CN107132223A - A kind of cultivation water nitrite quick detection kit - Google Patents
A kind of cultivation water nitrite quick detection kit Download PDFInfo
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- CN107132223A CN107132223A CN201610111373.5A CN201610111373A CN107132223A CN 107132223 A CN107132223 A CN 107132223A CN 201610111373 A CN201610111373 A CN 201610111373A CN 107132223 A CN107132223 A CN 107132223A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention discloses a kind of cultivation water nitrite quick detection kit.Contain one bottle of test agent, colorimetric bottle and each one of colorimetric card in the kit；Described test agent includes p-aminobenzene sulfonic acid, hydrochloride naphthodiamide, acids, surfactant, stabilizer and distilled water composition；Many advantages, such as kit has rapid sensitive, easy to use, steady quality, it not only compensate for conventional detection kit complex operation step or the problems such as the rotten discoloration of reagent caused by after a long time in detection process, operating procedure is simply efficient simultaneously, additional any equipment is not needed, it is adaptable to the field quick detection of multiple-quality water.
Description
Technical field
The invention belongs to water body detection field, and in particular to a kind of cultivation water nitrite is quick
Detection kit.
Background technology
In intensive culture water body, aquatic livestock breeding density is high, feeds the abundance of food greatly,
Substantial amounts of residual bait, animals and plants excreta, dead residuum etc. are easily accumulated in breeding water body nitrogenous
Organic matter, in addition, undressed breeding wastewater and industry, sanitary sewage be discharged or by
Rain drop erosion enters breeding water body, causes breeding water body nitrite severe overweight.
Nitrite is exceeded to be easily caused harmful bacteria and conditioned pathogen largely grows, fish, shrimp
Constitution Deng cultivated animals declines, and anti-stress ability is poor, is easily attacked, caused by pathogen
The large area outburst of aquatic animal disease, huge economic loss is caused to raiser.
Common water quality detecting kit of nitrites is although easy to operate in the market, take
Band is convenient, but poor anti jamming capability, false positive, false negative result, sensitivity easily occurs
It is relatively low.And mostly pulvis or plurality of reagents be applied in combination, raiser in detection process often
The problems such as usage amount that can often run into pulvis is uncontrollable, reagent quality is rotten, ties detection
It is really inaccurate；Sometimes the detection reagent used is more, cumbersome, does not note detection knot slightly
Easily there is larger error in fruit.Chinese patent CN 102507553A, CN
103743727A, CN 104407097 is the examination for detecting breeding water body nitrite
Agent box, they are that common chemical indicator is mixed, and the former reagent operation step is numerous
Trivial, reagent stability is poor, and the developing time of both rear reagents is a little long.
With the fast development of culture fishery, the cultivation risk of raiser is reduced, is badly in need of having
A kind of new detection is rapid, sensitivity is high, the content of nitrite detection reagent of steady quality
Box.
The content of the invention
In order to solve the above problems, the present invention provides a kind of cultivation water nitrite quick detection
Kit.
The cultivation water nitrite quick detection kit that the present invention is provided, it contains test
Agent, colorimetric bottle and colorimetric card, described test agent is by p-aminobenzene sulfonic acid, hydrochloric acid naphthalene second two
Amine, acids, surfactant, stabilizer and distilled water are made.
Wherein, described test agent is counted by weight, by 1~100 part of p-aminobenzene sulfonic acid,
1~100 part of hydrochloride naphthodiamide, 10~100 parts of acids, 1~50 part of surfactant, stably
50~100 parts of 1~50 part of agent and distilled water are made.
Further, described test agent is counted by weight, by p-aminobenzene sulfonic acid 10~80
Part, 10~80 parts of hydrochloride naphthodiamide, 20~90 parts of acids, 5~40 parts of surfactant,
55~100 parts of 5~40 parts of stabilizer and distilled water are made.
Further, described test agent is counted by weight, by p-aminobenzene sulfonic acid
15~50 parts, 20~40 parts of hydrochloride naphthodiamide, 50~85 parts of acids, surfactant
60~90 parts of 15~30 parts, 10~25 parts of stabilizer and distilled water are made.
Wherein, described acids is one in hydrochloric acid, acetic acid, citric acid, phosphoric acid, tartaric acid
Plant or a variety of.
Wherein, described surfactant is cetyl trimethylammonium bromide, cetyl
Base trimethyl ammonium, Cetyltrimethylammonium bromide (STAB), brocide
In one or more.
Wherein, described stabilizer be disodium ethylene diamine tetraacetate, MEA, triethanolamine,
One or more in polyvinyl alcohol, thiocarbamide.
In the present invention, colorimetric card is the nitrite standard liquid of the various concentrations gradient by configuring
(0.005,0.01,0.05,0.10,0.15,0.20,0.25,0.30mg/L) and test agent
Colour developing is made.The method for making the colorimetric card in nitrous nitrification kit is this area
Conventional technical means.
The present invention also provides the cultivation water nitrite quick detection kit using the present invention
The method for carrying out cultivation water nitrite detection, it comprises the following steps：
1) use water sample rinse colorimetric bottle to be measured 2~3 times；
2) water sample to be measured is drawn into colorimetric bottle, is settled to 15mL；
3) test agent 5 is added dropwise to drip, covers colorimetric bottle, can be with standard colorimetric after shaking up
Card contrast, it is the content of nitrite in water sample to be measured to find out the similar color lump of color.
Advantages and positive effects of the present invention are：
1) present invention changes the product of pulvis common on the market or plurality of reagents cooperation detection
In generation, upgrades to a kind of single aqua detection reagent, and this causes raiser easy to carry, operation
It is simpler, using more facilitate.
2) surfactant added in test agent of the present invention, using its sensitizing property, in shape
Into azo dyes in add cationic surfactant, to improve the sensitivity of reaction.
3) stabilizer added in test agent of the present invention, can maintain test agent physicochemical property,
The stability of product quality, increases effective shelf-life of test agent.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
Each raw material is weighed by mass fraction：45 parts of p-aminobenzene sulfonic acid, hydrochloride naphthodiamide 40
Part, 85 parts of hydrochloric acid, 30 parts of cetyl trimethylammonium bromide, disodium ethylene diamine tetraacetate
25 parts, 60 parts of distilled water are substantially soluble in, are produced.
Kit is assembled：1 bottle of test agent, colorimetric bottle 1 and colorimetric card 1.
Concrete operation step is as follows：
1) use water sample rinse colorimetric bottle to be measured 2~3 times；
2) with water sample to be measured is drawn into colorimetric bottle, it is settled to 15mL；
3) test agent 5 is added dropwise to drip, covers colorimetric bottle, can be with standard colorimetric after shaking up
Card contrast, it is the content of nitrite in water sample to be measured to find out the similar color lump of color.
Embodiment 2
Each raw material is weighed by mass fraction：40 parts of p-aminobenzene sulfonic acid, hydrochloride naphthodiamide 35
Part, 60 parts of acetic acid, 20 parts of citric acid, 15 parts of cetrimonium bromide, 18
10 parts of alkyl trimethyl ammonium bromide (STAB), 10 parts of MEA, 15 parts of triethanolamine,
85 parts of distilled water are substantially soluble in, are produced.
Kit is assembled：1 bottle of test agent, colorimetric bottle 1 and colorimetric card 1.
Concrete operation step is as follows：
1) use water sample rinse colorimetric bottle to be measured 2~3 times；
2) with water sample to be measured is drawn into colorimetric bottle, it is settled to 15mL；
3) test agent 5 is added dropwise to drip, covers colorimetric bottle, can be with standard colorimetric after shaking up
Card contrast, it is the content of nitrite in water sample to be measured to find out the similar color lump of color.
Embodiment 3
Each raw material is weighed by mass fraction：25 parts of p-aminobenzene sulfonic acid, hydrochloride naphthodiamide 30
Part, 75 parts of acetic acid, 20 parts of cetyl trimethylammonium bromide, 15 parts of polyvinyl alcohol, fill
Divide and be dissolved in 80 parts of distilled water, produce.
Kit is assembled：1 bottle of test agent, colorimetric bottle 1 and colorimetric card 1.
Concrete operation step is as follows：
1) use water sample rinse colorimetric bottle to be measured 2~3 times；
2) with water sample to be measured is drawn into colorimetric bottle, it is settled to 15mL；
3) test agent 5 is added dropwise to drip, covers colorimetric bottle, can be with standard colorimetric after shaking up
Card contrast, it is the content of nitrite in water sample to be measured to find out the similar color lump of color.
Embodiment 4
Each raw material is weighed by mass fraction：15 parts of p-aminobenzene sulfonic acid, hydrochloride naphthodiamide 20
Part, 25 parts of acetic acid, 25 parts of citric acid, 5 parts of phosphoric acid, 5 parts of brocide,
5 parts of disodium ethylene diamine tetraacetate, 5 parts of thiocarbamide, are substantially soluble in 85 parts of distilled water, produce.
Kit is assembled：1 bottle of test agent, colorimetric bottle 1 and colorimetric card 1.
Concrete operation step is as follows：
1) use water sample rinse colorimetric bottle to be measured 2~3 times；
2) with water sample to be measured is drawn into colorimetric bottle, it is settled to 15mL；
3) test agent 5 is added dropwise to drip, covers colorimetric bottle, can be with standard colorimetric after shaking up
Card contrast, it is the content of nitrite in water sample to be measured to find out the similar color lump of color.
Test example 1
The Asia prepared using the embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4
Nitrate test agent is imitated to the nitrite titer of the various concentrations gradient of configuration respectively
Fruit is observed.
Operating method is as follows：
(1) nitrite titer rinse colorimetric bottle is used 2~3 times.
(2) water sample to be measured is drawn into colorimetric bottle with suction pipe, be settled to 15mL.
(3) it is added dropwise prepared by embodiment 1, embodiment 2, embodiment 3, embodiment 4 respectively
Ammonia nitrogen reagent surveys each 5 drop, covers colorimetric bottle, can contrast, look for standard color comparison card after shaking up
It is the content of nitrite in water sample to be measured to go out the similar color lump of color, while when recording reaction
Between.
(4) test agent prepared by each embodiment respectively do 3 it is parallel.Simultaneously by four implementations
Test agent prepared by example detects the The sub-salt content with a collection of water sample after placing 6 months, 12 months,
And compared with standard color comparison card progress color, result of the test is shown in Table 1.
The nitrite test agent Contrast on effect of the present invention of table 1
Result of the test shows, is detected after four embodiments of the present invention and standard color comparison card contrast
Content of nitrite and nitrite concentration of standard solution are consistent, and wherein embodiment 3 is in reaction
Effect is more notable in terms of speed.
The experiment of the nitrite test agent stability of the present invention of test example 2 and storage life
Using the embodiment of the present invention 1, embodiment 2 prepare nitrite test agent stability and
The experiment of storage life：
Accelerated test：
Nitrite test agent prepared by the embodiment of the present invention 1 is individually placed to three constant temperature perseverances
In wet incubator, the temperature inside the box is respectively 4 DEG C, 25 DEG C, 40 DEG C；Humidity is 75 DEG C, is put
Put 2 weeks.4 DEG C of chemical characteristics for keeping test agent, are used as control sample；25 DEG C are used for mould
Intend normal temperature and preserve sample；40 DEG C are used for accelerated test, are used as environmental disruption sample.Then
Detection is with a collection of cultivation water sample, and testing result is compareed with 4 DEG C, 25 DEG C, 40 DEG C of nitrite inspections
Survey result unanimously, show the nitrite test agent measured value of the preparation of embodiment 1 normal
Within color range, test agent stability is good.
Long term test：
The examination of the nitrite test agent storage life effect prepared using the embodiment of the present invention 2
Test：The a collection of detection kit that the same time is prepared is placed in lucifuge under room temperature condition and protected
Deposit.Respectively 2 months, 4 months, 6 months, 8 months, 10 months, 12 months it
Afterwards, in randomly selecting 6 groups of detection kits to multigroup water sample while carrying out content of nitrite
Determine, while the reagent newly prepared using the time point is measured to same water sample, preserve
The kit of a period of time is showed the testing result of each group of water sample with the reagent newly configured
Go out uniformity, testing result is shown in Table 2.
Nitrite test agent prepared by the embodiment of the present invention 2 of table 2 detects the result of the test of water sample
Test result indicates that the nitrite test agent that in the present invention prepared by embodiment 2 was in 1 year
It will not go bad in time, the reagent testing result preserved for a long time can equally reach standard
It is required that.
Test example 3
The nitrite test agent prepared using the embodiment of the present invention 3 is examined with other nitrite
The reaction speed paired observation of test agent.
Detecting kit of nitrites reaction speed contrast test：1st group is prepared by embodiment 3
Nitrite test agent, second group be 2 groups of nitrite detection reagent (according to Chinese patent
CN104407097A prepare), the 3rd group be 3 groups of nitrite detection reagent (according to China
It is prepared by patent CN103743727) compare, water sample same to Zhaoan plant carries out sub-
Nitrate content is detected, records the reagent reacting time.It the results are shown in Table 3.
The reaction time contrast of nitrite test agent detection water sample prepared by the embodiment of the present invention 3 of table 3
This result of the test shows that nitrite test agent prepared by the embodiment of the present invention 3 reacts
Speed is substantially better than the effect of the 2nd group and the 3rd group nitrite detection reagent.
Test example 4
The nitrite test agent prepared using the embodiment of the present invention 4 is examined with other nitrite
The stability paired observation of test agent.
New detecting kit of nitrites stability contrast test：Use the placement same time
Section first group of nitrite test agent for the preparation of embodiment 4 of 6 months, 12 months, the
Two groups are nitrite detection reagent (being prepared according to Chinese patent CN104407097A), the
Three groups are nitrite detection reagent (being prepared according to Chinese patent CN103743727), the 4th
Group is compared according to GB7493-87 detection content of nitrite, and to Zhaoan, plant is same
Water sample carries out content of nitrite detection, and record colour developing color change the results are shown in Table 4
Nitrite test agent detection color change result of the test prepared by the embodiment of the present invention 4 of table 4
This result of the test shows that the nitrite prepared by contrasting the embodiment of the present invention 4 is surveyed
The water sample content of nitrite of reagent detection is consistent with GB7493-87 detection content of nitrite,
The stability of its test agent and holding time are substantially better than the nitrite of second group and the 3rd group
The Detection results of detection reagent.
Described above is only the preferred embodiment of the present invention, it is noted that for this technology
For the those of ordinary skill in field, without departing from the technical principles of the invention, also
Some improvements and modifications can be made, these improvements and modifications also should be regarded as the protection of the present invention
Scope.
Claims (8)
1. a kind of cultivation water nitrite quick detection kit, it contains test agent, than
Color bottle and colorimetric card, described test agent by p-aminobenzene sulfonic acid, hydrochloride naphthodiamide, acids,
Surfactant, stabilizer and distilled water are made.
2. cultivation water nitrite quick detection kit according to claim 1,
Characterized in that, described test agent is counted by weight, by p-aminobenzene sulfonic acid 1~100
Part, 1~100 part of hydrochloride naphthodiamide, 10~100 parts of acids, 1~50 part of surfactant,
50~100 parts of 1~50 part of stabilizer and distilled water are made.
3. cultivation water nitrite quick detection kit according to claim 2,
Characterized in that, described test agent is counted by weight, by p-aminobenzene sulfonic acid 10~80
Part, 10~80 parts of hydrochloride naphthodiamide, 20~90 parts of acids, 5~40 parts of surfactant,
55~100 parts of 5~40 parts of stabilizer and distilled water are made.
4. cultivation water nitrite quick detection kit according to claim 3,
Characterized in that, described test agent is counted by weight, by p-aminobenzene sulfonic acid 15~45
Part, 20~40 parts of hydrochloride naphthodiamide, 50~85 parts of acids, 15~30 parts of surfactant,
60~90 parts of 10~25 parts of stabilizer and distilled water are made.
5. the cultivation water nitrite quick detection according to any one of Claims 1 to 4
Kit, it is characterised in that described acids is hydrochloric acid, acetic acid, citric acid, phosphoric acid, wine
One or more in stone acid.
6. the cultivation water nitrite quick detection according to any one of Claims 1 to 4
Kit, it is characterised in that described surfactant is cetyl trimethyl bromination
Ammonium, cetrimonium bromide, Cetyltrimethylammonium bromide, cetyl
One or more in yl pyridines.
7. the cultivation water nitrite quick detection according to any one of Claims 1 to 4
Kit, it is characterised in that described stabilizer is disodium ethylene diamine tetraacetate, monoethanol
One or more in amine, triethanolamine, polyvinyl alcohol, thiocarbamide.
8. utilize the cultivation water nitrite quick detection described in any one of claim 1~7
The method that kit carries out cultivation water nitrite detection, it comprises the following steps：
1) use water sample rinse colorimetric bottle to be measured 2~3 times；
2) water sample to be measured is drawn into colorimetric bottle, is settled to 15mL；
3) test agent 5 is added dropwise to drip, covers colorimetric bottle, can be with standard colorimetric after shaking up
Card contrast, it is the content of nitrite in water sample to be measured to find out the similar color lump of color.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610111373.5A CN107132223B (en) | 2016-02-29 | 2016-02-29 | Rapid detection kit for nitrite in aquaculture water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610111373.5A CN107132223B (en) | 2016-02-29 | 2016-02-29 | Rapid detection kit for nitrite in aquaculture water |
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| Publication Number | Publication Date |
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| CN107132223A true CN107132223A (en) | 2017-09-05 |
| CN107132223B CN107132223B (en) | 2021-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610111373.5A Active CN107132223B (en) | 2016-02-29 | 2016-02-29 | Rapid detection kit for nitrite in aquaculture water |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211889A (en) * | 2018-09-21 | 2019-01-15 | 深圳市长隆科技有限公司 | Fast check reagent box of nitrite nitrogen and preparation method thereof and the fast detecting method of nitrite nitrogen |
| CN111715312A (en) * | 2019-03-21 | 2020-09-29 | 首都师范大学 | Paper-based micro-fluidic chip for multi-parameter analysis of water quality detection and manufacturing method thereof |
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| CN102507553A (en) * | 2011-10-26 | 2012-06-20 | 天津生机集团股份有限公司 | Rapid detection kit of nitrite nitrogen in aquiculture water and detection method using same |
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| WO2011017317A1 (en) * | 2009-08-04 | 2011-02-10 | 3M Innovative Properties Company | Method of detecting oxides of nitrogen |
| KR101204542B1 (en) * | 2011-07-14 | 2012-11-23 | 서울시립대학교 산학협력단 | nitrite concentration measuring method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211889A (en) * | 2018-09-21 | 2019-01-15 | 深圳市长隆科技有限公司 | Fast check reagent box of nitrite nitrogen and preparation method thereof and the fast detecting method of nitrite nitrogen |
| CN111715312A (en) * | 2019-03-21 | 2020-09-29 | 首都师范大学 | Paper-based micro-fluidic chip for multi-parameter analysis of water quality detection and manufacturing method thereof |
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| Publication number | Publication date |
|---|---|
| CN107132223B (en) | 2021-04-02 |
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Address after: 363502 Jindu Industrial Concentration Zone, Zhaoan County, Zhangzhou City, Fujian Province Patentee after: Fujian Dabei nonghuayou Aquatic Technology Group Co.,Ltd. Patentee after: BEIJING DABEINONG TECHNOLOGY GROUP Co.,Ltd. Address before: 363502 Jindu Industrial Concentration Zone, Zhaoan County, Zhangzhou City, Fujian Province Patentee before: FUJIAN DABEINONG FISHERIES SCIENCE & TECHNOLOGY Co.,Ltd. Patentee before: BEIJING DABEINONG TECHNOLOGY GROUP Co.,Ltd. |
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