CN105758995B - The root case and its application method with Rhizosphere DGT tests are cultivated for submerged plant - Google Patents
The root case and its application method with Rhizosphere DGT tests are cultivated for submerged plant Download PDFInfo
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- CN105758995B CN105758995B CN201610118396.9A CN201610118396A CN105758995B CN 105758995 B CN105758995 B CN 105758995B CN 201610118396 A CN201610118396 A CN 201610118396A CN 105758995 B CN105758995 B CN 105758995B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
Abstract
Cultivated and the Rhizosphere DGT root casees tested and its application method for submerged plant the invention provides a kind of, its cabinets cavity is separated into the lower chamber set gradually from the bottom to top by described case using two filter screens, middle level chamber and upper strata chamber, and the root of submerged plant is cultivated in the chamber of middle level, effectively the deposit of Rhizosphere and unrooted deposit are substantially distinguished, the root case apparatus of the present invention is provided with for DGT in the side wall of layer chamber wherein simultaneously, the detection mouth that deposit sampler passes through, DGT is realized to elements such as phosphorus in the original position of plant rhizosphere/Sediment Interface transition process, Accurate Determining, complete plant root feature can also be obtained simultaneously, and the root case of the present invention has simple in construction, plant survival rate is high and can be used for the advantages of first bioavailability such as phosphorus is assessed.The root case application method of the present invention can synchronously realize the cultivation to submerged plant and Rhizosphere DGT in-situ test, and its is easy to operate, is easy to large-scale promotion.
Description
Technical field
The present invention relates to water environment ecological restoration technical field, more particularly to one kind to be used for submerged plant cultivation and plant roots
The root case apparatus and its application method of the element DGT in-situ tests such as border microcell phosphorus.
Background technology
At present, the lake in China existing more than 80% is contaminated, and body eutrophication is on the rise, it has also become current
An overall situation problem urgently to be resolved hurrily, therefore, water body treating arises at the historic moment with water environment ecological reestablishment.According to water body treating
And the difference of Principles, the technology can be divided into Physical, chemical method and bio-ecological process three major types, wherein, biology-raw
State recovery technique is developing progressively as the main of Water Environmental Pollution Control because its cost is low, environmental benefit is high and repairing effect is good
Technological means.In bio-ecological restoration technology, as competitor of the planktonic algae on nutriment and water power calculation, sink
Water plant can effectively suppress the growth of planktonic algae, while can also absorb the nutriments such as the nitrogen in water body, phosphorus, play and decontaminate
Thing, the effect for accelerating organic matter to decompose are contaminated, therefore, the water plant recovery technique based on submerged plant is current this area
Hot research direction.
In the prior art, the method for kind of planting submerged plant mainly has (1) fork planting method：Pitched using the bamboo pole with fork
The firmly neck of submerged plant and fork enters in water；(2) kind of a method is thrown：By the way that manually submerged plant is thrown in water in hydrostatic；(3)
Gradually heavy formula planting method：By submerged plant planting on the carrier, supporting body is controlled heavy by matched elevation and subsidence regulating device
The depth that water plant sinks in water.For above-mentioned implantation methods, because the visibility for being contaminated water body is poor, when people is positioned at shifting
When on dynamic ship, fork planting method and throwing plant method and can not all control the planting density of submerged plant, and the water body flowed exactly
It can also influence to throw the plant position that kind of method is planted, cause plant to produce inclination after sinking under water or even fall on the bottom, from
And influence surviving for submerged plant；And formula planting method is gradually sunk because submerged plant generally cultivates the culture base-material in planting unit bottom
On, the barrier of culture base-material causes the dissolved oxygen amount in water and bed mud near riverbed to reduce, and causes the breeding of anerobe,
Water remediation effect is had a strong impact on, and existing planting unit typically also needs to add mixed bacteria into water body to form biology
Film is repaired to water body, but the purification specific aim of this biomembrane is weak, external source strain poor to the treatment effect of pollutant
Introducing be also unfavorable for water remediation, moreover planting unit is complicated, cost is higher, it is difficult to popularization and application, therefore just compel
Structure improvement will be carried out to the existing formula planting unit that gradually sinks by being essential.
Further, since rhizosphere is crop root carries out nutrient and the important area of energy exchange with soil, it is the micro- life of soil
The most active place of thing, it is that the specific region of the absorption and transhipment of crop nutrition content is influenceed while influenceed again by crop root growth,
Therefore, Rhizosphere is always the focus of the multidisciplinary researches such as domestic and international agrology, Plant Nutrition, agronomy, discloses heavy water and plants
The biological effectiveness of thing Rhizosphere phosphorus transfer and phosphorus is recovered with rebuilding in submerged vegetation the key scientific problems that must be solved,
And the research for this respect at present is still comparatively weak.Although diffusive gradients in thin-films technology (DGT) can be used in soil
Plant root is planted to Transport And Transformation feature between plant rhizosphere/interstitial water/deposit of the absorption dynamics, phosphorus of phosphorus, prediction
The research of the DGT indicators of phosphorus content of Wu Gen areas phosphorus biological effectiveness and plant etc., but so far, DGT is never right
The Rhizosphere of submerged plant carried out test, also failed to for studying mechanism of absorption and biology of the rhizosphere region to elements such as phosphorus
Availability.
It is combined in consideration of it, how to test the DGT of submerged plant Rhizosphere with its root case planting unit, it is straight to realize
It is connected in root case and DGT in-situ tests is carried out to submerged plant Rhizosphere, so as to obtains the elements such as the phosphorus more to tally with the actual situation
The migration feature of rhizosphere region interface and the DGT parameters of root absorption mechanism and each phase for example plant, deposit, interstitial water and
The concentration of element such as the phosphorus in DGT, it is pass of the real grasp submerged plant root area to the element absorptions such as phosphorus and Water Ecological Recovery mechanism
Key, and the still unsolved technical barrier of this research field technical staff.
The content of the invention
Technical problem solved by the invention be to overcome present in existing submerged plant planting device it is complicated,
Water remediation effect is poor, can not carry out the defects of DGT in-situ tests, and then offer one kind is simple in construction, water remediation effect is good
And the submerged plant that is used for that can carry out DGT in-situ tests is cultivated and the Rhizosphere DGT root casees tested and its application method.
Therefore, the present invention realizes that the technical scheme of above-mentioned purpose is：
A kind of root case cultivated for submerged plant with Rhizosphere DGT tests, including：
Casing, the first filter screen and the second filter screen are provided with the casing, first filter screen is positioned at the described second filter
The inner chamber of the casing is separated into and set gradually down from the bottom to top by the top of net, first filter screen and second filter screen
Layer chamber, middle level chamber and upper strata chamber；Wherein,
The open top of the upper strata chamber, set what some stems for submerged plant passed through on first filter screen
Through hole；
The middle level chamber is used for the root for cultivating the submerged plant, and confession is provided with the side wall of the middle level chamber
The detection mouth that DGT, deposit sampler pass through；
The bottom of the lower chamber is provided with the 3rd filter screen.
The ratio between height of the middle level chamber and the lower chamber is 1:1~2:1.
The aperture of first filter screen and the 3rd filter screen is respectively less than the deposit for cultivating the submerged plant
Granularity；First filter screen, the second filter screen and the 3rd filter screen fibreglass are made.
Also include DGT placing devices, the DGT placing devices include conveying lever, distinguish at the both ends of the conveying lever
Fixture and button are set, the spring mechanism for connecting the fixture and button is set along the length direction of the conveying lever.
Also include deposit sampler, it is the plastic injector without syringe needle, for extracting rhizodeposition thing sample；
It is provided for detecting Rhizosphere pH value, Eh values and temperature in the through hole in the middle part of first filter screen
Probe.
Longitudinal scale is set along short transverse outside the side wall of the casing；Along the conveying lever on the conveying lever
Length direction be provided with scale.
Also include a pair of hangers, the pair of hanger is fixedly installed in the relative side wall of the casing top；
Also include closure, the closure is arranged at the outside of the detection mouth.
The application method of above-mentioned case, comprises the following steps：
(1) deposit is added into the lower chamber and the middle level chamber, the root of the submerged plant is planted
In the middle level chamber, and the stem of the submerged plant is set to be extended to through the through hole in the upper strata chamber；
(2) described case is fixed on the floating platform being built in studied waters, makes the distance from top water of described case
0.5~1m of face；
(3) after the submerged plant cultivates a period of time in the floating platform, to the Rhizosphere of the submerged plant
Tested, included the step of the test：
S1, described case is taken out from the floating platform；
S2, will be that the circular DGT for having fine rule is clamped on the fixture of the DGT placing devices, through the detection mouth by institute
State circular DGT to be sent into the rhizodeposition thing of the middle level chamber, it is described so that the circular DGT to be positioned over to press the button
In rhizodeposition thing, the DGT placing devices are taken out, and the end of the fine rule is placed in outside the detection mouth；
S3, the root case for being placed with the circular DGT is fixed in the floating platform again, so that the circular DGT is described
The Rhizosphere of submerged plant carries out in-situ test, after the completion for the treatment of the in-situ test, pulls the fine rule to can be taken off described
Circular DGT.
The deposit comprises the following steps：
The bottom mud in lake that total phosphorus content is 3000mg/Kg is gathered, is air-dried；
The lake sand in not comtaminated waters is gathered, is air-dried；
By the bottom mud in lake after air-drying and lake sand in mass ratio 1:14~14:1 ratio mixes, and produces the deposit.
The distance between the placement location of the circular DGT and the detection mouth are equal to the detection mouth in the described first filter
The distance between online projection and the center of the through hole；
Tested described in step (3) also including the step of being sampled to rhizodeposition thing, be specially：Progress described in
Before step S2, the deposit sampler is inserted in the rhizodeposition thing of the middle level chamber through the detection mouth, extracted
Rhizodeposition thing；
Also include the probe for being used to detect Rhizosphere pH value, Eh values and temperature being inserted into the middle level chamber
In rhizodeposition thing.
The above-mentioned technical proposal of the present invention has the following advantages that：
1st, provided by the present invention for the root case of submerged plant cultivation and Rhizosphere DGT tests, by using two filters
Its cabinets cavity is separated into the lower chamber set gradually from the bottom to top, middle level chamber and upper strata chamber by net, and heavy water is planted
The root of thing is cultivated in the chamber of middle level, so as to which effectively the deposit of Rhizosphere and unrooted deposit substantially be distinguished,
The root case apparatus of the present invention is provided with the detection mouth passed through for DGT, deposit sampler wherein simultaneously in the side wall of layer chamber,
DGT is advantageously implemented to elements such as phosphorus in plant rhizosphere/original position of Sediment Interface transition process, Accurate Determining, and can also
Complete plant root feature is obtained, submerged plant root so just can be more realistically disclosed and the dynamics of the elements such as phosphorus is inhaled
Receipts mechanism and the ecological restoration function to nutrient laden water body in lake.
Also, for the ease of carrying out DGT in-situ tests, it is necessary to which the root case apparatus of the present invention is fixed on floating platform, add again
For true simulation deposit/water/migration of element at plant interface and the purpose of mass exchange, so the present invention is in middle level
The lower section of chamber is provided with lower chamber, and deposit has also been loaded in lower chamber.
2nd, provided by the present invention for the root case of submerged plant cultivation and Rhizosphere DGT tests, by its casing
Longitudinal scale is set along short transverse outside side wall and scale is set along the length direction of conveying lever on conveying lever, with favourable
In accurately determining probe and DGT riding position, so as to more accurately realize the test to submerged plant Rhizosphere.
In addition, the root case apparatus of the present invention also has, simple in construction, plant survival rate is high, water remediation effect is good and can use
In the first bioavailability such as phosphorus is assessed the advantages of.
3rd, the application method of of the present invention case, by the way that the root of the submerged plant is planted in into the middle level chamber
In the deposit of room, and the stem of the submerged plant is set to be extended to through the through hole in the upper strata chamber, while by root
Box top is arranged at 0.5~1m of the water surface, on the one hand can be that surviving for submerged plant provides required illumination and nutrient,
Be advantageous to improve the survival rate of submerged plant, on the other hand avoid the reduction of the dissolved oxygen amount near root case in water environment, and
Without additionally adding strain during due to cultivating submerged plant using the root case of the present invention, so as to ensure submerged plant to water body
Repairing effect.
In addition, the root case application method of the present invention can synchronously realize cultivation to submerged plant and Rhizosphere DGT's
In-situ test, have it is easy to operate, be easy to the advantages of large-scale promotion.
Brief description of the drawings
In order to illustrate more clearly of the technical scheme in the specific embodiment of the invention, embodiment will be retouched below
The required accompanying drawing used is briefly described in stating, it should be apparent that, drawings in the following description are some realities of the present invention
Mode is applied, for those of ordinary skill in the art, on the premise of not paying creative work, can also be attached according to these
Figure obtains other accompanying drawings.
Fig. 1 is the structural representation of the root case apparatus of the present invention；
Fig. 2 is the structural representation of the DGT placing devices in the present invention.
Wherein, reference is as follows：
1- casings；The filter screens of 2- first；The filter screens of 3- second；4- lower chambers；5- middle levels chamber；6- upper stratas chamber；7- through holes；
8- detection mouths；The filter screens of 9- the 3rd；10- conveying levers；11- fixtures；12- buttons；13- circles DGT；14- hangers；15- submerged plants.
Embodiment
Technical scheme is clearly and completely described below in conjunction with accompanying drawing, it is clear that described implementation
Example is part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
In addition, as long as technical characteristic involved in invention described below different embodiments does not form conflict just each other
It can be combined with each other.
Embodiment 1
As shown in figure 1, the submerged plant that is used for described in the present embodiment cultivates the root case tested with Rhizosphere DGT, including：
Casing 1, is provided with the first filter screen 2 and the second filter screen 3 in the casing 1, and first filter screen 2 is positioned at described the
The inner chamber of the casing 1 is separated into from the bottom to top successively by the top of two filter screens 3, first filter screen 2 and second filter screen 3
Lower chamber 4, middle level chamber 5 and the upper strata chamber 6 of setting；Wherein, the open top of the upper strata chamber 6, described first
The through hole 7 that 3 stems for being available for submerged plant pass through is provided with filter screen 1；The middle level chamber 5 is used to cultivate the heavy water plant
The root of thing, the detection mouth 8 passed through for DGT and deposit sampler is provided with the side wall of the middle level chamber 5；Under described
The bottom of layer chamber 4 is provided with the 3rd filter screen 9；In the present embodiment, whole root case is made up of opaque PVC plastic, and it is
One internal diameter is 26cm, a height of 50cm cylinder, and the height of the lower chamber 4 and the middle level chamber 5 is 15cm, described
The aperture of first filter screen 2 and the 3rd filter screen 9 is 28 μm, less than the grain of the deposit for cultivating the submerged plant
Degree, the aperture of second filter screen 3 is 1mm, and first filter screen 2, the second filter screen 3 and the 3rd filter screen 9 in the present embodiment
Fibreglass is made.
Root case apparatus in the present embodiment is by the way that the root of submerged plant is cultivated in the chamber of middle level, effectively by rhizosphere
The soil of microcell is substantially distinguished with unrooted soil, while the root case apparatus of the present invention is provided with the side wall of layer chamber wherein
The detection mouth passed through for DGT, deposit sampler, it is advantageously implemented DGT and the elements such as phosphorus is moved in plant rhizosphere/Sediment Interface
Original position, the Accurate Determining of journey are moved past, submerged plant root so just can be more realistically disclosed and the dynamics of the elements such as phosphorus is inhaled
Receipts mechanism and the ecological restoration function to nutrient laden water body in lake.In addition, the root case apparatus of the present invention also has simple in construction, plant
The advantages of thing survival rate height and good water remediation effect.
The application method of root case in the present embodiment, comprises the following steps：
(1) the lake bottom that total phosphorus content is 3000mg/Kg is gathered in the higher region of Lake Erhai Eutrophic Extent
Mud, air-dry；The lake sand in the preferable waters of unpolluted water quality is gathered, is air-dried；Bottom mud in lake after air-drying is pressed respectively with lake sand
Mass ratio is 1:14~14:1 ratio mixes, and obtains 14 parts of the sequence of deposition thing with total phosphorus concentration gradient；
(2) deposit of step (1) is added into the lower chamber and middle level chamber of described case, each in described case
1 part of deposit is added, the root of spike watermifoil is planted in the middle level chamber, and pass through the stem of spike watermifoil
The through hole is extended in the upper strata chamber；
(3) described case is fixed on and be built on the floating platform that water plant Experimental Base is closed on Erhai, make described case
Distance from top water surface 0.5m；
(4) after spike watermifoil cultivates a growth cycle in the floating platform, to the Rhizosphere of spike watermifoil
Tested, included the step of the test：
S1, described case is taken out from the floating platform；
S2, will be that the circular DGT for having fine rule is clamped on the fixture of the DGT placing devices, through the detection mouth by institute
State circular DGT to be sent into the rhizodeposition thing of the middle level chamber, it is described so that the circular DGT to be positioned over to press the button
In rhizodeposition thing, the DGT placing devices are taken out, the end of the fine rule is placed in outside the detection mouth；
S3, the root case for being placed with the circular DGT is fixed in the floating platform again, so that the circular DGT is in spike
The Rhizosphere of watermifoil carries out in-situ test, after the in-situ test completes (generally requiring 24 hours), pulls described thin
Line can be taken off the circular DGT.
The application method of root case described in the present embodiment, by the way that the root of spike watermifoil is planted in into the middle level chamber
Rhizodeposition thing in, and the stem of spike watermifoil is extended to through the through hole in the upper strata chamber, while by root
Box top is arranged at water surface 0.5m, on the one hand can be the illumination needed for providing that survives of submerged plant spike watermifoil
And nutrient, be advantageous to improve the survival rate of submerged plant, on the other hand avoid the drop of dissolved oxygen amount of the root case nearby in water environment
It is low, and due to need not additionally add strain when cultivating spike watermifoil using the root case of the present invention, so as to ensure spike
Repairing effect of the watermifoil to water body.In addition, the root case application method of the present embodiment can be realized synchronously to spike watermifoil
Cultivate and Rhizosphere DGT in-situ test, have it is easy to operate, be easy to the advantages of large-scale promotion.
Embodiment 2
As shown in figure 1, the submerged plant that is used for described in the present embodiment cultivates the root case tested with Rhizosphere DGT, including：
Casing 1, longitudinal scale is set along short transverse outside the side wall of the casing 1, is provided with the casing 1
First filter screen 2 and the second filter screen 3, first filter screen 2 are located at the top of second filter screen 3, first filter screen 2 and described
The inner chamber of the casing 1 is separated into the lower chamber 4 set gradually from the bottom to top, middle level chamber 5 and upper chamber by the second filter screen 3
Room 6；Wherein,
The open top of the upper strata chamber 6, on first filter screen 1 set 5 be available for the stem of submerged plant to wear
The through hole 7 crossed, and be provided for detecting Rhizosphere pH value, Eh values in the through hole 7 positioned at the middle part of the first filter screen 1
And the probe of temperature；
The middle level chamber 5 is used for the root for cultivating the submerged plant, is provided with the side wall of the middle level chamber 5
The detection mouth 8 passed through for DGT and deposit sampler, the outside of the detection mouth 8 is provided with closure；
The bottom of the lower chamber 4 is provided with the 3rd filter screen 9；
DGT placing devices, Fig. 2, including conveying lever 10 are referred to, along the length of the conveying lever 10 on the conveying lever 10
Degree direction is provided with scale, fixture 11 and button 12 is set respectively at the both ends of the conveying lever 10, along the conveying lever 10
Length direction the spring mechanism for connecting the fixture 11 and button 12 is set；
A pair of hangers 14, the pair of hanger 14 are fixedly installed in the relative side wall at the top of casing 1.
In the present embodiment, whole root case is made up of opaque PVC plastic, its be an internal diameter be 26cm, a height of 50cm
Cylinder, the height of the lower chamber 4 and the middle level chamber 5 is respectively 15cm, 30cm, first filter screen 2 and institute
The aperture for stating the 3rd filter screen 9 is 28 μm, less than the granularity of the deposit for cultivating the submerged plant, the second filter screen 3
Aperture is 1mm, and first filter screen 2, the second filter screen 3 and the fibreglass of the 3rd filter screen 9 in the present embodiment are made.This
The electrode probe (thermoelectricity Ao Lilong companies, the U.S.) and the soil moisture that probe used in embodiment is the portable pH/Eh of 5-Star
The probe (Wuxi Gao Cheng precision instruments Co., Ltd) of data acquisition unit.
The application method of root case in the present embodiment, comprises the following steps：
(1) the lake bottom that total phosphorus content is 3000mg/Kg is gathered in the higher region of Lake Erhai Eutrophic Extent
Mud, air-dry；The lake sand in the preferable waters of unpolluted water quality is gathered, is air-dried；Bottom mud in lake after air-drying is pressed respectively with lake sand
Mass ratio is 14:1~1:14 ratio mixes, and obtains 14 parts of the sequence of deposition thing with total phosphorus concentration gradient；
(2) deposit of step (1) is added into the lower chamber and middle level chamber of described case, each in described case
1 part of deposit is added, the root of spike watermifoil is planted in the middle level chamber, and pass through the stem of spike watermifoil
The through hole is extended in the upper strata chamber；The probe for being used to detect Rhizosphere pH value, Eh values and temperature is inserted
Into the rhizodeposition thing of the middle level chamber；
(3) hawser is set on the pair of hanger 14, described case is fixed on by the hawser and is built in Erhai
On the upper floating platform for closing water plant Experimental Base, make the distance from top water surface 1m of described case；
(4) after spike watermifoil cultivates a growth cycle in the floating platform, to the Rhizosphere of spike watermifoil
Tested, included the step of the test：
S1, described case is taken out from the floating platform, open the closure；
S2, deposit taken sample inserted through the detection mouth in the rhizodeposition thing of the middle level chamber, extract rhizosphere
Deposit, obtain rhizodeposition thing sample；
S3, will be that the circular DGT for having fine rule is clamped on the fixture of the DGT placing devices, through the detection mouth by institute
State circular DGT to be sent into the rhizodeposition thing of the middle level chamber, it is described so that the circular DGT to be positioned over to press the button
In rhizodeposition thing, and ensure that the distance between the placement location of the circular DGT and described detection mouth are equal to the detection mouth
The distance between projection and the center of the through hole on first filter screen；The DGT placing devices are then taken out, by institute
The end for stating fine rule is placed in outside the detection mouth, closes the closure；
S4, the root case for being placed with the circular DGT is fixed on the floating platform again, so that the circular DGT is in spike
The Rhizosphere of watermifoil carries out in-situ test, after the completion for the treatment of the in-situ test, pulls the fine rule to can be taken off the circle
Shape DGT.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
- A kind of 1. application method cultivated for submerged plant with the root case of Rhizosphere DGT tests, it is characterised in that：Described case includes,Casing, is provided with the first filter screen and the second filter screen in the casing, and first filter screen is located at second filter screen The inner chamber of the casing is separated into the lower floor's chamber set gradually from the bottom to top by top, first filter screen and second filter screen Room, middle level chamber and upper strata chamber；Wherein,The open top of the upper strata chamber, some stems for submerged plant are set to pass through on first filter screen logical Hole；The middle level chamber is used to cultivate the root of the submerged plant, be provided with the side wall of the middle level chamber for DGT, The detection mouth that deposit sampler passes through；The bottom of the lower chamber is provided with the 3rd filter screen；DGT placing devices, the DGT placing devices include conveying lever, set respectively at the both ends of the conveying lever fixture and Button, the spring mechanism for connecting the fixture and button is set along the length direction of the conveying lever；The application method comprises the following steps：(1) deposit is added into the lower chamber and the middle level chamber, the root of the submerged plant is planted in institute State in the chamber of middle level, and the stem of the submerged plant is extended to through the through hole in the upper strata chamber；(2) described case is fixed on the floating platform being built in studied waters, makes the distance from top water surface 0.5 of described case ~1m；(3) after the submerged plant cultivates a period of time in the floating platform, the Rhizosphere of the submerged plant is carried out Test, include the step of the test：S1, described case is taken out from the floating platform；S2, will be that the circular DGT for having fine rule is clamped on the fixture of the DGT placing devices, through the detection mouth by the circle Shape DGT is sent into the rhizodeposition thing of the middle level chamber, presses the button so that the circular DGT is positioned over into the rhizosphere In deposit, the DGT placing devices are taken out, and the end of the fine rule is placed in outside the detection mouth；S3, the root case for being placed with the circular DGT is fixed in the floating platform again, so that the circular DGT is in the heavy water The Rhizosphere of plant carries out in-situ test, after the completion for the treatment of the in-situ test, pulls the fine rule to can be taken off the circle DGT。
- 2. the application method of according to claim 1 case, it is characterised in that the middle level chamber and the lower chamber The ratio between height be 1:1~2:1.
- 3. the application method of according to claim 1 case, it is characterised in that first filter screen and the 3rd filter screen Aperture be respectively less than be used for cultivate the submerged plant deposit granularity；First filter screen, the second filter screen and the 3rd filter Net fibreglass is made.
- 4. the application method of the root case according to claim any one of 1-3, it is characterised in that also sampled including deposit Device, it is the plastic injector without syringe needle, for extracting rhizodeposition thing sample；It is provided for detecting the spy of Rhizosphere pH value, Eh values and temperature in the through hole in the middle part of first filter screen Head.
- 5. the application method of according to claim 1 case, it is characterised in that along height outside the side wall of the casing Direction sets longitudinal scale；The length direction of the conveying lever is provided with scale on the conveying lever.
- 6. the application method of according to claim 1 case, it is characterised in that the pair of to hang also including a pair of hangers Ear is fixedly installed in the relative side wall of the casing top；Also include closure, the closure is arranged at the outside of the detection mouth.
- 7. the application method of according to claim 1 case, it is characterised in that the deposit comprises the following steps：The bottom mud in lake that total phosphorus content is 3000mg/Kg is gathered, is air-dried；The lake sand in not comtaminated waters is gathered, is air-dried；By the bottom mud in lake after air-drying and lake sand in mass ratio 1:14~14:1 ratio mixes, and produces the deposit.
- 8. the application method of according to claim 1 case, it is characterised in that the placement location of the circular DGT and institute The distance between detection mouth is stated to be equal between projection and the center of the through hole of the detection mouth on first filter screen Distance.
- 9. the application method of according to claim 4 case, it is characterised in that being tested described in step (3) also includes The step of being sampled to rhizodeposition thing, it is specially：Before the step S2 is carried out, by the deposit sampler through institute State detection mouth to insert in the rhizodeposition thing of the middle level chamber, extract rhizodeposition thing.
- 10. the application method of according to claim 4 case, it is characterised in that also include being used to detect rhizosphere by described The probe of microcell pH value, Eh values and temperature is inserted into the rhizodeposition thing of the middle level chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610118396.9A CN105758995B (en) | 2016-03-02 | 2016-03-02 | The root case and its application method with Rhizosphere DGT tests are cultivated for submerged plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610118396.9A CN105758995B (en) | 2016-03-02 | 2016-03-02 | The root case and its application method with Rhizosphere DGT tests are cultivated for submerged plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105758995A CN105758995A (en) | 2016-07-13 |
| CN105758995B true CN105758995B (en) | 2017-11-21 |
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| CN106489463B (en) * | 2016-09-13 | 2019-05-10 | 中国科学院东北地理与农业生态研究所 | A method of simulation lake protection edaphophyte |
| CN107941990B (en) * | 2017-10-23 | 2020-01-10 | 中国环境科学研究院 | Device and method for testing phosphorus absorption capacity of roots of aquatic plants based on DGT technology |
| CN108519250B (en) * | 2018-04-18 | 2024-02-27 | 中国科学院水生生物研究所 | Device for collecting submerged plant root secretions in situ and use method |
| CN109406244B (en) * | 2018-12-29 | 2023-10-10 | 中国科学院南京地理与湖泊研究所 | Aquatic plant root system secretion normal position collection device |
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| CN101700934A (en) * | 2009-11-17 | 2010-05-05 | 山东建筑大学 | Adjustable recovering method and device of submerged plant in polluted shallow lake |
| CN202272773U (en) * | 2011-08-25 | 2012-06-13 | 上海海洋大学 | Net cage type ecological mattress with adjustable sinking position |
| CN202415267U (en) * | 2011-12-31 | 2012-09-05 | 上海勘测设计研究院 | Suspension submerged plant bed capable of being used at low temperature |
| CN102835310A (en) * | 2012-05-12 | 2012-12-26 | 姜伟 | Double-tray combined type plant tissue culture container |
| CN202881048U (en) * | 2012-09-28 | 2013-04-17 | 中国科学院烟台海岸带研究所 | Multi-medium constructed wetland water treatment system |
| CN103274520A (en) * | 2013-06-19 | 2013-09-04 | 徐州工程学院 | Ecological membrane bioreactor |
| CN103663714A (en) * | 2013-12-21 | 2014-03-26 | 上海海洋大学 | Double-layer net bed for repairing water by planting submerged plants and water repairing method |
| CN103864220B (en) * | 2014-03-28 | 2015-06-17 | 江苏山水环境建设集团股份有限公司 | Multi-step water body ecological purification system and construction method thereof |
| CN103979736B (en) * | 2014-05-21 | 2015-10-28 | 南京大学 | The constructed wetland device of low-pollution water denitrogenation and treatment process thereof |
| CN104322248B (en) * | 2014-10-09 | 2017-06-09 | 吉林省农业科学院 | The Hierarchical Location research method and layering positioner of plant root growth |
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