CN203745032U - Device for measuring plant rhizosphere trace gas during in-situ observation close natural wetland water level fluctuation process - Google Patents
Device for measuring plant rhizosphere trace gas during in-situ observation close natural wetland water level fluctuation process Download PDFInfo
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- CN203745032U CN203745032U CN201420093112.1U CN201420093112U CN203745032U CN 203745032 U CN203745032 U CN 203745032U CN 201420093112 U CN201420093112 U CN 201420093112U CN 203745032 U CN203745032 U CN 203745032U
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Abstract
The utility model discloses a device for measuring plant rhizosphere trace gas during the in-situ observation close natural wetland water level fluctuation process. The device comprises a water level observing area, a buffering area and a testing area, all of which are arranged in sequence and are independent from one another. A drainage system is arranged between the water level observing area and the buffering area, and a drainage system is arranged between the buffering area and the testing area. A root system observing platform is arranged in the testing area. A method for measuring the plant rhizosphere trace gas during the in-situ observation close natural wetland water level fluctuation process comprises the first step of establishing a measuring unit or utilizing a measuring unit established in advance, the second step of cultivating a plant to be measured, the third step of adjusting and controlling the water level, and the fourth step of measuring. The device can provide direct technical support for understanding the dynamic conditions of the dynamic trace gas of an aquatic plant root system-sediment interface.
Description
Technical field
The utility model belongs to field of environment protection; relate to after a kind of lake and river level frequent fluctuation; the device of minimum gas distribution tests in hydrophyte rhizodeposition thing, particularly relates to the device of distribution tests in root system inside-Gen Biao-rhizodeposition thing non-individual body of the secretion oxygen of root system in groundwater level fluctuation process and nitrous oxide.
Background technology
China extends across a plurality of landforms terrace and climate zone, has moulded lake One's name is legion, the overall general layout that distributed more widely, type is complicated.In 2693 lakes, the whole nation, (area is greater than 1.0 km
2) in have such class lake level luffing large (year definitely luffing at 1.70 ~ 15.41 m), reveal the beach phase grow (reaching 305 days most), to reveal beach face long-pending than the large-scale shallow water lake group of great (accounting for 68.9% during maximum), they are mainly distributed in In Middle And Lower Reaches of Changjiang River.For lake regimen, the depth of water is that of statement water level situation effectively measures, and the size of its yardstick also directly reflects lakebed or the sedimental water degree that is subject to.Along with the increase of depth of water gradient, the absorption of influence of hydraulic pressure oxygen and flow, and then limit it and grow by affecting the institutional framework of wetland plant.Meanwhile, depth of water gradient also affects the factor of the habitat of hydrophyte, as sedimental temperature, redox state and light intensity etc. under water, and then affects the distribution of wetland plant.Between depth of water variable effect wetland plant aerial part and under ground portion, comprise the equilibrium relation that biomass between stem and root, Ye Yugen distributes, and nourish and grow and reproductive growth between this balance.SEA LEVEL VARIATION has material impact to lakeside wetland growth and development of plants, and its response on depth of water gradient affects the performance of its ecological environment function and the biogeochemical process of rhizosphere environment thereof.
At present, measuring technology or relevant device or the rarely seen report of relevant water-level fluctuation on root system of plant impact.Method for the monitoring of root system of plant physiological ecological mainly comprises two kinds: a kind of is destructive sampling, directly from the soil body, remove root system of plant, then the way that water rinses obtains the samples such as sediment of root system of plant sample and plant rhizosphere, and then mensuration plant ecophysiological variable, another kind of is indirect method sampling, also by the mode of water planting or husky training, in nutrient solution, cultivate plant, then by organic glass, directly observe the growth of root system and measure plant ecophysiological variable.Above-mentioned destructive sampling technique cannot meet the long-term continuous in-situ observation to root system of plant under water-level fluctuation, and the methods such as water planting and Sha Pei and actual Lakeside Zone actual environment condition difference are larger, the root system activity situation that corresponding achievement can only partial interpretation plant.With the development of this technology, be badly in need of a kind of new equipment of development and technical method, can simulate the nearly natural situation of lakeside wetland, can meet again the continuous in-situ monitoring of water-level fluctuation to root system of plant.
Summary of the invention
The technical problems to be solved in the utility model can be simulated the nearly natural situation of lakeside wetland, can meet again the continuous in-situ monitoring of water-level fluctuation to root system of plant, a kind of continuous in-situ determinator of shallow groundwater lifting process on Root of Wetland Plants impact of observing is provided.
For solving the problems of the technologies described above, the utility model by the following technical solutions:
In the nearly natural wetland the fluctuating procession of the water level of a kind of in-situ observation, measure the device of plant rhizosphere trace gas, comprise the water-level observation district, buffer zone and the test site that set gradually side by side, wherein, described water-level observation district, buffer zone and test site are separate, between Qie water-level observation district and buffer zone, and be equipped with unwatering system between buffer zone and test site; In described test site, be provided with root system observation platform.
According in device of the present utility model, because self metabolism oxygen and nitrous oxide gas and its secreting rate are subject to environmental influence in Root-development, buffer zone be filled with can filter out experimentation in underground water table while regulating external source add water in the filler of the foreign gas such as deliquescent oxygen, nitrous oxide, prevent that it from sneaking into Gen district, test site part.When water level management, in water-level observation district, according to test, need to achieve the goal by the method for adding or remove test water.The test water of adding arrives buffer zone by the unwatering system of bottom of device, and the special physicochemical condition that meets Sediment Pore Water after the filler pre-treatment of buffer zone finally arrives test site by the unwatering system of bottom.Test site growing plants, with the water-level fluctuation root physiology ecosystem characterization also corresponding accommodation of carrying out, some feature of its root system can utilize direct mensuration of test electrode to be fixed on root system of plant corresponding index acquisition data on specimen holder by test site root system observation platform as secreted oxygen speed and denitrification rate.Native system can be realized the Accurate Measurement of root system of plant physiological ecological reaction in the whole dynamic process of water-level fluctuation.
In addition, device of the present utility model also has following additional technical feature:
Preferably, the bottom surface of described water-level observation district, buffer zone and test site is on a surface level.
Preferably, described water-level observation is added with water in district, and the height of described water is-100 ~ 150 cm, is preferably-40 ~ 120cm.Water level management scope-100 ~ 150 cm that the utility model provides (take deposit surface as benchmark, negative value representation level is depth of groundwater table lower than deposit surface).Mostly hydrophyte root growth is within the scope of-80 ~ 120 cm depth of waters, and its root system focuses mostly in the soil layer dark apart from earth's surface 20 ~ 40 cm, so preferred water level management scope is-40 ~ 120cm.
Preferably, this device is made by PVC organic glass or tempered glass is made.
Preferably, described water-level observation district comprises the graduated inspection well of mark and spout hole, and described inspection well is located at the top in described water-level observation district, and described spout hole is located at bottom or the side in described water-level observation district.In experimentation, can carry out by adding the water yield and bottom spout hole balance between the two the height of accurate control observation WIH position.
Preferably, the lower floor of described buffer zone is filled to haydite, and upper strata covers active component and diatomaceous potpourri, and wherein the parts by weight of each component are: active component: zeyssatite: haydite=5 ~ 25 part: 20 ~ 50 parts: 15 ~ 40 parts; When measuring root system of plant oxygen, buffer zone active component is FeSO
4, when measuring root system nitrous oxide, its active component is aluminium oxide.Further preferred, described zeyssatite was 20 ~ 100 order zeyssatite, and haydite is that particle diameter is distributed in 1 ~ 10mm; Preferably, the particle diameter distribution from bottom to up of the haydite of described lower floor gradually from large to small.Active component is selected to change and directed selection with plant root observation index; Its ratio forms to be adjusted with sedimental flooding condition difference.
Preferably, described unwatering system is mainly comprised of two osculums and semipermeable partition, and described osculum diameter is 0.5 ~ 3cm, is located at the identical height in two ends, bottom of described buffer zone, and described semipermeable partition covers on described osculum; Described semipermeable partition allows small organic molecule, solubilised state ion freely to pass through, and does not allow larger molecular organics to pass through; Described semipermeable partition preferably its material is poly (ether sulfone) film or cellulose acetate film.Unwatering system plays overseas Chinese federation's effect in UNICOM test site and water-level observation interval, keeps water-level observation district, the buffer zone flooding condition identical with test site, also consistent with water level, ensures that moisture and dissolved matter are at three region free diffusings simultaneously.
Preferably, described root system observation platform comprises an objective table and some root system geometrical clamps; Described objective table is mainly comprised of circular skeleton and built-in net, and described built-in net covers described skeleton surface, and described root system geometrical clamp is arranged on the surface of described built-in net; Preferably, described framework material generally adopts PVC or stainless steel material to make, and described built-in net adopts nylon wire, white gauze material to make; Preferably, described frame configuration is circular or oval; Preferably, the surface area of described skeleton be bottom section, test site long-pending 1/4 ~ 1/3, bottom, objective table height distance test site 20 ~ 30cm; The circular clip that described root system geometrical clamp is 3 ~ 5mm by 3 ~ 5 diameters forms, clip Surface mulch 3 ~ 5mm thin layer sponge.Hence one can see that, and described its effect of root system geometrical clamp is fixing root system to be measured, and thin layer sponge can be to reduce the physical damnification to root system in clip fixation procedure as far as possible.During test sample, after root system of plant sample spread apart, be laid in built-in on the net, and with root system geometrical clamp, root system fixed.Then according to root system of plant inside-Gen Biao-rhizodeposition object space distribution sequence, measure root system secretion oxygen, nitrous oxide gas-dynamic.
The utility model provides the method for measuring plant rhizosphere trace gas in the nearly natural wetland the fluctuating procession of the water level of a kind of in-situ observation in addition, comprises the following steps:
(1) build or utilize the measuring unit building in advance: setting gradually side by side separate water-level observation district, buffer zone and test site, between water-level observation district and buffer zone, and be equipped with unwatering system between buffer zone and test site; In described test site, be provided with root system observation platform; According to plant root growth condition to be measured, the sediment mixing completely in test site tiling, buffer zone be filled with can filter out experimentation in underground water table while regulating external source add water in the filler of the foreign gas such as deliquescent oxygen, nitrous oxide; In water-level observation, district is filled with water;
(2) cultivate and treat measuring plants;
(3) in incubation, maintain and treat the required water level of measuring plants, as need water level management, in water-level observation district, add or remove test water and reach required height of water level;
(4) while measuring, first in test site, push gently the sediment of root table aside, select radicula that root growth is good as experiment material, and be laid on root system observation platform after being unfolded carefully, and root system is fixed; Then the dissolved oxygen DO pin electrode of correction is utilized the three D controllers moving motor that powers on to be adjusted to reed radicula surface, then sediment is backfilling into root table gently; In water-level observation district regulation and control SEA LEVEL VARIATION, after plant and the stable adaptation of sediment 10 ~ 24h, utilize electro-motor according to the interior method of moving back, the root tip of reed radicula to be divided and carries out puncture test, be specially first the dissolved oxygen pin electrode of puncture is retreated to apart from root table 1cm ~ 2cm left and right scope from Gen Biaonei, then with step-length 20 ~ 50 μ m speed progressively according to the such order of the rhizodeposition thing-Gen Biao-Gen Nei mensuration that punctures, can obtain the distribution characteristics of plant rhizosphere trace gas under different water levels.
The beneficial effects of the utility model: show through repetition test: the method that in the in-situ observation the fluctuating procession of the water level that (1) is provided by the utility model, wetland plant rhizosphere trace gas distributes, observed stage fluctuation situation and root system of plant some eco-physiological indexes change intuitively and accurately.When in device, buffer zone part farthest filters out in experimentation underground water table and regulates, external source is added in water the foreign gases such as deliquescent oxygen, nitrous oxide, prevents that it from sneaking into Gen district, test site part (because in Root-development, self metabolism oxygen and nitrous oxide gas and its secreting rate are subject to environmental influence).(2) utilize the distribution situation that method of the present utility model can this non-individual body trace gas of Accurate Measurement plant root tip position Gen Nei-Gen Biao-rhizodeposition thing.Solve technically the difficult point of root gas original position Accurate Measurement in the fluctuating procession of the water level, to obtaining the heterogeneous ring environment effect of root redox, provide direct evidence.(3) device that the utility model relates to can be understand the dynamic trace gas of hydrophyte root system-Sediment Interface direct technical support is dynamically provided.
The mode that this device blends buffering filtration by bottom of device side is for water-supply source, compare with common unit underground water table control device, reduce the direct water filling in top layer to sedimental percussive action, secondly effective rejecting of the impurity such as effective guarantee oxygen in water in water supply process, makes the gas of the gas that carries in outside water supply and root system self metabolism effectively isolated.The method of testing that the utility model relates in addition meets Root of Wetland Plants trace gas continuously and the requirement of Accurate Measurement, and corresponding method extends to rhizosphere H
2, H
2the monitoring of the materials such as the gas such as S, NO and rhizosphere nitrogen and phosphorous nutrient.
Accompanying drawing explanation
Fig. 1. in the nearly natural wetland the fluctuating procession of the water level of the utility model simulation, measure the system and device structural representation of hydrophyte root system trace gas;
In Fig. 1, each several part is followed successively by: 1. sediment, the 2. skeleton of objective table, 3. built-in net, 4. root system geometrical clamp, 5. haydite, 6. spout hole, 7. zeyssatite and FeSO
4potpourri (or zeyssatite and Al
2o
3), 8. with the inspection well of scale, 9. test site, 10. buffer zone, 11. water-level observation districts, 12. osculums.
When Fig. 2 a. utilizes the utility model system and device in-site detecting reed growing period (60 days) depth of water for-20cm, root tip divides and carries out Gen Nei-Gen Biao in puncture test-rhizodeposition thing oxygen distribution plan;
In Fig. 2 b. Fig. 2 a in root, root table dissolved oxygen concentration distribution plan;
When Fig. 3 a. utilizes the utility model system and device in-site detecting reed growing period (60 days) depth of water to be 40cm, root tip divides and carries out Gen Nei-Gen Biao in puncture test-rhizodeposition thing nitrous oxide distribution plan;
In Fig. 3 b. Fig. 3 a in root, the sub-nitrogen concentration profile figure of root epoxyization.
Embodiment
Below in conjunction with the method that in accompanying drawing and the in-situ observation the fluctuating procession of the water level that the utility model provided by examples of implementation, wetland plant rhizosphere trace gas distributes, be described further, but not as to restriction of the present utility model.
Embodiment 1: in the fluctuating procession of the water level, reed root system dissolved oxygen DO is dynamic
As shown in Figure 1, the present embodiment device used is as follows:
This device is by being made, be separated into water-level observation district 11, the 10He test site, buffer zone 9 setting gradually side by side by PVC organic glass, wherein, described water-level observation district 11, bottom surface separate and three, 10He test site, buffer zone 9 are on a surface level.Between 11He buffer zone, water-level observation district 10, and be equipped with unwatering system between 10He test site, buffer zone 9.Unwatering system is mainly comprised of two osculums 12 and semipermeable partition, and described osculum 12 diameters are 1.5cm, is located at the identical height in two ends, bottom of described buffer zone 10, and described semipermeable partition covers on described osculum 12.Its material of described semipermeable partition is poly (ether sulfone) film, allows small organic molecule, solubilised state ion freely to pass through, and does not allow larger molecular organics to pass through.
In described water-level observation district 11, be added with water, the height of described water is 40 cm
.water-level observation district 11 comprises the graduated inspection well 8 of mark and spout hole 6, and inspection well 8 is located at the top in described water-level observation district 11, and described spout hole 6 is located at the side in described water-level observation district 11.
The lower floor of described buffer zone 10 is filled to haydite 5, and upper strata covers active component and diatomaceous potpourri, and wherein the parts by weight of each component are: active component FeSO
4: zeyssatite: haydite=8 part: 30 parts: 62 parts.Described zeyssatite was 20 ~ 100 order zeyssatite, and haydite is that particle diameter is distributed in 1 ~ 10mm.
In described test site 9, be provided with root system observation platform, root system observation platform comprises an objective table and some root system geometrical clamps 4; Described objective table is mainly comprised of circular skeleton 2 and built-in net 3, and described built-in net 3 covers described skeleton 2 surfaces, and described root system geometrical clamp 4 is arranged on the surface of described built-in net 3; Preferably, described framework material generally adopts PVC or stainless steel material to make, and described built-in net 3 adopts nylon wire to make; Preferably, described skeleton 2 is shaped as circle or ellipse; Preferably, the surface area of described skeleton 2 be 9 bottom sections, test site long-pending 1/3, objective table height distance test site 9 bottom 20cm; The circular clip that described root system geometrical clamp 4 is 5mm by 3 diameters forms, clip Surface mulch 5mm thin layer sponge.
The present embodiment specifically comprises the steps:
(1) experiment material pre-service.First before experiment starts, the packing material of buffer zone 10 is carried out to pre-service, wherein a. haydite cleans and soaks and processes specific practice for first repeatedly to rinse haydite with mobile water, is then placed on and in water, soaks 24h completely.B. zeyssatite is pulverized.Zeyssatite starting material are pulverized, and collect 20 ~ 100 object components standby after sieving.Then according to large particle diameter haydite, fine grained haydite, zeyssatite and FeSO
4the order of potpourri 7 fill the host material of buffer zone 10.When area of observation coverage aqueous sample stream is after buffer zone 10 host materials are processed, filter out and in water sample, carry dissolved oxygen DO, dissolved organic matter etc.Ensure that in the fluctuating procession of the water level, root system of plant is secreted the impact that oxygen activity is mainly limited by flooding condition, and be not subject to that other factors are interactive to be affected.
(2) test site 9 hydrophyte field planting and cultivations.The sediment 60cm that 9 tilings mix completely in test site, after sediment 1 stable in properties, take and be with reed resting bud subterranean stem as brood body field planting.In mid-March, from lakeside wetland, gather Reed rhizome, be cut into the approximately trifle of 2～3cm, resting bud of every trifle band, (planting density is 20 strains/m uniformly-spaced to transplant to test site 9 root system observation platforms
2), make resting bud approximately be positioned at soil table following 5cm place.The germination of reed is carried out under the condition without waterflooding layer, after reed germinating to seedling, progressively pours water to design water level.Plant every other day pours water to maintain water level, and during plant growth, the upper and lower fluctuating error of the depth of water is less than 3cm.
(3) in reed growth, after 60 days, carry out the regulation and control test of Radial Oxygen Loss from Root in the fluctuating procession of the water level.As the distribution of (water level for negative, is just higher than sediment 1 surface lower than sediment 1 surface) reed root system dissolved oxygen DO while measuring water level for-20cm.First 9 sediments 1 of pushing gently root table aside in test site, select radicula that root growth is good as experiment material, and are fixed in carefully on root system observation platform with root system geometrical clamp 4.Then the dissolved oxygen DO pin electrode (tip diameter 10 μ m, Denmark Unisense company) of proofreading and correct is utilized the three D controllers moving motor that powers on to be adjusted to reed radicula surface, then sediment 1 is backfilling into root table gently.In water-level observation district 11 by device middle water level inspection well 8 bottom spout holes 6 regulation and control water levels by original 20cm to-20cm(underground water table lower than the surperficial 40cm of sediment 1), reed and sediment 1 are stable to be adapted to after 24h, utilize electro-motor according to the interior method of moving back, the root tip of reed radicula to be divided and carries out puncture test, be specially first the dissolved oxygen pin electrode of puncture is retreated to apart from root table 1cm ~ 2cm left and right scope from Gen Biaonei, then with step-length 50 μ m speed progressively according to the such order of the rhizodeposition thing-Gen upper surface-Gen Nei-Gen lower surface-rhizodeposition thing mensuration that punctures.Measure the following Fig. 2 of concrete outcome be the depth of water when-20cm, measure tip of a root puncture test process in an oxygen profile figure.
(4) utilize the area of observation coverage interpolation water yield and bottom spout hole 6 to regulate and control different water levels.Water level range can be from-40cm ~ 150cm, and the mensuration of its concrete dissolved oxygen DO is carried out according to step 3. method of this example.After mensuration to be tested finishes, as shown in Fig. 2 a, 2b, can obtain the distribution characteristics of reed root system dissolved oxygen DO under different water levels.
Embodiment 2: in the fluctuating procession of the water level, reed root system nitrous oxide is dynamic
The present embodiment operative installations, with embodiment 1, specifically comprises the steps:
(1) experiment material pre-service.First before experiment starts, the packing material of buffer zone is carried out to pre-service, wherein a. haydite cleans and soaks and processes specific practice for first repeatedly to rinse haydite with mobile water, is then placed on and in water, soaks 24h completely.B. zeyssatite is pulverized.Zeyssatite starting material are pulverized, and collect 20 ~ 100 object components standby after sieving.Then according to large particle diameter haydite, fine grained haydite, Al
2o
3fill the host material of buffer zone with the order of zeyssatite potpourri.By the area of observation coverage, adjust test site water level, when aqueous sample stream that the area of observation coverage adds is after buffer zone host material is processed, filter and in water sample, carry dissolubility inorganic nitrogen, dissolved organic matter etc.Ensure that in the fluctuating procession of the water level, the activity of root system of plant denitrification is mainly limited by the impact of flooding condition, and be not subject to that other factors are interactive to be affected.
(2) field planting of test site hydrophyte and cultivation.The sediment 60cm left and right mixing completely in test site tiling, after sediment properties is stable, take and be with reed resting bud subterranean stem as brood body field planting.In mid-March, from lakeside wetland, gather Reed rhizome, be cut into the approximately trifle of 2～3cm, resting bud of every trifle band, uniformly-spaced transplants that (planting density is 20 strains/m to test site root system observation platform
2), make resting bud approximately be positioned at soil table following 5cm place.The germination of reed is carried out under the condition without waterflooding layer, after reed germinating to seedling, progressively pours water to design water level.Plant every other day pours water to maintain water level, and during plant growth, the upper and lower fluctuating error of the depth of water is less than 3cm.
(5) in reed growth, after 60 days, carry out the regulation and control test of Radial Oxygen Loss from Root in the fluctuating procession of the water level.First gently push the sediment of root table aside, select radicula that root growth is good as experiment material, and be fixed in carefully on root system observation platform with clip.Then the nitrous oxide pin electrode (tip diameter 10 μ m, Denmark Unisense company) of proofreading and correct is utilized the three D controllers moving motor that powers on to be adjusted to reed radicula surface, then sediment is backfilling into root table gently.In water-level observation district by bottom of device spout hole regulation and control water level by original 20cm to 40cm(underground water table higher than water/sediment interface 40cm), reed and sediment are stable to be adapted to after 10 ~ 24h, utilizes micro syringe (to use 10% C to injecting 10% acetylene saturated solution in root table deposited material
2h
2to aeration in deionized water, obtain 10% C with the mixed gas of 90%Air
2h
2saturated solution), sediment is stablized after 2 ~ 3h, utilize electro-motor according to the interior method of moving back, the root tip of reed radicula to be divided and carries out puncture test, be specially first the nitrous oxide pin electrode of puncture retreated to root table 1cm ~ 2cm left and right scope from Gen Biaonei, then with step-length 20 μ m speed progressively according to the such order of the rhizodeposition thing-Gen upper surface-Gen Nei-Gen lower surface-rhizodeposition thing mensuration that punctures.It is that 40cm measures a nitrous oxide profile figure in tip of a root puncture test process at present that the following Fig. 3 of mensuration concrete outcome is the depth of water.
Utilize the area of observation coverage interpolation water yield and bottom spout hole to regulate and control different water levels.Water level range can be from-40cm ~ 150cm, and the mensuration of its concrete dissolved oxygen DO is carried out according to step 3. method of this example.After mensuration to be tested finishes, as shown in Fig. 3 a, 3b, can obtain the distribution characteristics of reed root system dissolved oxygen DO under different water levels.
Claims (7)
1. in the nearly natural wetland the fluctuating procession of the water level of in-situ observation, measure the device of plant rhizosphere trace gas, it is characterized in that, comprise the water-level observation district, buffer zone and the test site that set gradually side by side, wherein, described water-level observation district, buffer zone and test site are separate, between Qie water-level observation district and buffer zone, and be equipped with unwatering system between buffer zone and test site; In described test site, be provided with root system observation platform.
2. device according to claim 1, is characterized in that, the bottom surface of described water-level observation district, buffer zone and test site is on a surface level.
3. device according to claim 1, is characterized in that, described water-level observation is added with water in district, and the height of described water is-100 ~ 150 cm.
4. device according to claim 1, is characterized in that, this device is made by PVC organic glass or tempered glass is made.
5. device according to claim 1, is characterized in that, described water-level observation district comprises the graduated inspection well of mark and spout hole, and described inspection well is located at the top in described water-level observation district, and described spout hole is located at bottom or the side in described water-level observation district.
6. device according to claim 1, it is characterized in that, described unwatering system is mainly comprised of two osculums and semipermeable partition, and described osculum diameter is 0.5 ~ 3cm, the identical height in two ends, bottom that is located at described buffer zone, described semipermeable partition covers on described osculum; Described semipermeable partition allows small organic molecule, solubilised state ion freely to pass through, and does not allow larger molecular organics to pass through; Described semipermeable partition material is poly (ether sulfone) film or cellulose acetate film.
7. device according to claim 1, is characterized in that, described root system observation platform comprises an objective table and some root system geometrical clamps; Described objective table is mainly comprised of circular skeleton and built-in net, and described built-in net covers described skeleton surface, and described root system geometrical clamp is arranged on the surface of described built-in net; Described framework material adopts PVC or stainless steel material to make, and described built-in net adopts nylon wire, white gauze material to make; Described frame configuration is circular or oval; The surface area of described skeleton be bottom section, test site long-pending 1/4 ~ 1/3, bottom, objective table height distance test site 20 ~ 30cm; The circular clip that described root system geometrical clamp is 3 ~ 5mm by 3 ~ 5 diameters forms, clip Surface mulch 3 ~ 5mm thin layer sponge.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103791980A (en) * | 2014-03-03 | 2014-05-14 | 中国科学院南京地理与湖泊研究所 | Device and method for measuring plant rhizosphere trace gases during in-situ close-to-nature wetland water level fluctuation observation |
| CN106489463A (en) * | 2016-09-13 | 2017-03-15 | 中国科学院东北地理与农业生态研究所 | A kind of method of simulation lake protection edaphophyte |
| CN112014544A (en) * | 2020-09-02 | 2020-12-01 | 复旦大学 | A device for in situ research wetland rhizosphere effect |
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2014
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103791980A (en) * | 2014-03-03 | 2014-05-14 | 中国科学院南京地理与湖泊研究所 | Device and method for measuring plant rhizosphere trace gases during in-situ close-to-nature wetland water level fluctuation observation |
| CN103791980B (en) * | 2014-03-03 | 2016-11-23 | 中国科学院南京地理与湖泊研究所 | A kind of in-situ observation near-nature forest wetland the fluctuating procession of the water level is measured the device and method of plant rhizosphere trace gas |
| CN106489463A (en) * | 2016-09-13 | 2017-03-15 | 中国科学院东北地理与农业生态研究所 | A kind of method of simulation lake protection edaphophyte |
| CN106489463B (en) * | 2016-09-13 | 2019-05-10 | 中国科学院东北地理与农业生态研究所 | A method of simulation lake protection edaphophyte |
| CN112014544A (en) * | 2020-09-02 | 2020-12-01 | 复旦大学 | A device for in situ research wetland rhizosphere effect |
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