CN112557159B - Specimen embedding device and frozen slicing method - Google Patents
Specimen embedding device and frozen slicing method Download PDFInfo
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- CN112557159B CN112557159B CN202011349614.2A CN202011349614A CN112557159B CN 112557159 B CN112557159 B CN 112557159B CN 202011349614 A CN202011349614 A CN 202011349614A CN 112557159 B CN112557159 B CN 112557159B
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000007493 shaping process Methods 0.000 claims abstract description 9
- 230000008014 freezing Effects 0.000 claims abstract description 7
- 238000007710 freezing Methods 0.000 claims abstract description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 23
- 238000003780 insertion Methods 0.000 claims description 4
- 230000037431 insertion Effects 0.000 claims description 4
- 229930040373 Paraformaldehyde Natural products 0.000 claims description 3
- 230000018044 dehydration Effects 0.000 claims description 3
- 238000006297 dehydration reaction Methods 0.000 claims description 3
- 229920002866 paraformaldehyde Polymers 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a specimen embedding device and a frozen slicing method, which comprise a specimen flitch, a specimen box, a sleeve and a positioning seat, wherein a supporting rod is fixedly arranged at the middle end of the bottom side of the specimen flitch, a box body is movably arranged on the supporting rod through a bearing, the specimen flitch is positioned in the box body, an inner ring of the box body is provided with an inner thread a, and the bottom side of the box body is provided with a slot. According to the invention, through the arranged specimen flitch, the specimen box, the sleeve and the positioning seat, when the tissue embedding machine is used, the proper shaping layer is selected, the shaping shape of the embedding agent is customized, so that the excised specimen is more attractive, then after the tissue is placed into the embedding agent for freezing, the position of the slice can be quickly changed directly through the screw thread structure between the specimen box, the box body and the sleeve, and the slice is attached to the specimen flitch, and in the process, the embedding agent for bonding the slice and the specimen flitch cannot leak from the specimen flitch, so that the trouble of cleaning the specimen flitch in the later period of staff is avoided.
Description
Technical Field
The invention relates to the technical field of biomedicine, in particular to a specimen embedding device and a frozen section method.
Background
With the progressive completion of genome sequencing in humans and other species, research in modern biology has entered the post-genomic era, the functional genomic era. In recent years, with the development of immunohistochemical experiments, frozen sections are widely used in various fields of biology, and a clinician can perform pathological examination on the diagnosis of a surgical scheme through the frozen sections in the surgical process, wherein the frozen sections play a very important role in the process, and embedding agents are key to the quality of the sections. The prior art embedding method mainly comprises the steps of placing a tissue sample into embedding liquid, solidifying the embedding liquid by some means, sticking the solidified block of the embedding liquid on a sticking board, and slicing. At present, in the preparation in-process of frozen section, not only frozen section sample embedding is inhomogeneous, and the shape is single, and after the embedding liquid solidification, still need to shift it to the sample board, in this in-process, before frozen section pastes the sample board, still need add the embedding agent on the sample board, guarantee the stability of section, this process not only can't realize the quick transfer of embedding piece, and the embedding agent that adds also flows easily under the sample board on the sample board, causes the inconvenience of later stage sample board clearance.
Disclosure of Invention
The invention aims to: the invention aims at solving the problems and achieving the aims by improving and innovating the defects in the background technology or the prior art, and aims at providing a sample embedding device and a frozen section method, which are realized by the following design structures and technical schemes:
the utility model provides a sample embedding device, includes sample flitch, specimen-box, sleeve pipe and positioning seat, the bottom side middle-end fixed mounting of sample flitch has branch, branch is provided with the box body through the bearing activity, the sample flitch is located the box body, the inner circle of box body is provided with internal thread a, the slot has been seted up to the bottom side of box body; an inner ring of the specimen box is provided with an inner thread b, and a rubber ring is adhered to the bottom side of the interior of the specimen box; the left side and the right side of the sleeve are fixedly provided with inserting blocks a, the outward sides of the two inserting blocks a in the horizontal direction are provided with inserting holes, the lower side of the outer ring of the sleeve is provided with external threads, and the inner ring of the sleeve is provided with a shaping layer; the front surface of the positioning seat is provided with a groove, the middle end of the rear side inside the groove is fixedly provided with a rotating motor, a shaft head of a motor shaft of the rotating motor is fixedly provided with an inserting block b, the left side and the right side of the groove are respectively provided with a positioning groove, the left side and the right side inside the positioning seat are respectively fixedly provided with a push rod motor, and motor shafts of the two push rod motors are respectively movably penetrated through the corresponding positioning grooves; the socket is matched with the plug block b, the plug block a is matched with the positioning groove, the outer diameter of a motor shaft of the push rod motor is equal to the inner diameter of the jack, the internal threads a and b are matched with the external threads, and the shortest distance between the outer diameter and the inner diameter of the rubber ring is equal to the shortest distance between the outer diameter and the inner diameter of the bottom of the sleeve.
As a preferred embodiment of the present invention, the shaping layer is used to customize the shape of the specimen slice.
A frozen section method adopts a specimen embedding device, which comprises the following steps:
a. preparing processed tissues, embedding agents, specimen pasting boards, specimen boxes, sleeves, freezers and slicing machines, wherein positioning seats are arranged at positions corresponding to blades in the slicing machines;
b. c, after the step a is completed, firstly, screwing the specimen box and the sleeve, injecting the embedding medium into the specimen box, then soaking the specimen box in 4-6% paraformaldehyde solution for more than 24 hours, and vertically inserting the tissues subjected to conventional dehydration into the embedding medium;
c. c, after the step b is completed, putting the specimen box and the sleeve containing the tissues and the embedding agent into a refrigerator to be frozen at the temperature of 0 ℃, and taking out the specimen box and the sleeve after the freezing is completed;
d. after the step c is completed, the specimen box is firstly separated from the sleeve in a rotating way, the specimen box is replaced by a box body which is connected with the sleeve in a rotating way, before that, embedding agent is added at the top of the specimen pasting board, and the frozen specimen is pasted on the specimen pasting board through the embedding agent;
e. after the step d is completed, the box body is inserted into the groove, the inserting block b is inserted into the slot, the two inserting blocks a are inserted into the positioning slots, and the motor shafts of the two push rod motor drives penetrate into the insertion holes to fix the inserting blocks a;
f. after step e is completed, the box body is rotated by starting the rotating motor, and the embedded specimen is driven to move outwards by the specimen pasting plate so as to facilitate slicing work of the slicing machine, and meanwhile, slicing is completed.
The invention has the beneficial effects that: the biological specimen manufactured by the invention has the advantage of customizing the shape of the specimen with high degree of freedom, solves the problem of single shape of the biological specimen manufactured by the prior art, greatly improves the embedding quality and the use value of the biological specimen, can realize the rapid transfer of the slice without removing the embedding block in the process of replacing the slice, directly replaces the box body with the specimen box, directly omits the step of taking out the embedding block and transferring the embedding block onto a specimen plate after the embedding liquid is solidified in the prior art, effectively improves the embedding efficiency, and does not cause the phenomenon that the embedding agent leaks from the specimen flitch in the prior art, thereby effectively improving the embedding quality.
Drawings
FIG. 1 is a front view of a specimen panel of a specimen embedding device of the present invention;
FIG. 2 is a front view of a cartridge of the present invention;
FIG. 3 is a top view of a cartridge of a sample embedding device of the present invention;
FIG. 4 is a perspective view of a sleeve of a specimen embedding device according to the present invention;
FIG. 5 is a front view of a positioning seat of a specimen embedding device according to the present invention;
FIG. 6 is a flow chart of a method of frozen section according to the present invention.
In the figure: 1. a specimen pasting board; 2. a specimen box; 3. a sleeve; 4. a positioning seat; 5. a support rod; 6. a bearing; 7. a case body; 8. an internal thread a; 9. a slot; 10. an internal thread b; 11. a rubber ring; 12. a plug a; 13. a jack; 14. an external thread; 15. shaping layer; 16. a groove; 17. a rotating motor; 18. a plug b; 19. a positioning groove; 20. a push rod motor.
Detailed Description
The invention will be further described with reference to the accompanying drawings, which are not intended to limit the invention to the embodiments described.
Referring to fig. 1-5, the present invention provides a technical solution: the utility model provides a sample embedding device, includes sample flitch 1, sample box 2, sleeve pipe 3 and positioning seat 4, the bottom side middle-end fixed mounting of sample flitch 1 has branch 5, branch 5 is provided with box body 7 through bearing 6 activity, sample flitch 1 is located box body 7, the inner circle of box body 7 is provided with internal thread a8, slot 9 has been seted up to the bottom side of box body 7; the inner ring of the specimen box 2 is provided with an internal thread b10, and the bottom side of the interior of the specimen box 2 is adhered with a rubber ring 11; the left side and the right side of the sleeve 3 are fixedly provided with inserting blocks a12, the outward sides of the two inserting blocks a12 in the horizontal direction are provided with inserting holes 13, the lower side of the outer ring of the sleeve 3 is provided with external threads 14, and the inner ring of the sleeve 3 is provided with a shaping layer 15; the front surface of the positioning seat 4 is provided with a groove 16, the middle end of the rear side inside the groove 16 is fixedly provided with a rotating motor 17, a shaft head of a motor shaft of the rotating motor 17 is fixedly provided with an inserting block b18, the left side and the right side of the groove 16 are provided with positioning grooves 19, the left side and the right side inside the positioning seat 4 are fixedly provided with push rod motors 20, and motor shafts of the two push rod motors 20 movably penetrate through the corresponding positioning grooves 19; the socket 9 is matched with the plug b18, the plug a12 is matched with the positioning groove 19, the outer diameter of a motor shaft of the push rod motor 20 is equal to the inner diameter of the insertion hole 13, the position of the plug a12 is conveniently limited by the expansion and contraction of the motor shaft of the push rod motor 20, the internal threads a8 and the internal threads b10 are matched with the external threads 14, the rotation connection between the specimen box 2, the box body 7 and the sleeve 3 is convenient, the shortest distance between the outer diameter and the inner diameter of the rubber ring 11 is equal to the shortest distance between the outer diameter and the inner diameter of the bottom of the sleeve 3, and the sealing when the specimen box 2 is connected with the sleeve 3 can be conveniently realized through the rubber ring 11.
The shaping layer 15 is used to customize the shape of the specimen slice.
Referring to fig. 6, the present invention further provides a technical solution: a frozen section method adopts a specimen embedding device which comprises the following steps:
a. ready for processing tissue, embedding medium, specimen taping 1, specimen cartridge 2, cannula 3, freezer and microtome, wherein positioning seat 4 is mounted in the microtome in a position corresponding to the blade;
b. after the step a is completed, the specimen box 2 and the sleeve 3 are screwed, the embedding medium is injected into the specimen box 2, then the tissue is soaked in 4-6% paraformaldehyde solution for more than 24 hours, and the tissue after conventional dehydration is vertically inserted into the embedding medium;
c. c, after the step b is completed, putting the specimen box 2 and the sleeve 3 containing the tissues and the embedding agent into a refrigerator to be frozen at the temperature of 0 ℃, and taking out the specimen box and the sleeve after the freezing is completed;
d. after the step c is completed, firstly, the specimen box 2 is rotationally separated from the sleeve 3, the specimen box 2 is replaced by a box body 7 which is rotationally connected with the sleeve 3, and before that, embedding agent is added at the top of the specimen pasting board 1, and the frozen specimen is pasted on the specimen pasting board 1 through the embedding agent;
e. after the step d is completed, the box body 7 is inserted into the groove 16, the inserting block b18 is inserted into the slot 9, the two inserting blocks a12 are inserted into the positioning grooves 19, and the motor shafts are driven by the two push rod motors 20 to penetrate into the insertion holes 13, so that the inserting blocks a12 are fixed;
f. after step e is completed, the box body 7 is rotated by starting the rotating motor 17, and the embedded specimen is driven to move outwards by the specimen pasting board 1 so as to facilitate the slicing work of the slicing machine, and meanwhile, slicing is completed.
When the invention is used, firstly, the processed tissues, embedding agent, a sample flitch 1, a sample box 2, a sleeve 3, a refrigerator and a slicer are prepared, wherein a positioning seat 4 is arranged at a position corresponding to a blade in the slicer, then the sample box 2 and the sleeve 3 are screwed, the embedding agent is injected into the sample box 2, then the tissues are vertically inserted into the embedding agent, then the sample box 2 and the sleeve 3 containing the tissues and the embedding agent are placed into the refrigerator for freezing, after the freezing is finished, the tissues are taken out, then the sample box 2 and the sleeve 3 are rotationally separated, the sample box 2 is replaced by a box body 7 and the sleeve 3, the embedding agent is added at the top of the sample flitch 1 before the freezing, the sample is stuck on the sample flitch 1 through the embedding agent, then the box body 7 is inserted into a groove 16, a plug block b18 is inserted into a slot 9, two plug blocks a12 are inserted into a positioning groove 19, a motor shaft 13 is driven by two push rod motors 20 to penetrate into a plug block 13, finally, the box body 7 is replaced by the box body 7 and the cut into the cut slice is started, and finally, the cut slice is conveniently and the slice is moved outwards through the slicing machine.
The foregoing describes in detail preferred embodiments of the present invention. It should be understood that numerous modifications and variations can be made in accordance with the concepts of the invention by one of ordinary skill in the art without undue burden. Therefore, all technical solutions which can be obtained by logic analysis, reasoning or limited experiments based on the prior art by the person skilled in the art according to the inventive concept shall be within the scope of protection defined by the claims.
Claims (3)
1. The utility model provides a sample embedding device, includes sample flitch (1), specimen-box (2), sleeve pipe (3) and positioning seat (4), its characterized in that: a supporting rod (5) is fixedly arranged at the middle end of the bottom side of the specimen pasting board (1), a box body (7) is movably arranged on the supporting rod (5) through a bearing (6), the specimen pasting board (1) is positioned in the box body (7), an inner ring of the box body (7) is provided with an inner thread a (8), and a slot (9) is formed in the bottom side of the box body (7);
an inner ring of the specimen box (2) is provided with an internal thread b (10), and a rubber ring (11) is adhered to the bottom side of the interior of the specimen box (2);
the two sides of the sleeve (3) are fixedly provided with inserting blocks a (12), inserting holes (13) are formed in the outward side of the two inserting blocks a (12) in the horizontal direction, external threads (14) are formed in the lower side of the outer ring of the sleeve (3), and a shaping layer (15) is arranged in the inner ring of the sleeve (3);
the front of the positioning seat (4) is provided with a groove (16), the middle end of the rear side inside the groove (16) is fixedly provided with a rotating motor (17), a shaft head of a motor shaft of the rotating motor (17) is fixedly provided with an inserting block b (18), the left side and the right side of the groove (16) are provided with positioning grooves (19), the left side and the right side inside the positioning seat (4) are fixedly provided with push rod motors (20), and motor shafts of the two push rod motors (20) are movably penetrated through the corresponding positioning grooves (19);
the plug-in socket is characterized in that the slot (9) is matched with the plug-in block b (18), the plug-in block a (12) is matched with the positioning groove (19), the outer diameter of a motor shaft of the push rod motor (20) is equal to the inner diameter of the insertion hole (13), the internal threads a (8) and the internal threads b (10) are matched with the external threads (14), and the shortest distance between the outer diameter and the inner diameter of the rubber ring (11) is equal to the shortest distance between the outer diameter and the inner diameter of the bottom of the sleeve (3).
2. The specimen embedding device of claim 1, wherein: the shaping layer (15) is used for customizing the shape of the specimen slice.
3. A method of frozen section using a specimen embedding apparatus as claimed in any one of claims 1-2, characterized in that: the method comprises the following steps:
a. preparing processed tissues, embedding agents, a specimen pasting board (1), a specimen box (2), a sleeve (3), a refrigerator and a slicing machine, wherein a positioning seat (4) is arranged at a position corresponding to a blade in the slicing machine;
b. after the step a is completed, the specimen box (2) is screwed with the sleeve (3), the embedding agent is injected into the specimen box (2), then the specimen box is soaked in 4-6% paraformaldehyde solution for more than 24 hours, and the tissue after conventional dehydration is vertically inserted into the embedding agent;
c. c, after the step b is completed, putting the specimen box (2) containing the tissues and the embedding agent and the sleeve (3) into a refrigerator to be frozen at the temperature of 0 ℃, and taking out the specimen box after the freezing is completed;
d. after the step c is completed, firstly, the specimen box (2) is rotationally separated from the sleeve (3), the specimen box (2) is replaced by a box body (7) which is rotationally connected with the sleeve (3), before that, embedding agent is added at the top of the specimen pasting board (1), and the frozen specimen is pasted on the specimen pasting board (1) through the embedding agent;
e. after the step d is completed, the box body (7) is inserted into the groove (16), the inserting block b (18) is inserted into the slot (9), the two inserting blocks a (12) are inserted into the positioning groove (19), and the motor shafts are driven by the two push rod motors (20) to penetrate into the inserting holes (13), so that the inserting blocks a (12) are fixed;
f. after the step e is finished, the rotating motor (17) is started to rotate the box body (7), and the embedded specimen is driven to move outwards through the specimen pasting board (1) so as to facilitate the slicing work of the slicing machine, and meanwhile, the slicing is finished.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011349614.2A CN112557159B (en) | 2020-11-26 | 2020-11-26 | Specimen embedding device and frozen slicing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011349614.2A CN112557159B (en) | 2020-11-26 | 2020-11-26 | Specimen embedding device and frozen slicing method |
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| Publication Number | Publication Date |
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| CN112557159A CN112557159A (en) | 2021-03-26 |
| CN112557159B true CN112557159B (en) | 2023-11-07 |
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| CN202011349614.2A Active CN112557159B (en) | 2020-11-26 | 2020-11-26 | Specimen embedding device and frozen slicing method |
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|---|---|---|---|---|
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| GB8609312D0 (en) * | 1986-04-16 | 1986-05-21 | Pa Consulting Services | Preparing tissue specimens |
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| CL2017003244A1 (en) * | 2015-06-25 | 2018-04-20 | Immatics Biotechnologies Gmbh | New cell epitopes and new combinations of cell epitopes for use in the immunotherapy of myeloma and other types of cancer |
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2020
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