CN106725973A - It is a kind of obtain complete sheep brain open cranium method - Google Patents
It is a kind of obtain complete sheep brain open cranium method Download PDFInfo
- Publication number
- CN106725973A CN106725973A CN201710083059.5A CN201710083059A CN106725973A CN 106725973 A CN106725973 A CN 106725973A CN 201710083059 A CN201710083059 A CN 201710083059A CN 106725973 A CN106725973 A CN 106725973A
- Authority
- CN
- China
- Prior art keywords
- brain
- cut
- line
- complete
- sheepshead
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Cranium method is opened the invention discloses a kind of complete sheep brain of acquisition, it is comprised the following steps：Remove complete sheepshead；A line, each line at the 2cm of left and right ear lower section are cut backward along calvarium center line in cutting skin line, a horizontal line at 2cm before goat's horn, longitudinal direction；Use excision 1cm skins wide along scalpel；Fixed sheepshead, is directed at each bar line and is cut using Arm saw, and depth of cut is 1cm or so；Chisel insertion cranium is determined into transverse cuts gap, by lever principle, cranium is opened；After complete exposure sheep brain, sheep brain is lifted slightly, using scalpel, progressively cut from front to back, obtain complete sheep brain；Ephippium cartilage is cut using scalpel, complete hypophysis is obtained.Cranium method is opened using of the invention, complete sheep brain and each organ-tissue of encephalic can be obtained, complete material is provided to make sample, with certain economic value；Simultaneously for scientific research provides excellent material.
Description
Technical field
The present invention relates to bioscience technology field, be specifically related to a kind of complete sheep brain of acquisition opens cranium method.
Background technology
Sheep is very important domestic animal, is one of main sources of physical resources such as our meat, hair, milk.The life of sheep
Long and development is closely related with hormone, but most of hormone both is from intracerebral, such as growth hormone.In scientific research process
In, in order to probe into the generation and metabolism of intracerebral hormone, it is necessary to obtain the complete tissue of intracerebral.Such as breeding and the rush of hypothalamus secretion
Gonadotropin releasing hormone is closely related, in order to study hypothalamus regulation and control gonadotropin-releasing hormone (GRH) mechanism of secretion, Wo Menbi
Complete inferior colliculus cerebral tissue must be obtained.Intracerebral also has some special samples such as hypophysis, pineal body to be also required to obtaining complete sheep
Could be obtained on the premise of brain.So how cleverly to open cranium, obtain complete tissue has very big value to scientific research.
On the other hand, during intracerebral sample is made, complete tissue, sample is also usually needed to have economic valency as commodity
Value, so obtaining complete sheep brain opens the method for cranium also with certain economic worth.
The method and technology that cranium performed the operation to be opened for people many at present, but the structure of sheep and people is completely different, and sheep
Or even many animals all do not have systematic method for opening cranium, so the cranium method of opening that we develop has novelty and advanced
Property.
The content of the invention
Present invention solves the technical problem that be, in view of the shortcomings of the prior art, there is provided it is a kind of obtain complete sheep brain open cranium
Method.
Technical scheme is as follows：
It is a kind of obtain complete sheep brain open cranium method, comprise the following steps：
S1：After butchering goat, in removing complete sheepshead in 1-60min, and with 0.01-12h in carry out out cranium and operate；
S2：Skin is cut using scalpel to rule：(eyes back upper place) cuts one at 2cm before the goat's horn of sheepshead calvarium
X wire, is brain forward position below the position；A vertical line is cut along calvarium center line (toward neck direction) backward in longitudinal direction；Left and right
Ear lower section place each line, respectively left line and right line, the left line and right line on neck obliquely by below ear with
X wire intersects；
S3：Using scalpel and scissors, transversely line, vertical line, left line and right line respectively cut off 1cm skins wide respectively, along
, it is necessary to some meat are cut off when left line and right wire cutting ear lower section；
S4：, can be put in sheepshead on stool by fixed sheepshead, and being directed at each bar line using Arm saw is cut, and cutting is deep
It is 1 ± 0.02cm to spend, preferably 1cm, saw calvarium when it is important to note that, it is impossible to cut through depth；
S5：By chisel insertion calvarium transverse cuts gap, by lever principle, cranium is opened, or to hold goat's horn past
Both sides are exerted oneself, and break sheepshead into two with one's hands；
S6：After complete exposure sheep brain, sheep brain is lifted slightly, using scalpel, progressively cut from front to back, obtain complete
Sheep brain；
S7：By sheep brain in turn, the hypothalamus of " peanut valve " shape is clear that, hypothalamus is careful with small scissors
Cut；
S8：Sheepshead front is put, is cut downwards along brain center line using scalpel, open brain, connected in brain and cerebellum
Position is connect it can be seen that a round bead, the sample is pineal body；
S9:Ephippium cartilage is cut using scalpel, is cut toward both sides as possible, finally take out complete hypophysis with scissors,
The hypophysis of taking-up also with cartilage and film, film and cartilage is carefully removed, and obtains complete pituitary；
S10：Sampling is completed, complete brain tissue, including brain, cerebellum, hypothalamus, pineal body, pituitary is obtained.
Further, in such scheme, after butchering goat, in removing complete sheepshead in 1-20min.
Further, in such scheme, after removing complete sheepshead, cranium operation is carried out out in 0.01-1h.
Further, in such scheme, at the brain that will remove, cerebellum, hypothalamus, pineal body, pituitary DEPC
After reason water washing, contained with the EP pipes equipped with RNA preservation liquid and preserved.
Alternatively, brain, cerebellum, hypothalamus, pineal body, the solution of the following formulas of pituitary that will be removed
Sealing immersion is preserved：Guanidine hydrochloride 22.15%, ferric pyrophosphate 18.2%, ferrous fumarate 2.88%, dithiothreitol (DTT) 0.328%,
Lysine hydrochloride 0.012%, N- acyl derivatives 0.65%, cryoprotector 2~5%, water add to 100%, and the freezing is protected
Shield agent is dimethyl sulfoxide (DMSO), and storage temperature is arranged on -40~-60 DEG C.
The beneficial effects of the invention are as follows：The present inventor by the careful observation and dissection to goat head construction,
Determine the location of skeletal structure, sheep brain of sheepshead, each bone tie point of sheepshead, formulate a set of acquisition complete brain tissue of sheep
Open cranium method, the method has certain systematicness, simple to operate, it is ensured that brain tissue is not destroyed, so that it is complete to obtain intracerebral
Whole tissue, including brain, cerebellum, hypothalamus, hypophysis, pineal body, not only facilitate scientific research, and making intracerebral mark
Present aspect has certain economic worth.
Brief description of the drawings
Fig. 1 is of the invention to cut and cross schematic diagram；
Fig. 2 is the present invention along each wire cutting skin schematic diagram；
Wherein, 1- x wires, 2- vertical lines, the left lines of 3-, the right lines of 4-.
Specific embodiment
Present aspect is further described in detail with reference to specific embodiment：
Embodiment 1：
It is a kind of obtain complete sheep brain open cranium method, comprise the following steps：
S1：After butchering goat, in removing complete sheepshead in 1min, and with 0.01h in carry out out cranium and operate；
S2：Skin is cut using scalpel to rule：(eyes back upper place) cuts one at 2cm before the goat's horn of sheepshead calvarium
X wire 1, is brain forward position below the position；A vertical line 2 is cut along calvarium center line (toward neck direction) backward in longitudinal direction；It is left
Each line, respectively left line 3 and right line 4 at auris dextra lower section, the left line 3 and right line 4 are on neck obliquely by ear
Lower section and x wire 1 are intersecting；
S3：Using scalpel and scissors, transversely line 1, vertical line 2, left line 3 and right line 4 respectively cut off 1cm skins wide respectively,
, it is necessary to some meat are cut off during along left line 3 and the cutting ear lower section of right line 4；
S4：, can be put in sheepshead on stool by fixed sheepshead, and being directed at each bar line using Arm saw is cut, and cutting is deep
It is 0.98cm to spend, saw calvarium when it is important to note that, it is impossible to cut through depth；
S5：By chisel insertion calvarium transverse cuts gap, by lever principle, cranium is opened, or to hold goat's horn past
Both sides are exerted oneself, and break sheepshead into two with one's hands；
S6：After complete exposure sheep brain, sheep brain is lifted slightly, using scalpel, progressively cut from front to back, obtain complete
Sheep brain；
S7：By sheep brain in turn, the hypothalamus of " peanut valve " shape is clear that, hypothalamus is careful with small scissors
Cut；
S8：Sheepshead front is put, is cut downwards along brain center line using scalpel, open brain, connected in brain and cerebellum
Position is connect it can be seen that a round bead, the sample is pineal body；
S9:Ephippium cartilage is cut using scalpel, is cut toward both sides as possible, finally take out complete hypophysis with scissors,
The hypophysis of taking-up also with cartilage and film, film and cartilage is carefully removed, and obtains complete pituitary；
S10：Sampling is completed, complete brain tissue, including brain, cerebellum, hypothalamus, pineal body, pituitary is obtained.
S11：After the brain that will remove, cerebellum, hypothalamus, pineal body, pituitary DEPC treatment water washings, with being equipped with
The EP pipes that RNA preserves liquid contain preservation.
Embodiment 2：
It is a kind of obtain complete sheep brain open cranium method, comprise the following steps：
S1：After butchering goat, in removing complete sheepshead in 20min, and with 1h in carry out out cranium and operate；
S2：Skin is cut using scalpel to rule：(eyes back upper place) cuts one at 2cm before the goat's horn of sheepshead calvarium
X wire 1, is brain forward position below the position；A vertical line 2 is cut along calvarium center line (toward neck direction) backward in longitudinal direction；It is left
Each line, respectively left line 3 and right line 4 at auris dextra lower section, the left line 3 and right line 4 are on neck obliquely by ear
Lower section and x wire 1 are intersecting；
S3：Using scalpel and scissors, transversely line 1, vertical line 2, left line 3 and right line 4 respectively cut off 1cm skins wide respectively,
, it is necessary to some meat are cut off during along left line 3 and the cutting ear lower section of right line 4；
S4：, can be put in sheepshead on stool by fixed sheepshead, and being directed at each bar line using Arm saw is cut, and cutting is deep
It is 1cm to spend, saw calvarium when it is important to note that, it is impossible to cut through depth；
S5：By chisel insertion calvarium transverse cuts gap, by lever principle, cranium is opened, or to hold goat's horn past
Both sides are exerted oneself, and break sheepshead into two with one's hands；
S6：After complete exposure sheep brain, sheep brain is lifted slightly, using scalpel, progressively cut from front to back, obtain complete
Sheep brain；
S7：By sheep brain in turn, the hypothalamus of " peanut valve " shape is clear that, hypothalamus is careful with small scissors
Cut；
S8：Sheepshead front is put, is cut downwards along brain center line using scalpel, open brain, connected in brain and cerebellum
Position is connect it can be seen that a round bead, the sample is pineal body；
S9:Ephippium cartilage is cut using scalpel, is cut toward both sides as possible, finally take out complete hypophysis with scissors,
The hypophysis of taking-up also with cartilage and film, film and cartilage is carefully removed, and obtains complete pituitary；
S10：Sampling is completed, complete brain tissue, including brain, cerebellum, hypothalamus, pineal body, pituitary is obtained.
S11：Brain, cerebellum, hypothalamus, pineal body, the solution sealing immersion guarantor of the following formulas of pituitary that will be removed
Deposit：Guanidine hydrochloride 22.15%, ferric pyrophosphate 18.2%, ferrous fumarate 2.88%, dithiothreitol (DTT) 0.328%, lysine hydrochloride
0.012%th, N- acyl derivatives 0.65%, dimethyl sulfoxide (DMSO) 3%, water add to 100%, and storage temperature is arranged on -60 DEG C.
Embodiment 3：
It is a kind of obtain complete sheep brain open cranium method, comprise the following steps：
S1：After butchering goat, in removing complete sheepshead in 60min, and with 12h in carry out out cranium and operate；
S2：Skin is cut using scalpel to rule：(eyes back upper place) cuts one at 2cm before the goat's horn of sheepshead calvarium
X wire 1, is brain forward position below the position；A vertical line 2 is cut along calvarium center line (toward neck direction) backward in longitudinal direction；It is left
Each line, respectively left line 3 and right line 4 at auris dextra lower section, the left line 3 and right line 4 are on neck obliquely by ear
Lower section and x wire 1 are intersecting；
S3：Using scalpel and scissors, transversely line 1, vertical line 2, left line 3 and right line 4 respectively cut off 1cm skins wide respectively,
, it is necessary to some meat are cut off during along left line 3 and the cutting ear lower section of right line 4；
S4：, can be put in sheepshead on stool by fixed sheepshead, and being directed at each bar line using Arm saw is cut, and cutting is deep
It is 1.02cm to spend, saw calvarium when it is important to note that, it is impossible to cut through depth；
S5：By chisel insertion calvarium transverse cuts gap, by lever principle, cranium is opened, or to hold goat's horn past
Both sides are exerted oneself, and break sheepshead into two with one's hands；
S6：After complete exposure sheep brain, sheep brain is lifted slightly, using scalpel, progressively cut from front to back, obtain complete
Sheep brain；
S7：By sheep brain in turn, the hypothalamus of " peanut valve " shape is clear that, hypothalamus is careful with small scissors
Cut；
S8：Sheepshead front is put, is cut downwards along brain center line using scalpel, open brain, connected in brain and cerebellum
Position is connect it can be seen that a round bead, the sample is pineal body；
S9:Ephippium cartilage is cut using scalpel, is cut toward both sides as possible, finally take out complete hypophysis with scissors,
The hypophysis of taking-up also with cartilage and film, film and cartilage is carefully removed, and obtains complete pituitary；
S10：Sampling is completed, complete brain tissue, including brain, cerebellum, hypothalamus, pineal body, pituitary is obtained.
S11：Brain, cerebellum, hypothalamus, pineal body, the solution sealing immersion guarantor of the following formulas of pituitary that will be removed
Deposit：Guanidine hydrochloride 22.15%, ferric pyrophosphate 18.2%, ferrous fumarate 2.88%, dithiothreitol (DTT) 0.328%, lysine hydrochloride
0.012%th, N- acyl derivatives 0.65%, dimethyl sulfoxide (DMSO) 5%, water add to 100%, and storage temperature is arranged on -40 DEG C.
In the above description, the present invention is illustrated using preferably implementation method, but the present invention is not limited to above-mentioned reality
Mode is applied, various changes can be without departing from the spirit and scope of the invention carried out certainly.
Claims (5)
1. it is a kind of obtain complete sheep brain open cranium method, it is characterised in that comprise the following steps：
S1：After butchering goat, in removing complete sheepshead in 1-60min, and with 0.01-12h in carry out out cranium and operate；
S2：Skin is cut using scalpel to rule：An x wire (1), the position are cut at 2cm before the goat's horn of sheepshead calvarium
It is below brain forward position；A vertical line (2) is cut backward along calvarium center line in longitudinal direction；Each line at the ear lower section of left and right, respectively
For left line (3) and right line (4), the left line (3) and right line (4) are on neck obliquely by below ear and x wire (1)
It is intersecting；
S3：Using scalpel and scissors, transversely each excision 1cm of line (1), vertical line (2), left line (3) and right line (4) is wide respectively
Skin, it is necessary to some meat are cut off during along left line (3) and right line (4) cutting ear lower section；
S4：Fixed sheepshead, is directed at each bar line and is cut using Arm saw, and depth of cut is 1 ± 0.02cm；
S5：By chisel insertion calvarium transverse cuts gap, by lever principle, cranium is opened, or hold goat's horn toward both sides
Firmly, sheepshead is broken into two with one's hands；
S6：After complete exposure sheep brain, sheep brain is lifted slightly, using scalpel, progressively cut from front to back, obtain complete sheep
Brain；
S7：By sheep brain in turn, the hypothalamus of " peanut valve " shape is clear that, hypothalamus is carefully cut with small scissors
Under；
S8：Sheepshead front is put, is cut downwards along brain center line using scalpel, brain is opened, in brain and cerebellum connection position
Put it can be seen that a round bead, the sample is pineal body；
S9:Ephippium cartilage is cut using scalpel, is cut toward both sides as possible, finally take out complete hypophysis with scissors, taken out
Hypophysis also with cartilage and film, film and cartilage are carefully removed, obtain complete pituitary；
S10：Sampling is completed, complete brain tissue, including brain, cerebellum, hypothalamus, pineal body, pituitary is obtained.
2. it is as claimed in claim 1 it is a kind of obtain complete sheep brain open cranium method, it is characterised in that after butchering goat, in 1-
Complete sheepshead is removed in 20min.
3. it is as claimed in claim 1 it is a kind of obtain complete sheep brain open cranium method, it is characterised in that remove complete sheepshead
Afterwards, cranium operation is carried out out in 0.01-1h.
4. it is as claimed in claim 1 it is a kind of obtain complete sheep brain open cranium method, it is characterised in that it is the brain that will remove, small
After brain, hypothalamus, pineal body, pituitary process water washing with DEPC, contained with the EP pipes equipped with RNA preservation liquid and preserved.
5. it is as claimed in claim 1 it is a kind of obtain complete sheep brain open cranium method, it is characterised in that it is the brain that will remove, small
Brain, hypothalamus, pineal body, pituitary seal immersion and preserve with the solution of following formulas：Guanidine hydrochloride 22.15%, ferric pyrophosphate
18.2%th, ferrous fumarate 2.88%, dithiothreitol (DTT) 0.328%, lysine hydrochloride 0.012%, N- acyl derivatives
0.65%th, cryoprotector 2-5%, water add to 100%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710083059.5A CN106725973B (en) | 2017-02-16 | 2017-02-16 | Craniotomy method for obtaining complete sheep brain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710083059.5A CN106725973B (en) | 2017-02-16 | 2017-02-16 | Craniotomy method for obtaining complete sheep brain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106725973A true CN106725973A (en) | 2017-05-31 |
| CN106725973B CN106725973B (en) | 2020-05-01 |
Family
ID=58957570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710083059.5A Active CN106725973B (en) | 2017-02-16 | 2017-02-16 | Craniotomy method for obtaining complete sheep brain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106725973B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109612767A (en) * | 2018-12-30 | 2019-04-12 | 河南农业大学 | A method of acquisition pig pituitary and hypothalamus |
| CN113229981A (en) * | 2021-05-08 | 2021-08-10 | 西安交通大学医学院第一附属医院 | Cutting device |
| CN115778607A (en) * | 2022-11-29 | 2023-03-14 | 江苏乾宝牧业有限公司 | Method for quickly dissecting animal skull and separating complete brain tissue |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1864637A (en) * | 2005-05-18 | 2006-11-22 | 湖南加华生物科技发展有限公司 | Cow brain tissue sampling method |
| CN103380768A (en) * | 2013-07-05 | 2013-11-06 | 河南科技大学 | Manufacturing method of adult ram nervous system specimen |
| CN104655448A (en) * | 2015-02-08 | 2015-05-27 | 贵州大学 | Method for collecting hypophysis and hypothalamo of goat |
| CN104655449A (en) * | 2015-02-08 | 2015-05-27 | 贵州大学 | Method for collecting hypophysis and hypothalamo of pig |
-
2017
- 2017-02-16 CN CN201710083059.5A patent/CN106725973B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1864637A (en) * | 2005-05-18 | 2006-11-22 | 湖南加华生物科技发展有限公司 | Cow brain tissue sampling method |
| CN103380768A (en) * | 2013-07-05 | 2013-11-06 | 河南科技大学 | Manufacturing method of adult ram nervous system specimen |
| CN104655448A (en) * | 2015-02-08 | 2015-05-27 | 贵州大学 | Method for collecting hypophysis and hypothalamo of goat |
| CN104655449A (en) * | 2015-02-08 | 2015-05-27 | 贵州大学 | Method for collecting hypophysis and hypothalamo of pig |
Non-Patent Citations (1)
| Title |
|---|
| 雷治海: "《动物解剖学实验教程》", 31 January 2006, 北京：中国农业大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109612767A (en) * | 2018-12-30 | 2019-04-12 | 河南农业大学 | A method of acquisition pig pituitary and hypothalamus |
| CN113229981A (en) * | 2021-05-08 | 2021-08-10 | 西安交通大学医学院第一附属医院 | Cutting device |
| CN115778607A (en) * | 2022-11-29 | 2023-03-14 | 江苏乾宝牧业有限公司 | Method for quickly dissecting animal skull and separating complete brain tissue |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106725973B (en) | 2020-05-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106725973A (en) | It is a kind of obtain complete sheep brain open cranium method | |
| Pérez et al. | Gender and slaughter weight effects on carcass quality traits of suckling lambs from four different genotypes | |
| CN104872109B (en) | A kind of manufacture method of zoological specimens | |
| ES2836510T3 (en) | Method to wash and detoxify fresh animal liver and to carry out the preservation of cellular tissues | |
| US9839223B2 (en) | Fish processing systems and methods | |
| CN107019576A (en) | Animal head fast anatomical and complete brain tissue's separation method | |
| NO20130253A1 (en) | Dressing process for cultivated salmon to quickly induce unconsciousness during hosting | |
| Suneja et al. | Multi-technique approach to characterise the effects of cryopreservation on larval development of the Pacific oyster (Crassostrea gigas) | |
| CN102715567A (en) | Method for tenderizing muscles of squid carcass by ultra-high pressure | |
| Sheller et al. | Maintenance and synthesis of proteins for an anucleate axon | |
| CN109612767A (en) | A method of acquisition pig pituitary and hypothalamus | |
| JP4255089B1 (en) | Blood removal method for live fish | |
| CN205087344U (en) | Biological tissue freezes and deposits container | |
| JP2003190191A (en) | Animal fixation implement | |
| JP3706879B2 (en) | Method for suppressing movement function of live fish and method for preserving live fish subjected to suppression treatment | |
| Akbulut et al. | The correlation between renal tissue oxidative stress parameters and TNF-alpha levels in an experimental model of ischemia-reperfusion injury in mice | |
| Leigh et al. | Use of ‘thoracic stick’in halal slaughter of bobby calves | |
| ES2362063B1 (en) | PROCEDURE FOR OBTAINING A GELIFICABLE MIOFIBRILLARY EXTRACT FROM FISH REMAINS. | |
| CN202490013U (en) | Large deformation sternum connector | |
| CN108157350A (en) | A kind of method for making meiofauna skeleton specimen | |
| Omar et al. | Comparative morphological studies of otoliths (ear stones or crystals) in some marine and fresh water fishes | |
| JP3729844B2 (en) | Method for suppressing movement function of live fish and method for preserving live fish subjected to suppression treatment | |
| Dewell et al. | Description and justification of a consistent technique for euthanasia of bovines using firearm and penetrating captive bolt | |
| Gapat et al. | Diagnosis and therapeutic management of leukoderma in buffaloes | |
| Hornaday | How to collect mammal skins for purposes of study and for mounting |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |