CN105277542B - A kind of water nitrite field fast detection method for eliminating reagent blank influence - Google Patents

A kind of water nitrite field fast detection method for eliminating reagent blank influence Download PDF

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CN105277542B
CN105277542B CN201510061828.2A CN201510061828A CN105277542B CN 105277542 B CN105277542 B CN 105277542B CN 201510061828 A CN201510061828 A CN 201510061828A CN 105277542 B CN105277542 B CN 105277542B
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nitrite
water
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CN105277542A (en
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连国军
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Wenzhou Medical University
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Abstract

The invention discloses a kind of water nitrite field fast detection method for eliminating reagent blank influence, this method by the preparation of liquid-type nitrite detection reagent, deduct reagent blank color and using transparent plastic sheet for the standard color card of support material making, detect when blank tube color addition is compareed on standard color card with sample cell again.Method is simple, accuracy is high, easily operated, is detected using liquid-type nitrite detection reagent, the time required to can effectively controlling whole detection process;Method by the way that blank tube color addition to be carried out to colorimetric control with sample cell again on standard color card, the fluctuation that can effectively eliminate reagent blank adversely affect to caused by testing result accuracy, are particularly suitable for water nitrite field quick detection.

Description

A kind of water nitrite field fast detection method for eliminating reagent blank influence
Technical field
The invention belongs to field of water quality detection, more particularly to a kind of water nitrite for eliminating reagent blank influence to show Field quick determination method.
Background technology
Nitrite especially in vaporous water, surface water and groundwater and dynamic is planted universally present in nature In object and food.Nitrite has been acknowledged as a major pollutants index in our daily life drinking-water, and this refers to Mark reflects the degree polluted in water by nitrogenous type organic to a certain extent.The material will trigger after human body is entered Methemoglobinemia, there is common histanoxia symptom, carcinogen can also be combined into secondary amine class material, seriously Influence and threaten the health of the mankind.Danger based on nitrite is widely present, and state health standards are clearly advised NO in Drinking Water is determined2 -≤1.0mg/L.The high drinking water of long-term intake nitrite concentration, nitrite meeting and human body Protein combines to form the nitrosamine with strong carcinogenicity, and this material can cause cancer of the esophagus, stomach cancer, liver cancer and colorectal cancer etc..
The detection of water nitrite more using ultraviolet spectrophotometry, visible spectrophotometry, chemoluminescence method, from Sub- chromatography etc., these methods are both needed to taking back water sample into special laboratory, are chemically examined and completed by the operating technology personnel of specialty, greatly Part Methods also need to the high large-scale instrumentation of price, it is impossible to meet quick, easy Site Detection requirement, it is special at some Different when and where also there is a situation where to sample not in time, sampling location Water quality can not be objectively responded.
Existing nitrite Site Detection reagent is mostly the dry-type detection examination based on colorimetric method in the market Agent, the detection reagent take a certain amount of water sample mix, dissolve with dry-type detection reagent when measuring, react a period of time After produce specific color, the concentration for drawing water sample test substance is directly compareed with standard color card.Such method is quick, conveniently, Public participation degree is high, but influence of the change to measurement result of reagent blank can not be eliminated during measurement, only permanent in reagent blank Just there is higher accuracy when fixed constant.But the detection of actually detected discovery, either liquid-type or dry-type tries Agent, that reagent blank becomes larger or diminished all inevitably be present in storing process.In addition, use dry-type , it is necessary to first detection reagent is fully dissolved with water sample to be measured when detection reagent carries out water sample detection, then with the specific dirt in water sample Dye thing carries out reaction and produces particular color.Because the TWS that Various Seasonal, different zones gather is not quite similar, dry powder is dissolved Also it is not quite similar the time required to during type detection reagent, is difficult to effectively control the time required to whole detection process, also to testing result Accuracy adversely affect.
Therefore, demand it is a kind of using liquid-type detection reagent, reagent blank can be eliminated testing result is influenceed, using standard The detection method of the quantitative water nitrite of the direct visual colorimetric determination of colour atla, for realizing water nitrite field quick detection It is particularly important.
The content of the invention
The purpose of the embodiment of the present invention is to provide a kind of water nitrite scene for eliminating reagent blank influence soon Fast detection method, to realize the live quick, Accurate Determining of water nitrite, prior art is overcome using dry-type detection examination It is difficult to effectively control detection time because of the difference of dissolution time during agent, does not deduct the standard color card of reagent blank because using, compares This standard color card is directly compareed with sample cell during color obtain a result, the fluctuation that can not eliminate reagent blank it is accurate to testing result The shortcomings that property adversely affects.
The present invention is achieved in that a kind of water nitrite field quick detection side for eliminating reagent blank influence Method includes:
Step 1: preparing liquid-type nitrite detection reagent, the detection reagent is containing p-aminobenzene sulfonic acid, hydrochloric acid naphthalene The Acetic acid-sodium acetate buffer solution that ethylenediamine, emulsifier op-10, the pH of ethylene glycol are 5.0;
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark Quasi- pipe, the nitrite standard liquid of deionized water and various concentrations is added in blank tube and standard pipe, adds step respectively Liquid-type nitrite detection reagent described in rapid one, reaction a period of time;
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software It is after each standard pipe color is deducted blank tube color by photoshop7.0, the color of gained and corresponding nitrite standard is molten The concentration of liquid is printed upon on water white transparency PVC plastic flitch, obtains deducting the nitrite standard color card after reagent blank;
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample QC, deionized water and water sample to be measured are added in blank tube and sample cell respectively, adds the liquid-type described in step 1 Nitrite detection reagent, reaction a period of time;
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when blank tube is superimposed with standard color card When color afterwards is with sample cell solid colour, the nitrite concentration corresponding to standard color card is read within a certain period of time, is Water sample nitrite concentration.
Further, in step 1, vinegar that pH that described liquid-type nitrite detection reagent is 80mmol/L is 5.0 Acid-sodium-acetate buffer, the concentration of the p-aminobenzene sulfonic acid contained is 2-5g/L, the concentration of hydrochloride naphthodiamide is 0.2- 0.5g/L, the concentration of emulsifier op-10 are 0.1-0.3ml/L, the concentration of ethylene glycol is 10-20ml/L, and compound method is:Weigh 2-5g p-aminobenzene sulfonic acid, 0.2-0.5g hydrochloride naphthodiamides, add 800ml 80mmol/L pH5.0 Acetic acid-sodium acetate buffer solutions Stirring and dissolving, 0.1-0.3ml emulsifier op-10s, 10-20ml ethylene glycol are added, 80mmol/L pH5.0 are used after stirring and dissolving Acetic acid-sodium acetate buffer solution is settled to 1000ml.
Further, in step 2, the concentration of described nitrite standard liquid be respectively 0.05mg/L, 0.10mg/L, 0.20mg/L, 0.30mg/L, 0.40mg/L, 0.50mg/L, compound method are:Precision weighs 0.20g top pure grade natrium nitrosums, adds Deionized water dissolving is simultaneously settled to 1000ml, and it is 200mg/L nitrite (in terms of natrium nitrosum) storing solution to obtain concentration, then is used Deionized water by nitrite storing solution be diluted to concentration for 0.05mg/L, 0.10mg/L, 0.20mg/L, 0.30mg/L, 0.40mg/L, 0.50mg/L nitrite (in terms of natrium nitrosum) standard liquid.
Further, in step 2 and step 4, the nitrite standard of described addition deionized water and various concentrations is molten The volume of liquid is 10ml.
Further, in step 2 and step 4, the volume of described addition liquid-type nitrite detection reagent is 0.2ml。
Further, in step 2 and step 4, preferred reaction conditions are that 5-15 minutes are reacted in 5-40 DEG C of environment.
Further, in step 3, the thickness of described water white transparency PVC plastic flitch is 1-2mm.
Further, in step 5, described certain time is 5-10 minutes.
The nitrite field fast detection method for eliminating reagent blank influence of the present invention has method simple, accurate The advantages of property is high, easily operated;Detected using liquid-type nitrite detection reagent, can effectively control whole detection process Required time;Method by the way that blank tube color addition to be carried out to colorimetric control with sample cell again on standard color card, can be effective Influence of the reagent blank to testing result is eliminated, is particularly suitable for water nitrite field quick detection.
Brief description of the drawings
Fig. 1 is the water nitrite field quick detection side provided in an embodiment of the present invention for eliminating reagent blank influence Method flow chart.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, book is sent out It is bright to be further elaborated.It should be appreciated that specific embodiment described herein is only to explain this present invention, not For limiting the present invention.
Below in conjunction with the accompanying drawings and specific embodiment is further described to the application principle of the present invention.
As shown in figure 1, the present invention is achieved in that a kind of water nitrite scene for eliminating reagent blank influence Quick determination method includes:
S101, liquid-type nitrite detection reagent is prepared, the detection reagent is containing p-aminobenzene sulfonic acid, hydrochloric acid naphthalene second The Acetic acid-sodium acetate buffer solution that diamines, emulsifier op-10, the pH of ethylene glycol are 5.0;
S102,7 10ml glass colorimetric cylinders are taken, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are standard Pipe, the nitrite standard liquid of deionized water and various concentrations is added in blank tube and standard pipe respectively, adds step Liquid-type nitrite detection reagent described in S101, reaction a period of time;
S103, the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, use mapping software It is after each standard pipe color is deducted blank tube color by photoshop7.0, the color of gained and corresponding nitrite standard is molten The concentration of liquid is printed upon on water white transparency PVC plastic flitch, obtains deducting the nitrite standard color card after reagent blank;
S104,2 10ml glass colorimetric cylinders are separately taken, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample Pipe, deionized water and water sample to be measured are added in blank tube and sample cell respectively, adds the liquid-type described in step S101 Nitrite detection reagent, reaction a period of time;
S105, the blank bottom of the tube that the standard color card described in step S103 is placed in step 4, from colorimetric bottom of the tube to It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when blank tube is superimposed with standard color card When color afterwards is with sample cell solid colour, the nitrite concentration corresponding to standard color card is read within a certain period of time, is Water sample nitrite concentration.
Further, in step S101, vinegar that pH that described liquid-type nitrite detection reagent is 80mmol/L is 5.0 Acid-sodium-acetate buffer, the concentration of the p-aminobenzene sulfonic acid contained is 2-5g/L, the concentration of hydrochloride naphthodiamide is 0.2- 0.5g/L, the concentration of emulsifier op-10 are 0.1-0.3ml/L, the concentration of ethylene glycol is 10-20ml/L, and compound method is:Weigh 2-5g p-aminobenzene sulfonic acid, 0.2-0.5g hydrochloride naphthodiamides, add 800ml 80mmol/L pH5.0 Acetic acid-sodium acetate buffer solutions Stirring and dissolving, 0.1-0.3ml emulsifier op-10s, 10-20ml ethylene glycol are added, 80mmol/L pH5.0 are used after stirring and dissolving Acetic acid-sodium acetate buffer solution is settled to 1000ml.
Further, in step S102, the concentration of described nitrite standard liquid is respectively 0.05mg/L, 0.10mg/ L, 0.20mg/L, 0.30mg/L, 0.40mg/L, 0.50mg/L, compound method are:Precision weighs 0.20g top pure grade natrium nitrosums, Add deionized water dissolving and be settled to 1000ml, it is 200mg/L nitrite (in terms of natrium nitrosum) storing solution to obtain concentration, then With deionized water by nitrite storing solution be diluted to concentration be 0.05mg/L, 0.10mg/L, 0.20mg/L, 0.30mg/L, 0.40mg/L, 0.50mg/L nitrite (in terms of natrium nitrosum) standard liquid.
Further, in step S102 and step S104, the nitrite mark of described addition deionized water and various concentrations The volume of quasi- solution is 10ml.
Further, in step S102 and step S104, the volume of described addition liquid-type nitrite detection reagent is 0.2ml。
Further, in step S102 and step S104, preferred reaction conditions are that 5-15 minutes are reacted in 5-40 DEG C of environment.
Further, in step S103, the thickness of described water white transparency PVC plastic flitch is 1-2mm.
Further, in step S105, described certain time is 5-10 minutes.
The specific embodiment of the present invention:
Embodiment 1
Step 1: weighing 2g p-aminobenzene sulfonic acid, 0.2g hydrochloride naphthodiamides, add 800ml 80mmol/L pH5.0 vinegar Acid-sodium-acetate buffer stirring and dissolving, 0.1ml emulsifier op-10s, 10ml ethylene glycol are added, 80mmol/ is used after stirring and dissolving L pH5.0 Acetic acid-sodium acetate buffer solutions are settled to 1000ml, obtain 1000ml and contain 2g/L p-aminobenzene sulfonic acid, 0.2g/L salt Sour naphthodiamide, 0.1ml/L emulsifier op-10s, the 80mmol/L pH5.0 Acetic acid-sodium acetate buffer solutions of 10ml/L ethylene glycol are Liquid-type nitrite detection reagent;
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark Quasi- pipe, added respectively in blank tube and standard pipe 10ml deionized waters and 10ml concentration be 0.05mg/L, 0.10mg/L, 0.20mg/L, 0.30mg/L, 0.40mg/L, 0.50mg/L nitrite standard liquid, add the liquid described in step 1 Type nitrite detection reagent 0.2ml, reacted 15 minutes in 5 DEG C of environment;
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software It is after each standard pipe color is deducted blank tube color by photoshop7.0, the color of gained and corresponding nitrite standard is molten The concentration of liquid is printed upon on the water white transparency PVC plastic flitch that thickness is 1mm, obtains deducting each concentration nitrous acid after reagent blank Salt standard color card;
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample QC, 10ml deionized waters and 10ml water samples to be measured are added in blank tube and sample cell respectively, is added described in step 1 Liquid-type nitrite detection reagent, reacted 15 minutes in 5 DEG C of environment;
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when both solid colours, at 5 points The nitrite concentration corresponding to standard color card is read in clock, it is 0.05mg/L to draw water sample nitrite concentration.
Nitrous acid with national standard method hydrochloride naphthodiamide AAS to water sample described in the present embodiment step 4 Salinity is detected, and is as a result 0.048mg/L, it can be seen that the present embodiment methods described and hydrochloride naphthodiamide light splitting light The testing result of degree method has good uniformity.
Embodiment 2
Step 1: weighing 3.5g p-aminobenzene sulfonic acid, 0.35g hydrochloride naphthodiamides, add 800ml 80mmol/L pH5.0 Acetic acid-sodium acetate buffer solution stirring and dissolving, 0.2ml emulsifier op-10s, 15ml ethylene glycol are added, is used after stirring and dissolving 80mmol/L pH5.0 Acetic acid-sodium acetate buffer solutions are settled to 1000ml, obtain 1000ml contain 3.5g/L p-aminobenzene sulfonic acid, 0.35g/L hydrochloride naphthodiamides, 0.2ml/L emulsifier op-10s, 80mmol/L pH5.0 acetic acid-acetic acid of 15ml/L ethylene glycol Sodium buffer solution is liquid-type nitrite detection reagent;
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark Quasi- pipe, added respectively in blank tube and standard pipe 10ml deionized waters and 10ml concentration be 0.05mg/L, 0.10mg/L, 0.20mg/L, 0.30mg/L, 0.40mg/L, 0.50mg/L nitrite standard liquid, add the liquid described in step 1 Type nitrite detection reagent 0.2ml, reacted 10 minutes in 20 DEG C of environment;
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software It is after each standard pipe color is deducted blank tube color by photoshop7.0, the color of gained and corresponding nitrite standard is molten The concentration of liquid is printed upon on the water white transparency PVC plastic flitch that thickness is 1.5mm, obtains deducting each concentration nitrous after reagent blank Hydrochlorate standard color card;
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample QC, 10ml deionized waters and 10ml water samples to be measured are added in blank tube and sample cell respectively, is added described in step 1 Liquid-type nitrite detection reagent, reacted 10 minutes in 20 DEG C of environment;
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when both solid colours, 7.5 The nitrite concentration corresponding to standard color card is read in minute, it is 0.30mg/L to draw water sample nitrite concentration.
Nitrous acid with national standard method hydrochloride naphthodiamide AAS to water sample described in the present embodiment step 4 Salinity is detected, and is as a result 0.32mg/L, it can be seen that the present embodiment methods described and hydrochloride naphthodiamide spectrophotometric The testing result of method has good uniformity.
Embodiment 3
Step 1: weighing 5g p-aminobenzene sulfonic acid, 0.5g hydrochloride naphthodiamides, add 800ml 80mmol/L pH5.0 vinegar Acid-sodium-acetate buffer stirring and dissolving, 0.3ml emulsifier op-10s, 20ml ethylene glycol are added, 80mmol/ is used after stirring and dissolving L pH5.0 Acetic acid-sodium acetate buffer solutions are settled to 1000ml, obtain 1000ml and contain 5g/L p-aminobenzene sulfonic acid, 0.5g/L salt Sour naphthodiamide, 0.3ml/L emulsifier op-10s, the 80mmol/L pH5.0 Acetic acid-sodium acetate buffer solutions of 20ml/L ethylene glycol are Liquid-type nitrite detection reagent;
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark Quasi- pipe, added respectively in blank tube and standard pipe 10ml deionized waters and 10ml concentration be 0.05mg/L, 0.10mg/L, 0.20mg/L, 0.30mg/L, 0.40mg/L, 0.50mg/L nitrite standard liquid, add the liquid described in step 1 Type nitrite detection reagent 0.2ml, reacted 5 minutes in 40 DEG C of environment;
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software It is after each standard pipe color is deducted blank tube color by photoshop7.0, the color of gained and corresponding nitrite standard is molten The concentration of liquid is printed upon on the water white transparency PVC plastic flitch that thickness is 2mm, obtains deducting each concentration nitrous acid after reagent blank Salt standard color card;
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample QC, 10ml deionized waters and 10ml water samples to be measured are added in blank tube and sample cell respectively, is added described in step 1 Liquid-type nitrite detection reagent, reacted 5 minutes in 40 DEG C of environment;
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when both solid colours, at 10 points The nitrite concentration corresponding to standard color card is read in clock, it is 0.50mg/L to draw water sample nitrite concentration.
Nitrous acid with national standard method hydrochloride naphthodiamide AAS to water sample described in the present embodiment step 4 Salinity is detected, and is as a result 0.49mg/L, it can be seen that the present embodiment methods described and hydrochloride naphthodiamide spectrophotometric The testing result of method has good uniformity.
Embodiment 4
In order to further prove the creativeness of the present invention, the present invention has done following contrast test, has investigated the change of reagent blank Change the influence to measurement result accuracy.Method as described in the step 1 of this law embodiment 1 prepares the detection of liquid-type nitrite Reagent is test group, not deduct reagent blank, use the detection reagent of standard color card direct color comparison as control group, by two groups of examinations 4 DEG C of refrigerators are put in agent respectively and 37 water-baths store 7 days, and to using hydrochloride naphthodiamide spectrophotometry nitrous with a Hydrochlorate concentration is that 0.05mg/L water sample is measured, while replaces water sample to carry out reagent blank test with deionized water, with 721 Spectrophotometer records reagent blank value, and cuvette optical path be 1cm during test, test wavelength 550nm, reference solution be go from Sub- water, the results are shown in Table 1.
Table 1
As it can be seen from table 1 under identical experiment condition, with the gradual increase of reagent blank, the measure of test group As a result keep constant, but the measurement result of control group substantially becomes big, shows that method of the present invention can effectively remove reagent sky Influence of the white change to nitrite test result.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.

Claims (10)

1. a kind of water nitrite field fast detection method for eliminating reagent blank influence, it is characterised in that described The water nitrite field fast detection method of reagent blank influence, which can be eliminated, to be included:Step 1: prepare liquid-type nitrous Hydrochlorate detection reagent, the detection reagent are containing p-aminobenzene sulfonic acid, hydrochloride naphthodiamide, emulsifier op-10, ethylene glycol PH is 5.0 acetic acid-sodium-acetate buffer;Step 2: taking 7 10ml glass colorimetric cylinders, the 1st is set Colorimetric cylinder is blank tube, 2-7 root colorimetric cylinders are standard pipe, adds deionized water and not in blank tube and standard pipe respectively With the nitrite standard liquid of concentration, the liquid-type nitrite detection reagent described in step 1 is added, is reacted; Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding nitrite standard The concentration of solution is printed upon on water white transparency PVC plastic plates, obtains deducting the nitrite standard color card after reagent blank; Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample QC, deionized water and water sample to be measured, the liquid-type nitrous added in step 1 are added in blank tube and sample cell respectively Hydrochlorate detection reagent, is reacted;Step 5: the blank bottom of the tube that the standard color card of step 3 is placed in step 4, by The bottom up observation blank tube of colorimetric cylinder be superimposed with standard color card after color, and with sample cell color comparator, when blank tube and During color and sample cell solid colour after standard color card superposition, the nitrite concentration corresponding to standard color card is read, is Water sample nitrite concentration.
2. the water nitrite field fast detection method for eliminating reagent blank influence as described in claim 1, its It is characterised by, in step 1, acetic acid that pH that described liquid-type nitrite detection reagent is 80mmol/L is 5.0 - sodium-acetate buffer, the concentration of the p-aminobenzene sulfonic acid contained is 2-5g/L, the concentration of hydrochloride naphthodiamide is 0.2- 0.5g/L, the concentration of emulsifier op-10 are 0.1-0.3ml/L, the concentration of ethylene glycol is 10-20ml/L.
3. the water nitrite field fast detection method for eliminating reagent blank influence as described in claim 2, its It is characterised by, acetic acid-sodium acetate buffer liquid making method is:Weigh 2-5g p-aminobenzene sulfonic acid, 0.2-0.5g hydrochloric acid Naphthodiamide, add 800ml 80mmol/L pH5.0 acetic acid-sodium-acetate buffer stirring and dissolving, add 0.1-0.3ml Emulsifier op-10,10-20ml ethylene glycol, it is settled to 80mmol/L pH5.0 Acetic acid-sodium acetate buffer solutions after stirring and dissolving 1000ml。
4. the water nitrite field fast detection method for eliminating reagent blank influence as described in claim 1, its Be characterised by, in step 2, the concentration of described nitrite standard liquid be respectively 0.05mg/L, 0.10mg/L, 0.20mg/L、0.30mg/L、0.40mg/L、0.50mg/L。
5. the water nitrite field fast detection method for eliminating reagent blank influence as described in claim 4, its It is characterised by, nitrite standard liquid compound method is:0.20g top pure grade natrium nitrosums are weighed, add deionized water dissolving And 1000ml is settled to, it is 200mg/L nitrite storing solutions to obtain concentration, then is laid in nitrite with deionized water Liquid is diluted to the nitrous acid that concentration is 0.05mg/L, 0.10mg/L, 0.20mg/L, 0.30mg/L, 0.40mg/L, 0.50mg/L Salt standard liquid.
6. the water nitrite field fast detection method for eliminating reagent blank influence as described in claim 1, its It is characterised by, in step 2, the volume of the nitrite standard liquid of described addition deionized water and various concentrations is 10ml, in step 4, the volume of described addition deionized water is 10ml.
7. the water nitrite field fast detection method for eliminating reagent blank influence as described in claim 1, its It is characterised by, in step 2 and step 4, the volume of described addition liquid-type nitrite detection reagent is 0.2ml.
8. the water nitrite field fast detection method for eliminating reagent blank influence as described in claim 1, its It is characterised by, in step 2 and step 4, reaction condition is that 5-15 minutes are reacted in 5-40 DEG C of environment.
9. the water nitrite field fast detection method for eliminating reagent blank influence as described in claim 1, its It is characterised by, in step 3, the thickness of described water white transparency PVC plastic plates is 1-2mm.
10. the water nitrite field fast detection method for eliminating reagent blank influence as described in claim 1, its It is characterised by, the nitrite concentration corresponding to standard color card is read in step 5, after reaction 5-10 minutes.
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