CN105277540B - Copper ion field fast detection method in a kind of water for eliminating reagent blank influence - Google Patents

Copper ion field fast detection method in a kind of water for eliminating reagent blank influence Download PDF

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CN105277540B
CN105277540B CN201510061817.4A CN201510061817A CN105277540B CN 105277540 B CN105277540 B CN 105277540B CN 201510061817 A CN201510061817 A CN 201510061817A CN 105277540 B CN105277540 B CN 105277540B
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copper ion
water
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CN105277540A (en
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连国军
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Wenzhou Medical University
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Abstract

The invention discloses copper ion field fast detection method in a kind of water for eliminating reagent blank influence, this method by the preparation of liquid-type copper ion detection reagent, deduct reagent blank color and to be compareed blank tube color addition with sample cell again on standard color card when making, detection of the transparent plastic sheet for the standard color card of support material, with the advantages of method is simple, accuracy is high, easily operated;Detected using liquid-type copper ion detection reagent, the time required to can effectively controlling whole detection process;Method by the way that blank tube color addition to be carried out to colorimetric control with sample cell again on standard color card, the fluctuation that can effectively eliminate reagent blank adversely affect to caused by testing result accuracy, are particularly suitable for copper ion field quick detection in water.

Description

Copper ion field fast detection method in a kind of water for eliminating reagent blank influence
Technical field
The invention belongs to copper ion scene in field of water quality detection, more particularly to a kind of water for eliminating reagent blank influence Quick determination method.
Background technology
Copper (Cu) is the essential trace elements of the human body, there is many-sided physiological function inside people.Research has shown that, drinking water In micro copper be beneficial to human body, the deficiency of copper in human foods can be supplemented, meanwhile, copper, which can play, kills certain in running water The effect of a little bacteriums.But drink the too high water of copper content, then discomfort can be brought to body, cause and have a stomach upset, cause to vomit And diarrhoea.A large amount of intake copper can promote selenium deficiency, and disturb the absorption of iron;Feed copper excessively not only suppresses zinc-iron alloy solution, and can add Fast zinc excretion, clinic, which has, throws copper treatment copper shortage and causes the report of zinc-deficiency disease.
The detection of copper ion is more using atomic absorption spectrophotometry, visible spectrophotometry, inductive etc. in water Plasma-atomic emission spectrum/mass spectrography, electrochemical methods etc., these methods are both needed to taking back water sample into special laboratory, Chemically examined and completed by the operating technology personnel of specialty, most of method also needs to the high large-scale instrumentation of price, it is impossible to meets Quickly, easy Site Detection requirement, some special when and wheres also in the presence of sampling not in time, sampling can not be objectively responded The situation of live Water quality.
Existing copper ion Site Detection reagent is mostly the dry-type detection reagent based on colorimetric method in the market, The detection reagent takes a certain amount of water sample mix, dissolve with dry-type detection reagent when measuring, react a period of time after Specific color is produced, the concentration for drawing water sample test substance is directly compareed with standard color card.Such method is quick, conveniently, it is public Many participations are high, but influence of the change to measurement result of reagent blank can not be eliminated during measurement, only constant in reagent blank Just there is higher accuracy when constant.But the detection reagent of actually detected discovery, either liquid-type or dry-type, That reagent blank becomes larger or diminished in storing process all inevitably be present.In addition, the inspection using dry-type , it is necessary to first detection reagent is fully dissolved with water sample to be measured when test agent carries out water sample detection, then with the specific pollution in water sample Thing carries out reaction and produces particular color.Because the TWS that Various Seasonal, different zones gather is not quite similar, dry-type is dissolved Also it is not quite similar the time required to during detection reagent, is difficult to effectively control the time required to whole detection process, also to testing result Accuracy adversely affects.
Therefore, demand it is a kind of using liquid-type detection reagent, reagent blank can be eliminated testing result is influenceed, using standard The detection method of copper ion in the quantitative water of the direct visual colorimetric determination of colour atla, for realizing, copper ion field quick detection seems in water It is particularly important.
The content of the invention
The purpose of the embodiment of the present invention in a kind of water for eliminating reagent blank influence is provided copper ion scene it is quick Detection method, to realize the live quick, Accurate Determining of copper ion in water, prior art is overcome when using dry-type detection reagent When being difficult to effective control detection time because of the difference of dissolution time, not deducting standard color card, the colorimetric of reagent blank because using This standard color card is directly compareed with sample cell obtain a result, the fluctuation that can not eliminate reagent blank is made to testing result accuracy The shortcomings that into adverse effect..
The present invention is achieved in that copper ion field fast detection method in a kind of water for eliminating reagent blank influence Including:
Step 1: preparing liquid-type copper ion detection reagent, it is containing 2- (5- Bromo-2-pyridylazos) -5- diethylaminos The Acetic acid-sodium acetate buffer solution that base phenol, Tween-80, hydrogen peroxide, sodium fluoride, the pH of ethylene glycol are 3.5;
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark Quasi- pipe, the copper ion standard liquid of deionized water and various concentrations is added in blank tube and standard pipe, adds step respectively Liquid-type copper ion detection reagent described in one, reaction a period of time;
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding copper ion standard liquid Concentration be printed upon on water white transparency PVC plastic flitch, obtain deduct reagent blank after copper ion standard color card;
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample QC, deionized water and water sample to be measured are added in blank tube and sample cell respectively, adds the liquid-type described in step 1 Copper ion detection reagent, reaction a period of time;
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when blank tube is superimposed with standard color card When color afterwards is with sample cell solid colour, the copper ion concentration corresponding to standard color card, as water are read within a certain period of time Copper ion concentration in sample.
Further, in step 1, acetic acid that pH that described liquid-type copper ion detection reagent is 60mmol/L is 3.5- Sodium-acetate buffer, the concentration of the 2- contained (5- Bromo-2-pyridylazos) -5- diethylamino phenols is 0.04-0.10g/L, tween - 80 concentration is 0.02-0.08ml/L, the concentration of hydrogen peroxide is 3-10ml/L, the concentration of sodium fluoride is 5-25g/L, ethylene glycol Concentration be 10-30ml/L, compound method is:Weigh 0.04-0.10g 2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 5-25g sodium fluorides, add 800ml 60mmol/LpH3.5 Acetic acid-sodium acetate buffer solution stirring and dissolvings, add 0.02-0.08ml Tween-80,3-10ml hydrogen peroxide, 10-30ml ethylene glycol, delayed with 60mmol/L pH3.5 Acetic acid-sodium acetates after stirring and dissolving Fliud flushing is settled to 1000ml.
Further, in step 2, the concentration of described copper ion standard liquid be respectively 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L, compound method are:It is 99.99% that precision, which weighs 0.1000g contents, Metallic copper, with 1000ml is settled to after 10ml 50% nitric acid dissolving, the copper ion storing solution that concentration is 100mg/L is obtained, With deionized water by copper ion storing solution be diluted to concentration be 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L copper ion standard liquid.
Further, in step 2 and step 4, the copper ion standard liquid of described addition deionized water and various concentrations Volume be 10ml.
Further, in step 2 and step 4, the volume of described addition liquid-type copper ion detection reagent is 0.2ml.
Further, in step 2 and step 4, preferred reaction conditions are that 5-15 minutes are reacted in 5-40 DEG C of environment.
Further, in step 3, the thickness of described water white transparency PVC plastic flitch is 1-2mm.
Further, in step 5, described certain time is 5-10 minutes.
The copper ion field fast detection method for eliminating reagent blank influence of the present invention has that method is simple, accuracy The advantages of high, easily operated;Detected, can be effectively controlled needed for whole detection process using liquid-type copper ion detection reagent Time;Method by the way that blank tube color addition to be carried out to colorimetric control with sample cell again on standard color card, can effectively be eliminated Influence of the reagent blank to testing result, is particularly suitable for copper ion field quick detection in water.
Brief description of the drawings
Fig. 1 is copper ion field fast detection method in the water provided in an embodiment of the present invention for eliminating reagent blank influence Flow chart.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, book is sent out It is bright to be further elaborated.It should be appreciated that specific embodiment described herein is only to explain this present invention, not For limiting the present invention.
Below in conjunction with the accompanying drawings and specific embodiment is further described to the application principle of the present invention.
As shown in figure 1, the present invention is achieved in that in a kind of water for eliminating reagent blank influence that copper ion scene is fast Fast detection method includes:
S101, liquid-type copper ion detection reagent is prepared, it is containing 2- (5- Bromo-2-pyridylazos) -5- lignocaines The Acetic acid-sodium acetate buffer solution that phenol, Tween-80, hydrogen peroxide, sodium fluoride, the pH of ethylene glycol are 3.5;
S102,7 10ml glass colorimetric cylinders are taken, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are standard Pipe, the copper ion standard liquid of deionized water and various concentrations is added in blank tube and standard pipe respectively, adds step Liquid-type copper ion detection reagent described in S101, reaction a period of time;
S103, the color as caused by each tube reaction in the bottom up shooting step S102 of colorimetric cylinder, use mapping software After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding copper ion standard liquid Concentration be printed upon on water white transparency PVC plastic flitch, obtain deduct reagent blank after copper ion standard color card;
S104,2 10ml glass colorimetric cylinders are separately taken, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample Pipe, deionized water and water sample to be measured are added in blank tube and sample cell respectively, adds the liquid-type described in step S101 Copper ion detection reagent, reaction a period of time;
S105, the blank bottom of the tube that the standard color card described in step S103 is placed in step S104, by colorimetric bottom of the tube Look up the color after blank tube is superimposed with standard color card, and with sample cell color comparator, when blank tube and reference colour card stack When color after adding is with sample cell solid colour, the copper ion concentration corresponding to standard color card is read within a certain period of time, is Determined Copper in Aqueous Solution concentration.
Further, in step S101, vinegar that pH that described liquid-type copper ion detection reagent is 60mmol/L is 3.5 Acid-sodium-acetate buffer, the concentration of the 2- contained (5- Bromo-2-pyridylazos) -5- diethylamino phenols are 0.04-0.10g/L, told The concentration of temperature -80 is 0.02-0.08ml/L, the concentration of hydrogen peroxide is 3-10ml/L, the concentration of sodium fluoride is 5-25g/L, second The concentration of glycol is 10-30ml/L, and compound method is:Weigh 0.04-0.10g 2- (5- Bromo-2-pyridylazos) -5- diethylaminos Base phenol, 5-25g sodium fluorides, add 800ml 60mmol/L pH3.5 Acetic acid-sodium acetate buffer solution stirring and dissolvings, add 0.02- 0.08ml Tween-80s, 3-10ml hydrogen peroxide, 10-30ml ethylene glycol, 60mmol/L pH3.5 acetic acid-vinegar is used after stirring and dissolving Sour sodium buffer solution is settled to 1000ml.
Further, in step S102, the concentration of described copper ion standard liquid be respectively 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L, compound method are:It is 99.99% that precision, which weighs 0.1000g contents, Metallic copper, with 1000ml is settled to after 10ml 50% nitric acid dissolving, the copper ion storing solution that concentration is 100mg/L is obtained, With deionized water by copper ion storing solution be diluted to concentration be 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L copper ion standard liquid.
Further, in step S102 and step S104, the copper ion standard of described addition deionized water and various concentrations The volume of solution is 10ml.
Further, in step S102 and step S104, the volume of described addition liquid-type copper ion detection reagent is 0.2ml。
Further, in step S102 and step S104, preferred reaction conditions are that 5-15 minutes are reacted in 5-40 DEG C of environment.
Further, in step S103, the thickness of described water white transparency PVC plastic flitch is 1-2mm.
Further, in step S105, described certain time is 5-10 minutes.
The specific embodiment of the present invention:
Embodiment 1
Step 1: weighing 0.04g 2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 5g sodium fluorides, add 800ml60mmol/L pH3.5 Acetic acid-sodium acetate buffer solution stirring and dissolvings, add 0.02ml Tween-80s, 3ml hydrogen peroxide, 10ml ethylene glycol, 1000ml is settled to 60mmol/L pH3.5 Acetic acid-sodium acetate buffer solutions after stirring and dissolving, obtains 1000ml Contain 0.04g/L2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 0.02ml/L Tween-80s, 3ml/L hydrogen peroxide, 5g/ L sodium fluorides, the 60mmol/L pH3.5 Acetic acid-sodium acetates buffer solution of 10ml/L ethylene glycol are liquid-type copper ion detection reagent;
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark Quasi- pipe, added respectively in blank tube and standard pipe 10ml deionized waters and 10ml concentration be 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L copper ion standard liquid, add the liquid-type described in step 1 Copper ion detection reagent 0.2ml, reacted 15 minutes in 5 DEG C of environment;
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding copper ion standard liquid Concentration be printed upon thickness be 1mm water white transparency PVC plastic flitch on, obtain deduct reagent blank after each concentration copper ion mark Quasi- colour atla;
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample QC, 10ml deionized waters and 10ml water samples to be measured are added in blank tube and sample cell respectively, is added described in step 1 Liquid-type copper ion detection reagent, reacted 15 minutes in 5 DEG C of environment;
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when both solid colours, at 5 points The copper ion concentration corresponding to standard color card is read in clock, it is 0.04mg/L to draw Determined Copper in Aqueous Solution concentration.
The copper ion concentration of water sample described in the present embodiment step 4 is detected with national standard method atomic absorption method, As a result it is 0.042mg/L, it can be seen that the present embodiment methods described has consistent well with the testing result of atomic absorption method Property.
Embodiment 2
Step 1: weighing 0.07g 2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 15g sodium fluorides, add 800ml60mmol/L pH3.5 Acetic acid-sodium acetate buffer solution stirring and dissolvings, add 0.05ml Tween-80s, 6.5ml peroxidating Hydrogen, 20ml ethylene glycol, 1000ml is settled to 60mmol/L pH3.5 Acetic acid-sodium acetate buffer solutions after stirring and dissolving, is obtained 1000ml contains 0.07g/L2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 0.05ml/L Tween-80s, 6.5ml/L peroxides It is liquid-type copper ion to change hydrogen, 15g/L sodium fluorides, the 60mmol/L pH3.5 Acetic acid-sodium acetates buffer solution of 20ml/L ethylene glycol Detection reagent;
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark Quasi- pipe, added respectively in blank tube and standard pipe 10ml deionized waters and 10ml concentration be 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L copper ion standard liquid, add the liquid-type described in step 1 Copper ion detection reagent 0.2ml, reacted 10 minutes in 20 DEG C of environment;
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding copper ion standard liquid Concentration be printed upon thickness be 1.5mm water white transparency PVC plastic flitch on, obtain deduct reagent blank after each concentration copper ion Standard color card;
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample QC, 10ml deionized waters and 10ml water samples to be measured are added in blank tube and sample cell respectively, is added described in step 1 Liquid-type copper ion detection reagent, reacted 10 minutes in 20 DEG C of environment;
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when both solid colours, 7.5 The copper ion concentration corresponding to standard color card is read in minute, it is 0.12mg/L to draw Determined Copper in Aqueous Solution concentration.
The copper ion concentration of water sample described in the present embodiment step 4 is detected with national standard method atomic absorption method, As a result it is 0.124mg/L, it can be seen that the present embodiment methods described has consistent well with the testing result of atomic absorption method Property.
Embodiment 3
Step 1: weighing 0.10g 2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 25g sodium fluorides, add 800ml60mmol/L pH3.5 Acetic acid-sodium acetate buffer solution stirring and dissolvings, add 0.08ml Tween-80s, 10ml peroxidating Hydrogen, 30ml ethylene glycol, 1000ml is settled to 60mmol/L pH3.5 Acetic acid-sodium acetate buffer solutions after stirring and dissolving, is obtained 1000ml contains 0.10g/L2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 0.08ml/L Tween-80s, 10ml/L peroxides It is liquid-type copper ion to change hydrogen, 25g/L sodium fluorides, the 60mmol/L pH3.5 Acetic acid-sodium acetates buffer solution of 30ml/L ethylene glycol Detection reagent;
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark Quasi- pipe, added respectively in blank tube and standard pipe 10ml deionized waters and 10ml concentration be 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L copper ion standard liquid, add the liquid-type described in step 1 Copper ion detection reagent 0.2ml, reacted 5 minutes in 40 DEG C of environment;
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding copper ion standard liquid Concentration be printed upon thickness be 2mm water white transparency PVC plastic flitch on, obtain deduct reagent blank after each concentration copper ion mark Quasi- colour atla;
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample QC, 10ml deionized waters and 10ml water samples to be measured are added in blank tube and sample cell respectively, is added described in step 1 Liquid-type copper ion detection reagent, reacted 5 minutes in 40 DEG C of environment;
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when both solid colours, at 10 points The copper ion concentration corresponding to standard color card is read in clock, it is 0.24mg/L to draw Determined Copper in Aqueous Solution concentration.
The copper ion concentration of water sample described in the present embodiment step 4 is detected with national standard method atomic absorption method, As a result it is 0.236mg/L, it can be seen that the present embodiment methods described has consistent well with the testing result of atomic absorption method Property.
Embodiment 4
In order to further prove the creativeness of the present invention, the present invention has done following contrast test, has investigated the change of reagent blank Change the influence to measurement result accuracy.Method as described in the step 1 of this law embodiment 1 prepares the detection examination of liquid-type copper ion Agent is test group, not deduct reagent blank, use the detection reagent of standard color card direct color comparison as control group, by two group reagents 4 DEG C of refrigerators are put respectively and 37 water-baths store 7 days, and to being with aas determination copper ion concentration with portion 0.04mg/L water sample is measured, while replaces water sample to carry out reagent blank test with deionized water, with 721 spectrophotometers Reagent blank value is recorded, cuvette optical path is 1cm during test, and test wavelength 560nm, reference solution is deionized water, as a result It is shown in Table 1.
Table 1
As it can be seen from table 1 under identical experiment condition, with the gradual increase of reagent blank, the measure of test group As a result keep constant, but the measurement result of control group substantially becomes big, shows that method of the present invention can effectively remove reagent sky Influence of the white change to copper ion test result.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.

Claims (10)

1. copper ion field fast detection method in a kind of water for eliminating reagent blank influence, it is characterised in that described can Eliminating copper ion field fast detection method in the water that reagent blank influences includes:Step 1: prepare the inspection of liquid-type copper ion Test agent, it is containing 2- (the bromo- 2- pyridylazos of 5-) -5- diethylamino phenols, Tween-80, hydrogen peroxide, fluorination Acetic acid-sodium-acetate buffer that sodium, the pH of ethylene glycol are 3.5;Step 2: 7 10ml glass colorimetric cylinders are taken, To set the 1st colorimetric cylinder be blank tube, 2-7 root colorimetric cylinders are standard pipe, is added respectively in blank tube and standard pipe The copper ion standard liquid of deionized water and various concentrations, the liquid-type copper ion detection reagent described in step 1 is added, is entered Row reaction;Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software It is after each standard pipe color is deducted blank tube color by photoshop7.0, the color of gained and corresponding copper ion standard is molten The concentration of liquid is printed upon on water white transparency PVC plastic plates, obtains deducting the copper ion standard color card after reagent blank;Step 4th, another to take 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample cell, Deionized water and water sample to be measured are added in blank tube and sample cell respectively, adds the liquid-type copper ion described in step 1 Detection reagent, reacted;Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, by The bottom up observation blank tube of colorimetric cylinder be superimposed with standard color card after color, and with sample cell color comparator, when blank tube and During color and sample cell solid colour after standard color card superposition, the copper ion concentration corresponding to standard color card, as water are read Copper ion concentration in sample.
2. copper ion field fast detection method in the water for eliminating reagent blank influence as described in claim 1, it is special Sign is, in step 1, acetic acid that pH that described liquid-type copper ion detection reagent is 60mmol/L is 3.5- Sodium-acetate buffer, the concentration of the 2- contained (the bromo- 2- pyridylazos of 5-) -5- diethylamino phenols is 0.04-0.10g/ L, the concentration of Tween-80 is 0.02-0.08ml/L, the concentration of hydrogen peroxide is 3-10ml/L, the concentration of sodium fluoride is 5- 25g/L, the concentration of ethylene glycol are 10-30ml/L.
3. copper ion field fast detection method in the water for eliminating reagent blank influence as described in claim 2, it is special Sign is that acetic acid-sodium acetate buffer liquid making method is:Weigh 0.04-0.10g 2- (the bromo- 2- pyridylazos of 5- ) -5- diethylamino phenols, 5-25g sodium fluorides, add the stirring of 800ml 60mmol/L pH3.5 acetic acid-sodium-acetate buffer Dissolving, adds 0.02-0.08ml Tween-80s, 3-10ml hydrogen peroxide, 10-30ml ethylene glycol, is used after stirring and dissolving 60mmol/L pH3.5 acetic acid-sodium-acetate buffer is settled to 1000ml.
4. copper ion field fast detection method in the water for eliminating reagent blank influence as described in claim 1, it is special Sign is, in step 2, the concentration of described copper ion standard liquid be respectively 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L、0.20mg/L、0.24mg/L。
5. copper ion field fast detection method in the water for eliminating reagent blank influence as described in claim 4, it is special Sign is that copper ion standard liquid compound method is:The metallic copper that 0.1000g contents are 99.99% is weighed, uses 10ml 1000ml is settled to after 50% nitric acid dissolving, concentration is obtained and is 100mg/L copper ion storing solution, then use deionized water By copper ion storing solution be diluted to concentration for 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L copper ion standard liquid.
6. copper ion field fast detection method in the water for eliminating reagent blank influence as described in claim 1, it is special Sign is, in step 2, the volume of the copper ion standard liquid of described addition deionized water and various concentrations is 10ml, step In rapid four, the volume of described addition deionized water is 10ml.
7. copper ion field fast detection method in the water for eliminating reagent blank influence as described in claim 1, it is special Sign is, in step 2 and step 4, the volume of described addition liquid-type copper ion detection reagent is 0.2ml.
8. copper ion field fast detection method in the water for eliminating reagent blank influence as described in claim 1, it is special Sign is, in step 2 and step 4, reaction condition is that 5-15 minutes are reacted in 5-40 DEG C of environment.
9. copper ion field fast detection method in the water for eliminating reagent blank influence as described in claim 1, it is special Sign is, in step 3, the thickness of described water white transparency PVC plastic plates is 1-2mm.
10. copper ion field fast detection method in the water for eliminating reagent blank influence as described in claim 1, it is special Sign is, the copper ion concentration corresponding to standard color card is read in step 5, after reaction 5-10 minutes.
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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1456876A (en) * 2003-03-14 2003-11-19 中国人民解放军军事医学科学院卫生学环境医学研究所 Copper detecting paper and preparing method thereof
CN1635379A (en) * 2004-11-30 2005-07-06 曹建明 Serum copper, iron and zinc rapid sensitive detection kit and preparing method thereof
CN101097220A (en) * 2006-06-28 2008-01-02 温州医学院 Blood serum copper, zincium and iron 5-br-padcap even phase automatic analysis method and liquid stabilizing reagent
CN101281139A (en) * 2008-05-13 2008-10-08 北京大学 Copper ion test paper and preparation thereof
CN101995383A (en) * 2010-03-31 2011-03-30 中国商用飞机有限责任公司 Method for measuring copper ion content of solution
CN102095724A (en) * 2010-11-24 2011-06-15 青岛佳明测控仪器有限公司 Method for determining copper content in water by biquinolyl spectrophotometry
CN102818804A (en) * 2012-08-09 2012-12-12 中国科学院唐山高新技术研究与转化中心 Kit and detection method for determining copper ion concentration in water environment
CN103278497A (en) * 2013-05-15 2013-09-04 中国科学院烟台海岸带研究所 Copper ion colorimetry detecting kit and detecting method thereof
CN103335966A (en) * 2013-06-20 2013-10-02 工信华鑫科技有限公司 Detection method for trace copper in solution
JP5360707B2 (en) * 2006-02-23 2013-12-04 株式会社シノテスト Metal colorimetric measurement method and reagent

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6069552A (en) * 1983-09-26 1985-04-20 Wako Pure Chem Ind Ltd Method and reagent for colorimetric determination of copper
JPH0599928A (en) * 1991-10-07 1993-04-23 Fuji Photo Film Co Ltd Reagent composition for detection of copper ion in urine

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1456876A (en) * 2003-03-14 2003-11-19 中国人民解放军军事医学科学院卫生学环境医学研究所 Copper detecting paper and preparing method thereof
CN1635379A (en) * 2004-11-30 2005-07-06 曹建明 Serum copper, iron and zinc rapid sensitive detection kit and preparing method thereof
JP5360707B2 (en) * 2006-02-23 2013-12-04 株式会社シノテスト Metal colorimetric measurement method and reagent
CN101097220A (en) * 2006-06-28 2008-01-02 温州医学院 Blood serum copper, zincium and iron 5-br-padcap even phase automatic analysis method and liquid stabilizing reagent
CN101281139A (en) * 2008-05-13 2008-10-08 北京大学 Copper ion test paper and preparation thereof
CN101995383A (en) * 2010-03-31 2011-03-30 中国商用飞机有限责任公司 Method for measuring copper ion content of solution
CN102095724A (en) * 2010-11-24 2011-06-15 青岛佳明测控仪器有限公司 Method for determining copper content in water by biquinolyl spectrophotometry
CN102818804A (en) * 2012-08-09 2012-12-12 中国科学院唐山高新技术研究与转化中心 Kit and detection method for determining copper ion concentration in water environment
CN103278497A (en) * 2013-05-15 2013-09-04 中国科学院烟台海岸带研究所 Copper ion colorimetry detecting kit and detecting method thereof
CN103335966A (en) * 2013-06-20 2013-10-02 工信华鑫科技有限公司 Detection method for trace copper in solution

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
5-Br-PADAP 光度法同时测定电镀 CIS薄膜废液中的铜和铟;宋小杰 等;《应用化工》;20120131;第41卷(第1期);第174-176、182页 *
杂环偶氮类显色剂在生物样品铜、锌、铁光度测定法中的应用;连国军 等;《广东微量元素科学》;20051231;第12卷(第12期);第17-22页 *

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