CN107290485A - The controllable intertidal zone CO of environmental condition2Flux simulating lab test device and method - Google Patents
The controllable intertidal zone CO of environmental condition2Flux simulating lab test device and method Download PDFInfo
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- 230000007613 environmental effect Effects 0.000 title claims abstract description 29
- 238000009533 lab test Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims description 28
- 238000005070 sampling Methods 0.000 claims abstract description 35
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 25
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 16
- 238000003860 storage Methods 0.000 claims abstract description 12
- 239000003673 groundwater Substances 0.000 claims abstract description 8
- 230000035699 permeability Effects 0.000 claims abstract description 4
- 230000008569 process Effects 0.000 claims description 19
- 238000005259 measurement Methods 0.000 claims description 18
- 230000004907 flux Effects 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 238000012544 monitoring process Methods 0.000 claims description 15
- 238000004088 simulation Methods 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 8
- 239000013535 sea water Substances 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 238000007689 inspection Methods 0.000 claims description 4
- 239000004745 nonwoven fabric Substances 0.000 claims description 4
- 238000012417 linear regression Methods 0.000 claims description 3
- 238000009738 saturating Methods 0.000 claims description 3
- 230000001131 transforming effect Effects 0.000 claims description 3
- 238000004177 carbon cycle Methods 0.000 abstract description 8
- 238000011160 research Methods 0.000 abstract description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 description 24
- 239000007789 gas Substances 0.000 description 24
- 239000002689 soil Substances 0.000 description 21
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 12
- 230000008859 change Effects 0.000 description 11
- 230000029058 respiratory gaseous exchange Effects 0.000 description 7
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- 238000002474 experimental method Methods 0.000 description 4
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- 229920005439 Perspex® Polymers 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
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- 235000019256 formaldehyde Nutrition 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
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Abstract
The invention discloses a kind of controllable intertidal zone CO of environmental condition2Flux simulating lab test device, including biochemical cultivation case, outer barrel and storage tank, the outer barrel is located in biochemical cultivation case, and the deposit sample that layering in inner cylinder, inner cylinder is put into field acquisition is provided with outer barrel, permeability hole is provided with inner cylinder bottom, permeable hole is provided with inner cylinder, permeable hole is located at the top of deposit sample, and outer barrel is connected by peristaltic pump with storage tank, provided with sampling air chamber above the deposit sample of inner cylinder, sampling air chamber passes through gas circuit pipeline and CO2Sensor is connected, CO2Sensor is connected with pcs signal.A kind of controllable intertidal zone CO of environmental condition of the invention2Flux simulating lab test device, various temperature, photoenvironment condition and level of ground water can be simulated indoors, carry out the intertidalite CO of long-time Continuous Observation2Flux, strong observation and research meanses are provided for basic research such as wetlands ecosystems carbon metablism, carbon cycles.
Description
Technical field
The present invention relates to the controllable intertidal zone CO of environmental condition2Flux simulating lab test device and method, belongs to environment
Field.
Background technology
Wetlands ecosystems are the important carbon storehouses of the earth, account for the 10% of Global Terrestrial Ecosystem carbon storehouse, be at present
Know that the important carbon that forest is only second in terrestrial ecosystems converges, the carbon cycle on global range is significantly affected.Have in wetland
The incomplete decomposing of machine matter causes the accumulation of carbon in wetland, and wetland plant obtains substantial amounts of CO from air2, as huge carbon
Storehouse；Meanwhile, certain in the period of and under the conditions of, wetland is in the presence of microorganism by decomposing and respiration is by CO2Discharge
Into air, the carbon cycle process climate condition of wetland and the influence of mankind's activity have played " carbon remittance " and " carbon source " two side
The effect in face.Intertidal zone is the class in numerous kinds of wetlands, is the handing-over area of land and ocean.In recent years, it is " blue as the whole world
Color carbon converge " significant contributor, have high vegetative coverage intertidal zone wetland (mangrove swamp and sabkha) carbon cycle by
Great concern.In addition to the intertidal zone primary productivity high except possessing and low organic matter decomposition speed, intertidal zone wetland
Vegetation root/shoot ratio is larger, can store substantial amounts of carbon and transfer to be stored in soil by root system week.Meanwhile, estuary coast is unique
Sedimentation process a large amount of endogenous or external source organic carbons can quickly be buried.It can be seen that, intertidal zone muskeg productivity is high, machine
Carbon decomposition rate is low, CH4 discharges are weaker, Carbon deposition speed is fast, is one of unit area carbon sequestration speed highest ecosystem,
There is significant ability to pool carbon.According to statistics, in global range total carbon sequestration speed of seashore wetland more than 100Tg.a-1(with carbon
Meter), therefore intertidal zone wetland has important potentiality in terms of carbon containing greenhouse gas emission, reduction Global Greenhouse Effect is slowed down.
Influence the factor of intertidal zone soil carbon dioxide flux change a lot, including：Hydrologic condition, the soil moisture, micro- life
Thing activity, organic matter and phytoplankton content etc..CO in the ecosystem under different vegetative coverages2Burst size it is different, and
Change with diurnal variation and seasonality change.Kurganova etc. has found soil CO2Discharge is concentrated mainly on summer and autumn,
Also, degree of fluctuation summer of the system respiration efflux in one day on different time is significantly greater than winter.Van Der Nat etc. are surveyed
The intraday system respiration efflux of amount typically exhibits the trend of single peak type change, slowly rises since morning, to going out in the afternoon
It is slow again after existing maximum to decline.Wetland soil discharges CO to air2Process master be Soil Microorganism oxidation Decomposition make
With and root system of plant respiration.According to correlative study, the CO discharged in soil2It is to come from the micro- life of soil to have 85%-90%
The vital movement of thing, 15% respiration from root system of plant, pole is partially the lung ventilator chemical oxidation of soil animal.Soil
Never Tongfang face rings CO to earth2Release, most important factor is the temperature of soil.Activity, the plant growth rate of microorganism
Speed all changed by the soil moisture and influenceed.The heterotrophic respiration of microorganism, the autotrophic respiration of plant, the decomposition of organic matter is equal
Influenceed by the soil moisture, temperature is higher, decomposition rate is faster.Buchmann have studied the pass of respiratory rate and the soil moisture
System, as a result shows that soil speed can produce obvious change with the change of the soil moisture.Wetland is in the area of land and water boundary,
Change to hydrologic condition is very sensitive.The Drought-wet change of wetlands ecosystems, can influence the activity of Soil Microorganism.
China's Wetland Area is about 65.94 × 104km2(not including rivers, pond), occupies first, Asia, the world the 4th
Position, wherein natural bioremediation is about 25.94 × 104km2, including Marsh Wetland about 11.97 × 104km2, Tideland resources about 2.17
×104km2, the shallow water along the coast 2.7 × 104km2Deng；Artificial swamp 40.0 × 104km2.Intertidal zone is distributed widely in China coast area
Domain, due to intertidal zone CO2The field survey of flux rate of discharge by the environmental conditions such as meteorology, the hydrology, hydrodynamic force restrict (tide,
Wave and ocean current), measuring environment is harsh and changeable, traditional inland wetland CO2Flux measurement method is difficult to effective implementation, and grows
The research and development of intertidal zone Carbon flux determining instrument and equipment relatively lag behind since phase, the presence of above problems, at present urgently
The research carried out in a deep going way brings very big inconvenience.The research for estuary coast wetland carbon cycle largely uses in situ both at home and abroad
Observation method, this is significant for understanding the ecosystem feature under real environmental conditions.But external environment is changeable
And height is uncertain, particularly with the changeable environment of physical power as intertidal zone, in-situ observation is difficult to true seizure
The feature of carbon cycle under different control conditions, it is more difficult in the wild by serial experiment understand carbon cycle mechanism and control because
Son, is significantly in influence of the experiment lab simulation natural conditions change to Carbon flux therefore.Under experimental conditions,
Factor of influence is controllable, can measure Soil-air interface carbon dioxide flux, more by simulating under different temperatures and moisture condition
Be conducive to further investigation.
The content of the invention
Goal of the invention：In order to overcome the deficiencies in the prior art, the present invention provides a kind of environmental condition controllable tide
Between band CO2Flux simulating lab test device and method, various temperature, photoenvironment condition and underground can be simulated indoors
Water level, carries out the intertidalite CO of long-time Continuous Observation2Flux.
Technical scheme：To achieve the above object, the controllable intertidal zone CO of environmental condition of the invention2Flux lab simulation is real
Experiment device, including biochemical cultivation case, outer barrel and storage tank, the outer barrel are located in biochemical cultivation case, and inner cylinder is provided with outer barrel, interior
Layering is put into the deposit sample of field acquisition in cylinder, and permeability hole is provided with inner cylinder bottom, and permeable hole is provided with inner cylinder, permeable
Hole position is connected in the top of deposit sample, outer barrel by peristaltic pump with storage tank, is provided with above the deposit sample of inner cylinder
Sampling air chamber, sampling air chamber passes through gas circuit pipeline and CO2Sensor is connected, CO2Sensor is connected with pcs signal.
Preferably, being provided with valve between the peristaltic pump and outer barrel.
Preferably, the inner cylinder bottom is provided with non-woven fabrics bed course, permeable and impermeable sand simulates intertidal zone groundwater level
Change.
Preferably, being provided with rotating vane in the sampling air chamber, rotating vane is connected with power supply, and rotating vane is bilayer
Rotating vane, it is ensured that the gas mixing in sampling air chamber is uniform, reduces measurement error.
Preferably, the barrel of the outer barrel is provided with scale, inner cylinder and outer barrel use clear perspex material system
Make, outer barrel edge indicates graduation mark, is easy to read and controls inner cylinder groundwater level.
Preferably, the thief hatch of the sampling air chamber sets detachable stainless steel filtering net, prevent when sampling air chamber
Blade rotational agitation silt enters gas channels, causes mechanical disorder or measurement error, also allows for cleaning.
Preferably, the gas circuit pipeline is provided with drying and dehydrating device, it can prevent that moisture is to CO in gas2What measurement was produced
Influence, improves measurement accuracy.
Preferably, the CO2Sensor is connected by circuit lines with sampling air chamber, passes through gas circuit pipeline and loop pipe
Road constitutes a return passage, and the gas circulation after detection enters in measuring system, it is ensured that CO2Concentration measurement is not by the external world
Environmental disturbances.
Preferably, the bottom outer of the inner cylinder is provided with cushion block, make to be not directly contacted between inner cylinder and outer barrel, realize sea
Water is from bottom even and free exchange.
A kind of controllable intertidal zone CO of above-mentioned environmental condition2The measuring method of flux simulating lab test device, including
Following steps：
(1) from field acquisition intertidalite sample, layering is packed into device inner cylinder, it is ensured that mud face is slightly below internal barrel
Wall permeable hole, a certain amount of sampled point raw water is entered in outer wound packages, is taken back laboratory and is carried out Carbon flux measurement, while collection is enough
Sampled point seawater is used to carry out tide process simulation indoors；
(2) the deposit sample of field acquisition is placed in biochemical cultivation case, the temperature and humidity for adjusting incubator is extremely set
Value, makes the planktonic organism in deposit and seawater gradually adapt to the environmental condition manually built；
(3) outer barrel, peristaltic pump are connected with storage tank by waterway pipe, start peristaltic pump, simulation oceanic tide is made
Flood tide or ebb tide process, adjust wriggling pump discharge, and controlling water level rises or falls speed and is consistent with field inspection result；
(4) sampling air chamber is kept flat to deposit surface and is compacted, connect gas circuit pipeline, check the airtight of air-channel system
Property；
(5) CO is opened2Sensor, continuously measures the CO of deposit surface under the conditions of different levels of ground water2Real-time concentration；
(6) CO is utilized2Concentration process data, intertidalite CO is calculated by below equation2Flux and measurement error：
In formula：For CO2The slope of concentration-time process, ppm/s, tiFor the monitoring time of i-th of Monitoring Data, unit
For s；yiFor the CO of i-th of Monitoring Data2Concentration value, unit is ppm；N is the sum of Monitoring Data；φ is CO2Flux, unit
For mol/m2/d；K is transforming factor；P is gas atmosphere, and unit is Pa；V is sampling chamber volume, and unit is m3；R is general gas
Body constant, 8.314472m3·Pa/mol/K；T is gas temperature, and unit is K；A is area at sampling chamber inlet, and unit is
m2；
CO is calculated using below equation2The evaluated error of flux measurement：
In formula：E is experimental error, calculates data selection and determines the data after starting two minutes, if e is more than 0.9, shows reality
Test the quality of data very well, CO2Preferably, experimental data is with a high credibility for the linear relationship of concentration process, if e is less than 0.5, shows experiment
The quality of data is poor, it is necessary to carry out monitoring again.
In the present invention, on the one hand, the water in outer barrel slowly penetrates or discharged inner cylinder by inner cylinder bottom percolation hole, simulate
The rise or landing of deposit groundwater level during flood tide or ebb tide.On the other hand, with the lifting of outer barrel water level or falling
To deposit surface, water flows in or out inner cylinder, simulation flood tide later stage or ebb tide initial depositing stage thing by inner cylinder barrel permeable hole
The waterflooding on surface and exposure process.By controlling the temperature and intensity of illumination of biochemical cultivation case, simulation different temperatures and illumination feelings
The situation of change of soil carbon flux under condition.Sampling air chamber cover is pressed in deposit surface, rotating vane and CO is opened2Sensor
Switch, measures the CO of Sediment Interface2Concentration, can be to CO by computer2Concentration is monitored in real time.
Beneficial effect：The controllable intertidal zone CO of environmental condition of the invention2Flux simulating lab test device, by this survey
The soil moisture and humidity control system in device and the mutual cooperation of soil flux measurement system are measured, field is solved and surveys on the spot
Measure soil CO2The problem of flux is easily restricted by environmental conditions such as meteorology, the hydrology, hydrodynamic forces, realizes automaticity height, behaviour
Work is easy reliably, can build various temperature and intensity of illumination environment indoors, carries out the purpose long lasting for measurement, is wet
The basic research such as ground ecosystem carbon metablism, carbon cycle provide strong observation and research meanses.
Brief description of the drawings
Fig. 1 is the general structure schematic diagram of apparatus of the present invention.
Fig. 2 is the agent structure schematic diagram of apparatus of the present invention.
Fig. 3 is the elevation and top view of apparatus of the present invention agent structure.
Fig. 4 is apparatus of the present invention outer tube structure schematic diagram.
Fig. 5 is apparatus of the present invention inner tube structure schematic diagram.
Fig. 6 is apparatus of the present invention inner cylinder polycrystalline substance schematic diagram.
Fig. 7 is apparatus of the present invention Carbon flux measuring system schematic diagram.
In figure：1- biochemical cultivation cases；2-CO2Sensor；3- gas circuit pipelines；4- drying and dehydrating devices；5- power supplys；6- is removable
Unload formula stainless steel filtering net；7- sampling air chambers；8- rotating vanes；9- waterway pipes；10- valves；11- peristaltic pumps；12- inner cylinders；13-
Outer barrel；14- non-woven fabrics bed courses；15- cushion blocks；16- storage tanks, 17- computers, 18- inner cylinder barrel permeable holes；19- inner cylinders bottom is saturating
Water hole；20- graduation marks；21- handles.
Embodiment
The present invention is further described below in conjunction with the accompanying drawings.
As shown in Figures 1 to 6, the controllable intertidal zone CO of environmental condition of the invention2Flux simulating lab test device, bag
Biochemical cultivation case 1, outer barrel 13 and storage tank 16 are included, the outer barrel 13 is located in biochemical cultivation case 1, and inner cylinder is provided with outer barrel 13
12, the interior layering of inner cylinder 12 is put into the deposit sample of field acquisition, is provided with permeability hole 19 in the bottom of inner cylinder 12, is set on inner cylinder 12
There is permeable hole 18, permeable hole 18 is located at the top of deposit sample, and outer barrel 13 is connected by peristaltic pump 11 with storage tank 16, wriggles
The flood tide or ebb tide process of the simulation oceanic tide effect of pump 11, adjust the flow of peristaltic pump 11, controlling water level rises or falls speed
It is consistent with field inspection result, waterway pipe 9 is provided between peristaltic pump 11 and outer barrel 13, waterway pipe 9 is provided with valve 10,
Provided with sampling air chamber 7 above the deposit sample of inner cylinder 12, handle 21 is installed on sampling air chamber 7, sampling air chamber 7 passes through gas circuit
Pipeline is connected with CO2 sensors 2, and CO2 sensors 2 are connected with the signal of computer 17.
In the present invention, the bottom of inner cylinder 12 is provided with non-woven fabrics bed course 14, and permeable and impermeable sand simulates intertidal zone underground
Water SEA LEVEL VARIATION.Rotating vane 8 is provided with the sampling air chamber 7, rotating vane 8 is connected with power supply 5, rotating vane 8 is bilayer
Rotating vane, it is ensured that the gas mixing in sampling air chamber 7 is uniform, reduces measurement error.The barrel of the outer barrel 13 is provided with quarter
Degree, inner cylinder 12 and outer barrel 13 are made using clear perspex material, and the edge of outer barrel 13 indicates graduation mark 20, be easy to read and
Control the groundwater level of inner cylinder 12.The thief hatch of the sampling air chamber 7 sets detachable stainless steel filtering net 6, prevents when sampling
The blade rotational agitation silt of air chamber 7 enters gas channels, causes mechanical disorder or measurement error, also allows for cleaning.The gas
Road pipeline 3 is provided with drying and dehydrating device 4, can prevent that moisture is to CO in gas2The influence produced is measured, measurement accuracy is improved.Institute
State CO2 sensors 2 to be connected with sampling air chamber 7 by circuit lines, constituting a loop by gas circuit pipeline 3 and circuit lines leads to
Road, the gas circulation after detection enters in measuring system, it is ensured that CO2Concentration measurement is not by external environmental interference.In described
The bottom outer of cylinder 12 is provided with cushion block 15, makes to be not directly contacted between inner cylinder 12 and outer barrel 13, realize seawater from bottom even and
Free exchange.
In the present invention, inner cylinder 12 and the making material of outer barrel 13, the internal diameter of outer barrel 13 are used as from transparent organic glass
300mm, the internal diameter 200mm of inner cylinder 12 or so.Gas channels, internal diameter selection 5-8mm are done using PVC material.The bottom of inner cylinder 12 and side
Wall small aperture 2mm is advisable, cylinder bottom aperture center of circle spacing 4-5mm, barrel aperture center of circle spacing 50mm.The covering of the bottom of inner cylinder 12 is saturating
Water nonwoven, it is ensured that permeable impermeable sand.Circular detachable formula stainless steel filtering net 6, diameter are used at the sampling sample inlet of air chamber 7
10mm。
A kind of controllable intertidal zone CO of above-mentioned environmental condition2The measuring method of flux simulating lab test device, including
Following steps：
(1) from field acquisition intertidalite sample, layering is packed into device inner cylinder 12, it is ensured that mud face is slightly below inner cylinder
12 barrel permeable holes 18, load a certain amount of sampled point raw water in outer barrel 13, take back laboratory and carry out Carbon flux measurement, adopt simultaneously
The enough sampled point seawater of collection are used to carry out tide process simulation indoors；
(2) the deposit sample of field acquisition is placed in biochemical cultivation case 1, the temperature and humidity for adjusting incubator is extremely set
Value, makes the planktonic organism in deposit and seawater gradually adapt to the environmental condition manually built；
(3) outer barrel 13, peristaltic pump 11 are connected with storage tank 16 by waterway pipe, start peristaltic pump 11, simulation sea
The flood tide or ebb tide process of foreign tidal action, adjust the flow of peristaltic pump 11, and controlling water level rises or falls speed and field inspection
As a result it is consistent；
(4) sampling air chamber 7 is kept flat to deposit surface and is compacted, connection gas circuit pipeline 3 checks the airtight of air-channel system
Property；
(5) CO2 sensors 2 are opened, the CO of deposit surface under the conditions of different levels of ground water is continuously measured2Real-time concentration；
(6) CO is utilized2Concentration process data, intertidal zone Soil-air interface CO is calculated by below equation2Flux：
In formula：For CO2The slope of concentration-time process, ppm/s, tiFor the monitoring time of i-th of Monitoring Data, unit
For s；yiFor the CO of i-th of Monitoring Data2Concentration value, unit is ppm；N is the sum of Monitoring Data；φ is CO2Flux, unit
For mol/m2/d；K is transforming factor；P is gas atmosphere, and unit is Pa；V is the sampling volume of air chamber 7, and unit is m3；R is general
Gas constant, 8.314472m3·Pa/mol/K；T is gas temperature, and unit is K；A is the sampling porch area of air chamber 7, unit
For m2；
(7) CO is calculated using below equation2The linear regression error e of flux measurement：
In formula：E is the linear regression error of experimental data, calculates data selection and determines the data after starting two minutes.
(8) if e is more than 0.9, experimental data is shown very well, CO2Preferably, experimental data is credible for the linear relationship of concentration process
Degree is high, if e is less than 0.5, shows that experimental data is second-rate, it is necessary to carry out monitoring again.
Described above is only the preferred embodiment of the present invention, it should be pointed out that：For the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of controllable intertidal zone CO of environmental condition2Flux simulating lab test device, it is characterised in that：Including biochemical culture
Case, outer barrel and storage tank, the outer barrel are located in biochemical cultivation case, and inner cylinder is provided with outer barrel, and the interior layering of inner cylinder is put into field and adopted
The deposit sample of collection, permeability hole is provided with inner cylinder bottom, and permeable hole is provided with inner cylinder, and permeable hole is located at deposit sample
Top, outer barrel is connected by peristaltic pump with storage tank, and provided with sampling air chamber above the deposit sample of inner cylinder, sampling air chamber leads to
Cross gas circuit pipeline and CO2Sensor is connected, CO2Sensor is connected with pcs signal.
2. the controllable intertidal zone CO of environmental condition according to claim 12Flux simulating lab test device, its feature exists
In：Valve is provided between the peristaltic pump and outer barrel.
3. the controllable intertidal zone CO of environmental condition according to claim 12Flux simulating lab test device, its feature exists
In：The inner cylinder bottom is provided with non-woven fabrics bed course.
4. the controllable intertidal zone CO of environmental condition according to claim 12Flux simulating lab test device, its feature exists
In：Rotating vane is provided with the sampling air chamber, rotating vane is connected with power supply.
5. the controllable intertidal zone CO of environmental condition according to claim 12Flux simulating lab test device, its feature exists
In：The barrel of the outer barrel is provided with scale.
6. the controllable intertidal zone CO of environmental condition according to claim 12Flux simulating lab test device, its feature exists
In：The thief hatch of the sampling air chamber sets detachable stainless steel filtering net.
7. the controllable intertidal zone CO of environmental condition according to claim 12Flux simulating lab test device, its feature exists
In：The gas circuit pipeline is provided with drying and dehydrating device.
8. the controllable intertidal zone CO of environmental condition according to claim 12Flux simulating lab test device, its feature exists
In：The CO2Sensor is connected by circuit lines with sampling air chamber, and a loop is constituted by gas circuit pipeline and circuit lines
Passage.
9. the controllable intertidal zone CO of environmental condition according to claim 12Flux simulating lab test device, its feature exists
In：The bottom outer of the inner cylinder is provided with cushion block.
10. a kind of controllable intertidal zone CO of environmental condition as described in any one of claim 1 to 92Flux simulating lab test is filled
The measuring method put, it is characterised in that comprise the following steps：
(1) from field acquisition intertidalite sample, layering is packed into device inner cylinder, it is ensured that it is saturating that mud face is slightly below inner cylinder barrel
Water hole, a certain amount of sampled point raw water is entered in outer wound packages, is taken back laboratory and is carried out Carbon flux measurement, while the enough samplings of collection
Point seawater is used to carry out tide process simulation indoors；
(2) the deposit sample of field acquisition is placed in biochemical cultivation case, adjusts the temperature and humidity of incubator to setting value, make
Planktonic organism in deposit and seawater gradually adapts to the environmental condition manually built；
(3) outer barrel, peristaltic pump are connected with storage tank by waterway pipe, start peristaltic pump, simulation oceanic tide effect
Flood tide or ebb tide process, adjust wriggling pump discharge, and controlling water level rises or falls speed and is consistent with field inspection result；
(4) sampling air chamber is kept flat to deposit surface and is compacted, connect gas circuit pipeline, check the air-tightness of air-channel system；
(5) CO is opened2Sensor, continuously measures the CO of deposit surface under the conditions of different levels of ground water2Real-time concentration；
(6) CO is utilized2Concentration process data, intertidal zone Soil-air interface CO is calculated by below equation2Flux：
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In formula：For CO2The slope of concentration-time process, ppm/s, tiFor the monitoring time of i-th of Monitoring Data, unit is s；yi
For the CO of i-th of Monitoring Data2Concentration value, unit is ppm；N is the sum of Monitoring Data；φ is CO2Flux, unit is mol/
m2/d；K is transforming factor；P is gas atmosphere, and unit is Pa；V is sampling chamber volume, and unit is m3；R is that argoshield is normal
Number, 8.314472m3·Pa/mol/K；T is gas temperature, and unit is K；A is area at sampling chamber inlet, and unit is m2；
(7) CO is calculated using below equation2The linear regression error e of flux measurement：
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(8) if e is more than 0.9, experimental data quality is shown very well, CO2Preferably, experimental data is credible for the linear relationship of concentration process
Degree is high, if e is less than 0.5, shows that experimental data is second-rate, it is necessary to carry out monitoring again.
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| CN111323548A (en) * | 2020-04-14 | 2020-06-23 | 吕笑非 | Culture box for sea-land interlaced zone ecosystem, control method and application |
| CN112136735A (en) * | 2020-09-04 | 2020-12-29 | 中国科学院海洋研究所 | Method for improving oyster respiration rate stability |
| CN112136735B (en) * | 2020-09-04 | 2022-07-05 | 中国科学院海洋研究所 | Method for improving oyster respiration rate stability |
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Application publication date: 20171024 Assignee: Nanjing Water Energy Automation Technology Co.,Ltd. Assignor: HOHAI University Contract record no.: X2021320000018 Denomination of invention: Indoor simulation experiment device and method of CO2 flux in intertidal zone with controllable environmental conditions Granted publication date: 20191015 License type: Common License Record date: 20210202 |
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