CN105241876A - Detection method of sulfur dioxide in wine - Google Patents
Detection method of sulfur dioxide in wine Download PDFInfo
- Publication number
- CN105241876A CN105241876A CN201510650231.1A CN201510650231A CN105241876A CN 105241876 A CN105241876 A CN 105241876A CN 201510650231 A CN201510650231 A CN 201510650231A CN 105241876 A CN105241876 A CN 105241876A
- Authority
- CN
- China
- Prior art keywords
- solution
- sample
- sulphuric dioxide
- detection method
- standard
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention provides a detection method of sulfur dioxide in wine, wherein the method includes the following steps: firstly, using a sulfur dioxide standard stock solution, adding a formaldehyde buffer absorption liquid, and preparing a series of standard solutions having the sulfur dioxide content of 0-50 [mu]g; then diluting a sample, carrying out decoloring purification treatment by an adsorbent, then adding the formaldehyde buffer absorption liquid, and stabilizing; next adding a sodium sulphamidate solution and a sodium hydroxide solution to the series of prepared sulfur dioxide standard solutions and the sample solution, mixing evenly, then adding a pararosaniline hydrochloride solution, and developing for 5-40 minutes at the temperature of 10-30 DEG C; and finally, determining the absorbance of the sulfur dioxide standard solutions and the absorbance of the sample by a food fast detector, drawing a standard curve according to the absorbance and the content of the sulfur dioxide standard solutions, and then calculating to obtain the content of sulfur dioxide in the sample according to the standard curve. The detection method rapidly detects sulfur dioxide in the grape wine especially dark grape wine, and the operation method is quite simple.
Description
Technical field
The present invention relates to a kind of detection method, particularly relate to the detection method of sulphuric dioxide in wine, belong to detection analysis field.
Background technology
In food production process, manufacturer often adds the variable color factor that bleaching agent etc. destroys food, keeps food to avoid brown stain occurs, thus keeps the peculiar gloss of food and control its component.And in China's food service industry, use maximum component for sulphuric dioxide and sulphite, in recent years, because the situation of the Use out of range of sulphite in foods and abuse is day by day serious, directly cause sulfur dioxide content in food obviously to increase.In addition, also there is part illegal product man, in food, directly add a large amount of sulphuric dioxide.
Sulfur dioxide residual quantity is the metering form that sulphite exists in food, and sulphite mainly comprises the sulphuric dioxide etc. that sodium sulphite, sodium bisulfite, Hydros (having another name called sodium hydrosulfite), sodium pyrosulfite, potassium metabisulfite and sulfur burning generate.These materials are dissociated into the sulphurous acid with strong reducing property in food, play bleaching, decolouring, anticorrosion and antioxidation.But sulphite has certain toxicity, reversible reaction can be carried out with the sulfydryl of protein, stimulate digestion mucous membrane, there is the symptoms such as nausea,vomiting,diarrhea. the sulphite of excess intake, the absorption of human body to calcium can be affected, and destroying B family vitamin, long-term absorption then can cause damage to liver.FDA (FDA) requires to be indicated the food of sulphite use amount higher than 10mg/kg.There is strict limit standard in China's " food additives use hygienic standard " to the residual quantity that the sulphuric dioxide in food and sulphite etc. allow, but the phenomenon that current sulfur dioxide in food and sulphite etc. exceed standard is still more general.
Grape wine take grape as the fermented wine, particularly extra dry red wine of raw material, because having certain nutritive value, and dark liking by domestic consumer.Import volume vinous also increases year by year.In wine production process, sulphite is widely used as antiseptic, owing to not finding better adjuvant substitute at present, so as a part of preparing burden, in grape wine, comprise the sulfur dioxide residue that import grape wine has different content level.Equally, the sulphuric dioxide in grape wine is too high also can bring harm to consumer.State compulsory standard GB15037-2006 " grape wine " has also formulated the limit standard of 250mg/L (in sulphuric dioxide).
The detection method of sulfite content is a lot, and conventional has: pararosaniline hydrochloridc colourimetry, distillation-iodometry, direct titrimetric method etc.But colourimetry, for itself having red or sample rosy (as claret), can produce obvious interference, and be difficult to deduction; Direct titrimetric method, also causes the inaccurate of measurement result because peony vinous affects the judgement of titration end-point.So, standard GB/T/T15038-2006 " grape wine, fruit wine GENERAL EXPERIMENTATION " defines distillation-iodometry to measure the sulphite in grape wine, but the method approximately need distill more than 1 hour, consuming time longer, and mass detection speed is too slow.When large sample amount, such as import grape wine in port detects, and the method obviously can not meet fast and convenient detection demand.
Summary of the invention
The object of the present invention is to provide the detection method of sulphuric dioxide in a kind of wine, for overcoming, prior art process is loaded down with trivial details, detection time is long, the high deficiency of operation requirements, set up the method for quick of sulphuric dioxide in a kind of grape wine, convenient, fast, to complete in grape wine content of sulfur dioxide accurately and efficiently and detect.
For solving the problems of the technologies described above, technical scheme provided by the invention is as follows:
In wine, the detection method of sulphuric dioxide, is characterized in that, sample, after adsorbent purification and decoloration, carries out content detection by pararosaniline hydrochloridc colourimetry.
Preferably, described detection method comprises the steps:
(1) sulphuric dioxide standard solution preparation: utilize sulphuric dioxide standard stock solution, add buffered formaldehyde absorbing liquid and be configured to the series standard solution that content of sulfur dioxide is 0-50 μ g;
(2) sample desolventing technology: the described sample accurately pipetting 0.2-0.5ml, doubly, then sample thief dilution, through adsorbent purification and decoloration, gets filtrate and adds buffered formaldehyde absorbing liquid and stablize dilute with water 10-50, obtains sample solution;
(3) chromogenic reaction: in the sample solution handled well in the serial sulphuric dioxide standard solution prepared in step (1) and step (2), add dithiocarbamic acid sodium water solution and sodium hydrate aqueous solution respectively, mixing, then pararosaniline hydrochloridc aqueous solution is added, at 10-30 DEG C, react 5-40 minute, obtain corresponding serial sulphuric dioxide standard coloration liquid and sample nitrite ion;
(4) detect: after in step (3), chromogenic reaction completes, the serial sulphuric dioxide standard coloration liquid utilizing food science literature instrument to obtain step (3) at wavelength 577nm place and sample nitrite ion carry out absorbance measurement respectively, according to absorbance and the concentration drawing standard curve of serial sulphuric dioxide standard coloration liquid, then the absorbance reference standard curve of nitrite ion calculates the content of sulphuric dioxide in sample solution per sample.
Preferably, the volume of described serial sulphuric dioxide normal concentration solution and described sample solution is 5mL, and the addition of described dithiocarbamic acid sodium water solution and sodium hydrate aqueous solution is 5, and the addition of described pararosaniline hydrochloridc aqueous solution is 0.5mL.
Preferably, described adsorbent is strong cation-exchanging resin, carbon nano-tube.
Preferably, the solid phase extraction column of described adsorbent made by the sorbing material such as strong cation-exchanging resin, carbon nano-tube.
Preferably, described adsorbent is WatersMCX pillar or carbon nano-tube.
Preferably, the collocation method of described buffered formaldehyde absorbing liquid is: be the formalin 3-6mL of 36% ~ 38%, 1,2-diaminocyclohexane tetraacetic acid two sodium water solution 10-25mL and the Potassium Hydrogen Phthalate aqueous solution containing 1.5-2.5g Potassium Hydrogen Phthalate by volumetric concentration, be diluted with water to 100mL again, obtain buffered formaldehyde and absorb stock solution, and then with water, buffered formaldehyde is absorbed stock solution and dilute 10 times, namely obtain described buffered formaldehyde absorbing liquid.
Preferably, the concentration of 1,2-diaminocyclohexane tetraacetic acid two sodium water solution of the configuration use of described buffered formaldehyde absorbing liquid is 0.05mol/L.
Preferably, the mass concentration of described dithiocarbamic acid sodium water solution is 4.0-6.0g/L, and the concentration of described sodium hydrate aqueous solution is 0.5-1.5mol/L.
Preferably, the mass concentration of described pararosaniline hydrochloridc aqueous solution is 0.3-0.6g/L, collocation method is: drawing 15-30mL mass concentration is that the Pararosaniline stock solution of 2.0g/L is in 100mL volumetric flask, adding 25-35mL volumetric concentration is the strong phosphoric acid of 85% and the 12mol/L concentrated hydrochloric acid of 8-16mL, be diluted with water to graticule, shake up, place after spending the night and use.
Preferably, described food science literature instrument is portable tintmeter or spectrophotometer.
Compared with prior art, in wine provided by the invention, the advantage of the detection method of sulphuric dioxide is:
1. utilize this method to achieve the grape wine particularly quick detection of sulphuric dioxide in dark grape wine, compared with the existing way of distillation, detection time can be shortened greatly.In prior art, when adopting the way of distillation to detect sample, need to carry out distillation process to sample, to get rid of the interference of pigment in sample, single sample at least needs 45 minutes, and single covering device completes 10 samples at least 6 hours; And the present invention adopts adsorbent to carry out purification and decoloration process to sample, single sample can complete detection within half an hour, and single covering device at least can complete the detection of 10 samples in 1 hour;
2. this law equipment therefor is mancarried device, without the need to alternating current after charging, can complete detection in laboratory or scene; And the way of distillation needs complicated glass distilling apparatus, detection can only be completed in laboratory.
3. this law is simple to operate, and has commercial kit to sell, and can complete detection, improve detection efficiency through simple training; And the analytical procedures such as the preparation of the heating of way of distillation action need, distillation, related reagent, operate complicated, need the personnel of certain chemical analysis level just can complete.
Embodiment
In the wine proposed the present invention below in conjunction with specific embodiment, the detection method of sulphuric dioxide is described in further detail.According to the following describes and claims, advantages and features of the invention will be clearer.
Embodiment 1: the detection method of sulphuric dioxide in wine
Experiment agents useful for same and sample are commercial commodity.
A detection method for sulphuric dioxide in wine, sample, after adsorbent purification and decoloration, carries out content detection by pararosaniline hydrochloridc colourimetry.Concrete steps comprise:
(1) serial sulphuric dioxide normal concentration solution preparation:
A) sulphuric dioxide standard solution: compound concentration is the sulphuric dioxide mark liquid of 10.0 μ g/mL.With buffered formaldehyde absorbing liquid, commercially available sulphuric dioxide standard solution is diluted to every milliliter of calibration solution containing 10.0 μ g sulphuric dioxide.This solution, for drawing calibration curve, refrigerates, Absorbable organic halogens 1 month at 4 ~ 5 DEG C.
B) pipette sulphuric dioxide standard solution 0mL respectively, 0.5mL, 1.00mL, 2.00mL, 3.00mL, 5.00mL, in 15mL graduated centrifuge tube, are settled to 5.0mL with water, then in each pipe, add 0.5mL buffered formaldehyde absorbing liquid respectively.In corresponding sulphuric dioxide standard solution series, the content range of sulphuric dioxide is 0-50 μ g.
(2) sample pre-treatments: quantitatively draw wine samples and dilute, for the grape wine that color is darker, utilize adsorbent to carry out purification and decoloration process to sample.
A) the wine samples pre-treatment that the color such as red or pink is darker:
By sulphuric dioxide different content in sample, accurately pipette 0.2mL ~ 0.5mL sample, be diluted with water to 10.0mL.Accurately pipette 0.5mL sample diluting liquid and (corresponding dilution sampling amount can be determined according to preliminary survey concentration, if preliminary survey concentration is lower, then sampling amount should be strengthened), carry out after purification and decoloration process through adsorbent, collect filtrate in centrifuge tube, be settled to 5.0mL with water, then add 0.5mL buffered formaldehyde absorbing liquid.
B) white etc. light color or colourless wine samples pre-treatment:
By sulphuric dioxide different content in sample, accurately pipette 0.2mL ~ 0.5mL sample, be diluted with water to 10.0mL.Accurately pipette 0.5mL sample diluting liquid (corresponding dilution sampling amount can be determined according to preliminary survey concentration, if preliminary survey concentration is lower, then should strengthen sampling amount), be settled to 5.0mL with water, then add 0.5mL buffered formaldehyde absorbing liquid.
(3) chromogenic reaction: carry out chromogenic reaction to the above-mentioned serial sulphuric dioxide normal concentration solution that configures and sample, concrete chromogenic reaction step is:
In above-mentioned serial sulphuric dioxide normal concentration solution and sample solution, add 5 dithiocarbamic acid sodium solutions and 5 sodium hydroxide solutions, mixing, adds 0.5mL pararosaniline hydrochloridc solution (PRA solution), at 10-30 DEG C, reacts 5-40min.
(4) detect: after chromogenic reaction completes, absorbance measurement is carried out to machine on serial sulphuric dioxide normal concentration solution and sample solution, operates according to following steps:
A) Specification Curve of Increasing: utilize food science literature instrument to detect serial sulphuric dioxide normal concentration solution, at wavelength 577nm place, with fibre-optical probe, take water as reference measurement absorbance, with the absorbance of sulphuric dioxide normal concentration solution conduit each after blank correction for ordinate, with the content of sulphuric dioxide (μ g) for horizontal ordinate, the equation of linear regression of Criterion curve.
B) in sample, content of sulfur dioxide measures: utilize food science literature instrument, at wavelength 577nm place, with fibre-optical probe, take water as reference measurement absorbance, measure with the absorbance of sample solution after blank correction, according to content of sulfur dioxide corresponding in typical curve calculation sample.
(5) result calculates:
In sample, total sulfur dioxide content obtains according to following formulae discovery:
In formula:
ρ (SO
2)---the mass concentration of sulphuric dioxide in grape wine, unit is milligrams per liter, mg/L;
M---according to the quality of sulphuric dioxide in the sample that typical curve calculates, unit μ g;
V
1---grape wine sample volume, Unit/mL;
V
2---constant volume after wine samples dilution, Unit/mL;
V
3---grape wine dilution detects volume, Unit/mL.
Further, described adsorbent is strong cation-exchanging resin or carbon nano-tube.
Further, described adsorbent is the solid phase extraction column that the sorbing material such as strong cation-exchanging resin, carbon nano-tube is made.
Further, described adsorbent is WatersMCX pillar, carbon nano-tube or pillar of the same type.
Further, the collocation method of described buffered formaldehyde absorbing liquid is: be the formalin 3-6mL of 36% ~ 38%, 1,2-diaminocyclohexane tetraacetic acid two sodium water solution 10-25mL and the Potassium Hydrogen Phthalate aqueous solution containing 1.5-2.5g Potassium Hydrogen Phthalate by volumetric concentration, be diluted with water to 100mL again, obtain buffered formaldehyde and absorb stock solution, this stock solution can preserve 1 year in refrigerator, then 10 times are diluted buffered formaldehyde being absorbed stock solution with water, namely obtain described buffered formaldehyde absorbing liquid, this damping fluid needs now with the current.
Further, the concentration of described CDTA-2Na solution is 0.05mol/L, and compound method is: take 1.82g trans 1,2-1,2-diaminocyclohexane tetraacetic acid (CDTA), add sodium hydroxide solution 6.5mL, be diluted with water to 100mL, namely obtain the CDTA-2Na solution of described 0.05mol/L.
Further, described dithiocarbamic acid sodium solution (NaH
2nSO
3) mass concentration be 4.0-6.0g/L, compound method is: take 0.36g NaOH, is placed in 100mL beaker, uses water-soluble solution, is diluted to 100mL, and then takes 0.4-0.60g dithiocarbamic acid (H
2nSO
3h), add in above-mentioned sodium hydroxide solution, be stirred to glass bar and dissolve completely.
Further, the concentration of described sodium hydroxide solution is 0.5-1.5mol/L, and compound method is: take 2-6.0gNaOH, is dissolved in 100mL water.
Further, described PRA solution is pararosaniline hydrochloridc solution, mass concentration is 0.3-0.6g/L, collocation method is: drawing 15-30mL mass concentration is that the Pararosaniline stock solution of 2.0g/L is in 100mL volumetric flask, adding 25-35mL volumetric concentration is the strong phosphoric acid of 85% and the 12mol/L concentrated hydrochloric acid of 8-16mL, is diluted with water to graticule, shakes up, placement uses after spending the night, and solution needs lucifuge to seal preservation.
Further, described food science literature instrument is portable tintmeter or spectrophotometer.
Further, in the present embodiment when carrying out sample and measuring, also carried out blank test, namely except not adding except sample, other steps are all carried out according to the experimental procedure of sample determination.
Embodiment 2: the selection of adsorbent in embodiment 1:
The preferred strong cation-exchanging resin of the present invention, carbon nano-tube carry out the adsorbent of purification and decoloration to sample as the present invention, and the adsorbent of the carried out purification and decoloration of itself and other common several models is carried out contrast test, and result is as shown in table 1.
The decolouring of the different adsorbent of table 1 and sulphite recovery result (n=3)
Result according to table 1 shows, although activated charcoal and polyamide are usually used in the Transformatin of pigment in sample, decolorizing effect is also fine, but these two kinds of adsorbents are also obvious to the absorption of sulphite, particularly activated charcoal, the ability of absorption sulphite is very strong, therefore these two kinds of adsorbents are all not suitable for the desolventing technology of sample in the present invention, and three kinds of strong cation-exchanging resins and carbon nano-tube are while decolouring, also sulphite is not adsorbed, respond well, all can be used for the pre-treatment decolorization of sample in the present invention.
Embodiment 3: the detectability of the detection method in embodiment 1 and mark-on reclaim and Precision Experiment
(1) linear relationship of detection method and detectability
According to the method described in embodiment 1, the quality taking sulphuric dioxide is respectively 0 μ g, 0.5 μ g, 1.0 μ g, 2.0 μ g, 3.0 μ g, 5.0 μ g totally 6 standard series, be configured to standard solution measure, take absorbance as ordinate, with corresponding quality for horizontal ordinate is mapped, obtain linear equation: y=0.0725x-0.004, related coefficient 0.999.Result shows, sulphuric dioxide, within the scope of 0.5-5.0 μ g, has good linear relationship between absorbance response and sample concentration.
The quantitative detection of this method is limited to 60mg/L, and in China's grape wine, sulphuric dioxide limitation is 250mg/L, can meet actual detection demand completely.
(2) mark-on reclaims and precision test
According to the method described in embodiment 1, the checking matrix that the grape wine of, extra dry white wine pink using extra dry red wine, dry type, half-dried pink, pink foaming and sweet white wine six type is tested as mark-on.
Draw 1.0mL above-mentioned sample respectively, calculate according to final constant volume, add that concentration is 0, the sulphuric dioxide standard working solution of 60mg/L, 150mg/L, 300mg/L, 400mg/L, each concentration does 6 Duplicate Samples.Empirically determination step operation in the method for room, typical curve standard measure, calculates the content of sulphuric dioxide in each sample, and sample population standard deviation and relative standard deviation, the results are shown in Table 2.
Recovery of standard addition and the precision test data (n=6) of sulphuric dioxide are added in the grape wine matrix of table 2 six type
Result according to upper table, using six kinds of grape wine as add matrix, when interpolation concentration level is 60mg/L, 150mg/L, 300mg/L, 400mg/L, the scope of average recovery of standard addition is: 82.0%-114.4%, and relative standard deviation (RSD) can be controlled within 8%.
This method is simple to operate, and measurement result accurately and reliably, is suitable as the method for quick of content of sulfur dioxide in grape wine.
Foregoing description is only the description to present pre-ferred embodiments, any restriction not to the scope of the invention, and any change that the those of ordinary skill in field of the present invention does according to above-mentioned disclosure, modification, all belong to the protection domain of claims.
Claims (10)
1. the detection method of sulphuric dioxide in wine, it is characterized in that, sample, after adsorbent purification and decoloration, carries out content detection by pararosaniline hydrochloridc colourimetry.
2. the detection method of sulphuric dioxide in wine according to claim 1, is characterized in that, comprise the steps:
(1) sulphuric dioxide standard solution preparation: utilize sulphuric dioxide standard stock solution, add buffered formaldehyde absorbing liquid and be configured to the series standard solution that content of sulfur dioxide is 0-50 μ g;
(2) sample decolorizing purification process: the described sample accurately pipetting 0.2-0.5ml, doubly, then sample thief dilution, through adsorbent purification and decoloration, gets scavenging solution and adds buffered formaldehyde absorbing liquid and stablize dilute with water 10-50, obtains sample solution;
(3) chromogenic reaction: in the sample solution handled well in the serial sulphuric dioxide normal concentration solution prepared in step (1) and step (2), add dithiocarbamic acid sodium water solution and sodium hydrate aqueous solution respectively, mixing, then pararosaniline hydrochloridc aqueous solution is added, at 10-30 DEG C, react colour developing in 5-40 minute, obtain corresponding serial sulphuric dioxide standard coloration liquid and sample nitrite ion;
(4) detect: after in step (3), chromogenic reaction completes, the serial sulphuric dioxide standard coloration liquid utilizing food science literature instrument to obtain step (3) at wavelength 577nm place and sample nitrite ion carry out absorbance measurement respectively, according to absorbance and the concentration drawing standard curve of serial sulphuric dioxide standard coloration liquid, then the absorbance reference standard curve of nitrite ion calculates the content of sulphuric dioxide in sample solution per sample.
3. the detection method of sulphuric dioxide in wine according to claim 2, it is characterized in that, the volume of described serial sulphuric dioxide normal concentration solution and described sample solution is 5mL, the addition of described dithiocarbamic acid sodium water solution and sodium hydrate aqueous solution is 5, and the addition of described pararosaniline hydrochloridc aqueous solution is 0.5mL.
4. the detection method of sulphuric dioxide in wine according to claim 1, it is characterized in that, described adsorbent is strong cation-exchanging resin or carbon nano-tube.
5. the detection method of sulphuric dioxide in wine according to claim 4, is characterized in that, the solid phase extraction column of described adsorbent made by strong cation-exchanging resin or carbon nano-tube.
6. the detection method of sulphuric dioxide in wine according to claim 2, it is characterized in that, the collocation method of described buffered formaldehyde absorbing liquid is: be the formalin 3 ~ 6mL of 36% ~ 38%, 1,2-diaminocyclohexane tetraacetic acid two sodium water solution 10 ~ 25mL and the Potassium Hydrogen Phthalate aqueous solution containing 1.5 ~ 2.5g Potassium Hydrogen Phthalate by volumetric concentration, be diluted with water to 100mL again, obtain buffered formaldehyde and absorb stock solution, and then with water, buffered formaldehyde is absorbed stock solution and dilute 10 times, namely obtain described buffered formaldehyde absorbing liquid.
7. the detection method of sulphuric dioxide in wine according to claim 6, it is characterized in that, the concentration of described 1,2-diaminocyclohexane tetraacetic acid two sodium water solution is 0.05mol/L.
8. the detection method of sulphuric dioxide in wine according to claim 2, it is characterized in that, the mass concentration of described dithiocarbamic acid sodium water solution is 4.0-6.0g/L, and the concentration of described sodium hydrate aqueous solution is 0.5-1.5mol/L.
9. the detection method of sulphuric dioxide in wine according to claim 2, it is characterized in that, the mass concentration of described pararosaniline hydrochloridc aqueous solution is 0.3-0.6g/L, collocation method is: drawing 15-30mL mass concentration is that the Pararosaniline stock solution of 2.0g/L is in 100mL volumetric flask, adding 25-35mL volumetric concentration is the strong phosphoric acid of 85% and the 12mol/L concentrated hydrochloric acid of 8-16mL, be diluted with water to graticule, shake up, place after spending the night and use.
10. the detection method of sulphuric dioxide in wine according to claim 2, it is characterized in that, described food science literature instrument is portable tintmeter or spectrophotometer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510650231.1A CN105241876A (en) | 2015-10-09 | 2015-10-09 | Detection method of sulfur dioxide in wine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510650231.1A CN105241876A (en) | 2015-10-09 | 2015-10-09 | Detection method of sulfur dioxide in wine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105241876A true CN105241876A (en) | 2016-01-13 |
Family
ID=55039599
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510650231.1A Pending CN105241876A (en) | 2015-10-09 | 2015-10-09 | Detection method of sulfur dioxide in wine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105241876A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107462575A (en) * | 2017-07-25 | 2017-12-12 | 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） | A kind of sulfur dioxide quick detection kit and detection method |
| CN111257502A (en) * | 2020-03-05 | 2020-06-09 | 江苏权正检验检测有限公司 | Method for measuring sulfur dioxide in dried mushrooms |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003075423A (en) * | 2001-09-06 | 2003-03-12 | Shimadzu Corp | Method for analyzing liberation sulfurous acid and total sulfurous acid |
| CN101865830A (en) * | 2009-04-17 | 2010-10-20 | 桑华春 | Method for detecting sulfur dioxide in food |
| US20150000371A1 (en) * | 2013-05-07 | 2015-01-01 | Russell W. Greene | Beverage diagnostic and preservation devices and methods |
-
2015
- 2015-10-09 CN CN201510650231.1A patent/CN105241876A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003075423A (en) * | 2001-09-06 | 2003-03-12 | Shimadzu Corp | Method for analyzing liberation sulfurous acid and total sulfurous acid |
| CN101865830A (en) * | 2009-04-17 | 2010-10-20 | 桑华春 | Method for detecting sulfur dioxide in food |
| US20150000371A1 (en) * | 2013-05-07 | 2015-01-01 | Russell W. Greene | Beverage diagnostic and preservation devices and methods |
Non-Patent Citations (4)
| Title |
|---|
| 国家环境保护总局: "《空气和废气监测分析方法》", 30 September 2003 * |
| 李凤: "脱色对红葡萄酒中还原糖测定的影响", 《安徽农业科学》 * |
| 杨文英等: "甲醛吸收- 盐酸副玫瑰苯胺法测定食品中的亚硫酸盐", 《中国卫生检验杂志》 * |
| 郎海: "无汞吸收—盐酸副玫瑰苯胺比色法测定食品中的亚硫酸盐", 《食品工业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107462575A (en) * | 2017-07-25 | 2017-12-12 | 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） | A kind of sulfur dioxide quick detection kit and detection method |
| CN111257502A (en) * | 2020-03-05 | 2020-06-09 | 江苏权正检验检测有限公司 | Method for measuring sulfur dioxide in dried mushrooms |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Li et al. | Simple methods for rapid determination of sulfite in food products | |
| CN105548064B (en) | The method for producing multiple nutritional components and titer of antibodies variation during antibiotic using near infrared ray microbial fermentation | |
| CN1737544A (en) | Method for rapid detecting sodium sulfoxylate formaldehyde in foodstuff | |
| CN104483280A (en) | Method for rapidly detecting ammonia nitrogen removal rate | |
| CN102590193B (en) | Method for measuring sulfur dioxide content in food | |
| CN102590163A (en) | Method for detecting bisulfite ion | |
| CN102183518A (en) | Method for quickly measuring sulfate radical content in magnesium method desulfurization process | |
| Hosseini et al. | A novel pH optical sensor using methyl orange based on triacetylcellulose membranes as support | |
| Damirchi et al. | A comparison between digital camera and spectrophotometer for sensitive and selective kinetic determination of brilliant green in wastewaters | |
| Chao et al. | A new fluorescent enhanced probe based on (E)-9-(2-nitrovinyl)-anthracene for the detection of bisulfite anions and its practical application | |
| CN105277535B (en) | Ammonia nitrogen field fast detection method in a kind of water for eliminating reagent blank influence | |
| CN100422718C (en) | Method for determining sodium chloride content in phosphate using spectrophotometry | |
| CN104360012A (en) | Kit for simultaneously and rapidly measuring content of glucose, fructose and total sugar and application of kit | |
| CN105241876A (en) | Detection method of sulfur dioxide in wine | |
| KR101534562B1 (en) | chlorine detecting reagent and the detecting kit and the chlorine detecting method | |
| Sochorova et al. | Electrochemical and others techniques for the determination of malic acid and tartaric acid in must and wine | |
| CN105300970B (en) | Total residual chlorine field fast detection method in a kind of water for eliminating reagent blank influence | |
| CN104132920A (en) | Method for measuring Ag<+> or F<-> through fluorescence quenching | |
| CN102435560B (en) | Method for testing nitrification inhibition rate of stabilized fertilizer | |
| CN102830114B (en) | Detection method for content of tannin in plant extract liquid | |
| CN105181686A (en) | Method for detecting fluoride ions in environment | |
| Mirza Alizadeh et al. | Polarographic determination of sodium hydrosulfite residue (Dithionite) in sugar and loaf sugar | |
| CN105319209B (en) | Iron ion field fast detection method in a kind of water for eliminating reagent blank influence | |
| CN105277542B (en) | A kind of water nitrite field fast detection method for eliminating reagent blank influence | |
| Capitán-Vallvey et al. | Development of a one-shot optical citrate sensor based on a guanidinium synthetic receptor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160113 |
|
| RJ01 | Rejection of invention patent application after publication |