CN102393392A - Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof - Google Patents
Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof Download PDFInfo
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- CN102393392A CN102393392A CN2011103282896A CN201110328289A CN102393392A CN 102393392 A CN102393392 A CN 102393392A CN 2011103282896 A CN2011103282896 A CN 2011103282896A CN 201110328289 A CN201110328289 A CN 201110328289A CN 102393392 A CN102393392 A CN 102393392A
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Abstract
The invention relates to a reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture, which is internally provided with a 10ml of colorimetric cup with a plug, a colorimetric card, a suction tube, two 20ml of dropping bottles, 20ml of A reagent (sodium potassium tartrate tetrahydrate) and 20ml of B reagent (Nash reagent). A reagent detection method has the following particular steps: firstly, cleaning the colorimetric cup for two to three times by water to be detected; secondly, taking 5ml of water samples, using the suction tube to add five drops of A reagent (sodium potassium tartrate tetrahydrate), then adding five drops of B reagent (Nash reagent), and stirring uniformly; and thirdly, covering the colorimetric cup and shaking the colorimetric cup uniformly, and standing for 1-3minutes; and adhering the colorimetric cup at the blank of the colorimetric card, and comparing the color of the colorimetric cup with that of the colorimetric card, thus obtaining the ammonium and nitrogen content (mg/L) of the water sample to be detected. The reagent kit has the advantages of accuracy, rapidness, convenience, low cost and the like, does not any devices, adopts drop counting method to detect, is used for the rapid site detection of a plurality of kinds of water such as aquaculture water, domestic sewage, industrial waste water and the like; and the using method is extremely simple.
Description
Technical field
The present invention relates to a kind of chemical constitution detection kit and detection method thereof, particularly a kind of aquaculture water ammonia nitrogen quick detection kit and detection method thereof.
Background technology
In the aquaculture process, we often run into the too high problem of ammonia nitrogen in the pond, and this problem is more serious in the high density fishing breeding pond, has caused certain harm to breed.Ammonia nitrogen in the pond is mainly derived from three kinds of approach: the excreta of (1) aquatic animal, the fertilizer that applies, residual bait, animals and plants corpse contain amounts of protein, are decomposed the amino acid that the back forms by the microbial bacteria in the pond, further resolve into ammonia nitrogen again; (2) when oxygen is not enough, water body generation anti-nitration reaction, nitrite, nitrate decompose under the effect of denitrifying bacteria and produce ammonia nitrogen; (3) fish can get rid of the ammonia nitrogen in the body through the gill and antennal gland through the gill and urine, shell-fish in water, to avoid ammonia poisoning in the body.
Ammonia nitrogen is present in the water with two kinds of forms, and a kind of is ammonia (NH
3), be non-ionic ammonia again, fat-soluble, poisonous to hydrobiont.Another kind is ammonia (NH
4 +), be ion ammonia again, nontoxic to hydrobiont.Ammonia nitrogen is to the harm of aquatic animal: the reduction of ingesting, decreased growth; Tissue damage reduces the conveying of oxygen between tissue; Fish and shrimp all need carry out ion-exchange (sodium, calcium etc.) with water body, the too high permeability that can increase the gill of ammonia nitrogen, the ion exchanging function of the infringement gill; Make hydrobiont be in stress situation for a long time, increase the neurological susceptibility of animal, reduce the speed of growth disease; Reduce fecundity, reduce fish brood amount, reduce the viability of ovum, postpone egg reproduction.The harm that acute ammonia nitrogen is poisoned: hydrobiont shows as excited, balance, tic, severe patient even death in water.
Therefore; Based on pressing for of existing situation; Needing badly provides a kind of convenient, fast and detection method that susceptibility is high; Promptly developing a kind of aquaculture water ammonia nitrogen quick detection kit and detection method thereof, benefit culture fishery, will be that this technical field scientific research personnel is badly in need of one of new problem of furtheing investigate exploitation.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, the significant aquaculture water of a kind of effect ammonia nitrogen quick detection kit and detection method thereof are provided.
Following for realizing the embodiment that above-mentioned purpose the present invention adopted:
A kind of aquaculture water ammonia nitrogen quick detection kit is characterized in that being provided with in this kit:
10ml tool plug cuvette: 1;
Colorimetric card: 1;
Suction pipe: 1;
20ml drop bottle: 2;
A reagent is sodium potassium tartrate tetrahydrate: 20ml;
B reagent is for receiving assorted reagent: 20ml;
The preparation of sodium potassium tartrate tetrahydrate (100ml): take by weighing the 50g sodium potassium tartrate tetrahydrate and be dissolved in 100ml and do not have in the ammoniacal liquor, add the NaOH 5ml of 250g/L, heated and boiled, cooling back are settled to 100ml with deionized water, packing, and the 20ml/ bottle keeps in Dark Place;
Receive the preparation (400ml) of assorted reagent: take by weighing the 20g potassium iodide and be dissolved in 25ml and do not have in the ammoniacal liquor, the limit is stirred and is just added 8.2g HgCl
2Be stirred well to resolution of precipitate; Other gets 60g NaOH and is dissolved in the 250ml deionized water, and mix cooling back, and is diluted to 400ml with deionized water, treat the solution clarification after, get the supernatant liquid body in brown bottle, packing, the 20ml/ bottle keeps in Dark Place.
The concrete operations step of this kit test method is following:
(1) washes cuvette 2～3 times with water to be checked;
(2) water sampling 5ml; Adding 5 A reagent with suction pipe is sodium potassium tartrate tetrahydrate, adds 5 B reagent again and promptly receives assorted reagent and shake up;
(3) build cuvette and also shake up gently, placed 1～3 minute; Cuvette is affixed on the blank space of colorimetric card, with the colorimetric card color relatively get final product the ammonia-nitrogen content (mg/L) of the water sample of surveying.
The detection principle of kit of the present invention: the ammonium nitrogen and the nessler reagent reaction that exist with forms such as free ammonia or ammonium ions generate the yellowish-brown complex compound, and the colourity of this complex compound is directly proportional with the content of ammonium nitrogen, available visual colorimetric determination and spectrophotometry.When visual colorimetry was measured, concentration limit was 0.02mg/L, and upper limit concentration is 2 mg/L; During spectrophotometry, concentration limit is 0.05 mg/L, and upper limit concentration is 2 mg/L.This ammonia nitrogen quick detection kit sensing range is 0.1～1.8 mg/L.
The invention has the beneficial effects as follows: kit of the present invention is to utilize to receive assorted reagent and show ammonia-nitrogen content in the water sample to be measured, has characteristics such as accurate, quick, convenient, inexpensive, without any need for equipment, adopts several methods to measure; Can be used for aquaculture water, Drinking Water and source water thereof; Surface water, seawater; The field quick detection of multiple water quality such as sanitary sewage and industrial waste water; Method of application is very simple, measures reliable results; Volume is little, and is in light weight, easy to carry; Can accomplish the analytical work of a water sample in 3～5 minutes; The check and analysis expense is low; The tester need not training, only needs the by specification procedure to get final product.
Main agents in ammonia nitrogen quick detection kit provided by the present invention and the detection method thereof provides with the working fluid form, and is easy to use, and reagent is easy to preserve.The alkaline solution of mercuric iodixde and potassium iodide and ammonia reflection generate light red brown colloidization compound, and its colourity is directly proportional with ammonia-nitrogen content, adopts visual colorimetry, and concentration limit is 0.02mg/L.This kit can supply 80 detections to use, and effectively reduces and detects cost.Therefore, the present invention efficiently solves the demand of fast detecting water quality ammonia nitrogen in the present aquaculture, and having solved use ammonia nitrogen detector wastes time and energy and be not suitable for difficult problems such as on-site measurement.This kit has broad application prospects in the water quality detection field, and has huge economic benefit and social benefit.
Embodiment
Below in conjunction with preferred embodiment, to details are as follows according to embodiment provided by the invention:
Embodiment 1
A kind of aquaculture water ammonia nitrogen quick detection kit is characterized in that being provided with in this kit:
10ml tool plug cuvette: 1;
Colorimetric card: 1; (adopting existing colorimetric card)
Suction pipe: 1;
20ml drop bottle: 2;
A reagent is sodium potassium tartrate tetrahydrate: 20ml;
B reagent is for receiving assorted reagent: 20ml;
The preparation of sodium potassium tartrate tetrahydrate (100ml): take by weighing the 50g sodium potassium tartrate tetrahydrate and be dissolved in 100ml and do not have in the ammoniacal liquor, add the NaOH 5ml of 250g/L, heated and boiled, cooling back are settled to 100ml with deionized water, packing, and the 20ml/ bottle keeps in Dark Place;
Receive the preparation (400ml) of assorted reagent: take by weighing 20g potassium iodide (KI) and be dissolved in 25ml and do not have in the ammoniacal liquor, the limit is stirred and is just added 8.2g HgCl
2Be stirred well to resolution of precipitate; Other gets 60g NaOH and is dissolved in the 250ml deionized water, and mix cooling back, and is diluted to 400ml with deionized water, treat the solution clarification after, get the supernatant liquid body in brown bottle, packing, the 20ml/ bottle keeps in Dark Place.
The concrete operations step of this kit test method is following:
(1) washes cuvette 2～3 times with water to be checked;
(2) water sampling 5ml; Add 5 A reagent (sodium potassium tartrate tetrahydrate) with suction pipe, add 5 B reagent (the assorted reagent of receiving shakes up) again;
(3) build cuvette and also shake up gently, placed 1～3 minute; Cuvette is affixed on the blank space of colorimetric card, with the colorimetric card color relatively get final product the ammonia-nitrogen content (mg/L) of the water sample of surveying.
This ammonia nitrogen quick detection kit sensing range is 0.1～1.8 mg/L, is used for Drinking Water and source water thereof; Surface water, aquaculture water, seawater; The field quick detection of multiple water quality such as sanitary sewage and industrial waste water.
Application test analysis example 1:
The test of ammonia nitrogen quick detection kit precision, concrete operations are:
The ammonia nitrogen quick detection kit for preparing is carried out repeatability, middle precision, reappearance test respectively to test sample.Replica test: in specialized range, the solution (spectrophotometric determination) of 8 different ammonia-nitrogen contents of design, each concentration do 3 parallel, 3 parallel all surveys 3 times of each concentration are measured the result with gained and are estimated; Middle precision test: in this laboratory, prepare the solution of above-mentioned 8 variable concentrations with distinct device by 3 different analysts, each concentration do 3 parallel, 3 parallel all surveys 3 times of each concentration are measured the result with gained and are estimated; And the mensuration result who obtains with resulting mensuration result and replica test estimates; Reappearance test: in 2 different testing laboratories, the solution of above-mentioned 8 variable concentrations is measured by different analysts.Replica test, the test of middle precision, reappearance are tested resultant test findings and evaluated, and test findings is consistent, shows that ammonia nitrogen quick detection kit precision provided by the present invention has reached requirement.
Application test analysis example 2:
The test of ammonia nitrogen quick detection kit storage life:
Test of long duration: three batches of kits that prepare are put in 4 ℃, keep in Dark Place, to measuring with a collection of censorship water sample, the result shows that ammonia-nitrogen content practical measurement value conforms to the spectrophotometric determination value during respectively at 0,3,6,9,12 month.
Above-mentioned detailed description of this aquaculture water ammonia nitrogen quick detection kit and detection method thereof being carried out with reference to embodiment; Be illustrative rather than determinate; Can enumerate out several embodiment according to institute's limited range; Therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (2)
1. aquaculture water ammonia nitrogen quick detection kit is characterized in that being provided with in this kit:
10ml tool plug cuvette: 1;
Colorimetric card: 1;
Suction pipe: 1;
20ml drop bottle: 2;
A reagent is sodium potassium tartrate tetrahydrate: 20ml;
B reagent is for receiving assorted reagent: 20ml;
The preparation 100ml of sodium potassium tartrate tetrahydrate: take by weighing the 50g sodium potassium tartrate tetrahydrate and be dissolved in 100ml and do not have in the ammoniacal liquor, add the NaOH 5ml of 250g/L, heated and boiled, cooling back are settled to 100ml with deionized water, packing, and the 20ml/ bottle keeps in Dark Place;
Receive the preparation 400ml of assorted reagent: take by weighing the 20g potassium iodide and be dissolved in 25ml and do not have in the ammoniacal liquor, the limit is stirred and is just added 8.2g HgCl
2Be stirred well to resolution of precipitate; Other gets 60g NaOH and is dissolved in the 250ml deionized water, and mix cooling back, and is diluted to 400ml with deionized water, treat the solution clarification after, get the supernatant liquid body in brown bottle, packing, the 20ml/ bottle keeps in Dark Place.
2. the detection method of aquaculture water ammonia nitrogen quick detection kit according to claim 1 is characterized in that operation steps is following:
(1) washes cuvette 2～3 times with water to be checked;
(2) water sampling 5ml; Adding 5 A reagent with suction pipe is sodium potassium tartrate tetrahydrate, adds 5 B reagent again and promptly receives assorted reagent and shake up;
(3) build cuvette and also shake up gently, placed 1～3 minute; Cuvette is affixed on the blank space of colorimetric card, with the colorimetric card color relatively get final product the ammonia-nitrogen content mg/L of the water sample of surveying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103282896A CN102393392A (en) | 2011-10-26 | 2011-10-26 | Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof |
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| CN2011103282896A CN102393392A (en) | 2011-10-26 | 2011-10-26 | Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof |
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| CN102393392A true CN102393392A (en) | 2012-03-28 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070112A (en) * | 2013-01-08 | 2013-05-01 | 汕头大学 | Manufacturing method of scallop color fan for measuring total carotenoid content in adductor muscles of scallops and application of scallop color fan |
| CN103091148A (en) * | 2013-01-08 | 2013-05-08 | 北京工业大学 | Pretreatment method for measuring ammonia nitrogen in living sewage |
| CN104374771A (en) * | 2014-11-19 | 2015-02-25 | 阜阳师范学院 | Kit for rapidly detecting ammonia nitrogen content in water and detection method of kit |
| CN105021599A (en) * | 2014-09-29 | 2015-11-04 | 邓光 | Method for detecting ammonia nitrogen in water |
| CN107132216A (en) * | 2016-02-29 | 2017-09-05 | 福建大北农水产科技有限公司 | A kind of breeding water body ammonia nitrogen quick detection kit |
| CN107703128A (en) * | 2017-09-08 | 2018-02-16 | 广州市赛特检测有限公司 | The quick determination method of ammonia in a kind of air |
| CN111328757A (en) * | 2020-02-18 | 2020-06-26 | 江苏大学 | Greenhouse ecological cycle three-dimensional planting and breeding system and method |
| CN113916815A (en) * | 2021-10-13 | 2022-01-11 | 海南大学 | Method for measuring content of ammonia nitrogen in seawater by using Nashin's reagent spectrophotometry |
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| JP2000275234A (en) * | 1999-03-26 | 2000-10-06 | Horiba Ltd | Analyzing method for ammoniacal nitrogen and total nitrogen |
| JP2003014722A (en) * | 2001-07-02 | 2003-01-15 | Meidensha Corp | Ammonia measurement waste liquid treatment method and ammonia measurement apparatus |
| CN201955295U (en) * | 2010-12-15 | 2011-08-31 | 北京桑普生物化学技术有限公司 | Multiple-index water quality test box |
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2011
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| JP2000275234A (en) * | 1999-03-26 | 2000-10-06 | Horiba Ltd | Analyzing method for ammoniacal nitrogen and total nitrogen |
| JP2003014722A (en) * | 2001-07-02 | 2003-01-15 | Meidensha Corp | Ammonia measurement waste liquid treatment method and ammonia measurement apparatus |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070112A (en) * | 2013-01-08 | 2013-05-01 | 汕头大学 | Manufacturing method of scallop color fan for measuring total carotenoid content in adductor muscles of scallops and application of scallop color fan |
| CN103091148A (en) * | 2013-01-08 | 2013-05-08 | 北京工业大学 | Pretreatment method for measuring ammonia nitrogen in living sewage |
| CN103070112B (en) * | 2013-01-08 | 2015-01-07 | 汕头大学 | Manufacturing method of scallop color fan for measuring total carotenoid content in adductor muscles of scallops and application of scallop color fan |
| CN105021599A (en) * | 2014-09-29 | 2015-11-04 | 邓光 | Method for detecting ammonia nitrogen in water |
| CN104374771A (en) * | 2014-11-19 | 2015-02-25 | 阜阳师范学院 | Kit for rapidly detecting ammonia nitrogen content in water and detection method of kit |
| CN107132216A (en) * | 2016-02-29 | 2017-09-05 | 福建大北农水产科技有限公司 | A kind of breeding water body ammonia nitrogen quick detection kit |
| CN107703128A (en) * | 2017-09-08 | 2018-02-16 | 广州市赛特检测有限公司 | The quick determination method of ammonia in a kind of air |
| CN111328757A (en) * | 2020-02-18 | 2020-06-26 | 江苏大学 | Greenhouse ecological cycle three-dimensional planting and breeding system and method |
| CN113916815A (en) * | 2021-10-13 | 2022-01-11 | 海南大学 | Method for measuring content of ammonia nitrogen in seawater by using Nashin's reagent spectrophotometry |
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Application publication date: 20120328 |