CN208768813U - A kind of cell cryopreservation tube - Google Patents
A kind of cell cryopreservation tube Download PDFInfo
- Publication number
- CN208768813U CN208768813U CN201822209879.7U CN201822209879U CN208768813U CN 208768813 U CN208768813 U CN 208768813U CN 201822209879 U CN201822209879 U CN 201822209879U CN 208768813 U CN208768813 U CN 208768813U
- Authority
- CN
- China
- Prior art keywords
- tube
- bulge loop
- tube body
- pipe lid
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 42
- 239000000463 material Substances 0.000 claims abstract description 21
- 238000009413 insulation Methods 0.000 claims abstract description 19
- 238000007789 sealing Methods 0.000 claims abstract description 17
- 238000003306 harvesting Methods 0.000 claims abstract description 15
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 6
- GQWNECFJGBQMBO-UHFFFAOYSA-N Molindone hydrochloride Chemical compound Cl.O=C1C=2C(CC)=C(C)NC=2CCC1CN1CCOCC1 GQWNECFJGBQMBO-UHFFFAOYSA-N 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 22
- 239000007788 liquid Substances 0.000 abstract description 11
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 11
- 238000007710 freezing Methods 0.000 abstract description 7
- 230000008014 freezing Effects 0.000 abstract description 7
- 238000011084 recovery Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011109 contamination Methods 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The utility model provides a kind of cell cryopreservation tube, including pipe lid and tube body, tube body is connect with pipe lid, tube body includes outer tube and inner tube, inner tube is placed in outer tube, thermal insulation layer is equipped between inner tube and outer tube, thermal insulation layer is communicated with Material collecting box for harvesting, pipe is covered with the first bulge loop, second bulge loop and plug, plug is set in the second bulge loop, second bulge loop is set in the first bulge loop, second bulge loop is hollow structure, soft set A is equipped in second bulge loop, soft set B is equipped with outside second bulge loop, soft set A and soft set B is equipped with the second sealing ring, screw rod is equipped in second bulge loop, screw rod one end is equipped with the spring for compressing the second gasket, the other end is connect with pipe tops, interconnecting piece is equipped at the top of tube body, interconnecting piece is externally provided with concave ring, concave ring and the second bulge loop cooperate.Can tightness between reinforced pipe lid and tube body, liquid nitrogen penetrates into cryopreservation tube from pipe lid and nozzle contact position when avoiding freezing, and infects cryopreservation tube inner cell, and thermal insulation layer can reduce influence of the ambient temperature to other cryopreservation tube inner cells.
Description
Technical field
The utility model relates to the field of medical instrument technology, in particular to a kind of cell cryopreservation tube.
Background technique
Cryopreservation tube is the container that a kind of laboratory saves strain, for cell, tissue samples and strain preservation or turn
It moves.Cell cryopreservation is one of the main method that cell saves, and the cryopreservation tube equipped with cell is placed in -196 DEG C using Cryopreservation Technology
Cryo-conservation in liquid nitrogen can make cell be temporarily disengaged from growth conditions and save its cell characteristics, when needed
Recovery cell is for testing again.
Leakproofness between current existing cell cryopreservation tube pipe lid and tube body is poor, and cryopreservation tube is being placed in -196 DEG C of liquid nitrogen
During cryo-conservation, liquid nitrogen easily penetrates into cryopreservation tube, pollutes the cell frozen, and tube body and the pipe of cryopreservation tube cover
If markd label is unobvious, search difficult, prolonged search will lead in freezing storing box other and not need recovery temporarily
Cell heat absorption is melted, and multigelation can generate serious damage to the cell frozen in other cryopreservation tubes.
Summary of the invention
Mirror is with this, and the utility model proposes a kind of cell cryopreservation tube, it is simple to provide a kind of structure, flexible operation, leakproofness
Strong cryopreservation tube.
The technical solution of the utility model is achieved in that
A kind of cell cryopreservation tube, including pipe lid and tube body, the tube body are fixed on pedestal, the tube body and the pipe lid
It is threadedly coupled, the tube body includes inner and outer tubes, and said inner tube is set in the outer tube, between said inner tube and the outer tube
Equipped with thermal insulation layer, the thermal insulation layer is communicated with Material collecting box for harvesting, and the Material collecting box for harvesting is set in the pedestal, and the pipe is covered equipped with first
Bulge loop, the second bulge loop and plug, the plug are set in second bulge loop, and second bulge loop is set to first bulge loop
Interior, second bulge loop is hollow structure, and soft set A is equipped in second bulge loop, soft set is equipped with outside second bulge loop
B, the soft set A and soft set B are equipped with the second sealing ring, are equipped with screw rod in second bulge loop, one end of the screw rod is equipped with
For compressing the spring of second gasket, the other end of the screw rod is connect with the pipe tops, at the top of the tube body
Interconnecting piece equipped with protrusion, the interconnecting piece outer are equipped with concave ring, and the concave ring and second bulge loop cooperate.
Further, the pipe tops are equipped with accommodating chamber, one end of one end of the accommodating chamber bottom and the pipe lid
Rotation connection, the other end of the accommodating chamber bottom and the other end of the pipe lid snap connection.
Further, the interconnecting piece is equipped with external screw thread, and second bulge loop is equipped with internal screw thread, the pipe lid and tube body
It is connected through a screw thread.
Further, first bulge loop is equipped with internal screw thread, and the outer tube top sidewall is equipped with external screw thread, the pipe lid
It is connected through a screw thread with the tube body.
Further, the outer surface of tube body is equipped with antiskid thread.
Further, the top cylinder type structure of said inner tube, the lower part of said inner tube are pyramid type structure.
Compared with prior art, the utility model has the beneficial effects that
The pipe of cryopreservation tube is covered equipped with the first bulge loop, and the first bulge loop is threadedly coupled with tube body, is played certain sealing and is made
With preventing liquid nitrogen from entering in cryopreservation tube, interconnecting piece be equipped at the top of tube body, the plug being located in the second bulge loop can seal interconnecting piece
Opening further strengthens the sealing effect between pipe lid and tube body, and concave ring and the second bulge loop cooperate, and the second bulge loop is equipped with soft
Set A and soft set B plays the role of sealing to the hollow structure of the second bulge loop, prevents liquid nitrogen or other pollutants from the second bulge loop
Hollow structure enters in cryopreservation tube, at the same can also in the pushing of spring bullet change the height of soft set A and soft set B, wherein
Spring can prevent from excessively twisting screw rod and leading to the case where freezing tracheal rupture, the second sealing ring can be made to concave ring by turning screw rod
It compresses, liquid nitrogen is avoided to enter in cryopreservation tube, cause the pollution of freeze-stored cell, thermal insulation layer can reduce temperature to freezing in other cryopreservation tubes
The influence for depositing cell, avoids multigelation from causing to damage to cell, and thermal insulation layer is communicated with Material collecting box for harvesting, can recover to cell
When, the heat-barrier material in thermal insulation layer is collected into Material collecting box for harvesting, in order to avoid influencing the effect of cell recovery, structure is simple, operation
Flexibly.
Detailed description of the invention
Fig. 1 is a kind of the schematic diagram of the section structure of cell cryopreservation tube of the utility model；
Fig. 2 is a kind of partial structurtes enlarged diagram of cell cryopreservation tube of the utility model.
In figure, 1 plug, 2 screw rods, 3 first sealing rings, 4 first bulge loops, 5 thermal insulation layers, 6 inner tubes, 7 outer tubes, 8 pedestals, 9 are prevented
Sliding screw thread, 10 concave rings, 11 interconnecting pieces, 12 second bulge loops, 13 accommodating chambers, 14 second sealing rings, 15 springs, 16 soft set A, outside 17
Ring, 18 soft set B, 19 inner ring, 20 Material collecting box for harvesting, 21 tube bodies, 22 pipe lids.
Specific embodiment
In order to be best understood from the utility model technology contents, specific embodiment is provided below, and practical to this in conjunction with attached drawing
It is novel to be described further.
Embodiment
Referring to Fig. 1 to Fig. 2, a kind of cell cryopreservation tube provided by the utility model, including pipe lid 22 and tube body 21, the pipe
Body 21 is fixed on pedestal 8, indicates scale on tube body 21, is easy to understand the capacity of freeze-stored cell, the tube body 21 and the pipe
Lid 22 be threadedly coupled, the tube body 21 include inner tube 6 and outer tube 7, said inner tube 6 be set to the outer tube 7 in, said inner tube 6 with
Thermal insulation layer 4 is equipped between the outer tube 7, the thermal insulation layer 4 communicates with Material collecting box for harvesting 20, and wherein be provided with can be heat-insulated for thermal insulation layer 4
Material, between thermal insulation layer 4 and Material collecting box for harvesting 20 be equipped with valve (not shown), for control between thermal insulation layer 4 and Material collecting box for harvesting 20 every
Hot material, wherein thermal insulation layer 4 is communicated with Material collecting box for harvesting 20, can be when freeze-stored cell is carried out recovery, by the heat-insulated material in thermal insulation layer 4
Material is collected in Material collecting box for harvesting 20, in order to avoid influencing to carry out freeze-stored cell the effect of heating water bath when recovery, the Material collecting box for harvesting 20 is set
In in the pedestal 8, saving space, the pipe lid 22 is equipped with the first bulge loop 4, the second bulge loop 12 and plug 1, the plug 1
In second bulge loop 12, second bulge loop 12 is set in first bulge loop 4, the top of the first bulge loop 4 and outer tube 7
Sidewall thread connection, guarantee pipe lid 22 and tube body 21 leakproofness, second bulge loop 12 be hollow structure, described second is convex
The inner ring 19 of ring 12 is equipped with soft set A16, and the outer ring 17 of second bulge loop 12 is equipped with soft set B18, the soft set A16 and soft set
B18 is equipped with the second sealing ring 14, and screw rod 2 is equipped in second bulge loop 12, and one end of the screw rod 2 is equipped with for compressing
The spring 15 of the second gasket is stated, connects at the top of the other end of the screw rod 2 and the pipe lid 22, is equipped at the top of the tube body 21
The interconnecting piece 11 of protrusion, wherein plug 1 can be used for clogging the opening of interconnecting piece 11, further strengthen between pipe lid 22 and tube body 21
Leakproofness, 11 outer of interconnecting piece is equipped with concave ring 10, be equipped with the first sealing ring 3 in concave ring 10, the concave ring 10 with it is described
The cooperation of second bulge loop 12, can be compressed the second sealing ring 14 to the first sealing ring 3 by turning screw rod 2, avoided leaking into and
Liquid nitrogen enters in cryopreservation tube, and wherein spring 15 can play the role of buffering, when preventing from excessively firmly turning screw rod 2 downwards, will manage
Body 21 crush, wherein soft set A16 and soft set B18 can in the pushing of spring 15 bullet change the height of soft set A16 and soft set B18
Degree.When using the cryopreservation tube, first the heat-barrier material in Material collecting box for harvesting 20 is poured into thermal insulation layer 4, secondly freezes needs
Cell is packed into cryopreservation tube, and finally pipe lid 22 is covered on tube body 21, turns screw rod 2, makes the first sealing ring 3 and the second sealing ring
14 compress, and the cryopreservation tube being sealed is put into liquid nitrogen and is frozen.
Specifically, accommodating chamber 13 is equipped at the top of the pipe lid 22, one end of 13 bottom of accommodating chamber and the pipe lid 22
One end rotation connection, the other end of the other end and the pipe lid 22 of 13 bottom of accommodating chamber snaps connection, accommodating chamber 13
It can be used for being packed into the badge card of cellular informatics, label is placed in top can be easy-to-look-up, rotates at the top of accommodating chamber 13 and pipe lid 22
Connection is conducive to the operation of screw rod 2, when needing to compress or loosening the second sealing ring 14, can lead to 13 rotating opening of accommodating chamber
It crosses and turns screw rod 2, unclamp or compress the second sealing ring 14, outer tube 7 is equipped with mark zone (not shown), by pipe lid 22 and tube body
21 separate when, conveniently find matched pipe lid 22 and tube body 21, pipe lid 22 avoided to obscure with tube body 21, misused other freeze
The pipe lid 22 for depositing pipe, causes cell to be infected.
Specifically, the interconnecting piece 11 is equipped with external screw thread (not shown), second bulge loop 12 is equipped with internal screw thread and (does not mark
Out), the pipe lid 22 is connected through a screw thread with tube body 21, the compactness between reinforced pipe lid 22 and tube body 21, prevent liquid nitrogen or
Other pollution sources, which enter, causes cell contamination in cryopreservation tube.
Specifically, first bulge loop 4 is equipped with internal screw thread (not shown), 7 top sidewall of outer tube is equipped with external screw thread
(not shown), the pipe lid 22 are connected through a screw thread with the tube body 21, the compactness between reinforced pipe lid 22 and tube body 21, are prevented
Only liquid nitrogen or other pollution sources, which enter, causes cell contamination in cryopreservation tube.
Specifically, 21 outer surface of tube body, which is equipped with antiskid thread 9, can reinforce hand and Guan Gai when unscrewing pipe lid 22
Frictional force between 22 facilitates and unscrews pipe lid 22.
Specifically, the top cylinder type structure of said inner tube 6, the lower part of said inner tube 6 is pyramid type structure, tube body 21
Top is that cylinder type can be increased the capacity in pipe, can also match the structure of the fixation cryopreservation tube of existing freezing storing box, cell
It is easy precipitating when freezing to be adhered to and freezes bottom of the tube, causing to be adhered to the cell for freezing bottom of the tube can not reuse, under inner tube 6
Portion is that cone can reduce freeze-stored cell precipitating adhered area, improves the availability of freeze-stored cell.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Within the spirit and principle of utility model, any modification, equivalent replacement, improvement and so on should be included in the utility model
Protection scope within.
Claims (6)
1. a kind of cell cryopreservation tube, it is characterised in that: including pipe lid and tube body, the tube body is fixed on pedestal, the tube body
It is threadedly coupled with the pipe lid, the tube body includes inner and outer tubes, and said inner tube is set in the outer tube, said inner tube and institute
It states and is equipped with thermal insulation layer between outer tube, the thermal insulation layer is communicated with Material collecting box for harvesting, and the Material collecting box for harvesting is set in the pedestal, the pipe lid
It is equipped with the first bulge loop, the second bulge loop and plug, the plug is set in second bulge loop, and second bulge loop is set to described
In first bulge loop, second bulge loop is hollow structure, is equipped with soft set A in second bulge loop, second bulge loop
It is equipped with soft set B, the soft set A and soft set B outside equipped with the second sealing ring, second bulge loop is interior to be equipped with screw rod, the spiral shell
One end of bar is equipped with the spring for compressing second gasket, and the other end of the screw rod is connect with the pipe tops,
The interconnecting piece of protrusion is equipped at the top of the tube body, the interconnecting piece outer is equipped with concave ring, and the concave ring is matched with second bulge loop
It closes.
2. a kind of cell cryopreservation tube as described in claim 1, it is characterised in that: the pipe tops are equipped with accommodating chamber, described
One end of accommodating chamber bottom and one end of the pipe lid are rotatablely connected, and the other end of the accommodating chamber bottom is another with the pipe lid
One end snaps connection.
3. a kind of cell cryopreservation tube as described in claim 1, it is characterised in that: the interconnecting piece is equipped with external screw thread, and described the
Two bulge loops are equipped with internal screw thread, and the pipe lid is connected through a screw thread with tube body.
4. a kind of cell cryopreservation tube as described in claim 1, it is characterised in that: first bulge loop is equipped with internal screw thread, institute
Outer tube top sidewall is stated equipped with external screw thread, the pipe lid is connected through a screw thread with the tube body.
5. a kind of cell cryopreservation tube as described in claim 1, it is characterised in that: the outer surface of tube body is equipped with anti-skidding spiral shell
Line.
6. a kind of cell cryopreservation tube as described in claim 1, it is characterised in that: the top cylinder type structure of said inner tube, institute
The lower part for stating inner tube is pyramid type structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822209879.7U CN208768813U (en) | 2018-12-27 | 2018-12-27 | A kind of cell cryopreservation tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822209879.7U CN208768813U (en) | 2018-12-27 | 2018-12-27 | A kind of cell cryopreservation tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208768813U true CN208768813U (en) | 2019-04-23 |
Family
ID=66163794
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822209879.7U Expired - Fee Related CN208768813U (en) | 2018-12-27 | 2018-12-27 | A kind of cell cryopreservation tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208768813U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115428787A (en) * | 2022-09-29 | 2022-12-06 | 邵阳学院 | Cryopreservation device for researching cell culture based on molecular biological DNA |
-
2018
- 2018-12-27 CN CN201822209879.7U patent/CN208768813U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115428787A (en) * | 2022-09-29 | 2022-12-06 | 邵阳学院 | Cryopreservation device for researching cell culture based on molecular biological DNA |
| CN115428787B (en) * | 2022-09-29 | 2023-10-13 | 邵阳学院 | Freezing device for researching cell culture based on molecular biological DNA |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190423 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |