CN216701453U

CN216701453U - Freezing carrying rod for freezing and storing biological material

Info

Publication number: CN216701453U
Application number: CN202123289955.8U
Authority: CN
Inventors: 丁韬力; 万成; 任军
Original assignee: Xukang Medical Science & Technology Suzhou Co ltd
Current assignee: Xukang Medical Science & Technology Suzhou Co ltd
Priority date: 2021-12-24
Filing date: 2021-12-24
Publication date: 2022-06-10
Anticipated expiration: 2031-12-24

Abstract

The utility model provides a freezing carrying rod for freezing and preserving biological materials, wherein a carrying groove for accommodating the biological materials is arranged on the freezing carrying rod, the opening of the carrying groove is circular or elliptical, the bottom of the groove is a biological material storage bin, the biological material storage bin is provided with a circular or elliptical upper opening, the opening area of the biological material storage bin is smaller than that of the groove, an inclined groove side wall is formed between the opening of the groove and the upper opening of the biological material storage bin, the inclined slope of the groove side wall is 50-70 degrees, the length of the groove side wall in the inclined direction is 3-7mm, and the vertical depth from the groove opening to the bottom of the biological material storage bin is 3-7 mm. The freezing loading rod can improve the sealing property and firmness of biological material preservation.

Description

Freezing carrier rod for freezing and preserving biological material

Technical Field

The utility model relates to a freezing carrier rod for freezing and preserving biological materials.

Background

In the field of fertility technology, some biological materials such as somatic cells, sperm, ova, day 1-6 embryos, etc. often require cryopreservation. For example, in tube infant therapy, many of the patients who have taken eggs are not suitable for fresh embryo implantation, and therefore fertilized eggs or blastocysts are frozen for opportunistic implantation when the patient returns to a suitable implantation state. In addition, in order to prevent a failure of one implantation, a temporarily unused ovum taken out at the same time is cryopreserved, and the situation that the ovum needs to be taken out again for a female is prevented, and the like, which all involve the freezing and thawing of the embryo.

Frozen embryos are the only established method for preserving fertility that has been demonstrated. Freezing embryo and freezing liquid are filled into freezing tube, and the embryo can be made to stand and stored in liquid nitrogen at 196 ℃ below zero by two cooling modes of slow (embryo at 2-3 days) and fast (blastocyst at 5-6 days). The artificial uterine tube is implanted into a uterine cavity after being thawed in a natural cycle or an artificial cycle, so that the opportunity of conception of a woman ready for pregnancy is increased. According to statistics, the clinical pregnancy rate of frozen embryo resuscitation is 48.28%, and the clinical pregnancy rate of blastocyst frozen resuscitation is 63.48%.

Fast freezing is more advantageous than slow freezing. Conventional freezing refers to a slow method of slowly cooling the embryo until it eventually freezes, and the major problem with this approach is the formation of ice crystals during freezing that can produce damage to the embryo. The rapid method is also called as a vitrification freezing method, and the vitrification freezing method can avoid the occurrence of ice crystals, improve the success rate of embryo implantation and effectively protect embryos from being damaged in the freezing process. The vitrification freezing method is gradually replacing the conventional method.

The freezing carrying rod is an essential consumable material for the embryo freezing link and is closely related to the freezing-recovery effect of the embryo. Currently most reproductive centers use mainly three types of vitrification freezing bars, open, semi-closed and closed. The open type carrying rod and the semi-closed type carrying rod enable embryos to be in direct contact with liquid nitrogen when the embryos are frozen and stored, cross contamination among the embryos can exist, or potential safety hazards exist on the embryos due to embryo contamination caused by microbial contamination existing in the liquid nitrogen. For the reproductive center, the safety of the embryo is very important, so the use of a closed freezing carrying rod is especially necessary.

CN112674076A discloses a closed type freezing rod, which comprises an inner tube, an outer tube, a tube cap, an air bag, a tapered closing-in and a soft closing ring. When in use, the air bag is matched with the conical closing-in to absorb or lead out sperms and ova; after the absorption, the outer tube is moved, a part of the soft sealing ring arranged at the tapered closing opening is pushed forwards by utilizing the appearance, and the soft sealing ring is inwards extruded and contracted by matching with the appearance, so that the tapered closing opening is sealed; otherwise, the soft sealing ring is opened and reset. However, in the freezing rod, the rod body and the cap material of the rod are not consistent, the surface tension is not uniform, a gap is easily caused by expansion with heat and contraction with cold, and the 'sealing' in the true sense is difficult to realize.

CN209882898U discloses a vitrified freezing straw, which comprises a carrying rod and a sealing sleeve made of the same material, wherein a stepped plug structure is arranged on the carrying rod, so as to realize the sealing between the carrying rod and the sleeve. However, even if the material of the rod body of the carrying rod and the material of the closing cap are the same, the complete close fit of the rod body and the closing cap is difficult to ensure, and the leakage risk still exists.

In addition, the carrying rod in the prior art is not provided with a position for fixing and placing the embryo, but is placed at a certain position of the carrying rod according to the habit of a doctor, and the position of the embryo needs to be searched during observation; there are some loading rods that are simply provided with pits as loading grooves for placing embryos, however, since the loading rods are usually long, the operation needs to be carefully and smoothly performed, otherwise, the embryos are easily ejected or knocked off due to the inclination or vibration of the loading rods, and there is a bad result that the embryos cannot be retrieved when being thawed.

SUMMERY OF THE UTILITY MODEL

It is an object of the present invention to provide a frozen carrier rod for preserving biological material.

The inventor of the present invention has proposed a novel method for cryopreservation of a biological material, wherein the biological material is a somatic cell, sperm, ovum or embryo (D1-D6) or plant cell, etc. from a human or an animal, the method comprising: encapsulating the biological material to be frozen in an oil phase by adopting a water-in-oil technology to form biological material oil drops; and (3) placing the biological material oil drops on a freezing carrying rod, covering inert oil on the biological material oil drops for sealing, and then performing liquid nitrogen freezing preservation.

In order to store the biological materials more conveniently, the utility model provides a freezing carrying rod for freezing and storing the biological materials, the freezing carrying rod is provided with a carrying groove for accommodating the biological materials, the opening of the carrying groove is circular or elliptical, the bottom of the groove is a biological material storage bin, the biological material storage bin is provided with a circular or elliptical upper opening, the opening area of the biological material storage bin is smaller than that of the groove, an inclined groove side wall is formed between the opening of the groove and the upper opening of the biological material storage bin, the inclined slope of the groove side wall is 50-70 degrees, the length of the groove side wall in the inclined direction is 3-7mm, and the vertical depth from the groove opening to the bottom of the biological material storage bin is 3-7 mm.

When the freezing carrier rod for freezing and storing the biological material is applied, the biological material oil drops slide to the biological material storage bin at the bottom along the side wall of the groove, and inert oil is covered on the biological material oil drops for sealing, so that the biological material can be frozen and stored by liquid nitrogen. Wherein, the biomaterial storage bin can also be pre-filled with partial biomaterial protecting liquid or inert oil.

According to the embodiment of the utility model, the freezing carrying rod for freezing and preserving the biological material is characterized in that the opening diameter of the carrying groove is 2-8mm, and the opening diameter of the biological material storage bin is 0.5-3 mm.

In the utility model, when the opening of the carrying groove or the opening of the biological material storage bin is an ellipse, the diameter refers to the major axis and the minor axis of the ellipse, namely, the major axis and the minor axis of the ellipse are both in the diameter size range.

According to the specific embodiment of the utility model, the freezing carrier rod for freezing and preserving the biological material is characterized in that the biological material storage bin is a spherical cavity or a hemispherical cavity with the diameter of 1-3 mm.

According to a specific embodiment of the present invention, the freezing carrier rod for cryopreservation of a biomaterial, wherein the biomaterial storage silo open area is smaller than the maximum cross-sectional area of the biomaterial storage silo. The design is favorable for better covering inert oil on the biomaterial oil drops in the biomaterial storage bin for sealing.

According to a specific embodiment of the present invention, the freezing carrier bar for cryopreservation of biological material, wherein the connection between the inclined groove sidewall and the upper opening of the biological material storage bin is designed as a rounded chamfer with a radius of 2mm or less, such as 0.05-2 mm.

In some embodiments of the utility model, the freezing carrier bar for cryopreservation of biological material of the utility model, wherein the opening of the carrier groove is circular with a diameter of 6mm, the slope of the side wall of the groove is 60 degrees, and the vertical depth from the opening of the groove to the bottom of the biological material storage bin is about 5 mm. The biomaterial storage bin is a hemispherical cavity with the diameter of 3 mm.

According to the embodiment of the utility model, the freezing carrier rod for freezing and preserving the biological material, wherein the inner wall of the carrier groove is made of PPO (polyphenylene oxide), F-4 polytetrafluoroethylene or P1 polyimide, so that the preservation of biological material oil drops is facilitated.

According to the embodiment of the utility model, the freezing support rod for freezing and preserving the biological material is integrally made of PPO (polyphenylene oxide), F-4 polytetrafluoroethylene or P1 polyimide.

According to the specific embodiment of the utility model, the freezing and carrying rod for freezing and preserving the biological material is a cylinder, the length of the freezing and carrying rod is 10-20cm, and the diameter of the freezing and carrying rod is 5-25 mm; the carrying groove is arranged at the position 1-3cm away from the front end of the head of the freezing carrying rod.

According to the embodiment of the utility model, the tail part of the freezing carrying rod for freezing and preserving the biological material is a handheld end, and a biological material sample information bar code area is arranged on the tail part of the freezing carrying rod.

In the present invention, terms used have substantially the same meaning as the related art unless otherwise noted.

For example, in the present invention, the "head" and "tail" of the freezing bar are described as conventional in the art, and the "tail" refers to the hand-held end and the other end is the head (or front end).

For example, in the present invention, the term "bottom" refers to the position of the groove opening upward.

For example, in the present disclosure, the term "spherical" means substantially spherical, and may include, for example, segments having a height above the full sphere height 3/4; "hemispherical" means substantially hemispherical, and may include segments having a height of 3/4-1/4 of a full sphere, for example.

For example, in the present invention, the "inclination of the side wall of the groove" refers to an angle between the inclination direction of the side wall and the horizontal plane when the opening of the groove is upward.

In conclusion, the utility model provides a closed type freezing carrying rod based on a water-in-oil technology, which can greatly improve the sealing property, realize the real 'closing', and avoid the embryo pollution risk caused by contacting liquid nitrogen; the storage position of the biological material is relatively fixed, the biological material is high in storage firmness, and the possibility that the biological material is ejected or knocked down due to the inclination or the vibration of the carrying rod does not exist basically; in addition, the possibility of embryo confusion and error caused by multiple steps in the traditional embryo freezing technology can be avoided.

Drawings

FIG. 1 is a schematic diagram of a cryo-loaded rod for cryopreservation of biological materials according to one embodiment of the utility model.

Fig. 2 is a schematic cross-sectional view of a loading recess of a freezing loading bar for cryopreservation of biological materials according to an embodiment of the present invention.

Detailed Description

For a more clear understanding of the technical features, objects and advantages of the present invention, reference is now made to the following detailed description of the embodiments of the present invention taken in conjunction with the accompanying drawings, which are included to illustrate and not to limit the scope of the present invention.

Example 1

Referring to fig. 1, the present embodiment provides a freezing carrier rod 20 for cryopreservation of biological materials, wherein the freezing carrier rod 20 is integrally made of PPO polyphenylene oxide. The head of the freezing carrier rod 20 is provided with a carrier groove 201 for accommodating the biological material (the opening of the carrier groove 201 is substantially circular). The tail part of the freezing carrying rod 20 is a handheld end and is provided with a biological material sample information bar code area 202.

In this embodiment, the whole freezing carrying rod 20 is a cylinder with a length of 15cm and a diameter of 25 mm; the loading groove 201 is arranged at the position 2cm away from the front end of the head of the freezing loading rod, and the biological material sample information bar code area 202 is arranged at the position 2cm away from the tail end.

Referring to fig. 2 again, in the present embodiment, the opening of the loading recess 201 is circular with a diameter of 6mm, the bottom of the recess is a biomaterial storage silo 2011, the biomaterial storage silo 2011 is a multi-hemispherical cavity with a diameter of 3mm, and has a circular upper opening, an inclined recess sidewall 2012 with a tapered opening is formed between the opening of the recess and the upper opening of the biomaterial storage silo, the inclined slope of the recess sidewall 2012 is 60 degrees, and the vertical depth from the recess opening to the bottom of the biomaterial storage silo is about 5 mm. The connection of the inclined groove side walls to the upper opening of the biomaterial storage silo (as indicated by the arrow in fig. 2) may be designed with a rounded chamfer, which may have a radius of 0.1mm, for example.

When the freezing carrying rod for freezing and storing the biological material is applied, a part (for example, about 1/5 of the volume of the storage bin) of the biological material protection liquid can be filled in the biological material storage bin in advance, then the biological material oil drops slide to the biological material storage bin at the bottom along the side wall of the groove, and then the biological material oil drops are covered with the inert oil for sealing (the inert oil preferably covers the upper opening of the biological material storage bin), so that the biological material can be frozen and stored by liquid nitrogen.

Through tests, the closed type freezing carrying rod of the utility model has no phenomenon that the biological material is popped or knocked down due to the inclination or the vibration of the carrying rod under the conditions of normal storage, transportation and experiments.

Claims

1. A freezing carrying rod for freezing and preserving biological materials is provided with a carrying groove for containing the biological materials, and is characterized in that the opening of the carrying groove is circular or oval, the bottom of the groove is a biological material storage bin, the biological material storage bin is provided with a circular or oval upper opening, the opening area of the biological material storage bin is smaller than that of the groove, an inclined groove side wall is formed between the opening of the groove and the upper opening of the biological material storage bin, the inclined slope of the groove side wall is 50-70 degrees, the length of the groove side wall in the inclined direction is 3-7mm, and the vertical depth from the groove opening to the bottom of the biological material storage bin is 3-7 mm.

2. The freezing carrier bar for cryopreservation of biological material as claimed in claim 1, wherein the opening diameter of the carrier groove is 2-8mm and the opening diameter of the biological material storage bin is 0.5-3 mm.

3. The freezing carrier rod for cryopreservation of biological materials as in claim 1, wherein the biological material storage bin is a spherical or hemispherical cavity with a diameter of 1-3 mm.

4. A freezing bar for the cryopreservation of biological material as in claim 1 or 3 wherein the biological material storage bin open area is less than the maximum cross sectional area of the biological material storage bin.

5. The freezing carrier bar of claim 1 wherein the connection between the inclined sidewall of the groove and the upper opening of the biomaterial storage silo is a rounded chamfer with a radius of 2mm or less.

6. The freezing carrier bar for cryopreservation of biological material as claimed in claim 1, wherein the opening of the carrier groove is circular with a diameter of 6mm, the slope of the side wall of the groove is 60 degrees, and the vertical depth from the opening of the groove to the bottom of the biological material storage bin is 5 mm; the biomaterial storage bin is a hemispherical cavity with the diameter of 3 mm.

7. The freezing carrier bar for cryopreservation of biological materials as in claim 1, wherein the inner wall of the carrier groove is made of PPO, F-4 PTFE or P1 polyimide.

8. The freezing support rod for the cryopreservation of biological materials as claimed in claim 1, wherein the freezing support rod is integrally made of PPO, F-4 polytetrafluoroethylene or P1 polyimide.

9. The freezing bar of claim 1 wherein said freezing bar is generally cylindrical, 10-20cm in length and 5-25mm in diameter; the carrying groove is arranged at the position 1-3cm away from the front end of the head of the freezing carrying rod.

10. The freezing bar of claim 1 or 9, wherein the tail of the freezing bar is a hand-held end, and a barcode region of biological material sample information is arranged on the tail of the freezing bar.