CN112945691A - Zooplankton sample washing unit - Google Patents

Zooplankton sample washing unit Download PDF

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Publication number
CN112945691A
CN112945691A CN202110155769.0A CN202110155769A CN112945691A CN 112945691 A CN112945691 A CN 112945691A CN 202110155769 A CN202110155769 A CN 202110155769A CN 112945691 A CN112945691 A CN 112945691A
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funnel
cup body
zooplankton
diameter
sample washing
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CN202110155769.0A
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Chinese (zh)
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CN112945691B (en
Inventor
付萍
宋秀凯
姜向阳
何健龙
马元庆
姜会超
刘爱英
刘丽娟
王斌
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Shandong Marine Resource and Environment Research Institute
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Shandong Marine Resource and Environment Research Institute
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34Purifying; Cleaning
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a zooplankton sample washing device, which relates to the technical field of laboratory sample washing devices and comprises an inner funnel, an outer funnel and a funnel cover, wherein the inner funnel is arranged in the outer funnel, and the funnel cover covers the top of the outer funnel; a plurality of side wall holes are formed in the side wall of the cup body of the inner funnel, a filtering device is arranged on the inner wall of the cup body of the inner funnel, and a collecting area is arranged at the bottom of the filtering device; a circulating pipe is arranged on the funnel cover, and the bottom of the circulating pipe extends into the collecting area; and a porous separation plate is arranged above the collection area, a circulation port and a plurality of separation holes are formed in the porous separation plate, and the diameter of the circulation port is matched with that of the circulation pipe and used for enabling the circulation pipe to pass through. The invention can conveniently and quickly filter and collect zooplankton samples.

Description

Zooplankton sample washing unit
Technical Field
The invention relates to the technical field of laboratory sample flushing devices, in particular to a zooplankton sample flushing device.
Background
Zooplankton is a primary consumer in marine ecosystems, controlling phytoplankton density by predation; meanwhile, the zooplankton is also a secondary producer of a marine food chain and is used as a bait organism for high-class organisms such as fishes, so that the zooplankton plays an important role in regulating and controlling the structure and the function of a marine ecosystem. The investigation work of marine zooplankton requires that 5% formaldehyde is added for fixation after trawl sampling, and the microscope is classified and counted in a laboratory. At present, a sample needs to be washed by running water before microscopic examination in a laboratory to remove formaldehyde, the formaldehyde is removed by means of a funnel and a bolting silk, after the filtration, floating swimmers are intercepted on the bolting silk, and then the zooplankters are collected and microscopic examined.
In the washing process, the zooplankton sample to be washed or water flow is easy to splash, so that sample loss and surrounding environment pollution are caused; because no fixing device is arranged, the bolting silk is easy to shift, so that a sample flows out from a gap between the bolting silk and the funnel, and the sample loss is caused; due to the water molecule suction force between the bolting silk and the funnel, the water flow filtering speed is slow, and the time is occupied. In the suction filtration process, zooplankton is retained by the whole bolting-silk, and samples cannot be collected in a centralized manner, so that the samples are easy to lose in the collection process.
Therefore, it is desirable to provide a new zooplankton sample washing device to solve the above problems in the prior art.
Disclosure of Invention
The invention aims to provide a zooplankton sample flushing device, which is used for solving the problems in the prior art and can conveniently and quickly filter and collect zooplankton samples.
In order to achieve the purpose, the invention provides the following scheme: the invention provides a zooplankton sample washing device which comprises an inner funnel, an outer funnel and a funnel cover, wherein the inner funnel is arranged in the outer funnel, and the funnel cover covers the top of the outer funnel; a plurality of side wall holes are formed in the side wall of the cup body of the inner funnel, a filtering device is arranged on the inner wall of the cup body of the inner funnel, and a collecting area is arranged at the bottom of the filtering device; a circulating pipe is arranged on the funnel cover, and the bottom of the circulating pipe extends into the collecting area; and a porous separation plate is arranged above the collection area, a circulation port and a plurality of separation holes are formed in the porous separation plate, and the diameter of the circulation port is matched with that of the circulation pipe and used for enabling the circulation pipe to pass through.
Preferably, interior funnel includes first toper cup and the first cylindrical cup of an organic whole connection, the diameter of first toper cup reduces from top to bottom in proper order, the diameter of first cylindrical cup with the bottom diameter of first toper cup equals, the top of first cylindrical cup with the bottom of first toper cup is connected.
Preferably, a plurality of side wall holes are formed in the side wall of the first conical cup body, and the diameter of each side wall hole is larger than or equal to 2 cm.
Preferably, the filtering device adopts a bolting silk which is attached to the inner wall of the first conical cup body; the top end of the first conical cup body is provided with a fixing clamp for fixing the bolting silk; the fixation clamp is provided with 3 along the top edge of first toper cup equidistant.
Preferably, the outer funnel comprises a second conical cup body and a second cylindrical cup body which are integrally connected, the diameter of the second conical cup body is sequentially reduced from top to bottom, the diameter of the second cylindrical cup body is equal to the diameter of the bottom of the second conical cup body, and the top of the second cylindrical cup body is connected with the bottom of the second conical cup body;
the top outer edge of the first conical cup body is connected with the top inner edge of the second conical cup body through a fixed transverse strip, and the number of the fixed transverse strip is 3 along the top outer edge of the first conical cup body at equal intervals.
Preferably, the outer edge of the top of the first conical cup body, the fixing cross bar and the inner edge of the top of the second conical cup body are connected into a whole.
Preferably, 3 first buckles are arranged on the outer side of the top edge of the second conical cup body at equal intervals, 3 second buckles are correspondingly arranged on the outer side of the cover edge of the funnel cover at equal intervals, and the funnel cover and the second conical cup body are connected through the first buckles and the second buckles.
Preferably, the lower end of the circulation pipe is connected with a transverse plate, and the transverse plate is used for fixing the porous separation plate.
Preferably, the funnel cover, the flow pipe and the transverse plate are integrally connected.
Preferably, the aperture of the separation hole is less than 0.05mm, and the separation hole is used for trapping zooplankton.
Compared with the prior art, the invention has the following beneficial technical effects:
the traditional filtering method is that the bolting silk is only attached to the inner wall of the funnel after being wetted, and the bolting silk is displaced due to unstable placement position or overlarge flushing water flow in a flushing process, so that a sample is easy to flow out from the funnel and the bolting silk;
in the traditional filtering mode, because water molecule attraction exists between the bolting silk and the funnel, the water flow is filtered slowly, and the time is occupied, the invention is provided with two embedded funnels, and the side wall of the inner funnel is provided with dense side wall holes, so that the contact area between the side wall and the bolting silk can be effectively reduced, the molecular attraction is reduced, and the water flow filtering speed is accelerated; meanwhile, the outer funnel connected outside the inner funnel can effectively collect liquid overflowing from the side wall hole of the inner funnel, so that liquid leakage is avoided;
in the traditional filtering process, the glass rod is used for drainage, the bolting silk is easy to puncture by taking carelessness as the operation is laborious, the sample loss is caused, and the monitoring result is inaccurate; the funnel cover connected with the flow pipe can isolate the operator from the suction filtration activity, and prevent water from splashing and polluting the surrounding environment;
in the traditional washing process, zooplankton samples are intercepted by the whole bolting-silk and cannot be collected in a centralized manner, the porous separation plate is arranged, after the samples are drained to the bottom of the bolting-silk, the porous separation plate is arranged on the upper part of the collection area, the flow pipe passes through the flow opening, the porous separation plate is fixed by the transverse plate on the flow pipe, and the separation plate is prevented from moving under the action of buoyancy in the washing process; in the washing process, water flow can directly reach the lower part of the porous separation plate, formaldehyde can be continuously washed and removed from zooplankton samples in the collection area, water molecules can enter and exit through the separation holes, are filtered by the bolting silk and quickly flow out through the side wall holes, and the zooplankton samples can be kept in the collection area to play a role in centralized collection.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a zooplankton sample washing device of the present invention;
FIG. 2 is a schematic structural view of an outer funnel of the present invention;
FIG. 3 is a schematic view of the construction of the inner funnel of the present invention;
FIG. 4 is a schematic view of the construction of the funnel cover of the present invention;
FIG. 5 is a schematic view of the structure of a porous separation plate according to the present invention;
wherein, 1 is interior funnel, 2 is outer funnel, 3 is the funnel lid, 4 is porous separator plate, 5 is the side wall hole, 6 is the fixation clamp, 7 is fixed horizontal bar, 8 is the runner pipe, 9 is first buckle, 10 is the second buckle, 11 is the diaphragm, 12 is first toper cup, 13, first cylindrical cup, 14 is second toper cup, 15 is the cylindrical cup of second, 16 is the runner hole, 17 is the separation hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide a zooplankton sample flushing device, which is used for solving the problems in the prior art and can conveniently and quickly filter and collect zooplankton samples.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Example one
As shown in fig. 1 to 5, the present embodiment provides a zooplankton sample washing device, including an inner funnel 1, an outer funnel 2 and a funnel cover 3, where the inner funnel 1 is installed in the outer funnel 2, and the funnel cover 3 covers the top of the outer funnel 2; the side wall of the cup body of the inner funnel 1 is provided with a plurality of densely arranged side wall holes 5 which can promote the accelerated filtering of water flow, the inner wall of the cup body of the inner funnel 1 is provided with a filtering device, and the bottom of the filtering device is a collecting area; the middle part of the funnel cover 3 is connected with a circulating pipe 8, and the bottom of the circulating pipe 8 extends into the collecting area for drainage; a porous separation plate 4 is arranged above the collection area, a circulation port 16 is arranged in the middle of the porous separation plate 4, dense separation holes 17 are arranged around the circulation port 16, and the diameter of the circulation port 16 is matched with that of the circulation pipe 8, so that the circulation pipe 8 can just pass through the circulation port; the aperture of the separation hole 17 is smaller than 0.05mm, and the separation hole is used for intercepting zooplankton and separating zooplankton samples from water.
In this embodiment, inner funnel 1 includes first toper cup 12 and first cylindrical cup 13 of integrated into one piece, and the diameter of first toper cup 12 reduces from top to bottom in proper order, and the diameter of first cylindrical cup 13 equals with the bottom diameter of first toper cup 12, and the top of first cylindrical cup 13 is connected with the bottom of first toper cup 12. Wherein, a plurality of densely arranged side wall holes 5 are arranged on the side wall of the first conical cup body 12, and the diameter of the side wall holes 5 is not less than 2 cm.
In this embodiment, the filtering device is made of a silk screen, and the silk screen is attached to the inner wall of the first conical cup body 12; the top end of the first conical cup body 12 is provided with a fixing clamp 6 for clamping and fixing the bolting silk; wherein, fixation clamp 6 is provided with 3 along the top edge of first toper cup 12 equidistant, improves fixed effect.
In the embodiment, the outer funnel 2 comprises a second tapered cup body 14 and a second cylindrical cup body 15 which are integrally connected, the diameter of the second tapered cup body 14 is reduced from top to bottom in sequence, the diameter of the second cylindrical cup body 15 is equal to the diameter of the bottom of the second tapered cup body 14, and the top of the second cylindrical cup body 15 is connected with the bottom of the second tapered cup body 14; wherein, the diameter of outer funnel 2 is greater than the diameter of inner funnel 1, and outer funnel 2 sets up in the outside of inner funnel 1 for collect the liquid that inner funnel 1 lateral wall oozed.
In the embodiment, a fixed cross bar 7 is arranged on the outer side of the top end of a first conical cup body 12 of the inner funnel 1, the outer side of the fixed cross bar 7 is connected with a second conical cup body 14 of the outer funnel 2, and the outer edge of the top of the first conical cup body 12 is connected with the inner edge of the top of the second conical cup body 14 through the fixed cross bar 7; wherein, fixed horizontal bar 7 is provided with 3 at equal intervals along the top outer fringe of first toper cup 12. Further, the top outer edge of the first conical cup 12, the fixing cross bar 7 and the top inner edge of the second conical cup 14 are integrally connected.
In this embodiment, 3 first fasteners 9 are provided on the outer side of the top edge of the second cone 14 at equal intervals, 3 second fasteners 10 are provided on the outer side of the cover edge of the funnel cover 3 at equal intervals, and the first fasteners 9 and the second fasteners 10 are combined to realize the connection between the funnel cover 3 and the second cone 14.
In this embodiment, the lower end of the flow tube 8 is connected with a transverse plate 11, and the transverse plate 11 is used for fixing the porous separation plate 4; in particular, the funnel cover 3, the flow-through pipe 8 and the cross plate 11 are integrally connected.
The application method of the zooplankton sample washing device in the embodiment is as follows:
first, a bolting silk with a size corresponding to the size of the inner funnel 1 is selected, the bolting silk is wetted in advance, and is attached to the inner wall of the inner funnel 1, and the bolting silk is fixed by the fixing clamp 6 on the inner funnel 1. Through first buckle 9 and the combination of second buckle 10, cover funnel lid 3, flow into the bolting silk bottom with zooplankton sample through runner pipe 8 that funnel lid 3 is connected, reach the collecting region, the lateral wall hole 5 that the 1 lateral wall of inner funnel set up can accelerate filter speed, and the outer funnel 2 that connects through fixed horizontal bar 7 can collect the liquid that the 1 lateral wall of inner funnel spilled over.
Then the funnel cover 3 is opened, the circulating pipe 8 is removed, the porous separation plate 4 is placed on the collecting area, the circulating pipe 8 penetrates through the circulating opening 16, the porous separation plate 4 is fixed through the transverse plate 11 on the circulating pipe 8, and the funnel cover 3 is covered after the first buckle 9 and the second buckle 10 are combined.
The runner 8 external water pipe, rivers pass through runner 8 and get into the collecting region, and at the collecting region, rivers carry out continuous washing to the formaldehyde on sample surface, and later rivers constantly filter out the funnel through the bolting silk of porous separation plate 4 upper and lower part, and the zooplankton sample remains always in the collecting region after 4 interceptions of porous separation plate.
According to the inner funnel, the fixing clamp arranged at the top of the first conical cup body can fix the bolting silk, so that the bolting silk is prevented from being askew by flowing water, and a sample is prevented from being scattered and leaked; dense side wall holes are formed in the side wall of the filter, so that the filtering speed can be effectively increased; the funnel cover has the advantages that in the sample washing process, water flow splashing and surrounding environment pollution are avoided; the flow tube is connected with the sample collecting area, so that the sample can be conveniently and quickly drained to the sample collecting area, and the transverse plate arranged at the lower end of the flow tube has the function of fixing the porous separation plate; according to the porous separation plate, the separation holes in the porous separation plate only enable water molecules to freely pass through, so that a sample is always kept in the collection region, and the effect of separating water from the sample is achieved; the outer funnel has the functions of collecting liquid seeped out from the side wall hole of the inner funnel and fixing the funnel cover.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference signs in the claims are not intended to be construed as limiting the claim concerned.
The principle and the implementation mode of the invention are explained by applying a specific example, and the description of the embodiment is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.

Claims (10)

1. A zooplankton sample washing device comprises an inner funnel, an outer funnel and a funnel cover, wherein the inner funnel is installed in the outer funnel, and the funnel cover covers the top of the outer funnel; the method is characterized in that: a plurality of side wall holes are formed in the side wall of the cup body of the inner funnel, a filtering device is arranged on the inner wall of the cup body of the inner funnel, and a collecting area is arranged at the bottom of the filtering device; a circulating pipe is arranged on the funnel cover, and the bottom of the circulating pipe extends into the collecting area; and a porous separation plate is arranged above the collection area, a circulation port and a plurality of separation holes are formed in the porous separation plate, and the diameter of the circulation port is matched with that of the circulation pipe and used for enabling the circulation pipe to pass through.
2. The zooplankton sample washing device of claim 1, characterized in that: interior funnel includes first toper cup and the first cylindrical cup of an organic whole connection, the diameter of first toper cup reduces from top to bottom in proper order, the diameter of first cylindrical cup with the bottom diameter of first toper cup equals, the top of first cylindrical cup with the bottom of first toper cup is connected.
3. The zooplankton sample washing device of claim 2, characterized in that: the side wall of the first conical cup body is provided with a plurality of side wall holes, and the diameter of each side wall hole is larger than or equal to 2 cm.
4. A zooplankton sample washing device of claim 3, characterized in that: the filter device adopts a bolting silk which is attached to the inner wall of the first conical cup body; the top end of the first conical cup body is provided with a fixing clamp for fixing the bolting silk; the fixation clamp is provided with 3 along the top edge of first toper cup equidistant.
5. The zooplankton sample washing device of claim 2, characterized in that: the outer funnel comprises a second conical cup body and a second cylindrical cup body which are integrally connected, the diameter of the second conical cup body is sequentially reduced from top to bottom, the diameter of the second cylindrical cup body is equal to the diameter of the bottom of the second conical cup body, and the top of the second cylindrical cup body is connected with the bottom of the second conical cup body;
the top outer edge of the first conical cup body is connected with the top inner edge of the second conical cup body through a fixed transverse strip, and the number of the fixed transverse strip is 3 along the top outer edge of the first conical cup body at equal intervals.
6. The zooplankton sample washing device of claim 5, characterized in that: the outer edge of the top of the first conical cup body, the fixed transverse strip and the inner edge of the top of the second conical cup body are connected into a whole.
7. The zooplankton sample washing device of claim 5, characterized in that: the outer side of the top end edge of the second conical cup body is provided with 3 first buckles at equal intervals, the outer side of the cover edge of the funnel cover is provided with 3 second buckles at equal intervals correspondingly, and the funnel cover and the second conical cup body are connected through the first buckles and the second buckles.
8. The zooplankton sample washing device of claim 1, characterized in that: the lower end of the circulating pipe is connected with a transverse plate, and the transverse plate is used for fixing the porous separation plate.
9. The zooplankton sample washing device of claim 8, wherein: the funnel cover, the circulating pipe and the transverse plate are integrally connected.
10. The zooplankton sample washing device of claim 1, characterized in that: the aperture of the separation hole is smaller than 0.05mm, and the separation hole is used for intercepting zooplankton.
CN202110155769.0A 2021-02-04 2021-02-04 Zooplankton sample washing unit Active CN112945691B (en)

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CN117652487A (en) * 2023-12-05 2024-03-08 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) Device suitable for zooplankton keeps, washs and retrieves

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EP1734014A1 (en) * 2005-06-14 2006-12-20 PDL Development SL Device and method for filtration of fluids
CN203187390U (en) * 2012-12-22 2013-09-11 天津天雷科技有限公司 Controllable splash-proof funnel
US20170238464A1 (en) * 2014-11-24 2017-08-24 William R. Becker Apparatus and method for harvesting plankton and other biomass from a dead zone
CN205175734U (en) * 2015-11-19 2016-04-20 广州甘蔗糖业研究所 Sugarcane juice continuum appearance ware
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Publication number Priority date Publication date Assignee Title
CN117652487A (en) * 2023-12-05 2024-03-08 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) Device suitable for zooplankton keeps, washs and retrieves

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