CN202465712U - Stem cell isolated culturing device - Google Patents
Stem cell isolated culturing device Download PDFInfo
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- CN202465712U CN202465712U CN 201220083863 CN201220083863U CN202465712U CN 202465712 U CN202465712 U CN 202465712U CN 201220083863 CN201220083863 CN 201220083863 CN 201220083863 U CN201220083863 U CN 201220083863U CN 202465712 U CN202465712 U CN 202465712U
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 35
- 238000012258 culturing Methods 0.000 title abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 47
- 238000000926 separation method Methods 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 18
- 239000012528 membrane Substances 0.000 claims description 13
- 239000000835 fiber Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 239000012858 resilient material Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 17
- 230000009471 action Effects 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract 1
- 210000001178 neural stem cell Anatomy 0.000 description 16
- 238000000034 method Methods 0.000 description 14
- 230000001537 neural effect Effects 0.000 description 13
- 235000015097 nutrients Nutrition 0.000 description 10
- 238000004113 cell culture Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 208000037887 cell injury Diseases 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000002490 cerebral effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000001415 gene therapy Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 241001269238 Data Species 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000004992 fission Effects 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000007917 intracranial administration Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 210000000944 nerve tissue Anatomy 0.000 description 1
- 230000007472 neurodevelopment Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell extraction device belonging to the field of medicine equipment and more concretely provides a stem cell isolated culturing device comprising a culturing device, a filtering device and a centrifuging device, wherein the upper end of the culturing device is provided with a close cover; the filtering device and the culturing device are of a filtering film provided with lifting handles at two ends, and the filtering film is matched with the culturing device in shape; the culturing device is connected with the centrifuging device; and when the culturing device is used, the filtering device is placed in the culturing device and clings to the bottom wall of the culturing device, and the close cover is not sealed under the action of the lifting handles.
Description
Technical field
The utility model relates to a kind of biochemical test device, particularly a kind of stem cell separation and Culture device.
Background technology
Stem cell is meant the initiating cell with self and multidirectional differentiation potential, is the cells of origin of body, and can break up under given conditions becomes the cell that one or more constitute tissue or organ.Self is meant that stem cell can keep the size of stem cell colony through cell fission; Multidirectional differentiation is meant that stem cell has plasticity-, under different environment, can be divided into different histocyte (can be divided into neurocyte, scleroblast, myocardial cell etc. like mescenchymal stem cell) thereby the histoorgan of the various complicacies of formation body.
(Neural Stem Cell NSCs) is a kind of of stem cell to NSC, and NSC plays a significant role with repairing in the damaged nerve tissue at neurodevelopment.Neural stem cells transplantation is effective ways of repairing and replace impaired cerebral tissue, can rebuild part loop and function.NSC can be used as genophore in addition, is used for the gene therapy of intracranial tumors and other sacred disease, utilizes NSC as gene therapy vector, has remedied the some shortcomings of virus vector.In addition, NSC also can be used as toxicity and the pharmacological action that a kind of cell model is estimated new drug.
Along with the research to the NSC Application Areas is goed deep into, the vitro culture of NSC also becomes one of focus of studying instantly with separating.At present the stripping technique of NSC has two kinds: 1. Maitland culture: the single cell suspension suspension culture in petridish after nervous tissue digestion is disperseed; Forming goes down to posterity behind the neural ball (about 200 microns of diameters) cultivates several times and separates NSC (the Experimental Neurology 156 that obtains; 71-83,1999).This method need rely on the neural ball of hand picking, very inconvenience in mass-producing is cultivated.2.FACS method: the specific antigens to NSC carries out fluorescent mark earlier, screens stem cell with precision instrument.Though this method can obtain the stem cell of higher degree; But stem cell there is certain infringement; And need expensive precision instrument (Proceeding of the National Academy of Sciences of the United States of America 97; 14720-14725,2000).
In sum, study and develop a kind of separation purity NSC separation and Culture device high and with low cost, easy to operate, that be convenient to robotization and mass-producing cultivation and become focus instantly.
The utility model content
The utility model discloses a kind of stem cell separation and Culture device; To overcome the existing artificial deficiency of extracting stem cell; Provide a kind of be used for the more stdn of laboratory or medical institutions, cell preparation applicable industry circle, rapidly and efficiently, stem cell separation and Culture device that use range is wider, make that laboratory, hospital or factory can safe mass-producing ground high-level efficiency and prepare stem cell in high quality.
More specifically; The purpose of the utility model is; Adopt membrane filtration technique, the existing NSC separation and Culture technology of improvement provides the stem cell separation and Culture device that a kind of separation purity is high and with low cost, easy and simple to handle, be convenient to the automation size cultivation.This device constitutes an automatic successive stem cell separation and Culture device with NSC from embryo cerebral tissue extraction separation culturing process.
A kind of stem cell separation and Culture device that the utility model provides comprises culture apparatus, filtration unit and centrifugal device, it is characterized in that:
Said culture apparatus upper end is provided with a capping;
Said filtration unit and said culture apparatus are the filtering membrane that two ends are provided with handle, said filtering membrane shape and said culture apparatus form fit;
Said culture apparatus links to each other with said centrifugal device;
When said culture apparatus used, said filtration unit was put into said culture apparatus and is close to said culture apparatus diapire, and said handle makes said capping blow-by.
Preferably, described filtration unit links to each other with a fluid thruster, and said liquid propeller is used to advance the sepn process of neural ball.
Preferably, the handle of the filtration unit of said culture apparatus is a resilient material handle, the height of highly big and said culture apparatus sidewall, and thickness is 2mm-5mm.
Preferably, the filtering membrane of described filtration unit is a fiber membrane, and pore size filter is the 100-200 micron.
Preferably, described culture apparatus bottom is provided with a liquid outlet, and said liquid outlet is provided with valve.
Preferably, the liquid outlet of described culture apparatus links to each other with described centrifugal device.
The beneficial effect of the utility model is: after the utility model adopts said structure; Distinctive elastic performance of the handle of filtration unit and thickness can make culture apparatus the capping blow-by but leave the slit; And gap size can be through changing the thickness adjustment of handle, can avoid the too tight or too loose of manual work lid that the spiral cover sealing mode of conventional culture apparatus brings and damage when bringing cell cultures; When the large diameter neural ball of some amount (diameter is greater than 200 microns) can appear in filtration unit in culture apparatus, the disposable neural ball of dispersive that need digest once more shifted out culture apparatus and carries out next step operation and avoid the troublesome operation of the neural ball of hand picking and reduce by the centrifugal cell injury of bringing; The capping of culture apparatus is opened convenient, the observation of uncapping at any time in the time of can being convenient to cell cultures; The liquid outlet of culture apparatus directly links to each other with centrifugal device and can avoid cell culture fluid to pollute; Through the fluid-propelled effect of liquid propeller, can guarantee that the sepn process of neural ball is complete fast; Can drop into once more in another culture apparatus that filtration unit is housed after cell centrifugation in the nutrient solution is collected and cultivate; Be that filtration unit can shift out culture apparatus with it after nutrient solution is cultivated the neural ball that need to shift out appear in for some time; Cell in the nutrient solution can centrifugal collection continued be cultivated, and so circulation repeatedly.This device is cultivated according to different cells and is required a plurality of culture apparatuses that filtration unit is housed are set, and to simplify the manual operation process in the cell cultivation process greatly, greatly reduce material and pollute, and the reaction process stdn is controlled; The described device of the utility model is easy to operate effectively to can greatly improve production efficiency, and its preparation technology is simple, and convenient quality control is more suitable for scale operation and commercial applications; Use range is wide especially, therefore applies easily, can have a tremendous social and economic benefits in the short period of time.
Description of drawings
Fig. 1 is the utility model stem cell separation and Culture schematic representation of apparatus.
Embodiment
The utility model has been studied existing medical mechanical skill, and a kind of new stem cell separation and Culture device and purposes are provided.
The utility model ultimate demand makes stem cell separation and Culture device product and uses, and will enumerate embodiment below and further specify.Provide material and the specification of shape, the filtration unit handle of culture apparatus, type or model and method of use and some experimental study contents of centrifugal device by aforementioned summary of the invention in above-mentioned some experimental datas that provide and the following example; But should be appreciated that the utility model is not limited in listed research contents here; Should also be appreciated that term as used herein only is used to describe certain embodiments, and be not qualification the utility model.
Embodiment 1 stem cell separation and Culture apparatus structure and method of use thereof
The stem cell separation and Culture apparatus structure that is provided in order more clearly to describe the utility model below specifies conjunction with figs..Accompanying drawing 1 is the structural representation of the stem cell separation and Culture device of the utility model.
A kind of stem cell separation and Culture device that the utility model provides comprises culture apparatus (1), filtration unit (2) and centrifugal device (3), and wherein: culture apparatus upper end is provided with a capping (4), and the bottom is provided with a liquid outlet (5), and liquid outlet is provided with valve (6); Filtration unit is the filtering membrane (8) that two ends are provided with handle (7), and filtration unit links to each other with liquid propeller (9), and the liquid outlet of culture apparatus links to each other with centrifugal device; Handle is a resilient material handle, the height of highly big and said culture apparatus sidewall, and thickness is 3mm; The filtering membrane of filtration unit is a fiber membrane, and pore size filter is 200 microns.
The device that the utility model provides according to the separation and Culture requirement of NSC, is provided with 4-5 the culture apparatus circulation that filtration unit is housed when operation, wherein the filtering membrane of filtration unit and culture apparatus mate and be close to the culture apparatus diapire; When cultivating first; The filter membrane that will have handle is placed the culture apparatus bottom; Be close to the culture apparatus diapire, (it is collapsible curved to culture apparatus that concentration 10^5 cell/ml) drop in the culture apparatus, the handle of filtration unit are higher than the part of culture apparatus sidewall for the single cell suspension that will from the tire brain, prepare; The culture apparatus capping is closed, and whole culture apparatus will leave the slit identical with handle thickness; With behind the culture apparatus liquid outlet valve closes with culture apparatus put into incubator cultivate 4-6 days (37 ℃, 5%CO
2) the back taking-up, discarded nutrient solution is first put into another culture apparatus again with the filtration unit taking-up and is added 37 ℃ of enzyme reaction solutions (trypsinase 0.05%, DNase I 0.001%), 5%CO under the liquid propeller effect
2Reaction is 15 minutes under the condition, adds serum and stops digestion, opens the liquid outlet valve then and makes nutrient solution get into centrifugal device; The centrifugal 5min of 1000rpm abandons supernatant, adds nutrient solution; Piping and druming is for several times resuspended with centrifugal gained cell precipitation, from centrifugal device, takes out behind the formation cell suspension and is put into subculture in the culture apparatus, and cultural method is with cultivating first; Once more filtration unit is taken out, behind the medium centrifugal that obtains once more with cell precipitation with the resuspended the same cultivation of going down to posterity repeatedly afterwards of nutrient solution.
The above-described preferred embodiment of utilizing specifies the utility model, and the scope of unrestricted the utility model.Those skilled in the art can be through after reading the utility model; Make trickle change; For example: the type of the material of the shape of culture apparatus, filtration unit handle and specification, centrifugal device or model or the like; Will the can yet be regarded as main idea place of the utility model does not also break away from the spirit and the scope of the utility model.
In sum; The utility model provides a kind of new stem cell separation and Culture Design of device; Distinctive elastic performance of the handle of filtration unit and thickness can make culture apparatus the capping blow-by but leave the slit; And gap size can be through changing the thickness adjustment of handle, can avoid the too tight or too loose of manual work lid that the spiral cover sealing mode of conventional culture apparatus brings and damage when bringing cell cultures; When the large diameter neural ball of some amount (diameter is greater than 200 microns) can appear in filtration unit in culture apparatus, the disposable neural ball of dispersive that need digest once more shifted out culture apparatus and carries out next step operation and avoid the neural ball of hand picking and reduce by the centrifugal cell injury of bringing; Through the fluid-propelled effect of liquid propeller, can guarantee that the sepn process of neural ball is complete fast; The capping of culture apparatus is opened convenient, the observation of uncapping at any time in the time of can being convenient to cell cultures; The liquid outlet of culture apparatus directly links to each other with centrifugal device and can avoid cell culture fluid to pollute; Can drop into once more in another culture apparatus that filtration unit is housed after cell centrifugation in the nutrient solution is collected and cultivate; Be that filtration unit can shift out culture apparatus with it after nutrient solution is cultivated the neural ball that need to shift out appear in for some time; Cell in the nutrient solution can centrifugal collection continued be cultivated, and so circulation repeatedly.This device is cultivated according to different cells and is required a plurality of culture apparatuses that filtration unit is housed are set, and to simplify the manual operation process in the cell cultivation process greatly, greatly reduce material and pollute, and the reaction process stdn is controlled; The described device of the utility model is easy to operate effectively to can greatly improve production efficiency, and its preparation technology is simple, and convenient quality control is more suitable for scale operation and commercial applications; Use range is wide especially, therefore applies easily, can have a tremendous social and economic benefits in the short period of time.
Claims (6)
1. a stem cell separation and Culture device comprises culture apparatus (1), filtration unit (2) and centrifugal device (3), it is characterized in that:
Said culture apparatus upper end is provided with a capping (4);
Said filtration unit and said culture apparatus are the filtering membrane (8) that two ends are provided with handle (7), said filtering membrane shape and said culture apparatus form fit;
Said culture apparatus links to each other with said centrifugal device;
When said culture apparatus used, said filtration unit was put into said culture apparatus and is close to said culture apparatus diapire, and said handle makes said capping blow-by.
2. stem cell tripping device as claimed in claim 1 is characterized in that: described filtration unit links to each other with a fluid thruster (9).
3. stem cell separation and Culture device as claimed in claim 1 is characterized in that: the handle of said filtration unit is a resilient material handle, and height is greater than the height of said culture apparatus sidewall, and thickness is 2mm-5mm.
4. stem cell separation and Culture device as claimed in claim 3, the filtering membrane of the filtration unit of described stem cell device is a fiber membrane, pore size filter is the 100-200 micron.
5. stem cell separation and Culture device as claimed in claim 4, described culture apparatus bottom is provided with a liquid outlet (5), and said liquid outlet is provided with valve (6).
6. stem cell separation and Culture device as claimed in claim 5, the liquid outlet of described culture apparatus links to each other with centrifugal device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220083863 CN202465712U (en) | 2012-03-07 | 2012-03-07 | Stem cell isolated culturing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220083863 CN202465712U (en) | 2012-03-07 | 2012-03-07 | Stem cell isolated culturing device |
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| Publication Number | Publication Date |
|---|---|
| CN202465712U true CN202465712U (en) | 2012-10-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201220083863 Expired - Lifetime CN202465712U (en) | 2012-03-07 | 2012-03-07 | Stem cell isolated culturing device |
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| CN (1) | CN202465712U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603870A (en) * | 2017-11-10 | 2018-01-19 | 上海保兴生物设备工程有限公司 | A kind of digestion filter for micro-carriers cell culture |
-
2012
- 2012-03-07 CN CN 201220083863 patent/CN202465712U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603870A (en) * | 2017-11-10 | 2018-01-19 | 上海保兴生物设备工程有限公司 | A kind of digestion filter for micro-carriers cell culture |
| CN107603870B (en) * | 2017-11-10 | 2023-06-02 | 上海保兴生物设备工程有限公司 | Digestion and filtration device for microcarrier cell culture |
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| C14 | Grant of patent or utility model | ||
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| C56 | Change in the name or address of the patentee |
Owner name: SHANGHAI ANGECON BIO-TECHNOLOGY CO., LTD. Free format text: FORMER NAME: SHANGHAI ANGECON BIOTECHNOLOGY CO., LTD. |
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| CP03 | Change of name, title or address |
Address after: 201318 Shanghai City, Pudong New Area Road No. 588 building 3 indigo Patentee after: SHANGHAI ANGECON BIOTECHNOLOGY CO., LTD. Address before: 201203 Shanghai city Pudong New Area Zhangjiang High Tech Park of Shanghai Cailun Road Lane 720 Building No. 2 room 504 Patentee before: Shanghai Angecon Biotechnology Co., Ltd. |
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