CN217657893U

CN217657893U - Freezing tube

Info

Publication number: CN217657893U
Application number: CN202123338176.2U
Authority: CN
Inventors: 李平
Original assignee: Guangzhou Pinzhi Medical Device Co ltd
Current assignee: Guangzhou Pinzhi Medical Device Co ltd
Priority date: 2021-12-27
Filing date: 2021-12-27
Publication date: 2022-10-28
Anticipated expiration: 2031-12-27

Abstract

The utility model discloses a freezing storage tube, which comprises a carrier rod body, an inner sleeve and an outer sleeve, wherein the carrier rod body is hollow, one end of the carrier rod body is of an open structure, the inner cavity of the carrier rod body is provided with a matching part, the bottom end of the matching part is provided with a carrier rod main body, and the carrier rod main body is provided with a carrier piece working area which is used for loading a carrier to be frozen; the inner sleeve is a pipe body structure with an opening at one end, so that the inner sleeve can enter the inner cavity of the rod carrying body through the opening at the open end of the rod carrying body and is in sealing connection with the matching part, and the slide glass working area is sealed in the inner sleeve; the outer sleeve is sleeved outside the inner sleeve and is provided with a through hole communicated with the inner cavity of the outer sleeve. Therefore, liquid nitrogen enters the inner cavity of the outer sleeve from the through hole on the outer sleeve to act on the outer wall of the inner sleeve and freeze the carrier which is sealed in the inner sleeve and is positioned in the slide glass working area, so that a sealed freezing effect is achieved, and the risks of pollution and cross infection caused by biological samples through open storage are avoided.

Description

Freezing tube

Technical Field

The utility model relates to the technical field of medical equipment, in particular to a closed freezing storage tube for low-temperature storage of biological samples such as embryonic stem cells/gametes (ova and sperms)/ovarian tissues and the like.

Background

With the maturation of the vitrification freezing technology, the rapid freezing of embryonic stem cells by the vitrification freezing technology has become an important technology in the reproductive medicine field. The basic principle of the vitrification freezing technology is that firstly, the water in cells is replaced by a high-concentration cryoprotectant solution, and then the cells are rapidly cooled to be converted from a liquid state into an amorphous solid with a shape similar to a glass. When the biological sample is cooled rapidly, water in the cells is replaced, so that the damage of the ice crystal form to the lipid membrane and the cytoskeleton structure of the cells at low temperature can be avoided, the freezing protection effect is achieved, and the viability and the developmental capacity of the frozen and revived biological sample are improved.

Currently, freezing vehicles for storing embryos, gametes and ovarian tissue in the field of assisted reproductive medical devices are mainly freezing rings, freezing straws and freezing carrier rods. However, the three types of freezing storage carriers are all open storage methods, so that the stored biological samples can contact the liquid nitrogen in the liquid nitrogen tank and be polluted by microorganisms or pathogens in the liquid nitrogen, and in addition, a plurality of samples are stored in the same liquid nitrogen tank, so that the risk of cross infection among different biological samples also exists.

SUMMERY OF THE UTILITY MODEL

To prior art not enough, the utility model aims to provide a cryopreserved pipe, it can avoid open storage to arouse the risk of pollution and cross infection.

The purpose of the utility model is realized by adopting the following technical scheme:

the freezing tube comprises:

the carrying rod body is of an open structure at one end, a matching part is arranged in the inner cavity of the carrying rod body, the matching part is provided with a carrying rod main body, and the carrying rod main body is provided with a slide glass working area;

the inner sleeve is of a pipe body structure with an opening at one end, and the inner sleeve can be hermetically connected with the matching part to enable the slide glass working area to be sealed in the inner sleeve.

Further, the inner sleeve is provided with a sleeve portion, the inner wall of the sleeve portion is provided with an inner conical surface, the outer wall of the matching portion is provided with an outer conical surface, and the sleeve portion and the matching portion can form interference fit through matching between the inner conical surface and the outer conical surface.

Furthermore, a thread is arranged on the side wall of the open end inner cavity of the carrying rod body along the circumferential direction of the carrying rod body, and a lug capable of being matched with the thread is arranged on the outer wall of the sleeve part along the circumferential direction of the sleeve part.

Furthermore, the utility model discloses still including the outer tube, the outer tube pass through buckle structure with the sleeve part joint will interior sleeve pipe seals in the outer tube, still be provided with on the outer tube with the communicating through-hole of self inner chamber.

Furthermore, the buckle structure comprises a plurality of protrusions arranged on the outer wall of the sleeve portion and a plurality of clamping grooves circumferentially arranged on the side wall of the inner cavity of the outer sleeve, and the protrusions are arranged along the circumferential direction of the sleeve portion and are matched with the clamping grooves one by one.

Furthermore, a guide groove for guiding the protrusion to enter the clamping groove is further formed in the side wall of the inner cavity of the outer sleeve.

Furthermore, a slot is formed in the matching portion and used for installing an identification key, and the identification key is used for marking the direction of the slide glass working area.

Further, the bottom of the inner chamber of outer tube is provided with the installation department, the installation department is used for installing the balancing weight.

Furthermore, a limiting part is arranged on the side wall of the inner cavity of the outer sleeve, and the limiting part is used for limiting the degree of freedom of the balancing weight in the length direction of the outer sleeve.

Furthermore, a plurality of reinforcing ribs are arranged at the bottom of the outer wall of the outer sleeve, and each reinforcing rib is arranged along the circumferential direction of the outer sleeve.

Compared with the prior art, the beneficial effects of the utility model reside in that:

the utility model discloses a cryopreserving pipe is when using, the carrier loading that will treat the refrigeration is at slide glass workspace, with interior sleeve pipe through the opening of self in the open one end of carrying the body of rod get into the inner chamber of carrying the body of rod and carry the cooperation portion sealing connection of the body of rod and make slide glass workspace seal at interior sleeve intraductal, then through freezing fast in the liquid nitrogen, the liquid nitrogen acts on interior sheathed tube outer wall and the freezing carrier that seals at interior sleeve pipe just is located slide glass workspace, thereby reach closed freezing effect, and then avoid biological sample to arouse through open storage and pollute and cross infection's risk.

Drawings

FIG. 1 is a cross-sectional view of the cryopreservation tube of the present invention;

FIG. 2 is a schematic view of the three-dimensional structure of the cryopreservation tube of the present invention;

FIG. 3 is a schematic view of the exploded structure of the cryopreservation tube of the present invention;

fig. 4 is a schematic structural view of a rod carrying body according to an embodiment of the present invention;

fig. 5 is a schematic structural view of an inner sleeve according to an embodiment of the present invention;

fig. 6 is a schematic structural diagram of an outer sleeve according to an embodiment of the present invention.

In the figure: 10. a rod carrying body; 101. a fitting portion; 102. a carrier bar body; 1021. a slide work area; 103. a thread; 104. a slot; 105. an operation section; 20. an inner sleeve; 201. a sleeve portion; 2011. a lug; 2012. a protrusion; 202. a body portion; 30. an outer sleeve; 301. a through hole; 302. a card slot; 303. a guide groove; 304. an installation part; 305. salient points; 306. reinforcing ribs; 40. a balancing weight; 50. the key is identified.

Detailed Description

In the following, the present invention is described with priority in conjunction with the accompanying drawings and the detailed description, and it should be noted that, in the premise of no conflict, the embodiments or technical features described below may be arbitrarily combined to form a new embodiment.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "horizontal", "vertical", "top", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

In the description of the present invention, it should be noted that unless explicitly stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to specific situations.

The implementation mode is as follows:

referring to fig. 1-6, the present invention shows a cryopreservation tube, which includes a rod-carrying body 10, an outer cannula 30 and an inner cannula 20, wherein the rod-carrying body 10 is of an open structure at one end, a matching portion 101 is provided in an inner cavity of the rod-carrying body 10, a rod-carrying main body 102 is provided at a bottom end of the matching portion 101, a slide work area 1021 is provided on the rod-carrying main body 102, and the slide work area 1021 is used for loading biological samples such as embryonic stem cells/gametes (eggs, sperms)/ovarian tissues and the like) to be frozen; the inner sleeve 20 is a tube structure with an opening at one end, and the inner sleeve can be hermetically connected with the matching part to seal the slide work area 1021 in the inner sleeve 20; the outer sleeve 30 is sleeved outside the inner sleeve 20 and is provided with a through hole 301 communicated with the inner cavity of the outer sleeve. It can be understood, the utility model discloses a cryopreserved pipe is when using, will treat that frozen carrier loads at slide glass workspace 1021, with interior sleeve pipe 20 through the opening of self in slide glass body 10 open one end get into slide glass body 10 the inner chamber with slide glass body 10 the cooperation portion 101 sealing connection and make slide glass workspace 1021 seal including sleeve pipe 20 in, again with outer tube 30 cover locate interior sleeve pipe 20 outside on, at last will the utility model discloses a cryopreserved pipe is arranged in the liquid nitrogen and is frozen fast, the inner chamber that the through-hole 301 on outer tube 30 followed the outer tube 30 gets into outer tube 30 acts on interior sleeve pipe 20's outer wall and freezes the carrier that seals including in sleeve pipe 20 and be located slide glass workspace 1021 to reach closed freezing effect, and then avoid biological sample to arouse pollution and cross infection's problem through open storage.

In this embodiment, the inner bushing 20 includes a hollow tube 202 and a sleeve 201, the tube 202 is connected to the sleeve 201, and an inner cavity of the tube 202 is communicated with an inner cavity of the sleeve 201, that is, the tube 202 is hollow and has an open end, the open end of the tube 202 is connected to the sleeve 201, and an end of the tube 202 facing away from the sleeve 201 is closed. The sleeve portion 201 is configured to be sleeved on the matching portion 101 of the inner cavity of the rod carrying body 10, and is in taper connection with the outer wall of the matching portion 101, so that an interference fit connection is formed between the inner wall of the sleeve portion 201 and the outer wall of the matching portion 101, and the inner sleeve 20 is in sealed connection with the matching portion 101. Therefore, the inner wall of the sleeve portion 201 is provided with an inner conical surface, the outer wall of the matching portion 101 is provided with an outer conical surface, and the sleeve portion 201 and the matching portion 101 are matched through the inner conical surface and the outer conical surface to realize the sealing connection effect of the inner sleeve 20 and the matching portion 101.

It should be noted that, compared with the sealing structure implemented by thermal effect or adhesion or ultrasonic welding, the sealing structure implemented by the inner sleeve 20 and the matching portion 101 is safer, and does not generate heat energy or ultrasonic waves to cause potential damage to the embryo loaded in the slide work area 1021.

In this embodiment, the matching portion 101 is further provided with a slot 104, the slot 104 is used for installing the identification key 50, and the identification key 50 is used for marking the direction of the slide work area 1021; specifically, the slot 104 is opened from the bottom end of the mating portion 101 to the top of the inner cavity of the slide bar body 10, and it should be noted that the slot 104 is not disposed on the outer wall of the mating portion 101, so as to avoid affecting the sealing connection between the mating portion 101 and the sleeve portion 201. It will also be appreciated that the identification key 50 facilitates a worker to quickly identify the orientation of the slide work area 1021 to facilitate placement of a carrier to be frozen in the slide work area 1021.

In addition, a thread 103 is arranged on the side wall of the inner cavity of the carrier rod body 10 along the circumferential direction of the carrier rod body, a lug 2011 which can be matched with the thread 103 is arranged on the outer wall of the sleeve portion 201 along the circumferential direction of the sleeve portion, and the inner sleeve 20 is connected with the inner cavity of the carrier rod body 10 through the matching of the thread 103 and the lug 2011; the fitting part 101 can be driven to move along the axial direction of the inner sleeve 20 by rotating the carrying rod body 10, and the interference fit between the fitting part 101 and the inner sleeve 20 can be realized; in addition, by providing the number of turns of the thread 103, an interference fit between the mating portion 101 and the inner sleeve 20 is ensured after the lug 2011 is rotationally moved into position relative to the thread.

In this embodiment, the outer sleeve 30 is engaged with the sleeve portion 201 by a snap structure to enclose the inner sleeve 20 in the outer sleeve 30; the snap structure includes a plurality of protrusions 2012 arranged on the outer wall of the sleeve portion 201 and a plurality of slots 302 circumferentially arranged on the inner cavity side wall of the outer sleeve 30, each protrusion 2012 is arranged along the circumference of the sleeve portion 201, and the slots 302 are matched with the protrusions 2012 one by one.

It can be understood that the connection between the outer sleeve 30 and the carrier rod body 10 is realized by the engagement of the protrusions 2012 on the sleeve portion 201 and the locking grooves 302 on the outer sleeve 30. Specifically, three protrusions 2012 are arranged on the outer wall of the sleeve portion 201, each protrusion 2012 is uniformly arranged along the circumferential direction of the sleeve portion 201 at intervals, three slots 302 are also arranged on the side wall of the inner cavity of the outer sleeve 30, and each slot 302 corresponds to each protrusion 2012 one by one, so that the connection stress between the outer sleeve 30 and the inner sleeve 20 is uniform, and the outer sleeve 30 does not shake. Of course, in other embodiments, the inventor may reasonably change the number of the protrusions 2012 and the slots 302 according to the actual situation of the cryopreservation tube of the present invention, which is not limited herein. In addition, each of the locking slots 302 is disposed to penetrate the inner and outer wall structures of the outer sleeve 30, so that the protrusions 2012 are locked in the locking slots 302 more stably.

In this embodiment, in order to facilitate the engagement between the slot 302 and the protrusion 2012, a guide groove 303 is further disposed on a side wall of the inner cavity of the outer sleeve 30 for guiding the protrusion 2012 to enter the slot 302, and the protrusion 2012 is guided by the guide groove 303 to enter the slot 302, so as to achieve a quick connection effect between the outer sleeve 30 and the inner sleeve 20. Specifically, the top end of each clamping groove 302 is provided with a guide groove 303, and when the outer sleeve 30 is sleeved outside the inner sleeve 20, the protrusion 2012 can quickly and accurately enter the clamping groove 302 through the guide grooves 303 to be clamped with the clamping groove 302, so that the quick connection effect of the outer sleeve 30 and the inner sleeve 20 is realized.

It is worth explaining that, when outer tube 30 and interior sleeve pipe 20 were connected, outer tube 30 still with carry the open one end butt of the body of rod 10, including interior sleeve pipe 20 seals completely with the inner chamber of carrying the body of rod 10 like this, as required, the inventor can also set up the sealing washer in outer tube 30 and the butt department of carrying the body of rod 10, in order to further promote the utility model discloses freeze the sealed effect of depositing the pipe.

In this embodiment, the inner chamber of outer tube 30 is provided with balancing weight 40, increases through this configuration piece the utility model discloses freeze the weight of depositing the pipe, in order to prevent the utility model discloses a freeze and deposit the pipe and float in the liquid nitrogen. Wherein, the bottom of outer tube 30 is provided with installation portion 304, and balancing weight 40 is installed in installation portion 304. Because the cryogenic vial is at a lower temperature in the liquid nitrogen, a certain gap is left between the counterweight 40 and the mounting portion 304, preventing the outer sleeve 30 from being cracked by the configuration block in the freezing process.

Of course, in order to prevent the weight 40 from falling out of the mounting portion 304, a limiting portion is disposed on a side wall of the inner cavity of the outer sleeve 30, and the limiting portion limits the degree of freedom of the weight 40 in the length direction of the outer sleeve 30, so as to prevent the weight 40 from falling out of the mounting portion 304. Specifically, the limiting part comprises three salient points 305, each salient point 305 is arranged on the side wall of the inner cavity of the outer sleeve 30 and is uniformly arranged along the circumferential direction at intervals, and the contact surface of each salient point 305 and the outer wall of the balancing weight 40 is a spherical surface, so that when the balancing weight 40 is installed, the balancing weight 40 can be conveniently installed in the installation part 304, and the balancing weight 40 cannot be interfered to influence the installation of the balancing weight 40; after the weight 40 is installed in the installation portion 304, the weight 40 can be prevented from falling out of the installation portion 304 by the three bumps 305.

In this embodiment, the one end of carrying the body of rod 10 dorsad cooperation portion 101 is provided with operating portion 105, and operating portion 105 is used for connecting operating handle, and this operating portion 105 is eight star shape recesses in, when needs take out the carrier on the slide glass workspace 1021 and unfreeze, can break away from interior sleeve pipe 20 and the cooperation portion 101 of carrying the body of rod 10 through operating handle in eight star shape recess internal rotations to it unfreezes to take out the carrier on the slide glass workspace 1021. In addition, the outer wall of the middle and rear part of the carrying rod body 10 can be used for sticking information data of patients so as to be convenient for distinguishing.

In this embodiment, the bottom of the outer wall of the outer sleeve 30 is provided with a plurality of reinforcing ribs 306, each reinforcing rib 306 is arranged along the circumferential direction of the outer sleeve 30, and the reinforcing ribs 306 can enhance the structural strength of the outer sleeve 30; furthermore, because the utility model discloses a when freezing in the liquid nitrogen and depositing the pipe, the bottom of outer tube 30 is connected with the cooperation of storage box, is also that this strengthening rib 306 is connected with the spacing groove cooperation in the storage box, so when taking out the carrier on slide glass workspace 1021 and unfreezing and need rotate the carrier body of rod 10 and interior sleeve pipe 20 break away from, this strengthening rib 306 can also play spacing effect, prevents that outer tube 30 from rotating and is convenient for break away from interior sleeve pipe 20 and carrier body of rod 10.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention cannot be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are all within the protection scope of the present invention.

Claims

1. The cryopreserved pipe, its characterized in that, including:

the slide bar comprises a bar carrying body (10), wherein one end of the bar carrying body (10) is of an open structure, a matching part (101) is arranged in an inner cavity of the bar carrying body (10), a bar carrying body (102) is arranged on the matching part (101), and a slide glass working area (1021) is arranged on the bar carrying body (102);

the inner sleeve (20), inner sleeve (20) is equipped with the open-ended body structure for one end, inner sleeve (20) can with cooperation portion (101) sealing connection and make slide glass workspace (1021) seal in inner sleeve (20).

2. The cryopreservation tube of claim 1, wherein: the inner sleeve (20) is provided with a sleeve part (201), the inner wall of the sleeve part (201) is provided with an inner conical surface, the outer wall of the matching part (101) is provided with an outer conical surface, and the sleeve part (201) and the matching part (101) can form interference fit through the matching between the inner conical surface and the outer conical surface.

3. The cryopreservation tube of claim 2, wherein: the side wall of the open end inner cavity of the carrying rod body (10) is provided with a thread (103) along the circumferential direction of the carrying rod body, and the outer wall of the sleeve portion (201) is provided with a lug (2011) which can be matched with the thread (103) along the circumferential direction of the sleeve portion.

4. The cryopreservation tube of claim 3 wherein: the outer sleeve (30) is clamped with the sleeve part (201) through a clamping structure, the inner sleeve (20) is sealed in the outer sleeve (30), and a through hole (301) communicated with the inner cavity of the outer sleeve (30) is further formed in the outer sleeve (30).

5. The cryopreservation tube of claim 4, wherein: the buckling structure comprises a plurality of protrusions (2012) arranged on the outer wall of the sleeve portion (201) and a plurality of clamping grooves (302) circumferentially arranged on the side wall of the inner cavity of the outer sleeve (30), and the protrusions (2012) are circumferentially arranged along the sleeve portion (201) and are matched with the clamping grooves (302) one by one.

6. The cryopreservation tube of claim 5 wherein: the side wall of the inner cavity of the outer sleeve (30) is also provided with a guide groove (303) for guiding the bulge (2012) to enter the clamping groove (302).

7. The cryopreservation tube of claim 1, wherein: a slot (104) is formed in the matching portion (101), the slot (104) is used for installing an identification key (50), and the identification key (50) is used for marking the direction of the slide work area (1021).

8. The cryopreservation tube of claim 4, wherein: the bottom of the inner cavity of the outer sleeve (30) is provided with an installation part (304) for installing a balancing weight (40).

9. The cryopreservation tube of claim 8, wherein: the lateral wall of the inner cavity of the outer sleeve (30) is provided with a limiting part, and the limiting part is used for limiting the degree of freedom of the balancing weight (40) in the length direction of the outer sleeve (30).

10. The cryopreservation tube of claim 4, wherein: the bottom of the outer wall of the outer sleeve (30) is provided with a plurality of reinforcing ribs (306), and each reinforcing rib (306) is arranged along the circumferential direction of the outer sleeve (30).