CN109574237A - A kind of devices, systems, and methods for long term monitoring stain disease comprehensive ecological toxicity up to standard - Google Patents
A kind of devices, systems, and methods for long term monitoring stain disease comprehensive ecological toxicity up to standard Download PDFInfo
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- CN109574237A CN109574237A CN201910045600.2A CN201910045600A CN109574237A CN 109574237 A CN109574237 A CN 109574237A CN 201910045600 A CN201910045600 A CN 201910045600A CN 109574237 A CN109574237 A CN 109574237A
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- 238000012806 monitoring device Methods 0.000 claims abstract description 12
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- XLYOFNOQVPJJNP-ZSJDYOACSA-N Heavy water Chemical compound [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 10
- 239000002002 slurry Substances 0.000 claims description 8
- 241001494246 Daphnia magna Species 0.000 claims description 5
- 241000276569 Oryzias latipes Species 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 239000008399 tap water Substances 0.000 claims description 5
- 235000020679 tap water Nutrition 0.000 claims description 5
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/327—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae characterised by animals and plants
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Abstract
The invention discloses a kind of device, system and method for long term monitoring stain disease comprehensive ecological toxicity up to standard, described device includes water inlet pipe, the cylinder body for constructing Miniature water ecological device, light source, water quality on-line monitoring device, monitoring camera, Toxicity Monitoring net cage, overflow launder and outlet pipe；Water inlet pipe connects the cylinder body, for water to be monitored to be introduced the cylinder body；Light source is arranged above the cylinder body, carries out illumination to water body for simulating daylight；Water quality on-line monitoring device is provided for detecting the water quality parameter of the water body；The inside of the cylinder body is arranged in Toxicity Monitoring net cage, for putting the aquatic animal as target organism in a suitable place to breed；The top of the Toxicity Monitoring net cage is arranged in monitoring camera, for monitoring the existing state of aquatic animal in real time；The top of cylinder body is arranged in overflow launder, and outlet pipe is connected to by the overflow launder with the cylinder body, for draining and keeping the volume substantially constant of the water body.
Description
Technical field
The present invention relates to environmental water quality monitoring technical fields, comprehensive for long term monitoring stain disease up to standard more particularly, to one kind
Close the devices, systems, and methods of eco-toxicity.
Background technique
The fast development of industrialization and urbanization produces a large amount of industry and life stain disease, contains in waste water various types of
Type pollutant, toxic effect merit attention.
Currently, the Toxicity Monitoring of waste water is based on physical and chemical index analysis and Biological Acute Toxicity monitoring, traditional physics and chemistry divides
Analysis can detect the type and concentration of water body Poisoning chemical substance under the limited conditions, but the prior art cannot still identify water-outlet body
The toxic pollutant of middle all kinds；Biological toxicity tests can intuitively reflect the environmental ecology effects of pollutant, but at present only
Based on the Monitoring Acute Toxicity of a small number of aquatiles, and often using based under controlled condition stringent in laboratory
Single known contaminant toxotest method, can not react the poisonous effect of combined pollutant, because being discharged into the dirt of receiving water body
Dye object is possible to that the combined reactions such as collaboration, antagonism, superposition occur in aquatic ecosystem, generates composite toxicity.
It has largely been researched and developed for the Monitoring Acute Toxicity method and monitoring device of the research and development of high concentration toxic pollutant,
Such device or method apply in general to that toxic dangerous material leakage occurs in water body or artificially poisons to wait water pollutions burst
Event, and often there is abnormal movement or mortality in the short time in tested aquatic animal, passes through such monitoring method or device
It can intuitively reflect very much the contamination accident that water quality is subjected to suddenly.It is well known that through sewage treatment plant handle it is up to standard after discharge
All kinds of stain diseases, include low concentration known contaminant and a large amount of types of unknown pollutants etc., low concentration known contaminant in waste water
Chronic toxicity that long-term exposure generates and its still lack with the comprehensive toxicity effect of the compound generation of types of unknown pollutants is probed into, however needle
Pollutant comprehensive ecological toxicity research method and apparatus under this condition are had not been reported.
Therefore, it is necessary to new technologies at least partly to solve limitation existing in the prior art.
Summary of the invention
The present invention is directed to overcome existing water quality biological monitoring method and device not to construct the Ecology of imitation natural conditions
System, can not long term monitoring low concentration pollutant poisonous effect and need to regularly replace biological subject and need for a long time to by
The defects of examination biology feeds food, so that it is comprehensive to propose a kind of new qualified discharge stain disease based on miniature aquatic ecosystem
The method and field device of symphysis state Toxicity Monitoring.
According to an aspect of the present invention, a kind of device for long term monitoring stain disease comprehensive ecological toxicity up to standard is provided,
Include:
Water inlet pipe 4, the cylinder body 6 for constructing Miniature water ecological device, light source 9, water quality on-line monitoring device 11, monitoring are taken the photograph
As head 14, Toxicity Monitoring net cage 15, overflow launder 16 and outlet pipe 17；
Wherein, the water inlet pipe 4 connects the cylinder body 6, for water to be monitored to be introduced the cylinder body 6；The light source 9
It is arranged above the cylinder body 6, illumination is carried out to water body for simulating daylight；The water quality on-line monitoring device 11 is set as using
In the water quality parameter for detecting the water body；The inside of the cylinder body 6 is arranged in the Toxicity Monitoring net cage 15, for putting conduct in a suitable place to breed
The aquatic animal of target organism；The top of the Toxicity Monitoring net cage 15 is arranged in the monitoring camera 14, for supervising in real time
Control the existing state of aquatic animal；The top of cylinder body 6 is arranged in the overflow launder 16, and the outlet pipe 17 passes through the overflow
Slot 16 and be connected to the cylinder body 6, for draining and keeping the volume substantially constant of the water body.
Preferably, described device further includes into water Water-accumulating water cylinder 1, slurry filter 2 and suction pump 3, the water inlet water storage
For storing water to be monitored, the slurry filter 2 is arranged among the water inlet Water-accumulating water cylinder 1 cylinder 1, and is sequentially connected pumping
Water pump 3 and water inlet pipe 4.
Preferably, described device further includes cylinder body frame 7 and lamp bracket 8, and the cylinder body 6 is arranged on the cylinder body frame 7,
The lamp bracket 8 is supported on the cylinder body frame 7, for fixing the light source 9；The monitoring camera 14 is fixed on the lamp bracket
On 8.
Preferably, described device further includes timer 10, for controlling the light application time of the light source 9.
Preferably, described device further includes recirculated water Water-accumulating water cylinder 18 and circulating water pipe 23, the recirculated water Water-accumulating water cylinder 18
The outlet pipe 17 is connected, the water being discharged for accommodating the cylinder body 6；Circulating water pipe 23 connects the water inlet pipe 4, for that will follow
Water circulation in ring water Water-accumulating water cylinder 18 is introduced into the cylinder body 6.
Preferably, described device further includes the circulation water vat drainpipe that 18 side wall upper part of recirculated water Water-accumulating water cylinder is arranged in
19。
Preferably, described device further includes that circulation water vat filter 21 and water circulating pump 22, the water circulating pump 22 are set
It sets among the recirculated water Water-accumulating water cylinder 18, and is sequentially connected and takes out the water circulating pump 22 and the circulating water pipe 23.
Preferably, described device further includes the flow of inlet water regulating valve 5 being arranged on the water inlet pipe 4.
According to another aspect of the present invention, it provides and a kind of is for long term monitoring stain disease comprehensive ecological toxicity up to standard
System, wherein being formed the system comprises device according to the present invention and in the water body of the cylinder body 6 miniature aquatic
State system, the miniature aquatic ecosystem include water plant mud 110, water plant (111,112) and aquatic animal, described aquatic
Animal includes the target organism 113 as instruction comprehensive ecological toxicity.
Preferably, wherein the target organism is selected from zebra fish, medaka, Gobiocypris rarus, crucian and Daphnia magna.
Preferably, wherein the water plant be selected from it is mini to leaf water plant, it is small to leaf water plant, imperial palace grass, hornwort, goat's horn
Crescent moon algae.
Preferably, wherein the aquatic animal further includes selected from least one of river prawn 114 and river snail 115.
Another aspect according to the present invention provides a kind of method for long term monitoring stain disease comprehensive ecological toxicity up to standard,
Include:
Step A: building builds miniature aquatic ecosystem based on water to be monitored in cylinder body 6；
Step B: after a certain time, the toxicity index of the target organism 113 in miniature aquatic ecosystem is detected；
Step C: stain disease comprehensive ecological toxicity up to standard is judged using detected value.
Preferably, wherein step A includes:
It is implanted into water plant mud 110 in cylinder body, introduces certain water to be monitored, then plants heavy water dwarf form water plant kind
Son；
It is shone using 9 simulating sun of artificial light source, so that water plant germination, growing seedling and forming submerged plant monoid；
Emergent aquactic plant is planted on water plant mud 110；And
Dynamic equilibrium of the interior sustained water injection to maintain water inlet to be discharged into cylinder body 6, and it is put into aquatic animal in cylinder body 6,
The aquatic animal includes the target organism 113 as instruction comprehensive ecological toxicity, and the target organism 113 is also placed on cylinder body
In Toxicity Monitoring net cage 15 in 6, miniature aquatic ecosystem is thus constructed.
Preferably, wherein step B includes:
Water quality parameter is detected using water quality on-line monitoring device 11；
The survival for the target organism 113 being arranged in the Toxicity Monitoring net cage 15 is monitored using monitoring camera 14
State；And
Measure the intracorporal toxicity index value of target organism 113.
Preferably, wherein step C includes:
It is compared using the toxicity index value of detection with standard value or the toxicity index value of the detection using experimental group
It is compared with the toxicity index value of control group, thus judges stain disease comprehensive ecological toxicity up to standard, wherein control group and reality
The difference for testing group is to substitute water to be monitored using I or II class water in tap water or water environment quality standard.
Preferably, wherein the target organism 113 is selected from zebra fish, medaka, Gobiocypris rarus, crucian and Daphnia magna.
Preferably, wherein toxicity index superoxide dismutase in the death rate, content of beary metal, musculature
SOD activity, malonaldehyde MDA content in peroxidase POD activity and musculature in musculature.
Preferably, wherein the heavy water dwarf form water plant be selected from it is mini to leaf water plant, it is small to leaf water plant, the emergent aquactic plant
Selected from imperial palace grass, hornwort, goat's horn crescent moon algae；The aquatic animal animal further include in river prawn 114 and river snail 115 at least
One of.
Preferably, wherein the sustained water injection interior into cylinder body 6 with maintain water inlet to be discharged dynamic equilibrium including the use of following
Loop device recycles the water in cylinder body 6.
The present invention simulates nature receiving water body by building Miniature water ecological experiment system, miniature aquatic ecosystem and
Aquatile in Toxicity Monitoring net cage by the sensitive response of pollutant toxicity and toxicant accumulation characteristic come to water body into
Row long term monitoring greatly facilitates the research and analysis of follow-up work personnel, eventually by aforesaid way, can make row up to standard
It puts stain disease to continuously enter in miniature aquatic ecosystem cylinder body, to realize the real-time of qualified discharge stain disease and long-term comprehensive
Symphysis state Toxicity Monitoring.
Detailed description of the invention
Identical appended drawing reference denotes same or similar part or part in attached drawing.Those skilled in the art should manage
Solution, the drawings are not necessarily drawn to scale.Target and feature of the invention will more in view of following description taken together with the accompanying drawings
Add obviously, in attached drawing:
Fig. 1 is the device for long term monitoring stain disease comprehensive ecological toxicity up to standard according to an embodiment of the invention
Schematic front view；
Fig. 2 is the device for long term monitoring stain disease comprehensive ecological toxicity up to standard according to an embodiment of the invention
Schematic side view；
Fig. 3 is the three-dimensional view of miniature aquatic ecosystem cylinder body according to an embodiment of the invention.
Specific embodiment
Clearly to illustrate the scheme in the present invention, preferred embodiment is given below and being described with reference to the accompanying drawings.With
Under explanation be substantially only exemplary and be not intended to limitation the disclosure application or purposes.It should be understood that
In whole attached drawings, corresponding appended drawing reference indicates identical or corresponding component and feature.
Fig. 1 is the device for long term monitoring stain disease comprehensive ecological toxicity up to standard according to an embodiment of the invention
Schematic front view；Fig. 2 is according to an embodiment of the invention for long term monitoring stain disease comprehensive ecological toxicity up to standard
Device schematic side view；Fig. 3 is the three-dimensional view of miniature aquatic ecosystem cylinder body according to an embodiment of the invention.
With reference to Fig. 1-3, as shown, the apparatus may include water inlet Water-accumulating water cylinders 1, slurry filter for the device of the invention
2, suction pump 3, water inlet pipe 4, flow of inlet water regulating valve 5, miniature aquatic ecosystem cylinder body 6, cylinder body frame 7, lamp bracket 8,9 (example of light source
Such as incandescent lamp), timer 10, water quality on-line monitoring device 11, connecting plate 12, camera shooting head gimbal 13, monitoring camera 14, poison
Property monitoring net cage 15, overflow launder 16, outlet pipe 17, recirculated water Water-accumulating water cylinder 18, circulation water vat drainpipe 19, scupper valve 20,
Effluent filter 21, water circulating pump 22, circulating water pipe 23 and circulating water flow adjustable valve 24 are constituted.
As shown, water inlet Water-accumulating water cylinder 1 is for storing water to be monitored, such as the stain disease up to standard of discharge.The sludge
Filter 2 is located at water inlet 1 right side lower end of Water-accumulating water cylinder, and the slurry filter 2 is connect by pipeline with the suction pump 3, described
Suction pump 3 further connect such as screw thread with water inlet pipe 4 and is connected, and the flow of inlet water regulating valve 5 is arranged on water inlet pipe 4, example
It can such as be connected with 4 screw thread of water inlet pipe, the water inlet pipe 4 runs through miniature 6 left side upper end of aquatic ecosystem cylinder body.It in this way can be with
The water in Water-accumulating water cylinder 1 of intaking is introduced into cylinder body 6 by the suction pump 3, wherein slurry filter 2 is for filtering water body
In sludge or big block, prevent blocking suction pump 3, the flow of inlet water regulating valve 5 can according to need to adjust entrance
Water.
The miniature aquatic ecosystem cylinder body 6 is placed on cylinder body frame 7, and the size of the miniature aquatic ecosystem cylinder body 6 can
To be arranged as needed, such as it can be set to long 60cm × wide 30cm × high 35cm, the miniature aquatic ecosystem cylinder
The body lower right corner is equipped with the discharge outlet (not shown) for draining, and the scupper valve is connected with cylinder body screw thread.
The lamp bracket 8 can be located at miniature 6 top of aquatic ecosystem cylinder body, such as the lamp bracket 8 and 7 screw thread phase of cylinder body frame
Even.Lamp bracket 8 is for fixed light source 9 such as incandescent lamp, LED light.These lamp positions in the downside of lamp bracket 8 and with 8 screw thread phase of lamp bracket
Even.Light source such as incandescent lamp is applied not only to grow subsequent water plant germination, is also used to through timer 10 come simulated light
Period simulates normal natural lighting, and thus simulate aquatic system so that present system is consistent with the photoperiod of nature.
The timer 10 is located at 8 upper left side of lamp bracket, such as can be connected through a screw thread to lamp bracket 8, is used for timing.Such as the timing
Device is connected with incandescent lamp driver, for controlling the time switch of incandescent lamp.
The water quality on-line monitoring device 11 can be located at miniature 6 upper left side of aquatic ecosystem cylinder body and can pass through company
Fishplate bar 12 is connected with 6 screw thread of cylinder body.The water quality on-line monitoring device can built-in dissolved oxygen, pH on-line monitoring probe, it is described
Dissolved oxygen, pH on-line monitoring probe etc., for monitoring water quality parameter on-line.Such on-Line Monitor Device is this field
Known to technical staff, do not repeat herein.
The monitoring camera 14 can be connected by camera shooting head gimbal 13 with 8 screw thread of lamp bracket, the Toxicity Monitoring net
Case 15 is located at immediately below monitoring camera 14, and the Toxicity Monitoring net cage 15 can be with miniature 6 screw thread phase of aquatic ecosystem cylinder body
It is even fixed on inside cylinder body 6, the size of the Toxicity Monitoring net cage 15 can according to need to be arranged, such as can be set to
Long 10cm × wide 10cm × high 10cm, such as can have macromolecule splash guard composition, cabinet surrounding is transparent and is equipped with circular hole for example
1mm, the bottom of box can be for example white tight, so that camera is clearly observed and recorded by overlooking.That is, the toxicity is supervised
For placing aquatile in survey grid case 15, it is used for example as the target organism 113 of instruction comprehensive ecological toxicity, monitoring camera 14
It can be able to be 360 degree of high definition rotating cameras with the animation of the on-line real time monitoring biology, the camera.Further,
The settable Light Source Compensation of camera can be used for night observation record.
The overflow launder 16 can be located at 6 upper right side of Miniature water ecological experiment cylinder body, the overflow launder 16 and miniature Ecology
6 screw thread of system cylinder body is connected, and the outlet pipe 17 is connected with 16 screw thread of overflow launder.The outlet pipe 17 described in this way can be by described
Overflow launder 16 and be connected to the cylinder body 6, for draining and keeping the volume substantially constant of the water body.
According to monitoring requirements, assessed if necessary to the long-term comprehensive toxicity to limitation and specific waste discharge, this
The device of invention can also include circulator, be realized waste water to be monitored by circulator in miniature aquatic ecosystem cylinder body
The purpose of 6 internal circulation flows carries out related study on monitoring.And such circulator can intake pump damage, without continuously into
Miniature aquatic ecosystem cylinder body water body self-loopa is realized in the case of water.
More specifically, the device of the invention can also include recirculated water Water-accumulating water cylinder 18.The recirculated water Water-accumulating water cylinder 18 is located at
7 lower end of cylinder body frame, the outlet pipe 17 enters the recirculated water Water-accumulating water cylinder 18 through 7 lower right side of cylinder body frame, thus by cylinder body
The water being discharged in 6 is introduced into recirculated water Water-accumulating water cylinder 18.
The recirculated water Water-accumulating water cylinder 18 can also include the discharge outlet (not shown) that the lower right corner is for example arranged in, circulation water vat
Drainpipe 19 and scupper valve 20.The circulation water vat drainpipe 19 can be located at 18 upper right corner of recirculated water Water-accumulating water cylinder, example
It can such as be connected with 18 screw thread of recirculated water Water-accumulating water cylinder, for the water overflowed in recirculated water Water-accumulating water cylinder 18 to be discharged.The scupper valve
Door 20 is arranged in the draining in 18 lower right corner of recirculated water Water-accumulating water cylinder, such as is connected with 18 screw thread of recirculated water Water-accumulating water cylinder, is used for
The emptying of control loop water Water-accumulating water cylinder 18.
The effluent filter 21 is located at 18 interior lower end of recirculated water Water-accumulating water cylinder, and is connected by pipeline and water circulating pump 22
It connects, the water circulating pump 22 is connected by circulating water pipe 23 with water inlet pipe 4.And recirculated water can be set on circulating water pipe 23
Flow control valve 24, such as the circulating water flow adjustable valve 24 can be connected with 23 screw thread of circulating water pipe.It can pass through in this way
Water in the recirculated water Water-accumulating water cylinder 18 is introduced into cylinder body 6 by the water circulating pump 22, wherein the effluent filter 21 is used
Sludge or solids in filtering water body prevent blocking water circulating pump 22, and the circulating water flow adjustable valve 24 can basis
It needs to adjust the water into cylinder body 6.This circulator can be in the cylinder body 6 and recirculated water Water-accumulating water cylinder 18 as a result,
Between realize circulation.
The method being monitored using apparatus of the present invention is illustrated further below.
It is by water inlet pipe 4 that the test water injection in Water-accumulating water cylinder 1 of intaking is miniature aquatic first by 3 power supply opening of suction pump
Inside state system cylinder body 6, injection water height is about to be laid with centainly apart from cylinder base for example, about 5cm then in cylinder body
The water plant mud 110 (water plant mud is known in the art, therefore is not repeated) of thickness for example, about 5cm thickness, does not have water plant mud just
Cross water body.
Then water plant mud surface uniformly sow it is mini to leaf, it is small to the heavy water dwarf form water plant seed 111 such as leaf water plant, beat
It opens incandescent lamp 9 and timer 10 switchs, according to the time of extraneous actual sunshine length setting timer 10 to control incandescent lamp
Circadian rhythm simulates the effect of day and night.It, can be again to miniature aquatic ecosystem after after water plant germination and growing seedling
Water is slowly injected in cylinder body 6, by adjusting the control of flow of inlet water regulating valve 5 water inlet flow velocity and water, to prevent fiercely impacting heavy water
Water plant can plant imperial palace grass, hornwort, goat's horn crescent moon algae etc. on water plant mud 110 and endure water plant after filling the water certain volume
Object 112 is persistently slowly injected into tested water body by water inlet system, after water plant to be planted is stablized, to miniature aquatic ecosystem cylinder
AB/TU strain wild type adult health zebra fish 113 is launched in body 6, while launching the river prawn 114 and river snail of Suitable Density
115.In addition a small number of AB/TU strain wild type adult health zebra fish 113 will be put into Toxicity Monitoring net cage 15, will be opened again
Real-time monitoring camera 14 records in real time.It should be understood that other than zebra fish is cooked target organism, can also with medaka,
Gobiocypris rarus, crucian, Daphnia magna, etc. aquatic animal in concerned countries standard for wastewater toxicity experiment.
After entire Miniature water ecosystem persistence stable operation, dissolved by the online prison in monitoring water quality on line case 11
The basic water quality parameter of the real-time monitorings qualified discharge stain disease such as oxygen, pH monitoring probe passes through monitoring camera real-time monitoring poison
Property monitoring net cage in zebra fish Burden-Swimming Ability of KM and survival condition.Such as can according to need, by shirtsleeve operation come real
Now to the real-time record of zebra fish motion profile, to judge that water quality condition provides data extremely subsequently through zebra fish action trail
Support.
By regular hour such as two weeks, 1,2 month etc., can monitor in stain disease process-exposed, Miniature water
The death rate of zebra fish in the ecosystem, meanwhile, pass through superoxide dismutase in measurement Heavy Metal Accumulation amount, musculature
(SOD) variation of the immune indexes such as activity, peroxidase (POD) activity, malonaldehyde (MDA) content is to react tested waste water
The detection method of comprehensive ecological toxicity, these substances is well known to those skilled in the art, and is not repeated herein.It can be by these
Detected value is judged compared with relevant criterion or by comparing, that is, identical test macro can be constructed simultaneously,
It changes water inlet into free of contamination aeration tap water, as control, implements comparative monitoring experiment, such as may determine that between two groups
With the presence or absence of significant difference.
Specific embodiment:
Below by taking qualified discharge dyeing waste water as an example, illustrate the specific of comprehensive ecological methods for toxicity monitoring proposed by the present invention
Embodiment.
Using apparatus and method of the present invention, constructs and be for long term monitoring stain disease comprehensive ecological toxicity up to standard
System is carried out qualified discharge dyeing waste water and is monitored to the chronic toxicity of zebra fish in miniature aquatic ecosystem, selects healthy adult male
Property zebra fish (Brachydanio rerio) be test biology, further include in aquatic ecosystem it is mini to leaf water plant, imperial palace grass,
Hornwort, river prawn and river snail.
Zebra fish by zebra fish resource center, Wuhan country provide, a length of 3.4 ± 0.2cm of body, weight be 0.36 ±
0.03g.Experimental fish transports back and temporarily supported 7 days behind laboratory with the tap water of aeration 48h, is then put into zebra fish and builds
In miniature aquatic ecosystem, carry out experiment in 30 days by a definite date, exposed group and control group is respectively set in experiment, and exposure group access is up to standard
Discharge dyeing waste water, control group access aeration tap water, every group of 30 tail zebra fish of dispensing, in statistical experiment group and control in the 30th day
The zebra fish death rate of group, and measure the activity of the SOD in zebra fish muscle tissue, POD activity and MDA content.Referring specifically to, under
Table
Note: a, b indicate that there are significant differences
Experimental data is examined using t, the difference between control group and dyeing waste water exposure group is examined, by table as it can be seen that zebra fish
It is exposed to up-to-standard printing and dying industry wastewater 30 days, SOD activity, POD in comparison control group and the exposure group zebra fish death rate and musculature
Activity and MDA content, research find that the printing and dyeing tail water exposure group zebra fish death rate is higher than control group, and SOD and POD is living in muscle
Property there is significant difference, according to the above results show qualified discharge printing and dyeing tail water to zebra fish have certain chronic toxicity
Effect.
Compared with prior art, a kind of method and dress for long term monitoring stain disease comprehensive ecological toxicity up to standard
It sets, before experiment, water inlet pipe is connected with slurry filter screw thread, is placed in external reservoir, by water inlet pipe and suction pump screw thread
It is connected and runs through miniature aquatic ecosystem cylinder body, then intake pump is connected with external power supply, staff starts intake pump, makes
Water inlet pipe is in unimpeded state, is obstructed by slurry filter to the silt in qualified discharge waste water, makes clear water body
It is extracted into miniature aquatic ecosystem cylinder body pond, raw water is injected inside miniature aquatic ecosystem by water inlet pipe, this system is not pacified
Dress heating or refrigeration equipment, purpose are just intended to scene and reproduce the true receiving aquatic ecosystem after qualified discharge stain disease outlet,
The sunshine situation and the rhythm and pace of moving things for passing through incandescent lamp and the timer models four seasons again, simulate sunshine length and the day and night in each season
Effect can be by adjusting flow and the control water inlet of leading water of water pump, to control when being discharged water by water pipe into water vat
The mobility status of water in water vat, simulates the water flow of true rivers and lakes, improves the adaptation of aquatile in miniature aquatic ecosystem
Ability passes through the base of online prison dissolved oxygen, pH monitoring probe real-time monitoring qualified discharge stain disease in monitoring water quality on line case
This water quality parameter can obtain the growth condition of water quality of aquatile in miniature aquatic ecosystem by continuously monitoring, and be subsequent
It launches and data supporting is provided, the experiment fish in Toxicity Monitoring net cage can be shot by monitoring camera, in the present invention
Cylinder body and partition material are ultra-clear glasses, not only convenient for cleaning, are provided simultaneously with the good transparency, are convenient for camera and work
Personnel clearly observe and record, and facilitate researching and analysing for subsequent personnel.
Meanwhile when the water level of miniature aquatic ecosystem cylinder body reaches spilling water line, water can enter spilling water by overflow launder
Pipe, and then recirculated water Water-accumulating water cylinder is flowed by outlet pipe, recirculated water Water-accumulating water cylinder is also equipped with overflow launder and drainpipe, is directly connected to
Sewer, caring for the water body being discharged from miniature aquatic ecosystem can be by the direct outlet of recirculated water Water-accumulating water cylinder or by water circulating pump
It refills miniature aquatic ecosystem cylinder body and carries out self-loopa, simulate the stronger river receiving water body of mobility and opposite stream respectively
The receiving water bodies such as the dynamic lesser pond of property such as simulate the more slow river of flow velocity, and flow velocity can be about 20-30ml/min.
When needing to carry out cleaning-sterilizing or cleaning to miniature aquatic ecosystem or recirculated water Water-accumulating water cylinder, cylinder body need to be only opened
The intracorporal water discharge of cylinder can be carried out subsequent operation by the scupper valve in the lower right corner, which is used for long term monitoring dirt up to standard
The method and apparatus of waste water comprehensive ecological toxicity, ingenious in design, method is accurate, powerful, is popped one's head in by monitoring water quality on line
Real time monitoring enter system qualified discharge stain disease water quality condition, it can be achieved that water quality situation to be monitored preliminary judgement.
By constructing Miniature water ecological experiment system, nature receiving water body is simulated, in miniature aquatic ecosystem and Toxicity Monitoring net cage
Aquatile by pollutant toxicity it is sensitive response and toxicant accumulation characteristic come to water body carry out long term monitoring, greatly
The research and analysis for facilitating follow-up work personnel, eventually by aforesaid way, can make qualified discharge stain disease continuously into
Enter into miniature aquatic ecosystem cylinder body, to realize the real-time and long-term comprehensive ecological toxicity prison of qualified discharge stain disease
It surveys.
Used herein a specific example illustrates the principle and implementation of the invention, and above embodiments are said
It is bright to be merely used to help understand the device of the invention and its core concept；At the same time, for those skilled in the art, foundation
Thought of the invention, there will be changes in the specific implementation manner and application range, in conclusion the content of the present specification is not
It is interpreted as limitation of the present invention.
Claims (10)
1. a kind of device for long term monitoring stain disease comprehensive ecological toxicity up to standard characterized by comprising
Water inlet pipe (4), the cylinder body (6) for constructing Miniature water ecological device, light source (9), water quality on-line monitoring device (11), prison
Control camera (14), Toxicity Monitoring net cage (15), overflow launder (16) and outlet pipe (17)；
Wherein, the water inlet pipe (4) connects the cylinder body (6), for water to be monitored to be introduced the cylinder body (6)；The light
Source (9) setting carries out illumination to water body above the cylinder body (6), for simulating daylight；The water quality on-line monitoring device
(11) it is provided for detecting the water quality parameter of the water body；The Toxicity Monitoring net cage (15) is arranged in the cylinder body (6)
Inside, for putting the aquatic animal as target organism in a suitable place to breed；The monitoring camera (14) is arranged in the Toxicity Monitoring net cage
(15) top, for monitoring the existing state of aquatic animal in real time；The overflow launder (16) is arranged on the top of cylinder body (6),
The outlet pipe (17) is connected to by the overflow launder (16) with the cylinder body (6), for draining and keeping the water body
Volume substantially constant.
2. the apparatus according to claim 1, which is characterized in that described device further includes into water Water-accumulating water cylinder (1), sludge filtration
Device (2) and suction pump (3), the water inlet Water-accumulating water cylinder (1) is for storing water to be monitored, slurry filter (2) setting
Among the water inlet Water-accumulating water cylinder (1), and it is sequentially connected suction pump (3) and water inlet pipe (4).
3. the apparatus according to claim 1, which is characterized in that described device further includes cylinder body frame (7) and lamp bracket (8),
The cylinder body (6) is arranged on the cylinder body frame (7), and the lamp bracket (8) is supported on the cylinder body frame (7), for fixing
State light source (9)；The monitoring camera (14) is fixed on the lamp bracket (8).
4. a kind of system for long term monitoring stain disease comprehensive ecological toxicity up to standard, which is characterized in that the system comprises roots
The miniature aquatic ecosystem formed according to device of any of claims 1-3 and in the water body of the cylinder body (6),
The miniature aquatic ecosystem includes water plant mud (110), water plant (111,112) and aquatic animal, the aquatic animal
Target organism (113) including being used as instruction comprehensive ecological toxicity.
5. system according to claim 4, wherein the target organism is selected from zebra fish, medaka, Gobiocypris rarus, crucian
And Daphnia magna.
6. a kind of method for long term monitoring stain disease comprehensive ecological toxicity up to standard, comprising:
Step A: building builds miniature aquatic ecosystem based on water to be monitored in cylinder body (6)；
Step B: after a certain time, the toxicity index of the target organism (113) in miniature aquatic ecosystem is detected；
Step C: stain disease comprehensive ecological toxicity up to standard is judged using detected value.
7. according to the method described in claim 6, wherein step A includes:
Water plant mud (110) are implanted into cylinder body, introduce certain water to be monitored, then plant heavy water dwarf form water plant seed；
It is shone using artificial light source (9) simulating sun, so that water plant germination, growing seedling and forming submerged plant monoid；
Emergent aquactic plant is planted on water plant mud 110；And
Dynamic equilibrium of the interior sustained water injection to maintain water inlet to be discharged into cylinder body 6, and it is put into aquatic animal in cylinder body 6, it is described
Aquatic animal includes the target organism (113) as instruction comprehensive ecological toxicity, and the target organism (113) is also placed on cylinder body
In Toxicity Monitoring net cage (15) in 6, miniature aquatic ecosystem is thus constructed.
8. according to the method described in claim 6, wherein step B includes:
Water quality parameter is detected using water quality on-line monitoring device (11)；
It deposits the target organism (113) being arranged in the Toxicity Monitoring net cage (15) using monitoring camera (14) monitoring
State living；And
Measure target organism (113) intracorporal toxicity index value.
9. according to the method described in claim 6, wherein step (C) includes:
Be compared using the toxicity index value of detection with standard value or using experimental group detection toxicity index value with it is right
It is compared according to the toxicity index value of group, thus judges stain disease comprehensive ecological toxicity up to standard, wherein control group and experimental group
Difference be to substitute water to be monitored using I or II class water in tap water or water environment quality standard.
10. according to the method described in claim 6, wherein the target organism (113) is selected from zebra fish, medaka, dilute has Minnow
Crucian carp, crucian and Daphnia magna.
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| CN110208475A (en) * | 2019-05-27 | 2019-09-06 | 生态环境部南京环境科学研究所 | Water environmental factor based on Daphnia magna toxicity monitors experimental provision |
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| CN109574237B (en) | 2024-03-22 |
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