CN104374771A - Kit for rapidly detecting ammonia nitrogen content in water and detection method of kit - Google Patents
Kit for rapidly detecting ammonia nitrogen content in water and detection method of kit Download PDFInfo
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- CN104374771A CN104374771A CN201410662577.9A CN201410662577A CN104374771A CN 104374771 A CN104374771 A CN 104374771A CN 201410662577 A CN201410662577 A CN 201410662577A CN 104374771 A CN104374771 A CN 104374771A
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Abstract
The invention discloses a kit for rapidly detecting the ammonia nitrogen content in water and a detection method of the kit. The kit comprises a developing cup, a liquid sampler, a standard colorimetric plate, a detection reagent A liquid, a detection reagent B liquid and non-ammonia water, wherein the developing cup is a transparent cuboid; the cuboid is internally provided with four long grooves which are connected into a row, and the cross sections of the long grooves are square; the four long grooves are cuboids with the same volume. The kit is provided with a set of complete test agents and vessels, and can be used for detecting the concentration of the ammonia nitrogen in surface water, underground water, domestic sewage and industrial wastewater.
Description
Technical field
The present invention relates to a kind of kit and detection method thereof of quick detection water body ammonia-nitrogen content, belong to chemical field.
Background technology
Ammonia nitrogen detects recycling and in objectionable impurities qualification, being seized of very important status at domestic water or waste water.In current mensuration water body, the common method of ammonia nitrogen has colourimetry (comprising Berthelot spectrophotometry, phenol-hypochlorite colourimetry, salicylic acid-hypochlorite colourimetry) and distillation titration method, the chromatography of ions, Using Flow Injection Analysis, Ammonia Gas Sensor Electrode Method etc.Wherein paying close attention to the most with Berthelot spectrophotometry, is at present as conventional classical way, and the standard method that country formulates, and one of analytical approach that Ye Shi China " water environment quality standard " specifies, belongs to the first-selected means measuring ammonia nitrogen concentration.
Nessler's reagent photometer is by obtaining purer ammonia, then according to the ammonia in water and nessler reagent (K after flocculent precipitation (master is applicable to clean water) or the way of distillation (being suitable for serious pollution water sample or industrial waste water etc.) pre-service water sample to be checked
2hgI
4) the light red brown colloidization compound of reaction generation in strongly basic medium, its colourity is directly proportional to ammonia-nitrogen content, usually can survey its absorbance within the scope of wavelength 410 ~ 425nm, is calculated the content of ammonia nitrogen in water by absorbance.This law is easy and simple to handle, sensitivity is higher (by national standard 50ml water determination then lowest detection mass concentration be 0.1mg/L), wide accommodation, be applicable to the mensuration of surface water, underground water, industrial waste water and Ammonia Nitrogen in Municipal Wastewater content, but this method need use toxic reagent tetraiodo mercury potassium and highly basic, to operating personnel and environmental impact larger.
Its principle of salicylate colourimetry is under sodium nitroprusside (being commonly called as sodium nitroprussiate) catalysis, ammonia and hypochlorite react and generate monochloro amine, monochloro amine and salicylate react and generate blue compound dichloro fulminuric acid sodium, carry out photometry colorimetric estimation quantitative in 679nm wavelength.Measure before pre-service and Na Shi method similar, the blue compound stability that this method generates is better, Absorbable organic halogens 24h, sensitivity and precision are also higher, but because of its reagent can not standing time long, in water, ammonia can react with chlorine and generate dichloro ammonia or trichlorine ammonia, and make measurement result on the low side, the recovery is lower.So temperature and color development time length affect larger on sample accuracy.This law lowest detection mass concentration is 0.01mg/L, and the range of linearity upper limit is 1mg/L.Be mainly used in the mensuration of ammonia nitrogen in the water samples such as potable water.All there is operation steps complexity in above-mentioned two kinds of sides, long and time and temperature the interference of analytical cycle is larger.The metallic ions such as the calcium in addition in water, magnesium and iron, sulfide, aldehyde and ketone, color and muddiness etc. are interference measurement all, all needs to do corresponding pre-service.
Distillation-titrimetry be applicable to ammonia-nitrogen content higher time mensuration.But distillation needs the regular hour, and the time of mensuration is long, and it is more to gather water sample, and reagent dosage is also just many.Other several method then needs expensive instrument.And these methods are all just can complete in laboratory conditions, be equipped with relevant detection operating personnel, on-the-spot mensuration also cannot be carried out.
Summary of the invention
The object of the invention overcomes above-mentioned defect exactly, and provide a kind of detection kit and the detection method thereof that quick and precisely can detect ammonia-nitrogen content in water body easily, to solve the harmful effect of toxic reagent, simplify the operation step, improves detection efficiency.
Object of the present invention is just to provide a kind of kit of quick detection water body ammonia-nitrogen content.
Another object of the present invention just there is provided the detection method of this test box.
Comprise in kit of the present invention: colour developing cup, liquid getting device and standard colorimetric plate, detection reagent A liquid and B liquid, without ammoniacal liquor, colour developing cup is 1 transparent rectangular parallelepiped, in this rectangular parallelepiped, be provided with 4 xsects being connected in a row is foursquare long body groove, four long body grooves are all the rectangular parallelepipeds that volume is identical, length is 1cm × 1cm × 3cm, volume is 3mL, the colour developing of sample to be tested and blank during for detecting; Liquid getting device comprises the utensil of 4 1mL capacity; 1 piece of standard colorimetric plate is provided with the colour developing card that 3 row's colors change in gradient, for contrasting the colour developing result of different ammonia nitrogen concentration.Detecting reagent A liquid and each 1 bottle of B liquid, is the reagent being used as colour developing; 1 bottle is done blank without ammoniacal liquor and uses.Measuring sample to be tested with liquid getting device during detection adds in the long body groove of colour developing cup, wherein add identical sample to be tested in 1-3 groove, add in 4th long body groove equivalent without ammoniacal liquor, then in 4 long body grooves, A liquid is added respectively, mixing, add B liquid respectively again, 10-15min is placed in mixing, and reference standard color board can judge institute's sample ammonia-nitrogen content originally.
Beneficial effect of the present invention: one is adopt shaping kit package, has detection reagent and the vessel of complete set, does not need to spend a large amount of various reagent of time deallocation system again; Two is do not need to calculate ammonia-nitrogen content in water body by doing typical curve again, and the standard colorimetric plate that kit provides directly can obtain the ammonia-nitrogen content measuring sample; Three is that the detection sample of agents useful for same and required collection is all less, the colour developing cup detected and liquid getting device easy to clean; Four be detect colour developing cup be provided with three glasss simultaneously detect and a blank as colourless contrast, ensure that accuracy and the reliability of testing result; Five is colour atlas that standard colorimetric plate contains 3 row's colors and changes in gradient, this for detect low, neutralize high three kinds of different ammonia-nitrogen content water bodys and design, so the having wide range of applications of this kit, the water body being applicable to various ammonia-nitrogen content detects.
The present invention is applicable to the concentration detecting surface water, underground water, sanitary sewage and ammonia nitrogen in industrial waste water, and sensing range is wide, and speed is fast, energy-and time-economizing, efficiency is high, and accurately, Site Detection speed is fast for detection sensitivity and degree of accuracy, easy operation, has a extensive future, and is convenient to promote.
Be described in further detail below in conjunction with accompanying drawing.
Accompanying drawing explanation
Fig. 1 is colour developing cup structure schematic diagram;
Fig. 2 is colour developing cup cross-sectional view;
Fig. 3 is standard colorimetric plate front schematic view.
Embodiment
Colour developing cup 5 shown in Fig. 1-2 is transparent rectangular parallelepipeds, being provided with four xsects being connected in a row in rectangular parallelepiped is foursquare long body groove, four long body grooves 1,2,3,4 are all the rectangular parallelepipeds that volume is identical, and length × wide × height is 1cm × 1cm × 3cm, and volume is 3mL.Measure with liquid getting device the long body groove that sample to be tested puts into colour developing cup during detection, wherein 1,2,3 long body grooves add identical sample to be tested, 4th long body groove 4 add equivalent without ammoniacal liquor, then in 4 long body grooves, A liquid is added respectively, mixing, add B liquid respectively again, 10-15min is placed in mixing, and reference standard color board can judge institute's sample ammonia-nitrogen content originally.
Standard colorimetric plate 6 shown in Fig. 3 is provided with grid colour developing card and the grade numeral 7 of basic, normal, high three kinds of different concentration, is respectively equipped with colors different from shallow to deep and distinguishes in basic, normal, high grid colour developing card.
Embodiment one
The detection of low concentration: the sample to be tested that during detection, the liquid getting device of 1mL measures 1mL puts into the long body groove of colour developing cup, wherein 1,2,3 long body grooves add the identical sample to be tested of 1mL respectively, 4th long body groove 4 add 1mL without ammoniacal liquor, then in 4 long body grooves, add the A liquid of 15 μ L respectively, mixing, add the B liquid of 20 μ L more respectively, 10-15min is placed in mixing, and the colour developing card in reference standard color board corresponding to low concentration can judge institute's sample ammonia-nitrogen content originally.
Embodiment two
The detection of middle concentration: the sample to be tested that during detection, the liquid getting device of 1mL measures 1mL puts into the long body groove of colour developing cup, wherein 1,2, No. 3 long body groove adds the identical sample to be tested of 1mL respectively, 4th long body groove 4 add 1mL without ammoniacal liquor, then in 4 long body grooves, add the A liquid of 150 μ L respectively, mixing, add the B liquid of 200 μ L more respectively, 10-15min is placed in mixing, and the colour developing card in reference standard color board corresponding to concentration can judge institute's sample ammonia-nitrogen content originally.
Embodiment three
The detection of high concentration: the sample to be tested that during detection, the liquid getting device of 1mL measures 0.5mL puts into the long body groove of colour developing cup, wherein 1,2,3 long body grooves add the identical sample to be tested of 0.5mL respectively, 4th long body groove 4 add 0.5mL without ammoniacal liquor, then in 4 long body grooves, add the A liquid of 750 μ L respectively, mixing, add the B liquid of 1000 μ L more respectively, 10-15min is placed in mixing, and the colour developing card corresponding to reference standard color board middle and high concentration can judge institute's sample ammonia-nitrogen content originally.
Claims (6)
1. one kind is detected the kit of water body ammonia-nitrogen content fast, comprise colour developing cup, liquid getting device and standard colorimetric plate, detection reagent A liquid and B liquid, without ammoniacal liquor, it is characterized in that: described colour developing cup is 1 transparent rectangular parallelepiped, being provided with 4 xsects being connected in a row in rectangular parallelepiped is foursquare long body groove, four long body grooves are all the rectangular parallelepipeds that volume is identical, length is 1cm × 1cm × 3cm, and volume is 3mL, and liquid getting device comprises the utensil of 4 1mL capacity; 1 piece of standard colorimetric plate is provided with the colour developing card that 3 row's colors change in gradient, and detect reagent A liquid and each 1 bottle of B liquid, 1 bottle without ammoniacal liquor.
2. the kit of quick detection water body ammonia-nitrogen content according to claim 1, it is characterized in that: described standard colorimetric plate (6) is provided with grid colour developing card and the grade numeral (7) of basic, normal, high three kinds of variable concentrations, be respectively equipped with colors different from shallow to deep in basic, normal, high grid colour developing card and distinguish.
3. one kind is detected the detection method of water body ammonia-nitrogen content fast, it is characterized in that: during detection, measure with liquid getting device the long body groove that sample to be tested puts into colour developing cup, wherein (1), (2), (3) individual long body groove add identical sample to be tested, 4th long body groove (4) add equivalent without ammoniacal liquor, then in 4 long body grooves, add A liquid respectively, mixing, then add B liquid respectively, 10-15min is placed in mixing, and reference standard color board can judge institute's sample ammonia-nitrogen content originally.
4. the detection method of quick detection water body ammonia-nitrogen content according to claim 3, it is characterized in that: the detection of low concentration: the sample to be tested that during detection, the liquid getting device of 1mL measures 1mL puts into the long body groove of colour developing cup, wherein (1), (2), (3) long body groove adds the identical sample to be tested of 1mL respectively, 4th long body groove 4 add 1mL without ammoniacal liquor, then in 4 long body grooves, add the A liquid of 15 μ L respectively, mixing, add the B liquid of 20 μ L more respectively, 10-15min is placed in mixing, colour developing card in reference standard color board corresponding to low concentration can judge institute's sample ammonia-nitrogen content originally.
5. the detection method of quick detection water body ammonia-nitrogen content according to claim 3, it is characterized in that: the detection of described middle concentration: the sample to be tested that during detection, the liquid getting device of 1mL measures 1mL puts into the long body groove of colour developing cup, wherein (1), (2), (3) a number long body groove adds the identical sample to be tested of 1mL respectively, 4th long body groove 4 add 1mL without ammoniacal liquor, then in 4 long body grooves, add the A liquid of 150 μ L respectively, mixing, add the B liquid of 200 μ L more respectively, 10-15min is placed in mixing, colour developing card corresponding to the upper concentration of reference standard color board can judge institute's sample ammonia-nitrogen content originally.
6. the detection method of quick detection water body ammonia-nitrogen content according to claim 3, it is characterized in that: the detection of described high concentration: the sample to be tested that during detection, the liquid getting device of 1mL measures 0.5mL puts into the long body groove of colour developing cup, wherein (1), (2), (3) long body groove adds the identical sample to be tested of 0.5mL respectively, 4th long body groove 4 add 0.5mL without ammoniacal liquor, then in 4 long body grooves, add the A liquid of 750 μ L respectively, mixing, add the B liquid of 1000 μ L more respectively, 10-15min is placed in mixing, colour developing card corresponding to reference standard color board middle and high concentration can judge institute's sample ammonia-nitrogen content originally.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410662577.9A CN104374771B (en) | 2014-11-19 | 2014-11-19 | A kind of kit and its detection method of quick detection water body ammonia-nitrogen content |
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| CN201410662577.9A CN104374771B (en) | 2014-11-19 | 2014-11-19 | A kind of kit and its detection method of quick detection water body ammonia-nitrogen content |
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| CN104374771A true CN104374771A (en) | 2015-02-25 |
| CN104374771B CN104374771B (en) | 2018-03-09 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113391021A (en) * | 2021-03-31 | 2021-09-14 | 赵永刚 | Kit for determining ammonia nitrogen in sample by ion chromatography post-column derivatization method and detection method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000275234A (en) * | 1999-03-26 | 2000-10-06 | Horiba Ltd | Analyzing method for ammoniacal nitrogen and total nitrogen |
| CN102033063A (en) * | 2010-10-28 | 2011-04-27 | 上海绿帝环保科技有限公司 | Preparation and use method of color comparison detection piece for ammonia nitrogen in water |
| CN102393392A (en) * | 2011-10-26 | 2012-03-28 | 天津生机集团股份有限公司 | Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof |
| CN202204820U (en) * | 2011-08-12 | 2012-04-25 | 石家庄禾柏生物技术股份有限公司 | Integrated combined kit |
| CN102654462A (en) * | 2012-01-29 | 2012-09-05 | 常州友邦净水材料有限公司 | TMB (tetramethylbenzidine) residual chlorine comparator |
| CN104034681A (en) * | 2014-06-26 | 2014-09-10 | 南通永康检测技术有限公司 | Method for detecting content of ammonia nitrogen in sewage |
| CN204203105U (en) * | 2014-11-19 | 2015-03-11 | 阜阳师范学院 | Water body ammonia-nitrogen content speed check reagent box |
-
2014
- 2014-11-19 CN CN201410662577.9A patent/CN104374771B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000275234A (en) * | 1999-03-26 | 2000-10-06 | Horiba Ltd | Analyzing method for ammoniacal nitrogen and total nitrogen |
| CN102033063A (en) * | 2010-10-28 | 2011-04-27 | 上海绿帝环保科技有限公司 | Preparation and use method of color comparison detection piece for ammonia nitrogen in water |
| CN202204820U (en) * | 2011-08-12 | 2012-04-25 | 石家庄禾柏生物技术股份有限公司 | Integrated combined kit |
| CN102393392A (en) * | 2011-10-26 | 2012-03-28 | 天津生机集团股份有限公司 | Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof |
| CN102654462A (en) * | 2012-01-29 | 2012-09-05 | 常州友邦净水材料有限公司 | TMB (tetramethylbenzidine) residual chlorine comparator |
| CN104034681A (en) * | 2014-06-26 | 2014-09-10 | 南通永康检测技术有限公司 | Method for detecting content of ammonia nitrogen in sewage |
| CN204203105U (en) * | 2014-11-19 | 2015-03-11 | 阜阳师范学院 | Water body ammonia-nitrogen content speed check reagent box |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113391021A (en) * | 2021-03-31 | 2021-09-14 | 赵永刚 | Kit for determining ammonia nitrogen in sample by ion chromatography post-column derivatization method and detection method |
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| CN104374771B (en) | 2018-03-09 |
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Inventor after: Ji Yuntao Inventor after: Qu Changqing Inventor after: Guo Shangfu Inventor before: Ji Yuntao Inventor before: Qu Changqing Inventor before: Jiang Shuanglin Inventor before: He Honghong Inventor before: Lu Juan Inventor before: Du Zhaofeng |
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Effective date of registration: 20180507 Address after: 332100 No. 130 Chai sang Road, Shahe Street Town, Jiujiang County, Jiujiang, Jiangxi. Patentee after: Li Shuangying Address before: 236037 Qinghe West Road, Fuyang, Anhui Province, No. 100 Patentee before: Fuyang Teachers College |
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Granted publication date: 20180309 Termination date: 20181119 |