CN108990965A - A kind of cell cryopreservation box - Google Patents
A kind of cell cryopreservation box Download PDFInfo
- Publication number
- CN108990965A CN108990965A CN201810947766.9A CN201810947766A CN108990965A CN 108990965 A CN108990965 A CN 108990965A CN 201810947766 A CN201810947766 A CN 201810947766A CN 108990965 A CN108990965 A CN 108990965A
- Authority
- CN
- China
- Prior art keywords
- box
- box body
- cell cryopreservation
- pore
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 84
- 239000011148 porous material Substances 0.000 claims abstract description 39
- 238000007710 freezing Methods 0.000 claims abstract description 27
- 230000008014 freezing Effects 0.000 claims abstract description 27
- -1 polyethylene Polymers 0.000 claims description 8
- 230000003014 reinforcing effect Effects 0.000 claims description 8
- 239000004698 Polyethylene Substances 0.000 claims description 7
- 229920000573 polyethylene Polymers 0.000 claims description 7
- 238000009826 distribution Methods 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims 2
- 239000007788 liquid Substances 0.000 abstract description 9
- 230000004083 survival effect Effects 0.000 abstract description 9
- 238000010583 slow cooling Methods 0.000 abstract 1
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 16
- 239000006260 foam Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229920001903 high density polyethylene Polymers 0.000 description 2
- 239000004700 high-density polyethylene Substances 0.000 description 2
- 229920001684 low density polyethylene Polymers 0.000 description 2
- 239000004702 low-density polyethylene Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229920001179 medium density polyethylene Polymers 0.000 description 1
- 239000004701 medium-density polyethylene Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Mechanical Engineering (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of cell cryopreservation box, it includes box body and box cover, pore and a fixation hole are frozen for placing the first of cryopreservation tube equipped with several in the tray interior, described first height for freezing pore is less than the height of cell cryopreservation tube, the fixed hole position is in the central location of box body, it is dispersed on the box cover and freezes pore with the first cryopreservation tube hole site corresponding second on box body, box cover central location is distributed fixed convex block corresponding with the fixation hole position, and the box cover fixes convex block and the shapes and sizes of box body fixation hole are mutually matched.Cell cryopreservation box good heat insulating of the invention, enables the temperature of cell cryopreservation tube to be slowly raised and lowered, and ensure that slow cooling of the cell in -80 DEG C of refrigerators, thus ensures higher cell cryopreservation survival rate.Cell cryopreservation box of the present invention, it is environment friendly and pollution-free without the addition of freezing liquid in existing cryopreservation device.
Description
Technical field
The present invention relates to technical field of cell biology, and in particular to a kind of cell cryopreservation tube is dedicated to freeze container.
Background technique
In the passage of cell culture and daily maintenance process, in terms of culture utensil, culture solution and various preparations all
It need to largely expend, and cell once leaves living body and starts originally culture, its various biological natures all will gradually become
Change and constantly has new variation with the increase of passage number and the variation of vitro condition.Therefore cell jelly is carried out in time
It deposits very necessary.Cell cryopreservation is an important link in cell culture.Cryopreservation Technology main points are to freeze slowly, and standard freezes
Program is rate of temperature fall -1~-2 DEG C/min；When temperature reaches -25 DEG C or less, -5 DEG C~-10 DEG C/min can be increased to.Before, often
Conventional method is cryopreservation tube to be placed in 4 DEG C 30 minutes --- > -20 DEG C 60 minutes --- > -80 DEG C overnight --- > liquid nitrogen containers.No
It crosses, since step is more, interval time is again long, it is easy to forget.
Later, people start cryopreservation tube to be put into program temperature reduction box, then program temperature reduction box is put using program temperature reduction box
Enter -80 DEG C of refrigerators.This saves a large amount of time, efficiency is promoted.It is appropriate using being added inside common cell cryopreservation box
Isopropanol, however, isopropanol belongs to toxic solvent, and readily volatilized, long-time service not only be can cause environmental pollution, also right
The safety of experimenter adversely affects.
The programmed cell cooling freezing storing box of the prior art, due to usually requiring that special isopropanol or liquid nitrogen storage is arranged
Chamber, structure is complicated, it is not only possible to pollute environment, and access inconvenience, the cell pipe quantity frozen is very limited, involves great expense, uncomfortable
For a large amount of cell culture.Therefore study practical, the environmentally friendly simple cell cryopreservation box of structure, which have, important applies valence
Value, the present invention come therefrom.
Summary of the invention
The technical problem to be solved by the present invention is to existing cell cryopreservation program temperature reduction box structure is complicated benefits inconvenient for use
And defect not environmentally, a kind of environmentally friendly, easy-to-use cell cryopreservation box are provided.
In order to solve the above technical problems, the present invention provides a kind of cell cryopreservation box comprising box body and box cover, described
Tray interior freezes pore and a fixation hole for placing the first of cryopreservation tube equipped with several, and described first freezes the height of pore
Degree is less than the height of cell cryopreservation tube, the fixed hole position in the central location of box body,
It is dispersed on the box cover and freezes pore with the first cryopreservation tube hole site corresponding second on box body, in box cover
Entreat corresponding with the fixation hole position fixed convex block of position distribution, the shape of the fixed convex block of the box cover and box body fixation hole with
Size is mutually matched.
Preferably, the box body and box cover are polyethylene foam structures.
Preferably, it described first freezes pore and is uniformly distributed on box body, correspondingly, corresponding first freezes pore on box cover
Second freeze pore and be uniformly distributed.
Preferably, it uniformly arranges many walings and cross reinforcing in the outer surface of the box body and box cover.
Preferably, the fixation hole of the box body is cylindrical shape, and the fixation convex block of box cover is the matched circle of shape size therewith
Rod structure.
Preferably, the fixation hole of the box body is rectangular body cavity, and the fixation convex block of box cover is that shape size is matched therewith
Rectangular parallelepiped structure.
Polyethylene of the invention may include low density polyethylene (LDPE), medium density polyethylene, high density polyethylene (HDPE), density model
It encloses from every cubic metre of 28~120 ± 5kg.In actual production, according to different needs, different density range and system may be selected
Standby technique.
Cell cryopreservation box of the present invention, the effect of can obtaining include:
1. cell cryopreservation box good heat insulating of the invention, since the cell cryopreservation tube lower part being placed therein is placed in first
It freezes in pore, what cell cryopreservation tube top was placed on box cover second freezes pore, so that cryopreservation tube sufficiently connects in freezing storing box
Touching sealing, enables the temperature of cell cryopreservation tube to be slowly raised and lowered, ensure that slow drop of the cell in -80 DEG C of refrigerators
Temperature thus ensures higher cell cryopreservation survival rate.
2. cell cryopreservation box of the present invention, due to being polyethylene foam structures, only freezing storing box does not provide good heat insulating ability
Can, and using safe: shatter-resistant, acid-alkali-corrosive-resisting, chemical stability is good, and laboratory environment is suitble to use.
3. cell cryopreservation box of the present invention are environment friendly and pollution-free, do not need that isopropanol or liquid nitrogen storage chamber is in addition arranged, without existing
There is the addition of freezing liquid in cryopreservation device.
4. cell cryopreservation box of the present invention, structure is simple, not only takes conveniently, cost is low, can be according to actual needs
Different box body depth are manufactured, the freezing storing box model of pore height is frozen, is applicable in and promotes the use of, have a vast market foreground and answer
With value.
Detailed description of the invention
The invention will be further described with reference to the accompanying drawings and embodiments:
Fig. 1 is the sectional stereogram of one embodiment of cell cryopreservation box of the present invention.
Fig. 2A is the box body top view of cell cryopreservation box of the present invention, and Fig. 2 B is A-A section partial schematic diagram in Fig. 2A.
Fig. 3 A is the box cover bottom view of cell cryopreservation box of the present invention, and Fig. 3 B is A-A section partial schematic diagram in Fig. 3 A.
Fig. 4 A is the another embodiment box body top view of cell cryopreservation box of the present invention, and Fig. 4 B is to match with Fig. 4 A embodiment box body
Box cover side sectional view.
Fig. 5 is the another embodiment outline drawing of cell cryopreservation box of the present invention.
Fig. 6 is three kinds of cell cryopreservation box freeze-stored cell data comparison figures of the present embodiment 1.
Wherein, 10 be box body, and 11 freeze pore for first, and 12 be fixation hole, and 20 be box cover, and 21 freeze pore for second,
22 be fixed convex block, and H1 is box body depth, and L1 is the first height for freezing pore, and H2 is box cover height, and L2 is the first cryopreservation tube
The height in hole, R are the diameter of fixation hole or fixed convex block, and 310 be waling, and 320 be cross reinforcing.
Specific embodiment
In order to understand technical characteristic and practical effect of the invention in detail, and can come according to the content of specification real
It applies, further with preferred embodiment as indicated in the drawings, detailed description is as follows:
The sectional stereogram of a kind of cell cryopreservation box of the invention as shown in Figure 1 comprising box body 10 and box cover 20, institute
The size shape matching for stating box cover 20 and box body 10 is equipped with several for placing the first of cryopreservation tube inside the box body 10
Pore 11 and a fixation hole 12 are frozen, the fixation hole 12 is located at the central location of box body 10, and described first freezes pore
11 height is less than the height of cell cryopreservation tube, is dispersed with that freeze 11 position of pore with first on box body corresponding on box cover 20
Second freeze pore 21, be distributed fixed convex block 22 corresponding with 12 position of fixation hole in 20 central location of box cover,
The shapes and sizes of the fixation hole 12 of the fixation convex block 22 and box body 10 of box cover 20 are mutually matched.
With reference to Fig. 2A, 3A, Fig. 2A are 10 top view of box body of cell cryopreservation box, and first freezes pore 11 on box body 10
Even distribution, fixation hole 12 are located at the central location of box body 10, and Fig. 3 A is the bottom view of the box cover 20 of cell cryopreservation box, on box cover 20
Second freezes the position that pore 21 corresponding first freezes pore 11, is also uniformly distributed, and 20 central location of box cover is in fixation hole
The corresponding fixed convex block 22 in 12 positions.Fixation hole 12 is cylindrical shape, and fixed convex block 22 is the matched cylinder knot of shape size therewith
Structure.And first freezes pore and second and freezes pore all and be vertical direction setting.
It is A-A section partial schematic diagram in Fig. 2A with reference to Fig. 2 B, 3B, Fig. 2 B, H1 is box body height, and L1 is the first cryopreservation tube
The height in hole 11, H1 > L1, Fig. 3 B are A-A section partial schematic diagram in Fig. 3 A, and H2 is box cover height, and L2 freezes pore for first
21 height, H2 > L2, in the present embodiment, fixation hole 12 is cylindrical shape, and fixed convex block 22 is the matched circle of shape size therewith
Rod structure, and the diameter of the two is equal for R.
In the preferred embodiment of the present invention, first to freeze pore internal diameter consistent with cell cryopreservation pipe outside diameter, and the first cryopreservation tube
Hole height L1 is less than the height of cell cryopreservation tube, in this way, the cell cryopreservation tube lower part being placed therein, which is placed in first, freezes pore
In 11, cell cryopreservation tube top is placed in 21 hole of the second cryopreservation tube on box cover, so that cryopreservation tube comes into full contact with sealing in box body,
The temperature of cell cryopreservation tube is set slowly to be raised and lowered, it is ensured that higher cell cryopreservation survival rate.
In the present invention, for cell cryopreservation box because of no setting is required freezing liquid storage chamber, freezing storing box is non-hollow structure, and
It is preferred that box body and box cover are polyethylene foam structures.It is not only that freezing storing box provides good thermal insulation property, and shatter-resistant, uses peace
It is complete: to have the advantages that chemical stability is good, acid-fast alkali-proof, be suitable for laboratory and use.
In one embodiment of the invention, the fixation hole of the box body is a rectangular body cavity, and the fixation convex block of box cover is shape therewith
The matched rectangular parallelepiped structure of shape size.In one embodiment of the invention, the fixation hole of the box body is inverted round stage structure, and box cover is consolidated
Convex block is determined for the matched frustum of a cone of shape size therewith.Certainly can also be it may occur to persons skilled in the art that other mutually
Fit structure or curved surface.
Fig. 4 A is the another embodiment box body top view of cell cryopreservation box, and Fig. 4 B is and the matched box cover of Fig. 4 A embodiment box body
Side sectional view and periphery above-mentioned are that columned cell cryopreservation box are different, and in the embodiment, the periphery of cell cryopreservation box is
Rectangle, and fixation hole 12 is a rectangular body cavity, the fixation convex block 22 of box 20 is the matched rectangular parallelepiped structure of shape size therewith.
Fig. 5 is the another embodiment outline drawing of cell cryopreservation box of the present invention, in the uniformly arrangement in appearance of the cell cryopreservation box
Many walings 310 and cross reinforcing 320, in this way, each vertical and horizontal reinforcing rib 310,320 cooperates jointly,
So as to reinforce cell cryopreservation box in the intensity of vertical and horizontal, further, each waling 310 freezes along cell
Deposit the short transverse equidistant placement of box, each 320 horizontal direction equidistant placement of cross reinforcing.
Further, each waling and cross reinforcing and cell cryopreservation box bond, naturally it is also possible to
Cell cryopreservation box are made of one structure.And resin material or polyethylene can be used in reinforcing rib.
According to practical application, cell cryopreservation box are different depth: for freezing for 58mm according to the height of cryopreservation tube
Pipe, freezing storing box box body height H1 are 60mm, and first freezes pore L1 height as 38mm, and box cover height H2 is 40mm, and first freezes
Pore L2 height is 20mm；
For the cryopreservation tube of 80mm height, freezing storing box box body height H1 is 82mm, and first to freeze pore L1 height be 60mm,
Box cover height H2 is 40mm, and first freezes pore L2 height as 20mm；
For the cryopreservation tube of 102mm height, freezing storing box box body height H1 is 82mm, and first, which freezes pore L1 height, is
82mm, box cover height H2 are 40mm, and first freezes pore L2 height as 20mm.
Therefore, cryopreservation tube of the invention, according to different height cryopreservation tube, it is only necessary to adjust the knot of one of box body or box cover
Structure, flexibly collocation can meet needs, and use is safe, quite facilitates practical.
Embodiment 1
Using three kinds of different cell cryopreservation box, (isopropanol freezing storing box, NEB freezing storing box, polyethylene of the invention freeze
Deposit box) freeze HEK293 cell (my military biotech firm), the specific steps are as follows:
1. cell passes on
1.1 take out frozen stock solution, PBS, Tripsn, culture solution from refrigerator.It is placed on pre-temperature in water-bath.
Fill the culture dish of HEK293 cell in 1.2 taking-up incubators, microscopically observation plating cells rate up to 80%~
90%, the waste liquid in culture dish is absorbed using waste liquid rifle, takes 3mlPBS solution gently to shake along culture dish using electric rifle and washes cell, then
Draw PBS waste liquid.
1.3 take 1.5ml Tripsn that culture dish is added along edge, and light shake makes cellular infiltration in Tripsn.Under microscope
Whether observation cell all infiltrates, and puts 1~2min of incubator into.
1.4 light shake observe whether cell all loosens, and the culture solution that 3ml is added, which gently shakes gently piping and druming, makes cell uniformly dispersing
In the medium, digestion reaction is terminated.
1.5 take the centrifuge tube of a 15ml, label.3ml culture is drawn out of culture medium loaded in centrifuge tube, is centrifuged
4000rpm, 10min.
1.6 are added 5ml cell culture fluid, are resuspended, cell count, every 500 × 104 cell cryopreservations.It is respectively charged into difference
Cell cryopreservation box in.Place -80 DEG C of refrigerators.
1.7 take out freeze-stored cell every other day, recovery, and cell count calculates cell survival rate.
Freeze-stored cell as shown in table 1, every kind freezes 6 groups, each group freeze-stored cell 500 × 104, finally carry out statistical
Analysis.
Table 1
| Isopropanol freezing storing box | NEB freezing storing box | Foam freezing storing box | |
| Number | 1~6 group | 7~12 groups | 13~18 groups |
Cell cryopreservation survival rate: such as table 2
| Freeze-stored cell number | Recovery survival number | Recovery survival rate | Cell number after culture 6h | After 6h/freeze number | After 6h/recovery survival number | |
| Isopropanol freezing storing box | 500×104 | 497.5×104 | 99.50% | 465×104 | 93% | 93.50% |
| NEB freezing storing box | 500×104 | 372×104 | 74.40% | 346×104 | 69.2% | 93.01% |
| Foam freezing storing box | 500×104 | 467×104 | 93.40% | 447×104 | 89.40% | 95.70% |
Obtained by experimental data: foam freezing storing box and isopropanol freezing storing box cell survival rate are very nearly the same, in contrast, this
The foam freezing storing box of invention is more advantageous.
The above is only the preferred embodiment of the present invention, is not intended to limit the present invention in any form, Ren Hesuo
Belong in technical field and have usually intellectual, if without departing from the scope of the technical features of the present invention, utilizing institute of the present invention
The equivalent embodiment that locally changes or modify made by technology contents is disclosed, in the range of still falling within the technology of the present invention feature.
Claims (6)
1. a kind of cell cryopreservation box comprising box body and box cover, which is characterized in that
The tray interior be equipped with it is several freeze pore and a fixation hole for placing the first of cryopreservation tube, described first
The height for freezing pore is less than the height of cell cryopreservation tube, the fixed hole position in the central location of box body,
It is dispersed on the box cover and freezes pore, box cover center position with the first cryopreservation tube hole site corresponding second on box body
Set distribution fixed convex block corresponding with the fixation hole position, the shapes and sizes of the box cover fixed convex block and box body fixation hole
It is mutually matched.
2. cell cryopreservation box according to claim 1, which is characterized in that the box body and box cover is polyethylene knot
Structure.
3. cell cryopreservation box according to claim 1, which is characterized in that described first freezes pore uniformly divides on box body
Cloth, correspondingly, corresponding first freezes the second of pore and freezes pore and be uniformly distributed on box cover.
4. cell cryopreservation box according to claim 1, which is characterized in that uniformly arranged in the outer surface of the box body and box cover
Cloth many walings and cross reinforcing.
5. cell cryopreservation box according to claim 1, which is characterized in that the fixation hole of the box body is cylindrical shape, box cover
Fixation convex block be the matched cylindrical structure of shape size therewith.
6. cell cryopreservation box according to claim 1, which is characterized in that the fixation hole of the box body is rectangular body cavity, box
The fixation convex block of lid is the matched rectangular parallelepiped structure of shape size therewith.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810947766.9A CN108990965A (en) | 2018-08-20 | 2018-08-20 | A kind of cell cryopreservation box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810947766.9A CN108990965A (en) | 2018-08-20 | 2018-08-20 | A kind of cell cryopreservation box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108990965A true CN108990965A (en) | 2018-12-14 |
Family
ID=64593292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810947766.9A Pending CN108990965A (en) | 2018-08-20 | 2018-08-20 | A kind of cell cryopreservation box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108990965A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111268241A (en) * | 2020-03-11 | 2020-06-12 | 中国科学院大学宁波华美医院 | Cell freezing box |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009157585A1 (en) * | 2008-06-24 | 2009-12-30 | 独立行政法人海洋研究開発機構 | Support for freeze preservation of animal cells and biodevice for freeze preservation and method for freeze preservation using the same |
| WO2012032853A1 (en) * | 2010-09-08 | 2012-03-15 | 浜松ホトニクス株式会社 | Method for preparation of algal cells, and kit for evaluation of toxicity of chemical substance |
| CN203040527U (en) * | 2013-02-25 | 2013-07-10 | 中国水产科学研究院黑龙江水产研究所 | Cell freezing and storing box |
| CN204426477U (en) * | 2015-01-23 | 2015-07-01 | 马建波 | A kind of Simple cell program freezing storing box |
| CN204998927U (en) * | 2015-08-20 | 2016-01-27 | 中山市泰威技术开发有限公司 | Quartzy quartz monitor crystal packing carton |
| CN206808534U (en) * | 2017-05-27 | 2017-12-29 | 云南新生命干细胞有限公司 | A kind of stem cell cryopreserving box |
-
2018
- 2018-08-20 CN CN201810947766.9A patent/CN108990965A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009157585A1 (en) * | 2008-06-24 | 2009-12-30 | 独立行政法人海洋研究開発機構 | Support for freeze preservation of animal cells and biodevice for freeze preservation and method for freeze preservation using the same |
| WO2012032853A1 (en) * | 2010-09-08 | 2012-03-15 | 浜松ホトニクス株式会社 | Method for preparation of algal cells, and kit for evaluation of toxicity of chemical substance |
| CN203040527U (en) * | 2013-02-25 | 2013-07-10 | 中国水产科学研究院黑龙江水产研究所 | Cell freezing and storing box |
| CN204426477U (en) * | 2015-01-23 | 2015-07-01 | 马建波 | A kind of Simple cell program freezing storing box |
| CN204998927U (en) * | 2015-08-20 | 2016-01-27 | 中山市泰威技术开发有限公司 | Quartzy quartz monitor crystal packing carton |
| CN206808534U (en) * | 2017-05-27 | 2017-12-29 | 云南新生命干细胞有限公司 | A kind of stem cell cryopreserving box |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111268241A (en) * | 2020-03-11 | 2020-06-12 | 中国科学院大学宁波华美医院 | Cell freezing box |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102858947B (en) | Cell culture system | |
| CN103270149B (en) | Cell culture insert | |
| EP2345714A1 (en) | Cell storage method and cell transport method | |
| KR20070102589A (en) | Support device for cells culture | |
| US9850458B2 (en) | Bioreactor with lid for easy access to incubation cavity | |
| CN108990965A (en) | A kind of cell cryopreservation box | |
| Sousa et al. | One‐step rapid fabrication of cell‐only living fibers | |
| CN209047297U (en) | Cell cryopreservation box | |
| CN100560710C (en) | High-density array type cell slide culture apparatus, storage facility and experimental installation | |
| CN104450505B (en) | A kind of tissue block method culture dish | |
| CN204362811U (en) | A kind of freezing storing box depositing cell cryopreservation bag | |
| CN107629941A (en) | A kind of cell cryopreservation device and its application based on micro-fluidic chip | |
| CN210856118U (en) | Molecular biology culture dish | |
| CN201501859U (en) | Fungal microculture device capable of being directly observed under microscope | |
| CN207943383U (en) | A kind of multifunctional low-temperature storage biological sample device | |
| US20190024032A1 (en) | Tissue cell culture device | |
| CN204799313U (en) | A centrifuging tube keeps box for liquid nitrogen quick -freeze | |
| CN204198747U (en) | A kind of cell culture bags of convenient operation | |
| CN203551351U (en) | Device for preparing ice sample for ice-smelting ability test of snow-melting agent | |
| CN204882182U (en) | Retrieve pore solution's in transparent soil device fast | |
| CN205884527U (en) | Freeze and deposit pipe | |
| CN205223256U (en) | Warm box of constant temperature | |
| CN105802852A (en) | Biological scaffold perfusion culture room and biological scaffold perfusion culture device | |
| CN218539666U (en) | Detachable trace cell culture orifice plate device and culture dish | |
| CN205258487U (en) | Novel culture dish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |