40:1.
A kind of bloom blue algae simulated experiment monitoring method, includes successively:
Step one, gathered using imaging device 10 and be located at the blue-green algae of different water layers in culture apparatus 1 in different time points
Growth image, and be sent to remote monitoring device 11;
Step 2, is pre-processed using the first processing system in remote monitoring device 11 to growth image, so that raw
Long image is apparent;
Step 3, using the second processing system in remote monitoring device 11 to the blue-green algae in pretreated growth image
Floccule body carries out analysis measurement, obtains the characteristic parameter of the blue-green algae floccule body in each water layer of different time points, characteristic parameter is
Projected area A, maximum perimeter P and the maximum length L of blue-green algae floccule body;
Step 4, the characteristic parameter of the blue-green algae floccule body obtained according to step 3 calculates algal bloom process according to budget law
In be located at same water layer same image acquisition time point blue-green algae floccule body one dimensional fractal dimension D1, two-dimensional fractal dimension
D2 and three-dimensional fractal dimension D3;
Budget law is specific as follows:
First, positioned at same water layer the projected area A of blue-green algae floccule body of same image acquisition time point, maximum week
The one dimensional fractal dimension D1 of the blue-green algae floccule body of the image acquisition time points of the long P and maximum length L with being located at the water layer,
There is the relation as shown in formula (1)~(3) in two-dimensional fractal dimension D2 and three-dimensional fractal dimension D3:
P∝LD1(1);
A∝LD2Or A ∝ PD2(2);
V∝LD3Or V ∝ PD3(3);
Wherein, V represent blue-green algae floccule body into sphere volume, obtained by the projected area A conversions of blue-green algae floccule body, specific meter
Calculation method is as follows:Try to achieve with the diameter of a circle dp of projected area A homalographics that (i.e. equivalent is straight by the projected area A of blue-green algae floccule body
Footpath), then blue-green algae floccule body is calculated into sphere volume V by diameter dp;
Then, formula (1), (2), (3) both sides are taken into natural logrithm, obtained such as the relational expression of formula (4)~(6):
Ln P=D1ln L+a (4);
Ln A=D2ln P+b=D2ln L+b (5);
Ln V=D3ln P+c=D3ln L+c (6);
Afterwards, with the corresponding ln P of each blue-green algae floccule body of the same image acquisition time point positioned at same water layer with
Ln L values make ln P-ln L linear relation figures, wherein, the slope of straight line is the blue-green algae of the image acquisition time point of the water layer
The one dimensional fractal dimension D1 of floccule body, intercept is constant a;
With the corresponding ln A of each blue-green algae floccule body and ln P of the same image acquisition time point positioned at same water layer
Or ln A make ln A-ln P or ln A-ln L linear relation figures with ln L values, wherein, slope being somebody's turn to do for the water layer of straight line
The two-dimensional fractal dimension D2 of the blue-green algae floccule body of image acquisition time point, intercept is constant b;
With the corresponding Ln V of each blue-green algae floccule body and ln P of the same image acquisition time point positioned at same water layer
Or Ln V make Ln V-ln P or Ln V-ln L linear relation figures with ln L values, wherein, slope being somebody's turn to do for the water layer of straight line
The three-dimensional fractal dimension D3 of the blue-green algae floccule body of image acquisition time point, intercept is constant c.
Further, it is first in above-mentioned bloom blue algae simulated experiment monitoring method, in addition to three-dimensional demonstration animation step
First, the image positioned at same water layer that same image acquisition time point is gathered is subjected to splicing overlap-add procedure, so as to obtain every
The splicing stacking chart of each image acquisition time point of individual water layer;Then, will be each according to the sequencing of image acquisition time
The splicing stacking chart of water layer is fabricated to the three-dimensional demonstration animation that blue-green alga bloom onset process is simulated in different time dimension.It is preferred that
Ground, splicing overlap-add procedure is carried out using softwares such as bright U.S. micro- digital imaging system V5.1, Matlab, Sufer to image.
Further, in above-mentioned bloom blue algae simulated experiment monitoring method, the first processing system is micro- digital analysis
Measuring system;Micro- digital analytical measurement system is preferably bright U.S. micro- digital imaging system V9.5.2.
Further, in above-mentioned bloom blue algae simulated experiment monitoring method, in step 2, pre-process to carry out successively
Except making an uproar, correction process.
Further, in above-mentioned bloom blue algae simulated experiment monitoring method, second processing system is image measurement and divided
Analyse software, the characteristic parameter for measuring and analyzing blue-green algae floccule body.Preferably, second processing system be bright U.S. it is micro- number into
As system V9.5.2.
Further, in above-mentioned bloom blue algae simulated experiment detection method, different time points collection blue algae growth image
Refer to:Start the release aggregation from bed mud in blue-green algae and float preceding every a 1-2h blue algae growth image of shooting, start in blue-green algae
Discharge, assemble when floating every the blue algae growth image of shooting in 5-10 minutes from bed mud.
When simulating the outburst of wawter bloom under nature, it is not necessary to add nutritive salt, different illumination intensity, temperature are directly adjusted
Degree and wind disturbance can just carry out the simulation that the influence factors such as illumination, temperature, wind disturbance trigger blue-green algae recovery algal bloom
Experiment.
When using nutritive salt (only consider nitrogen and phosphorus) as influence factor (influence such as similar illumination, temperature, wind disturbance because
Element) carry out during simulated experiment, water body N/P ratio 1~40 is controlled by adding a certain amount of potassium nitrate, potassium dihydrogen phosphate etc., is opened
Exhibition experiment.
Embodiment 1
Bloom blue algae is simulated and monitored to the present embodiment using bloom blue algae simulated experiment monitoring system as shown in Figure 1
Growth course.
(1) the specific setting of experimental provision is as follows:
In the outer layer tapping of outer layer chuck cylinder, three layers of point upper, middle and lower places 5,000,000 pixel color CMOS progressive scan figures
As sensor, every layer uniform place 12 5,000,000 pixel color CMOS progressive scanning pictures sensors (around outer layer chuck cylinder,
Every layer per 3, face), 36 are placed altogether, and are fixed with support.The pixel color CMOS progressive scanning pictures sensing of lower floor 5,000,000
Cylinder bottom 40cm of the device away from outer layer chuck cylinder, middle level is away from cylinder bottom 80cm, and upper strata is away from cylinder bottom 120cm.The high 1.5m of culture apparatus.The same face
50cm is spaced in same layer between two 5,000,000 pixel color CMOS progressive scanning picture sensors, 5,000,000 pixels of both sides are color
Lateral edges 25cm of the color CMOS progressive scanning pictures sensor away from outer layer chuck cylinder.
(2) when carrying out bloom blue algae simulated experiment, following experimental group experiment is set:
First group, only input have accumulated the bed mud sample of dormancy blue-green algae and the water body of substrate sludge sampling point in culture apparatus;
Group experiment is used as blank group;Specifically, the bed mud sample of the blue-green algae containing dormancy is obtained by the following method:Sampled with column
Device gathers bed mud sample, and 2~5cm of the superiors mud posts are cut, is put into plate and keeps its surface completely to take back laboratory, simultaneously
Sampled point water sample is taken to take back laboratory.Keep bed mud surface complete, note avoiding agitation bed mud surface, be put into confluent cultures device
Bottom (5~10cm of bed mud sample thickness).It is slowly added to pre- first pass through Whatman GF/C filter paper (1.2 μm of φ) mistake along container inner wall
The raw water body water sample (water sample after filtering can ensure that simulated experiment starts in preceding water body without blue-green algae) of algae is filtered out, water body control exists
1.3m it is interior.
Second group, input have accumulated the bed mud sample of dormancy blue-green algae and the water body of substrate sludge sampling point in culture apparatus, and
Apply the nutritive salt such as a certain amount of potassium nitrate, potassium dihydrogen phosphate to be tested, adding the amount of potassium nitrate and potassium dihydrogen phosphate makes water
Nitrogen in body:The mol ratio of phosphorus is 1~40:1 is advisable;The group is tested for testing different N/P ratios in bloom blue algae onset process
Influence;
3rd group, input have accumulated the bed mud sample of dormancy blue-green algae and the water body of substrate sludge sampling point in culture apparatus, so
Different intensities of illumination are set to be tested by opening the quantity of fluorescent lamp afterwards;The group is tested for testing intensity of illumination in water
Influence in Chinese blue algae onset process;
4th group, input have accumulated the bed mud sample of dormancy blue-green algae and the water body of substrate sludge sampling point in culture apparatus, so
The temperature of different recirculated waters is set to be tested afterwards;The group tests the shadow in bloom blue algae onset process for test temperature
Ring;
5th group, input have accumulated the bed mud sample of dormancy blue-green algae and the water body of substrate sludge sampling point in culture apparatus, if
Put different wind-force and wind direction is tested;The group tests the shadow for testing wind-force and wind direction in bloom blue algae onset process
Ring.
(3) the specific method of testing of bloom blue algae simulated experiment is as follows:
Using the onset process of 5,000,000 pixel color CMOS progressive scanning picture Sensor monitoring bloom blue algaes, in blue-green algae
Start to shoot a blue algae growth image every 1 hour before aggregation is floated;When blue-green algae starts release, aggregation floating from bed mud
Depending on 5-10 minutes shoot a blue algae growth image, specific time interval visual exam situation.Constantly monitor within 24 hours
Bloom blue algae breaks out situation, and is layered shooting blue-green algae floating image.
Computer processor is connected with 5,000,000 pixel color CMOS progressive scanning picture sensors and used, 5,000,000 pixels are color
The image information that color CMOS progressive scanning pictures sensor is shot passes to computer processor, and computer processor is received and located
Reason, it is specific as follows:
The first step, is gathered using 5,000,000 pixel color CMOS progressive scanning pictures sensors and is located at difference in culture apparatus
The blue-green algae of water layer and is sent to computer processor in the growth image of different time points;
Second step, using the bright U.S. micro- digital imaging system V9.5.2 in computer processor to growth image line except making an uproar,
The pretreatment such as correction;
3rd step, is schemed using the bright U.S. micro- digital imaging system V9.5.2 in computer processor to pretreated growth
Blue-green algae floccule body as in carries out analysis measurement, obtains the characteristic parameter of the blue-green algae floccule body in each water layer of different time points,
Characteristic parameter is projected area A, maximum perimeter P and the maximum length L of blue-green algae floccule body, and features described above parameter is to calculate point
Shape dimension.
4th step, the characteristic parameter of the blue-green algae floccule body obtained according to the 3rd step calculates algal bloom process according to budget law
In be located at same water layer same image acquisition time point blue-green algae floccule body one dimensional fractal dimension D1, two-dimensional fractal dimension
D2 and three-dimensional fractal dimension D3;
Budget law is specific as follows:
First, positioned at same water layer the projected area A of blue-green algae floccule body of same image acquisition time point, maximum week
Long P and maximum length L and the blue-green algae floccule body of the image acquisition time point positioned at the water layer one dimensional fractal are tieed up
There is the relation as shown in formula (1)~(3) in number D1, two-dimensional fractal dimension D2 and three-dimensional fractal dimension D3:
P∝LD1(1);
A∝LD2Or A ∝ PD2(2);
V∝LD3Or V ∝ PD3(3);
Wherein, V represent the blue-green algae floccule body into sphere volume, converted by the projected area A of the blue-green algae floccule body
Arrive, circular is as follows:Diameter of a circle dp with projected area A homalographics is tried to achieve by the projected area A of blue-green algae floccule body
(i.e. equivalent diameter), then the blue-green algae floccule body is calculated into sphere volume V by diameter dp;
Then, formula (1), (2), (3) both sides are taken into natural logrithm, obtains the relational expression as described in formula (4)~(6):
Ln P=D1ln L+a (4);
Ln A=D2ln P+b=D2ln L+b (5);
Ln V=D3ln P+c=D3ln L+c (6);
Afterwards, with the corresponding ln of each described blue-green algae floccule body of the same image acquisition time point positioned at same water layer
P makees ln P-ln L linear relation figures with ln L values, wherein, the slope of straight line is the image acquisition time point of the water layer
The one dimensional fractal dimension D1 of the blue-green algae floccule body, intercept is constant a;
With the corresponding ln A of each described blue-green algae floccule body of the same image acquisition time point positioned at same water layer with
Ln P or ln A make ln A-ln P or ln A-ln L linear relation figures with ln L values, wherein, the slope of straight line is the water layer
The image acquisition time point the blue-green algae floccule body two-dimensional fractal dimension D2, intercept be constant b;
With the corresponding Ln V of each described blue-green algae floccule body of the same image acquisition time point positioned at same water layer with
Ln P or Ln V make Ln V-ln P or Ln V-ln L linear relation figures with ln L values, wherein, the slope of straight line is the water layer
The image acquisition time point the blue-green algae floccule body three-dimensional fractal dimension D3, intercept be constant c.
5th step, first, using bright U.S. micro- digital imaging system V5.1 to same image acquisition time point gathered
Image positioned at same water layer and on the wall of the same side carries out splicing, then using softwares such as Matlab or Sufer
The stitching image formed respectively on four side walls is overlapped processing, so as to obtain each IMAQ of each water layer
The splicing stacking chart at time point;Then, according to the sequencing of image acquisition time by the splicing stacking chart of each water layer
It is fabricated to the three-dimensional demonstration animation that blue-green alga bloom onset process is simulated in different time dimension.
(5) conclusion:
1. method of the invention can be clearly recognized under different external influence factors, blue in blue-green alga bloom onset process
The whole process of algae floccule body formation, because every kind of blue-green algae has its most suitable N/P, water temperature, light intensity and wind speed, it is possible to logical
The system and method for crossing the present invention determines that its outburst forms the most suitable factor of influence of wawter bloom.Different figures can be calculated using budget law
As one dimensional fractal dimension D1, the two-dimensional fractal dimension D2 of the blue-green algae floccule body of the same water layer of acquisition time and three-dimensional point
Shape dimension D3, the fractal dimension of obtained different time points can be used for the model for setting up bloom blue algae onset process.
2. the image positioned at same water layer same image acquisition time point gathered is soft using Matlab or Sufer etc.
Part carries out splicing overlap-add procedure, obtains the splicing stacking chart of each image acquisition time point of each water layer;Then, according to figure
Blue algae water is simulated as the splicing stacking chart of each water layer is fabricated in different time dimension by the sequencing of acquisition time
The three-dimensional demonstration animation of magnificent onset process.
Claims (10)
1. a kind of bloom blue algae simulated experiment monitoring system, it is characterised in that including:
Culture apparatus, for holding the bed mud containing rest period blue-green algae and sample point water body;
Multiple imaging devices, are uniformly arranged in outside the side wall of the culture apparatus layer by layer, for shooting in the culture apparatus
The growth image of blue-green algae in different water layers in different time points;
Remote monitoring device, is connected with the imaging device, for receiving and handling the image of the imaging device transmission and obtain
Be located at characteristic parameter of the blue-green algae floccule body in different time points of different water layers during algal bloom.
2. bloom blue algae simulated experiment monitoring system according to claim 1, it is characterised in that the blue-green algae floccule body
Characteristic parameter is projected area A, maximum perimeter P and the maximum length L of blue-green algae floccule body.
3. bloom blue algae simulated experiment monitoring system according to claim 1, it is characterised in that outside the culture apparatus
It is arranged with outer layer clamping device, leaves space between the side wall of the culture apparatus and the outer layer clamping device, the stream for water
Move to regulate and control the water temperature in the culture apparatus;Preferably, the side wall of the culture apparatus and the outer layer clamping device
Between distance be 10~15cm;
Preferably, the culture apparatus is the square cylinder of top surface opening, and the culture apparatus is made up of transparent organic glass;It is excellent
Selection of land, the wall thickness of the culture apparatus is 7-9mm;Preferably, the N/P ratio of water body is 1~40 in the culture apparatus:1;
Preferably, the outer layer clamping device is the square cylinder of top surface opening, and the outer layer clamping device is by transparent PE material
Or glass is made;Preferably, the wall thickness of the outer layer clamping device is 7-9mm;
It is highly preferred that black pigment is coated with four perisporiums of the outer layer clamping device and the outer surface of bottom wall, so that described
Four perisporiums and bottom wall of culture apparatus are closed light tight.
4. the bloom blue algae simulated experiment monitoring system according to any one of claims 1 to 3, it is characterised in that the system
System also includes circulation water feeder, and the space between the culture apparatus and the outer layer clamping device side wall connects, with to
Water of the supply with specified temp in the space, it is highly preferred that the outlet of the circulation water feeder is pressed from both sides with the outer layer
The entrance connection of covering device, the outlet of the outer layer clamping device is connected with the entrance of the circulation water feeder;
It is further preferred that the circulation water feeder includes:Tank;Thermal Control Element, is arranged on the tank;Temperature is visited
Head, is arranged in the tank, for detecting the water temperature in the tank;Temperature controller, is arranged on outside the tank, respectively
It is connected with the temp probe and the Thermal Control Element, controls the Thermal Control Element to adjust according to the temp probe feedack
Save the water temperature in the tank;Preferably, the leaving water temperature of the tank is 10~40 DEG C.
5. the bloom blue algae simulated experiment monitoring system according to any one of Claims 1 to 4, it is characterised in that described
Multiple through holes are set on four the week side of boss walls of outer layer clamping device, and through hole penetrates outer layer clamping device and along along outer layer clamping device
Wall extends to the culture apparatus outside wall surface;Imaging device is arranged in through hole;The size of the through hole and the imaging device
Match, the quantity of the imaging device is identical with the quantity of the through hole;It is highly preferred that the imaging device is set up by branch
Put in the through hole;
Preferably, it is parallel to each other along circumferential 3 layers of the setting on the side wall of the outer layer clamping device of the outer layer clamping device
Through hole, every layer is uniformly arranged in 12 through holes, 3 layers of through hole, and first layer through hole is disposed in proximity to the culture apparatus
Bottom, so that the imaging device shoots the image in blue-green algae recovery period;Second layer through hole is arranged at the culture apparatus reclaimed water
The middle part of body, so that the imaging device photographs the image in blue-green algae floating period;Third layer through hole is arranged at the culture dress
The top of middle water body is put, so that the imaging device photographs the image that blue-green algae is gathered in water surface formation wawter bloom;
It is highly preferred that the imaging device is the micro- digital imaging devices of high-resolution;The micro- digital image-forming of high-resolution
Device is preferably 5,000,000 pixel color CMOS progressive scanning picture sensors;Preferably, the remote monitoring device is at computer
Manage device;Micro- digital analytical measurement system is provided with the computer processor;The micro- digital analytical measurement system is preferred
For bright U.S. micro- digital imaging system V9.5.2;Folded it is highly preferred that being additionally provided with image mosaic, image in the computer processor
At least one of plus with three-dimensional animation making software.
6. the bloom blue algae simulated experiment monitoring system according to any one of Claims 1 to 5, it is characterised in that the system
System also includes:Intensity of illumination control device, is arranged on directly over the culture apparatus, for providing light to the culture apparatus
According to;Preferably, the intensity of illumination control device is formed in parallel by multiple fluorescent lamps, and each fluorescent lamp is set out
Close, switch one fluorescent lamp of control, by controlling the unlatching quantity of the fluorescent lamp to control intensity of illumination;
Preferably, the intensity of illumination of the intensity of illumination control device is 0~10000lux.
7. the bloom blue algae simulated experiment monitoring system according to any one of claim 1~6, it is characterised in that the system
Also include ventilation apparatus, the ventilation apparatus include air blower and air speed measuring apparatus;The air blower is arranged on the culture dress
Top is put, for providing certain wind to culture apparatus;Preferably, the air blower is movable equipment, freely to adjust institute
The height and angle of air blower is stated to control the wind speed and direction of air blower;The air speed measuring apparatus is close to the culture apparatus
Water surface is set, the wind speed of the water surface for measuring the culture apparatus;Preferably, the water body table of the culture apparatus
The wind speed in face is 0~3.5m/s.
8. a kind of bloom blue algae simulated experiment monitoring method, it is characterised in that include successively:
Step one, growth figure of the blue-green algae in different time points for being located at different water layers in culture apparatus is gathered using imaging device
Picture, and it is sent to remote monitoring device;
Step 2, is pre-processed using the first processing system in the remote monitoring device to the growth image, so that
The growth image is apparent;
Step 3, using the second processing system in the remote monitoring device to the indigo plant in the pretreated growth image
Algae floccule body carries out analysis measurement, obtains the characteristic parameter of the blue-green algae floccule body in each water layer of different time points, the feature
Parameter is projected area A, maximum perimeter P and the maximum length L of blue-green algae floccule body;
Step 4, the characteristic parameter of the blue-green algae floccule body obtained according to step 3 calculates algal bloom process according to budget law
In be located at same water layer same image acquisition time point blue-green algae floccule body one dimensional fractal dimension D1, two-dimensional fractal dimension
D2 and three-dimensional fractal dimension D3;
The budget law is specific as follows:
First, positioned at same water layer the projected area A of the blue-green algae floccule body of same image acquisition time point, maximum week
Long P and maximum length L and the blue-green algae floccule body of the image acquisition time point positioned at the water layer one dimensional fractal are tieed up
There is the relation as shown in formula (1)~(3) in number D1, two-dimensional fractal dimension D2 and three-dimensional fractal dimension D3:
P∝LD1(1);
A∝LD2Or A ∝ PD2(2);
V∝LD3Or V ∝ PD3(3);
Wherein, V represent the blue-green algae floccule body into sphere volume, obtained, had by the projected area A conversions of the blue-green algae floccule body
Body computational methods are as follows:Diameter of a circle dp with projected area A homalographics is tried to achieve by the projected area A of the blue-green algae floccule body
(i.e. equivalent diameter), then the blue-green algae floccule body is calculated into sphere volume V by diameter dp;
Then, formula (1), (2), (3) both sides are taken into natural logrithm, obtains the relational expression as described in formula (4)~(6):
Ln P=D1ln L+a (4);
Ln A=D2ln P+b=D2ln L+b (5);
Ln V=D3ln P+c=D3ln L+c (6);
Afterwards, with the corresponding ln P of each described blue-green algae floccule body of the same image acquisition time point positioned at same water layer with
Ln L values make ln P-ln L linear relation figures, wherein, the slope of straight line is the described of the image acquisition time point of the water layer
The one dimensional fractal dimension D1 of blue-green algae floccule body, intercept is constant a;
With each described blue-green algae floccule body corresponding lnA and ln P of the same image acquisition time point positioned at same water layer or
Ln A make ln A-ln P or ln A-ln L linear relation figures with ln L values, wherein, the slope of straight line is the figure of the water layer
As the two-dimensional fractal dimension D2 of the blue-green algae floccule body of acquisition time, intercept is constant b;
With each described blue-green algae floccule body corresponding LnV and ln P of the same image acquisition time point positioned at same water layer or
Ln V make Ln V-ln P or Ln V-ln L linear relation figures with ln L values, wherein, the slope of straight line is the figure of the water layer
As the three-dimensional fractal dimension D3 of the blue-green algae floccule body of acquisition time, intercept is constant c.
9. bloom blue algae simulated experiment monitoring method according to claim 8, it is characterised in that also dynamic including three-dimensional demonstration
Making step is drawn, first, the image positioned at same water layer that same image acquisition time point is gathered is carried out at splicing superposition
Reason, so as to obtain the splicing stacking chart of each image acquisition time point of each water layer;Then, according to image acquisition time
The splicing stacking chart of each water layer is fabricated in different time dimension and simulates blue-green alga bloom onset process by sequencing
Three-dimensional demonstration animation.
10. bloom blue algae simulated experiment monitoring method according to claim 8, it is characterised in that first processing system
Unite as micro- digital analytical measurement system;The micro- digital analytical measurement system is preferably bright U.S. micro- digital imaging system
V9.5.2;Preferably, in the step 2, the pretreatment is carries out except making an uproar successively, correction process;Preferably, described
Two processing systems are image measurement and analysis software, the characteristic parameter for measuring and analyzing blue-green algae floccule body;Preferably, it is described
Second processing system is bright U.S. micro- digital imaging system V9.5.2;Preferably, the different time points collection blue algae growth figure
Seem to refer to:Start the release aggregation from bed mud in blue-green algae and float preceding every a 1-2h blue algae growth image of shooting, opened in blue-green algae
Begin to discharge from bed mud, assemble when floating every the blue algae growth image of shooting in 5-10 minutes.
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CN107102003B (en) | 2023-08-11 |
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