CN107132223B - Rapid detection kit for nitrite in aquaculture water - Google Patents
Rapid detection kit for nitrite in aquaculture water Download PDFInfo
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Abstract
The invention discloses a rapid detection kit for nitrite in aquaculture water. The kit contains a bottle of test agent, one colorimetric bottle and one colorimetric card respectively; the test agent comprises sulfanilic acid, naphthyl ethylenediamine hydrochloride, acids, a surfactant, a stabilizer and distilled water; the kit has the advantages of rapidness, sensitivity, convenience in use, stable quality and the like, the problems that the operation steps of a conventional detection kit are complicated in the detection process or the reagent is deteriorated and discolored due to too long time of placement and the like are solved, the operation steps are simple and efficient, any equipment is not required to be added, and the kit is suitable for the field rapid detection of various water qualities.
Description
Technical Field
The invention belongs to the field of water body detection, and particularly relates to a rapid detection kit for nitrite in aquaculture water.
Background
In the intensive aquaculture water, the stocking density of aquatic animals is high, the bait feeding amount is large, a large amount of nitrogen-containing organic matters such as residual baits, animal and plant excreta and dead residues are easily accumulated in the aquaculture water, and in addition, untreated aquaculture wastewater and industrial and domestic sewage are discharged or washed by rainwater to enter the aquaculture water, so that the nitrite in the aquaculture water seriously exceeds the standard.
The overproof nitrite easily causes the breeding of a large amount of harmful bacteria and conditional pathogenic bacteria, the physique of cultured animals such as fish, shrimp and the like is reduced, the stress resistance is poor, the aquatic livestock is easily attacked by pathogenic bacteria, large-area outbreak of aquatic livestock diseases is caused, and huge economic loss is caused to farmers.
Although the common water quality nitrite detection kit in the market is simple and convenient to operate and convenient to carry, the common water quality nitrite detection kit has poor anti-interference capability, is easy to generate false positive and false negative results, and has low sensitivity. Most of the powder is used or a plurality of reagents are used in combination, and farmers often have the problems that the use amount of the powder cannot be controlled, the quality of the reagents deteriorates and the like in the detection process, so that the detection result is inaccurate; sometimes, the used detection reagents are more, the operation is complicated, and larger errors easily occur when the detection result is not noticed slightly. Chinese patent CN 102507553A, CN 103743727A, CN104407097 is a kit for detecting the content of nitrite in aquaculture water, and is formed by mixing common chemical indicators, wherein the reagent has complicated operation steps and poor stability, and the reagent has long color development time.
With the rapid development of the aquaculture industry and the reduction of the breeding risk of farmers, a new nitrite content detection kit with rapid detection, high sensitivity and stable quality is urgently needed.
Disclosure of Invention
In order to solve the problems, the invention provides a rapid detection kit for nitrite in aquaculture water.
The invention provides a rapid detection kit for nitrite in aquaculture water, which comprises a test agent, a colorimetric bottle and a colorimetric card, wherein the test agent is prepared from sulfanilic acid, naphthyl ethylenediamine hydrochloride, acids, a surfactant, a stabilizer and distilled water.
The testing agent is prepared from 1-100 parts by weight of sulfanilic acid, 1-100 parts by weight of naphthyl ethylenediamine hydrochloride, 10-100 parts by weight of acids, 1-50 parts by weight of surfactant, 1-50 parts by weight of stabilizer and 50-100 parts by weight of distilled water.
Further, the test agent is prepared from 10-80 parts by weight of sulfanilic acid, 10-80 parts by weight of naphthyl ethylenediamine hydrochloride, 20-90 parts by weight of acids, 5-40 parts by weight of surfactant, 5-40 parts by weight of stabilizer and 55-100 parts by weight of distilled water.
Furthermore, the test agent is prepared from 15-50 parts by weight of sulfanilic acid, 20-40 parts by weight of naphthyl ethylenediamine hydrochloride, 50-85 parts by weight of acids, 15-30 parts by weight of surfactant, 10-25 parts by weight of stabilizer and 60-90 parts by weight of distilled water.
Wherein, the acid is one or more of hydrochloric acid, acetic acid, citric acid, phosphoric acid and tartaric acid.
Wherein the surfactant is one or more of cetyl trimethyl ammonium bromide, octadecyl trimethyl ammonium bromide (STAB), and cetyl pyridine bromide.
Wherein the stabilizer is one or more of disodium ethylene diamine tetraacetate, monoethanolamine, triethanolamine, polyvinyl alcohol and thiourea.
In the invention, the color comparison card is prepared by color development of nitrite standard solutions (0.005, 0.01, 0.05, 0.10, 0.15, 0.20, 0.25 and 0.30mg/L) with different concentration gradients and a test agent. The method for manufacturing the color comparison card in the nitrite determination kit is a conventional technical means in the field.
The invention also provides a method for detecting nitrite in aquaculture water by using the rapid detection kit for nitrite in aquaculture water, which comprises the following steps:
1) rinsing the colorimetric bottle for 2-3 times by using a water sample to be detected;
2) absorbing a water sample to be detected into a colorimetric bottle, and fixing the volume to 15 mL;
3) 5 drops of the test agent are dripped, the colorimetric bottle is covered, the colorimetric bottle can be compared with a standard colorimetric card after shaking up, and a color block with similar color is found, namely the nitrite content in the water sample to be tested.
The invention has the advantages and positive effects that:
1) the invention upgrades the common powder or the product of the cooperative detection of a plurality of reagents on the market into a single aqueous agent detection reagent in an iterative way, which ensures that a raiser is convenient to carry, simpler to operate and more convenient to use.
2) The cationic surfactant is added into the formed azo dye by utilizing the sensitization characteristic of the surfactant added into the test agent, so that the reaction sensitivity is improved.
3) The stabilizer added in the test agent can maintain the physicochemical property of the test agent and the stability of the product quality, and prolong the effective quality guarantee period of the test agent.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
Weighing the following raw materials in parts by weight: 45 parts of sulfanilic acid, 40 parts of naphthyl ethylenediamine hydrochloride, 85 parts of hydrochloric acid, 30 parts of hexadecyl trimethyl ammonium bromide and 25 parts of ethylene diamine tetraacetic acid, and the composition is obtained by fully dissolving 60 parts of distilled water.
Assembling a kit: 1 bottle of test agent, 1 colorimetric bottle and 1 colorimetric card.
The specific operation steps are as follows:
1) rinsing the colorimetric bottle for 2-3 times by using a water sample to be detected;
2) absorbing a water sample to be detected into a colorimetric bottle, and fixing the volume to 15 mL;
3) 5 drops of the test agent are dripped, the colorimetric bottle is covered, the colorimetric bottle can be compared with a standard colorimetric card after shaking up, and a color block with similar color is found, namely the nitrite content in the water sample to be tested.
Example 2
Weighing the following raw materials in parts by weight: 40 parts of sulfanilic acid, 35 parts of naphthyl ethylenediamine hydrochloride, 60 parts of acetic acid, 20 parts of citric acid, 15 parts of hexadecyl trimethyl ammonium bromide, 10 parts of octadecyl trimethyl ammonium bromide (STAB), 10 parts of monoethanolamine and 15 parts of triethanolamine, and the composition is obtained by fully dissolving the components in 85 parts of distilled water.
Assembling a kit: 1 bottle of test agent, 1 colorimetric bottle and 1 colorimetric card.
The specific operation steps are as follows:
1) rinsing the colorimetric bottle for 2-3 times by using a water sample to be detected;
2) absorbing a water sample to be detected into a colorimetric bottle, and fixing the volume to 15 mL;
3) 5 drops of the test agent are dripped, the colorimetric bottle is covered, the colorimetric bottle can be compared with a standard colorimetric card after shaking up, and a color block with similar color is found, namely the nitrite content in the water sample to be tested.
Example 3
Weighing the following raw materials in parts by weight: 25 parts of sulfanilic acid, 30 parts of naphthyl ethylenediamine hydrochloride, 75 parts of acetic acid, 20 parts of hexadecyl trimethyl ammonium bromide and 15 parts of polyvinyl alcohol, and fully dissolving in 80 parts of distilled water to obtain the product.
Assembling a kit: 1 bottle of test agent, 1 colorimetric bottle and 1 colorimetric card.
The specific operation steps are as follows:
1) rinsing the colorimetric bottle for 2-3 times by using a water sample to be detected;
2) absorbing a water sample to be detected into a colorimetric bottle, and fixing the volume to 15 mL;
3) 5 drops of the test agent are dripped, the colorimetric bottle is covered, the colorimetric bottle can be compared with a standard colorimetric card after shaking up, and a color block with similar color is found, namely the nitrite content in the water sample to be tested.
Example 4
Weighing the following raw materials in parts by weight: 15 parts of sulfanilic acid, 20 parts of naphthyl ethylenediamine hydrochloride, 25 parts of acetic acid, 25 parts of citric acid, 5 parts of phosphoric acid, 5 parts of cetylpyridinium bromide, 5 parts of ethylene diamine tetraacetic acid and 5 parts of thiourea, and fully dissolving in 85 parts of distilled water to obtain the product.
Assembling a kit: 1 bottle of test agent, 1 colorimetric bottle and 1 colorimetric card.
The specific operation steps are as follows:
1) rinsing the colorimetric bottle for 2-3 times by using a water sample to be detected;
2) absorbing a water sample to be detected into a colorimetric bottle, and fixing the volume to 15 mL;
3) 5 drops of the test agent are dripped, the colorimetric bottle is covered, the colorimetric bottle can be compared with a standard colorimetric card after shaking up, and a color block with similar color is found, namely the nitrite content in the water sample to be tested.
Test example 1
The nitrite test agents prepared in the embodiments 1, 2, 3 and 4 of the present invention are used to respectively perform effect observation on the prepared nitrite standard solutions with different concentration gradients.
The operation method comprises the following steps:
(1) and (5) rinsing the colorimetric bottle for 2-3 times by using nitrite standard solution.
(2) And (4) sucking the water sample to be detected into the colorimetric bottle by using a suction pipe, and fixing the volume to 15 mL.
(3) And (3) respectively dropwise adding the ammonia nitrogen reagents prepared in the embodiments 1, 2, 3 and 4 to measure 5 drops, covering the colorimetric bottle, shaking uniformly, comparing with a standard colorimetric card, finding out color blocks with similar colors, namely the nitrite content in the water sample to be measured, and recording the reaction time.
(4) Each example was prepared with 3 replicates of each test agent. Meanwhile, the test agents prepared in the four examples are placed for 6 months and 12 months, then the content of the sub-salt in the same water sample is detected, the color of the test agents is compared with that of a standard color comparison card, and the test results are shown in table 1.
TABLE 1 comparison of the effectiveness of the nitrite test agents of the present invention
The test results show that the nitrite content detected by comparing the four embodiments of the invention with the standard colorimetric card is consistent with the nitrite standard solution concentration, wherein the reaction speed of embodiment 3 has more obvious effect.
Test example 2 test of stability and storage life of nitrite test agent of the present invention
Testing of the stability and shelf life of nitrite test agents prepared using the invention in examples 1 and 2:
and (3) accelerated test:
the nitrite test agent prepared in the embodiment 1 of the invention is respectively placed in three constant temperature and humidity incubators, and the temperature in the incubators is respectively 4 ℃, 25 ℃ and 40 ℃; the mixture was kept at 75 ℃ for 2 weeks. 4 ℃ for maintaining the chemical properties of the test agent as a control; the temperature of 25 ℃ is used for simulating normal temperature preservation of samples; 40 ℃ was used for accelerated testing as an environmentally destructive sample. Then, the same batch of culture water samples are detected, the detection result is compared with 4 ℃, and the detection result of the nitrite at 25 ℃ and 40 ℃ is consistent, which shows that the measured values of the nitrite test agent prepared in the embodiment 1 are all in the normal color development range, and the test agent has good stability.
And (3) long-term test:
testing of the shelf life effect of the nitrite test agent prepared in example 2 of the present invention: and (3) storing a batch of detection kits prepared at the same time in the dark at room temperature. After 2 months, 4 months, 6 months, 8 months, 10 months and 12 months respectively, 6 groups of detection kits in random extraction simultaneously carry out nitrite content determination on a plurality of groups of water samples, simultaneously, the newly prepared reagent at the time point is used for determining the same water sample, the detection results of the kit stored for a period of time and the newly prepared reagent on each group of water samples show consistency, and the detection results are shown in table 2.
Table 2 test results of water sample detection using nitrite test agent prepared in example 2 of the present invention
The experimental result shows that the nitrite test agent prepared in the embodiment 2 of the invention can not deteriorate within one year, and the detection result of the reagent stored for a long time can also reach the standard requirement.
Test example 3
The reaction speed of the nitrite test agent prepared in the embodiment 3 of the invention is compared with that of other nitrite test agents for observation.
And (3) carrying out comparison test on reaction speed of the nitrite detection kit: the group 1 is the nitrite test agent prepared in the embodiment 3, the second group is the nitrite detection reagent group 2 (prepared according to the Chinese patent CN 104407097A), and the third group is the nitrite detection reagent group 3 (prepared according to the Chinese patent CN 103743727), and the nitrite content detection is carried out on the same water sample in Zhao' an farm, and the reaction time of the reagents is recorded. The results are shown in Table 3.
TABLE 3 comparison of reaction times of nitrite test agent detection water samples prepared in example 3 of the present invention
The test result shows that the reaction speed of the nitrite test reagent prepared in the embodiment 3 of the invention is obviously superior to the effect of the nitrite detection reagents in the groups 2 and 3.
Test example 4
The stability of the nitrite test agent prepared in the embodiment 4 of the invention is compared with that of other nitrite test agents for observation.
Stability contrast test of the novel nitrite detection kit: the first group of the nitrite test reagent prepared in the embodiment 4 is used for placing for 6 months and 12 months in the same time period, the second group of the nitrite detection reagent (prepared according to the Chinese patent CN 104407097A), the third group of the nitrite detection reagent (prepared according to the Chinese patent CN 103743727) and the fourth group of the nitrite detection reagent are used for detecting the nitrite content according to GB7493-87 for comparison, the nitrite content detection is carried out on the same water sample of Zhao' an farm, the color change is recorded, and the result is shown in the table 4
TABLE 4 test results of color change detection of nitrite test agent prepared in example 4 of the present invention
The test result shows that the nitrite content of the water sample detected by comparing the nitrite test agent prepared in the embodiment 4 of the invention is consistent with that of the water sample detected by GB7493-87, and the stability and the storage time of the test agent are obviously superior to the detection effects of the nitrite detection agents of the second group and the third group.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A kit for rapidly detecting nitrite in a culture water body comprises a test agent, a colorimetric bottle and a colorimetric card, wherein the test agent is prepared from sulfanilic acid, naphthyl ethylenediamine hydrochloride, acids, a surfactant, a stabilizer and distilled water; the testing agent is prepared from 1-100 parts by weight of sulfanilic acid, 1-100 parts by weight of naphthyl ethylenediamine hydrochloride, 10-100 parts by weight of acids, 1-50 parts by weight of surfactant, 1-50 parts by weight of stabilizer and 50-100 parts by weight of distilled water; the acid is one or more of hydrochloric acid, acetic acid, citric acid, phosphoric acid and tartaric acid; the surfactant is one or more of cetyl trimethyl ammonium bromide, octadecyl trimethyl ammonium bromide and cetyl pyridine bromide; the stabilizer is one or more of disodium ethylene diamine tetraacetate, monoethanolamine, triethanolamine, polyvinyl alcohol and thiourea.
2. The kit for rapidly detecting nitrite in aquaculture water according to claim 1, wherein the test agent is prepared from, by weight, 10-80 parts of sulfanilic acid, 10-80 parts of naphthyl ethylenediamine hydrochloride, 20-90 parts of acids, 5-40 parts of surfactant, 5-40 parts of stabilizer and 55-100 parts of distilled water.
3. The kit for rapidly detecting nitrite in aquaculture water according to claim 2, wherein the test agent is prepared from, by weight, 15-45 parts of sulfanilic acid, 20-40 parts of naphthyl ethylenediamine hydrochloride, 50-85 parts of acids, 15-30 parts of surfactant, 10-25 parts of stabilizer and 60-90 parts of distilled water.
4. The method for detecting nitrite in aquaculture water by using the rapid nitrite detection kit for aquaculture water of any one of claims 1-3, which comprises the following steps: 1) Rinsing the colorimetric bottle for 2-3 times by using a water sample to be detected; 2) Absorbing a water sample to be detected into a colorimetric bottle, and fixing the volume to 15 mL; 3) 5 drops of the test agent are dripped, the colorimetric bottle is covered, the colorimetric bottle can be compared with a standard colorimetric card after shaking up, and a color block with similar color is found, namely the nitrite content in the water sample to be tested.
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| CN201610111373.5A CN107132223B (en) | 2016-02-29 | 2016-02-29 | Rapid detection kit for nitrite in aquaculture water |
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| CN201610111373.5A CN107132223B (en) | 2016-02-29 | 2016-02-29 | Rapid detection kit for nitrite in aquaculture water |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109211889A (en) * | 2018-09-21 | 2019-01-15 | 深圳市长隆科技有限公司 | Fast check reagent box of nitrite nitrogen and preparation method thereof and the fast detecting method of nitrite nitrogen |
| CN111715312A (en) * | 2019-03-21 | 2020-09-29 | 首都师范大学 | Paper-based micro-fluidic chip for multi-parameter analysis of water quality detection and manufacturing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2011017317A1 (en) * | 2009-08-04 | 2011-02-10 | 3M Innovative Properties Company | Method of detecting oxides of nitrogen |
| KR101204542B1 (en) * | 2011-07-14 | 2012-11-23 | 서울시립대학교 산학협력단 | nitrite concentration measuring method |
| CN102507553A (en) * | 2011-10-26 | 2012-06-20 | 天津生机集团股份有限公司 | Rapid detection kit of nitrite nitrogen in aquiculture water and detection method using same |
| CN103529031B (en) * | 2013-11-01 | 2015-12-02 | 内蒙古农业大学 | A kind of semi-solid type nitrite Quick test tube and preparation method thereof |
| CN104407097A (en) * | 2014-11-28 | 2015-03-11 | 苏州东辰林达检测技术有限公司 | Detection reagent and method of nitrite |
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Address after: 363502 Jindu Industrial Concentration Zone, Zhaoan County, Zhangzhou City, Fujian Province Patentee after: Fujian Dabei nonghuayou Aquatic Technology Group Co.,Ltd. Patentee after: BEIJING DABEINONG TECHNOLOGY GROUP Co.,Ltd. Address before: 363502 Jindu Industrial Concentration Zone, Zhaoan County, Zhangzhou City, Fujian Province Patentee before: FUJIAN DABEINONG FISHERIES SCIENCE & TECHNOLOGY Co.,Ltd. Patentee before: BEIJING DABEINONG TECHNOLOGY GROUP Co.,Ltd. |