CN102426124B - Device of pulling and flatting section for pathologic tissue frozen section and method thereof - Google Patents
Device of pulling and flatting section for pathologic tissue frozen section and method thereof Download PDFInfo
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- CN102426124B CN102426124B CN 201110349310 CN201110349310A CN102426124B CN 102426124 B CN102426124 B CN 102426124B CN 201110349310 CN201110349310 CN 201110349310 CN 201110349310 A CN201110349310 A CN 201110349310A CN 102426124 B CN102426124 B CN 102426124B
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Abstract
The invention discloses a device of pulling and flatting a section for a pathologic tissue frozen section and a method thereof. The device comprises a water bath box and a section pulling unit, wherein, one side of the water bath box near to the upper margin of the water bath box is provided with a liquid inlet, and one side of the water bath box near to the lower margin is provided with a liquid outlet; a recyclable or replaceable buffer solution is poured through the liquid inlet and liquid outlet, a support capable of being taken out slowly is hanged in the water bath box, and the level of the support is provided with a glass slide sinking to below the liquid level of the buffer solution; the pathologic tissue frozen section is placed in the buffer solution above the glass slide; the section pulling unit has a structure of scissors with handles, and the end portions of the two front forks are connected with metal wires. The method is characterized in that: when pulling the section, the two forks are put in the buffer solution, and the metal wires of the end portions of the two forks are allowed to hold the pathologic tissue frozen section; when flatting the section, the metal wires of the end portions of the two forks of the section pulling unit are separated by the handles of the scissors to flat the pathologic tissue frozen section; then the pathologic tissue frozen section is lightly put on the glass slide, the section pulling unit is pulled out, and finally, the support bearing the glass slide with the section is slowly taken out from the water bath box.
Description
Technical field
The present invention relates to a kind of be used in biology or medical experiment that the pathological tissue frozen section makes drag for sheet, exhibition sheet (paster) device and method.
Background technology
Frozen section is the method for widespread use the most in the conventional tabletting technology of histology.Frozen section not only is used for observing the morphosis of normal cell tissue, also be subjects such as clinical, scientific research pathology and medical jurisprudence in order to research, observe and judge the main method of the metamorphosis of cell tissue, and quite be widely used in the research of other many ambits.In scientific experiment, clinical medicine and experimental teaching, observe the section preparation great majority under the light microscopic and prepare by the frozen section method.The cell or tissue of living mostly is water white transparency, all lacks contrast between the various structures between various tissues and in the cell, is difficult for clear difference the out under general light microscopic; Tissue leaves behind the body will soon be dead and to produce tissue corrupt, loses original normal configuration, and therefore, tissue will be fixed through medium, frozen section, remove step such as medium and dyeing in order to avoid cell tissue death, and can its morphosis of clear identification.Frozen section manufacturing process comprise draw materials, fix, wash and dewater, add medium, frozen section, drag for sheet, steps such as exhibition sheet, paster, dyeing, dehydration, transparent, mounting.Yet, paraffin section generally is fixed to mounting and makes slide sample and need a few days from drawing materials, manufacturing process is longer, step is a lot, one step often caused accidentally that all that has been achieved is spoiled, and these processing cause and organize piece to shrink more or less, was subjected to humidity effect bigger during section, these weak points must be taken seriously in the film-making process, reduce its harmful effect as far as possible.But frozen section has exactly overcome long this shortcoming of time.
The flaking method of frozen section has two kinds, and a kind of method is to thinner histotomy, generally uses direct paster method.Another kind method is to thicker histotomy (being usually used in the immunohistochemical method of bleachinging and dyeing), opens up the sheet method with dragging for sheet in the making.Former approach is fairly simple, and latter's program more complicated is wanted constantly section to be reaped in the different liquid to go.The sheet method of dragging in the past all is that draw point is made crotch or dragged for sheet with the method for comparisons such as oese soil, passes through rotor for several times then, will cut into slices with little writing brush and slowly launch to pave.And so easy disorganize of sheet of dragging for, success ratio is quite low.The exhibition sheet occurs mashed sheet easily or overlapping phenomenon occurs.Namely enable to catch look, histotomy inside also can't effectively be observed at microscopically because of destructurized.Up to now, drag for the method for operating of avoiding the disorganize section that sheet and paster still do not have high-efficient simple, the experimenter is perplexed, directly influence the experiment process.
Summary of the invention
The present invention is directed to the existing problem of background technology, provide a kind of simply, easily and fast be used for the sheet exhibition of the dragging for sheet device and method that the pathological tissue frozen section is made, can significantly save time, reduce the mashed sheet of tissue in the experimentation and organize overlapping.
For reaching above purpose, the present invention takes following technical scheme to be achieved:
A kind of sheet exhibition of dragging for sheet device for the pathological tissue frozen section comprises a water-bath box, it is characterized in that, comprises that is also dragged for a sheet device; The one side of described water-bath box is provided with an inlet near the top, and the one side of water-bath box is provided with a liquid outlet near bottom; Be perfused with capable of circulation or removable damping fluid by inlet, liquid outlet in the water-bath box, hang one in the water-bath box and can slowly take out support, on the support level face microslide is set, microslide is submerged in buffering liquid level below; The pathological tissue frozen section places the damping fluid of microslide top; The described sheet device that drags for is band handle scissor construction, the angle that its two front forks end is connected with tinsel and shear shank less than the straight angle greater than the right angle.
In the such scheme, describedly drag for sheet device two front fork ends a diameter is respectively arranged is 4mm, length is the screw of 10mm, and there is the sulculus of a 1.5mm centre of screw, and described tinsel is set in this sulculus, by nut with tinsel firm be fixed on the fork end of body.The angle of tinsel and shear shank is 135 degree.
Realize that with aforementioned means the pathological tissue frozen section drags for the method for sheet exhibition sheet, it is characterized in that, comprise the steps,
(1) support is hung in the water-bath box, places the microslide of wash clean on the support level face;
(2) block liquid outlet, damping fluid is filled in the water-bath box by inlet; Make microslide be submerged in buffering liquid level below;
(3) the pathological tissue frozen section is placed the damping fluid of microslide top soak certain hour, finish the experimentation in liquid;
(4) drag for sheet exhibition sheet, when dragging for sheet, two front forks that drag for the sheet device are put in the damping fluid, and the tinsel that two front fork ends are connected is held the pathological tissue frozen section; During the exhibition sheet, the tinsel that will drag for sheet device two front fork ends by scissors handle separates, and the pathological tissue frozen section is launched;
(5) paster is placed on the pathological tissue frozen section that launches on the microslide gently, takes out and drags for the sheet device, and the support that will carry the microslide that has posted sheet at last slowly takes out from the water-bath box.
Compared with prior art, advantage of the present invention is, and is convenient and simple for operation; The exhibition sheet is quick, paster is smooth, can significantly save experimental period, reduces the mashed sheet of tissue in the experimentation and organizes overlapping.
Description of drawings
The present invention is described in further detail below in conjunction with the drawings and the specific embodiments.
Fig. 1 is the structural drawing of apparatus of the present invention water-bath box part.
Fig. 2 drags for the structural drawing of sheet device for apparatus of the present invention.
Among Fig. 1-Fig. 2,1, the water-bath box; 2, microslide; 3, pathological tissue frozen section; 4, inlet; 5, support; 6, damping fluid; 7, liquid outlet; 8, front fork; 9, front fork end; 10, tinsel.
Embodiment
The sheet exhibition of the dragging for sheet device that the present invention is used for the pathological tissue frozen section comprises a corrosion resistant sheet device that drags for; An exhibition film magazine and an exhibition horse.This three mutually combines, finally constitute one be used for that the pathological tissue frozen section makes drag for the sheet paster apparatus.
As shown in Figure 1, the exhibition film magazine is a water-bath box 1, is the rectangular parallelepiped box of a 80mm * 28mm * 25mm.There is an inlet 4 at 8mm place, top apart from the box one side, and one side is pasted the bottom relatively a liquid outlet 7.The exhibition horse is a support 5, puts a microslide 2 on the support level, because this support 5 is provided with four hooks, microslide 2 unsettled hanging in two face upper edge water-bath boxes can slowly can be taken out from water-bath box 1.By inlet 4, in the water-bath box, send phosphate buffer with the liquid feeding bottle, microslide is submerged in buffering liquid level below; Pathological tissue frozen section 3 places the damping fluid of microslide top, makes it become the exhibition sheet and drags for the sheet system, to reduce the flowability of tissue in liquid to greatest extent, adapts to the needs that the exhibition sheet drags for sheet.
As shown in Figure 2, drag for the sheet device and be band handle scissor construction, its two front forks end 9 is connected with tinsel 10 and drags for sheet device two front fork ends a diameter is respectively arranged is 4mm, length is the screw of 10mm, there is the sulculus of a 1.5mm centre of screw, tinsel 10 is set in this sulculus, by nut with tinsel firm be fixed on the fork end of body.The angle of tinsel and shear shank greater than the right angle, is 135 degree less than the straight angle in the present embodiment.
Realize that with aforementioned means the pathological tissue frozen section drags for the method for sheet exhibition sheet, comprise the steps,
(1) support 5 is hung in the water-bath box 1, places the microslide 2 of wash clean on the support level face;
(2) block liquid outlet 7, damping fluid 6 is filled in the water-bath box by inlet 4; Make microslide be submerged in buffering liquid level below;
(3) pathological tissue frozen section 3 is placed the damping fluid of microslide top soak certain hour (being no less than 10 minutes according to requirement of experiment), finish the experimentation in liquid;
(4) drag for sheet exhibition sheet, when dragging for sheet, two front forks 8 that drag for the sheet device are put in the damping fluid, and the tinsel 10 that two front fork ends are connected is held the pathological tissue frozen section; During the exhibition sheet, the tinsel that will drag for sheet device two front fork ends by scissors handle separates, and the pathological tissue frozen section is launched;
(5) paster is placed on the pathological tissue frozen section that launches on the microslide gently, takes out and drags for the sheet device, and the support that will carry the microslide that has posted sheet at last slowly takes out from the water-bath box.
After more than dragging for the end of sheet exhibition sheet, can open liquid outlet 7, damping fluid 6 be emitted replaceable different types of damping fluid.
In step (2), also can block liquid outlet 7, damping fluid 6 is filled in the water-bath box by inlet 4, and make damping fluid keep certain liquid height (being higher than microslide) by liquid outlet 7, in the water-bath box, circulate.
Claims (4)
1. the sheet exhibition of dragging for a sheet device that is used for the pathological tissue frozen section comprises a water-bath box, it is characterized in that, comprises that is also dragged for a sheet device; The one side of described water-bath box is provided with an inlet near the top, and the another side of water-bath box is provided with a liquid outlet near bottom; Be perfused with capable of circulation or removable damping fluid by inlet, liquid outlet in the water-bath box, hang one in the water-bath box and can slowly take out support, on the support level face microslide is set, microslide is submerged in buffering liquid level below; The pathological tissue frozen section places the damping fluid of microslide top; The described sheet device that drags for is band handle scissor construction, and its two front forks end is connected with tinsel, the angle of tinsel and shear shank less than the straight angle greater than the right angle.
2. the sheet that drags for for the pathological tissue frozen section as claimed in claim 1 is opened up the sheet device, it is characterized in that, describedly drag for sheet device two front fork ends a diameter is respectively arranged is 4mm, length is the screw of 10mm, there is the sulculus of a 1.5mm centre of screw, described tinsel is set in this sulculus, by nut with tinsel firm be fixed on the fork end of body.
3. the sheet exhibition of the dragging for sheet device for the pathological tissue frozen section as claimed in claim 1 is characterized in that the angle of tinsel and shear shank is 135 degree.
4. realize that the pathological tissue frozen section drags for the method for sheet exhibition sheet for one kind, it is characterized in that, based on the described sheet exhibition of the dragging for sheet device for the pathological tissue frozen section of claim 1, comprise the steps:
(1) support is hung in the water-bath box, places the microslide of wash clean on the support level face;
(2) block liquid outlet, damping fluid is filled in the water-bath box by inlet; Make microslide be submerged in buffering liquid level below;
(3) the pathological tissue frozen section is placed the damping fluid of microslide top soak certain hour, finish the experimentation in liquid;
(4) drag for sheet exhibition sheet, when dragging for sheet, two front forks that drag for the sheet device are put in the damping fluid, and the tinsel that two front fork ends are connected is held the pathological tissue frozen section that has melted; During the exhibition sheet, the tinsel that will drag for sheet device two front fork ends by scissors handle separates, and the pathological tissue frozen section that has melted is launched;
(5) paster is placed on the pathological tissue frozen section that melts that launches on the microslide gently, takes out and drags for the sheet device, and the support that will carry the microslide that has posted sheet at last slowly takes out from the water-bath box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110349310 CN102426124B (en) | 2011-11-08 | 2011-11-08 | Device of pulling and flatting section for pathologic tissue frozen section and method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110349310 CN102426124B (en) | 2011-11-08 | 2011-11-08 | Device of pulling and flatting section for pathologic tissue frozen section and method thereof |
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| CN102426124A CN102426124A (en) | 2012-04-25 |
| CN102426124B true CN102426124B (en) | 2013-07-03 |
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| CN 201110349310 Expired - Fee Related CN102426124B (en) | 2011-11-08 | 2011-11-08 | Device of pulling and flatting section for pathologic tissue frozen section and method thereof |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103822805A (en) * | 2012-11-16 | 2014-05-28 | 苏州药明康德新药开发有限公司 | Pathological section expanding method |
| CN103376238B (en) * | 2013-07-22 | 2016-01-06 | 华中科技大学 | A kind of metabolism information acquisition method and device |
| CN105500232A (en) * | 2016-01-19 | 2016-04-20 | 浙江中医药大学 | Fishing hook with adjustable darkroom light source |
| CN106053163B (en) * | 2016-04-28 | 2019-11-15 | 纪常生 | Organize film-making processing instrument |
| CN110018033B (en) * | 2019-05-16 | 2021-08-10 | 鹤壁市人民医院 | Pathological specimen preparation device |
| CN110618008B (en) * | 2019-10-24 | 2021-11-05 | 中南大学湘雅三医院 | Pathological section exhibition piece drags for piece integer machine |
| CN110823660A (en) * | 2019-11-26 | 2020-02-21 | 南通大学 | Tissue floating slice paving device and method for floating slice paving |
| CN110849696B (en) * | 2019-12-03 | 2022-03-11 | 中国科学院深圳先进技术研究院 | Biological tissue fixing and embedding device for three-dimensional imaging |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201229271Y (en) * | 2008-06-05 | 2009-04-29 | 张简庆宾 | Disposer for observation slice for pathological organization |
| CN201642180U (en) * | 2010-03-08 | 2010-11-24 | 张启瑜 | Artery sending forceps |
| WO2011046807A2 (en) * | 2009-10-12 | 2011-04-21 | Ventana Medical Systems, Inc. | Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201229271Y (en) * | 2008-06-05 | 2009-04-29 | 张简庆宾 | Disposer for observation slice for pathological organization |
| WO2011046807A2 (en) * | 2009-10-12 | 2011-04-21 | Ventana Medical Systems, Inc. | Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue |
| CN201642180U (en) * | 2010-03-08 | 2010-11-24 | 张启瑜 | Artery sending forceps |
Non-Patent Citations (2)
| Title |
|---|
| 王华等.病理组织切片中常见问题及解决方法.《实验科学与技术》.2010,第8卷(第5期),全文. |
| 病理组织切片中常见问题及解决方法;王华等;《实验科学与技术》;20101031;第8卷(第5期);全文 * |
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| CN102426124A (en) | 2012-04-25 |
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