CN110208475A - Water environmental factor based on Daphnia magna toxicity monitors experimental provision - Google Patents
Water environmental factor based on Daphnia magna toxicity monitors experimental provision Download PDFInfo
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- CN110208475A CN110208475A CN201910448149.9A CN201910448149A CN110208475A CN 110208475 A CN110208475 A CN 110208475A CN 201910448149 A CN201910448149 A CN 201910448149A CN 110208475 A CN110208475 A CN 110208475A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 178
- 241001494246 Daphnia magna Species 0.000 title claims abstract description 83
- 230000007613 environmental effect Effects 0.000 title claims abstract description 33
- 231100000419 toxicity Toxicity 0.000 title claims abstract description 26
- 230000001988 toxicity Effects 0.000 title claims abstract description 26
- 238000012544 monitoring process Methods 0.000 claims abstract description 42
- 238000003860 storage Methods 0.000 claims abstract description 40
- 239000002699 waste material Substances 0.000 claims abstract description 16
- 239000006184 cosolvent Substances 0.000 claims description 34
- 238000002474 experimental method Methods 0.000 claims description 17
- 238000009304 pastoral farming Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 15
- 238000005286 illumination Methods 0.000 claims description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 230000002572 peristaltic effect Effects 0.000 claims description 10
- 238000012549 training Methods 0.000 claims description 9
- 229920000742 Cotton Polymers 0.000 claims description 5
- 238000007667 floating Methods 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 238000003760 magnetic stirring Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 230000010412 perfusion Effects 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 238000011144 upstream manufacturing Methods 0.000 claims description 2
- 230000004888 barrier function Effects 0.000 claims 1
- 238000013461 design Methods 0.000 abstract description 3
- 238000004088 simulation Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 78
- 238000012360 testing method Methods 0.000 description 15
- 239000000126 substance Substances 0.000 description 12
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 230000007665 chronic toxicity Effects 0.000 description 4
- 231100000160 chronic toxicity Toxicity 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000007774 longterm Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 241000238571 Cladocera Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 230000002452 interceptive effect Effects 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000003507 refrigerant Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 206010001488 Aggression Diseases 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 241000238423 Branchiopoda Species 0.000 description 1
- 241000243686 Eisenia fetida Species 0.000 description 1
- 241000593884 Leptostraca Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 231100000567 intoxicating Toxicity 0.000 description 1
- 230000002673 intoxicating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000006303 photolysis reaction Methods 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000133 toxic exposure Toxicity 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
A kind of water environmental factor monitoring experimental provision based on Daphnia magna toxicity, comprising: Daphnia magna culture vessel (1), environment culture solution deployment unit (2), the storage of environment culture solution are with unit (3), the supply dispatching of environment culture solution unit (4), waste disposal unit (5)；With being connected respectively by environment culture solution supply dispatching unit (4) between unit (3) and environment culture solution supply dispatching unit (4), the Daphnia magna culture vessel (1) is connected with waste disposal unit (5) for the Daphnia magna culture vessel (1), environment culture solution deployment unit (2), the storage of environment culture solution；The device of the invention structure is simple, design rationally, can accurate simulation based on Daphnia magna toxicity water environmental factor monitor, provide reliable foundation for experimental study.
Description
Technical field
The present invention relates to environmental water quality monitoring technical fields, and in particular, to a kind of water body ring based on Daphnia magna toxicity
The border factor monitors experimental provision.
Background technique
The fast development of industrialization and urbanization produces a large amount of industry and life stain disease, contains in waste water various types of
Type pollutant, toxic effect merit attention.
Daphnia magna is also referred to as water flea or scratches, and is commonly called as red worm or worm for fishing, belongs to cladocera (Cladocera), refer to Arthropoda,
Crustachia, Branchiopoda, double Leptostracas, branch angle suborder animal.Daphnia magna has as the one mode biology of environmental pollution monitoring
There is great scientific research utility value, there is weight in terms of the test of pesticide, the acute toxicity and chronic toxicity of chemicals and environmental hormone effects
The effect wanted.
The Daphnia magna toxicity testing standard of blocking has been formed in currently available technology.In order to guarantee the science of experiment
Property, the comparativity of validity and experimental result, test of the most confirmation request tested substance of these methods in entire test period
It can keep relative stability in system.If tested substance is relatively stable, toxic exposure experiment does not update within entire test period
Solution is carried out；It otherwise must be using the experimental method for regularly updating solution.Since maximum solution renewal frequency can only generally reach
It is necessary if its degradation half life is less than for 24 hours for the unstable tested substance of facile hydrolysis, volatile, easy photodissociation etc. to for 24 hours
By other way.This unstable tested substance belongs to " test difficult substances ", accounts among industrial chemistry substance and pesticide
Quite a few of body, an important factor for being the main bugbear of Ecology toxotest and influence test accuracy.
In addition, the chronic toxicities such as breeding, development and environment incretion interfering intoxicating period are generally longer, must cover
Biological major part life cycle or Life cycle.In order to reduce because tested substance degradation or loss to test bring deviation,
Study chemical substance chronic toxicity, the interfering Ecology toxotest of environment incretion it must also be ensured that test solution longer
Biological subject is exposed with more stable concentration in the toxotest period.
Due to above-mentioned many complicated, fine, special technical requirements, not yet occurs satisfactory equipment currently on the market
Or device, existing experimental method not only low efficiency, but also be difficult to cope with the testing requirement and change of unstability " difficult substances "
Substance chronic toxicity, the interfering Ecology toxicity of environment incretion is learned to study for a long period of time demand.This is tested chemical substance and section
It learns research and brings larger difficult and error.
Summary of the invention
To solve the above problems, the present invention provides a kind of, the water environmental factor monitoring experiment based on Daphnia magna toxicity is filled
It sets.The device of the invention structure is simple, and design rationally, being capable of water environmental factor prison of the accurate simulation based on Daphnia magna toxicity
It surveys, provides reliable foundation for experimental study.
Used technical solution is a kind of water body ring based on Daphnia magna toxicity to the present invention to solve above-mentioned technical problem
The border factor monitors experimental provision, comprising: Daphnia magna culture vessel, environment culture solution deployment unit, the storage of environment culture solution are with single
Member, environment culture solution supply dispatching unit, waste disposal unit；The Daphnia magna culture vessel, environment culture solution deployment unit,
The storage of environment culture solution is with single by the supply dispatching of environment culture solution respectively between unit and environment culture solution supply dispatching unit
Member is connected, and the Daphnia magna culture vessel is connected with waste disposal unit；
The Daphnia magna culture vessel include water inlet pipe, container body, lighting apparatus, water environmental factor monitoring unit,
Monitoring unit, water bath device and outlet pipe；
Experiment emulated ecological environment is formed in the water body environment of container body, the experiment emulated ecological environment includes becoming silted up
Mud, water plant and Daphnia magna；Baffling device is set in container body, baffling device by container body be separated into cultivation region and
The both ends of connection rectangle circulation canal, the upstream end of rectangle circulation canal fluid are distinguished in the part of race way two, race way both ends
Equipped with water pump, downstream is equipped with collection device, and water pump and collection device are arranged in rectangle circulation canal；It is set on baffling device
There is the through-hole of several funnel structures, wide opening one end of through-hole is located at cultivation region side, and slot one end of through-hole is followed positioned at rectangle
Ring channel side, baffling device are transparent configuration, around the slot of through-hole on be also provided with other lighting apparatus.
Preferably, the environment culture solution deployment unit includes automatic liquid dispenser, cosolvent device, cosolvent control preparation
Device, environment culture solution device for formulating, the cosolvent device, cosolvent compare device for formulating, environment culture solution device for formulating
It is respectively placed on independent magnetic stirring apparatus；
In any of the above-described scheme preferably, the environment culture solution storage includes the temporary dress of cosolvent control with unit
Set, environment culture solution apparatus for temporary storage, above-mentioned cosolvent control apparatus for temporary storage, environment culture solution apparatus for temporary storage entrance respectively with help
The outlet of solvent control device for formulating, environment culture solution device for formulating is connected by pipeline, and pipeline is equipped with solenoid valve；It is all temporary
Cryopreservation device is brown glass material, and is equipped with top cover, and top cover apertured is inserted into for the perfusion tube of peristaltic pump.
In any of the above-described scheme preferably, environment culture solution supply dispatching unit include liquid level sensor, from
Moving electromagnetic valve, peristaltic pump and PLC controller block water flow or connection water flow by PLC controller automatically.
In any of the above-described scheme preferably, the waste disposal unit include Spaced setting filter cotton layer and
Activated carbon filter layer, the two are integrated in closed cell, only pass through the outlet pipe phase of water inlet pipe and the Daphnia magna culture vessel
Even.
In any of the above-described scheme preferably, the water inlet pipe connects the container body, for by water to be monitored
Introduce the container body；The lighting apparatus is arranged above the container body, carries out light to water body for simulating daylight
According to；The water environmental factor monitoring unit is provided for detecting the water quality parameter of the water body；The monitoring unit setting
In the top of the container body, for monitoring the existing state of Daphnia magna in real time；The outlet pipe and the container body connect
It is logical, for draining and keeping the volume substantially constant of the water body.
In any of the above-described scheme preferably, launching in the cultivation region has enclosed grazing device, the enclosed grazing
Device includes package grid, is enclosed with feed and floating ball in the package grid, is provided with fixation on the enclosed grazing device
Rope, the fixed rope are posted above the container body.
In any of the above-described scheme preferably, the Daphnia magna culture vessel further includes support frame and lamp bracket, described
Container body is arranged on support frame as described above, and the lamp bracket is supported on support frame as described above, for fixing the lighting apparatus；Institute
It states monitoring unit to be fixed on the lamp bracket, the water bath device is arranged below the container body；Further include timer, uses
In the light application time for controlling the lighting apparatus.
The present invention be according to the practical application of many years practice and experience obtained by, using optimal technological means and measure come into
Row Combinatorial Optimization, obtains optimal technical effect, is not the simple superposition of technical characteristic and pieces together, therefore the present invention has
Significant meaning.
Beneficial effects of the present invention:
1. the present invention simulates nature receiving water body, the Daphnia magna tested in emulated ecological environment passes through to pollutant toxicity
Sensitivity response and toxicant accumulation characteristic to carry out long term monitoring to water body, greatly facilitate the research of follow-up work personnel
Qualified discharge stain disease can be made to continuously enter the container of experiment emulated ecological environment eventually by aforesaid way with analysis
In main body, to realize the real-time and long-term comprehensive ecological Toxicity Monitoring of qualified discharge stain disease.
2. the present invention realizes preparing and automatically update and feed automatically for certain concentration environment culture solution；It realizes large-scale
Magna and environment culture solution come into full contact with and complete the life processes such as development, breeding, at the same avoid the escape of female Magna and children Magna and
Mechanical injuries.
3. the present invention realizes the processing such as filtering, the absorption of test waste water, environmental hazard is reduced；Experimental provision is convenient for test
It operates, is at low cost, being easy to laboratory popularization.
Brief Description Of Drawings
Fig. 1 is the schematic diagram of the water environmental factor monitoring experimental provision according to the present invention based on Daphnia magna toxicity；
Fig. 2 is the Daphnia magna training of the water environmental factor monitoring experimental provision according to the present invention based on Daphnia magna toxicity
Support the schematic diagram of container.
Specific embodiment
Below in conjunction with the drawings and specific embodiments, the invention will be further described, but claimed range is not limited to
In this.
Embodiment 1
Referring to Fig. 1-2, a kind of water environmental factor monitoring experimental provision based on Daphnia magna toxicity, comprising: Daphnia magna training
Feeding container 1, environment culture solution deployment unit 2, the storage of environment culture solution dispense unit 4 with unit 3, the supply of environment culture solution, give up
Liquid processing unit 5；Unit 3 and ring are matched in the Daphnia magna culture vessel 1, environment culture solution deployment unit 2, the storage of environment culture solution
It is connected respectively by environment culture solution supply dispatching unit 4 between border culture solution supply dispatching unit 4, the Daphnia magna culture is held
Device 1 is connected with waste disposal unit 5；
The Daphnia magna culture vessel 1 includes water inlet pipe, container body 6, lighting apparatus 7, water environmental factor monitoring list
Member 8, monitoring unit 9, water bath device 10 and outlet pipe；
Experiment emulated ecological environment is formed in the water body environment of container body 6, the experiment emulated ecological environment includes becoming silted up
Mud, water plant and Daphnia magna；Baffling device 11 is set in container body 6, and container body 6 is separated into culture by baffling device
Area and the part of race way two, race way both ends distinguish connection rectangle circulation canal both ends, rectangle circulation canal fluid it is upper
It swims end and is equipped with water pump, downstream is equipped with collection device, and water pump and collection device are arranged in rectangle circulation canal；Baffling device
11 are equipped with the through-hole of several funnel structures, and wide opening one end of through-hole is located at cultivation region side, and slot one end of through-hole is located at
Rectangle circulation canal side, baffling device 11 are transparent configuration, around the slot of through-hole on be also provided with other illumination and set
It is standby.
The environment culture solution deployment unit 2 include automatic liquid dispenser, cosolvent device, cosolvent control device for formulating,
Environment culture solution device for formulating, the cosolvent device, cosolvent control device for formulating, environment culture solution device for formulating are set respectively
In on independent magnetic stirring apparatus；
The environment culture solution storage includes that cosolvent compares apparatus for temporary storage, environment culture solution apparatus for temporary storage with unit 3, on
State cosolvent control apparatus for temporary storage, the entrance of environment culture solution apparatus for temporary storage compares device for formulating, environment training with cosolvent respectively
The outlet of nutrient solution device for formulating is connected by pipeline, and pipeline is equipped with solenoid valve；All apparatus for temporary storage are brown glass material,
And it is equipped with top cover, top cover apertured is inserted into for the perfusion tube of peristaltic pump.
The environment culture solution supply dispatching unit 4 includes liquid level sensor, automatic electromagnetic valve, peristaltic pump and PLC control
Device blocks water flow or connection water flow by PLC controller automatically.
The waste disposal unit includes that the filter cotton layer of Spaced setting and activated carbon filter layer, the two are integrated in envelope
It closes in unit, is only connected by water inlet pipe with the outlet pipe of the Daphnia magna culture vessel 1.
The water inlet pipe connects the container body, for water to be monitored to be introduced the container body 6；The illumination
Equipment 7 is arranged above the container body 6, carries out illumination to water body for simulating daylight；The water environmental factor monitoring
Unit 8 is provided for detecting the water quality parameter of the water body；The top of the container body 6 is arranged in the monitoring unit 9,
For monitoring the existing state of Daphnia magna in real time；The outlet pipe is connected to the container body 6, described for draining and keeping
The volume substantially constant of water body.
Launching in the cultivation region has enclosed grazing device, and the enclosed grazing device includes package grid, the package
It is enclosed with feed and floating ball in grid, fixed rope is provided on the enclosed grazing device, the fixed rope is posted in the container
6 top of main body.
The Daphnia magna culture vessel 1 further includes support frame and lamp bracket, and the container body 6 is arranged in support frame as described above
On, the lamp bracket is supported on support frame as described above, for fixing the lighting apparatus 7；The monitoring unit 9 is fixed on the lamp
On frame, the water bath device 10 is arranged below the container body 6；It further include timer, for controlling the lighting apparatus 7
Light application time.
Baffling device 11 is transparent configuration, around the slot of through-hole on be also provided with other lighting apparatus, by solid
After fixed rope takes out enclosed grazing device, then opening other lighting apparatus makes it shine, and Daphnia magna is due to its phototaxis at this time,
It can be to enrichment of swimming at baffling device 11.
Embodiment 2
Referring to Fig. 1-2, a kind of water environmental factor monitoring experimental provision based on Daphnia magna toxicity, comprising: Daphnia magna training
Feeding container 1, environment culture solution deployment unit 2, the storage of environment culture solution dispense unit 4 with unit 3, the supply of environment culture solution, give up
Liquid processing unit 5；Unit 3 and ring are matched in the Daphnia magna culture vessel 1, environment culture solution deployment unit 2, the storage of environment culture solution
It is connected respectively by environment culture solution supply dispatching unit 4 between border culture solution supply dispatching unit 4, the Daphnia magna culture is held
Device 1 is connected with waste disposal unit 5；
The Daphnia magna culture vessel 1 includes water inlet pipe, container body 6, lighting apparatus 7, water environmental factor monitoring list
Member 8, monitoring unit 9, water bath device 10 and outlet pipe；
Experiment emulated ecological environment is formed in the water body environment of container body 6, the experiment emulated ecological environment includes becoming silted up
Mud, water plant and Daphnia magna；Baffling device 11 is set in container body 6, and container body 6 is separated into culture by baffling device
Area and the part of race way two, race way both ends distinguish connection rectangle circulation canal both ends, rectangle circulation canal fluid it is upper
It swims end and is equipped with water pump, downstream is equipped with collection device, and water pump and collection device are arranged in rectangle circulation canal；Baffling device
11 are equipped with the through-hole of several funnel structures, and wide opening one end of through-hole is located at cultivation region side, and slot one end of through-hole is located at
Rectangle circulation canal side, baffling device 11 are transparent configuration, around the slot of through-hole on be also provided with other illumination and set
It is standby.
The environment culture solution deployment unit 2 include automatic liquid dispenser, cosolvent device, cosolvent control device for formulating,
Environment culture solution device for formulating, the cosolvent device, cosolvent control device for formulating, environment culture solution device for formulating are set respectively
In on independent magnetic stirring apparatus；
The environment culture solution storage includes that cosolvent compares apparatus for temporary storage, environment culture solution apparatus for temporary storage with unit 3, on
State cosolvent control apparatus for temporary storage, the entrance of environment culture solution apparatus for temporary storage compares device for formulating, environment training with cosolvent respectively
The outlet of nutrient solution device for formulating is connected by pipeline, and pipeline is equipped with solenoid valve；All apparatus for temporary storage are brown glass material,
And it is equipped with top cover, top cover apertured is inserted into for the perfusion tube of peristaltic pump.
The environment culture solution supply dispatching unit 4 includes liquid level sensor, automatic electromagnetic valve, peristaltic pump and PLC control
Device blocks water flow or connection water flow by PLC controller automatically.
The waste disposal unit includes that the filter cotton layer of Spaced setting and activated carbon filter layer, the two are integrated in envelope
It closes in unit, is only connected by water inlet pipe with the outlet pipe of the Daphnia magna culture vessel 1.
The water inlet pipe connects the container body, for water to be monitored to be introduced the container body 6；The illumination
Equipment 7 is arranged above the container body 6, carries out illumination to water body for simulating daylight；The water environmental factor monitoring
Unit 8 is provided for detecting the water quality parameter of the water body；The top of the container body 6 is arranged in the monitoring unit 9,
For monitoring the existing state of Daphnia magna in real time；The outlet pipe is connected to the container body 6, described for draining and keeping
The volume substantially constant of water body.
Launching in the cultivation region has enclosed grazing device, and the enclosed grazing device includes package grid, the package
It is enclosed with feed and floating ball in grid, fixed rope is provided on the enclosed grazing device, the fixed rope is posted in the container
6 top of main body.
The Daphnia magna culture vessel 1 further includes support frame and lamp bracket, and the container body 6 is arranged in support frame as described above
On, the lamp bracket is supported on support frame as described above, for fixing the lighting apparatus 7；The monitoring unit 9 is fixed on the lamp
On frame, the water bath device 10 is arranged below the container body 6；It further include timer, for controlling the lighting apparatus 7
Light application time.
In addition, water bath device 10 includes support frame, a water bath is placed in support on support frame, and water bath is equipped with water-bath
Slot water inlet, safe gap and water bath water return outlet, water bath water inlet and water bath water return outlet are equipped with control valve.
Water bath and water tank form circulation loop, are equipped at least one heat-exchange tube in water tank, one end of heat-exchange tube is set
There are Heating medium and/or refrigerant inlet, the other end of heat-exchange tube is correspondingly provided with heating agent outlet and/or refrigerant exit,
The arrival end of heat-exchange tube is equipped with temperature and controls to adjust valve, controls to adjust valve regulation heating agent or cold medium flux, raising by temperature
System 6 controls to adjust valve 25 by temperature and switches internal circulation system and external circulating system.
The water bath water inlet of constant temperature water bath is connected to water outlet of water tank, in safe gap and water bath water return outlet
Water be back to water tank.The arrival end of water bath or outlet end are equipped with temperature-sensing probe, and temperature-sensing probe transmits detection data
To remote controllers, remote controllers control temperature according to detection data and control to adjust valve.The water outlet of water tank is equipped with water pump
One, water pump two is equipped between the water return outlet of water tank.
Water supplement port, gap, observation maintenance window, water level indicator tube and water tank cleaning discharge outlet, water level are additionally provided on water tank
Pipe is indicated to be set to the transparent pipe that water tank is outer and is connected to water tank bottom.It is wrapped up in outside water tank, water bath and each pipeline of connection
It is covered with the thermal insulation material for heat preservation.At least one temp probe is distributed in water tank and in water bath.
The water bath device 10 can efficiently utilize the limited space in laboratory, realize centralized management, save test at
This.It is controlled to adjust by the heat-exchange tube being arranged in water tank, and in the arrival end of refrigerant inlet and Heating medium equipped with temperature
Valve controls to adjust Vavle switching heating circulation system and down cycles system by temperature.Temperature is equipped in the arrival end of heat-exchange tube
Degree controls to adjust valve, so that the water constant for meeting water temperature requirement flows in series through water bath device, realizes and maintains the constant water temperature of sink
Target, be remarkably improved energy utilization efficiency, while being conducive to Water Factors precise constant, water temperature fluctuation amplitude be less than ±
0.5 DEG C, salinity altercation is minimum, and for other water quality indicators without significant change, facility cost is lower, and maintenance is simple.
Embodiment 3
Referring to Fig. 1-2, a kind of water environmental factor monitoring experimental provision based on Daphnia magna toxicity, comprising: Daphnia magna training
Feeding container 1, environment culture solution deployment unit 2, the storage of environment culture solution dispense unit 4 with unit 3, the supply of environment culture solution, give up
Liquid processing unit 5；Unit 3 and ring are matched in the Daphnia magna culture vessel 1, environment culture solution deployment unit 2, the storage of environment culture solution
It is connected respectively by environment culture solution supply dispatching unit 4 between border culture solution supply dispatching unit 4, the Daphnia magna culture is held
Device 1 is connected with waste disposal unit 5；
The Daphnia magna culture vessel 1 includes water inlet pipe, container body 6, lighting apparatus 7, water environmental factor monitoring list
Member 8, monitoring unit 9, water bath device 10 and outlet pipe；
Experiment emulated ecological environment is formed in the water body environment of container body 6, the experiment emulated ecological environment includes becoming silted up
Mud, water plant and Daphnia magna；Baffling device 11 is set in container body 6, and container body 6 is separated into culture by baffling device
Area and the part of race way two, race way both ends distinguish connection rectangle circulation canal both ends, rectangle circulation canal fluid it is upper
It swims end and is equipped with water pump, downstream is equipped with collection device, and water pump and collection device are arranged in rectangle circulation canal；Baffling device
11 are equipped with the through-hole of several funnel structures, and wide opening one end of through-hole is located at cultivation region side, and slot one end of through-hole is located at
Rectangle circulation canal side, baffling device 11 are transparent configuration, around the slot of through-hole on be also provided with other illumination and set
It is standby.
The environment culture solution deployment unit 2 include automatic liquid dispenser, cosolvent device, cosolvent control device for formulating,
Environment culture solution device for formulating, the cosolvent device, cosolvent control device for formulating, environment culture solution device for formulating are set respectively
In on independent magnetic stirring apparatus；
The environment culture solution storage includes that cosolvent compares apparatus for temporary storage, environment culture solution apparatus for temporary storage with unit 3, on
State cosolvent control apparatus for temporary storage, the entrance of environment culture solution apparatus for temporary storage compares device for formulating, environment training with cosolvent respectively
The outlet of nutrient solution device for formulating is connected by pipeline, and pipeline is equipped with solenoid valve；All apparatus for temporary storage are brown glass material,
And it is equipped with top cover, top cover apertured is inserted into for the perfusion tube of peristaltic pump.
The environment culture solution supply dispatching unit 4 includes liquid level sensor, automatic electromagnetic valve, peristaltic pump and PLC control
Device blocks water flow or connection water flow by PLC controller automatically.
The waste disposal unit includes that the filter cotton layer of Spaced setting and activated carbon filter layer, the two are integrated in envelope
It closes in unit, is only connected by water inlet pipe with the outlet pipe of the Daphnia magna culture vessel 1.
The water inlet pipe connects the container body, for water to be monitored to be introduced the container body 6；The illumination
Equipment 7 is arranged above the container body 6, carries out illumination to water body for simulating daylight；The water environmental factor monitoring
Unit 8 is provided for detecting the water quality parameter of the water body；The top of the container body 6 is arranged in the monitoring unit 9,
For monitoring the existing state of Daphnia magna in real time；The outlet pipe is connected to the container body 6, described for draining and keeping
The volume substantially constant of water body.
Launching in the cultivation region has enclosed grazing device, and the enclosed grazing device includes package grid, the package
It is enclosed with feed and floating ball in grid, fixed rope is provided on the enclosed grazing device, the fixed rope is posted in the container
6 top of main body.
The Daphnia magna culture vessel 1 further includes support frame and lamp bracket, and the container body 6 is arranged in support frame as described above
On, the lamp bracket is supported on support frame as described above, for fixing the lighting apparatus 7；The monitoring unit 9 is fixed on the lamp
On frame, the water bath device 10 is arranged below the container body 6；It further include timer, for controlling the lighting apparatus 7
Light application time.
In addition, in the cultivation region of the container body 6 also settable mother Magna and children Magna separator, including bolter
Net, valve and pipeline.Separating screen includes the upper bolter for engaging setting with the sealing of the inner wall of container body 6 respectively from top to bottom
Net and lower separating screen.The nylon screen that upper separating screen is 1100 μm, the nylon screen that lower separating screen is 550 μm, this 2
Fineness is to can guarantee the accuracy of separation by testing the optimal separation fineness obtained repeatedly.Valve setting connects with water outlet
On logical pipeline.The separator can be made the various different shapes such as circle, quadrangle, and two separating screens are by agreeing with
Bayonet realized with the container body 6 it is closed.The liquid separated in cylinder is bled off step by step as needed by control valve, it will
Female Magna and children Magna, which is quickly trapped in respectively on the separating screen of different fineness, realizes separation.
The separator and selects optimal fineness using two and the separating screen for separating cylinder and engaging sealed set, from
And can separate quick, convenient to Daphnia magna mother Magna and children Magna realization, cleanly, convenient for directly making in laboratory or scene
With.
The separator structure is simple, operates also very convenient, suitable small-scale and experimental occasions；It is ingenious in design, it is easy to real
It applies, is worthy to be popularized.
In addition, to realize more preferably technical effect, it can also be by technical solution any combination in above-described embodiment, to meet
The demand of various practical applications.
In the above examples 1-3, starting the PLC program in PLC controller, system starts to prepare environment culture solution,
Environment culture solution will store after the completion of preparing automatically into environment culture solution matches unit 3, is then automatically continuously pumped into Daphnia magna training
It supports in container 1, the environment culture solution beyond 1 capacity of Daphnia magna culture vessel is constantly discharged to waste disposal unit from water outlet,
Waste disposal unit is filtered, will be discharged discharge after adsorption treatment.Thus it completes the preparation of complete environment culture solution and divides
With process.
By baffling device 11, rectangle circulation canal forms the circular passage of closure, is acted on by the water pumping of pump machine, makes ring
Water in shape channel can form circulating according to certain speed, will form the hydraulic pressure pressure difference of 11 two sides of baffling device,
Cause water to flow to tube body side from the side of the through-hole of funnel structure, the Daphnia magna in cultivation region can be drawn into square in this way
Shape circulation canal is simultaneously collected in water flow feeding collection device.
Collection device has the length in certain depth direction, and the discharge area of such collection device is bigger, and water is penetrated and gone
Resistance can reduce, will not influence the speed that in-channel water stream circulates in this way.
The structure of funnel collects Daphnia magna, and is sent in flow channel by tube body, so that subsequent water flow collects work
Make, funnel structure can cause water velocity to reach maximum in tube body, prevent Daphnia magna is adherent from causing to collect difficulty.In addition
The light of lighting apparatus can play the effect for luring Daphnia magna close, to be enriched with Daphnia magna in through hole in advance.
Pump machine can constantly drive the water flowing in flow channel, intermittent harvest Daphnia magna in such a way that interval starts.
By remove collection device can will collect Daphnia magna in collection device shift it is spare.Again by the water in cultivation region after allowing
It discharges, supplies new environment culture solution into cultivation region by environment culture solution supply dispatching unit 4 again again, then
It is put into Daphnia magna, then is detected.
As can be seen from the above embodiments, the present invention simulates nature receiving water body, tests the Daphnia magna in emulated ecological environment
By carrying out long term monitoring to water body to the sensitive response of pollutant toxicity and toxicant accumulation characteristic, after greatly facilitating
The research and analysis of continuous staff can make qualified discharge stain disease continuously enter experiment mould eventually by aforesaid way
In the container body of quasi- ecological environment, to realize the real-time and long-term comprehensive ecological Toxicity Monitoring of qualified discharge stain disease.
The present invention realizes preparing and automatically update and feed automatically for certain concentration environment culture solution；Realize Daphnia magna
The life processes such as development, breeding are come into full contact with and completed with environment culture solution, while avoiding escape and the machine of female Magna and children Magna
Tool damage.
The present invention realizes the processing such as filtering, the absorption of test waste water, reduces environmental hazard；Experimental provision is convenient for test behaviour
Make, is at low cost, being easy to laboratory popularization.
The above described is only a preferred embodiment of the present invention, being not that the invention has other forms of limitations, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But without departing from the technical solutions of the present invention, according to the technical essence of the invention to above embodiments institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection scope of technical solution of the present invention.
Claims (8)
1. a kind of water environmental factor based on Daphnia magna toxicity monitors experimental provision characterized by comprising Daphnia magna culture
Container (1), environment culture solution deployment unit (2), the storage of environment culture solution are with unit (3), environment culture solution supply dispatching unit
(4), waste disposal unit (5)；The Daphnia magna culture vessel (1), environment culture solution deployment unit (2), the storage of environment culture solution
It deposits and dispenses between unit (4) with unit (3) and the supply of environment culture solution respectively by environment culture solution supply dispatching unit (4) phase
Even, the Daphnia magna culture vessel (1) is connected with waste disposal unit (5)；
The Daphnia magna culture vessel (1) includes water inlet pipe, container body (6), lighting apparatus (7), water environmental factor monitoring
Unit (8), monitoring unit (9), water bath device (10) and outlet pipe；
In the water body environment of container body (6) formed experiment emulated ecological environment, the experiment emulated ecological environment include mud,
Water plant and Daphnia magna；Baffling device (11) are set in container body (6), container body (6) are separated by baffling device
The both ends of connection rectangle circulation canal, rectangle circulation canal fluid are distinguished in cultivation region and the part of race way two, race way both ends
Upstream end be equipped with water pump, downstream is equipped with collection device, and water pump and collection device are arranged in rectangle circulation canal；Barrier
Device (11) is equipped with the through-hole of several funnel structures, and wide opening one end of through-hole is located at cultivation region side, the slot one of through-hole
End is located at rectangle circulation canal side, and baffling device (11) is transparent configuration, around the slot of through-hole on be also provided in addition
Lighting apparatus.
2. the water environmental factor according to claim 1 based on Daphnia magna toxicity monitors experimental provision, which is characterized in that
The environment culture solution deployment unit (2) includes automatic liquid dispenser, cosolvent device, cosolvent control device for formulating, environment training
Nutrient solution device for formulating, the cosolvent device, cosolvent control device for formulating, environment culture solution device for formulating are respectively placed in independence
Magnetic stirring apparatus on.
3. the water environmental factor according to claim 1 to 2 based on Daphnia magna toxicity monitors experimental provision, feature exists
In the environment culture solution storage includes cosolvent control apparatus for temporary storage, environment culture solution apparatus for temporary storage with unit (3), above-mentioned
Cosolvent compares apparatus for temporary storage, the entrance of environment culture solution apparatus for temporary storage compares device for formulating, environment culture with cosolvent respectively
The outlet of liquid device for formulating is connected by pipeline, and pipeline is equipped with solenoid valve；All apparatus for temporary storage are brown glass material, and
Equipped with top cover, top cover apertured is inserted into for the perfusion tube of peristaltic pump.
4. the water environmental factor according to claim 3 based on Daphnia magna toxicity monitors experimental provision, which is characterized in that
Environment culture solution supply dispatching unit (4) includes liquid level sensor, automatic electromagnetic valve, peristaltic pump and PLC controller, is passed through
PLC controller blocks water flow or connection water flow automatically.
5. the water environmental factor described in -4 based on Daphnia magna toxicity monitors experimental provision according to claim 1, feature exists
In the waste disposal unit includes the filter cotton layer and activated carbon filter layer of Spaced setting, and it is single that the two is integrated in closing
In member, only it is connected by water inlet pipe with the outlet pipe of the Daphnia magna culture vessel (1).
6. the water environmental factor according to claim 5 based on Daphnia magna toxicity monitors experimental provision, which is characterized in that
The water inlet pipe connects the container body (6), for water to be monitored to be introduced the container body (6)；The illumination is set
Standby (7) setting carries out illumination to water body above the container body (6), for simulating daylight；The water environmental factor prison
Unit (8) are surveyed to be provided for detecting the water quality parameter of the water body；The monitoring unit (9) is arranged in the container body
(6) top, for monitoring the existing state of Daphnia magna in real time；The outlet pipe is connected to the container body (6), for arranging
Water and the volume substantially constant for keeping the water body.
7. the water environmental factor described in -6 based on Daphnia magna toxicity monitors experimental provision according to claim 1, feature exists
In launching in the cultivation region has enclosed grazing device, and the enclosed grazing device includes wrapping up grid, in the package grid
It is enclosed with feed and floating ball, fixed rope is provided on the enclosed grazing device, the fixed rope is posted in the container body (6)
Top.
8. the water environmental factor according to claim 7 based on Daphnia magna toxicity monitors experimental provision, which is characterized in that
The Daphnia magna culture vessel (1) further includes support frame and lamp bracket, and the container body (6) is arranged on support frame as described above,
The lamp bracket is supported on support frame as described above, for fixing the lighting apparatus (7)；The monitoring unit (9) is fixed on described
On lamp bracket, water bath device (10) setting is below the container body (6)；It further include timer, for controlling the photograph
The light application time of bright equipment (7).
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