CN215270258U

CN215270258U - Liquid nitrogen container is stored to stem cell

Info

Publication number: CN215270258U
Application number: CN202121068443.6U
Authority: CN
Inventors: 张璇; 李媛媛; 姚海霞; 杨林霏; 黄雷震
Original assignee: Jinan Saier Biological Technology Co ltd
Current assignee: Jinan Saier Biological Technology Co ltd
Priority date: 2021-05-18
Filing date: 2021-05-18
Publication date: 2021-12-24
Anticipated expiration: 2031-05-18

Abstract

The application discloses liquid nitrogen container is stored to stem cell belongs to cell storage facilities technical field. This stem cell stores liquid nitrogen container includes: the liquid nitrogen tank comprises a liquid nitrogen tank body, wherein a sealing opening is formed in the top of the liquid nitrogen tank body, the sealing opening comprises a first sealing opening section and a second sealing opening section, the first sealing opening section and the second sealing opening section are coaxially arranged, the first sealing opening is positioned below the second sealing opening, and the inner side wall of the first sealing opening section extends towards the axial direction of the sealing opening relative to the inner side wall of the second sealing opening section to form an annular boss, so that the radial size of the first sealing opening section is smaller than that of the second sealing opening section; and the sealing cover at least can seal the second sealing opening section. The liquid nitrogen tank for storing the stem cells reduces the exchange between the tank body of the liquid nitrogen tank and external energy, reduces the reduction of the liquid level in the tank body of the liquid nitrogen tank, reduces the influence on the activity of the stem cells due to the change of the liquid level, and improves the safety of the whole liquid nitrogen tank for storing the stem cells.

Description

Liquid nitrogen container is stored to stem cell

Technical Field

The application relates to a liquid nitrogen tank is stored to stem cell belongs to cell storage facilities technical field.

Background

With the development of medical level, the preservation of biological samples and life genetic resources becomes important research foundation and resources in biology and medicine, and the liquid nitrogen low-temperature preservation of stem cells attracts more and more attention.

The long-term cryopreservation of stem cells for clinical and scientific purposes is generally realized by using liquid nitrogen or liquid nitrogen steam which can reach-196 ℃. The stem cells are stored at a deep low temperature of-196 ℃, the metabolic activity of the stem cells stops almost at the deep low temperature, the tissue cells are rarely damaged under the correct storage operation, and the survival rate of the cells stored for a long time is more than 80 percent. In order to avoid the damage to cells caused by the formation of ice crystals and cell deformation of water inside and outside the cells due to sudden temperature drop, a material to be preserved is firstly placed in a freezing protection solution, slowly frozen or vitrified for temperature reduction through a program, and then is put into liquid nitrogen for long-term preservation. The cryopreservation method is suitable for stem cells, and is also suitable for storing various cells, strains, tissue blocks and the like.

The existing liquid nitrogen tank for storing stem cells is characterized in that a sealing opening and a sealing cover of the existing liquid nitrogen tank are of cylindrical or round table-shaped structures, and due to the existence of errors, a gap is inevitably formed between the sealing opening and the sealing cover, so that the sealing performance is poor, the energy exchange between a tank body of the liquid nitrogen tank and the outside is caused, the reduction of the liquid level in the liquid nitrogen tank is accelerated, if liquid nitrogen cannot be supplemented in time, the activity of stem cells can be influenced, and finally the safety of the whole liquid nitrogen tank for storing stem cells is relatively low. Therefore, the existing liquid nitrogen tank for storing the stem cells needs to be improved so as to solve the problem that the existing liquid nitrogen tank for storing the stem cells is poor in sealing performance.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems, the application provides a stem cell storage liquid nitrogen tank. The technical scheme of the application is as follows:

the application provides a liquid nitrogen container is stored to stem cell includes:

the top of the liquid nitrogen tank body is provided with a sealing opening, the sealing opening comprises a first sealing opening section and a second sealing opening section, the first sealing opening section and the second sealing opening section are coaxially arranged, the first sealing opening is positioned below the second sealing opening, and the inner side wall of the first sealing opening section extends towards the axial direction of the sealing opening relative to the inner side wall of the second sealing opening section to form an annular boss, so that the radial dimension of the first sealing opening section is smaller than that of the second sealing opening section;

a seal cap capable of sealing at least the second seal port segment.

Optionally, the sealing cover is further provided with an extension section extending towards the first sealing opening section, and the extension section is used for sealing the first sealing opening section.

Optionally, still include stem cell bag and carry the utensil, stem cell bag carries the utensil and includes the shadoof and establish the carrier in shadoof one end, sealed mouth is used for stem cell bag carries the utensil stretch into to the liquid nitrogen tank jar is internal, annular boss is equipped with prevents the steadiness piece of shadoof motion.

Optionally, the steadiness piece is including seting up the first joint groove of annular boss inside wall, first joint groove is followed the one end of annular boss extends to its other end, and first joint groove edge the axis direction setting of sealing port, first joint groove is used for the joint the shadoof.

Optionally, the lifting rod comprises a first lifting rod section, an over-connection section and a second lifting rod section which are connected in sequence, and the first lifting rod section and the second lifting rod section are arranged in parallel;

the fixing piece further comprises a second clamping groove which is formed in the end portion, close to the second sealing opening section, of the annular boss, the second clamping groove is used for clamping the transition connecting section, and the first clamping groove is used for clamping the first lifting rod section;

and a third clamping groove is formed in the side wall of the second sealing opening section and/or the side wall of the sealing cover and used for clamping the second lifting rod section.

Optionally, a limiting piece is arranged at one end, away from the carrier, of the lifting rod, and the limiting piece is used for preventing the lifting rod from sliding into the liquid nitrogen tank body;

one end of the sealing cover, which is close to the annular boss, is provided with a containing groove for containing the limiting part.

Optionally, the carrier is a cylindrical container, and an opening is formed in a lower portion of a bottom wall and/or a side wall of the cylindrical container.

Optionally, a temperature sensor and a liquid level sensor are arranged in the liquid nitrogen tank body, and when the stem cells are in a storage state, the liquid level sensor is not lower than the carrier.

Optionally, the liquid nitrogen storage tank further comprises a display arranged on the outer side wall of the liquid nitrogen storage tank body, and the display is electrically connected with the temperature sensor and the liquid level sensor.

Optionally, a hidden handle is provided on the top of the sealing cover.

Benefits that can be produced by the present application include, but are not limited to:

1. the utility model provides a liquid nitrogen tank is stored to stem cell, the annular boss that the first seal mouthful section of its seal port set up, make the lateral wall of sealed lid and the inside wall of second seal mouthful section meet the department mutually, the diapire of sealed lid contacts with the roof of annular boss, the contact of sealed lid and seal mouthful lateral wall is not on the coplanar with the contact of diapire, greatly reduced the exchange of liquid nitrogen tank jar body and external energy, the reduction of the internal liquid level of liquid nitrogen tank has been reduced, the influence to stem cell activity because of the change of liquid level has been reduced, whole stem cell storage liquid nitrogen tank's security has been improved.

2. According to the stem cell storage liquid nitrogen tank provided by the application, the arrangement of the extension section of the sealing cover can further improve the sealing property of the whole stem cell storage liquid nitrogen tank; the arrangement of the fixing piece on the annular boss can prevent the lifting rod from rotating, and reduce the influence of the stem cell storage liquid nitrogen tank on the activity of stem cells in the transportation process; the arrangement of the first lifting rod section, the transition connecting section and the second lifting rod section of the lifting rod can further improve the stability of the lifting rod, and can reduce the frostbite of liquid nitrogen atomization on the hands of an operator in the process of taking out stem cells; the carrier is arranged in a cylindrical container, and the lower part of the bottom wall or the side wall of the carrier is provided with an opening, so that liquid nitrogen can quickly enter the cylindrical container and gradually immerse the stem cell bag; the temperature sensor and the liquid level sensor are arranged to monitor the temperature and the liquid level in the liquid nitrogen tank body in real time and transmit the temperature and the liquid level to the display so as to supplement liquid nitrogen in the liquid nitrogen tank body in time.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:

FIG. 1 is a schematic structural diagram of a liquid nitrogen tank for storing stem cells according to an embodiment of the present disclosure;

FIG. 2 is a sectional view taken along line A-A of FIG. 1;

FIG. 3 is a schematic diagram of another liquid nitrogen tank for storing stem cells;

FIG. 4 is a sectional view taken along line B-B of FIG. 3;

FIG. 5 is a schematic representation of a stem cell vector.

List of parts and reference numerals:

1. a tank body of a liquid nitrogen tank,

2. sealing opening, 21, first sealing opening section, 22, second sealing opening section, 221, third clamping groove, 23, annular boss, 231, first clamping groove, 232 and second clamping groove

3. A sealing cover 31, a containing groove 32, an extending section,

4. a lifting rod 41, a first lifting rod section 42, a transition connecting section 43, a second lifting rod section 44, a limiting piece 45 and a hook,

5. a carrier (51) for holding the substrate in a predetermined position,

6. temperature sensor, 7, liquid level sensor, 8, display, 9, hidden handle.

Detailed Description

In order to more clearly explain the overall concept of the present application, the following detailed description is given by way of example in conjunction with the accompanying drawings.

In order that the above objects, features and advantages of the present application can be more clearly understood, the present application will be described in further detail with reference to the accompanying drawings and detailed description. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, however, the present application may be practiced in other ways than those described herein, and therefore the scope of the present application is not limited by the specific embodiments disclosed below.

The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

The embodiment of the application discloses liquid nitrogen storage tank for stem cells, as shown in fig. 1 and 3, including liquid nitrogen storage tank body 1 and sealed lid 3, sealed mouth 2 has been seted up at the top of liquid nitrogen storage tank body 1, sealed mouth 2 includes first sealed mouthful section 21 and second sealed mouthful section 22, first sealed mouthful section 21 is located the below of second sealed mouthful section 22, first sealed mouthful section 21 and second sealed mouthful section 22 coaxial line set up, the inside wall of first sealed mouthful section 21 extends to the axis of sealed mouthful 2 relative second sealed mouthful section 22 inside wall to form annular boss 23, so that the radial dimension of first sealed mouthful section 21 is less than the radial dimension of second sealed mouthful section 22, this sealed lid 3 can seal second sealed mouthful section 22.

In order to further improve the sealing performance of the liquid nitrogen tank for storing stem cells, the sealing cover 3 is further provided with an extension section 32 extending towards the first sealing opening section 21, and the extension section 32 can seal the first sealing opening section 21, namely, the side wall of the extension section 32 is in contact with the side wall of the first sealing opening section 21.

When stem cell storage liquid nitrogen container is in encapsulated situation, the lateral wall of sealed lid 3 meets the department with the inside wall of second seal mouthful section 22, the diapire of sealed lid 3 contacts with the roof of annular boss 23, the contact of sealed lid 3 and 2 lateral walls of seal mouthful is not on the coplanar with the contact of diapire, greatly reduced the exchange of the liquid nitrogen container body 1 with external energy, reduced the reduction of the internal liquid level of liquid nitrogen container body 1, reduced because of the change of liquid level to the active influence of stem cell, whole stem cell storage liquid nitrogen container's security has been improved.

In one embodiment, as shown in fig. 1 and 2, the liquid nitrogen tank for storing stem cells further comprises a stem cell bag lifting tool, the stem cell bag lifting tool comprises a lifting rod 4 and a carrier 5 arranged at one end of the lifting rod 4, the sealing port 2 can allow a stem cell carrier to extend into the liquid nitrogen tank body 1, and the annular boss 23 is provided with a fixing piece for preventing the lifting rod 4 from moving.

As an implementation mode, the fixing member includes a first clamping groove 231 formed in the inner side wall of the annular boss 23, the first clamping groove 231 extends from one end of the annular boss 23 to the other end of the annular boss, the first clamping groove 231 is arranged along the central axis direction of the sealing port 2, and the lifting rod 4 can be clamped in the first clamping groove 231, so that the lifting rod 4 is prevented from rotating in the transportation process and affecting the activity of stem cells; in order to prevent the lifting rod 4 from sliding down to the liquid nitrogen tank body 1 along the first clamping groove 231, the top of the lifting rod 4 is provided with a limiting part 44, the specific limiting part 4 is a cap body structure, in order to further improve the sealing performance of the liquid nitrogen tank for storing stem cells, one end of the sealing cover 3 close to the annular boss 23 is provided with a holding groove 31 for holding the limiting part of the cap body structure, and the structure of the holding groove 31 is the same as the shape of the limiting part 44, so that the sealing cover 3 is in close contact with the annular boss 23 and the limiting part 44.

In another embodiment, as shown in fig. 3, 4 and 5, the lifting bar 4 comprises a first lifting bar section 41, an intermediate connecting section 42 and a second lifting bar section 43 in sequence, wherein the first lifting bar section 41 and the second lifting bar section 43 are arranged in parallel and are not on the same straight line; the fixing piece further comprises a second clamping groove 232 formed in the top of the annular boss 23, the transition connecting section 42 can be clamped in the second clamping groove 232, and the first lifting rod section 41 can be clamped in the first clamping groove 231; the side wall of the second seal opening section 22 or the side wall of the seal cover 3 is provided with a third clamping groove 221, and the second lifting rod section 43 can be clamped in the third clamping groove 221. Further, a hook 45 is arranged at the top of the second lifting rod section 43, so that the use of an operator is facilitated, and the frostbite of the hand of the operator due to liquid nitrogen atomization is reduced.

In the transportation process of the liquid nitrogen tank for storing the stem cells, in order to prevent the stem cell bag from being separated from the fixing hook of the lifting rod 4, the bottom of the lifting rod 4 is provided with a carrier 5, as an implementation mode, the carrier 5 is a cylindrical container, the lower part of the bottom wall and/or the side wall of the cylindrical container is provided with an opening 51, the stem cell bag can be immersed gradually in the cylindrical container by enabling the liquid nitrogen to enter the cylindrical container more quickly, and the damage caused by the impact of the liquid nitrogen on the stem cells is reduced.

In order to monitor the temperature and the liquid level in the liquid nitrogen tank body 1 in real time and supplement the liquid nitrogen in the stem cell storage liquid nitrogen tank in time, a temperature sensor 6 and a liquid level sensor 7 are arranged in the liquid nitrogen tank body 1, and a display 8 electrically connected with the temperature sensor 6 and the liquid level sensor 7 is arranged on the outer side wall of the liquid nitrogen tank body 1.

In one embodiment, the top of the sealing lid 3 is provided with a hidden handle 9 to facilitate the mounting and dismounting of the sealing lid 3.

The embodiments in the present specification are described in a progressive manner, and the same and similar parts among the embodiments are referred to each other, and each embodiment focuses on the differences from the other embodiments. In particular, for the system embodiment, since it is substantially similar to the method embodiment, the description is simple, and for the relevant points, reference may be made to the partial description of the method embodiment.

The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims

1. A liquid nitrogen tank for storing stem cells, comprising:

the liquid nitrogen tank body (1) is provided with a sealing opening (2) at the top of the liquid nitrogen tank body (1), the sealing opening (2) comprises a first sealing opening section (21) and a second sealing opening section (22), the first sealing opening section (21) and the second sealing opening section (22) are coaxially arranged, the first sealing opening section (21) is positioned below the second sealing opening section (22), and the inner side wall of the first sealing opening section (21) extends towards the axial direction of the sealing opening (2) relative to the inner side wall of the second sealing opening section (22) to form an annular boss (23), so that the radial dimension of the first sealing opening section (21) is smaller than that of the second sealing opening section (22);

a sealing cover (3), the sealing cover (3) being capable of sealing at least the second sealing opening section (22).

2. The liquid nitrogen tank for stem cell storage according to claim 1, wherein the sealing cover (3) is further provided with an extension (32) extending toward the first sealing port section (21), the extension (32) being for sealing the first sealing port section (21).

3. The liquid nitrogen tank for stem cell storage according to claim 1, further comprising a stem cell bag lifting tool, wherein the stem cell bag lifting tool comprises a lifting rod (4) and a carrier (5) arranged at one end of the lifting rod (4), the sealing port (2) is used for the stem cell bag lifting tool to extend into the liquid nitrogen tank body (1), and the annular boss (23) is provided with a fixing member for preventing the lifting rod (4) from moving.

4. The liquid nitrogen storage tank for stem cell storage according to claim 3, wherein the fixing member comprises a first clamping groove (231) formed in the inner side wall of the annular boss (23), the first clamping groove (231) extends from one end of the annular boss (23) to the other end of the annular boss, the first clamping groove (231) is arranged along the axial direction of the sealing port (2), and the first clamping groove (231) is used for clamping the lifting rod (4).

5. The liquid nitrogen tank for stem cell storage according to claim 4, wherein the lifting rod (4) comprises a first lifting rod section (41), an intermediate connection section (42) and a second lifting rod section (43) which are connected in sequence, and the first lifting rod section (41) and the second lifting rod section (43) are arranged in parallel;

the fixing piece further comprises a second clamping groove (232) formed in the end portion, close to the second sealing opening section (22), of the annular boss (23), the second clamping groove (232) is used for clamping the transition connecting section (42), and the first clamping groove (231) is used for clamping the first lifting rod section (41);

and a third clamping groove (221) is formed in the side wall of the second sealing opening section (22) and/or the side wall of the sealing cover (3), and the third clamping groove (221) is used for clamping the second lifting rod section (43).

6. The liquid nitrogen tank for stem cell storage according to claim 3, wherein a limiting member (44) is disposed at an end of the lifting rod (4) far away from the carrier (5), and the limiting member (44) is used for preventing the lifting rod (4) from sliding down into the liquid nitrogen tank body (1);

one end, close to the annular boss (23), of the sealing cover (3) is provided with an accommodating groove (31) for accommodating the limiting piece (44).

7. The liquid nitrogen tank for stem cell storage according to claim 3, wherein the carrier (5) is a cylindrical container, and the bottom wall and/or the lower part of the side wall of the cylindrical container are/is provided with the opening (51).

8. The liquid nitrogen tank for stem cell storage according to claim 3, wherein a temperature sensor (6) and a liquid level sensor (7) are provided in the liquid nitrogen tank body (1), and the liquid level sensor (7) is not lower than the carrier (5) when the stem cells are in the storage state.

9. The liquid nitrogen tank for stem cell storage according to claim 8, further comprising a display (8) provided on an outer side wall of the liquid nitrogen tank body (1), wherein the display (8) is electrically connected with the temperature sensor (6) and the liquid level sensor (7).

10. The liquid nitrogen tank for stem cell storage according to claim 1, wherein the top of the sealing cover is provided with a hidden handle (9).