CN105028390A - Efficient biological material cryopreservation method - Google Patents
Efficient biological material cryopreservation method Download PDFInfo
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- CN105028390A CN105028390A CN201510433854.3A CN201510433854A CN105028390A CN 105028390 A CN105028390 A CN 105028390A CN 201510433854 A CN201510433854 A CN 201510433854A CN 105028390 A CN105028390 A CN 105028390A
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- hand basket
- shadoof
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Abstract
The invention relates to an efficient biological material cryopreservation method. According to the method, a device which is used comprises a nitrogen container lifting basket, a refrigeration support and casing pipes, the nitrogen container lifting basket is constituted by lifting baskets and a lifting rod, the lifting baskets comprise an upper lifting basket and a lower lifting basket, the lower lifting basket is fixedly connected with the lifting rod, the upper lifting basket is movably connected with the lifting rod, an upper location circular ring is arranged on the upper lifting basket, a lower location circular ring is arranged on the lower lifting basket, a location plug pin is placed inside the upper location circular ring and the lower location circular ring, the height of each lifting basket is 20-21 cm, the distance between the upper lifting basket and the lower lifting basket is 2-3 cm, the refrigeration support comprises semicircular supports and arc-shaped protruding pieces, one end of each semicircular support is provided with a retaining piece, the distance between each retaining piece and the opening end of the corresponding casing pipe is 1-2 cm, and the length of each semicircular support is 1-2 cm larger than the height of the corresponding lifting basket. By means of the device, the storage capacity of a liquid nitrogen container which is used currently can be increased by one time, the economic input for buying liquid nitrogen containers is reduced, and the cost is saved for a user.
Description
Technical field
The present invention relates to a kind of biomaterial cryopreservation methods efficiently.
Background technology
Vitrifying snap frozen is fused high concentration cryoprotector and embryo/ovum; after certain hour balance; embryo/ovum volume reduces 30-50% because of dehydration; afterwards dehydration embryo/ovum is inserted in freezing year bar; again the freezing year bar being loaded with dehydration embryo/ovum is directly dropped in liquid nitrogen and make dehydration cell instantaneous vitrification; form lower preservation without ice crystal, cause injury to cell to reduce intracellular ice.
Can not be damaged in refrigerating process by freezing year bar of loading dehydration cell in order to protect; and from liquid nitrogen container, easily take out the patient information simultaneously can also telling embryo/ovum; usually sample can be placed in freezing support; and freezing support also needs possess the effect of clamping freezing sleeve pipe; sleeve pipe is put into by freezing year bar; freezing support put into by sleeve pipe; again by the stentplacement in conjunction with freezing sleeve pipe in liquid nitrogen hand basket, liquid nitrogen hand basket is placed in liquid nitrogen container and carries out freezing in-196 degrees Celsius.
In present vitrifying embryo freezing method, the height of each liquid nitrogen container hand basket used is 40 centimetres (bottom hand basket hollow outs), freezing stent length is 42 centimetres, on freezing support, installation length is the sleeve pipe of 12 centimetres, because freezing stent length is longer, after single sleeve pipe is installed, distance between sleeve opening to freezing support catch is very large, year bar (length is 12 centimetres) being mounted with embryo can be deviate from from sleeve opening, therefore be adopt two sleeve pipes (as shown in Figure 7) of installing positive and negative mutual button on freezing support at present, prevent carrying bar and lose.The storage of current liquid nitrogen container used is highly 40 cm, and be therefore limited to the structure of above-mentioned liquid nitrogen container hand basket and freezing support, existing liquid nitrogen container only can store one deck embryo, and liquid nitrogen container utilization ratio is lower.And liquid nitrogen container is expensive, some liquid nitrogen container even needs unit up to ten thousand, therefore the availability improving liquid nitrogen container can reduce the equipment cost needing unit embryo being carried out to stored frozen.
Summary of the invention
The technical problem to be solved in the present invention is: provide the efficient biomaterial cryopreservation methods that a kind of liquid nitrogen container availability doubles, the frozen carrier arrangement of biomaterial that the method uses, the storage capacity of current liquid nitrogen container used can be made to double, solve above-mentioned prior art Problems existing.
The technical scheme solved the problems of the technologies described above is: a kind of biomaterial cryopreservation methods efficiently, this efficient biomaterial cryopreservation methods adopts the frozen carrier arrangement of biomaterial, the frozen carrier arrangement of described biomaterial comprises nitrogen tank hand basket, freezing support, sleeve pipe and year bar, described nitrogen tank hand basket is mainly formed by hand basket with the shadoof of hook, described shadoof is flat cuboid, described hand basket is the bucket body structure of upper end open bottom end seal, described hand basket comprises top hand basket and bottom hand basket, bottom hand basket is fixedly connected with shadoof, top hand basket is flexibly connected with shadoof, described top hand basket is provided with location annulus, bottom hand basket is provided with lower location annulus, fastening screw is placed with in upper location annulus and lower location annulus, the height H of described hand basket is 20 ~ 21cm, distance D1 between top hand basket and bottom hand basket is 2 ~ 3cm, described freezing support comprises semi-circular bracket and is arranged on the circular arc lug for fastening sleeve pipe at semi-circular bracket edge, one end of semi-circular bracket is provided with catch, distance D2 between this catch and ferrule openings end is 1 ~ 2cm, the length L1 1 ~ 2cm larger than the height H of hand basket of semi-circular bracket, the length L2 2 ~ 3cm less of the length L1 of semi-circular bracket of sleeve pipe,
The concrete operations of this efficient biomaterial cryopreservation methods are: first loaded by biomaterial and carry in bar, to carry bar is again placed in sleeve pipe, sleeve pipe to be placed in semi-circular bracket and by the fastening of circular arc lug, then semi-circular bracket is put into top hand basket or the bottom hand basket of nitrogen tank hand basket, then nitrogen tank hand basket is placed in liquid nitrogen container in-196 degrees Celsius of stored frozen.
Further technical scheme of the present invention is: described shadoof has through hole, and top hand basket is arranged on shadoof by the bending bar through shadoof through hole.
Another further technical scheme of the present invention is: described shadoof has through hole, top hand basket is provided with screw rod, and screw rod is passed the through hole of shadoof and fixed by nut.
Described top hand basket is also configured with lid.
Described semi-circular bracket is also provided with information signboard.
The efficient biomaterial cryopreservation methods of the present invention, main innovative point is the improvement of operative installations, namely keep with in the much the same situation of present level at hand basket height, the hand basket of original about 40 centimetres is improved to about two the 20 centimetres of little hand baskets arranged and (bottom each hand basket, does the end with stainless steel aluminium sheet, prevent liquid nitrogen from missing), bottom hand basket and shadoof are fixed, and top hand basket is flexibly connected with shadoof, namely top hand basket can rotate to the angle (as shown in Figure 4) vertical with shadoof, convenient when taking out the embryo in the hand basket of bottom, top hand basket can be rotated down and still be dipped in liquid nitrogen, to ensure the quality stored.Adopt this structure, the storage capacity that current liquid nitrogen pot fetching used can be made to double, avoids making a big purchase liquid nitrogen container in large quantities, reduces the economic input buying liquid nitrogen container, for applying unit has saved cost, also avoids adding the loss that a large amount of liquid nitrogen container causes liquid nitrogen.In addition, a large amount of liquid nitrogen containers needs large-area place to place, and the device that the present invention uses can save the placement place of liquid nitrogen container.
Below, in conjunction with the accompanying drawings and embodiments the technical characteristic of a kind of efficient biomaterial cryopreservation methods of the present invention is further described.
Accompanying drawing explanation
Fig. 1: the nitrogen tank hand basket right view that the present invention uses.
The A portion enlarged drawing of Fig. 2: Fig. 1.
Fig. 3: the nitrogen tank hand basket front view that the present invention uses.
Fig. 4: the nitrogen tank basket structure schematic diagram (top hand basket rotates to vertical with shadoof) that the present invention uses.
Fig. 5: the freezing support stereogram being fixed with sleeve pipe that the present invention uses.
Fig. 6: the freezing support front view being fixed with sleeve pipe that the present invention uses.
Fig. 7: existing freezing support and sleeve structure schematic diagram.
In figure: I-nitrogen tank hand basket, II-freezing support, III-sleeve pipe, 1-bottom hand basket, 2-top hand basket, 3-shadoof, 31-links up with, and 32-through hole, 4-locates annulus, 5-fastening screw, annulus is located, 7-bending bar, 8-semi-circular bracket, 81-catch, 9-circular arc lug under 6-.
Embodiment
Embodiment 1: a kind of biomaterial cryopreservation methods efficiently, this efficient biomaterial cryopreservation methods adopts the frozen carrier arrangement of biomaterial, as shown in figs 1 to 6, comprise nitrogen tank hand basket I, the freezing support II of aluminum, sleeve pipe III and year bar (not shown in FIG.), described nitrogen tank hand basket I is mainly formed by hand basket with the shadoof 3 of hook 31, described shadoof is flat cuboid, described hand basket is the bucket body structure of upper end open bottom end seal, described hand basket comprises top hand basket 2 and bottom hand basket 1, bottom hand basket 1 is fixedly connected with shadoof 3, top hand basket 2 is flexibly connected with shadoof 3, namely described shadoof 3 has through hole 32, top hand basket 2 is arranged on shadoof 3 by the bending bar 7 through shadoof through hole 32, top hand basket 2 can rotate around bending bar 7.Described top hand basket is provided with location annulus 4, bottom hand basket is provided with lower location annulus 6, in upper location annulus and lower location annulus, be placed with fastening screw 5, to ensure that hand basket can not rotate arbitrarily in stored frozen process.The height H of described hand basket is 20cm, distance D1 between top hand basket and bottom hand basket is 2cm, described freezing support II comprises semi-circular bracket 8 and is arranged on the circular arc lug 9 for fastening sleeve pipe III at semi-circular bracket edge, one end of semi-circular bracket is provided with catch 81, both can be used for stopping in use procedure and do not deviate from sleeve pipe for the bar that carries loading embryo, corresponding position mark can also be done on catch 81, distance D2 between this catch and ferrule openings end is 2cm, this distance can ensure length be 12 centimetres carry a bar can not deviate from semi-circular bracket, the length L1 of semi-circular bracket is 21cm, the length L2 of sleeve pipe is 18cm.
The concrete operations of this efficient biomaterial cryopreservation methods are: first embryo loaded and carry in bar, to carry bar is again placed in sleeve pipe III, sleeve pipe III to be placed in semi-circular bracket 8 and by circular arc lug 9 fastening, then several semi-circular bracket 8 is put into respectively top hand basket 2 or the bottom hand basket 1 of nitrogen tank hand basket I, then nitrogen tank hand basket I is placed in liquid nitrogen container in-196 degrees Celsius of stored frozen.When needing to take out the embryo in the hand basket of bottom, top hand basket being rotated to the angle (as shown in Figure 4) vertical with shadoof, is still immersed in liquid nitrogen, taking-up bottom hand basket embryo simultaneously, the stored frozen quality of top hand basket can be ensured like this.
In the present embodiment, colour-coded can be adopted on year bar to carry out embryonic marker, be conducive to finding required embryo at once.On same hand basket top just marks in embryo's record, bottom hand basket is labeled as down.After semi-circular bracket 8 is put into hand basket, can the position indicated residing for embryo be preferably top hand basket 2 or bottom hand basket 1 on hand basket.As one conversion, the regular marks can arranged according to constituent parts or each laboratory, as long as be convenient to find required embryo fast.
In the present embodiment, described top hand basket is also configured with aluminum lid.Described semi-circular bracket leaves blank space, is provided with information signboard, for marking embryo present position, easy-to-look-up.As one conversion, the structure of lid and information signboard also can not be set.
One as the present embodiment converts, and top hand basket 2 also can adopt other connected mode with the connected mode of shadoof 3, as: top hand basket is provided with screw rod, and screw rod is passed the through hole of shadoof and is fixed by nut.
One as the present embodiment converts, the size of each parts can also adjust according to actual conditions, the height H of general hand basket is 19 ~ 21cm, distance D1 between top hand basket and bottom hand basket is 2 ~ 3cm, distance D2 between catch and ferrule openings end is 1 ~ 2cm, the length L1 1 ~ 2cm larger than the height H of hand basket of semi-circular bracket, the length L2 2 ~ 3cm less of the length L1 of semi-circular bracket of sleeve pipe.
Within of the present invention year, bar structure is identical with of the prior art year bar, repeats no more herein.
Claims (5)
1. an efficient biomaterial cryopreservation methods, it is characterized in that: this efficient biomaterial cryopreservation methods adopts the frozen carrier arrangement of biomaterial, the frozen carrier arrangement of described biomaterial comprises nitrogen tank hand basket (I), freezing support (II), sleeve pipe (III) and year bar, described nitrogen tank hand basket (I) is mainly formed by hand basket with the shadoof (3) of hook (31), described shadoof is flat cuboid, described hand basket is the bucket body structure of upper end open bottom end seal, described hand basket comprises top hand basket (2) and bottom hand basket (1), bottom hand basket (1) is fixedly connected with shadoof (3), top hand basket (2) is flexibly connected with shadoof (3), described top hand basket is provided with location annulus (4), bottom hand basket is provided with lower location annulus (6), fastening screw (5) is placed with in upper location annulus and lower location annulus, the height H of described hand basket is 20 ~ 21cm, distance D1 between top hand basket and bottom hand basket is 2 ~ 3cm, described freezing support (II) comprises semi-circular bracket (8) and is arranged on the circular arc lug (9) for fastening sleeve pipe (III) at semi-circular bracket edge, one end of semi-circular bracket is provided with catch (81), distance D2 between this catch and ferrule openings end is 1 ~ 2cm, the length L1 1 ~ 2cm larger than the height H of hand basket of semi-circular bracket, the length L2 2 ~ 3cm less of the length L1 of semi-circular bracket of sleeve pipe,
The concrete operations of this efficient biomaterial cryopreservation methods are: first loaded by biomaterial and carry in bar, to carry bar is again placed in sleeve pipe (III), sleeve pipe (III) is placed in semi-circular bracket (8) also by circular arc lug (9) fastening, then semi-circular bracket (8) is put into top hand basket (2) or the bottom hand basket (1) of nitrogen tank hand basket (I), then nitrogen tank hand basket (I) is placed in liquid nitrogen container in-196 degrees Celsius of stored frozen.
2. the efficient biomaterial cryopreservation methods of one according to claim 1, is characterized in that: described shadoof (3) has through hole (32), and top hand basket (2) is arranged on shadoof (3) by the bending bar (7) through shadoof through hole (32).
3. the efficient biomaterial cryopreservation methods of one according to claim 1, is characterized in that: described shadoof (3) has through hole (32), top hand basket is provided with screw rod, and screw rod is passed the through hole of shadoof and fixed by nut.
4. the efficient biomaterial cryopreservation methods of the one according to any one of claim 1-3, is characterized in that: described top hand basket is also configured with lid.
5. the efficient biomaterial cryopreservation methods of the one according to any one of claim 1-3, is characterized in that: described semi-circular bracket is also provided with information signboard.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510433854.3A CN105028390B (en) | 2015-07-22 | 2015-07-22 | A kind of efficient embryo freezing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510433854.3A CN105028390B (en) | 2015-07-22 | 2015-07-22 | A kind of efficient embryo freezing method |
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| CN105028390A true CN105028390A (en) | 2015-11-11 |
| CN105028390B CN105028390B (en) | 2017-07-21 |
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| CN201510433854.3A Active CN105028390B (en) | 2015-07-22 | 2015-07-22 | A kind of efficient embryo freezing method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105918309A (en) * | 2016-05-30 | 2016-09-07 | 柳州市妇幼保健院 | Efficient frozen storage system for embryos |
| CN106144223A (en) * | 2016-08-17 | 2016-11-23 | 桂林医学院 | Rotary cryopreservation tube storage device |
| JP2018057336A (en) * | 2016-10-06 | 2018-04-12 | 株式会社北里コーポレーション | Canister set for biological cell-cryopreservation container |
| CN109287616A (en) * | 2018-10-12 | 2019-02-01 | 深圳中山泌尿外科医院 | A kind of double-layer scaffold of storing frozen embryo |
Citations (3)
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|---|---|---|---|---|
| US20090255288A1 (en) * | 2008-04-10 | 2009-10-15 | Yu Jia | Portable rack carrier device and the method of use |
| US20100263175A1 (en) * | 2009-04-17 | 2010-10-21 | Jui-Yin Lee | Fastener module |
| CN202514486U (en) * | 2012-02-16 | 2012-11-07 | 上海安集协康生物技术有限公司 | Container suitable for liquid nitrogen tank to store cells |
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2015
- 2015-07-22 CN CN201510433854.3A patent/CN105028390B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090255288A1 (en) * | 2008-04-10 | 2009-10-15 | Yu Jia | Portable rack carrier device and the method of use |
| US20100263175A1 (en) * | 2009-04-17 | 2010-10-21 | Jui-Yin Lee | Fastener module |
| CN202514486U (en) * | 2012-02-16 | 2012-11-07 | 上海安集协康生物技术有限公司 | Container suitable for liquid nitrogen tank to store cells |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105918309A (en) * | 2016-05-30 | 2016-09-07 | 柳州市妇幼保健院 | Efficient frozen storage system for embryos |
| CN106144223A (en) * | 2016-08-17 | 2016-11-23 | 桂林医学院 | Rotary cryopreservation tube storage device |
| JP2018057336A (en) * | 2016-10-06 | 2018-04-12 | 株式会社北里コーポレーション | Canister set for biological cell-cryopreservation container |
| CN109287616A (en) * | 2018-10-12 | 2019-02-01 | 深圳中山泌尿外科医院 | A kind of double-layer scaffold of storing frozen embryo |
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| CN105028390B (en) | 2017-07-21 |
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