CN219201412U - Biotoxicity detector - Google Patents
Biotoxicity detector Download PDFInfo
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- CN219201412U CN219201412U CN202223554989.XU CN202223554989U CN219201412U CN 219201412 U CN219201412 U CN 219201412U CN 202223554989 U CN202223554989 U CN 202223554989U CN 219201412 U CN219201412 U CN 219201412U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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- Y02A20/20—Controlling water pollution; Waste water treatment
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Abstract
The utility model discloses a biotoxicity detector, wherein a sampling unit comprises a first filter, a diaphragm pump and a water flow meter, a water sample processing unit comprises a second filter, a third filter, a constant temperature water storage tank, a fish peristaltic pump and a water flea peristaltic pump, a control unit comprises an MCU main control circuit board and a relay, an analysis data output unit comprises an industrial personal computer, the first filter is sequentially connected with the diaphragm pump and the water flow meter through a water inlet pipe, the water flow meter is connected with the constant temperature water storage tank, the bottom of the constant temperature water storage tank is further connected with an aeration device, the constant temperature water storage tank is further sequentially connected with a fish observation bin through the second filter and the fish peristaltic pump, the constant temperature water storage tank is further sequentially connected with the water flea observation bin through the third filter and the water flea peristaltic pump, and the constant temperature water storage tank is further connected with the fish observation bin and the water flea observation bin through a loop. The utility model has reasonable structural design, can effectively detect water quality toxicity by identifying the behavior of aquatic organisms, and has high detection precision, convenient and reliable use and strong practicability.
Description
Technical Field
The utility model relates to the technical field of water treatment and biological monitoring, in particular to a biotoxicity detector.
Background
The biological monitoring field needs to adopt biological fish, luminous bacteria, microorganisms and the like as indicator organisms to indicate the behavior change and behavior intensity index of the organisms, and the comprehensive toxicity of the water body is reflected by detection. The traditional biotoxicity detector has the advantages of limited functions, high requirements on indicated organisms, limited functions and low detection precision, and generally one detector can only aim at one indicated organism.
In summary, the utility model designs a biotoxicity detector compatible with the daphnia toxicity instrument and the fish toxicity instrument.
Disclosure of Invention
Aiming at the defects in the prior art, the utility model aims to provide the biotoxicity detector which is reasonable in structural design, high in detection precision, convenient and reliable to use and strong in practicability, and can effectively detect water quality toxicity by identifying the behaviors of aquatic organisms.
In order to achieve the above object, the present utility model is realized by the following technical scheme: the biotoxicity detector comprises a sampling unit, a water sample processing unit, a control unit and an analysis data output unit; the sampling unit comprises a first filter, a diaphragm pump and a water flow meter, the water sample treatment unit comprises a second filter, a third filter, a constant temperature water storage tank, a fish peristaltic pump and a water flea peristaltic pump, the control unit comprises an MCU main control circuit board and a relay, the analysis data output unit comprises an industrial personal computer, the first filter is sequentially connected with the diaphragm pump and the water flow meter through a water inlet pipe, the water flow meter is connected with the constant temperature water storage tank, the bottom of the constant temperature water storage tank is further connected with an aeration device, the constant temperature water storage tank is further sequentially connected with a fish observation bin through the second filter and the fish peristaltic pump, the constant temperature water storage tank is further sequentially connected with the water flea observation bin through the third filter and the water flea peristaltic pump, the fish observation bin is connected with a fish drain valve through a first electromagnetic valve, and the bottom of the constant temperature water storage tank is further connected with a constant temperature drain valve through a second electromagnetic valve and a constant temperature peristaltic pump; the first camera of storehouse is observed to the fish and the second camera in storehouse is observed to the water flea all links to each other with the industrial computer, and industrial computer, temperature sensor, high water level sensor, low water level sensor all link to each other with MCU main control circuit board, and MCU main control circuit board links to each other with relay, 4G transmission module, RS232 interface, fish drain valve, thermostat drain valve, sampling pump, drain pump respectively, and MCU main control circuit board still links to each other with fish peristaltic pump and water flea peristaltic pump through the drive plate respectively.
Preferably, the first filter, the second filter and the third filter are um metal filters: the filter is internally provided with a metal powder sintered filter core for preliminarily filtering impurities in water.
Preferably, the first electromagnetic valve and the second electromagnetic valve adopt DC24V normally closed electromagnetic valves to control the on-off of the pipelines.
Preferably, the constant-temperature water storage tank comprises a high water level sensor, a refrigerating block, a heating rod, a low water level sensor, an aeration device, a thermostat drain valve, a fish observation bin water inlet, a water flea observation bin water inlet, a water inlet to be tested, an air exhaust port, a water flea observation bin water return port and a fish observation bin water return port, the upper part and the bottom of the constant-temperature water storage tank are respectively provided with the high water level sensor and the low water level sensor, the two sides of the constant-temperature water storage tank are respectively provided with the refrigerating block, and the bottom of the constant-temperature water storage tank is provided with the heating rod, the aeration device and the thermostat drain valve; the top of the constant temperature water storage tank is provided with a fish observation bin water inlet, a water flea observation bin water inlet, a water inlet to be measured, an evacuation port, a water flea observation bin water return port and a fish observation bin water return port.
Preferably, the water flea observation bin comprises a first water inlet connected with a water inlet of the water flea observation bin, a first water return port connected with a water return port of the water flea observation bin, a water flea interaction area is arranged below the first water inlet and the first water return port, blue specific light sources are arranged on two sides of the water flea interaction area, a darkroom is arranged below the water flea interaction area, and a second camera is arranged on one side of the darkroom.
Preferably, the fish observation bin is arranged on a sliding rail, the sliding rail is arranged on the rack in a sliding manner, a first light source and a second light source are respectively arranged above and below the fish observation bin, and a first camera is arranged above the first light source.
The utility model has the beneficial effects that: the utility model has reasonable structural design, convenient and reliable use, high precision and strong practicability, and can effectively analyze and detect the toxicity of water quality through biological behaviors.
Drawings
The utility model is described in detail below with reference to the drawings and the detailed description;
FIG. 1 is a waterway diagram of the present utility model;
FIG. 2 is a circuit block diagram of the present utility model;
FIG. 3 is a schematic perspective view of a constant temperature water storage tank of the present utility model;
FIG. 4 is a longitudinal cross sectional view of the constant temperature water storage tank of the present utility model;
FIG. 5 is a schematic view of the water flea observation bin of the present utility model mated with a darkroom;
FIG. 6 is a cross-sectional view of FIG. 5;
fig. 7 is a schematic view of the closed fish observation bin of the present utility model.
Fig. 8 is a schematic view of the opened body of the fish observation bin of the present utility model.
Detailed Description
The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.
Referring to fig. 1-8, the present embodiment adopts the following technical scheme: the biotoxicity detector comprises a sampling unit, a water sample processing unit, a control unit and an analysis data output unit; the sampling unit comprises a first filter 1, a diaphragm pump 4 and a water flow meter 5, the water sample processing unit comprises a second filter 2, a third filter 3, a constant temperature water storage tank 6, a fish peristaltic pump 7 and a flea peristaltic pump 8, the control unit comprises an MCU main control circuit board 9 and a relay 10, the analysis data output unit comprises an industrial personal computer 11, the first filter 1 is sequentially connected with the diaphragm pump 4 and the water flow meter 5 through a water inlet pipe, the water flow meter 5 is connected with the constant temperature water storage tank 6, the bottom of the constant temperature water storage tank 6 is further connected with an aeration device 12, the constant temperature water storage tank 6 is further sequentially connected with a fish observation bin 13 through the second filter 2 and the fish peristaltic pump 7, the constant temperature water storage tank 6 is further sequentially connected with a flea observation bin 14 through the third filter 3 and the flea peristaltic pump 8, the constant temperature water storage tank 6 is connected with the fish observation bin 13 and the water flea observation bin 14 through loops, the fish observation bin 13 is connected with a water drain valve 16 through a first electromagnetic valve 15, and the bottom of the constant temperature water storage tank 6 is further connected with a constant temperature drain valve 18 through a second electromagnetic valve 17 and a constant temperature peristaltic pump 21; the first camera 19 of the fish observing bin 13 and the second camera 20 of the water flea observing bin 14 are connected with the industrial personal computer 11, the temperature sensor, the high water level sensor and the low water level sensor are connected with the MCU main control circuit board 9, the MCU main control circuit board 9 is respectively connected with the relay 10, the 4G transmission module, the RS232 interface, the fish drain valve 16, the thermostat drain valve 18, the sampling pump and the drainage pump, and the MCU main control circuit board 9 is also respectively connected with the fish peristaltic pump 7 and the water flea peristaltic pump 8 through driving boards.
The filter in the sampling unit preliminarily filters sundries in water. The diaphragm pump pumps water, the water flow meter can observe the pumping condition, and the congestion possibly caused by sundries in water can be found in time, so that the problem is solved.
The water sample treatment unit is characterized in that the water sample treatment unit is provided with water delivery power by two 48um filters, a constant temperature water storage tank and two peristaltic pumps.
The control unit: the instrument adopts a full-automatic operation mode, the core of the control instrument is a 32-bit control main board, and the control instrument is matched with an intermediate relay to realize output control. Electromagnetic valves are arranged on each path of the sampling pipe and used as executing elements, so that the functions of automatic sampling, periodic control, water drainage and the like are realized. A step of
The analysis data output unit: an industrial personal computer is arranged in the instrument to collect and analyze various data.
The first filter 1, the second filter 2 and the third filter 3 adopt 80um metal filters: the filter is internally provided with a metal powder sintered filter core for preliminarily filtering impurities in water.
The first electromagnetic valve 15 and the second electromagnetic valve 17 adopt DC24V normally closed electromagnetic valves to control the on-off of pipelines.
The constant-temperature water storage tank 6 comprises a high water level sensor 61, a refrigerating block 62, a heating rod 63, a low water level sensor 64, an aeration device 65, a thermostat drain valve 18, a fish observation bin water inlet 66, a water flea observation bin water inlet 67, a water body inlet 68 to be detected, an exhaust air port 69, a water flea observation bin water return port 610 and a fish observation bin water return port 611, the upper part and the bottom of the constant-temperature water storage tank 6 are respectively provided with the high water level sensor 61 and the low water level sensor 64, both sides of the constant-temperature water storage tank 6 are provided with the refrigerating block 62, and the bottom of the constant-temperature water storage tank 6 is provided with the heating rod 63, the aeration device 65 and the thermostat drain valve 18; the top of the constant temperature water storage tank 6 is provided with a fish observation bin water inlet 66, a water flea observation bin water inlet 67, a water inlet 68 to be measured, an air exhaust port 69, a water flea observation bin water return port 610 and a fish observation bin water return port 611. The constant temperature water storage tank is controlled to be 25 ℃ by a heating and refrigerating device.
The water flea observation bin 14 comprises a first water inlet 141 connected with a water flea observation bin water inlet 67, a first water return port 142 connected with a water flea observation bin water return port 610, a water flea interaction area 143 is arranged below the first water inlet 141 and the first water return port 142, blue specific light sources 145 are arranged on two sides of the water flea interaction area 143, a darkroom 144 is arranged below the water flea interaction area 143, and a second camera 20 is arranged on one side of the darkroom 144.
The fish observation bin 13 is arranged on the sliding rail 131, the sliding rail 131 is arranged on the rack 132 in a sliding mode, a first light source 133 and a second light source 134 are respectively arranged above and below the fish observation bin 13, and a first camera 19 is arranged above the first light source 133.
The fish observation bin 13 and the flea observation bin 14 of the specific embodiment are analyzed by utilizing microscopic shooting technology, image identification tracking and uploading to an upper computer software. Peristaltic pumps and diaphragm pumps provide power for water body transmission, DC24V and maximum transmission flow rate is 600ml/min. The water flow meter is used for observing the water flow state during sampling. The oxygen content of the sample water body is increased by the gas explosion device. The constant temperature water storage tank is used for carrying out treatments such as temperature rise, temperature reduction, oxygenation and the like on the water body to be detected in a fixed container. The water body to be measured reaches a relatively constant state before entering the observation bin, and the influence on the biological sample caused by the problems of water body temperature, oxygen content and the like is eliminated. The water flea observation bin consists of a bin barrel, a camera, a light source, a water flea observation bin, a water inlet, a water return port and the like. Because the daphnia body type is relatively small, the daphnia body needs to be injected in an absolute darkroom through specific lamp light, and errors caused by factors such as shadow and unclear observation are eliminated. And observing the water flea movement in the observation bin by a microscopic shooting technology.
The detection principle of the specific embodiment: under a relatively constant environment, the pollution degree of the water body is judged and measured through real-time shooting of aquatic organisms in the bin and the change of the kinematic behaviors of the aquatic organisms.
After the water fleas are damaged by toxic substances, the behaviors such as irregular movement patterns, reduced movement speed and the like can appear. The water quality toxicity is calculated according to the model by shooting the behavior changes of living body number, swimming speed, swimming amplitude, growth speed, population distribution and the like of the daphnia and transmitting the data to a computer.
The test method for zebra fish is similar to the daphnia described above.
The biotoxicity detector monitors the influence of water on aquatic organisms in real time by utilizing a microscopic shooting technology, an image tracking technology, an automatic control technology and a computer software technology.
The detection flow of the specific embodiment is as follows: and (5) extracting the water body to be detected, and enabling the water body to be detected to enter a constant-temperature water storage tank through the 80um metal filter. Filtering impurities through a 48um filter after constant temperature, and then entering each observation bin,
1. opening the observation bin, putting the aquatic biological sample into the observation bin, and filling the water flea observation bin with water as much as possible;
2. setting specific parameters of a sample, sampling times and the like in a computer, and starting a manual/automatic mode.
3. Starting a diaphragm pump to pump the water body to be measured into a constant-temperature water storage tank;
4. and when the water pumping water level reaches the upper limit of the water level, the water storage tank carries out constant temperature treatment on the water body.
5. When the water body reaches the set temperature, the peristaltic pump for fish and the peristaltic pump for water fleas are started, the water body to be tested enters the observation bin for fish and water fleas through the filter 2/3, and returns to the constant-temperature water storage tank through the loop when the observation bin is full of water, so that the purpose of water body circulation is achieved, and the relatively constant living environment of aquatic organisms is ensured. Simultaneously, the first camera, the second camera and the light source are started to shoot the observation bin. The shot images are transmitted into a computer in real time, the computer analyzes the movement behaviors of the aquatic organisms in real time according to the transmitted images, and the analysis results are displayed in real time in a curve and data mode.
6. After one observation is completed (the observation time is set by itself), a drainage program is started, the fish peristaltic pump and the water flea peristaltic pump stop working, the constant-temperature equipment in the constant-temperature water storage tank is closed, the first electromagnetic valve, the second electromagnetic valve and the constant-temperature peristaltic pump are opened, and the wastewater is discharged (if only the water flea is observed, only the second electromagnetic valve and the constant-temperature peristaltic pump are opened). When the water level in the constant-temperature water storage tank reaches the lowest water level line, stopping the water drainage program, starting the diaphragm pump to pump water, and entering the next observation experiment. The observation times are set by oneself.
The functions of this embodiment are as follows:
1. the motion state of the zebra fish and the fleas is displayed in real time, and the motion amplitude, the motion speed and the motion range are displayed. The identification rate, size, survival number and toxicity value of water quality are monitored.
2. The action of the electromagnetic valve and the water pump is controlled, the upper limit and the lower limit of the temperature are checked and set in real time, the water circulation of the water fleas and the zebra fish is realized, and the water quality is tested.
3. The basic information of the current test can be checked, and the test log is automatically updated.
4. And drawing a toxicity value curve, a biological movement amplitude curve, a movement speed curve, a movement range curve, a recognition rate curve, a growth rate curve and the like of water quality in real time.
5. The test data is checked, screened and displayed in a curve form.
6. And managing the test information and the calibration information.
7. And (5) setting a toxicity alarm.
8. Data may be derived.
The foregoing has shown and described the basic principles and main features of the present utility model and the advantages of the present utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (6)
1. The biotoxicity detector is characterized by comprising a sampling unit, a water sample processing unit, a control unit and an analysis data output unit; the sampling unit include first filter (1), diaphragm pump (4) and water flow meter (5), water sample processing unit include second filter (2), third filter (3), constant temperature water storage tank (6), fish peristaltic pump (7) and water flea peristaltic pump (8), the control unit include MCU master control circuit board (9) and relay (10), analysis data output unit include industrial computer (11), first filter (1) link to each other in proper order through inlet tube and diaphragm pump (4), water flow meter (5) link to each other with constant temperature water storage tank (6), constant temperature water storage tank (6) bottom still links to each other with aeration equipment, constant temperature water storage tank (6) still link to each other with fish observation storehouse (13) through second filter (2), fish peristaltic pump (7) in proper order, constant temperature water storage tank (6) still link to each other with water flea observation storehouse (14) through third filter (3), water flea peristaltic pump (8), constant temperature water storage tank (6) are through loop connection observation storehouse (13) and water storage storehouse (14), constant temperature water storage tank (6) are through solenoid valve (16) and water storage tank (17) are observed with fish inspection storehouse (13) in proper order, water storage tank (6) is through the solenoid valve (16) bottom is observed with fish discharge valve (13) The thermostat peristaltic pump (21) is connected with the thermostat drain valve (18); the first camera (19) of storehouse (13) is observed to fish and second camera (20) of storehouse (14) are observed to the water flea all link to each other with industrial computer (11), temperature sensor, high water level sensor, low water level sensor all link to each other with MCU master control circuit board (9), MCU master control circuit board (9) respectively with relay (10), 4G transmission module, RS232 interface, fish drain valve (16), thermostat drain valve (18), sampling pump, drain pump link to each other, MCU master control circuit board (9) still link to each other with fish peristaltic pump (7) and water flea peristaltic pump (8) through the drive plate respectively.
2. The biotoxicity detector of claim 1, wherein the first filter (1), the second filter (2) and the third filter (3) are 80um metal filters.
3. The biotoxicity detector of claim 1, wherein the first electromagnetic valve (15) and the second electromagnetic valve (17) adopt DC24V normally closed electromagnetic valves to control on-off of pipelines.
4. The biotoxicity detector of claim 1, wherein the constant temperature water storage tank (6) comprises a high water level sensor (61), a refrigerating block (62), a heating rod (63), a low water level sensor (64), an aeration device (65), a thermostat drain valve (18), a fish observation bin water inlet (66), a water flea observation bin water inlet (67), a water inlet to be detected (68), an air exhaust port (69), a water flea observation bin water return port (610) and a fish observation bin water return port (611), the high water level sensor (61) and the low water level sensor (64) are respectively arranged at the upper part and the bottom of the constant temperature water storage tank (6), the refrigerating block (62) is arranged at two sides of the constant temperature water storage tank (6), and the heating rod (63), the aeration device (65) and the thermostat drain valve (18) are arranged at the bottom of the constant temperature water storage tank (6); the top of the constant temperature water storage tank (6) is provided with a fish observation bin water inlet (66), a water flea observation bin water inlet (67), a water inlet (68) to be measured, an air exhaust port (69), a water flea observation bin water return port (610) and a fish observation bin water return port (611).
5. The biotoxicity detector of claim 1, wherein the water flea observation bin (14) comprises a first water inlet (141) connected with a water flea observation bin water inlet (67), a first water return port (142) connected with a water flea observation bin water return port (610), a water flea interaction area (143) is arranged below the first water inlet (141) and the first water return port (142), blue specific light sources (145) are arranged on two sides of the water flea interaction area (143), a darkroom (144) is arranged below the water flea interaction area (143), and a second camera (20) is arranged on one side of the darkroom (144).
6. The biotoxicity detector of claim 1, wherein the fish observation bin (13) is arranged on a sliding rail (131), the sliding rail (131) is slidably arranged on a rack (132), a first light source (133) and a second light source (134) are respectively arranged above and below the fish observation bin (13), and a first camera (19) is arranged above the first light source (133).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223554989.XU CN219201412U (en) | 2022-12-29 | 2022-12-29 | Biotoxicity detector |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202223554989.XU CN219201412U (en) | 2022-12-29 | 2022-12-29 | Biotoxicity detector |
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| CN219201412U true CN219201412U (en) | 2023-06-16 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118655134A (en) * | 2024-08-16 | 2024-09-17 | 山东省林业科学研究院 | Biological alarm device for monitoring water content |
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- 2022-12-29 CN CN202223554989.XU patent/CN219201412U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118655134A (en) * | 2024-08-16 | 2024-09-17 | 山东省林业科学研究院 | Biological alarm device for monitoring water content |
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