CN105021598A - Method for detecting nitrite in water - Google Patents
Method for detecting nitrite in water Download PDFInfo
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- CN105021598A CN105021598A CN201410508420.0A CN201410508420A CN105021598A CN 105021598 A CN105021598 A CN 105021598A CN 201410508420 A CN201410508420 A CN 201410508420A CN 105021598 A CN105021598 A CN 105021598A
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Abstract
The invention relates to a method for detecting nitrite in water. The method for detecting the nitrite in water comprises the following steps that S1, a water sample to be detected is used for flushing a 25ml-volume colorimetric tube provided with a stopper and scales for 2-3 times; S2, the water sample to be detected is added into the flushed colorimetric tube till the water sample reaches a scale, 0.08-0.12 g of nitrite indicator is added, and then even shaking is performed; S3, the colorimetric tube is covered with a colorimetric cup, even shaking is performed, standing is performed for 2-3 minutes, the colorimetric tube is attached to the blank position of a colorimetric card, and the nitrite content of the water sample to be detected can be obtained by being compared with colors of the colorimetric card. The method for detecting the nitrite in water has the advantages of being reasonable in design and remarkable in application effect and being operated in a site.
Description
Technical field
The present invention relates to a kind of detection method of water quality nitrite.
Background technology
Water is vegeto-animal Essential Environment of depending on for existence, not only directly can affect animals and plants existence, also can have influence on vegeto-animal composition, quantity and distribution, and good water environment is the material base ensureing healthy growth of animal plant.The aquatic livestocks such as fish shrimp crab are with water environment for survival, and namely foster fishing supports water, the requirement only having the aspects such as the various physical and chemical index of water and Biological indicators to be all applicable to fishing class to grow, fishing class ability healthy growth.Water nitrite is the important water-quality guideline of fishing class healthy growth, and it, to the growth, development and fecundity etc. of fishing class, has direct or indirect impact.
In aquatic animal breeding process, especially under high-density breeding pattern, nitrite is one of Main Factors of restriction aquatic animal normal growth, this is due to the increase along with culturing time, cultivation water nitrite can accumulate gradually, when its concentration reaches certain value, not only can produce fish and directly poison, and various diseases can be brought out, thus have influence on culture benefit.After nitrite poisoning, the oxygen carrying capacity of blood weakens, and cultivation object is than being easier to the symptom forming similar anoxic.During aquatic animal generation nitrite slow poisoning, symptom is not obvious, and naked eyes are difficult to find out, has a strong impact on the growth of aquatic products.Poisoning darker food ration reduces, hypoergia, and fish body is become thin, and body surface is matt, finally causes death in enormous quantities.Time in water containing nitrite, though in water not anoxic also there will be expiratory dyspnea, the utilization factor of feed reduced, the situation such as poor growth.
At present, fishermen is more on the one hand judges water quality by rule of thumb by feel, from water sampling process to laboratory assay during another aspect analysis water quality, time is long, be unfavorable for that fishermen processes fish disease in time, therefore, based on current fishery cultivating present situation, need badly and a kind of convenient, fast and water quality in-situ check and test method that susceptibility is high is provided, namely the quick testing product of a kind of water quality nitrite is developed, benefiting fishing class aquaculture, will be that this technical field scientific research personnel is badly in need of one of new problem furtheing investigate exploitation.
Summary of the invention
In view of the above-mentioned problems in the prior art, fundamental purpose of the present invention is to address the deficiencies of the prior art, and the invention provides a kind of reasonable in design, execute-in-place and the detection method of the significant water quality nitrite of effect.
The invention provides a kind of detection method of water quality nitrite, comprise the following steps:
S1: with water sample flushed zone scale to be detected and capacity is the color-comparison tube 2 ~ 3 times of 25ml;
S2: get water sample to be detected and join to scale mark in the color comparison tube after rinsing, interpolation nitrite indicator 0.08 ~ 0.12g, then shakes all;
S3: cuvette is covered to described color comparison tube, and shake all, place 2 ~ 3 minutes, color comparison tube is affixed on the blank space of colorimetric card, compares the content of nitrite that can obtain water sample to be detected with colorimetric card color.
Optionally, the preparation method of described nitrite indicator is: take and analyze pure citric acid 100 ~ 130g; Take and analyze pure p-aminophenyl sulphonic acid sodium 10 ~ 30g; Take and analyze pure naphthalidine hydrochloride 1 ~ 5g, mixed by above-mentioned three kinds of reagent, the capacity of being dispensed into is that in the nitrite indicator bottle I of 30ml and nitrite indicator bottle II, form nitrite indicator, every bottle of 5g, keeps in Dark Place with for subsequent use.
Optionally, the sensing range of the nitrite number of described colorimetric card is 0.005 ~ 0.30ml/L.
The present invention has the following advantages and beneficial effect: the present invention utilizes nitrite indicator to show nitrite number, has the features such as accurate, quick, convenient, inexpensive, without any need for equipment; Be applicable to the various field water analyses such as fishery cultivating water, health care, Drinking Water, environmental protection, wastewater recycle, aquarium fish water quality detection, process water, applied range; Using method is very simple, and measurement result is reliable; Volume is little, lightweight, easy to carry; The analytical work of a water sample within 3 ~ 5 minutes, can be completed; Detection analysis cost is low; Tester, without the need to training, only needs by specification step to operate.
Embodiment
Below with reference to specific embodiment, the present invention is further illustrated.
The detection method of a kind of water quality nitrite of the embodiment of the present invention, comprises the following steps:
S1: with water sample flushed zone scale to be detected and capacity is the color-comparison tube 2 ~ 3 times of 25ml;
S2: get water sample to be detected and join to scale mark in the color comparison tube after rinsing, interpolation nitrite indicator 0.08 ~ 0.12g, then shakes all;
S3: cuvette is covered to described color comparison tube, and shake all, place 2 ~ 3 minutes, color comparison tube is affixed on the blank space of colorimetric card, compares the content of nitrite that can obtain water sample to be detected with colorimetric card color.
As the preferred implementation of above-described embodiment, the preparation method of described nitrite indicator is: take and analyze pure citric acid 100 ~ 130g; Take and analyze pure p-aminophenyl sulphonic acid sodium 10 ~ 30g; Take and analyze pure naphthalidine hydrochloride 1 ~ 5g, mixed by above-mentioned three kinds of reagent, form nitrite indicator, the capacity of being dispensed into is that in the nitrite indicator bottle of 30ml, every bottle of 5g, keeps in Dark Place with for subsequent use.
As the preferred implementation of above-described embodiment, the sensing range of the nitrite number of described colorimetric card is 0.005 ~ 0.30ml/L.
The Cleaning Principle of the detection method of a kind of water quality nitrite provided by the invention is: nitrite generates diazo salt with sulfanilic acid generation diazo-reaction in acid condition, this diazo salt with alpha-naphthylamine reagent generation coupling reaction, generate aubergine azo-compound.The degree of depth of its color is directly proportional to sample liquid Central Asia nitric acid content, can visual colorimetric determination.
The detection method of a kind of water quality nitrite provided by the invention, described nitrite indicator bottle I and nitrite indicator bottle II all can be used as nitrite water quality fast analyser, main agents in described nitrite water quality fast analyser provides with final product form, easy to use, and reagent is easy to preserve, and this nitrite water quality express-analysis box adopts visual colorimetry, and concentration limit is 0.005mg/L, can detect for 100 times, effectively reduce testing cost.Therefore, the present invention efficiently solves the demand detecting water quality content of nitrite in current aquaculture fast, solves and uses large-scale detector waste time and energy and be not suitable for the difficult problems such as on-site measurement.This nitrite water quality express-analysis box has broad application prospects in field of water quality detection, and has huge economic benefit and social benefit.
The detection method of a kind of water quality nitrite provided by the invention, described nitrite water quality fast analyser precision test, concrete operations are:
The fishery cultivating water nitrite quick detection reagent device prepared is carried out repeatability, Intermediate precision, reappearance test respectively to test sample.Replica test: in specialized range, designs 10 different content of nitrite solution (spectrophotometric determination), each concentration do 3 parallel, 3 parallel surveys of each concentration 3 times, evaluate by gained measurement result; Intermediate precision is tested: in this laboratory, is prepared the solution of above-mentioned 10 variable concentrations by 3 different analysts with distinct device, each concentration do 3 parallel, 3 parallel surveys of each concentration 3 times, evaluate by gained measurement result; And evaluate by the measurement result that obtained measurement result and replica test obtain; Reappearance is tested: measured the solution of above-mentioned 10 variable concentrations by different analysts in 2 different testing laboratories.By replica test, Intermediate precision test, the reappearance test test findings evaluation that obtains, test findings is consistent, shows that fishery cultivating water nitrite water quality quick detection kit precision provided by the present invention reaches requirement.
The detection method of a kind of water quality nitrite provided by the invention, described nitrite water quality fast analyser storage life test is as follows:
Test of long duration: the reagent device that three batches prepare is put in 25 DEG C of shady and cool dry places and preserves, measure with a collection of tested water sample respectively at when 0,6,12,18,24 month, result measures and shows that nitrite practical measurement value is all within normal color range.Accelerated test: be put in by the kit that three batches prepare in 40 DEG C of constant temperature ovens and place 2 weeks, carry out accelerated aging test, then measure with a collection of tested water sample, result shows to treat that test sample nitrite practical measurement value is all within normal color range.
Last it is noted that above-described each embodiment is only for illustration of technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in previous embodiment, or to wherein partly or entirely technical characteristic carry out equivalent replacement; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the scope of various embodiments of the present invention technical scheme.
Claims (3)
1. a detection method for water quality nitrite, is characterized in that, comprises the following steps:
S1: with water sample flushed zone scale to be detected and capacity is the color-comparison tube 2 ~ 3 times of 25ml;
S2: get water sample to be detected and join to scale mark in the color comparison tube after rinsing, interpolation nitrite indicator 0.08 ~ 0.12g, then shakes all;
S3: cuvette is covered to described color comparison tube, and shake all, place 2 ~ 3 minutes, color comparison tube is affixed on the blank space of colorimetric card, compares the content of nitrite that can obtain water sample to be detected with colorimetric card color.
2. the detection method of water quality nitrite according to claim 1, is characterized in that, the preparation method of described nitrite indicator is: take and analyze pure citric acid 100 ~ 130g; Take and analyze pure p-aminophenyl sulphonic acid sodium 10 ~ 30g; Take and analyze pure naphthalidine hydrochloride 1 ~ 5g, mixed by above-mentioned three kinds of reagent, the capacity of being dispensed into is that in the nitrite indicator bottle I of 30ml and nitrite indicator bottle II, form nitrite indicator, every bottle of 5g, keeps in Dark Place with for subsequent use.
3. the detection method of water quality nitrite according to claim 1, is characterized in that, the sensing range of the nitrite number of described colorimetric card is 0.005 ~ 0.30ml/L.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410508420.0A CN105021598A (en) | 2014-09-29 | 2014-09-29 | Method for detecting nitrite in water |
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| CN201410508420.0A CN105021598A (en) | 2014-09-29 | 2014-09-29 | Method for detecting nitrite in water |
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| CN105021598A true CN105021598A (en) | 2015-11-04 |
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| CN201410508420.0A Pending CN105021598A (en) | 2014-09-29 | 2014-09-29 | Method for detecting nitrite in water |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211889A (en) * | 2018-09-21 | 2019-01-15 | 深圳市长隆科技有限公司 | Fast check reagent box of nitrite nitrogen and preparation method thereof and the fast detecting method of nitrite nitrogen |
-
2014
- 2014-09-29 CN CN201410508420.0A patent/CN105021598A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 宋苏顷，夏宁: "对氨基苯磺酸-α-萘胺分光光度法测定水中亚硝酸根方法探讨", 《青岛大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211889A (en) * | 2018-09-21 | 2019-01-15 | 深圳市长隆科技有限公司 | Fast check reagent box of nitrite nitrogen and preparation method thereof and the fast detecting method of nitrite nitrogen |
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Application publication date: 20151104 |
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