CN105021599A - Method for detecting ammonia nitrogen in water - Google Patents
Method for detecting ammonia nitrogen in water Download PDFInfo
- Publication number
- CN105021599A CN105021599A CN201410508701.6A CN201410508701A CN105021599A CN 105021599 A CN105021599 A CN 105021599A CN 201410508701 A CN201410508701 A CN 201410508701A CN 105021599 A CN105021599 A CN 105021599A
- Authority
- CN
- China
- Prior art keywords
- ammonia nitrogen
- indicator
- water
- water quality
- detected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to a method for detecting ammonia nitrogen in water. The method for detecting the ammonia nitrogen in water comprises the following steps that S1, a water sample to be detected is used for flushing a 25ml-volume colorimetric tube provided with a stopper and scales for 2-3 times; S2, the water sample to be detected is added into the flushed colorimetric tube, an ammonia nitrogen indicator I and an ammonia nitrogen indicator II are sequentially added and then are shaken evenly; S3, the colorimetric tube is covered with a colorimetric cup, even shaking is performed, standing is performed for 2-3 minutes, the colorimetric tube is attached to the blank position of a colorimetric card, and the ammonia nitrogen of the water sample to be detected can be obtained by being compared with colors of the colorimetric card. The method for detecting the ammonia nitrogen in water has the advantages of being reasonable in design and remarkable in application effect and being operated in a site.
Description
Technical field
The present invention relates to the detection method of ammonia nitrogen in a kind of water quality.
Background technology
Water is vegeto-animal Essential Environment of depending on for existence, not only directly can affect animals and plants existence, also can have influence on vegeto-animal composition, quantity and distribution, and good water environment is the material base ensureing healthy growth of animal plant.The aquatic livestocks such as fish shrimp crab are with water environment for survival, and namely foster fishing supports water, the requirement only having the aspects such as the various physical and chemical index of water and Biological indicators to be all applicable to fishing class to grow, fishing class ability healthy growth.The important water-quality guideline of ammonia-nitrogen content fishing class especially healthy growth in water, it, to the growth, development and fecundity etc. of fishing class, has direct or indirect impact.
Ammonia nitrogen (NH3-N), it directly or indirectly can affect Growth and reproduction and even the death of fish.It directly can be absorbed by plant on the one hand, forms the protein of their life, increases planktonic organism amount, for fish provide abundanter natural bait.On the other hand it to fish and other aquatic animals toxic.It can destroy gill tissue, and infilters in blood, reduces blood oxygen carrying capability, breathing function is declined.
At present, fishermen is more on the one hand judges water quality by rule of thumb by feel, from water sampling process to laboratory assay during another aspect analysis water quality, the time is long, is unfavorable for that fishermen processes fish disease in time, therefore, based on current fishery cultivating present situation, need badly and a kind of convenient, fast and water quality in-situ check and test method that susceptibility is high is provided, namely develop the quick testing product of a kind of ammonia nitrogen water quality, benefiting fishing class aquaculture, will be that this technical field scientific research personnel is badly in need of one of new problem furtheing investigate exploitation.
Summary of the invention
In view of the above-mentioned problems in the prior art, fundamental purpose of the present invention is to address the deficiencies of the prior art, and the invention provides a kind of reasonable in design, execute-in-place and the detection method of ammonia nitrogen in the significant water quality of effect.
The invention provides the detection method of ammonia nitrogen in a kind of water quality, comprise the following steps:
S1: with water sample flushed zone scale to be detected and capacity is the color-comparison tube 2 ~ 3 times of 25ml;
S2: get water sample to be detected and join in above-mentioned color comparison tube after rinsing, ammonium addition indicator I and ammonium addition indicator II successively, then shakes all;
S3: cuvette is covered to described color comparison tube, and shake all, place 2 ~ 3 minutes, color comparison tube is affixed on the blank space of colorimetric card, compares the ammonia nitrogen that can obtain water sample to be detected with colorimetric card color.
Optionally, the preparation method of described ammonia nitrogen indicator I is: taking and analyzing pure sodium potassium tartrate tetrahydrate is 100 ~ 150g, and be settled to 500ml with distilled water, the capacity of being dispensed into is in the ammonia nitrogen indicator bottle I of 30ml, and form ammonia nitrogen indicator I, every bottle is that 25ml is for subsequent use.
Optionally, the preparation method of described ammonia nitrogen indicator II is: take and analyze pure potassium iodide 250g, be uniformly dissolved rear distilled water and be settled to 400ml; Taking and analyzing pure NaOH is 450g, is uniformly dissolved rear distilled water constant volume value 500ml; Weighing developer is that A500g is dissolved in above-mentioned liquor kalii iodide and NaOH solution, and be settled to 2500ml with distilled water after dissolving equilibrium, the capacity of being dispensed into is in the ammonia nitrogen indicator bottle II of 30ml, and form ammonia nitrogen indicator II, every bottle is that 25ml is with for subsequent use.
Optionally, the ammonia nitrogen sensing range of described colorimetric card is 0.20 ~ 1.50mg/L.
The present invention has the following advantages and beneficial effect: utilize different ammonia-nitrogen contents to show different colors to show ammonia nitrogen value from ammonia nitrogen indicator generation chemical reaction, there is the features such as accurate, quick, convenient, inexpensive, without any need for equipment, several methods are adopted to measure; Be applicable to the various field water analyses such as fishery cultivating water, health care, Drinking Water, environmental protection, wastewater recycle, aquarium fish water quality detection, process water, applied range; Using method is very simple, and measurement result is reliable; Volume is little, lightweight, easy to carry; The analytical work of a water sample within 3 ~ 5 minutes, can be completed; Detection analysis cost is low; Tester, without the need to training, only needs by specification step to operate.
Embodiment
Below with reference to specific embodiment, the present invention is further illustrated.
The detection method of ammonia nitrogen in a kind of water quality of the embodiment of the present invention, comprises the following steps:
S1: with water sample flushed zone scale to be detected and capacity is the color-comparison tube 2 ~ 3 times of 25ml;
S2: get water sample to be detected and join in above-mentioned color comparison tube after rinsing, ammonium addition indicator I and ammonium addition indicator II successively, then shakes all;
S3: cuvette is covered to described color comparison tube, and shake all, place 2 ~ 3 minutes, color comparison tube is affixed on the blank space of colorimetric card, compares the ammonia nitrogen that can obtain water sample to be detected with colorimetric card color.
As the preferred implementation of above-described embodiment, the preparation method of described ammonia nitrogen indicator I is: taking and analyzing pure sodium potassium tartrate tetrahydrate is 100 ~ 150g, and be settled to 500ml with distilled water, the capacity of being dispensed into is in the ammonia nitrogen indicator bottle I of 30ml, form ammonia nitrogen indicator I, every bottle is that 25ml is for subsequent use.
As the preferred implementation of above-described embodiment, the preparation method of described ammonia nitrogen indicator II is: take and analyze pure potassium iodide 250g, be uniformly dissolved rear distilled water and be settled to 400ml; Taking and analyzing pure NaOH is 450g, is uniformly dissolved rear distilled water constant volume value 500ml; Weighing developer is that A500g is dissolved in above-mentioned liquor kalii iodide and NaOH solution, and be settled to 2500ml with distilled water after dissolving equilibrium, the capacity of being dispensed into is in the ammonia nitrogen indicator bottle II of 30ml, and form ammonia nitrogen indicator II, every bottle is that 25ml is with for subsequent use.
As the preferred implementation of above-described embodiment, the ammonia nitrogen sensing range of described colorimetric card is 0.20 ~ 1.50mg/L.
In a kind of water quality provided by the invention, the Cleaning Principle of the detection method of ammonia nitrogen is: by colourimetry, utilizes different ammonia-nitrogen contents to show different colors to show ammonia nitrogen value from ammonia nitrogen indicator generation chemical reaction.
The detection method of ammonia nitrogen in a kind of water quality provided by the invention, described ammonia nitrogen indicator bottle I and ammonia nitrogen indicator bottle II all can be used as ammonia nitrogen water quality fast analyser, main agents in described ammonia nitrogen water quality fast analyser provides with working fluid form, easy to use, and reagent is easy to preserve.Different ammonia-nitrogen contents shows different colors to show ammonia nitrogen value from ammonia nitrogen indicator generation chemical reaction, just can be determined the ammonia nitrogen of water quality by colourimetry.This reagent device can detect for 100 times, effectively reduces testing cost.Therefore, the present invention efficiently solves the demand that current Water quality ammonia nitrogen detects fast, solves and uses instrument detection waste time and energy and be not suitable for the difficult problems such as on-site measurement.This reagent device has broad application prospects in water quality detection, and has huge economic benefit and social benefit.
The detection method of ammonia nitrogen in a kind of water quality provided by the invention, described ammonia nitrogen water quality fast analyser precision test, concrete operations are:
The fishery cultivating water ammonia nitrogen quick detection reagent device prepared is carried out repeatability, Intermediate precision, reappearance test respectively to test sample.Replica test: in specialized range, designs 10 different ammonia nitrogen solution (spectrophotometric determination), each concentration do 3 parallel, 3 parallel surveys of each concentration 3 times, evaluate by gained measurement result; Intermediate precision is tested: in this laboratory, is prepared the solution of above-mentioned 10 variable concentrations by 3 different analysts with distinct device, each concentration do 3 parallel, 3 parallel surveys of each concentration 3 times, evaluate by gained measurement result; And evaluate by the measurement result that obtained measurement result and replica test obtain; Reappearance is tested: measured the solution of above-mentioned 10 variable concentrations by different analysts in 2 different testing laboratories.By replica test, Intermediate precision test, the reappearance test test findings evaluation that obtains, test findings is consistent, shows that fishery cultivating water ammonia nitrogen water quality quick detection reagent device precision provided by the present invention reaches requirement.
The detection method of ammonia nitrogen in a kind of water quality provided by the invention, described ammonia nitrogen water quality fast analyser storage life test is as follows:
Test of long duration: the reagent device that three batches prepare is put in 25 DEG C of shady and cool dry places and preserves, measure with a collection of tested water sample respectively at when 0,6,12,18,24 month, result measures and shows that ammonia nitrogen practical measurement value is all within normal color range.Accelerated test: be put in by the reagent device that three batches prepare in 40 DEG C of constant temperature ovens and place 2 weeks, carry out accelerated aging test, then measure with a collection of tested water sample, result shows to treat that in test sample, ammonia nitrogen practical measurement value is all within normal color range.
Last it is noted that above-described each embodiment is only for illustration of technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in previous embodiment, or to wherein partly or entirely technical characteristic carry out equivalent replacement; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the scope of various embodiments of the present invention technical scheme.
Claims (4)
1. the detection method of ammonia nitrogen in water quality, is characterized in that, comprise the following steps:
S1: with water sample flushed zone scale to be detected and capacity is the color-comparison tube 2 ~ 3 times of 25ml;
S2: get water sample to be detected and join in above-mentioned color comparison tube after rinsing, ammonium addition indicator I and ammonium addition indicator II successively, then shakes all;
S3: cuvette is covered to described color comparison tube, and shake all, place 2 ~ 3 minutes, color comparison tube is affixed on the blank space of colorimetric card, compares the ammonia nitrogen that can obtain water sample to be detected with colorimetric card color.
2. the detection method of ammonia nitrogen in water quality according to claim 1, it is characterized in that, the preparation method of described ammonia nitrogen indicator I is: taking and analyzing pure sodium potassium tartrate tetrahydrate is 100 ~ 150g, 500ml is settled to distilled water, the capacity of being dispensed into is in the ammonia nitrogen indicator bottle I of 30ml, form ammonia nitrogen indicator I, every bottle is that 25ml is for subsequent use.
3. the detection method of ammonia nitrogen in water quality according to claim 1, it is characterized in that, the preparation method of described ammonia nitrogen indicator II is: take and analyze pure potassium iodide 250g, be uniformly dissolved rear distilled water and be settled to 400ml; Taking and analyzing pure NaOH is 450g, is uniformly dissolved rear distilled water constant volume value 500ml; Weighing developer is that A500g is dissolved in above-mentioned liquor kalii iodide and NaOH solution, and be settled to 2500ml with distilled water after dissolving equilibrium, the capacity of being dispensed into is in the ammonia nitrogen indicator bottle II of 30ml, and form ammonia nitrogen indicator II, every bottle is that 25ml is with for subsequent use.
4. the detection method of ammonia nitrogen in water quality according to claim 1, it is characterized in that, the ammonia nitrogen sensing range of described colorimetric card is 0.20 ~ 1.50mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410508701.6A CN105021599A (en) | 2014-09-29 | 2014-09-29 | Method for detecting ammonia nitrogen in water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410508701.6A CN105021599A (en) | 2014-09-29 | 2014-09-29 | Method for detecting ammonia nitrogen in water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105021599A true CN105021599A (en) | 2015-11-04 |
Family
ID=54411729
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410508701.6A Pending CN105021599A (en) | 2014-09-29 | 2014-09-29 | Method for detecting ammonia nitrogen in water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105021599A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645123A (en) * | 2016-12-20 | 2017-05-10 | 广东环凯微生物科技有限公司 | Reagent and test paper for rapidly measuring ammonia nitrogen in water, and detection method |
| CN107132216A (en) * | 2016-02-29 | 2017-09-05 | 福建大北农水产科技有限公司 | A kind of breeding water body ammonia nitrogen quick detection kit |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000275234A (en) * | 1999-03-26 | 2000-10-06 | Horiba Ltd | Analyzing method for ammoniacal nitrogen and total nitrogen |
| JP2003014722A (en) * | 2001-07-02 | 2003-01-15 | Meidensha Corp | Ammonia measurement waste liquid treatment method and ammonia measurement apparatus |
| CN101881759A (en) * | 2009-05-05 | 2010-11-10 | 天津生机集团股份有限公司 | Tripolycyanamide rapid detection kit and application thereof |
| CN102393392A (en) * | 2011-10-26 | 2012-03-28 | 天津生机集团股份有限公司 | Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof |
| CN103091148A (en) * | 2013-01-08 | 2013-05-08 | 北京工业大学 | Pretreatment method for measuring ammonia nitrogen in living sewage |
-
2014
- 2014-09-29 CN CN201410508701.6A patent/CN105021599A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000275234A (en) * | 1999-03-26 | 2000-10-06 | Horiba Ltd | Analyzing method for ammoniacal nitrogen and total nitrogen |
| JP2003014722A (en) * | 2001-07-02 | 2003-01-15 | Meidensha Corp | Ammonia measurement waste liquid treatment method and ammonia measurement apparatus |
| CN101881759A (en) * | 2009-05-05 | 2010-11-10 | 天津生机集团股份有限公司 | Tripolycyanamide rapid detection kit and application thereof |
| CN102393392A (en) * | 2011-10-26 | 2012-03-28 | 天津生机集团股份有限公司 | Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof |
| CN103091148A (en) * | 2013-01-08 | 2013-05-08 | 北京工业大学 | Pretreatment method for measuring ammonia nitrogen in living sewage |
Non-Patent Citations (3)
| Title |
|---|
| 国家环境保护局: "《GB 7479-87 水质 铵的测定 纳氏试剂比色法》", 1 August 1987 * |
| 张仁志: "《环境综合实验》", 31 January 2007, 中国环境科学出版社 * |
| 环境保护部: "《HJ 535-2009 水质 氨氮的测定 纳氏试剂分光光度法》", 31 March 2010, 中国环境出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107132216A (en) * | 2016-02-29 | 2017-09-05 | 福建大北农水产科技有限公司 | A kind of breeding water body ammonia nitrogen quick detection kit |
| CN106645123A (en) * | 2016-12-20 | 2017-05-10 | 广东环凯微生物科技有限公司 | Reagent and test paper for rapidly measuring ammonia nitrogen in water, and detection method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cizdziel et al. | Determination of total mercury in human hair and animal fur by combustion atomic absorption spectrometry | |
| Zhao et al. | Nitrate assay for plant tissues | |
| CN102393392A (en) | Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof | |
| CN105651748A (en) | Method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms | |
| CN102507553A (en) | Rapid detection kit of nitrite nitrogen in aquiculture water and detection method using same | |
| CN101825594B (en) | Method for quick nondestructive detection of freshness of freshwater fish | |
| de Moura et al. | Bioenergetic analysis of intact mammalian cells using the Seahorse XF24 Extracellular Flux analyzer and a luciferase ATP assay | |
| CN101699283A (en) | Intelligent food safety detection system and detection method | |
| CN102539640A (en) | Method used for testing high throughput acute toxicity of early life stage of fish | |
| CN102313735A (en) | Kit for rapidly detecting pH value of water for fishery culture and detection method thereof | |
| CN106442515A (en) | Simple and low-cost silver ion visual quantitative detection method | |
| Brown et al. | Evaluation and use of the Lactate Pro, a portable lactate meter, in monitoring the physiological well-being of farmed Atlantic cod (Gadus morhua) | |
| CN105021599A (en) | Method for detecting ammonia nitrogen in water | |
| CN109406504A (en) | Ammonia nitrogen quickly detects solid powder reagent and kit in a kind of water | |
| CN103472194B (en) | Method for measuring and calculating threshold concentration of ecological toxic effect of environmental pollutants | |
| CN105021610A (en) | Method for detecting pH value of water | |
| CN110412022A (en) | A kind of quickly detection organic solid castoff NH3The gel test paper and its application method of volatility | |
| Moodley et al. | Biomass-specific respiration rates of benthic meiofauna: Demonstrating a novel oxygen micro-respiration system | |
| RU2541456C1 (en) | Method for biological assessment of toxicity level of marine environment | |
| CN106018688A (en) | Method for estimating toxicity contribution rate of metal nanoparticle ions and nano effect | |
| CN105021598A (en) | Method for detecting nitrite in water | |
| CN106248898B (en) | The method for detecting water pollution using the total protein content of fish | |
| CN201555840U (en) | Device for detecting safety of foods | |
| Blazier et al. | Assessment of labile organic carbon in soil using sequential fumigation incubation procedures | |
| CN102520078A (en) | Method for determining thiamphenicol and florfenicol residue quantity in culture or slaughter environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151104 |
|
| RJ01 | Rejection of invention patent application after publication |