CN220640884U

CN220640884U - Biological sample liquid nitrogen storage tank

Info

Publication number: CN220640884U
Application number: CN202322243370.5U
Authority: CN
Inventors: 赵竹然; 石程; 金恩忠; 徐帅; 王一夫; 张世敏; 李铭辉
Original assignee: Peking University Peoples Hospital
Current assignee: Peking University Peoples Hospital
Priority date: 2023-08-21
Filing date: 2023-08-21
Publication date: 2024-03-22
Anticipated expiration: 2033-08-21

Abstract

The utility model discloses a biological sample liquid nitrogen storage tank which comprises a tank body, a sealing cover, a storage tray and a storage tray rotating mechanism. A liquid nitrogen storage bin is arranged in the tank body; the sealing cover is arranged at the upper part of the tank body; the storage disc rotating mechanism comprises a central rotating shaft penetrating through the tank body in the horizontal direction and a storage disc bracket; the storage tray is arranged on the storage tray bracket; the central rotating shaft is fixedly connected with the object placing disc bracket; the object placing disc rotating mechanism is used for adjusting the position of the object placing disc. The utility model improves the problem that the existing liquid nitrogen storage tank is inconvenient for accessing the biological sample, reduces the exposure time of the biological sample outside the liquid nitrogen in the access process, is beneficial to maintaining the activity of the biological sample, reduces the evaporation rate of the liquid nitrogen and improves the working efficiency.

Description

Biological sample liquid nitrogen storage tank

Technical Field

The utility model belongs to the technical field of liquid nitrogen storage, and particularly relates to a biological sample liquid nitrogen storage tank.

Background

Liquid nitrogen refers to liquid nitrogen, which is a very low temperature liquid. At normal temperature, the boiling point of liquid nitrogen is-196.56 ℃. Liquid nitrogen does not react with most metals nor oxygen, and is often used to store biological samples such as cells, tissues, bacteria, and the like. In some basic experiments and clinic, embryo vitrification freezing technology is due to the ultralow temperature characteristic of liquid nitrogen, and can effectively prevent the sample from deteriorating and the microorganism from growing. Therefore, a storage tank filled with liquid nitrogen is generally used to store the biological sample.

However, a plurality of different samples are usually placed in the liquid nitrogen storage tank at the same time, the placed biological samples are usually placed in layers, and when a worker places or takes out the samples placed on the lower layer, the worker needs to take out the samples on the upper layer first, and then put back the samples on the upper layer after taking out the samples on the lower layer. Therefore, the traditional layered biological sample liquid nitrogen storage tank is complex in operation, and the activity of the upper biological sample can be damaged due to the fact that the upper biological sample is exposed to the air during repeated taking and placing. In addition, in the process of taking and placing samples, workers need to stretch hands into the storage tank, so that the hands are easily frostbitten, and certain potential safety hazards exist.

Therefore, a biological sample liquid nitrogen storage tank capable of conveniently taking and placing samples and reducing potential frostbite hazards is needed.

Disclosure of Invention

In order to solve the technical problems, the utility model solves the technical problems as follows:

the liquid nitrogen storage tank for biological samples is characterized by comprising a tank body, a sealing cover, a storage tray and a storage tray rotating mechanism.

A liquid nitrogen storage bin is arranged in the tank body; the sealing cover is arranged at the upper part of the tank body; the object placing disc rotating mechanism is used for adjusting the position of the object placing disc; the storage disc rotating mechanism comprises a central rotating shaft penetrating through the tank body in the horizontal direction and a storage disc support, and the central rotating shaft is fixedly connected with the storage disc support; the storage tray is arranged on the storage tray bracket.

Further, the storage tray comprises a storage tray body and a storage tray cover, and the storage tray body is detachably and hermetically connected with the storage tray cover. The tray cover of the storage tray can prevent cell pollution and maintain good cell storage environment.

Further, the number of the storage trays is more than two, and preferably, 2, 3, 4, 5 or 6 storage trays are adopted.

Further, the center of the object placing tray bracket is provided with a shaft hole, and the center rotating shaft penetrates through the shaft hole.

Further, there is no gap between the shaft hole and the central rotating shaft.

Further, the design of shaft hole can improve mechanical structure's intensity, precision and stability, and convenient equipment saves space simultaneously.

Further, the center rotating shaft is fixed with the object placing disc support through fixed connection, so that the object placing disc support has high fixing force and stability, and stability and accuracy of the rotating shaft in the operation process can be ensured.

Further, the center rotating shaft penetrates through the tank body in the horizontal direction, and the center rotating shaft can drive the object placing disc support to rotate by 360 degrees when rotating, so that the position adjustment operation of the object placing disc is more convenient, and the overall stability of the device is improved.

Further, the object placing tray support is provided with a plurality of object placing tray support arms by taking the shaft hole as a starting point; the object placing tray support arms are radially distributed around the shaft hole as the center.

Further, the article-placing tray support takes the shaft hole as a supporting point, a plurality of article-placing tray support arms are radially distributed outwards from the shaft hole, and every two article-placing tray support arms radially expand outwards at a certain angle from the shaft hole. The design can enable the object placing tray support to provide support at a plurality of angles, so that stress is better dispersed, and support and stability are improved.

Further, the object placing tray support arm is provided with an object placing tray support hole.

Further, a circular boss is arranged on the side face of the storage disc, and the boss is in clearance fit with the storage disc support hole.

Further, the tray cover of the storage tray is a transparent cover, so that the position of a required biological sample can be conveniently and rapidly found.

Further, the rocker is positioned outside the tank body; the rocker is connected with one end of a central rotating shaft penetrating through the tank body.

Further, the rocker comprises a connecting rod, a fixing device and a connecting piece, and the rocker is simple in structure operation and good in operability.

Further, a lighting device is arranged in the liquid nitrogen storage bin; in a liquid nitrogen environment, the common hot light source illumination may interfere with the biological sample, so that the cold light source illumination may be selected.

Further, the lighting device may employ LED lamps for illumination or fluorescent lamps. Can provide durable illumination and avoid the influence of excessive temperature on the liquid nitrogen environment.

Further, a tool placement box for storing and clamping tools is arranged on the tank body, and the tool placement box is of an open structure and can be used for storing special tools such as forceps, cell clamps or liquid nitrogen special pipettors.

Furthermore, the special clamping tool is made of a cold insulation material, so that the hand can be prevented from being frostbitten when liquid nitrogen is operated.

Further, the biological sample liquid nitrogen reservoir can be designed in different shapes depending on the application, including but not limited to cubes, rectangular solids, cylindrical and spherical structures.

Further, a liquid nitrogen storage bin is arranged inside the tank body.

Further, the inner wall of the tank body is provided with a heat insulation layer, and the heat insulation layer in the tank body can be made of foamed polystyrene, glass wool and aluminum silicate wool, so that the materials can effectively keep the temperature of liquid nitrogen, can resist the influence of external heat on the liquid nitrogen, and reduce the heat loss of the liquid nitrogen.

Further, the upper part of the tank body is provided with a sealing cover, the sealing cover is made of transparent material, glass manufactured by borosilicate can be adopted, the glass material has high transparency, and a sealing ring is arranged around the sealing cover.

Further, the tank body is formed by processing high-quality stainless steel. The stainless steel material with high quality is used, has extremely strong corrosion resistance and durability, can withstand high pressure and long-term storage, and is safe and reliable.

Further, the tank body and the sealing cover are welded. The whole structure is more stable, and a more suitable environment is provided for storing biological samples.

The basic use process of biological sample liquid nitrogen storage tank is, biological sample liquid nitrogen storage tank is inside to be full of liquid nitrogen, sees through sealed lid and puts the interior sample cell name of thing dish, rotates the rocker to center pivot drive puts thing dish support motion, and then changes and put thing dish position, will be required put thing dish and rotate to the position that is close to the sealed lid opening of liquid nitrogen tank. The sealing cover is opened, and the tool is used for taking out the needed biological sample.

Compared with the prior art, the utility model has the beneficial effects that:

1. the biological sample liquid nitrogen storage tank has a simple structure, can conveniently store and take biological samples at different positions in the tank, improves the working efficiency, and reduces the risk of frostbite of staff in long-time operation.

2. The biological sample liquid nitrogen storage tank provided by the utility model has the advantages that the position movement of the storage tray in the tank is conveniently realized, an operator can conveniently adjust the position of biological sample cells in the liquid nitrogen tank according to the needs, further the rapid taking and placing are realized, the exposure time of a sample outside the liquid nitrogen tank caused by the position adjustment is reduced, the inactivation of the sample is avoided, and the evaporation of liquid nitrogen is reduced.

3. The biological sample liquid nitrogen storage tank sealing cover is made of transparent materials and is provided with the lighting device, so that a worker can clearly see the name of a sample during operation, and the process of taking and placing the target biological sample can be completed more quickly and accurately.

Drawings

The utility model will be further described with reference to the accompanying drawings and examples, in which:

FIG. 1 is a schematic diagram of the overall structure of a biological sample liquid nitrogen storage tank according to the present utility model;

FIG. 2 is a front view of a biological sample liquid nitrogen reservoir of the present utility model;

FIG. 3 is a left side view of a biological sample liquid nitrogen reservoir of the present utility model;

FIG. 4 is a top view of a biological sample liquid nitrogen storage tank of the present utility model;

FIG. 5 is a cross-sectional view of a biological sample liquid nitrogen storage tank of the present utility model;

FIG. 6 is a left side cross-sectional view of a biological sample liquid nitrogen reservoir containing two trays according to example 1 of the present utility model;

FIG. 7 is a left side cross-sectional view of a biological sample liquid nitrogen reservoir containing four trays according to example 2 of the present utility model;

reference numerals in the drawings are as follows: 1-tank body, 2-sealing cover, 3-storage tray, 4-center rotating shaft, 5-storage tray support, 6-rocker, 7-liquid nitrogen storage bin, 8-sealing cover handle, 9-lighting device, 10-tool placement box, 11-storage tray support hole and 12-heat insulation layer.

Detailed Description

The present utility model will be further described with reference to the accompanying drawings and examples, which are illustrative of the present utility model and the description thereof are not meant to be unduly limiting. It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may explicitly or implicitly include one or more such feature. In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art in a specific case.

Referring to fig. 1-5, the present utility model provides a biological sample liquid nitrogen storage tank: the tank body, the sealing cover, the storage tray and the storage tray rotating mechanism. The figures are as follows: the liquid nitrogen storage tank comprises a tank body 1, a sealing cover 2, a storage tray 3, a central rotating shaft 4, a storage tray bracket 5, a rocker 6, a liquid nitrogen storage bin 7, a sealing cover handle 8, a lighting device 9 and a tool storage box 10. The sealing cover 2 is arranged on the upper portion of the tank body 1, the storage disc 3 is connected with the storage disc rotating mechanism, and the storage disc rotating mechanism is used for adjusting the position of the storage disc 3. The rotating mechanism of the storage disc comprises a central rotating shaft 4, a storage disc bracket 5 and a rocker 6. The central rotating shaft 4 and the storage tray support 5 are positioned in the liquid nitrogen storage bin 7, the liquid nitrogen storage bin 7 further comprises a lighting device 9, and the outside of the tank body is also provided with a sealing cover handle 8 and a tool placement box 10.

In order to better understand the technical scheme of the utility model, the use method of the utility model is described by taking two storage trays and four storage trays as examples.

Example 1 cell access Using a biological sample liquid nitrogen storage tank comprising two trays

Referring to fig. 6, in this embodiment, two trays are used, and two trays are mounted on a tray support. The cells of the desired biological sample are stored in a tray.

1) Firstly, the biological sample liquid nitrogen storage tank 1 is of a sealing structure, and the liquid nitrogen storage bin 7 is filled with liquid nitrogen, and further comprises a storage tray support 5, a central rotating shaft 4, a storage tray 3 and a lighting device 9. The boss on the thing dish is connected with thing dish support hole 11 respectively, and thing dish support 5 is connected with central pivot 4, holds the biological sample cell in putting the thing dish, and the name of marking the cell on every thing dish of putting is convenient to seek. The lighting device is an LED lamp. The rocker 6 is positioned outside the tank body 1 and is connected with the central rotating shaft 4. The tool setting box 10 houses tweezers made of a cold-proof material.

2) Then, under the illumination of LED light, through sealed lid 2, rotate rocker 6, rocker 6 drives center pivot 4 and puts thing dish support 5 and move simultaneously, observe the thing dish distribution condition of putting in the liquid nitrogen storage bin 7. The position of the object placing disc where the needed biological sample cells are located is found according to the corresponding marks on the object placing disc 3, and the needed object placing disc is rotated to the position below the sealing cover 2 and parallel to the sealing cover 2.

3) The protective tool is taken, the sealing cover is covered with the handle 8, the sealing cover 2 of the biological sample liquid nitrogen storage tank is opened, then the tray cover of the storage tray is opened, the biological sample cells are taken out by the disposable clamping tool, and then the tray cover of the storage tray is covered. The sealing cap 2 is then quickly tightened to prevent leakage of liquid nitrogen.

4) After the biological sample cells are used, if the biological sample cells need to be replaced into the storage tank, the operation process is similar to the extraction process, the rocking rod 6 is rotated first, the distribution condition of the storage tray in the liquid nitrogen storage bin 7 is observed, and the position of the storage tray where the required biological sample cells are located is found according to the corresponding marks on the storage tray 3. The required object placing disc is rotated to the lower part of the sealing cover 2, the sealing cover 2 and the cover of the object placing disc are opened, cells are rapidly placed in the object placing disc, the cover on the object placing disc is covered, and then the sealing cover 2 is tightly covered. And taking and storing the cells in the whole biological liquid nitrogen storage tank.

Example 2 cell access Using a biological sample liquid nitrogen storage tank comprising four trays

Referring to fig. 7, in this embodiment, the number of storage trays is four, and the four storage trays are mounted on the storage tray support. The cells of the desired biological sample are stored in a tray.

1) Firstly, the biological sample liquid nitrogen storage tank 1 is of a sealing structure, and the liquid nitrogen storage bin 7 is filled with liquid nitrogen, and further comprises a storage tray support 5, a central rotating shaft 4, a storage tray 3 and a lighting device 9. The boss on the thing dish is connected with thing dish support hole 11 respectively, and thing dish support 5 is connected with central pivot 4, holds the biological sample cell in putting the thing dish, and the name of marking the cell on every thing dish of putting is convenient to seek. The lighting device is an LED lamp for lighting. The rocker 6 is positioned outside the tank body 1 and connected with the central rotating shaft 4. Tweezers of a cold-proof material are placed in the tool placing box 10.

3) The protective tool is taken, the sealing cover is covered with the handle 8, the sealing cover 2 of the biological sample liquid nitrogen storage tank is opened, the tray cover of the storage tray is opened, the biological sample cells are taken out by the disposable clamping tool, and the tray cover of the storage tray is covered. The sealing cap 2 is then quickly tightened to prevent leakage of liquid nitrogen. The process is to take out the biological sample cells from the biological sample liquid nitrogen storage tank.

4) When the biological sample cells are used completely, if the biological sample cells need to be replaced into the storage tank, the operation process is similar to the extraction process, the rocking rod 6 is rotated first, the distribution condition of the storage tray in the liquid nitrogen storage bin 7 is observed, and the position of the storage tray where the required biological sample cells are located is found according to the corresponding marks on the storage tray 3. The required storage tray is rotated to the lower part of the sealing cover 2, the sealing cover 2 and the cover of the storage tray are opened, cells are quickly placed in the storage tray, the cover on the storage tray is covered, and then the sealing cover 2 is covered. And taking and storing the cells in the whole biological liquid nitrogen storage tank.

The foregoing description is only illustrative of the present utility model and is not intended to limit the scope of the utility model, and all equivalent structural changes made by the content of the present utility model or direct or indirect application in other related technical fields are included in the scope of the present utility model.

Claims

1. The biological sample liquid nitrogen storage tank is characterized by comprising a tank body, a sealing cover, a storage disc and a storage disc rotating mechanism;

a liquid nitrogen storage bin is arranged in the tank body;

the sealing cover is arranged at the upper part of the tank body;

the object placing disc rotating mechanism is used for adjusting the position of the object placing disc;

the storage disc rotating mechanism comprises a central rotating shaft penetrating through the tank body in the horizontal direction and a storage disc support, and the central rotating shaft is fixedly connected with the storage disc support; the storage tray is arranged on the storage tray bracket.

2. The biological sample liquid nitrogen storage tank of claim 1, wherein the tray comprises a tray body and a tray cover, and the tray body is detachably and hermetically connected with the tray cover.

3. The biological sample liquid nitrogen storage tank of claim 1, wherein the storage trays are more than two.

4. The biological sample liquid nitrogen storage tank of claim 1, wherein the center of the tray support is provided with a shaft hole, and the center rotating shaft passes through the shaft hole.

5. The biological sample liquid nitrogen storage tank of claim 1, wherein the tray rotation mechanism further comprises a rocker; the rocker is connected with one end of the central rotating shaft, and the rocker is positioned outside the tank body.

6. The biological sample liquid nitrogen storage tank of claim 4, wherein the tray support is provided with a plurality of tray support arms starting from the shaft hole; the object placing tray support arms are radially distributed around the shaft hole as the center.

7. The biological sample liquid nitrogen storage tank of claim 6, wherein the tray support arm is provided with tray support holes; a round boss is arranged on the side surface of the storage disc; the boss is in clearance fit with the bracket hole of the storage disc.

8. The biological sample liquid nitrogen storage tank of claim 1, wherein the inner wall of the tank body is provided with a heat insulation layer.

9. The biological sample liquid nitrogen storage tank of any one of claims 1-8, wherein the sealing cover is made of a transparent material; the tank body is formed by processing high-quality stainless steel.

10. The biological sample liquid nitrogen storage tank of any one of claims 1-8, wherein a tool placement box is further arranged on the tank body; and an illumination device is also arranged in the liquid nitrogen storage bin.