CN203908836U - Automatic device for preparing cell wax blocks - Google Patents
Automatic device for preparing cell wax blocks Download PDFInfo
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- CN203908836U CN203908836U CN201420200440.7U CN201420200440U CN203908836U CN 203908836 U CN203908836 U CN 203908836U CN 201420200440 U CN201420200440 U CN 201420200440U CN 203908836 U CN203908836 U CN 203908836U
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- test tube
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- cell block
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Abstract
The utility model provides an automatic device for preparing cell wax blocks. The automatic device comprises a rotary table, a test tube, a semi-permeable membrane, a liquid taking device, an extraction device and a pressurizing device, wherein the test tube is fixed in a first fixing hole in the rotary table; the test tube comprises a first tube body and a second tube body which are in dissociable connection with each other; an opening is formed in the bottom of the second tube body; the semi-permeable membrane is arranged between the first tube body and the second tube body; the liquid taking device used for sucking cell sap, reagents and liquid paraffin comprises a mechanical arm, a straw, a first guide pipe and a work pump; the extraction device comprises a fixing part and a second guide pipe; the fixing part adopts a hollow structure; an opening is formed in the top of the fixing part and is fixedly connected with an opening in the bottom of the second tube body; an opening is formed in the bottom of the fixing part and is fixedly connected with one end of the second tube body; the pressurizing device is connected with the other end of the second guide pipe, and provides positive pressure or negative pressure for the bottom of the test tube through the second guide pipe.
Description
Technical field
The utility model relates to medical electronics instrument field, and is particularly related to a kind of device of automatically preparing cell block.
Background technology
Medically processing body fluid cell is generally that the cell with liquid is directly coated on glass sheet, then observes by microscope.Along with cytopathologic development in recent years, the limitation of the common smear that above this routine techniques is made is more and more obvious, as there is uniqueness in smear, and reproducible not; Can not forever retain; Hindered the application that emerging pathological technique learns a skill as SABC, molecular pathology.
Cell block is to fix carrying out paraffin after the cell extraction in liquid, makes the form of allied organization's section after fixing.The method has solved the problem that above-mentioned common smear exists, and also can avoid the doctor-patient dispute that may cause because of smear damage, loss etc.Make cell block is also the basis of preparing organization chip simultaneously, and this technology will be widely used in cell pathology field.
Making at present cell block is to adopt manual method to process substantially, and the body fluid cell of processing can comprise hydrothorax, ascites, cervical exfoliated cell, dropsy of serous cavity, cerebrospinal fluid, sputum etc.Make concise and to the point step by hand as follows: pour the body fluid of extraction into test tube, by the test tube high speed centrifugation some time, remove the supernatant that test tube top has comprised the interference components such as blood, make the liquid cell of collecting form cell mass; Carefully peel off lower complete cell mass, with permeable filter paper, wrap, go through the series of steps such as fixing, dehydration, transparent, dyeing, waxdip, cooling, embedding, then by embedded wax stone in microtome incision the thin slice into about 5 μ m, thereby finally complete cell block.
In above step, dehydration, transparent, waxdip can automatically complete in tissue processor, but all the other steps are all to complete by craft now, consuming timely be about 7～8 hours, after certain step completes during this time, operating personnel must manual intervention just can carry out next step, time-consuming, effort.And make cell block by hand, and operating personnel's experience level is directly related, otherwise probably there is the uneven situation of wax stone quality; Manual operations also may contact with the reagent such as formalin, dimethylbenzene in addition, and careless slightly operating personnel's the health of will giving be brought injury.
Utility model content
The utility model is made and is needed to adopt manual completing in order to overcome existing cell block, and not only performance accuracy is low and bring the problem of injury very easily to operator's health, and a kind of device of automatically preparing cell block is provided.
To achieve these goals, the utility model provides a kind of device of automatically preparing cell block, comprises rotating disk, test tube, semi-permeable diaphragm, liquid taking device, draw-out device and pressue device.Rotating disk has a plurality of the first fixed orifices.The quantity of test tube is at least one, and is fixed on the first fixed orifice.Test tube comprises the first body and the second body, and the first body and the second body are detachable connection, and the bottom of the second body has perforate.Semi-permeable diaphragm is arranged between the first body and the second body.Liquid taking device comprises mechanical arm, suction pipe, the first conduit and working barrel.Wherein mechanical arm is that test tube adds cell liquid, reagent and whiteruss, comprises bottom, rotation section and clamping part, and bottom has a plurality of through holes, and mechanical arm is hollow structure, and in rotation section, is provided with controller.Suction pipe is arranged at clamping part.The first conduit via through holes and mechanical arm are inner to be connected with suction pipe, for the circulation of liquid.Working barrel is arranged at the first conduit, and the reagent that is positioned at the reagent bottle of suction pipe below is drawn into suction pipe, and a first conduit correspondence is provided with a working barrel, working barrel and controller electric connection.
Draw-out device comprises fixture and the second conduit, and fixture is hollow structure, and the perforate of the open top of fixture and the second body bottom is fixedly connected, and one end of the bottom opening of fixture and the second conduit is fixedly connected.Pressue device is connected with the other end of the second conduit, by the second conduit, bottom test tube, provides malleation or negative pressure.
In the utility model one embodiment, the upper surface of semi-permeable diaphragm and lower surface are all coated with wire netting, and wire netting and semi-permeable diaphragm are all arranged between the first body and the second body.
In the utility model one embodiment, on the sidepiece of test tube, be provided with sensor, the controller in the electrical connection of rotating of sensor portion, the liquid level of liquid in the real-time test tubes of sensor.
In the utility model one embodiment, on the first conduit, be provided with solenoid valve, and a first conduit correspondence is provided with a solenoid valve, solenoid valve and controller electric connection.
In the utility model one embodiment, the second body is the inverted round stage shape of hollow, and the area that is positioned at the perforate of the second body bottom is less than the area of the second body bottom.
In the utility model one embodiment, the top of fixture is provided with the groove corresponding with the second body of hollow inverted round stage shape, and the both sides of groove are provided with working arm.
In the utility model one embodiment, the lateral wall of the first body is provided with stop block, and the joint face of the lower limb of stop block and the first body and the second body is in the same plane.
In the utility model one embodiment, the sidewall of groove is provided with bolster.
In sum, the device of automatically preparing cell block that the utility model provides, the first conduit in liquid taking device and working barrel add cell liquid to be prepared and the reagent that is arranged in reagent bottle to test tube by mechanical arm and suction pipe.Machinery is got liquid and has been replaced traditional liquid of manually getting, and reagent does not contact with operator, and operator's health can not come to harm.By controlling working barrel, also can reach the addition of controlling reagent, to compare precision higher with manual operation.By arrange pressue device soak formalin, dehydration, dyeing, transparent and soak the steps such as paraffin in constantly repeat to increase malleation or negative pressure to test tube bottom, make to be positioned at cell on semi-permeable diaphragm fully with the reagent reacting adding.User only need set the time that pressue device adds malleation and negative pressure on demand, can automatically complete said process.
In addition, upper surface and lower surface at semi-permeable diaphragm all arrange wire netting, when heating arrangement is during to paraffin pipeline and test tube, wire netting has good heat conduction function and the heat on test tube can be delivered on semi-permeable diaphragm, makes the paraffin on semi-permeable diaphragm in soaking paraffin process, keep liquid.
For above-mentioned and other objects, features and advantages of the present utility model can be become apparent, preferred embodiment cited below particularly, and coordinate accompanying drawing, be described in detail below.
Accompanying drawing explanation
Figure 1 shows that the device schematic diagram of automatically preparing cell block that the utility model one embodiment provides;
Figure 2 shows that the schematic cross-section of test tube in Fig. 1, fixture and pressue device;
Embodiment
Figure 1 shows that the device schematic diagram of automatically preparing cell block that the utility model one embodiment provides; Figure 2 shows that the schematic cross-section of test tube in Fig. 1, fixture and pressue device.See also Fig. 1 to Fig. 2.
The utility model provides a kind of device of automatically preparing cell block, comprises rotating disk 1, test tube 2, semi-permeable diaphragm 3, liquid taking device 4, draw-out device 5 and pressue device 6.Rotating disk 1 has a plurality of the first fixed orifices 11.The quantity of test tube 2 is at least one, and is fixed on the first fixed orifice 11.Test tube 2 comprises that the first body 21 and the second body 22, the first bodys 21 and the second body 22 are detachable connection, and the bottom of the second body 22 has perforate 221.Semi-permeable diaphragm 3 is arranged at 22 of the first body 21 and the second bodys.Liquid taking device 4 comprises mechanical arm 41, suction pipe 42, the first conduit 43 and working barrel 44.Wherein mechanical arm 41 is that test tube 2 adds reagent or whiteruss.Mechanical arm 41 comprises bottom 411, rotation section 412 and clamping part 413, and bottom 411 has a plurality of through holes.Mechanical arm 41 is hollow structure, and in rotation section 412, is provided with controller 8.Suction pipe 42 is arranged at clamping part 413.The first conduit 43 via through holes and mechanical arm 41 inside are connected with suction pipe 42, for the circulation of liquid.Working barrel 44 is arranged at the first conduit 43, and the reagent that is positioned at the reagent bottle of suction pipe 42 belows is drawn into suction pipe 42, and first conduit 43 correspondences are provided with a working barrel 44.Draw-out device 5 comprises fixture 51 and the second conduit 52, and fixture 51 is hollow structure, and the perforate 221 of the open top of fixture 51 and the second body 22 bottoms is fixedly connected, and one end of the bottom opening of fixture 51 and the second conduit 52 is fixedly connected.Pressue device 6 is connected with the other end of the second conduit 52, by the second conduit 52, provides malleation or negative pressure for test tube 2 bottoms.Wherein, on the first conduit 43, be also provided with solenoid valve 45, and first conduit 43 correspondences are provided with a solenoid valve 45.
In the present embodiment, liquid taking device 4 has two covers, wherein a set of for extracting the liquid such as cell liquid to be produced and formalin, alcohol and dimethylbenzene, and in this cover liquid taking device, the bottom 411 of mechanical arm 41 has four through holes, there are respectively four the first conduits 43 to be connected with the suction pipe 42 on this liquid taking device 4 through this corresponding through hole, be respectively used to draw formalin, concentration is 95% alcohol, anhydrous alcohol and dimethylbenzene, another set of liquid taking device 4 is for extracting paraffin.Yet the utility model is not limited in any way this.In other embodiment, can adopt a set of liquid taking device 4, but in the bottom of mechanical arm 41, more than five through holes is set, and two clamping parts 413 and two suction pipes 42 are set, one of them suction pipe 42 is for drawing the reagent such as formalin and cell liquid to be prepared, and another suction pipe 42 is for drawing paraffin.
The course of work of the device of automatically preparing cell block the utility model being provided below in conjunction with Fig. 1 and Fig. 2 describes in detail.
First, the cell liquid of hope being produced to cell block draws in the test tube 2 with semi-permeable diaphragm 3.Drawn and rear suction pipe has been rinsed.
Soak formalin.User opens working barrel 44 and the solenoid valve 45 on the first conduit 43 being connected with the reagent bottle that holds formalin, and suction pipe 42 is drawn the neutral formalin that 5ml concentration is 10% and is extremely placed with in the test tube 2 of cell liquid.
Open pressue device 6, by the second conduit 52, in test tube bottom, increase negative pressure, make moisture, little molecule and formalin liquid by semi-permeable diaphragm 3, the cell in cell liquid is trapped on semi-permeable diaphragm 3.On semi-permeable diaphragm 3, the cell of accumulation is more while blocking semi-permeable diaphragm 3.Pressue device 6 is set, and reversion control pump, increases malleation in test tube bottom, and the cell getting lodged on semi-permeable diaphragm 3 is pushed back in cell liquid again.Again increase again afterwards negative pressure, the liquid of mix reagent is sucked in waste liquid bottle by semi-permeable diaphragm 3.Constantly repeat said process, make all cells on semi-permeable diaphragm 3 all fully soak formalin, this process is lasted 2 hours, in this overshoot, must guarantee in vitro to have at least formalin liquid more than 2ml.
In order to guarantee in vitro to have at least formalin liquid more than 2ml, in the present embodiment, by the sidewall at test tube 2, sensor being set, this sensor and controller are electrically connected.Liquid level in the real-time test tubes 2 of controller, the liquid level in test tube 2 is during lower than 2ml, and controller output control signal is opened working barrel 44 and solenoid valve 45, and suction pipe 42 is drawn formalin in vitro, realizes the process of automatic soaking formalin.
Dehydration, this dehydration comprises primary dewatering and second dehydration, and the process of primary dewatering and second dehydration is identical with the process of soaking formalin, and just in primary dewatering, reagent used is that concentration is 95% alcohol, and dewatering time is 1.5 hours.And second dehydration reagent used is anhydrous alcohol, and dewatering time is also 1.5 hours.
Dyeing, dyes to the cell of second dehydration in interior interpolations of test tube 2 0.8ml Yihong, and constantly repeating increases negative pressure, malleation and negative pressure to test tube bottom, and the cell of delay on semi-permeable diaphragm 3 is fully dyeed, and this process is lasted 10 minutes.
Transparent, add dimethylbenzene in vitro the cell after dyeing is carried out to transparent processing, constantly repeating increases negative pressure, malleation and negative pressure to test tube 2 bottoms, and this process is lasted 30 minutes.
Soak paraffin, heating fills reagent bottle, the paraffin of paraffin and draws pipeline and test tube, paraffin in reagent bottle is melted, paraffin after melting is added in test tube 2, negative pressure, malleation and negative pressure are constantly repeated to increase in test tube 2 bottoms, this process is lasted 1.5 hours, and dimethylbenzene residual on cell after transparent processing is cemented out, and in this process, the temperature of test tube 2 is at 60 ℃～70 ℃.In the present embodiment, for preventing the paraffin cooled and solidified on semi-permeable diaphragm 3 in step S106, upper surface and lower surface at semi-permeable diaphragm 3 are all coated with wire netting 7, and wire netting 7 and semi-permeable diaphragm 3 are all arranged between the first body and the second body, wire netting 7 can pass to semi-permeable diaphragm 3 by the temperature of test tube 2 fast, makes the temperature of semi-permeable diaphragm 3 maintain 60 ℃～70 ℃.In addition, wire netting 5 also can be played a supporting role to semi-permeable diaphragm 2.
In above-mentioned steps, by pressue device 6, make to be trapped in reagent and the paraffin reaction repeated that cell on semi-permeable diaphragm 3 and each step are added, all processes are all automatically to complete, and user only need arrange the time of each step and the time that pressue device 6 adds malleation or negative pressure.Operator, without any reagent of contact, can not endanger the healthy of operator.Further, by increasing solenoid valve 45, by controlling the valve opening of solenoid valve 45, can realize the accurate absorption of reagent.And increase sensor, carry out the liquid level in test tubes 2, and the signal detecting is passed to controller, controller is controlled the duty of solenoid valve 45 and working barrel 44 according to this detection signal, realize automatically and controlling.
When step S106 completes and test tube 2 is removed cell block on semi-permeable diaphragm 3 to put into embedding frame and carry out embedding and embedded cell block is placed under microtome and is cut into slices after cooling.
In the present embodiment, the second body 22 is the inverted round stage shape of hollow, and the area that is positioned at the perforate 221 of the second body 22 bottoms is less than the area of the second body 22 bottoms.The top of the fixture 51 corresponding with this inverted round stage shape structure is provided with groove 511, and the both sides of groove 511 are provided with working arm 512.The second body 22 of inverted round stage shape structure can be concentrated liquid in the fixture 51 that flows into hollow.Meanwhile, the area of the perforate 221 of the second body 22 bottoms is less than the area of the second body 22 bottoms, and liquid any seepage can not occur.
Inverted round stage shape structure can better be protected the second body 22, prevents that the second body 22 is chipping.For further protecting the second body 22; not only the sidewall at groove 511 is provided with bolster 513; also at the lateral wall of the first body 21, be provided with stop block 211, the joint face of the lower limb of stop block 211 and the first body 21 and the second body 22 is in the same plane simultaneously.When fixture 51 rises while being fixedly connected with the second body, 211 pairs of working arms 512 of stop block stop, prevent that fixture from too rising, thereby the bottom of the second body 22 are squeezed and chipping.
In sum, the device of automatically preparing cell block that the utility model provides, the first conduit 43 in liquid taking device 4 and working barrel 44 add cell liquid to be prepared and the reagent that is arranged in reagent bottle to test tube 2 by mechanical arm 41 and suction pipe 42.Machinery is got liquid and has been replaced traditional liquid of manually getting, and reagent does not contact with operator, and operator's health can not come to harm.By controlling working barrel 44, also can reach the addition of controlling reagent, to compare precision higher with manual operation.By arrange pressue device 6 soak formalin, dehydration, dyeing, transparent and soak the steps such as paraffin in constantly repeat to increase malleation or negative pressure to test tube 2 bottoms, make to be positioned at cell on semi-permeable diaphragm 3 fully with the reagent reacting adding.User only need set the time that pressue device 6 adds malleation and negative pressure on demand, can automatically complete said process.
In addition, upper surface and lower surface at semi-permeable diaphragm 3 arrange wire netting 7, when heating arrangement is during to paraffin pipeline and test tube, wire netting 7 has good heat conduction function and the heat on test tube can be delivered on semi-permeable diaphragm 3, makes the paraffin on semi-permeable diaphragm 3 in soaking paraffin process, keep liquid.
Although the utility model is disclosed as above by preferred embodiment; yet not in order to limit the utility model; anyly know this skill person; within not departing from spirit and scope of the present utility model; can do a little change and retouching, therefore protection domain of the present utility model is when being as the criterion depending on claims scope required for protection.
Claims (8)
1. automatically prepare a device for cell block, it is characterized in that, comprising:
Rotating disk, has a plurality of the first fixed orifices;
Test tube, the quantity of described test tube is at least one, and is fixed on the first fixed orifice, and described test tube comprises the first body and the second body, and described the first body and the second body are detachable connection, and the bottom of described the second body has perforate;
Semi-permeable diaphragm, is arranged between described the first body and the second body;
Liquid taking device, for test tube adds cell liquid, reagent and whiteruss, comprising:
Mechanical arm, comprises bottom, rotation section and clamping part, and described bottom has a plurality of through holes, and described mechanical arm is hollow structure, and is provided with controller in described rotation section;
Suction pipe, is arranged at described clamping part;
The first conduit, is connected with described suction pipe through described through hole and mechanical arm inside, for the circulation of liquid;
Working barrel, is arranged at described the first conduit, the reagent that is positioned at the reagent bottle of described suction pipe below is drawn into described suction pipe, and a first conduit correspondence is provided with a working barrel; Wherein said working barrel and described controller are electrically connected;
Draw-out device, comprises fixture and the second conduit, and described fixture is hollow structure, and the perforate of the open top of described fixture and described the second body bottom is fixedly connected, and one end of the bottom opening of fixture and the second conduit is fixedly connected;
Pressue device, is connected with the other end of described the second conduit, by the second conduit, bottom test tube, provides malleation or negative pressure.
2. the device of automatically preparing cell block according to claim 1, is characterized in that, the upper surface of described semi-permeable diaphragm and lower surface are all coated with wire netting, and described wire netting and semi-permeable diaphragm are all arranged between described the first body and the second body.
3. the device of automatically preparing cell block according to claim 1, is characterized in that, on the sidepiece of described test tube, is provided with sensor, and described sensor electrically connects the controller in described rotation section, the liquid level of liquid in the real-time test tubes of sensor.
4. the device of automatically preparing cell block according to claim 1, is characterized in that, on described the first conduit, is provided with solenoid valve, and a first conduit correspondence is provided with a solenoid valve, and described solenoid valve and controller electric connection.
5. the device of preparing cell block according to claim 1, is characterized in that, described the second body is the inverted round stage shape of hollow, and the area that is positioned at the perforate of the second body bottom is less than the area of the second body bottom.
6. the device of automatically preparing cell block according to claim 5, is characterized in that, the top of described fixture is provided with the groove corresponding with the second body of hollow inverted round stage shape, and the both sides of described groove are provided with working arm.
7. the device of automatically preparing cell block according to claim 6, is characterized in that, the lateral wall of described the first body is provided with stop block, and the joint face of the lower limb of described stop block and described the first body and the second body is in the same plane.
8. the device of automatically preparing cell block according to claim 6, is characterized in that, the sidewall of described groove is provided with bolster.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420200440.7U CN203908836U (en) | 2014-04-23 | 2014-04-23 | Automatic device for preparing cell wax blocks |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420200440.7U CN203908836U (en) | 2014-04-23 | 2014-04-23 | Automatic device for preparing cell wax blocks |
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| Publication Number | Publication Date |
|---|---|
| CN203908836U true CN203908836U (en) | 2014-10-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420200440.7U Expired - Fee Related CN203908836U (en) | 2014-04-23 | 2014-04-23 | Automatic device for preparing cell wax blocks |
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| Country | Link |
|---|---|
| CN (1) | CN203908836U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103969096A (en) * | 2014-04-23 | 2014-08-06 | 杭州电子科技大学 | Automatic cell paraffin block preparation device and method |
-
2014
- 2014-04-23 CN CN201420200440.7U patent/CN203908836U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103969096A (en) * | 2014-04-23 | 2014-08-06 | 杭州电子科技大学 | Automatic cell paraffin block preparation device and method |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141029 Termination date: 20160423 |
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| CF01 | Termination of patent right due to non-payment of annual fee |