CN105807018A - Method for comprehensively evaluating threat of ammoniacal nitrogen on aquatic animals - Google Patents
Method for comprehensively evaluating threat of ammoniacal nitrogen on aquatic animals Download PDFInfo
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- CN105807018A CN105807018A CN201510518353.5A CN201510518353A CN105807018A CN 105807018 A CN105807018 A CN 105807018A CN 201510518353 A CN201510518353 A CN 201510518353A CN 105807018 A CN105807018 A CN 105807018A
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- ammoniacal nitrogen
- nitrogen
- nitrite
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
Abstract
The invention relates to a method for evaluating the threat of aquatic animals, and particularly relates to a method for comprehensively evaluating the threat of ammoniacal nitrogen on the aquatic animals. The method is used for comprehensively evaluating the threat of the ammoniacal nitrogen on the aquatic animals by measuring the sum of the own toxicity of the ammoniacal nitrogen and the toxicity of nitrite in a water body. The method has the advantages that by combination with the toxic transformation forms of the ammoniacal nitrogen in water and respective measurement of the contents thereof, the sum of various toxic compounds transformed by nitrogen element in the water body after the aquatic animals are threatened in the form of the ammoniacal nitrogen is comprehensively evaluated so as to avoid leakage of toxic substances due to evaluation on the safety of the polluted water body in certain single toxic form (ammoniacal nitrogen or nitrite) or inconsistency of biomarkers and toxic mechanism to the toxic substances in the environment in the subsequent processes of screening the biomarkers in environment monitoring and researching the toxic mechanism.
Description
Technical field
The present invention relates to and evaluate the method that aquatic animal is threatened, a kind of method that aquatic animal is threatened by overall merit ammoniacal nitrogen specifically.
Background technology
Present stage it is generally acknowledged that aquatile is played the toxic forms of damaging effect and is mainly non-ionic ammonia by ammonia nitrogen, non-ionic ammonia enters after in aquatile body, produce to significantly affect to enzymatic hydrolysis reaction and membrane stability, show dyspnea, do not ingest, resistance decline, the phenomenon such as convulsions, stupor, affect hydrobiological physiology, biochemical indicator and upgrowth situation, may result in aquaculture organism large quantities of death time serious, cause economic loss.Hydrobiological hazard mechanism is currently not and will be apparent from by ammonia nitrogen, it is generally acknowledged that the ammonia nitrogen of high concentration can replace the potassium ion in organism, impact nerve, NMDA (NMDA) receptor-binding activity is caused substantially to reduce, cause central nervous system flowing into excessive calcium ion and causing cell death (RandallDJ, TsuiTKN.Ammoniatoxicityinfish.MarinePollutionBulletin, 2002,45:17-23.).Also it is believed that, the harm of Fish is similar to nitrite (Jianhua XIE by ammoniacal nitrogen, bang is quiet, Wang Aiwu. the effect in water Contamination Assessment and control of simple analysis ammonia nitrogen, total nitrogen, nitrogen cycle and ammonia nitrogen. Inner Mongol water conservancy .2011.5.34-36.), but there is presently no clear and definite data and support this theory.
Most researcheres are all by the separately research of the toxicity of ammoniacal nitrogen and nitrite, such as (the king such as king, Hu Yibo, Jiang Naicheng. ammoniacal nitrogen, the nitrite nitrogen impact on Macrobrachium rosenbergii immunity relevant enzyme. journal of Zhejiang university (Edition), 2005, 32 (6): 698～705) ammoniacal nitrogen is analyzed, the nitrite nitrogen impact on Macrobrachium rosenbergii immunity relevant enzyme, result shows under the action condition of 1mg/L nonionic ammoniacal nitrogen and 1mg/L cultured water, through 1, 4, after within 7 and 10 days, processing, serum and muscle Phenoloxidase Activities all decrease, the phenoloxidase activity of hepatopancrease then slightly increases；SOD vigour changes process in each tissue is first to increase to reduce afterwards.Hong Meiling (2007) studies the nitrite in water and the ammonia nitrogen poisonous effect to Eriocheir Sinensis, it has been found that acute nitrite exposes to expose with acute ammonia nitrogen and Eriocheir sinensis germling hemolymph Glutamic Acid salt, glutamine, the content of carbamide and the content of hepatopancrease GLN all can be made similar variation tendency occur.As far as we know, there is presently no researcher when studying ammoniacal nitrogen toxicity, ammoniacal nitrogen toxicity and nitrite toxicity are merged research.
Summary of the invention
A kind of method that present invention aim at providing overall merit ammoniacal nitrogen that aquatic animal is threatened.
For achieving the above object, the present invention adopts the technical scheme to be:
A kind of method that aquatic animal is threatened by overall merit ammoniacal nitrogen, to measure the summation of the toxicity of ammonia nitrogen in water body own and nitrite toxicity as the overall merit ammoniacal nitrogen threat method to aquatic animal.
Ammonia nitrogen concentration in described mensuration Marine water, and content of nitrite, with both with for the overall merit ammoniacal nitrogen threat method to aquatic animal.
Advantage for present invention:
Ammoniacal nitrogen in water body except with NH4 +、NH3Outside the form such as-N exists, part ammoniacal nitrogen can be become poisonous NO by the microbial transformation in water body2 -, the bio-toxicity of its generation is very important.The present invention is in conjunction with the ammoniacal nitrogen poisonous reformulations in water, and respectively its content is measured, after overall merit coerces aquatile with ammoniacal nitrogen form, the summation of the various toxic compounds that nitrogen element converts in water body, avoid evaluating the safety of polluted water body with wherein a certain single toxic forms (ammoniacal nitrogen or cultured water), intoxicating material is caused to omit, or in follow-up environmental monitoring biomarker screening and intoxicating Mechanism Study process, biomarker and mechanism of toxication and environment intoxicating material inconsistent.
Accompanying drawing explanation
Under the aeration condition that Fig. 1 provides for the embodiment of the present invention, variable concentrations ammoniacal nitrogen exposes the content of nitrite figure after sea water, and wherein, in figure, different letters represent significant difference (p < 0.05).
Detailed description of the invention
Embodiment
Take three parts of sea water (t=20 DEG C, pH8.0), in every part of sea water, add 1mol/L ammonium chloride, be each configured to NH4 +Concentration respectively 0, the matched group of 0.178mmol/L, 0.892mmol/L (t=20 DEG C, pH8.0), low concentration exposure group and high concentration exposure group.Again three groups are placed in box for breeding, box for breeding is added a cover and abundant aeration, difference water sampling after exposing 1 day and 3 days, measures Ammonia In Sea Water nitrogen concentration, the NH of matched group, low concentration exposure group and high concentration exposure group according to marine monitoring specification GB17378.4-2007 sodium hypobromite oxidizing process3-N concentration respectively reaches 0,0.15mg/L and 0.62mg/L (t=20 DEG C, pH8.0)；Additionally take three parts of water samples again, the nitrous nitrification test kit description method that Bioengineering Research Institute develops is built up according to Nanjing, utilizing the content of nitrite in colorimetric method for determining water sample, computing formula is extension rate × 46 × 10 before content of nitrite (mg/L)=(measuring pipe OD value-blank tube OD value)/(standard pipe OD value-blank tube OD value) × normal concentration (100 μm of ol/L) × test sample-3.All sample standard deviations take 3 parallel sampless, and test result utilizes SPSS13.0 to analyze the one factor analysis of variance one-wayANOVA of software, and between group, p < 0.05 is significant difference.
Experimental result is as shown in Figure 1: after ammonia nitrogen exposes 1 day, the content of nitrite 0.35 ± 0.02mg/L of high concentration group is significantly higher than matched group 0.24 ± 0.03mg/L (p < 0.05)；After ammonia nitrogen exposes 3 days, the content of nitrite of low concentration group 0.32 ± 0.03mg/L and high concentration group 0.40 ± 0.09mg/L is all remarkably higher than matched group 0.25 ± 0.04mg/L (p < 0.05, p < 0.01).
Owing to nitrite is a kind of harmful chemical substance, the content of sea water nitrite should control below 0.3 mg/litre.In sea-farming, if content of nitrite is more than 0.5 mg/litre, the life of the marine fish in aquarium and other invertebratess will be subject to seriously.
By data above it can be seen that sea water be subject to ammoniacal nitrogen pollute after, As time goes on, the content of sea water nitrite also rises.After exposing 3 days, the content of nitrite in two kinds of concentration ammonia nitrogen exposure group water environments alreadys more than the minimum critical concentration 0.3mg/L of sea-farming, and the toxicity of cultivated animals be can not be ignored.
Thus, it can be seen that the toxicity of cultivated animals should be the ammonia nitrogen toxicity summation plus nitrite toxicity own by ammoniacal nitrogen, the two can not separate.
Claims (2)
1. the method that aquatic animal is threatened by an overall merit ammoniacal nitrogen, it is characterised in that: to measure the summation of the toxicity of ammonia nitrogen in water body own and nitrite toxicity as the overall merit ammoniacal nitrogen threat method to aquatic animal.
2. the method by the overall merit ammoniacal nitrogen described in claim 1, aquatic animal threatened, it is characterised in that: ammonia nitrogen concentration in described mensuration Marine water, and content of nitrite, with both with for the overall merit ammoniacal nitrogen threat method to aquatic animal.
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| CN201510518353.5A CN105807018A (en) | 2015-08-21 | 2015-08-21 | Method for comprehensively evaluating threat of ammoniacal nitrogen on aquatic animals |
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| CN201510518353.5A CN105807018A (en) | 2015-08-21 | 2015-08-21 | Method for comprehensively evaluating threat of ammoniacal nitrogen on aquatic animals |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109906981A (en) * | 2019-04-04 | 2019-06-21 | 北部湾大学 | The method for improving Pu Eriocheir circulating water cultivation survival rate by control ammonia nitrogen concentration |
| CN115184433A (en) * | 2022-07-16 | 2022-10-14 | 桂林理工大学 | Method for evaluating toxicity of nitrosodiethylamine based on change of fish body calcium ion flow velocity |
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| CN102972323A (en) * | 2012-09-28 | 2013-03-20 | 汪远 | Method for making seawater ecological ornamental aquarium and seawater ecological ornamental aquarium |
| CN104255632A (en) * | 2014-09-18 | 2015-01-07 | 朱祖阳 | Totally-closed all-transparent intellectualized organic prawn ecological breeding device |
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2015
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Patent Citations (2)
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| CN102972323A (en) * | 2012-09-28 | 2013-03-20 | 汪远 | Method for making seawater ecological ornamental aquarium and seawater ecological ornamental aquarium |
| CN104255632A (en) * | 2014-09-18 | 2015-01-07 | 朱祖阳 | Totally-closed all-transparent intellectualized organic prawn ecological breeding device |
Non-Patent Citations (4)
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| 田晓萍 等: "氨态氮 、亚硝酸盐 、pH 等因子对毛蚶育苗的影响", 《河北渔业》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109906981A (en) * | 2019-04-04 | 2019-06-21 | 北部湾大学 | The method for improving Pu Eriocheir circulating water cultivation survival rate by control ammonia nitrogen concentration |
| CN115184433A (en) * | 2022-07-16 | 2022-10-14 | 桂林理工大学 | Method for evaluating toxicity of nitrosodiethylamine based on change of fish body calcium ion flow velocity |
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Application publication date: 20160727 |
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