CN102313735A - Kit for rapidly detecting pH value of water for fishery culture and detection method thereof - Google Patents
Kit for rapidly detecting pH value of water for fishery culture and detection method thereof Download PDFInfo
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- CN102313735A CN102313735A CN201110185289A CN201110185289A CN102313735A CN 102313735 A CN102313735 A CN 102313735A CN 201110185289 A CN201110185289 A CN 201110185289A CN 201110185289 A CN201110185289 A CN 201110185289A CN 102313735 A CN102313735 A CN 102313735A
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Abstract
The invention relates to a kit for rapidly detecting the pH value of water for fishery culture and a detection method thereof. One 10 ml cuvette with stopper, one colorimetric card, one sucker, one 20ml dropping bottle, and 20ml of an acid-base indicator are arranged in the kit. A process for preparing 200ml of the acid-base indicator comprises the following steps: 1, preparing 60% ethanol: weighing 120ml of waterless ethanol, adding purified water to the volume of 200ml; 2, weighing reagents: weighing 0.15g of methyl red; 0.15g of thymol blue, 0.15g of phenolphthalein, and 0.05g of bromothymol blue, respectively dissolving above reagents with 60% ethanol, uniformly mixing, adding water to the volume of 200ml, packaging according to a standard of 20ml/ bottle. The detection method of the kit comprises the following steps: 1, flushing the cuvette 2-3 times with a water sample to be detected; 2, taking 5ml of the water sample, adding three drops of the acid-base indicator, and well shaking; and 3, covering the cuvette, slightly shaking, placing for 2-3min, placing the cuvette on the blank of the colorimetric card, and comparing with colors of the colorimetric card to obtain the pH value of the water sample to be detected. The kit can be used for on-site rapid detection of various water qualities with the pH value of 5.5-9.4.
Description
Technical field
The present invention relates to a kind of chemical constitution detection kit and detection method thereof, particularly a kind of fishery cultivating water pH value quick detection kit and detection method thereof.
Background technology
Water is the environment of depending on for existence of fish, and water not only can directly influence fish existence, also can have influence on composition, quantity and the distribution of food organisms.Good water environment is the material base that guarantees the healthy fish growth, and the water of therefore breeding fish must all be fit to the requirement that fish growth is grown at aspects such as physics, chemistry, biologies.Particularly potential of hydrogen is the pH value important indicator of fishpond water quality especially, and it has direct or indirect influence to growth, growth and the breeding etc. of fish.
When breeding fish quality of water, analysis all to measure the pH value usually; PH one has influence on the breeding production of fish to a key factor that is considered to fish-culture water quality from many aspects.The pH value that fish can live safely roughly is 6～9, and optimum scope: cyprinid fish is an alkalescent, and promptly the pH value is 7～8.5; Salmon fishes is neutral, and promptly the pH value is about in the of 7.The pH value exceeds height and is limited to 9.5～10, and lower bound is 4～5 scope, will directly cause fish kills.Breed fish to facts have proved that fish is lower than in 5.5 the water body at acid ph value responsive especially to the infectiousness fish disease, even in the water not anoxic also can occur having difficulty in breathing, to the digestibility of feed with low, poky situation.
Therefore; Based on current fishery cultivating present situation; Needing badly provides a kind of convenient, fast and detection method that susceptibility is high; Promptly developing a kind of fishery cultivating water pH value quick detection kit and detection method thereof, benefit the fishery cultivating industry, will be that this technical field scientific research personnel is badly in need of one of new problem of furtheing investigate exploitation.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, provide a kind of reasonable in design, use simple and direct, the significant fishery cultivating water of effect pH value quick detection kit and detection method thereof.
Following for realizing the embodiment that above-mentioned purpose the present invention adopted:
A kind of fishery cultivating water pH value quick detection kit is characterized in that being provided with in this kit:
10ml tool plug cuvette: 1;
Colorimetric card: 1;
Suction pipe: 1;
20ml drop bottle: 1;
Acid base indicator: 20ml;
The preparation of said acid base indicator is following: (200ml)
(1) preparation 60% ethanol: take by weighing absolute ethyl alcohol 120ml, add purified water and be settled to 200ml;
(2) weighing reagent: methyl red 0.15g; Thymol blue 0.15g; Phenolphthalein 0.15g; Bromothymol blue 0.05g, mixes respectively with after the mentioned reagent dissolving with 60% ethanol, is settled to 200ml, packing 20ml/ bottle.
The concrete operations step of using this kit test method is following:
(1) washes cuvette 2～3 times with water sample to be checked;
(2) water sampling 5ml; Adding 3 acid base indicators with suction pipe shakes up;
(3) build cuvette and also shake up gently, placed 2～3 minutes; Cuvette is affixed on the blank space of colorimetric card, with the colorimetric card color relatively get final product the pH value of the water sample of surveying.
The detection principle of kit of the present invention:, utilize different acid base indicators to show the pH value through colourimetry.Acid base indicator generally is weak organic acid or weak organic base; In different Acidity of Aikalinity solution; Their degree of ionization is different, and the ability partial ionization becomes ion and the hydrogen ion or the hydroxide ion of indicator in solution, and because structural variation; Their molecule and ion have various colors, thereby in the different solution of pH, present various colors.
The invention has the beneficial effects as follows: the present invention utilizes different acid base indicators to show the pH value, has characteristics such as accurate, quick, convenient, inexpensive, without any need for equipment, adopts several methods to measure; Be applicable to the field quick detection and the on-the-spot water analysis of multiple water quality such as fishery cultivating water, farmland irrigation water, process water, applied range; Method of application is very simple, measures reliable results; Volume is little, and is in light weight, easy to carry; Can accomplish the analytical work of a water sample in 3～5 minutes; The check and analysis expense is low; The tester need not training, only needs the by specification procedure to get final product.
Main agents in the fishery cultivating water pH value quick detection kit provided by the present invention provides with the working fluid form, and is easy to use, and reagent is easy to preserve.Acid base indicator shows various colors under different pH values, just can confirm the pH value of water quality through colourimetry.This kit can supply 100 detections to use, and effectively reduces and detects cost.Therefore, the present invention efficiently solves the demand of fast detecting water pH value in the present fishery cultivating, has solved the use pH meter and has detected and to waste time and energy and be not suitable for a difficult problem such as on-site measurement.This kit has broad application prospects in water quality detection, and has huge economic benefit and social benefit.
Embodiment
Below in conjunction with preferred embodiment, to details are as follows according to embodiment provided by the invention:
Embodiment 1
A kind of fishery cultivating water pH value quick detection kit is characterized in that being provided with in this kit:
10ml tool plug cuvette: 1;
Colorimetric card: 1; (adopting existing colorimetric card)
Suction pipe: 1;
20ml drop bottle: 1;
Acid base indicator: 20ml;
The preparation of said acid base indicator is following: (200ml)
(1) preparation 60% ethanol: take by weighing absolute ethyl alcohol 120ml, add purified water and be settled to 200ml;
(2) weighing reagent: methyl red 0.15g; Thymol blue 0.15g; Phenolphthalein 0.15g; Bromothymol blue 0.05g, mixes respectively with after the mentioned reagent dissolving with 60% ethanol, is settled to 200ml, packing 20ml/ bottle.
The concrete operations step of using this kit test method is following:
(1) washes cuvette 2～3 times with water sample to be checked;
(2) water sampling 5ml; Adding 3 acid base indicators (test fluid) with suction pipe shakes up;
(3) build cuvette and also shake up gently, placed 2～3 minutes; Cuvette is affixed on the blank space of colorimetric card, with the colorimetric card color relatively get final product the pH value of the water sample of surveying.
This kit is in 5.5～9.4 in pH value scope; Not only can be applicable to the detection of fishery cultivating water; Also can be used for the field quick detection of multiple water quality such as Drinking Water, field irrigation, environmental protection, waste water detection, aquarium fish water quality detection, use very extensive.
The acid base indicator color change interval
Title variable color pH scope change color
Methyl red: 4.8-6.0 is red-Huang;
Phenolphthalein: 8.2-10.0, colourless-light red;
Bromothymol blue: 6.0-7.6, Huang-indigo plant;
Thymol blue: 1.2-2.8 is red-Huang.
Application test analysis example 1:
The test of fishery cultivating water pH value quick detection kit precision, concrete operations are:
The fishery cultivating water pH value quick detection kit for preparing is carried out repeatability, middle precision, reappearance test respectively to test sample.Replica test: in specialized range, design 10 different pH solution (pH meter mensuration), each concentration do 3 parallel, 3 parallel all surveys 3 times of each concentration are measured the result with gained and are estimated; Middle precision test: in this laboratory, prepare the solution of above-mentioned 10 variable concentrations with distinct device by 3 different analysts, each concentration do 3 parallel, 3 parallel all surveys 3 times of each concentration are measured the result with gained and are estimated; And the mensuration result who obtains with resulting mensuration result and replica test estimates; Reappearance test: in 2 different testing laboratories, the solution of above-mentioned 10 variable concentrations is measured by different analysts.Replica test, the test of middle precision, reappearance are tested resultant test findings and evaluated, and test findings is consistent, shows that fishery cultivating water pH quick detection kit precision provided by the present invention has reached requirement.
Application test analysis example 2:
The test of fishery cultivating water pH value quick detection kit storage life:
Test of long duration: three batches of kits that prepare are put in 25 ℃ of shady and cool dry places preserve, to measuring with a collection of censorship water sample, the result measures and shows that pH practical measurement value is all within normal color range during respectively at 0,3,6,9,12 month.
Accelerated test: three batches of kits that prepare are put in 40 ℃ of constant temperature ovens placed for 2 weeks, carry out accelerated aging test, then to measuring with a collection of censorship water sample, the result shows and treats that pH practical measurement value is all within normal color range in the test sample.
Above-mentioned detailed description of this fishery cultivating water pH value quick detection kit and detection method thereof being carried out with reference to embodiment; Be illustrative rather than determinate; Can enumerate out several embodiment according to institute's limited range; Therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (2)
1. fishery cultivating water pH value quick detection kit is characterized in that being provided with in this kit:
10ml tool plug cuvette: 1;
Colorimetric card: 1;
Suction pipe: 1;
20ml drop bottle: 1;
Acid base indicator: 20ml;
The preparation of said acid base indicator is following:
(1) preparation 60% ethanol: take by weighing absolute ethyl alcohol 120ml, add purified water and be settled to 200ml;
(2) weighing reagent: methyl red 0.15g; Thymol blue 0.15g; Phenolphthalein 0.15g; Bromothymol blue 0.05g, mixes respectively with after the mentioned reagent dissolving with 60% ethanol, is settled to 200ml, packing 20ml/ bottle.
2. the detection method of fishery cultivating water pH value quick detection kit according to claim 1 is characterized in that operation steps is following:
(1) washes cuvette 2～3 times with water sample to be checked;
(2) water sampling 5ml; Adding 3 acid base indicators with suction pipe shakes up;
(3) build cuvette and also shake up gently, placed 2～3 minutes; Cuvette is affixed on the blank space of colorimetric card, with the colorimetric card color relatively get final product the pH value of the water sample of surveying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110185289A CN102313735A (en) | 2011-07-04 | 2011-07-04 | Kit for rapidly detecting pH value of water for fishery culture and detection method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110185289A CN102313735A (en) | 2011-07-04 | 2011-07-04 | Kit for rapidly detecting pH value of water for fishery culture and detection method thereof |
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| CN102313735A true CN102313735A (en) | 2012-01-11 |
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| CN201110185289A Pending CN102313735A (en) | 2011-07-04 | 2011-07-04 | Kit for rapidly detecting pH value of water for fishery culture and detection method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104237346A (en) * | 2014-10-09 | 2014-12-24 | 无锡百灵传感技术有限公司 | Electrochemical sensor for detecting pH value of purified water online |
| CN104345057A (en) * | 2013-08-05 | 2015-02-11 | 上海索诚化学试剂有限公司 | Test paper for detecting residual chlorine, pH value and total alkalinity in water |
| CN105021610A (en) * | 2014-09-29 | 2015-11-04 | 邓光 | Method for detecting pH value of water |
| CN105203541A (en) * | 2015-09-18 | 2015-12-30 | 浙江大学 | Pancreatic leakage detection material based on acid-base indication compound chemical color changes and application thereof |
| CN106996931A (en) * | 2016-01-26 | 2017-08-01 | 福建大北农水产科技有限公司 | A kind of pH quick detection kits of breeding water body |
| CN108072651A (en) * | 2016-11-28 | 2018-05-25 | 上海泓宝绿色水产股份有限公司 | A kind of total alkalinity detecting system and its application method for being used to detect that total alkalinity is 0-300mg/L |
| WO2020148640A3 (en) * | 2019-01-15 | 2020-09-03 | Precision Planting Llc | Soil analysis compositions and methods |
-
2011
- 2011-07-04 CN CN201110185289A patent/CN102313735A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104345057A (en) * | 2013-08-05 | 2015-02-11 | 上海索诚化学试剂有限公司 | Test paper for detecting residual chlorine, pH value and total alkalinity in water |
| CN105021610A (en) * | 2014-09-29 | 2015-11-04 | 邓光 | Method for detecting pH value of water |
| CN104237346A (en) * | 2014-10-09 | 2014-12-24 | 无锡百灵传感技术有限公司 | Electrochemical sensor for detecting pH value of purified water online |
| CN104237346B (en) * | 2014-10-09 | 2016-08-24 | 无锡百灵传感技术有限公司 | The electrochemical sensor of on-line checking purified water pH value |
| CN105203541A (en) * | 2015-09-18 | 2015-12-30 | 浙江大学 | Pancreatic leakage detection material based on acid-base indication compound chemical color changes and application thereof |
| CN106996931A (en) * | 2016-01-26 | 2017-08-01 | 福建大北农水产科技有限公司 | A kind of pH quick detection kits of breeding water body |
| CN108072651A (en) * | 2016-11-28 | 2018-05-25 | 上海泓宝绿色水产股份有限公司 | A kind of total alkalinity detecting system and its application method for being used to detect that total alkalinity is 0-300mg/L |
| WO2020148640A3 (en) * | 2019-01-15 | 2020-09-03 | Precision Planting Llc | Soil analysis compositions and methods |
| CN113227786A (en) * | 2019-01-15 | 2021-08-06 | 精密种植有限责任公司 | Soil analysis compositions and methods |
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Application publication date: 20120111 |