CN215775142U - Safe and be convenient for a large amount of program cooling box of cryopreserving living cells - Google Patents
Safe and be convenient for a large amount of program cooling box of cryopreserving living cells Download PDFInfo
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- CN215775142U CN215775142U CN202122284527.XU CN202122284527U CN215775142U CN 215775142 U CN215775142 U CN 215775142U CN 202122284527 U CN202122284527 U CN 202122284527U CN 215775142 U CN215775142 U CN 215775142U
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Abstract
The utility model discloses a safe program cooling box convenient for freezing and storing a large number of living cells, which comprises a cover body and a box body; the method is characterized in that: a support plate is arranged at the bottom in the box body, and a plurality of through holes are formed in the support plate; the lower end of the carrier plate is connected with a bracket corresponding to the through hole; a pipe placing layer is arranged on the bracket; a program cooling and freezing device is arranged between the bracket and the bottom of the box body; the box body is made of transparent materials; the front end of the box body is provided with a groove, and a label is inserted in the groove; the periphery of the box body is provided with convex edges, and the bottom of the box body is provided with concave edges. The utility model saves the working time of manual gradient cooling for freezing and storing living cells, reduces errors, saves time and labor, facilitates the operation of experimenters, and can prolong the service time of organic solvents and reduce the exposure risk of the experimenters by preventing liquid leakage.
Description
Technical Field
The utility model relates to the field of sample preservation facilities, in particular to a program cooling box which is safe and convenient for freezing and preserving a large number of living cells.
Background
In life science and medical experiments, artificial gradient cooling in the traditional method is to place living cells containing frozen stock solution at 4 ℃, 20 ℃ and 80 ℃ below zero for 2 hours respectively, and finally place the cells under the condition of liquid nitrogen for long-term storage or short-term storage at 80 ℃ below zero, so that the whole operation time is long, the process is complex, and errors and frostbite of medical personnel are easily caused.
The conventional common product is a cell program cooling cryopreservation box containing isopropanol, which can realize that living cells are gradually cooled at the speed of 1 ℃ per minute, so that digested living cells are uniformly mixed by using a cryopreservation solution and are subpackaged into cryopreservation tubes, the cryopreservation tubes can be directly placed in an ultra-low temperature refrigerator at-80 ℃ after being placed in the program cooling cryopreservation box, programmed cooling and preservation of the living cells are realized in one step, and the following defects are found in the using process: (1) the isopropanol between the material placing layer and the box body is easy to leak and volatilize after being transported for a long distance, and the leakage of the organic solvent can dissolve the marks made by the marker pens on the tube, thereby influencing the information recording; the freezing storage box is not ideal for long-term use due to leakage and volatilization, and organic solvents need to be purchased and supplemented frequently, so that reagents and workload are wasted; (2) because the organic solvent and the plastic freezing storage box expand with heat and contract with cold under the condition of low temperature, the phenomenon that the cover cannot be twisted during taking is often caused; (3) the existing cylindrical box body has fewer holes, and wastes space when a large number of square sample boxes are stored simultaneously. (4) Live cell cryopreservation cartridges are not conveniently labeled and often lack differentiation.
In order to make up for the defects, the tube placing layer is provided with the injection hole for supplementing isopropanol, and the connected piston is designed to be selectively opened, so that the volatilization of the organic solvent in the room temperature placing of the program cooling box, namely when the program cooling box is not used, the leakage in manual or motor vehicle transportation is prevented, and the waste of the organic solvent is avoided; the program cooling box for freezing and storing living cells is designed to be square, the gland is designed, the opening is convenient even if the box expands with heat and contracts with cold, and the square program cooling box similar to a common sample box can be designed with more pipe placing holes; the top and the bottom edge are respectively provided with the convex edge and the concave edge, which is beneficial to stacking the program cooling boxes made of low-temperature plastic materials, is not easy to slide off, and can be stacked with common sample storage boxes or placed in a metal storage rack used in a refrigerator. A clamping groove for placing a label is arranged at the front end of the box body, and sample information can be marked and replaced.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects of the prior art, the utility model provides the program cooling box which is safe and convenient for freezing and storing a large number of living cells, and solves the defects of the prior art.
In order to achieve the purpose, the utility model adopts the following technical scheme:
a safe and convenient procedure cooling box for freezing and storing a large amount of living cells comprises a cover body and a box body; the method is characterized in that: a support plate is arranged at the bottom in the box body, and a plurality of through holes are formed in the support plate; the lower end of the carrier plate is connected with a bracket corresponding to the through hole; a pipe placing layer is arranged on the bracket; a program cooling and freezing device is arranged between the bracket and the bottom of the box body; the box body is made of transparent materials; the front end of the box body is provided with a groove, and a label is inserted in the groove; the periphery of the box body is provided with convex edges, and the bottom of the box body is provided with concave edges.
According to the optimized scheme, the program cooling and freezing device comprises a freezing seat arranged at the bottom in the box body, and a freezing interlayer is arranged in the freezing seat; a vertical channel with the freezing interlayer is arranged on the freezing seat; a connecting pipe is arranged between the support plate and the vertical channel, and a plug is arranged on the connecting pipe.
According to the optimized scheme, a sponge lining is arranged between the freezing seat and the interior of the box body.
According to the optimized scheme, scale marks are symmetrically arranged on the side face of the box body.
According to the optimized scheme, a positioning and rotating device is arranged between the box body and the cover body; the positioning and rotating device comprises a connecting shaft arranged at the upper part of the side surface of the box body, the connecting shaft penetrates through one side of the cover body, and the cover body is movably connected with the connecting shaft; a circular baffle is arranged at the outer side end of the connecting shaft, and a plurality of through holes are formed in the circular baffle; the cover body is provided with a groove corresponding to the through hole; the through hole is fixedly connected with the groove through a bolt.
Due to the adoption of the technology, compared with the prior art, the utility model has the beneficial effects that:
the utility model is designed to be safer in structure, the organic solvent is not easy to leak and volatilize, the utility model is convenient for long-distance transportation, the gradient cooling function is realized by pouring the isopropanol into the freezing interlayer, the temperature can be gradually reduced at the speed of 1 ℃ per minute by placing the refrigerator at the temperature of-80 ℃ ultra-low temperature, the utility model meets the requirements of freezing and storing the living cells, saves the working time of artificial gradient cooling for freezing the living cells, reduces the errors, saves time and labor, is convenient for the operation of experimenters, and simultaneously, the liquid leakage is not easy to occur, and can prolong the use time of the organic solvent and reduce the exposure risk of the experimenters.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a carrier plate, a carrier and a tube accommodating layer according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a carrier plate, a freezing interlayer and a freezing seat according to an embodiment of the present invention;
FIG. 4 is a schematic side view of the structure of FIG. 1;
fig. 5 is a schematic view of the inner side structure of the cover.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Examples
As shown in fig. 1-4, a programmed cooling box for safely and conveniently freezing and storing a large number of living cells comprises a cover body 5 and a box body 1. A support plate 6 is arranged at the bottom in the box body 1, and a plurality of through holes are formed in the support plate 6. The lower end of the carrier plate 6 is connected with a bracket 11 corresponding to the through hole; the bracket 11 is provided with a tube placing layer 10. A program cooling and freezing device is arranged between the bracket 11 and the bottom of the box body 1. The program cooling and freezing device comprises a freezing seat 8 arranged at the bottom in the box body, and a freezing interlayer 9 is arranged in the freezing seat 8. And a vertical channel between the freezing seat and the freezing interlayer is arranged on the freezing seat. A connecting pipe 7 is arranged between the support plate and the vertical channel, and a plug 4 is arranged on the connecting pipe. A sponge lining is arranged between the freezing seat and the box body. The plug 4 is a rubber plug. When the user injects isopropanol by a needle tube, the rubber plug is opened during the procedure cooling, so that the air is conveniently exchanged by expanding with heat and contracting with cold, and the rubber plug is sealed after the use, so that the leakage is not easy to occur.
The box body 1 is made of transparent materials; the front end of the box body 1 is provided with a groove, and a label is inserted in the groove. The import of recess can set up the opening that can stretch out and draw back, avoids dropping of label, and the opening has flexible performance simultaneously, can play the condensation of avoiding cold air to get into and causing, effectively protects the label. Protruding edges are arranged on the periphery of the box body 1, and recessed edges are arranged at the bottom of the box body. This kind of peripheral protruding, the design of bottom indent, it is more firm when the stromatolite is placed, takes safety simultaneously, this is because wear gloves and take the box of taking ice and drop easily, causes the damage of box body. In addition, as shown in fig. 5, the inner side surface of the side edge of the cover body is provided with a clamping groove, so that the box body and the cover body are better fixed through the clamping groove.
The side of the box body is symmetrically provided with scale marks 3. The function of the graduation mark is to aim at the inner freezing interlayer, so that the volume of the poured isopropanol is convenient to observe.
A positioning and rotating device is arranged between the box body and the cover body, and the positioning and rotating device is an additional structure. When some box bodies and cover bodies are heavier, a mechanism can be designed. If the box body and the cover body are made of light materials, the design is not needed. The positioning and rotating device comprises a connecting shaft 23 arranged on the upper part of the side surface of the box body, and the connecting shaft 23 penetrates through one side of the cover body and is movably connected with the cover body. The outer side end of the connecting shaft 23 is provided with a circular baffle 21, and the circular baffle is provided with a plurality of through holes. And the cover body is provided with a groove corresponding to the through hole. The through hole is fixedly connected with the groove through a bolt 22.
The utility model is designed to be safer in structure, the organic solvent is not easy to leak and volatilize, the utility model is convenient for long-distance transportation, the gradient cooling function is realized by pouring the isopropanol into the freezing interlayer, the temperature can be gradually reduced at the speed of 1 ℃ per minute by placing the refrigerator at the temperature of-80 ℃ ultra-low temperature, the utility model meets the requirements of freezing and storing the living cells, saves the working time of artificial gradient cooling for freezing the living cells, reduces the errors, saves time and labor, is convenient for the operation of experimenters, and simultaneously, the liquid leakage is not easy to occur, and can prolong the use time of the organic solvent and reduce the exposure risk of the experimenters.
The label is marked, and the transparent box body is matched for observation and identification, so that the isopropanol is convenient and reliable to fill, and meanwhile, the sponge lining is beneficial to gas exchange of expansion with heat and contraction with cold of the organic solvent, and an operator can be ensured to visually and clearly acquire the related information of the biological sample in the cryopreservation box.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution of the present invention and the inventive concept thereof should be covered by the scope of the present invention.
Claims (5)
1. A safe and convenient procedure cooling box for freezing and storing a large amount of living cells comprises a cover body and a box body;
the method is characterized in that:
a support plate is arranged at the bottom in the box body, and a plurality of through holes are formed in the support plate; the lower end of the carrier plate is connected with a bracket corresponding to the through hole; a pipe placing layer is arranged on the bracket;
a program cooling and freezing device is arranged between the bracket and the bottom of the box body;
the box body is made of transparent materials; the front end of the box body is provided with a groove, and a label is inserted in the groove; the periphery of the box body is provided with convex edges, and the bottom of the box body is provided with concave edges.
2. The programmed cooling cartridge of claim 1, wherein said cartridge further comprises: the program cooling and freezing device comprises a freezing seat arranged at the bottom in the box body, and a freezing interlayer is arranged in the freezing seat; a vertical channel with the freezing interlayer is arranged on the freezing seat; a connecting pipe is arranged between the support plate and the vertical channel, and a plug is arranged on the connecting pipe.
3. The programmed cooling cartridge of claim 2, wherein said cartridge further comprises: a sponge lining is arranged between the freezing seat and the box body.
4. The programmed cooling cartridge of claim 3, wherein said cartridge further comprises: the side of the box body is symmetrically provided with scale marks.
5. The programmed cooling cartridge of claim 4, wherein said cartridge further comprises: a positioning rotating device is arranged between the box body and the cover body; the positioning and rotating device comprises a connecting shaft arranged at the upper part of the side surface of the box body, the connecting shaft penetrates through one side of the cover body, and the cover body is movably connected with the connecting shaft; a circular baffle is arranged at the outer side end of the connecting shaft, and a plurality of through holes are formed in the circular baffle; the cover body is provided with a groove corresponding to the through hole; the through hole is fixedly connected with the groove through a bolt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122284527.XU CN215775142U (en) | 2021-09-18 | 2021-09-18 | Safe and be convenient for a large amount of program cooling box of cryopreserving living cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122284527.XU CN215775142U (en) | 2021-09-18 | 2021-09-18 | Safe and be convenient for a large amount of program cooling box of cryopreserving living cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215775142U true CN215775142U (en) | 2022-02-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122284527.XU Active CN215775142U (en) | 2021-09-18 | 2021-09-18 | Safe and be convenient for a large amount of program cooling box of cryopreserving living cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215775142U (en) |
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2021
- 2021-09-18 CN CN202122284527.XU patent/CN215775142U/en active Active
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