CN109827956A - A kind of automatic detection device and detection method of ammonia nitrogen content of nitrite - Google Patents
A kind of automatic detection device and detection method of ammonia nitrogen content of nitrite Download PDFInfo
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Abstract
The invention belongs to technical field of aquaculture, specifically disclose the automatic detection device and detection method of a kind of ammonia nitrogen content of nitrite.The automatic detection device includes being sequentially connected logical sample introduction component, buffer unit, reaction part and observation component；The first peristaltic pump is equipped between the outlet of the buffer unit and the entrance of reaction part；Two-way peristaltic pump is equipped between the outlet of the reaction part and the entrance of observation component；The entrance of the reaction part is also connected with several reagent components；Composite light source and multiband luminosity induction module are equipped in the observation component.Automatic detection device of the invention can quickly and accurately detect the content of ammonia nitrogen and nitrite in breeding water body simultaneously, and whole process realizes automatic detection, do not need manual intervention, be effectively saved cost of labor.
Description
Technical field
The present invention relates to technical field of aquaculture, and in particular, to a kind of automation inspection of ammonia nitrogen content of nitrite
Survey device and detection method.
Background technique
China's culture fishery is quickly grown in recent years, and intensive high-density breeding becomes development trend, however also causes
Breeding ecological environment is destroyed.It is unreasonable due to not focusing on planning, breeding way, blindly pursue high density and high yield, mistake
Amount uses feed and drug, seriously exceeds water body bearing capacity, fish disease and quality problem are constantly serious.Drug and feed it is residual
It stays, the accumulation of the excreta, dead volume and Organic residues of biology, water body is caused seriously to pollute, dissolved oxygen decline, ammonia nitrogen, nitrite
Increase with harmful substances such as hydrogen sulfide, COD increases, and there is also a degree of heavy metal pollution phenomenons.Water quality it is good
The bad health for directly influencing aquatic animal, with going deep into for environmental health cultivation idea, the monitoring and management of cultivation water at
For one of the emphasis of daily aquaculture.
For the content of pollutant ammonia nitrogen and nitrite common in aquaculture system, mainstream detection mode is at present
It is detected using kit or Test paper, this method needs manual sampling, after being put into reagent reaction, uses artificial visual
Colorimetric method judges ammonia nitrogen/nitrite concentration in water body.But the problem of this method, is, first is that manual operation is complicated,
High labor cost；Second is that visual colorimetric determination is easy to produce deviation, precision is low.
In addition, the content of ammonia nitrogen and nitrite is also detected frequently with portable ammonia nitrogen/nitrite detection device, with
The detection method of kit or Test paper is compared, it is still necessary to reagent reaction is sampled and added by manual mode of operation, only
Water sample after reaction is to carry out colorimetric by equipment, reduces the error of artificial colorimetric.
Although automatic detection system has that error is small, advantage with high accuracy, but seldom adopt in aquaculture field at present
With, main reason is that: first is that equipment is costly；Second is that single device can only detect a Xiang Zhibiao, two indexs all need to examine
Survey needs to purchase two different equipment, further improves use cost.To sum up, still lack at present it is a kind of efficiently it is convenient,
Error is small, precision is high, save cost of labor, multinomial pollutant index can be detected simultaneously automatic detection system and detection side
Method.
Summary of the invention
The purpose of the invention is to overcome the shortcomings of that existing automatic detection system can only detect Single Pollution object index,
A kind of automatic detection device of ammonia nitrogen content of nitrite is provided, can quickly and accurately detect ammonia nitrogen in breeding water body simultaneously
With the content of nitrite.
Another object of the present invention is to provide a kind of detection methods of ammonia nitrogen content of nitrite.
To achieve the goals above, the present invention is achieved by following scheme:
A kind of automatic detection device of ammonia nitrogen content of nitrite, including being sequentially connected logical sample introduction component, buffer part
Part, reaction part and observation component；The first peristaltic pump is equipped between the outlet of the buffer unit and the entrance of reaction part；Institute
It states and is equipped with two-way peristaltic pump between the outlet of reaction part and the entrance of observation component；The entrance of the reaction part also with it is several
A reagent component is connected；Composite light source and multiband luminosity induction module are equipped in the observation component.
Since aquaculture system is typically more muddy, it is more to include all kinds of suspended matters, and the present invention passes through setting sample introduction portion
Part can filter out most of impurity, promote the accuracy of detection, while can reduce the risks such as equipment pollution, pipeline blockage,
Reduce the requirement of regular maintenance.By the way that buffer unit is arranged, first is that as transfer, because sample introduction component flow is big, it is difficult to essence
Really control water withdrawal, needs to carry out second decimation；Second is that further progress contamination precipitation, only takes upper layer water sample to be pumped into reacting part
Part.By the way that the first peristaltic pump is arranged, for the water sample in buffer unit to be pumped into reaction part, accurately controlled by extracting the time
Extraction amount processed.By the way that several reagent components are arranged, reagent can be accurately added into reaction part, due to detection ammonia nitrogen and nitrous
Reagent needed for hydrochlorate is different, according to test-types, different reagents can be extracted from different reagent components and is added to reacting part
In part, the purpose for quickly, accurately completing Measuring Several Indexes is realized simultaneously.By the way that two-way peristaltic pump is arranged, for water sample to be existed
It is mutually mobile between reaction part and observation component.It is multiple for irradiating water sample to be measured by the way that composite light source is arranged in observation component
The light source of different frequency range needed for ammonia nitrogen, nitrite detection are contained in light combination source can provide different frequency range according to test-types
Light source.By the way that multiband luminosity induction module is arranged in observation component, for reading the light intensity of different frequency range in water sample, water sample
After addition reagent reaction, corresponding reaction color can be showed, so that water body color burn (concentration is higher, and color is deeper), extinction
Degree will increase, thus react after light intensity value can be reduced than initial beam intensity, therefore by judge light intensity reduction number
The concentration of water sample is extrapolated in turn.Since water sample color is different after ammonia nitrogen, nitrite reaction, not when reading light intensity
It can difference, the value for selecting light intensity value to change maximum frequency range in calculated result have been calculated with the variation of frequency range light intensity value,
Different concentration can be more accurately distinguished, detection accuracy is improved.
Preferably, the sample introduction component is made of the fore filter and immersible pump being connected, the outlet connection of immersible pump
The entrance of buffer unit.The immersible pump is used for the water sample automatically taking water directly remote from distance, drop is big, such as directly from fishpond
Extract water sample.Immersible pump can according to need setting multichannel, realize that an equipment can be completed from multiple and different fishpond automatically taking waters
The requirement of test.
Preferably, the sample introduction component is made of the fore filter being connected and the second peristaltic pump, the second peristaltic pump
The entrance of outlet connection buffer unit.Second peristaltic pump is used to be tested from nearby water sample automatically taking water, such as is placed on
The sampling cup etc. of desktop.
Preferably, the outside of the reaction part is equipped with thermostat.The thermostat provides heated at constant temperature for reaction part
Condition shortens the reaction time.
Preferably, the inside of the reaction part is equipped with blender.The blender after adding reagent for being stirred
It mixes, it is ensured that reaction uniformly carries out.
Preferably, the reagent component is made of the reagent bottle and reagent pump being connected, and the outlet of reagent pump connects reaction
The entrance of component.
It is highly preferred that the reagent pump is bidirectional rotation.After having added reagent, is pumped by reversal agents and carries out pumpback,
Remaining reagent in Reagent Tube is drawn back in reagent bottle, because portion of reagent is stood for a long time is possible to generate the feelings such as precipitating, crystallization
Condition is easy blocking Reagent Tube, can be to avoid these situations by reagent pumpback.
A kind of detection method of ammonia nitrogen content of nitrite is also claimed in the present invention, utilizes above-mentioned automatic detection device
It is detected, comprising the following steps:
S1. water sample to be measured is extracted to buffer unit from sample introduction component, carry out transfer precipitating, by the first peristaltic pump from buffering
Component extracts quantitative water sample to reaction part；
S2. quantitative reagent is extracted to reaction part from reagent component, be uniformly mixed with water sample to be measured, carry out chromogenic reaction；
S3. water sample component from is extracted from reaction part by two-way peristaltic pump, opens composite light source, passes through multiband
Luminosity induction module reads light intensity after water sample reaction, calculates containing for ammonia nitrogen or nitrite in water sample by built-in standard curve
Amount.
Using detection method of the present invention, single device can be completed to carry out ammonia nitrogen, nitrite two to multichannel water body
The automatic detection of item index；Evaded by preheating, time constant-temperature mode because weather conditions lead to the water sample reaction time not
Unanimous circumstances；The design separated using reaction part with observation component is avoided because the reaction time is too long, so that locular wall is dirty
Dye causes reading color inaccurate；It is irradiated by composite light source, multiband luminosity induction module frequency-division section reads light intensity value, chooses most suitable
Frequency range light intensity calculates testing result, realizes that single device detects two ammonia nitrogen, nitrite indexs；It is calculated and is detected by light intensity ratio
As a result, shielding initial water color difference is different, the accuracy of detection is promoted.
Compared with prior art, the invention has the following advantages:
(1) automatic detection device of the invention can quickly and accurately detect ammonia nitrogen and nitrous acid in breeding water body simultaneously
The content of salt, whole process realize automatic detection, do not need manual intervention, be effectively saved cost of labor.
(2) detection method of the invention is effectively mentioned by using modes such as prefilter, heated at constant temperature, light intensity ratio calculating
The high speed and accuracy of detection；Reduce the blocked risk of Reagent Tube by practical pumpback mode, reduce maintenance difficulties,
Be conducive to final-period management and maintenance, can be applicable in aquaculture field.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of 1 automatic detection device of embodiment.
Fig. 2 is the structural schematic diagram of 2 automatic detection device of embodiment.
Fig. 3 is the structural schematic diagram of 3 automatic detection device of embodiment.
Fig. 4 is 4 Plays concentration curve of embodiment.
Appended drawing reference: 1- sample introduction component；2- buffer unit；3- reaction part；4- observes component；The first peristaltic pump of 5-；6-
Two-way peristaltic pump；7- reagent component；8- thermostat；9- blender；11- fore filter；12- immersible pump；13- second is wriggled
Pump；41- composite light source；42- multiband luminosity induction module；71- reagent bottle；72- reagent pump.
Specific embodiment
With reference to the accompanying drawings of the specification and specific embodiment is made the present invention and is further elaborated, the embodiment
It is served only for explaining the present invention, be not intended to limit the scope of the present invention.Test method as used in the following examples is such as without spy
Different explanation, is conventional method；Used material, reagent etc., unless otherwise specified, for the reagent commercially obtained
And material.
Embodiment 1
A kind of automatic detection device of ammonia nitrogen content of nitrite, including being sequentially connected logical sample introduction component 1, buffer part
Part 2, reaction part 3 and observation component 4；Sample introduction component 1 is to be connected by two parallel branches with buffer unit 2, a branch
It is made of the fore filter 11 and immersible pump 12 that are connected, the entrance of the outlet connection buffer unit 2 of immersible pump 12 is another
Branch is made of the fore filter 11 being connected and the second peristaltic pump 13, and the outlet of the second peristaltic pump 13 connects buffer part
The entrance of part 2；The first peristaltic pump 5 is equipped between the outlet of buffer unit 2 and the entrance of reaction part 3；The outlet of reaction part 3
Two-way peristaltic pump 6 is equipped between the entrance of observation component 4；The entrance of reaction part 3 is also connected with two reagent components 7,
Reagent component 7 is made of the reagent bottle 71 and reagent pump 72 being connected, the entrance of the outlet connection reaction part 3 of reagent pump 72,
Reagent pump 72 is bidirectional rotation；The outside of reaction part 3 is equipped with thermostat 8；The inside of reaction part 3 is equipped with blender 9；Observation
Composite light source 41 and multiband luminosity induction module 42 are equipped in component 4.
Since aquaculture system is typically more muddy, it is more to include all kinds of suspended matters, can be with by setting sample introduction component 1
Most of impurity is filtered out, promotes the accuracy of detection, while the risks such as equipment pollution, pipeline blockage can be reduced, reduces warp
The requirement of perseverance maintenance.
By the way that immersible pump 12 is arranged, for the water sample automatically taking water directly remote from distance, drop is big, such as directly from fishpond
Extract water sample.Immersible pump 12 can according to need setting multichannel, realize that an equipment can be complete from multiple and different fishpond automatically taking waters
At the requirement of test.
By the way that the second peristaltic pump 13 is arranged, for being tested from nearby water sample automatically taking water, such as it is placed on taking for desktop
Sample cup etc..
By the way that buffer unit 2 is arranged, first is that as transfer, because 1 flow of sample introduction component is big, it is difficult to accurate control water intaking
Amount, needs to carry out second decimation；Second is that further progress contamination precipitation, only takes upper layer water sample to be pumped into reaction part 3.
By the way that the first peristaltic pump 5 is arranged, for the water sample in buffer unit 2 to be pumped into reaction part 3, when passing through extraction
Between accurately control extraction amount.
By the way that two-way peristaltic pump 6 is arranged, for water sample to be mutually mobile between reaction part 3 and observation component 4.
By the way that two reagent components 7 are arranged, reagent can be accurately added into reaction part 3, due to detection ammonia nitrogen and nitrous
Reagent needed for hydrochlorate is different, according to test-types, can extract different reagents from different reagent components 7 and be added to reacting part
In part 3, the purpose for quickly, accurately completing Measuring Several Indexes is realized simultaneously.
By the way that the reagent pump 72 of bidirectional rotation is arranged, after having added reagent, pumpback is carried out by reversal agents pump 72, it will
Remaining reagent in Reagent Tube is drawn back in reagent bottle 71, because portion of reagent is stood for a long time is possible to generate the feelings such as precipitating, crystallization
Condition is easy blocking Reagent Tube, can be to avoid these situations by reagent pumpback.
It is that reaction part 3 provides heated at constant temperature condition by setting thermostat 8, the reaction time can be shortened, reach quick inspection
The purpose of survey.
It is used to be stirred after adding reagent by setting blender 9, it is ensured that reaction uniformly carries out, and further reaches fast
Speed detection, the purpose for improving detection accuracy.
By the way that composite light source 41 is arranged in observation component 4, for irradiating water sample to be measured, composite light source 41 containing ammonia nitrogen,
The light source of different frequency range needed for nitrite detection can provide the light source of different frequency range according to test-types.
By the way that multiband luminosity induction module 42 is arranged in observation component 4, for reading the light of different frequency range in water sample
By force, water sample can show corresponding reaction color, so that (the higher color of concentration is more for water body color burn after addition reagent reaction
It is deep), absorbance will increase, therefore the light intensity value after reaction can be reduced than initial beam intensity, therefore by judging that light intensity is reduced more
The concentration of water sample can be extrapolated in turn less.Since water sample color is different after ammonia nitrogen, nitrite reaction, reading
When light intensity the variation of different frequency range light intensity value can difference, selected in calculated result light intensity value change the value of maximum frequency range into
Row calculates, and can more accurately distinguish different concentration, improves detection accuracy.
Ammonia nitrogen and nitrous in breeding water body can quickly and accurately be detected using the automatic detection device of the present embodiment simultaneously
The content of hydrochlorate, whole process realize automatic detection, do not need manual intervention, be effectively saved cost of labor.
Embodiment 2
A kind of automatic detection device of ammonia nitrogen content of nitrite, including being sequentially connected logical sample introduction component 1, buffer part
Part 2, reaction part 3 and observation component 4；Sample introduction component 1 is made of the fore filter 11 and immersible pump 12 being connected, immersible pump
The entrance of 12 outlet connection buffer unit 2；First is equipped between the outlet of buffer unit 2 and the entrance of reaction part 3 to wriggle
Pump 5；Two-way peristaltic pump 6 is equipped between the outlet of reaction part 3 and the entrance of observation component 4；The entrance of reaction part 3 is also with two
A reagent component 7 is connected, and reagent component 7 is made of the reagent bottle 71 and reagent pump 72 being connected, and the outlet of reagent pump 72 connects
The entrance of reaction part 3 is connect, reagent pump 72 is bidirectional rotation；The outside of reaction part 3 is equipped with thermostat 8；Reaction part 3 it is interior
Portion is equipped with blender 9；It observes and is equipped with composite light source 41 and multiband luminosity induction module 42 in component 4.
Since aquaculture system is typically more muddy, it is more to include all kinds of suspended matters, can be with by setting sample introduction component 1
Most of impurity is filtered out, promotes the accuracy of detection, while the risks such as equipment pollution, pipeline blockage can be reduced, reduces warp
The requirement of perseverance maintenance.
By the way that immersible pump 12 is arranged, for the water sample automatically taking water directly remote from distance, drop is big, such as directly from fishpond
Extract water sample.Immersible pump 12 can according to need setting multichannel, realize that an equipment can be complete from multiple and different fishpond automatically taking waters
At the requirement of test.
By the way that buffer unit 2 is arranged, first is that as transfer, because 1 flow of sample introduction component is big, it is difficult to accurate control water intaking
Amount, needs to carry out second decimation；Second is that further progress contamination precipitation, only takes upper layer water sample to be pumped into reaction part 3.
By the way that the first peristaltic pump 5 is arranged, for the water sample in buffer unit 2 to be pumped into reaction part 3, when passing through extraction
Between accurately control extraction amount.
By the way that two-way peristaltic pump 6 is arranged, for water sample to be mutually mobile between reaction part 3 and observation component 4.
By the way that two reagent components 7 are arranged, reagent can be accurately added into reaction part 3, due to detection ammonia nitrogen and nitrous
Reagent needed for hydrochlorate is different, according to test-types, can extract different reagents from different reagent components 7 and be added to reacting part
In part 3, the purpose for quickly, accurately completing Measuring Several Indexes is realized simultaneously.
By the way that the reagent pump 72 of bidirectional rotation is arranged, after having added reagent, pumpback is carried out by reversal agents pump 72, it will
Remaining reagent in Reagent Tube is drawn back in reagent bottle 71, because portion of reagent is stood for a long time is possible to generate the feelings such as precipitating, crystallization
Condition is easy blocking Reagent Tube, can be to avoid these situations by reagent pumpback.
It is that reaction part 3 provides heated at constant temperature condition by setting thermostat 8, the reaction time can be shortened, reach quick inspection
The purpose of survey.
It is used to be stirred after adding reagent by setting blender 9, it is ensured that reaction uniformly carries out, and further reaches fast
Speed detection, the purpose for improving detection accuracy.
By the way that composite light source 41 is arranged in observation component 4, for irradiating water sample to be measured, composite light source 41 containing ammonia nitrogen,
The light source of different frequency range needed for nitrite detection can provide the light source of different frequency range according to test-types.
By the way that multiband luminosity induction module 42 is arranged in observation component 4, for reading the light of different frequency range in water sample
By force, water sample can show corresponding reaction color, so that (the higher color of concentration is more for water body color burn after addition reagent reaction
It is deep), absorbance will increase, therefore the light intensity value after reaction can be reduced than initial beam intensity, therefore by judging that light intensity is reduced more
The concentration of water sample can be extrapolated in turn less.Since water sample color is different after ammonia nitrogen, nitrite reaction, reading
When light intensity the variation of different frequency range light intensity value can difference, selected in calculated result light intensity value change the value of maximum frequency range into
Row calculates, and can more accurately distinguish different concentration, improves detection accuracy.
Ammonia nitrogen and nitrous in breeding water body can quickly and accurately be detected using the automatic detection device of the present embodiment simultaneously
The content of hydrochlorate, whole process realize automatic detection, do not need manual intervention, be effectively saved cost of labor.
Embodiment 3
A kind of automatic detection device of ammonia nitrogen content of nitrite, including being sequentially connected logical sample introduction component 1, buffer part
Part 2, reaction part 3 and observation component 4；Sample introduction component 1 is made of the fore filter 11 being connected and the second peristaltic pump 13, the
The entrance of the outlet connection buffer unit 2 of two peristaltic pumps 13；It is equipped between the outlet of buffer unit 2 and the entrance of reaction part 3
First peristaltic pump 5；Two-way peristaltic pump 6 is equipped between the outlet of reaction part 3 and the entrance of observation component 4；Reaction part 3 enters
Mouth is also connected with two reagent components 7, and reagent component 7 is made of the reagent bottle 71 and reagent pump 72 being connected, reagent pump 72
Outlet connection reaction part 3 entrance, reagent pump 72 be bidirectional rotation；The outside of reaction part 3 is equipped with thermostat 8；Reaction
The inside of component 3 is equipped with blender 9；It observes and is equipped with composite light source 41 and multiband luminosity induction module 42 in component 4.
Since aquaculture system is typically more muddy, it is more to include all kinds of suspended matters, can be with by setting sample introduction component 1
Most of impurity is filtered out, promotes the accuracy of detection, while the risks such as equipment pollution, pipeline blockage can be reduced, reduces warp
The requirement of perseverance maintenance.
By the way that the second peristaltic pump 13 is arranged, for being tested from nearby water sample automatically taking water, such as it is placed on taking for desktop
Sample cup etc..
By the way that buffer unit 2 is arranged, first is that as transfer, because 1 flow of sample introduction component is big, it is difficult to accurate control water intaking
Amount, needs to carry out second decimation；Second is that further progress contamination precipitation, only takes upper layer water sample to be pumped into reaction part 3.
By the way that the first peristaltic pump 5 is arranged, for the water sample in buffer unit 2 to be pumped into reaction part 3, when passing through extraction
Between accurately control extraction amount.
By the way that two-way peristaltic pump 6 is arranged, for water sample to be mutually mobile between reaction part 3 and observation component 4.
By the way that two reagent components 7 are arranged, reagent can be accurately added into reaction part 3, due to detection ammonia nitrogen and nitrous
Reagent needed for hydrochlorate is different, according to test-types, can extract different reagents from different reagent components 7 and be added to reacting part
In part 3, the purpose for quickly, accurately completing Measuring Several Indexes is realized simultaneously.
By the way that the reagent pump 72 of bidirectional rotation is arranged, after having added reagent, pumpback is carried out by reversal agents pump 72, it will
Remaining reagent in Reagent Tube is drawn back in reagent bottle 71, because portion of reagent is stood for a long time is possible to generate the feelings such as precipitating, crystallization
Condition is easy blocking Reagent Tube, can be to avoid these situations by reagent pumpback.
It is that reaction part 3 provides heated at constant temperature condition by setting thermostat 8, the reaction time can be shortened, reach quick inspection
The purpose of survey.
It is used to be stirred after adding reagent by setting blender 9, it is ensured that reaction uniformly carries out, and further reaches fast
Speed detection, the purpose for improving detection accuracy.
By the way that composite light source 41 is arranged in observation component 4, for irradiating water sample to be measured, composite light source 41 containing ammonia nitrogen,
The light source of different frequency range needed for nitrite detection can provide the light source of different frequency range according to test-types.
By the way that multiband luminosity induction module 42 is arranged in observation component 4, for reading the light of different frequency range in water sample
By force, water sample can show corresponding reaction color, so that (the higher color of concentration is more for water body color burn after addition reagent reaction
It is deep), absorbance will increase, therefore the light intensity value after reaction can be reduced than initial beam intensity, therefore by judging that light intensity is reduced more
The concentration of water sample can be extrapolated in turn less.Since water sample color is different after ammonia nitrogen, nitrite reaction, reading
When light intensity the variation of different frequency range light intensity value can difference, selected in calculated result light intensity value change the value of maximum frequency range into
Row calculates, and can more accurately distinguish different concentration, improves detection accuracy.
Ammonia nitrogen and nitrous in breeding water body can quickly and accurately be detected using the automatic detection device of the present embodiment simultaneously
The content of hydrochlorate, whole process realize automatic detection, do not need manual intervention, be effectively saved cost of labor.
Embodiment 4
A kind of detection method of ammonia nitrogen content of nitrite, is detected using automatic detection device described in embodiment 1,
The following steps are included:
S1. extract water sample to be measured to buffer unit 2 from sample introduction component 1, carry out transfer precipitating, by the first peristaltic pump 5 from
Buffer unit 2 extracts quantitative water sample to reaction part 3；
S2. quantitative reagent is extracted to reaction part 3 from reagent component 7, be uniformly mixed with water sample to be measured, develop the color anti-
It answers；
S3. water sample component 4 from are extracted from reaction part 3 by two-way peristaltic pump 6, opens composite light source 41, passes through
Multiband luminosity induction module 42 reads light intensity after water sample reaction, calculates ammonia nitrogen or nitrous acid in water sample by built-in standard curve
The content of salt.
The concrete operations of above-mentioned detection method are as follows:
(1) according to the difference of dry point (fishpond or sampling cup), excess is extracted by immersible pump 12 or the second peristaltic pump 13
Water sample is to buffer unit 2；
(2) part water sample is drawn by reaction part 3 by the first peristaltic pump 5, for cleaning reaction part 3, before avoiding
The water sample residual of face test；
(3) 3 water sample of reaction part is emptied；
(4) quantitative water sample is drawn by reaction part 3 by the first peristaltic pump 5, then empties the remaining water of buffer unit 2
Sample；
(5) water sample of reaction part 3 is drawn by observation component 4 by two-way peristaltic pump 6；
(6) composite light source 41 in observation component 4 is opened, and initial by the reading water sample of multiband luminosity induction module 42
Light intensity (takes multiple averaging value)；
(7) by two-way peristaltic pump 6, by water sample, water sample is drawn back reaction part 3 by the component 4 from；
(8) start constant temperature heating device (i.e. thermostat 8), water sample is pre-heated to assigned temperature, and (water sample temperature is different, instead
It is also different the time required to answering；Preheating can guarantee that under the conditions of different weather, the water sample temperature before adding reagent is consistent, guarantee
Reaction primary condition it is consistent, to guarantee the stability of test result)；
(9) different reagent pumps 72 is driven in order according to test-types different (ammonia nitrogen or nitrite), addition is different
Reagent, different reagents are accurately controlled according to required amount by the control addition time；
(10) control blender 9 is stirred water sample, it is ensured that reagent is uniformly mixed with water sample；
(11) (reagent pump 72 does not contact liquid level in reaction part 3, and antiport only can for driving reagent pump 72 progress pumpback
Air in abstraction reaction component 3, while the reagent in Reagent Tube being drawn back in reagent bottle 71), it is ensured that Reagent Tube will not block up
Plug；
(12) according to different test-types, 8 continuous heating specified time of thermostat, it is ensured that (heating time is completed in reaction
It is needed according to the reaction time of the required time decision of reagent reaction, such as ammonia nitrogen longer than nitrite)；
(13) after the reaction was completed, the water sample of reaction part 3 is drawn by observation component 4 by two-way peristaltic pump 6；
(14) composite light source 41 in observation component 4 is opened, and anti-by the reading water sample of multiband luminosity induction module 42
It should rear light intensity (taking multiple averaging value)；
(15) by two-way peristaltic pump 6, by water sample, water sample is extracted back reaction part 3 by the component 4 from；
(16) 3 waste liquid of reaction part is emptied, completes test.
Through detecting, the color after ammonia nitrogen reaction can become blue, detect the variation of frequency range light intensity near 440nm；Nitrite
It can redden after reaction, detect the variation of frequency range light intensity near 660nm.
The calculating of final result:
Due to may be more muddy in breeding water body, color be different, thus cannot directly using the light intensity value after reacting as
Calculation basis is used as calculation basis using light intensity ratio (light intensity value after water sample reaction before light intensity value/water sample reaction).Together
When ammonia nitrogen, nitrite can be according to respectively needing to select the light intensity value of different frequency range as calculation basis.
The titer for configuring various concentration first, is demarcated under various concentration conditions, and light intensity ratio is how many respectively, thus quasi-
Close out a standard concentration curve, it is counter from standard concentration curve to look into corresponding end value according to light intensity ratio when test.Example
Such as, the following table 1 is the calibration sample table of ammonia nitrogen titer.According to table 1 fit come standard concentration curve it is as shown in Figure 4.
The calibration sample table of 1 ammonia nitrogen titer of table
| Ammonia nitrogen concentration of standard solution (mg/L) | Light intensity ratio |
| 0.2 | 0.8397 |
| 0.4 | 0.7461 |
| 0.6 | 0.6738 |
| 0.8 | 0.6037 |
| 1 | 0.5526 |
| 1.5 | 0.4716 |
| 2 | 0.3153 |
| 2.5 | 0.2423 |
| 3 | 0.1848 |
| 3.5 | 0.1472 |
| 4 | 0.1106 |
If the light intensity ratio that a water sample tests out is 0.4, it can reversely show that ammonia nitrogen contains by above-mentioned curve
Amount is 1.73.
Using the detection method of the present embodiment, single device can be completed to carry out ammonia nitrogen, nitrite to multichannel water body
The automatic detection of two indexs；Evaded by preheating, time constant-temperature mode because weather conditions lead to the water sample reaction time
Inconsistent situation；The design separated using reaction part with observation component is avoided because the reaction time is too long, so that locular wall quilt
Pollution causes reading color inaccurate；It is irradiated by composite light source, multiband luminosity induction module frequency-division section reads light intensity value, and selection is most closed
Suitable frequency range light intensity calculates testing result, realizes that single device detects two ammonia nitrogen, nitrite indexs；It is calculated and is examined by light intensity ratio
Survey as a result, shielding initial water color difference it is different, improve the accuracy of detection.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of shield range can also be made on the basis of above description and thinking for those of ordinary skill in the art
Other various forms of variations or variation, there is no necessity and possibility to exhaust all the enbodiments.It is all of the invention
Made any modifications, equivalent replacements, and improvements etc., should be included in the protection of the claims in the present invention within spirit and principle
Within the scope of.
Claims (8)
1. a kind of automatic detection device of ammonia nitrogen content of nitrite, which is characterized in that including being sequentially connected logical sample introduction portion
Part (1), buffer unit (2), reaction part (3) and observation component (4)；The outlet and reaction part (3) of the buffer unit (2)
Entrance between be equipped with the first peristaltic pump (5)；It is equipped between the outlet of the reaction part (3) and the entrance for observing component (4)
Two-way peristaltic pump (6)；The entrance of the reaction part (3) is also connected with several reagent components (7)；The observation component
(4) composite light source (41) and multiband luminosity induction module (42) are equipped in.
2. automatic detection device according to claim 1, which is characterized in that before the sample introduction component (1) is by being connected
Set filter (11) and immersible pump (12) composition, the entrance of outlet connection buffer unit (2) of immersible pump (12).
3. automatic detection device according to claim 1, which is characterized in that before the sample introduction component (1) is by being connected
Set filter (11) and the second peristaltic pump (13) composition, the entrance of outlet connection buffer unit (2) of the second peristaltic pump (13).
4. automatic detection device according to claim 1, which is characterized in that the outside of the reaction part (3) is equipped with perseverance
Warm device (8).
5. automatic detection device according to claim 1, which is characterized in that the inside of the reaction part (3) is equipped with and stirs
Mix device (9).
6. automatic detection device according to claim 1, which is characterized in that the reagent component (7) is by the examination that is connected
Agent bottle (71) and reagent pump (72) composition, the entrance of outlet connection reaction part (3) of reagent pump (72).
7. automatic detection device according to claim 6, which is characterized in that the reagent pump (72) is bidirectional rotation.
8. a kind of detection method of ammonia nitrogen content of nitrite, which is characterized in that utilize described in any one of claim 1 to 7 certainly
Dynamicization detection device is detected, comprising the following steps:
S1. water sample to be measured is extracted to buffer unit (2) from sample introduction component (1), carry out transfer precipitating, pass through the first peristaltic pump (5)
Quantitative water sample is extracted to reaction part (3) from buffer unit (2)；
S2. quantitative reagent is extracted to reaction part (3) from reagent component (7), be uniformly mixed with water sample to be measured, develop the color anti-
It answers；
S3. water sample component (4) from are extracted from reaction part (3) by two-way peristaltic pump (6), opened composite light source (41),
By multiband luminosity induction module (42) read water sample reaction after light intensity, by built-in standard curve calculate water sample in ammonia nitrogen or
The content of nitrite.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111474170A (en) * | 2020-05-22 | 2020-07-31 | 广州丁海科技有限公司 | Detection system and method for detecting calcium and magnesium ion content and total alkalinity of water body |
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2019
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111474170A (en) * | 2020-05-22 | 2020-07-31 | 广州丁海科技有限公司 | Detection system and method for detecting calcium and magnesium ion content and total alkalinity of water body |
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