CN216088520U - Self-exhausting specimen freezing tube - Google Patents

Abstract

The utility model relates to a self-exhaust specimen cryopreservation tube which comprises a tube body and a tube cover, wherein the tube cover is covered on an opening at the top of the tube body, the inner wall of the tube body is connected with an exhaust piece through a plurality of connecting ribs, and the exhaust piece extends into the bottom in the tube body. The utility model can realize the rapid filling of the whole tissue and cell suspension, the bottom gas can be rapidly discharged from the exhaust part, the space occupying effect is not formed, the operation process is greatly simplified, on one hand, the complicated steps of cutting and crushing the specimen for subpackage can be reduced, and the integrity of the specimen is kept; on the other hand, for the frozen cell suspension, a pipette gun can be used for quickly pumping out the cell suspension with the corresponding volume, and the condition that the total volume is influenced because the gas at the bottom of the tube is not discharged in time can not occur.

Description

Self-exhausting specimen freezing tube

Technical Field

The utility model relates to the technical field of biomedicine, in particular to a self-exhausting specimen cryopreservation tube which is suitable for rapid tube loading and maintaining of fresh in-vitro specimens in surgical operations.

Background

The establishment of a tissue sample specimen bank is more and more indispensable in the construction of research hospitals, in the process of preserving in vitro fresh specimens in surgical operations, the specimens are required to be completely filled in a single freezing tube as soon as possible and placed in liquid nitrogen for preservation, and once the tissues leave a living body, the tissues are preserved in vitro for scientific research use, and various biological characteristics of the tissues are gradually changed. Therefore, it is very important to preserve the tissue specimen in time.

Liquid nitrogen cryopreservation is one of the main methods for sample preservation. The sample is placed in liquid nitrogen at the temperature of-196 ℃ for low-temperature preservation by utilizing a freezing preservation technology, the characteristics of the tissue sample can be temporarily preserved, the tissue sample can be taken out for treatment for an experiment when needed, a certain amount of samples can be preserved as much as possible, and the case loss caused by the pollution or other accidents of the sample in the experiment can be prevented, so that the effect of long-term preservation and seed preservation of the sample library is achieved; in addition, the frozen sample storage mode can be used for sending, exchanging, sharing and transporting samples. In order to achieve the purpose, the key is the quick and effective material taking and freezing storage technology. Under normal conditions, besides adding a protective agent to reduce the freezing point during tissue cryopreservation, the cryopreservation technology is characterized in that the time for loading the sample into the tube is shortened, the whole tissue is placed in a single tube as much as possible so as to be beneficial to later-stage experimental material taking, and the time for taking the material to the cryopreservation is shortened, namely the time for shortening the cell at normal temperature is critical, and the tissue sample can be quickly placed into the cryopreservation tube in a whole block under an aseptic state is particularly important.

Most of the currently applied tissue cryopreservation tubes are of uniform diameter specifications with the inner diameter of 0.50inch and the tube cover size of 0.54inch, and main manufacturers have brands such as corning and nalgene. Clinical specimen collection uses corn more, and it is though 1ml to 4ml unequal, and the internal diameter does not have the change, and the volume increases mainly to rely on length to increase, owing to freeze and deposit the box, the specification of attached consumptive material such as liquid nitrogen container cryopreserved frame is comparatively unified, except that under few utmost special circumstances, all adopt 2ml corn to freeze at home and abroad and deposit the pipe and carry out the sample collection.

When collecting the sample, use neurosurgery brain glioma tissue as an example, general sample divide into two pipes, a pipe is peripheral tumor tissue, a pipe is tumour central tissue, glioma tissue matter is soft, it is breakable, if the fritter is cut, the later stage is carried out the section and is dyed the difficulty of drawing materials, also be difficult to distinguish the structure simultaneously, it cuts to be bar or monoblock on the separation tumor sample more to draw materials, when putting into intraductal, often because of the tissue touches the wall compression, the pipe diameter is less and leads to intraductal gas extrusion at the bottom of a tube (use 2ml pipe as an example, when placing the sample fast, the air of extrusion at the bottom of a tube can cause 1 ml's volume waste sometimes), the cryopreserved pipe can't be filled up to the tissue, need take out repeatedly slowly to place again, let intraductal gas have the seam to extrude.

This problem also often has when the cell freezes deposits, if use the pipetting gun to beat out the cell suspension too fast, also will lead to intraductal gas in time to discharge and closed at the bottom of the tube, extravagant space, need absorb repeatedly, need stretch into intraductal with the pipetting gun rifle head sometimes, for the operation brings the trouble, also probably be stained with the cell suspension outside the rifle head, lead to the cell extravagant. The working efficiency is reduced to a certain extent, and the operation time is prolonged, so that the cell freezing quality is influenced; even because a large amount of cells need to be frozen at a time, the gas backlog at the bottom of the tube is not noticed during operation, and the cells are continuously pumped into the cell suspension to cause overflow, thereby causing great loss to the experiment.

If the gas is left in the tube, when the tissue sample is taken for cryopreservation and resuscitation, the gas compressed by cooling with liquid nitrogen is checked and searched when the sample is thawed and expands with heat, which can directly cause the tube cover to be flushed, thus causing the sample or cells to be sprayed, being polluted and wasted and causing great loss.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problems in the prior art. Therefore, the utility model provides a self-exhausting specimen cryopreservation tube.

The technical scheme adopted by the utility model for solving the technical problems is as follows:

the utility model provides a from sample cryopreserving pipe of exhausting, locate including body and lid body open-top's tube cap, the inner wall of body is connected with the air discharge member through a plurality of splice bars, the air discharge member stretches into bottom in the body.

In a preferred embodiment of the self-venting specimen cryopreservation tube provided by the utility model, the venting member is an exhaust tube, and the top of the venting member does not extend out of the tube body.

In a preferred embodiment of the self-venting specimen cryopreservation tube provided by the utility model, the venting member comprises a connecting rod and a venting tube, the connecting rod is arranged outside the venting tube and is integrally arranged with the venting tube along the length direction of the venting tube, and the connecting rod is connected with the inner wall of the tube body through a plurality of connecting ribs.

In a preferred embodiment of the self-degassing specimen cryopreservation tube provided by the utility model, the top of the connecting rod extends out of the tube body, and the length of the connecting rod extending out of the tube body is less than the depth of the tube cover.

In a preferred embodiment of the self-venting specimen cryopreservation tube provided by the utility model, the bottom of the vent tube is provided with a chamfered opening structure, and the opening faces to the inner wall side of the tube body close to the vent tube.

In a preferred embodiment of the self-venting specimen cryopreservation tube provided by the utility model, the venting member and the connecting rib are made of the same material as the tube body.

Compared with the prior art, the self-exhaust specimen cryopreservation tube provided by the utility model has the beneficial effects that: the utility model can realize the rapid filling of the whole tissue and cell suspension, the bottom gas can be rapidly discharged from the exhaust part, the space occupying effect is not formed, the operation process is greatly simplified, on one hand, the complicated steps of cutting and crushing the specimen for subpackage can be reduced, and the integrity of the specimen is kept; on the other hand, for the frozen cell suspension, a pipette gun can be used for quickly pumping out the cell suspension with the corresponding volume, and the condition that the total volume is influenced because the gas at the bottom of the tube is not discharged in time can not occur. The operation is greatly simplified, the freezing time is shortened, and the freezing quality and the working efficiency are improved. The exhaust member is connected in the body by the splice bar, can be directly after accomplishing the sample and putting, and the exhaust member is taken out completely, and later the cryopreserving pipe can directly be used for the centrifugation.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:

FIG. 1 is a cross-sectional view of a preferred embodiment of a self-venting specimen vial provided in accordance with the present invention;

FIG. 2 is a cross-sectional view A-A of FIG. 1;

FIG. 3 is a cross-sectional view of another preferred embodiment of a self-venting sample vial provided in accordance with the present invention;

FIG. 4 is a cross-sectional view A-A provided in FIG. 3;

FIG. 5 is a view showing a state in which the air discharging member is taken out after the specimen is placed in the self-air discharging specimen cryopreservation tube according to the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance; furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, as they may be fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example one

The embodiment provides a self-venting specimen cryopreservation tube 1, as shown in fig. 1 to 4, comprising a tube body 101 and a tube cap 102 covering the top opening of the tube body, in the embodiment, the tube body and the tube cap are screwed, in other embodiments, the tube body and the tube cap can also adopt other covering modes, and the specific covering mode is not limited in the application and can be determined according to the design requirements of the cryopreservation tube.

In order to solve the problem that the intraductal gas that the quick filling of current cryopreserved pipe at monoblock tissue, cell suspension exists can't in time get rid of, this embodiment is in the inner wall of body 101 is connected with exhaust piece 104 through a plurality of splice bar 103, exhaust piece 104 stretches into bottom in the body, as shown in figure 1 and figure 2, this embodiment design exhaust piece can in time be with the gaseous discharge of body bottom when filling monoblock tissue or cell suspension fast, exhaust piece is connected in the body by the splice bar, and the splice bar design is the piece of easily breaking down, can directly accomplish the sample and put the back, completely take out exhaust piece, as shown in figure 5, later cryopreserved pipe can directly be used for the centrifugation.

Example two

On the basis of the first embodiment, the exhaust member 104 of the present embodiment is an exhaust pipe, and the top of the exhaust pipe does not extend out of the pipe body 101, as shown in fig. 1 and fig. 2, so that the normal covering of the pipe body and the pipe cover is not affected.

EXAMPLE III

Different from the second embodiment, the exhaust member 104 of this embodiment includes a connecting rod 1041 and an exhaust pipe 1042, as shown in fig. 3 and fig. 4, the connecting rod 1041 is disposed outside the exhaust pipe 1042, the connecting rod 1041 is integrally disposed with the exhaust pipe 1042 along the length direction of the exhaust pipe 1042, and the connecting rod 1041 is connected to the inner wall of the pipe body 101 through a plurality of connecting ribs 103. This embodiment the design of connective bar breaks the atress piece of splice bar off with the fingers and thumb as breaking off with the fingers and thumb, conveniently accomplishes the sample and places the back, completely takes out the exhaust spare.

Preferably, the top of the connecting rod 1041 of this embodiment extends out of the tube 101, and the length L1 extending out of the tube is less than the depth L2 of the tube cover, so that the connecting rod does not affect the connection between the tube cover and the tube.

Example four

Based on any of the above embodiments, the bottom of the exhaust pipe of this embodiment is designed as a chamfered opening structure, and the opening faces the inner wall side of the pipe body near the chamfered opening structure, as shown in fig. 1 and fig. 3.

In the above embodiment, the air discharging member 104 and the connecting rib 103 are made of the same material as the pipe 101.

It is worth noting that: the freezing pipe comprises any shape of the existing freezing pipe, and the bottom of the pipe body can be designed into an arc shape, a flat bottom shape or any other existing shape.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (6)

1. The utility model provides a from exhaust sample cryopreserved pipe, includes that body and lid are located body open-top's tube cap, its characterized in that: the inner wall of the pipe body is connected with an exhaust piece through a plurality of connecting ribs, and the exhaust piece extends into the bottom in the pipe body.

2. The self-venting specimen cryopreservation tube of claim 1, wherein: the exhaust member is an exhaust pipe, and the top of the exhaust member does not extend out of the pipe body.

3. The self-venting specimen cryopreservation tube of claim 1, wherein: the exhaust piece includes connective bar and blast pipe, the connective bar is located the blast pipe outside, just the connective bar is followed the length direction of blast pipe with the blast pipe is integrative to be set up, the connective bar is connected through a plurality of splice bars the inner wall of body.

4. The self-venting specimen cryopreservation tube of claim 3, wherein: the top of the connecting rod extends out of the pipe body, and the length of the connecting rod extending out of the pipe body is smaller than the depth of the pipe cover.

5. The self-venting specimen cryopreservation tube of any one of claims 2 to 4, wherein: the bottom of the exhaust pipe is of a chamfered surface opening structure, and the opening faces the inner wall side of the pipe body close to the exhaust pipe.

6. The self-venting specimen cryopreservation tube of claim 1, wherein: the exhaust member and the connecting ribs are made of the same material as the pipe body.

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