CN104655448A - Method for collecting hypophysis and hypothalamo of goat - Google Patents
Method for collecting hypophysis and hypothalamo of goat Download PDFInfo
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- CN104655448A CN104655448A CN201510063061.7A CN201510063061A CN104655448A CN 104655448 A CN104655448 A CN 104655448A CN 201510063061 A CN201510063061 A CN 201510063061A CN 104655448 A CN104655448 A CN 104655448A
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Abstract
The invention discloses a method for collecting hypophysis and hypothalamo of a goat. By using the method, complete hypophysis and hypothalamo tissue samples with relatively low pollution degrees can be rapidly obtained after a cranial bone of the goat is split, the collecting efficiency for brain tissue samples is ensured, and defects that tissues such as hypophysis and hypothalamo are high in pollution degree and are relatively easily lost and the like in the prior art are overcome; and the method is suitable for collecting a great number of samples and is simple and rapid in operation. The method disclosed by the invention is simple and good in using effect.
Description
Technical field
The present invention relates to life science, especially a kind of collection goat pituitary and hypothalamic method.
Background technology
Goat hypophysis is the most important incretory of goat body, point frontal lobe and posterior lobe two parts.It secretes multiple hormone, as growth hormone, thyrotropic hormone, corticotropin, gonadotropic hormone, oxytocins, prolactin, melanocyte-stimulating hormone etc., can also preserve the antidiuretic hormone of hypothalamus secretion.These hormones play an important role to metabolism, growth, growth and reproduction etc.Hypothalamus is the high-level center regulating visceral motility under cerebral cortex, it connects visceral motility and other physiological activities, regulates body temperature, ingests, the important physiological function such as water balance, blood sugar and incretory are movable.Gather complete free of contamination hypophysis and hypothalamus, carry out Total RNAs extraction, have vital meaning to its follow-up research.
The collection of existing goat brain tissue sample, particularly to goat pituitary and hypothalamic acquisition method, mainly with a chainsaw or the head of longitudinally riving such as axe be average two parts, riving in part, find brain tissue as hypophysis, hypothalamus etc.The hypophysis that the method obtains and hypothalamus larger owing to being subject to mechanical damage, and split in semiosis and very easily brain tissue is mixed (as fragile tissue such as brain, hypothalamus, cerebellum, hypophysis), when splitting searching sample in half due to consuming time longer, sample is exposed to for a long time in air and causes seriously polluted, carries out the research such as follow-up test have destructive pollution for such as extracting brain tissue RNA.
Summary of the invention
Technical matters to be solved by this invention is to provide and a kind of gathers goat pituitary and hypothalamic method, its collecting efficiency is high, integrality good, comparatively low stain, be easy to operation, to overcome the deficiencies in the prior art.
The present invention is achieved in that and gathers goat pituitary and hypothalamic method, comprises the steps:
1) get the goat head of having butchered, with a chainsaw or axe perpendicular to the direction of goat nose, the skull of goat of laterally riving at ear front distance occipital condyle 2-4cm place, splits into bone 0.8-1.5cm, and can see brain tissue in head;
2) along splitting mouth, the bone on both sides being rived again, the part of riving being separated, directly exposing brain tissue;
3) push cerebellum aside with ophthalmic tweezers, expose brain stem and brain junction, make goat pituitary and hypothalamus visible;
4) cut with the connecting place of eye scissors along pituitary and funnel, namely obtain intact goat pituitary; Use eye scissors along root clip hypothalamus again.
After the pituitary that takes off or hypothalamus DEPC process water washing, with sterilizing masking foil parcel, put into Liquid nitrogen storage immediately; Or put into ice chest immediately with after the EP pipe splendid attire that RNA conserving liquid is housed after washing.
Compared with prior art, the present invention obtains complete, more oligosaprobic pituitary and inferior colliculus cerebral tissue sample after being rived by goat skull fast, ensure that the collecting efficiency of brain tissue's sample, and overcome pituitary and hypothalamus etc. in prior art and organize that pollution level is high, the comparatively easy defect such as loss, and be applicable to great amount of samples collection, and simple and quick.The inventive method is simple, and result of use is good.
Accompanying drawing explanation
Accompanying drawing 1 is incision site schematic diagram of the present invention;
In accompanying drawing 1: 1-cutting bone, 2-maxilla, 3-nasal bone, 4-frontal bone, 5-angle, 6-occipital condyle;
Accompanying drawing 2 is for extracting total serum IgE disparity map after distinct methods method collection goat brain tissue;
In accompanying drawing 2: the goat brain hypophysis of 1-embodiment collection carries total serum IgE; The goat hypothalamus of 2-embodiment collection carries total serum IgE; The goat brain hypophysis of 3-prior art collection carries total serum IgE; The goat hypothalamus of 4-prior art collection carries total serum IgE.
Embodiment
Embodiments of the invention: gather goat pituitary and hypothalamic method, comprise the steps:
1) get the goat head of having butchered, with a chainsaw or axe perpendicular to the direction of goat nose, the skull of goat of laterally riving at ear front distance occipital condyle 2-4cm place, splits into bone 0.8-1.5cm, and can see brain tissue in head;
2) along splitting mouth, the bone on both sides being rived again, the part of riving being separated, directly exposing brain tissue, visible brain and cerebellum;
3), with aseptic ophthalmic tweezers and the appropriate brain of eye scissors clip, after rinsing clean brain with DEPC process water, with sterilizing masking foil parcel, Liquid nitrogen storage is put into immediately;
4), with the aseptic appropriate cerebellum of eye scissors clip, after rinsing clean cerebellum with DEPC process water, with sterilizing masking foil parcel, Liquid nitrogen storage is put into immediately;
5) goat skull is held, with occipital condyle and nasal bone for axis, by goat end rotation 180 °, then push cerebellum aside with ophthalmic tweezers, expose brain stem and brain junction, now can see goat pituitary and hypothalamus, the goat pituitary likeness in form seed of Job's tears, is connected in hypothalamus by means of funnel;
6) cut along pituitary and funnel connecting place with aseptic eye scissors, namely obtain intact goat pituitary, after DEPC process water washing pituitary, with sterilizing masking foil parcel, put into Liquid nitrogen storage immediately;
7) hypothalamus be connected with goat pituitary is similar to the projection be connected, and with eye scissors after root clip hypothalamus, with DEPC process water washing pituitary, with sterilizing masking foil parcel, puts into Liquid nitrogen storage immediately.
The pituitary of embodiment goat and hypothalamus and the carrying out of conventionally taking out are contrasted: gather the RNA isolation kit that the pituitary of goat and hypothalamus carry out total serum IgE respectively and extract, operation is extracted kit instructions with reference to RNApure Tissue Kit animal tissue/cell RNA that health is century bio tech ltd and is carried out, after extraction, integrity detection contrast is carried out to carried RNA, comparing result display as shown in Figure 2, it is better compared with the scheme of prior art that total serum IgE integrality is put forward by the goat pituitary adopting the scheme of embodiment to obtain and hypothalamus institute, pollution level obviously reduces.
Claims (2)
1. gather goat pituitary and a hypothalamic method, it is characterized in that: comprise the steps:
1) get the goat head of having butchered, with a chainsaw or axe in the frontal bone position in the direction perpendicular to goat nose, the skull of goat of laterally riving at ear front distance occipital condyle 2-4cm place, splits into bone 0.8-1.5cm, and can see brain tissue in head;
2) along splitting mouth, the bone on both sides being rived again, the part of riving being separated, directly exposing brain tissue;
3) push cerebellum aside with ophthalmic tweezers, expose brain stem and brain junction, make goat pituitary and hypothalamus visible;
4) cut with the connecting place of eye scissors along pituitary and funnel, namely obtain intact goat pituitary; Use eye scissors along root clip hypothalamus again.
2. collection goat pituitary according to claim 1 and hypothalamic method, is characterized in that: after the pituitary that takes off or hypothalamus DEPC process water washing, with sterilizing masking foil parcel, puts into Liquid nitrogen storage immediately; Or put into ice chest immediately with after the EP pipe splendid attire that RNA conserving liquid is housed after washing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510063061.7A CN104655448A (en) | 2015-02-08 | 2015-02-08 | Method for collecting hypophysis and hypothalamo of goat |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510063061.7A CN104655448A (en) | 2015-02-08 | 2015-02-08 | Method for collecting hypophysis and hypothalamo of goat |
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| Publication Number | Publication Date |
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| CN104655448A true CN104655448A (en) | 2015-05-27 |
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| CN201510063061.7A Pending CN104655448A (en) | 2015-02-08 | 2015-02-08 | Method for collecting hypophysis and hypothalamo of goat |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106725973A (en) * | 2017-02-16 | 2017-05-31 | 临沂大学 | It is a kind of obtain complete sheep brain open cranium method |
| CN107019576A (en) * | 2017-03-28 | 2017-08-08 | 中国农业科学院北京畜牧兽医研究所 | Animal head fast anatomical and complete brain tissue's separation method |
| CN109612767A (en) * | 2018-12-30 | 2019-04-12 | 河南农业大学 | A method of acquisition pig pituitary and hypothalamus |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101451928A (en) * | 2007-12-05 | 2009-06-10 | 中国农业科学院北京畜牧兽医研究所 | Multifunction organization embedding and refrigerating plant |
-
2015
- 2015-02-08 CN CN201510063061.7A patent/CN104655448A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101451928A (en) * | 2007-12-05 | 2009-06-10 | 中国农业科学院北京畜牧兽医研究所 | Multifunction organization embedding and refrigerating plant |
Non-Patent Citations (3)
| Title |
|---|
| 岳建辉 等.: "《猪SOSC5和SOCS6基因克隆及组织表达研究》", 《四川大学学报(自然科学版)》 * |
| 张建明.: "《牛羊的病理剖检方法简介》", 《农业开发与装备》 * |
| 雷治海.: "《动物解剖学实验教程》", 31 January 2006, 《中国农业大学出版社》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106725973A (en) * | 2017-02-16 | 2017-05-31 | 临沂大学 | It is a kind of obtain complete sheep brain open cranium method |
| CN106725973B (en) * | 2017-02-16 | 2020-05-01 | 临沂大学 | Craniotomy method for obtaining complete sheep brain |
| CN107019576A (en) * | 2017-03-28 | 2017-08-08 | 中国农业科学院北京畜牧兽医研究所 | Animal head fast anatomical and complete brain tissue's separation method |
| CN109612767A (en) * | 2018-12-30 | 2019-04-12 | 河南农业大学 | A method of acquisition pig pituitary and hypothalamus |
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Application publication date: 20150527 |